AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0576380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576380 MtrunA17_Chr8g0390301 90.667 225 14 4 1 223 1 220 1.37e-134 382
Msa0576380 MtrunA17_Chr6g0486111 53.333 240 66 12 1 223 1 211 1.28e-61 196
Msa0576380 MtrunA17_Chr2g0331431 40.851 235 91 12 15 223 1 213 3.26e-31 117
Msa0576380 MtrunA17_Chr2g0280631 62.222 45 17 0 179 223 284 328 7.81e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576380 AT4G29100.2 36.513 304 95 16 1 223 1 287 1.14e-27 108
Msa0576380 AT4G29100.1 36.513 304 95 16 1 223 1 287 1.88e-27 108
Msa0576380 AT2G20100.4 42.623 122 43 5 106 223 145 243 1.84e-12 65.9
Msa0576380 AT2G20100.2 42.623 122 43 5 106 223 145 243 1.84e-12 65.9
Msa0576380 AT2G20100.1 42.623 122 43 5 106 223 145 243 2.42e-12 65.9
Msa0576380 AT2G20100.3 42.623 122 43 5 106 223 145 243 3.11e-12 65.9

Find 78 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCTTCCTCTTCCTTCT+TGG 0.083493 4_1:-80455579 Msa0576380:CDS
TAATATGAATTTAATAAATT+AGG 0.246937 4_1:+80454141 None:intergenic
AGAGAAGATGCATGAGGATA+TGG 0.253894 4_1:+80455595 None:intergenic
ATTTGAGACCAAGTAGAATT+TGG 0.254844 4_1:+80455036 None:intergenic
ATGGTGTTCCACCAGTTGTT+AGG 0.275388 4_1:+80455694 None:intergenic
CATCAACTCGTTTCCCCATT+TGG 0.284889 4_1:-80454183 Msa0576380:CDS
TCTTTCTTGATGCTTCAAAT+AGG 0.285345 4_1:+80455861 None:intergenic
CTTTCTTGATGCTTCAAATA+GGG 0.296957 4_1:+80455862 None:intergenic
GAAACTTGTTGCACAGGATT+TGG 0.306086 4_1:+80454990 None:intergenic
GTAGTAGTGGAGAAGAAATT+AGG 0.320001 4_1:+80455640 None:intergenic
GTGACACACTTAATATTAAT+CGG 0.330980 4_1:+80453985 None:intergenic
GGAGTAAGAAAATTAGAATT+AGG 0.340867 4_1:+80455616 None:intergenic
CAGGTTAGGAAGGAGAAGTT+AGG 0.364935 4_1:-80454225 Msa0576380:CDS
CCAGCCATCATTTGTTGCAC+TGG 0.375597 4_1:+80455721 None:intergenic
TGGTTGGTTAAGAAGCTATA+GGG 0.384219 4_1:+80455920 None:intergenic
CTCTTGTCTTTGTAGAATCT+AGG 0.384228 4_1:-80455795 Msa0576380:five_prime_UTR
TCAGTGAACAACTATGTCTA+TGG 0.389055 4_1:-80455081 Msa0576380:CDS
TATATATTCACCTTTCCAAA+TGG 0.397753 4_1:+80454168 None:intergenic
TATGGAAATGAAGAATTTCA+TGG 0.412434 4_1:-80455063 Msa0576380:CDS
GTGGTTGGTTAAGAAGCTAT+AGG 0.436252 4_1:+80455919 None:intergenic
ATATATTCACCTTTCCAAAT+GGG 0.441398 4_1:+80454169 None:intergenic
ATCAGATCTATCTTCTGATC+AGG 0.442936 4_1:-80454911 Msa0576380:intron
TGGTGTTCCACCAGTTGTTA+GGG 0.450899 4_1:+80455695 None:intergenic
TACAACTCAGTCTACATTCA+AGG 0.451223 4_1:-80454479 Msa0576380:intron
TGACACACTTAATATTAATC+GGG 0.458902 4_1:+80453986 None:intergenic
GGATTTCTCTAACACTAAAA+CGG 0.468693 4_1:-80454950 Msa0576380:CDS
ATGGAACACCAAATTCTACT+TGG 0.470969 4_1:-80455044 Msa0576380:CDS
ATTAGAATTAGGAGTAGTAG+TGG 0.475544 4_1:+80455627 None:intergenic
AGGAGGCTGACTTAGCATAC+TGG 0.481806 4_1:+80455140 None:intergenic
TCTTGTCTTTGTAGAATCTA+GGG 0.484970 4_1:-80455794 Msa0576380:five_prime_UTR
CTCACCAGTGCAACAAATGA+TGG 0.487107 4_1:-80455725 Msa0576380:CDS
TCTATCAAAGTTTCTACAAA+GGG 0.490114 4_1:-80455889 Msa0576380:five_prime_UTR
GTGGGGCATTGAAGAAAGCT+AGG 0.491010 4_1:-80454513 Msa0576380:CDS
TTGATAGATGCTTCTGTGGT+TGG 0.497737 4_1:+80455904 None:intergenic
GAGAGGAAACAAAAGACAAA+AGG 0.499920 4_1:-80456021 None:intergenic
