Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568900 | KYP58465.1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 5 | 140 | 1.76e-92 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568900 | sp|P68428|H32_WHEAT | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 5.15e-95 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568900 | A0A1D1ZGP1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 1.97e-92 | 272 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0113410 | Msa0568900 | 0.805612 | 1.227315e-49 | -8.615850e-47 |
| Msa0412130 | Msa0568900 | 0.809064 | 2.276036e-50 | -8.615850e-47 |
| Msa0553210 | Msa0568900 | 0.816202 | 6.246273e-52 | -8.615850e-47 |
| Msa0561560 | Msa0568900 | 0.802336 | 5.888525e-49 | -8.615850e-47 |
| Msa0563980 | Msa0568900 | 0.801138 | 1.037038e-48 | -8.615850e-47 |
| Msa0568710 | Msa0568900 | 0.862711 | 4.177330e-64 | -8.615850e-47 |
| Msa0568830 | Msa0568900 | 0.956544 | 2.298605e-114 | -8.615850e-47 |
| Msa0568850 | Msa0568900 | 0.904219 | 1.542294e-79 | -8.615850e-47 |
| Msa0568900 | Msa0575710 | 0.813895 | 2.030286e-51 | -8.615850e-47 |
| Msa0568900 | Msa0645340 | 0.844925 | 5.590215e-59 | -8.615850e-47 |
| Msa0568900 | Msa0688620 | 0.809942 | 1.474599e-50 | -8.615850e-47 |
| Msa0568900 | Msa0695560 | 0.801882 | 7.299891e-49 | -8.615850e-47 |
| Msa0568900 | Msa0749190 | 0.806438 | 8.224037e-50 | -8.615850e-47 |
| Msa0568900 | Msa0750520 | 0.806223 | 9.128781e-50 | -8.615850e-47 |
| Msa0568900 | Msa0965470 | 0.800144 | 1.653808e-48 | -8.615850e-47 |
| Msa0568900 | Msa1071410 | 0.808584 | 2.882184e-50 | -8.615850e-47 |
| Msa0568900 | Msa1082380 | 0.801154 | 1.029493e-48 | -8.615850e-47 |
| Msa0568900 | Msa1162880 | 0.803000 | 4.294409e-49 | -8.615850e-47 |
| Msa0568900 | Msa1296330 | 0.803623 | 3.191714e-49 | -8.615850e-47 |
| Msa0568900 | Msa1381800 | 0.808493 | 3.014543e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568900 | MtrunA17_Chr5g0410931 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr5g0410951 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr8g0383851 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr8g0383781 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr8g0390361 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr2g0295191 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr7g0237661 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr4g0033041 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr7g0218971 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr4g0046301 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr8g0364801 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568900 | MtrunA17_Chr4g0054161 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0568900 | MtrunA17_Chr8g0363741 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0568900 | MtrunA17_Chr1g0156021 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0568900 | MtrunA17_Chr4g0054151 | 97.059 | 136 | 4 | 0 | 1 | 136 | 4 | 139 | 2.61e-93 | 265 |
| Msa0568900 | MtrunA17_Chr8g0388121 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.09e-92 | 264 |
