AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577550 MtrunA17_Chr8g0377621 80.169 237 13 3 1 205 1 235 1.62e-116 331
Msa0577550 MtrunA17_Chr3g0122221 55.785 242 58 6 1 205 1 230 1.01e-74 224
Msa0577550 MtrunA17_Chr2g0289841 53.672 177 50 2 57 201 56 232 3.67e-58 182
Msa0577550 MtrunA17_Chr3g0120921 50.538 186 60 1 48 201 40 225 1.02e-56 179
Msa0577550 MtrunA17_Chr4g0075711 53.757 173 48 2 61 201 54 226 1.13e-55 176
Msa0577550 MtrunA17_Chr7g0261611 37.696 191 82 5 43 200 32 218 1.43e-35 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577550 AT3G07880.1 50.267 187 61 1 49 203 53 239 4.41e-59 186
Msa0577550 AT1G12070.1 49.143 175 55 3 61 201 48 222 7.03e-47 154
Msa0577550 AT1G62450.1 45.143 175 62 2 61 201 48 222 5.65e-44 146

Find 80 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCAAGCTGTTCTTTA+AGG 0.103059 4_1:+81885066 None:intergenic
ACCAATGTTGTTTGGAAAAC+TGG 0.188780 4_1:-81884333 Msa0577550:CDS
TTTCATCCCCTTCTATTATA+TGG 0.215093 4_1:-81883699 Msa0577550:three_prime_UTR
TTAAATCTTGGATGCATCTT+AGG 0.217285 4_1:+81883606 None:intergenic
GCTACTTGGATGCTAGTTAT+AGG 0.254947 4_1:-81883843 Msa0577550:CDS
ATGAATTAAATTTCACATAA+AGG 0.300428 4_1:-81883658 Msa0577550:three_prime_UTR
GCAGACATTGTCAAATTTAG+AGG 0.309517 4_1:+81885275 None:intergenic
TTTAATTGTTATTATCTGTT+AGG 0.310142 4_1:-81883588 Msa0577550:three_prime_UTR
AGCATTGAAGGAAACATCTT+TGG 0.319587 4_1:+81885229 None:intergenic
GTCTCCAACAACATAGTTTC+TGG 0.320251 4_1:-81884366 Msa0577550:CDS
AAAAGGAACCATATAATAGA+AGG 0.328086 4_1:+81883691 None:intergenic
TTTCTCAAGCTGTTCTTTAA+GGG 0.328321 4_1:+81885067 None:intergenic
TCTTCCTCTTCATCTTCATC+TGG 0.347069 4_1:+81885131 None:intergenic
ATGATGAAAGCTTGAGGAAA+TGG 0.349670 4_1:-81884942 Msa0577550:CDS
AAATGGAAAGAGCAACTTCT+AGG 0.351067 4_1:-81884925 Msa0577550:CDS
TCAAATATACCAATGTTGTT+TGG 0.363873 4_1:-81884341 Msa0577550:CDS
GAATCAGCGGTGAATGGAAT+TGG 0.371922 4_1:+81884450 None:intergenic
GGTTTGAGATTCAGAAGAAT+TGG 0.376482 4_1:-81883822 Msa0577550:CDS
AGGCCTTCCTTGGCATATAA+TGG 0.379225 4_1:+81884485 None:intergenic
TGTTTCCTTCAATGCTCATT+TGG 0.380600 4_1:-81885222 Msa0577550:CDS
GGAAGAACCTAAGTTGGAAT+TGG 0.382333 4_1:-81885108 Msa0577550:CDS
TTCCTCCAAATGAGCATTGA+AGG 0.385347 4_1:+81885217 None:intergenic
GACTGAGGGGTGAAAAGAAA+TGG 0.401381 4_1:-81883560 Msa0577550:three_prime_UTR
AGGCTCGCTATCTTCACTTC+TGG 0.402038 4_1:+81884510 None:intergenic
CCTAAGTTGGAATTGGATCT+TGG 0.402975 4_1:-81885101 Msa0577550:CDS