TTAGGTTGATGATGATGATC+AGG 0.504746 4_1:+80455658 None:intergenic
GAAGGAACACTATTGTTGCT+TGG 0.508525 4_1:+80455944 None:intergenic
CCAGTGCAACAAATGATGGC+TGG 0.516782 4_1:-80455721 Msa0576380:CDS
GTAATAGCTCTTCAGCTGGT+GGG 0.518744 4_1:-80454531 Msa0576380:CDS
TGGCTGGAAACCCTAACAAC+TGG 0.519094 4_1:-80455705 Msa0576380:CDS
ACTGGTGAAACTTGTTGCAC+AGG 0.522258 4_1:+80454984 None:intergenic
TTCATATTAGAACAAAACTT+AGG 0.532264 4_1:-80454127 Msa0576380:exon
GAAATCCAACATGCTACTAC+TGG 0.532832 4_1:+80454966 None:intergenic
ATGGGGAAACGAGTTGATGA+AGG 0.534464 4_1:+80454187 None:intergenic
AAGATCAACTTCCTGAGTCA+TGG 0.537926 4_1:-80455546 Msa0576380:CDS
TAATAGCTCTTCAGCTGGTG+GGG 0.544172 4_1:-80454530 Msa0576380:CDS
ACTTGTTGCACAGGATTTGG+AGG 0.545722 4_1:+80454993 None:intergenic
TTTCACCAGTAGTAGCATGT+TGG 0.549867 4_1:-80454971 Msa0576380:CDS
ATCAGGTGCAGGAGGGCGCA+TGG 0.552169 4_1:+80455675 None:intergenic
TGAACAACAGAAGCATTTGA+AGG 0.553398 4_1:+80455120 None:intergenic
TATATTCACCTTTCCAAATG+GGG 0.558912 4_1:+80454170 None:intergenic
AACTTTGATAGATGCTTCTG+TGG 0.560023 4_1:+80455900 None:intergenic
ATCTATCAAAGTTTCTACAA+AGG 0.563949 4_1:-80455890 Msa0576380:five_prime_UTR
ACTCGTTTCCCCATTTGGAA+AGG 0.564282 4_1:-80454178 Msa0576380:CDS
CTGGAAACCCTAACAACTGG+TGG 0.576344 4_1:-80455702 Msa0576380:CDS
AGAGGAAGAGAAGATGCATG+AGG 0.580543 4_1:+80455589 None:intergenic
TGATCAGAAGATAGATCTGA+TGG 0.584255 4_1:+80454913 None:intergenic
AGGAGGTGACTCCATGACTC+AGG 0.588341 4_1:+80455535 None:intergenic
TGTAATAGCTCTTCAGCTGG+TGG 0.590012 4_1:-80454532 Msa0576380:CDS
TGATGATGATGATCAGGTGC+AGG 0.595942 4_1:+80455664 None:intergenic
AAGTGTAATAGCTCTTCAGC+TGG 0.599130 4_1:-80454535 Msa0576380:CDS
TACTTGGTCTCAAATAGTGT+CGG 0.599741 4_1:-80455028 Msa0576380:CDS
TATTAATTAATTACATGAGG+AGG 0.601019 4_1:+80455518 None:intergenic
GGTTGGTTAAGAAGCTATAG+GGG 0.607374 4_1:+80455921 None:intergenic
GACACACTTAATATTAATCG+GGG 0.616970 4_1:+80453987 None:intergenic
TATAACTAGAAAGAGATCAA+AGG 0.620983 4_1:-80455834 Msa0576380:five_prime_UTR
TGGGGAAACGAGTTGATGAA+GGG 0.629472 4_1:+80454188 None:intergenic
TGGGGCATTGAAGAAAGCTA+GGG 0.639392 4_1:-80454512 Msa0576380:CDS
CACACTTAATATTAATCGGG+GGG 0.648993 4_1:+80453989 None:intergenic
TGATGATGATCAGGTGCAGG+AGG 0.659045 4_1:+80455667 None:intergenic
ACACACTTAATATTAATCGG+GGG 0.663608 4_1:+80453988 None:intergenic
TCAGAAGATAGATCTGATGG+TGG 0.669438 4_1:+80454916 None:intergenic
TGTGGTGTATGAAGAAGAGA+AGG 0.672441 4_1:-80455163 Msa0576380:CDS
ACAACAGAAGCATTTGAAGG+AGG 0.691723 4_1:+80455123 None:intergenic
CAGGATTTGGAGGAAGACGA+AGG 0.695718 4_1:+80455003 None:intergenic
GTTGGTTAAGAAGCTATAGG+GGG 0.701910 4_1:+80455922 None:intergenic
GATGATGATCAGGTGCAGGA+GGG 0.703453 4_1:+80455668 None:intergenic
TGGAAAATATAGGAGTGGTG+TGG 0.714324 4_1:-80455181 Msa0576380:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAGTTAATAATAATCA+TGG + chr4_1:80454019-80454038 None:intergenic 10.0%
!! ATCATATAGATAAAAAAAAA+TGG - chr4_1:80455317-80455336 Msa0576380:intron 10.0%
!!! ATTTTAGTTAATAATAATCA+TGG + chr4_1:80454019-80454038 None:intergenic 10.0%
!! ATCATATAGATAAAAAAAAA+TGG - chr4_1:80455317-80455336 Msa0576380:intron 10.0%
!! AAAAAAAGTTAAATTATGGA+CGG + chr4_1:80454700-80454719 None:intergenic 15.0%
!!! CAAAAATTATATATCCTTTT+TGG - chr4_1:80455380-80455399 Msa0576380:intron 15.0%