| Msa0568900 | MtrunA17_Chr2g0319141 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0568900 | MtrunA17_Chr2g0319151 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0568900 | MtrunA17_Chr2g0319131 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.38e-82 | 238 |
| Msa0568900 | MtrunA17_Chr2g0319121 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 5.82e-75 | 219 |
| Msa0568900 | MtrunA17_Chr7g0218961 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 8.01e-66 | 195 |
| Msa0568900 | MtrunA17_Chr7g0222991 | 67.164 | 134 | 43 | 1 | 1 | 134 | 10 | 142 | 5.47e-56 | 171 |
| Msa0568900 | MtrunA17_Chr5g0407311 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.29e-45 | 144 |
| Msa0568900 | MtrunA17_Chr7g0223001 | 58.647 | 133 | 41 | 3 | 3 | 134 | 27 | 146 | 5.46e-43 | 139 |
| Msa0568900 | MtrunA17_Chr7g0240791 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 9.61e-41 | 133 |
| Msa0568900 | MtrunA17_Chr8g0347231 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 3.80e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568900 | AT1G09200.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568900 | AT5G65360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568900 | AT5G10390.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568900 | AT5G10400.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568900 | AT3G27360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568900 | AT4G40030.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT4G40030.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT4G40030.4 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT5G10980.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT4G40040.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT4G40040.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT4G40040.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568900 | AT4G40030.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 7.05e-93 | 266 |
| Msa0568900 | AT5G65350.1 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 5.30e-91 | 260 |
| Msa0568900 | AT1G75600.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.25e-89 | 256 |
| Msa0568900 | AT1G13370.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 5.55e-89 | 254 |
| Msa0568900 | AT1G19890.1 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.07e-83 | 241 |
| Msa0568900 | AT5G12910.1 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 9.90e-67 | 198 |
| Msa0568900 | AT1G01370.2 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0568900 | AT1G01370.1 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0568900 | AT1G01370.4 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0568900 | AT1G01370.3 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
Find 38 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGGTTGCCAGCTGCTTC+CGG | 0.174491 | 4_1:+70515446 | None:intergenic |
| CGGTCGCCGGAGCTGATTTC+CGG | 0.242180 | 4_1:+70515419 | None:intergenic |
| TTTGGTTGCCAGCTGCTTCC+GGG | 0.259335 | 4_1:+70515447 | None:intergenic |
| AGCTGATTTCCGGGCGGCTT+TGG | 0.313671 | 4_1:+70515429 | None:intergenic |
| TCTCTCAAAGCAACTGTTCC+AGG | 0.333816 | 4_1:+70515367 | None:intergenic |