CCAAGATCCAATTCCAACTT+AGG 0.415301 4_1:+81885101 None:intergenic
TGTTTATTAAACTAAGTTAG+TGG 0.416620 4_1:-81883758 Msa0577550:three_prime_UTR
ACTGAGGGGTGAAAAGAAAT+GGG 0.417413 4_1:-81883559 Msa0577550:three_prime_UTR
ATTACATTACCAAGGGATAT+AGG 0.417448 4_1:-81885310 Msa0577550:five_prime_UTR
CCTTGGTAATGTAATATGTA+TGG 0.425085 4_1:+81885318 None:intergenic
CCAATGTTGTTTGGAAAACT+GGG 0.429548 4_1:-81884332 Msa0577550:CDS
AGATAATAACAATTAAATCT+TGG 0.436258 4_1:+81883594 None:intergenic
GTTTGGAAAACTGGGGTTAG+AGG 0.436517 4_1:-81884324 Msa0577550:CDS
GAGGCCAGAAACTATGTTGT+TGG 0.443718 4_1:+81884362 None:intergenic
GGAAGAGGAAGAACCTAAGT+TGG 0.456835 4_1:-81885114 Msa0577550:CDS
ATAGTTGACACCAACCTTGT+TGG 0.461291 4_1:+81885175 None:intergenic
GAAAAGAAATGGGGTGGGAA+TGG 0.466205 4_1:-81883549 Msa0577550:three_prime_UTR
AGATTAGGTCAGGCCTTCCT+TGG 0.466881 4_1:+81884475 None:intergenic
AAACAACATTGGTATATTTG+AGG 0.467217 4_1:+81884343 None:intergenic
TCAAGGAAGGAAGCCAATAC+AGG 0.471561 4_1:-81884407 Msa0577550:CDS
TTCCTTCAATGCTCATTTGG+AGG 0.498763 4_1:-81885219 Msa0577550:CDS
TGATGATCGCAAATGCTACT+TGG 0.504667 4_1:-81883857 Msa0577550:CDS
AGATGAAGATGAAGAGGAAG+AGG 0.507523 4_1:-81885129 Msa0577550:CDS
GGAATTGGCAAGATTAGGTC+AGG 0.511505 4_1:+81884465 None:intergenic
AATCTGTCTCAGTGTGGTGT+AGG 0.514298 4_1:+81883799 None:intergenic
AAAGAGCATGTTCACTCTCA+AGG 0.517312 4_1:-81884424 Msa0577550:CDS
AAAGGAACCATATAATAGAA+GGG 0.530739 4_1:+81883692 None:intergenic
TGAATGGAATTGGCAAGATT+AGG 0.534268 4_1:+81884460 None:intergenic
AATCTTGGATGCATCTTAGG+TGG 0.541371 4_1:+81883609 None:intergenic
TGTGCCAGATGAAGATGAAG+AGG 0.547332 4_1:-81885135 Msa0577550:CDS
GTGGATGTGTCTGCTGTTGG+AGG 0.550509 4_1:-81884898 Msa0577550:intron
TCTAACTGAAACATAAATTG+AGG 0.554988 4_1:+81884290 None:intergenic
AGGGGTGAAAAGAAATGGGG+TGG 0.559682 4_1:-81883555 Msa0577550:three_prime_UTR
TATTTCTGTGCACATCCACA+TGG 0.562058 4_1:+81883523 None:intergenic
AATGTGGATGTGTCTGCTGT+TGG 0.567791 4_1:-81884901 Msa0577550:CDS
AGAGCAACTTCTAGGAAATG+TGG 0.570308 4_1:-81884917 Msa0577550:CDS
TTCCTTGGCATATAATGGTG+AGG 0.572917 4_1:+81884490 None:intergenic
TAAAGGATGATGAAAGCTTG+AGG 0.574330 4_1:-81884948 Msa0577550:intron
AGCCTCACCATTATATGCCA+AGG 0.579121 4_1:-81884492 Msa0577550:CDS
ATCTGTTAGGAGTGTGACTG+AGG 0.600412 4_1:-81883575 Msa0577550:three_prime_UTR
AGCATGTTCACTCTCAAGGA+AGG 0.602637 4_1:-81884420 Msa0577550:CDS
AACAAAGGAACCAACAAGGT+TGG 0.603340 4_1:-81885185 Msa0577550:CDS
GTTCTTTAAGGGAAAACTGA+GGG 0.605683 4_1:+81885078 None:intergenic