!!! TGTTTTTTCAAAGTTATTAT+TGG + chr4_1:80455929-80455948 None:intergenic 15.0%
!!! AACTTTGAAAAAACAAATTA+AGG - chr4_1:80455934-80455953 Msa0576380:five_prime_UTR 15.0%
!! AAAAAAAGTTAAATTATGGA+CGG + chr4_1:80454700-80454719 None:intergenic 15.0%
!!! CAAAAATTATATATCCTTTT+TGG - chr4_1:80455380-80455399 Msa0576380:intron 15.0%
!!! TGTTTTTTCAAAGTTATTAT+TGG + chr4_1:80455929-80455948 None:intergenic 15.0%
!!! AACTTTGAAAAAACAAATTA+AGG - chr4_1:80455934-80455953 Msa0576380:five_prime_UTR 15.0%
!! TATTAATTAATTACATGAGG+AGG + chr4_1:80454489-80454508 None:intergenic 20.0%
!! GTGCAAAAAAAGTTAAATTA+TGG + chr4_1:80454704-80454723 None:intergenic 20.0%
!! ACTATGATTTGATTATTTGA+AGG - chr4_1:80454745-80454764 Msa0576380:intron 20.0%
!!! GTCTTAATTTTTGATGATTT+TGG - chr4_1:80454803-80454822 Msa0576380:intron 20.0%
!!! TTGATGATTTTGGAAAATAT+AGG - chr4_1:80454813-80454832 Msa0576380:intron 20.0%
!! TTTGTTAAAAAGAGATTTCA+AGG + chr4_1:80455590-80455609 None:intergenic 20.0%
!! TTCATATTAGAACAAAACTT+AGG - chr4_1:80455877-80455896 Msa0576380:five_prime_UTR 20.0%
!!! TTTTCAAAGTTATTATTGGA+TGG + chr4_1:80455925-80455944 None:intergenic 20.0%
!! TATTAATTAATTACATGAGG+AGG + chr4_1:80454489-80454508 None:intergenic 20.0%
!! GTGCAAAAAAAGTTAAATTA+TGG + chr4_1:80454704-80454723 None:intergenic 20.0%
!! ACTATGATTTGATTATTTGA+AGG - chr4_1:80454745-80454764 Msa0576380:intron 20.0%
!!! GTCTTAATTTTTGATGATTT+TGG - chr4_1:80454803-80454822 Msa0576380:intron 20.0%
!!! TTGATGATTTTGGAAAATAT+AGG - chr4_1:80454813-80454832 Msa0576380:intron 20.0%
!! TTTGTTAAAAAGAGATTTCA+AGG + chr4_1:80455590-80455609 None:intergenic 20.0%
!! TTCATATTAGAACAAAACTT+AGG - chr4_1:80455877-80455896 Msa0576380:five_prime_UTR 20.0%
!!! TTTTCAAAGTTATTATTGGA+TGG + chr4_1:80455925-80455944 None:intergenic 20.0%
! ATCTATCAAAGTTTCTACAA+AGG - chr4_1:80454114-80454133 Msa0576380:three_prime_UTR 25.0%
! TCTATCAAAGTTTCTACAAA+GGG - chr4_1:80454115-80454134 Msa0576380:three_prime_UTR 25.0%
! TATAACTAGAAAGAGATCAA+AGG - chr4_1:80454170-80454189 Msa0576380:CDS 25.0%
!! AAGAAAAAAGGTATGAATAG+AGG - chr4_1:80454241-80454260 Msa0576380:intron 25.0%
! GGAGTAAGAAAATTAGAATT+AGG + chr4_1:80454391-80454410 None:intergenic 25.0%
! GGGTATTAATTAATTACATG+AGG + chr4_1:80454492-80454511 None:intergenic 25.0%
!!! AACTTTTTTTGCACACTTAA+TGG - chr4_1:80454710-80454729 Msa0576380:intron 25.0%
! TATGGAAATGAAGAATTTCA+TGG - chr4_1:80454941-80454960 Msa0576380:CDS 25.0%
! AGTAAGTTTCAATTGACAAT+TGG + chr4_1:80455235-80455254 None:intergenic 25.0%
!!! TCCAACTTTTTTTTGGAAAA+CGG - chr4_1:80455276-80455295 Msa0576380:intron 25.0%
! AACACAGAAATTAACCAAAA+AGG + chr4_1:80455397-80455416 None:intergenic 25.0%
! TTAAAAAGAGATTTCAAGGT+TGG + chr4_1:80455586-80455605 None:intergenic 25.0%
! ATATATTCACCTTTCCAAAT+GGG + chr4_1:80455838-80455857 None:intergenic 25.0%
! TATATATTCACCTTTCCAAA+TGG + chr4_1:80455839-80455858 None:intergenic 25.0%
! ATCTATCAAAGTTTCTACAA+AGG - chr4_1:80454114-80454133 Msa0576380:three_prime_UTR 25.0%
! TCTATCAAAGTTTCTACAAA+GGG - chr4_1:80454115-80454134 Msa0576380:three_prime_UTR 25.0%
! TATAACTAGAAAGAGATCAA+AGG - chr4_1:80454170-80454189 Msa0576380:CDS 25.0%
!! AAGAAAAAAGGTATGAATAG+AGG - chr4_1:80454241-80454260 Msa0576380:intron 25.0%