| CAGTGCTGTATCTGCTCTTC+AGG | 0.340320 | 4_1:-70515236 | Msa0568900:CDS |
| GGTCGCCGGAGCTGATTTCC+GGG | 0.345559 | 4_1:+70515420 | None:intergenic |
| GGAAGCTGCTGAGGCTTACT+TGG | 0.361475 | 4_1:-70515215 | Msa0568900:CDS |
| CAAGGCTCCCCGGAAGCAGC+TGG | 0.396825 | 4_1:-70515455 | Msa0568900:CDS |
| TTCTTCACTCCTCCGGTCGC+CGG | 0.442119 | 4_1:+70515406 | None:intergenic |
| GCTGCTGAGGCTTACTTGGT+TGG | 0.450601 | 4_1:-70515211 | Msa0568900:CDS |
| GACGTTGGAATGGAAGTTTA+CGG | 0.458530 | 4_1:+70515308 | None:intergenic |
| CTTGCAAGCTGAATGTCCTT+AGG | 0.462646 | 4_1:+70515133 | None:intergenic |
| CGGAAATCAGCTCCGGCGAC+CGG | 0.464380 | 4_1:-70515418 | Msa0568900:CDS |
| CCACCGGCGGCAAGGCTCCC+CGG | 0.480154 | 4_1:-70515465 | Msa0568900:CDS |
| TCGCAAGTCCACCGGCGGCA+AGG | 0.488583 | 4_1:-70515473 | Msa0568900:CDS |
| AGCTGGCAACCAAAGCCGCC+CGG | 0.490048 | 4_1:-70515438 | Msa0568900:CDS |
| AGCCGCCCGGAAATCAGCTC+CGG | 0.497667 | 4_1:-70515425 | Msa0568900:CDS |
| GTGTGGCTTCTTCACTCCTC+CGG | 0.499925 | 4_1:+70515399 | None:intergenic |
| CAGTGCTCTTCTGATACTTA+CGG | 0.501233 | 4_1:+70515341 | None:intergenic |
| CTTCCGGGGAGCCTTGCCGC+CGG | 0.507682 | 4_1:+70515462 | None:intergenic |
| TTGGTTGCCAGCTGCTTCCG+GGG | 0.508384 | 4_1:+70515448 | None:intergenic |
| TGCTCTTCAGGAAGCTGCTG+AGG | 0.517538 | 4_1:-70515224 | Msa0568900:CDS |
| CCGGGGAGCCTTGCCGCCGG+TGG | 0.548377 | 4_1:+70515465 | None:intergenic |
| GGACTTGCGAGCAGTCTGCT+TGG | 0.551577 | 4_1:+70515486 | None:intergenic |
| CAGACTGCTCGCAAGTCCAC+CGG | 0.562052 | 4_1:-70515481 | Msa0568900:CDS |
| GAGAGTTACAATCATGCCTA+AGG | 0.570792 | 4_1:-70515149 | Msa0568900:CDS |
| AAGAAGAATCAGAGGAGAGA+GGG | 0.573886 | 4_1:-70515113 | Msa0568900:CDS |
| TCACGAACAAGACGTTGGAA+TGG | 0.591294 | 4_1:+70515298 | None:intergenic |
| AAATCAGCTCCGGCGACCGG+AGG | 0.599225 | 4_1:-70515415 | Msa0568900:CDS |
| CAAGAAGAATCAGAGGAGAG+AGG | 0.603703 | 4_1:-70515114 | Msa0568900:CDS |
| CAATTTCACGAACAAGACGT+TGG | 0.614742 | 4_1:+70515293 | None:intergenic |
| CAGCTTGCAAGAAGAATCAG+AGG | 0.621034 | 4_1:-70515121 | Msa0568900:CDS |
| AAGATTTCAAGACAGATCTG+AGG | 0.632208 | 4_1:-70515267 | Msa0568900:CDS |
| ACTGCTCGCAAGTCCACCGG+CGG | 0.647164 | 4_1:-70515478 | Msa0568900:CDS |
| AAGCCACACAGATTCAGACC+TGG | 0.672238 | 4_1:-70515385 | Msa0568900:CDS |
| GTTCCAGGTCTGAATCTGTG+TGG | 0.688220 | 4_1:+70515382 | None:intergenic |
| CGCCGGAGCTGATTTCCGGG+CGG | 0.701307 | 4_1:+70515423 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGATTTCAAGACAGATCTG+AGG | - | chr4_1:70515337-70515356 | Msa0568900:CDS | 35.0% | |
| CAGTGCTCTTCTGATACTTA+CGG | + | chr4_1:70515266-70515285 | None:intergenic | 40.0% | |
| GACGTTGGAATGGAAGTTTA+CGG | + | chr4_1:70515299-70515318 | None:intergenic | 40.0% | |
| CAATTTCACGAACAAGACGT+TGG | + | chr4_1:70515314-70515333 | None:intergenic | 40.0% | |
| GAGAGTTACAATCATGCCTA+AGG | - | chr4_1:70515455-70515474 | Msa0568900:CDS | 40.0% | |
| AAGAAGAATCAGAGGAGAGA+GGG | - | chr4_1:70515491-70515510 | Msa0568900:CDS | 40.0% | |
| TCTCTCAAAGCAACTGTTCC+AGG | + | chr4_1:70515240-70515259 | None:intergenic | 45.0% | |
| TCACGAACAAGACGTTGGAA+TGG | + | chr4_1:70515309-70515328 | None:intergenic | 45.0% | |