GGGTGGGAATGGACCCCATG+TGG 0.607426 4_1:-81883538 Msa0577550:three_prime_UTR
ATTTCTGTGCACATCCACAT+GGG 0.613728 4_1:+81883524 None:intergenic
TTGACAATGTCTGCTGCTGT+AGG 0.616083 4_1:-81885266 Msa0577550:exon
TCACCATTATATGCCAAGGA+AGG 0.618936 4_1:-81884488 Msa0577550:CDS
AAGGAACCATATAATAGAAG+GGG 0.620485 4_1:+81883693 None:intergenic
CATACATATTACATTACCAA+GGG 0.621345 4_1:-81885317 Msa0577550:five_prime_UTR
TATGTTGTTGGAGACAGTGA+AGG 0.635616 4_1:+81884374 None:intergenic
CCATACATATTACATTACCA+AGG 0.643408 4_1:-81885318 Msa0577550:five_prime_UTR
TCTGTTAGGAGTGTGACTGA+GGG 0.643817 4_1:-81883574 Msa0577550:three_prime_UTR
GGGGTGAAAAGAAATGGGGT+GGG 0.646062 4_1:-81883554 Msa0577550:three_prime_UTR
CAATGTTGTTTGGAAAACTG+GGG 0.646093 4_1:-81884331 Msa0577550:CDS
CTGAGGGGTGAAAAGAAATG+GGG 0.656530 4_1:-81883558 Msa0577550:three_prime_UTR
TGTTCTTTAAGGGAAAACTG+AGG 0.676471 4_1:+81885077 None:intergenic
TTTCTGTGCACATCCACATG+GGG 0.681268 4_1:+81883525 None:intergenic
AATTTCAATCTGTCTCAGTG+TGG 0.687125 4_1:+81883793 None:intergenic
CTGTTAGGAGTGTGACTGAG+GGG 0.699002 4_1:-81883573 Msa0577550:three_prime_UTR
TGTCAACTATGATGATGCTG+AGG 0.722211 4_1:-81885162 Msa0577550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTTTTTATTTTATT+TGG - chr4_1:81884973-81884992 Msa0577550:intron 0.0%
!!! TTAATTTTTTTTATTTTATT+TGG - chr4_1:81884973-81884992 Msa0577550:intron 0.0%
!!! AATTTTGTTTTGTATTTTAA+AGG - chr4_1:81883928-81883947 Msa0577550:intron 10.0%
!!! AATTTTGTTTTGTATTTTAA+AGG - chr4_1:81883928-81883947 Msa0577550:intron 10.0%
!! ATAAAAGAAGTCAATTAAAT+TGG + chr4_1:81884130-81884149 None:intergenic 15.0%
!! TTATGAATTTATTCAAATAG+TGG - chr4_1:81884673-81884692 Msa0577550:intron 15.0%
!! AATTCATTACAATACAAAAA+AGG + chr4_1:81885222-81885241 None:intergenic 15.0%
!! ATGAATTAAATTTCACATAA+AGG - chr4_1:81885235-81885254 Msa0577550:CDS 15.0%
!! AGATAATAACAATTAAATCT+TGG + chr4_1:81885302-81885321 None:intergenic 15.0%
!!! TTTAATTGTTATTATCTGTT+AGG - chr4_1:81885305-81885324 Msa0577550:five_prime_UTR 15.0%
!! ATAAAAGAAGTCAATTAAAT+TGG + chr4_1:81884130-81884149 None:intergenic 15.0%
!! TTATGAATTTATTCAAATAG+TGG - chr4_1:81884673-81884692 Msa0577550:intron 15.0%
!! AATTCATTACAATACAAAAA+AGG + chr4_1:81885222-81885241 None:intergenic 15.0%
!! ATGAATTAAATTTCACATAA+AGG - chr4_1:81885235-81885254 Msa0577550:CDS 15.0%
!! AGATAATAACAATTAAATCT+TGG + chr4_1:81885302-81885321 None:intergenic 15.0%
!!! TTTAATTGTTATTATCTGTT+AGG - chr4_1:81885305-81885324 Msa0577550:five_prime_UTR 15.0%