! GGAGTAAGAAAATTAGAATT+AGG + chr4_1:80454391-80454410 None:intergenic 25.0%
! GGGTATTAATTAATTACATG+AGG + chr4_1:80454492-80454511 None:intergenic 25.0%
!!! AACTTTTTTTGCACACTTAA+TGG - chr4_1:80454710-80454729 Msa0576380:intron 25.0%
! TATGGAAATGAAGAATTTCA+TGG - chr4_1:80454941-80454960 Msa0576380:CDS 25.0%
! AGTAAGTTTCAATTGACAAT+TGG + chr4_1:80455235-80455254 None:intergenic 25.0%
!!! TCCAACTTTTTTTTGGAAAA+CGG - chr4_1:80455276-80455295 Msa0576380:intron 25.0%
! AACACAGAAATTAACCAAAA+AGG + chr4_1:80455397-80455416 None:intergenic 25.0%
! TTAAAAAGAGATTTCAAGGT+TGG + chr4_1:80455586-80455605 None:intergenic 25.0%
! ATATATTCACCTTTCCAAAT+GGG + chr4_1:80455838-80455857 None:intergenic 25.0%
! TATATATTCACCTTTCCAAA+TGG + chr4_1:80455839-80455858 None:intergenic 25.0%
CTTTCTTGATGCTTCAAATA+GGG + chr4_1:80454145-80454164 None:intergenic 30.0%
TCTTTCTTGATGCTTCAAAT+AGG + chr4_1:80454146-80454165 None:intergenic 30.0%
TCTTGTCTTTGTAGAATCTA+GGG - chr4_1:80454210-80454229 Msa0576380:CDS 30.0%
AGGGAAATTGAGAAGAAAAA+AGG - chr4_1:80454229-80454248 Msa0576380:intron 30.0%
ATTAGAATTAGGAGTAGTAG+TGG + chr4_1:80454380-80454399 None:intergenic 30.0%
! TCTTGATTTTCATTCCAAGA+AGG + chr4_1:80454442-80454461 None:intergenic 30.0%
AGTGTTAGAGAAACAAAAAC+TGG + chr4_1:80454637-80454656 None:intergenic 30.0%
!! GATTTTGGAAAATATAGGAG+TGG - chr4_1:80454818-80454837 Msa0576380:intron 30.0%
! ATTTGAGACCAAGTAGAATT+TGG + chr4_1:80454971-80454990 None:intergenic 30.0%
GGATTTCTCTAACACTAAAA+CGG - chr4_1:80455054-80455073 Msa0576380:CDS 30.0%
!!! TTTGTCCTCCAACTTTTTTT+TGG - chr4_1:80455269-80455288 Msa0576380:intron 30.0%
! GTTTTCCAAAAAAAAGTTGG+AGG + chr4_1:80455277-80455296 None:intergenic 30.0%
! ACCGTTTTCCAAAAAAAAGT+TGG + chr4_1:80455280-80455299 None:intergenic 30.0%
! TTGATCTTCTCTTCATTTTC+AGG - chr4_1:80455760-80455779 Msa0576380:exon 30.0%
TATATTCACCTTTCCAAATG+GGG + chr4_1:80455837-80455856 None:intergenic 30.0%
ACACACTTAATATTAATCGG+GGG + chr4_1:80456019-80456038 None:intergenic 30.0%
GACACACTTAATATTAATCG+GGG + chr4_1:80456020-80456039 None:intergenic 30.0%
CCGATTAATATTAAGTGTGT+CGG - chr4_1:80456019-80456038 Msa0576380:five_prime_UTR 30.0%
CTTTCTTGATGCTTCAAATA+GGG + chr4_1:80454145-80454164 None:intergenic 30.0%
TCTTTCTTGATGCTTCAAAT+AGG + chr4_1:80454146-80454165 None:intergenic 30.0%
TCTTGTCTTTGTAGAATCTA+GGG - chr4_1:80454210-80454229 Msa0576380:CDS 30.0%
AGGGAAATTGAGAAGAAAAA+AGG - chr4_1:80454229-80454248 Msa0576380:intron 30.0%
ATTAGAATTAGGAGTAGTAG+TGG + chr4_1:80454380-80454399 None:intergenic 30.0%
! TCTTGATTTTCATTCCAAGA+AGG + chr4_1:80454442-80454461 None:intergenic 30.0%
AGTGTTAGAGAAACAAAAAC+TGG + chr4_1:80454637-80454656 None:intergenic 30.0%
!! GATTTTGGAAAATATAGGAG+TGG - chr4_1:80454818-80454837 Msa0576380:intron 30.0%
! ATTTGAGACCAAGTAGAATT+TGG + chr4_1:80454971-80454990 None:intergenic 30.0%
GGATTTCTCTAACACTAAAA+CGG - chr4_1:80455054-80455073 Msa0576380:CDS 30.0%
!!! TTTGTCCTCCAACTTTTTTT+TGG - chr4_1:80455269-80455288 Msa0576380:intron 30.0%
! GTTTTCCAAAAAAAAGTTGG+AGG + chr4_1:80455277-80455296 None:intergenic 30.0%
! ACCGTTTTCCAAAAAAAAGT+TGG + chr4_1:80455280-80455299 None:intergenic 30.0%
! TTGATCTTCTCTTCATTTTC+AGG - chr4_1:80455760-80455779 Msa0576380:exon 30.0%