| CTTGCAAGCTGAATGTCCTT+AGG | + | chr4_1:70515474-70515493 | None:intergenic | 45.0% | |
| CAGCTTGCAAGAAGAATCAG+AGG | - | chr4_1:70515483-70515502 | Msa0568900:CDS | 45.0% | |
| CAAGAAGAATCAGAGGAGAG+AGG | - | chr4_1:70515490-70515509 | Msa0568900:CDS | 45.0% | |
| AAGCCACACAGATTCAGACC+TGG | - | chr4_1:70515219-70515238 | Msa0568900:CDS | 50.0% | |
| GTTCCAGGTCTGAATCTGTG+TGG | + | chr4_1:70515225-70515244 | None:intergenic | 50.0% | |
| CAGTGCTGTATCTGCTCTTC+AGG | - | chr4_1:70515368-70515387 | Msa0568900:CDS | 50.0% | |
| TTTGGTTGCCAGCTGCTTCC+GGG | + | chr4_1:70515160-70515179 | None:intergenic | 55.0% | |
| CTTTGGTTGCCAGCTGCTTC+CGG | + | chr4_1:70515161-70515180 | None:intergenic | 55.0% | |
| GTGTGGCTTCTTCACTCCTC+CGG | + | chr4_1:70515208-70515227 | None:intergenic | 55.0% | |
| TGCTCTTCAGGAAGCTGCTG+AGG | - | chr4_1:70515380-70515399 | Msa0568900:CDS | 55.0% | |
| GGAAGCTGCTGAGGCTTACT+TGG | - | chr4_1:70515389-70515408 | Msa0568900:CDS | 55.0% | |
| GCTGCTGAGGCTTACTTGGT+TGG | - | chr4_1:70515393-70515412 | Msa0568900:CDS | 55.0% | |
| GGACTTGCGAGCAGTCTGCT+TGG | + | chr4_1:70515121-70515140 | None:intergenic | 60.0% | |
| CAGACTGCTCGCAAGTCCAC+CGG | - | chr4_1:70515123-70515142 | Msa0568900:CDS | 60.0% | |
| TTGGTTGCCAGCTGCTTCCG+GGG | + | chr4_1:70515159-70515178 | None:intergenic | 60.0% | |
| ! | AGCTGATTTCCGGGCGGCTT+TGG | + | chr4_1:70515178-70515197 | None:intergenic | 60.0% |
| TTCTTCACTCCTCCGGTCGC+CGG | + | chr4_1:70515201-70515220 | None:intergenic | 60.0% | |
| ACTGCTCGCAAGTCCACCGG+CGG | - | chr4_1:70515126-70515145 | Msa0568900:CDS | 65.0% | |
| AGCTGGCAACCAAAGCCGCC+CGG | - | chr4_1:70515166-70515185 | Msa0568900:CDS | 65.0% | |
| AGCCGCCCGGAAATCAGCTC+CGG | - | chr4_1:70515179-70515198 | Msa0568900:CDS | 65.0% | |
| ! | GGTCGCCGGAGCTGATTTCC+GGG | + | chr4_1:70515187-70515206 | None:intergenic | 65.0% |
| ! | CGGTCGCCGGAGCTGATTTC+CGG | + | chr4_1:70515188-70515207 | None:intergenic | 65.0% |
| CGGAAATCAGCTCCGGCGAC+CGG | - | chr4_1:70515186-70515205 | Msa0568900:CDS | 65.0% | |
| AAATCAGCTCCGGCGACCGG+AGG | - | chr4_1:70515189-70515208 | Msa0568900:CDS | 65.0% | |
| TCGCAAGTCCACCGGCGGCA+AGG | - | chr4_1:70515131-70515150 | Msa0568900:CDS | 70.0% | |
| CAAGGCTCCCCGGAAGCAGC+TGG | - | chr4_1:70515149-70515168 | Msa0568900:CDS | 70.0% | |
| ! | CGCCGGAGCTGATTTCCGGG+CGG | + | chr4_1:70515184-70515203 | None:intergenic | 70.0% |
| CTTCCGGGGAGCCTTGCCGC+CGG | + | chr4_1:70515145-70515164 | None:intergenic | 75.0% | |
| ! | CCACCGGCGGCAAGGCTCCC+CGG | - | chr4_1:70515139-70515158 | Msa0568900:CDS | 80.0% |
| ! | CCGGGGAGCCTTGCCGCCGG+TGG | + | chr4_1:70515142-70515161 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 70515108 | 70515518 | 70515108 | ID=Msa0568900;Name=Msa0568900 |
| chr4_1 | mRNA | 70515108 | 70515518 | 70515108 | ID=Msa0568900-mRNA-1;Parent=Msa0568900;Name=Msa0568900-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr4_1 | exon | 70515108 | 70515518 | 70515108 | ID=Msa0568900-mRNA-1:exon:23810;Parent=Msa0568900-mRNA-1 |
| chr4_1 | CDS | 70515108 | 70515518 | 70515108 | ID=Msa0568900-mRNA-1:cds;Parent=Msa0568900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