!!! TTTAATTGACTTCTTTTATC+AGG - chr4_1:81884131-81884150 Msa0577550:intron 20.0%
!! TGTTTATTAAACTAAGTTAG+TGG - chr4_1:81885135-81885154 Msa0577550:CDS 20.0%
!!! TTTAATTGACTTCTTTTATC+AGG - chr4_1:81884131-81884150 Msa0577550:intron 20.0%
!! TGTTTATTAAACTAAGTTAG+TGG - chr4_1:81885135-81885154 Msa0577550:CDS 20.0%
! CATACATATTACATTACCAA+GGG - chr4_1:81883576-81883595 Msa0577550:three_prime_UTR 25.0%
!!! AGTAAGGTTCTTTTTTGAAA+TGG - chr4_1:81883854-81883873 Msa0577550:CDS 25.0%
!!! GTAGTGTTTTTTTGATGAAT+TGG - chr4_1:81883904-81883923 Msa0577550:intron 25.0%
! AGAAGAAAAACAAAATCGAA+AGG + chr4_1:81884031-81884050 None:intergenic 25.0%
!!! CTTCTGAAACAATTCTTAAA+TGG - chr4_1:81884046-81884065 Msa0577550:intron 25.0%
! TCAATTAAATTGGAAGAAGA+GGG + chr4_1:81884120-81884139 None:intergenic 25.0%
! TGTTAATAAAAACAAGAACC+CGG + chr4_1:81884324-81884343 None:intergenic 25.0%
! AAACAACATTGGTATATTTG+AGG + chr4_1:81884553-81884572 None:intergenic 25.0%
! TCAAATATACCAATGTTGTT+TGG - chr4_1:81884552-81884571 Msa0577550:intron 25.0%
! TCTAACTGAAACATAAATTG+AGG + chr4_1:81884606-81884625 None:intergenic 25.0%
! TTTGAATTGTCTTAGTTATG+TGG - chr4_1:81884647-81884666 Msa0577550:intron 25.0%
! TCTTCCAATTCATATGTATA+TGG + chr4_1:81884743-81884762 None:intergenic 25.0%
! TGAAACTAACACATACTTAT+CGG - chr4_1:81885000-81885019 Msa0577550:intron 25.0%
! AAAGGAACCATATAATAGAA+GGG + chr4_1:81885204-81885223 None:intergenic 25.0%
! AAAAGGAACCATATAATAGA+AGG + chr4_1:81885205-81885224 None:intergenic 25.0%
! CATACATATTACATTACCAA+GGG - chr4_1:81883576-81883595 Msa0577550:three_prime_UTR 25.0%
!!! AGTAAGGTTCTTTTTTGAAA+TGG - chr4_1:81883854-81883873 Msa0577550:CDS 25.0%
!!! GTAGTGTTTTTTTGATGAAT+TGG - chr4_1:81883904-81883923 Msa0577550:intron 25.0%
! AGAAGAAAAACAAAATCGAA+AGG + chr4_1:81884031-81884050 None:intergenic 25.0%
!!! CTTCTGAAACAATTCTTAAA+TGG - chr4_1:81884046-81884065 Msa0577550:intron 25.0%
! TCAATTAAATTGGAAGAAGA+GGG + chr4_1:81884120-81884139 None:intergenic 25.0%
! TGTTAATAAAAACAAGAACC+CGG + chr4_1:81884324-81884343 None:intergenic 25.0%
! AAACAACATTGGTATATTTG+AGG + chr4_1:81884553-81884572 None:intergenic 25.0%
! TCAAATATACCAATGTTGTT+TGG - chr4_1:81884552-81884571 Msa0577550:intron 25.0%
! TCTAACTGAAACATAAATTG+AGG + chr4_1:81884606-81884625 None:intergenic 25.0%
! TTTGAATTGTCTTAGTTATG+TGG - chr4_1:81884647-81884666 Msa0577550:intron 25.0%
! TCTTCCAATTCATATGTATA+TGG + chr4_1:81884743-81884762 None:intergenic 25.0%
! TGAAACTAACACATACTTAT+CGG - chr4_1:81885000-81885019 Msa0577550:intron 25.0%