TATATTCACCTTTCCAAATG+GGG + chr4_1:80455837-80455856 None:intergenic 30.0%
ACACACTTAATATTAATCGG+GGG + chr4_1:80456019-80456038 None:intergenic 30.0%
GACACACTTAATATTAATCG+GGG + chr4_1:80456020-80456039 None:intergenic 30.0%
! TGGTTGGTTAAGAAGCTATA+GGG + chr4_1:80454087-80454106 None:intergenic 35.0%
AACTTTGATAGATGCTTCTG+TGG + chr4_1:80454107-80454126 None:intergenic 35.0%
CTCTTGTCTTTGTAGAATCT+AGG - chr4_1:80454209-80454228 Msa0576380:CDS 35.0%
!! TTAGGTTGATGATGATGATC+AGG + chr4_1:80454349-80454368 None:intergenic 35.0%
! GTAGTAGTGGAGAAGAAATT+AGG + chr4_1:80454367-80454386 None:intergenic 35.0%
! TTTTCATTCCAAGAAGGAAG+AGG + chr4_1:80454436-80454455 None:intergenic 35.0%
GAGTGATTGAGAAAGAGAAA+GGG + chr4_1:80454512-80454531 None:intergenic 35.0%
TGAGTGATTGAGAAAGAGAA+AGG + chr4_1:80454513-80454532 None:intergenic 35.0%
!! TGAACAACAGAAGCATTTGA+AGG + chr4_1:80454887-80454906 None:intergenic 35.0%
TCAGTGAACAACTATGTCTA+TGG - chr4_1:80454923-80454942 Msa0576380:CDS 35.0%
ATGGAACACCAAATTCTACT+TGG - chr4_1:80454960-80454979 Msa0576380:CDS 35.0%
TACTTGGTCTCAAATAGTGT+CGG - chr4_1:80454976-80454995 Msa0576380:CDS 35.0%
TGATCAGAAGATAGATCTGA+TGG + chr4_1:80455094-80455113 None:intergenic 35.0%
ATCAGATCTATCTTCTGATC+AGG - chr4_1:80455093-80455112 Msa0576380:CDS 35.0%
TACAACTCAGTCTACATTCA+AGG - chr4_1:80455525-80455544 Msa0576380:intron 35.0%
ACACACACAAACATCATATG+TGG - chr4_1:80455613-80455632 Msa0576380:CDS 35.0%
CACACACAAACATCATATGT+GGG - chr4_1:80455614-80455633 Msa0576380:CDS 35.0%
!!! CTTCTCTTCATTTTCAGGTT+AGG - chr4_1:80455765-80455784 Msa0576380:exon 35.0%
!!! TCTTCATTTTCAGGTTAGGA+AGG - chr4_1:80455769-80455788 Msa0576380:exon 35.0%
CACACTTAATATTAATCGGG+GGG + chr4_1:80456018-80456037 None:intergenic 35.0%
! TGGTTGGTTAAGAAGCTATA+GGG + chr4_1:80454087-80454106 None:intergenic 35.0%
AACTTTGATAGATGCTTCTG+TGG + chr4_1:80454107-80454126 None:intergenic 35.0%
CTCTTGTCTTTGTAGAATCT+AGG - chr4_1:80454209-80454228 Msa0576380:CDS 35.0%
!! TTAGGTTGATGATGATGATC+AGG + chr4_1:80454349-80454368 None:intergenic 35.0%
! GTAGTAGTGGAGAAGAAATT+AGG + chr4_1:80454367-80454386 None:intergenic 35.0%
! TTTTCATTCCAAGAAGGAAG+AGG + chr4_1:80454436-80454455 None:intergenic 35.0%
GAGTGATTGAGAAAGAGAAA+GGG + chr4_1:80454512-80454531 None:intergenic 35.0%
TGAGTGATTGAGAAAGAGAA+AGG + chr4_1:80454513-80454532 None:intergenic 35.0%
!! TGAACAACAGAAGCATTTGA+AGG + chr4_1:80454887-80454906 None:intergenic 35.0%
TCAGTGAACAACTATGTCTA+TGG - chr4_1:80454923-80454942 Msa0576380:CDS 35.0%
ATGGAACACCAAATTCTACT+TGG - chr4_1:80454960-80454979 Msa0576380:CDS 35.0%
TACTTGGTCTCAAATAGTGT+CGG - chr4_1:80454976-80454995 Msa0576380:CDS 35.0%
TGATCAGAAGATAGATCTGA+TGG + chr4_1:80455094-80455113 None:intergenic 35.0%
ATCAGATCTATCTTCTGATC+AGG - chr4_1:80455093-80455112 Msa0576380:CDS 35.0%
TACAACTCAGTCTACATTCA+AGG - chr4_1:80455525-80455544 Msa0576380:intron 35.0%
ACACACACAAACATCATATG+TGG - chr4_1:80455613-80455632 Msa0576380:CDS 35.0%
CACACACAAACATCATATGT+GGG - chr4_1:80455614-80455633 Msa0576380:CDS 35.0%
!!! CTTCTCTTCATTTTCAGGTT+AGG - chr4_1:80455765-80455784 Msa0576380:exon 35.0%
!!! TCTTCATTTTCAGGTTAGGA+AGG - chr4_1:80455769-80455788 Msa0576380:exon 35.0%
CACACTTAATATTAATCGGG+GGG + chr4_1:80456018-80456037 None:intergenic 35.0%