! AAAGGAACCATATAATAGAA+GGG + chr4_1:81885204-81885223 None:intergenic 25.0%
! AAAAGGAACCATATAATAGA+AGG + chr4_1:81885205-81885224 None:intergenic 25.0%
!! CCTTGGTAATGTAATATGTA+TGG + chr4_1:81883578-81883597 None:intergenic 30.0%
CCATACATATTACATTACCA+AGG - chr4_1:81883575-81883594 Msa0577550:three_prime_UTR 30.0%
ATTACATTACCAAGGGATAT+AGG - chr4_1:81883583-81883602 Msa0577550:three_prime_UTR 30.0%
TTTCTCAAGCTGTTCTTTAA+GGG + chr4_1:81883829-81883848 None:intergenic 30.0%
AGCTTGAGAAAGATAAAGTA+AGG - chr4_1:81883838-81883857 Msa0577550:CDS 30.0%
GTCAATTAAATTGGAAGAAG+AGG + chr4_1:81884121-81884140 None:intergenic 30.0%
! CTCTCATCTTTTACATTTAC+AGG - chr4_1:81884170-81884189 Msa0577550:intron 30.0%
! ATAAGTTCCCAACATTTTCT+TGG + chr4_1:81884705-81884724 None:intergenic 30.0%
GGAACCATATACATATGAAT+TGG - chr4_1:81884736-81884755 Msa0577550:intron 30.0%
! ATTTTGTAAGTTTCTTGCCT+TGG + chr4_1:81884816-81884835 None:intergenic 30.0%
TTTCATCCCCTTCTATTATA+TGG - chr4_1:81885194-81885213 Msa0577550:CDS 30.0%
AAGGAACCATATAATAGAAG+GGG + chr4_1:81885203-81885222 None:intergenic 30.0%
TTAAATCTTGGATGCATCTT+AGG + chr4_1:81885290-81885309 None:intergenic 30.0%
!! CCTTGGTAATGTAATATGTA+TGG + chr4_1:81883578-81883597 None:intergenic 30.0%
CCATACATATTACATTACCA+AGG - chr4_1:81883575-81883594 Msa0577550:three_prime_UTR 30.0%
ATTACATTACCAAGGGATAT+AGG - chr4_1:81883583-81883602 Msa0577550:three_prime_UTR 30.0%
TTTCTCAAGCTGTTCTTTAA+GGG + chr4_1:81883829-81883848 None:intergenic 30.0%
AGCTTGAGAAAGATAAAGTA+AGG - chr4_1:81883838-81883857 Msa0577550:CDS 30.0%
GTCAATTAAATTGGAAGAAG+AGG + chr4_1:81884121-81884140 None:intergenic 30.0%
! CTCTCATCTTTTACATTTAC+AGG - chr4_1:81884170-81884189 Msa0577550:intron 30.0%
! ATAAGTTCCCAACATTTTCT+TGG + chr4_1:81884705-81884724 None:intergenic 30.0%
GGAACCATATACATATGAAT+TGG - chr4_1:81884736-81884755 Msa0577550:intron 30.0%
! ATTTTGTAAGTTTCTTGCCT+TGG + chr4_1:81884816-81884835 None:intergenic 30.0%
TTTCATCCCCTTCTATTATA+TGG - chr4_1:81885194-81885213 Msa0577550:CDS 30.0%
AAGGAACCATATAATAGAAG+GGG + chr4_1:81885203-81885222 None:intergenic 30.0%
TTAAATCTTGGATGCATCTT+AGG + chr4_1:81885290-81885309 None:intergenic 30.0%
GCAGACATTGTCAAATTTAG+AGG + chr4_1:81883621-81883640 None:intergenic 35.0%
! AGCATTGAAGGAAACATCTT+TGG + chr4_1:81883667-81883686 None:intergenic 35.0%
TGTTTCCTTCAATGCTCATT+TGG - chr4_1:81883671-81883690 Msa0577550:three_prime_UTR 35.0%
CAAAAACAAAGGAACCAACA+AGG - chr4_1:81883704-81883723 Msa0577550:three_prime_UTR 35.0%
! GTTCTTTAAGGGAAAACTGA+GGG + chr4_1:81883818-81883837 None:intergenic 35.0%
! TGTTCTTTAAGGGAAAACTG+AGG + chr4_1:81883819-81883838 None:intergenic 35.0%
CTTTCTCAAGCTGTTCTTTA+AGG + chr4_1:81883830-81883849 None:intergenic 35.0%
TAAAGGATGATGAAAGCTTG+AGG - chr4_1:81883945-81883964 Msa0577550:intron 35.0%
ATGATGAAAGCTTGAGGAAA+TGG - chr4_1:81883951-81883970 Msa0577550:intron 35.0%
AAATGGAAAGAGCAACTTCT+AGG - chr4_1:81883968-81883987 Msa0577550:intron 35.0%
!!! TTTTTTGGCCGAAATTTGGT+TGG + chr4_1:81884237-81884256 None:intergenic 35.0%
!!! AGGATTTTTTGGCCGAAATT+TGG + chr4_1:81884241-81884260 None:intergenic 35.0%
!!! AAGACACGATCAGGATTTTT+TGG + chr4_1:81884252-81884271 None:intergenic 35.0%
AAAAAATCCTGATCGTGTCT+TGG - chr4_1:81884251-81884270 Msa0577550:intron 35.0%
TCTCTACATCACAACAAGAT+AGG + chr4_1:81884355-81884374 None:intergenic 35.0%
TGAATGGAATTGGCAAGATT+AGG + chr4_1:81884436-81884455 None:intergenic 35.0%
!! AGGAGAATTTTAGCCTGTAT+TGG + chr4_1:81884502-81884521 None:intergenic 35.0%
! ACCAATGTTGTTTGGAAAAC+TGG - chr4_1:81884560-81884579 Msa0577550:intron 35.0%
! CCAATGTTGTTTGGAAAACT+GGG - chr4_1:81884561-81884580 Msa0577550:intron 35.0%
! CAATGTTGTTTGGAAAACTG+GGG - chr4_1:81884562-81884581 Msa0577550:intron 35.0%
GAGAACACCAAGAAAATGTT+GGG - chr4_1:81884695-81884714 Msa0577550:intron 35.0%
GAAGAAGAAATTACTCCTTC+AGG - chr4_1:81884758-81884777 Msa0577550:intron 35.0%
TGTCAATGCTACACAAGTAA+CGG + chr4_1:81884870-81884889 None:intergenic 35.0%
! GGTTTGAGATTCAGAAGAAT+TGG - chr4_1:81885071-81885090 Msa0577550:CDS 35.0%
AATTTCAATCTGTCTCAGTG+TGG + chr4_1:81885103-81885122 None:intergenic 35.0%
GCAGACATTGTCAAATTTAG+AGG + chr4_1:81883621-81883640 None:intergenic 35.0%
! AGCATTGAAGGAAACATCTT+TGG + chr4_1:81883667-81883686 None:intergenic 35.0%
TGTTTCCTTCAATGCTCATT+TGG - chr4_1:81883671-81883690 Msa0577550:three_prime_UTR 35.0%
CAAAAACAAAGGAACCAACA+AGG - chr4_1:81883704-81883723 Msa0577550:three_prime_UTR 35.0%
! GTTCTTTAAGGGAAAACTGA+GGG + chr4_1:81883818-81883837 None:intergenic 35.0%
! TGTTCTTTAAGGGAAAACTG+AGG + chr4_1:81883819-81883838 None:intergenic 35.0%
CTTTCTCAAGCTGTTCTTTA+AGG + chr4_1:81883830-81883849 None:intergenic 35.0%
TAAAGGATGATGAAAGCTTG+AGG - chr4_1:81883945-81883964 Msa0577550:intron 35.0%
ATGATGAAAGCTTGAGGAAA+TGG - chr4_1:81883951-81883970 Msa0577550:intron 35.0%
AAATGGAAAGAGCAACTTCT+AGG - chr4_1:81883968-81883987 Msa0577550:intron 35.0%
!!! TTTTTTGGCCGAAATTTGGT+TGG + chr4_1:81884237-81884256 None:intergenic 35.0%