GAAGGAACACTATTGTTGCT+TGG + chr4_1:80454063-80454082 None:intergenic 40.0%
GTTGGTTAAGAAGCTATAGG+GGG + chr4_1:80454085-80454104 None:intergenic 40.0%
! GGTTGGTTAAGAAGCTATAG+GGG + chr4_1:80454086-80454105 None:intergenic 40.0%
! GTGGTTGGTTAAGAAGCTAT+AGG + chr4_1:80454088-80454107 None:intergenic 40.0%
TTGATAGATGCTTCTGTGGT+TGG + chr4_1:80454103-80454122 None:intergenic 40.0%
AGAGAAGATGCATGAGGATA+TGG + chr4_1:80454412-80454431 None:intergenic 40.0%
AAGATCAACTTCCTGAGTCA+TGG - chr4_1:80454458-80454477 Msa0576380:intron 40.0%
! AAGCTGAGTTGAACTATCGA+CGG - chr4_1:80454581-80454600 Msa0576380:intron 40.0%
CTATCGACGGAAATTAGTGA+TGG - chr4_1:80454594-80454613 Msa0576380:intron 40.0%
TGGAAAATATAGGAGTGGTG+TGG - chr4_1:80454823-80454842 Msa0576380:intron 40.0%
TGTGGTGTATGAAGAAGAGA+AGG - chr4_1:80454841-80454860 Msa0576380:intron 40.0%
!! ACAACAGAAGCATTTGAAGG+AGG + chr4_1:80454884-80454903 None:intergenic 40.0%
!! GAAACTTGTTGCACAGGATT+TGG + chr4_1:80455017-80455036 None:intergenic 40.0%
TTTCACCAGTAGTAGCATGT+TGG - chr4_1:80455033-80455052 Msa0576380:CDS 40.0%
GAAATCCAACATGCTACTAC+TGG + chr4_1:80455041-80455060 None:intergenic 40.0%
TCAGAAGATAGATCTGATGG+TGG + chr4_1:80455091-80455110 None:intergenic 40.0%
AAGTGTAATAGCTCTTCAGC+TGG - chr4_1:80455469-80455488 Msa0576380:intron 40.0%
GCTGTAACTCTTGTCTTTCT+TGG - chr4_1:80455636-80455655 Msa0576380:CDS 40.0%
GAAGGAACACTATTGTTGCT+TGG + chr4_1:80454063-80454082 None:intergenic 40.0%
GTTGGTTAAGAAGCTATAGG+GGG + chr4_1:80454085-80454104 None:intergenic 40.0%
! GGTTGGTTAAGAAGCTATAG+GGG + chr4_1:80454086-80454105 None:intergenic 40.0%
! GTGGTTGGTTAAGAAGCTAT+AGG + chr4_1:80454088-80454107 None:intergenic 40.0%
TTGATAGATGCTTCTGTGGT+TGG + chr4_1:80454103-80454122 None:intergenic 40.0%
AGAGAAGATGCATGAGGATA+TGG + chr4_1:80454412-80454431 None:intergenic 40.0%
AAGATCAACTTCCTGAGTCA+TGG - chr4_1:80454458-80454477 Msa0576380:intron 40.0%
! AAGCTGAGTTGAACTATCGA+CGG - chr4_1:80454581-80454600 Msa0576380:intron 40.0%
CTATCGACGGAAATTAGTGA+TGG - chr4_1:80454594-80454613 Msa0576380:intron 40.0%
TGGAAAATATAGGAGTGGTG+TGG - chr4_1:80454823-80454842 Msa0576380:intron 40.0%
TGTGGTGTATGAAGAAGAGA+AGG - chr4_1:80454841-80454860 Msa0576380:intron 40.0%
!! ACAACAGAAGCATTTGAAGG+AGG + chr4_1:80454884-80454903 None:intergenic 40.0%
!! GAAACTTGTTGCACAGGATT+TGG + chr4_1:80455017-80455036 None:intergenic 40.0%
TTTCACCAGTAGTAGCATGT+TGG - chr4_1:80455033-80455052 Msa0576380:CDS 40.0%
GAAATCCAACATGCTACTAC+TGG + chr4_1:80455041-80455060 None:intergenic 40.0%
TCAGAAGATAGATCTGATGG+TGG + chr4_1:80455091-80455110 None:intergenic 40.0%
AAGTGTAATAGCTCTTCAGC+TGG - chr4_1:80455469-80455488 Msa0576380:intron 40.0%
GCTGTAACTCTTGTCTTTCT+TGG - chr4_1:80455636-80455655 Msa0576380:CDS 40.0%
GTTAAGAAGCTATAGGGGGA+AGG + chr4_1:80454081-80454100 None:intergenic 45.0%
CTCACCAGTGCAACAAATGA+TGG - chr4_1:80454279-80454298 Msa0576380:intron 45.0%
TGGTGTTCCACCAGTTGTTA+GGG + chr4_1:80454312-80454331 None:intergenic 45.0%
ATGGTGTTCCACCAGTTGTT+AGG + chr4_1:80454313-80454332 None:intergenic 45.0%
! TGATGATGATGATCAGGTGC+AGG + chr4_1:80454343-80454362 None:intergenic 45.0%
AGAGGAAGAGAAGATGCATG+AGG + chr4_1:80454418-80454437 None:intergenic 45.0%
CTTCTCTTCCTCTTCCTTCT+TGG - chr4_1:80454425-80454444 Msa0576380:intron 45.0%