!!! AGGATTTTTTGGCCGAAATT+TGG + chr4_1:81884241-81884260 None:intergenic 35.0%
!!! AAGACACGATCAGGATTTTT+TGG + chr4_1:81884252-81884271 None:intergenic 35.0%
AAAAAATCCTGATCGTGTCT+TGG - chr4_1:81884251-81884270 Msa0577550:intron 35.0%
TCTCTACATCACAACAAGAT+AGG + chr4_1:81884355-81884374 None:intergenic 35.0%
TGAATGGAATTGGCAAGATT+AGG + chr4_1:81884436-81884455 None:intergenic 35.0%
!! AGGAGAATTTTAGCCTGTAT+TGG + chr4_1:81884502-81884521 None:intergenic 35.0%
! ACCAATGTTGTTTGGAAAAC+TGG - chr4_1:81884560-81884579 Msa0577550:intron 35.0%
! CCAATGTTGTTTGGAAAACT+GGG - chr4_1:81884561-81884580 Msa0577550:intron 35.0%
! CAATGTTGTTTGGAAAACTG+GGG - chr4_1:81884562-81884581 Msa0577550:intron 35.0%
GAGAACACCAAGAAAATGTT+GGG - chr4_1:81884695-81884714 Msa0577550:intron 35.0%
GAAGAAGAAATTACTCCTTC+AGG - chr4_1:81884758-81884777 Msa0577550:intron 35.0%
TGTCAATGCTACACAAGTAA+CGG + chr4_1:81884870-81884889 None:intergenic 35.0%
! GGTTTGAGATTCAGAAGAAT+TGG - chr4_1:81885071-81885090 Msa0577550:CDS 35.0%
AATTTCAATCTGTCTCAGTG+TGG + chr4_1:81885103-81885122 None:intergenic 35.0%
! GTTTTGCTTCCTATATCCCT+TGG + chr4_1:81883595-81883614 None:intergenic 40.0%
TTCCTTCAATGCTCATTTGG+AGG - chr4_1:81883674-81883693 Msa0577550:three_prime_UTR 40.0%
!! TTCCTCCAAATGAGCATTGA+AGG + chr4_1:81883679-81883698 None:intergenic 40.0%
! GAGGAAGAGCACAAAAACAA+AGG - chr4_1:81883693-81883712 Msa0577550:three_prime_UTR 40.0%
AACAAAGGAACCAACAAGGT+TGG - chr4_1:81883708-81883727 Msa0577550:three_prime_UTR 40.0%
ATAGTTGACACCAACCTTGT+TGG + chr4_1:81883721-81883740 None:intergenic 40.0%
! TGTCAACTATGATGATGCTG+AGG - chr4_1:81883731-81883750 Msa0577550:three_prime_UTR 40.0%
TCTTCCTCTTCATCTTCATC+TGG + chr4_1:81883765-81883784 None:intergenic 40.0%
AGATGAAGATGAAGAGGAAG+AGG - chr4_1:81883764-81883783 Msa0577550:three_prime_UTR 40.0%
! GGAAGAACCTAAGTTGGAAT+TGG - chr4_1:81883785-81883804 Msa0577550:three_prime_UTR 40.0%
CCAAGATCCAATTCCAACTT+AGG + chr4_1:81883795-81883814 None:intergenic 40.0%
! CCTAAGTTGGAATTGGATCT+TGG - chr4_1:81883792-81883811 Msa0577550:exon 40.0%
! AGAGCAACTTCTAGGAAATG+TGG - chr4_1:81883976-81883995 Msa0577550:intron 40.0%
CAGCATGTCCAACCAAATTT+CGG - chr4_1:81884226-81884245 Msa0577550:intron 40.0%
TCAGACAAATCTTGACCGTT+AGG + chr4_1:81884277-81884296 None:intergenic 40.0%
CGGTCAAGATTTGTCTGAAT+CGG - chr4_1:81884279-81884298 Msa0577550:intron 40.0%
CAAGATTTGTCTGAATCGGT+TGG - chr4_1:81884283-81884302 Msa0577550:intron 40.0%