! ACTTGTTGCACAGGATTTGG+AGG + chr4_1:80455014-80455033 None:intergenic 45.0%
!! ACTGGTGAAACTTGTTGCAC+AGG + chr4_1:80455023-80455042 None:intergenic 45.0%
TGTAATAGCTCTTCAGCTGG+TGG - chr4_1:80455472-80455491 Msa0576380:intron 45.0%
GTAATAGCTCTTCAGCTGGT+GGG - chr4_1:80455473-80455492 Msa0576380:intron 45.0%
TAATAGCTCTTCAGCTGGTG+GGG - chr4_1:80455474-80455493 Msa0576380:intron 45.0%
!! TGGGGCATTGAAGAAAGCTA+GGG - chr4_1:80455492-80455511 Msa0576380:intron 45.0%
CAGGTTAGGAAGGAGAAGTT+AGG - chr4_1:80455779-80455798 Msa0576380:five_prime_UTR 45.0%
!! TGGGGAAACGAGTTGATGAA+GGG + chr4_1:80455819-80455838 None:intergenic 45.0%
!! ATGGGGAAACGAGTTGATGA+AGG + chr4_1:80455820-80455839 None:intergenic 45.0%
CATCAACTCGTTTCCCCATT+TGG - chr4_1:80455821-80455840 Msa0576380:five_prime_UTR 45.0%
ACTCGTTTCCCCATTTGGAA+AGG - chr4_1:80455826-80455845 Msa0576380:five_prime_UTR 45.0%
GTTAAGAAGCTATAGGGGGA+AGG + chr4_1:80454081-80454100 None:intergenic 45.0%
CTCACCAGTGCAACAAATGA+TGG - chr4_1:80454279-80454298 Msa0576380:intron 45.0%
TGGTGTTCCACCAGTTGTTA+GGG + chr4_1:80454312-80454331 None:intergenic 45.0%
ATGGTGTTCCACCAGTTGTT+AGG + chr4_1:80454313-80454332 None:intergenic 45.0%
! TGATGATGATGATCAGGTGC+AGG + chr4_1:80454343-80454362 None:intergenic 45.0%
AGAGGAAGAGAAGATGCATG+AGG + chr4_1:80454418-80454437 None:intergenic 45.0%
CTTCTCTTCCTCTTCCTTCT+TGG - chr4_1:80454425-80454444 Msa0576380:intron 45.0%
! ACTTGTTGCACAGGATTTGG+AGG + chr4_1:80455014-80455033 None:intergenic 45.0%
!! ACTGGTGAAACTTGTTGCAC+AGG + chr4_1:80455023-80455042 None:intergenic 45.0%
TGTAATAGCTCTTCAGCTGG+TGG - chr4_1:80455472-80455491 Msa0576380:intron 45.0%
GTAATAGCTCTTCAGCTGGT+GGG - chr4_1:80455473-80455492 Msa0576380:intron 45.0%
TAATAGCTCTTCAGCTGGTG+GGG - chr4_1:80455474-80455493 Msa0576380:intron 45.0%
!! TGGGGCATTGAAGAAAGCTA+GGG - chr4_1:80455492-80455511 Msa0576380:intron 45.0%
CAGGTTAGGAAGGAGAAGTT+AGG - chr4_1:80455779-80455798 Msa0576380:five_prime_UTR 45.0%
!! TGGGGAAACGAGTTGATGAA+GGG + chr4_1:80455819-80455838 None:intergenic 45.0%
!! ATGGGGAAACGAGTTGATGA+AGG + chr4_1:80455820-80455839 None:intergenic 45.0%
CATCAACTCGTTTCCCCATT+TGG - chr4_1:80455821-80455840 Msa0576380:five_prime_UTR 45.0%
ACTCGTTTCCCCATTTGGAA+AGG - chr4_1:80455826-80455845 Msa0576380:five_prime_UTR 45.0%
!!! AACTTTATATTTTTTTTTAA+TGG - chr4_1:80454556-80454575 Msa0576380:intron 5.0%
!! TAATATGAATTTAATAAATT+AGG + chr4_1:80455866-80455885 None:intergenic 5.0%
!!! AACTTTATATTTTTTTTTAA+TGG - chr4_1:80454556-80454575 Msa0576380:intron 5.0%
!! TAATATGAATTTAATAAATT+AGG + chr4_1:80455866-80455885 None:intergenic 5.0%
! CCAGCCATCATTTGTTGCAC+TGG + chr4_1:80454286-80454305 None:intergenic 50.0%
CCAGTGCAACAAATGATGGC+TGG - chr4_1:80454283-80454302 Msa0576380:intron 50.0%
TGGCTGGAAACCCTAACAAC+TGG - chr4_1:80454299-80454318 Msa0576380:intron 50.0%
CTGGAAACCCTAACAACTGG+TGG - chr4_1:80454302-80454321 Msa0576380:intron 50.0%
GATGATGATCAGGTGCAGGA+GGG + chr4_1:80454339-80454358 None:intergenic 50.0%
! TGATGATGATCAGGTGCAGG+AGG + chr4_1:80454340-80454359 None:intergenic 50.0%
! AGGAGGCTGACTTAGCATAC+TGG + chr4_1:80454867-80454886 None:intergenic 50.0%
CAGGATTTGGAGGAAGACGA+AGG + chr4_1:80455004-80455023 None:intergenic 50.0%
!! GTGGGGCATTGAAGAAAGCT+AGG - chr4_1:80455491-80455510 Msa0576380:intron 50.0%
! CCAGCCATCATTTGTTGCAC+TGG + chr4_1:80454286-80454305 None:intergenic 50.0%
CCAGTGCAACAAATGATGGC+TGG - chr4_1:80454283-80454302 Msa0576380:intron 50.0%
TGGCTGGAAACCCTAACAAC+TGG - chr4_1:80454299-80454318 Msa0576380:intron 50.0%
CTGGAAACCCTAACAACTGG+TGG - chr4_1:80454302-80454321 Msa0576380:intron 50.0%
GATGATGATCAGGTGCAGGA+GGG + chr4_1:80454339-80454358 None:intergenic 50.0%
! TGATGATGATCAGGTGCAGG+AGG + chr4_1:80454340-80454359 None:intergenic 50.0%
! AGGAGGCTGACTTAGCATAC+TGG + chr4_1:80454867-80454886 None:intergenic 50.0%
CAGGATTTGGAGGAAGACGA+AGG + chr4_1:80455004-80455023 None:intergenic 50.0%
!! GTGGGGCATTGAAGAAAGCT+AGG - chr4_1:80455491-80455510 Msa0576380:intron 50.0%
AGGAGGTGACTCCATGACTC+AGG + chr4_1:80454472-80454491 None:intergenic 55.0%
AGGAGGTGACTCCATGACTC+AGG + chr4_1:80454472-80454491 None:intergenic 55.0%
! ATCAGGTGCAGGAGGGCGCA+TGG + chr4_1:80454332-80454351 None:intergenic 65.0%
! ATCAGGTGCAGGAGGGCGCA+TGG + chr4_1:80454332-80454351 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 80453987 80456039 80453987 ID=Msa0576380;Name=Msa0576380
chr4_1 mRNA 80453987 80456039 80453987 ID=Msa0576380-mRNA-1;Parent=Msa0576380;Name=Msa0576380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=266|1|1|1|1|1|4|153|233
chr4_1 exon 80453987 80454244 80453987 ID=Msa0576380-mRNA-1:exon:28781;Parent=Msa0576380-mRNA-1
chr4_1 exon 80454480 80454554 80454480 ID=Msa0576380-mRNA-1:exon:28780;Parent=Msa0576380-mRNA-1
chr4_1 exon 80454912 80455191 80454912 ID=Msa0576380-mRNA-1:exon:28779;Parent=Msa0576380-mRNA-1
chr4_1 exon 80455532 80456039 80455532 ID=Msa0576380-mRNA-1:exon:28778;Parent=Msa0576380-mRNA-1
chr4_1 five_prime_UTR 80455774 80456039 80455774 ID=Msa0576380-mRNA-1:five_prime_utr;Parent=Msa0576380-mRNA-1
chr4_1 CDS 80455532 80455773 80455532 ID=Msa0576380-mRNA-1:cds;Parent=Msa0576380-mRNA-1
chr4_1 CDS 80454912 80455191 80454912 ID=Msa0576380-mRNA-1:cds;Parent=Msa0576380-mRNA-1
chr4_1 CDS 80454480 80454554 80454480 ID=Msa0576380-mRNA-1:cds;Parent=Msa0576380-mRNA-1
chr4_1 CDS 80454140 80454244 80454140 ID=Msa0576380-mRNA-1:cds;Parent=Msa0576380-mRNA-1
chr4_1 three_prime_UTR 80453987 80454139 80453987 ID=Msa0576380-mRNA-1:three_prime_utr;Parent=Msa0576380-mRNA-1
Gene Sequence

>Msa0576380

ATGAATAGAGGTGTTTATCTGCAAAGCTCACCAGTGCAACAAATGATGGCTGGAAACCCTAACAACTGGTGGAACACCATGCGCCCTCCTGCACCTGATCATCATCATCAACCTAATTTCTTCTCCACTACTACTCCTAATTCTAATTTTCTTACTCCATATCCTCATGCATCTTCTCTTCCTCTTCCTTCTTGGAATGAAAATCAAGATCAACTTCCTGAGTCATGGAGTCACCTCCTCATGAGTGGTGTGGTGTATGAAGAAGAGAAGGCCAGTATGCTAAGTCAGCCTCCTTCAAATGCTTCTGTTGTTCATGTCAAACAAGAAAGTTCAGTGAACAACTATGTCTATGGAAATGAAGAATTTCATGGAACACCAAATTCTACTTGGTCTCAAATAGTGTCGGTGCCTTCGTCTTCCTCCAAATCCTGTGCAACAAGTTTCACCAGTAGTAGCATGTTGGATTTCTCTAACACTAAAACGGATATAAGATCTCCACCATCAGATCTATCTTCTGATCAGTGTAATAGCTCTTCAGCTGGTGGGGCATTGAAGAAAGCTAGGGTTCAATCATCTACAACTCAGTCTACATTCAAGGTTAGGAAGGAGAAGTTAGGTGACAGAATTACTGCCCTTCATCAACTCGTTTCCCCATTTGGAAAGGTGAATATATACAAGCCTAATTTATTAAATTCATATTAG
Protein sequence

>Msa0576380

MNRGVYLQSSPVQQMMAGNPNNWWNTMRPPAPDHHHQPNFFSTTTPNSNFLTPYPHASSLPLPSWNENQDQLPESWSHLLMSGVVYEEEKASMLSQPPSNASVVHVKQESSVNNYVYGNEEFHGTPNSTWSQIVSVPSSSSKSCATSFTSSSMLDFSNTKTDIRSPPSDLSSDQCNSSSAGGALKKARVQSSTTQSTFKVRKEKLGDRITALHQLVSPFGKVNIYKPNLLNSY*