TTCCTTGGCATATAATGGTG+AGG + chr4_1:81884406-81884425 None:intergenic 40.0%
TCACCATTATATGCCAAGGA+AGG - chr4_1:81884405-81884424 Msa0577550:CDS 40.0%
!!! TTTTTGGAATCAGCGGTGAA+TGG + chr4_1:81884452-81884471 None:intergenic 40.0%
!!! CATGCTCTTTTTGGAATCAG+CGG + chr4_1:81884459-81884478 None:intergenic 40.0%
! GAGAGTGAACATGCTCTTTT+TGG + chr4_1:81884468-81884487 None:intergenic 40.0%
! AAAGAGCATGTTCACTCTCA+AGG - chr4_1:81884469-81884488 Msa0577550:CDS 40.0%
TATGTTGTTGGAGACAGTGA+AGG + chr4_1:81884522-81884541 None:intergenic 40.0%
GTCTCCAACAACATAGTTTC+TGG - chr4_1:81884527-81884546 Msa0577550:intron 40.0%
! CCCAGTTTTCCAAACAACAT+TGG + chr4_1:81884564-81884583 None:intergenic 40.0%
GGAGAACACCAAGAAAATGT+TGG - chr4_1:81884694-81884713 Msa0577550:intron 40.0%
ATGTATATGGTTCCTGCTGT+GGG + chr4_1:81884730-81884749 None:intergenic 40.0%
TATGTATATGGTTCCTGCTG+TGG + chr4_1:81884731-81884750 None:intergenic 40.0%
TGCGATCATCATCTACAAAC+TGG + chr4_1:81885027-81885046 None:intergenic 40.0%
TGATGATCGCAAATGCTACT+TGG - chr4_1:81885036-81885055 Msa0577550:intron 40.0%
GCTACTTGGATGCTAGTTAT+AGG - chr4_1:81885050-81885069 Msa0577550:intron 40.0%
AATCTTGGATGCATCTTAGG+TGG + chr4_1:81885287-81885306 None:intergenic 40.0%
ACTGAGGGGTGAAAAGAAAT+GGG - chr4_1:81885334-81885353 Msa0577550:five_prime_UTR 40.0%
! GTTTTGCTTCCTATATCCCT+TGG + chr4_1:81883595-81883614 None:intergenic 40.0%
TTCCTTCAATGCTCATTTGG+AGG - chr4_1:81883674-81883693 Msa0577550:three_prime_UTR 40.0%
!! TTCCTCCAAATGAGCATTGA+AGG + chr4_1:81883679-81883698 None:intergenic 40.0%
! GAGGAAGAGCACAAAAACAA+AGG - chr4_1:81883693-81883712 Msa0577550:three_prime_UTR 40.0%
AACAAAGGAACCAACAAGGT+TGG - chr4_1:81883708-81883727 Msa0577550:three_prime_UTR 40.0%
ATAGTTGACACCAACCTTGT+TGG + chr4_1:81883721-81883740 None:intergenic 40.0%
! TGTCAACTATGATGATGCTG+AGG - chr4_1:81883731-81883750 Msa0577550:three_prime_UTR 40.0%
TCTTCCTCTTCATCTTCATC+TGG + chr4_1:81883765-81883784 None:intergenic 40.0%
AGATGAAGATGAAGAGGAAG+AGG - chr4_1:81883764-81883783 Msa0577550:three_prime_UTR 40.0%
! GGAAGAACCTAAGTTGGAAT+TGG - chr4_1:81883785-81883804 Msa0577550:three_prime_UTR 40.0%
CCAAGATCCAATTCCAACTT+AGG + chr4_1:81883795-81883814 None:intergenic 40.0%
! CCTAAGTTGGAATTGGATCT+TGG - chr4_1:81883792-81883811 Msa0577550:exon 40.0%
! AGAGCAACTTCTAGGAAATG+TGG - chr4_1:81883976-81883995 Msa0577550:intron 40.0%
CAGCATGTCCAACCAAATTT+CGG - chr4_1:81884226-81884245 Msa0577550:intron 40.0%
TCAGACAAATCTTGACCGTT+AGG + chr4_1:81884277-81884296 None:intergenic 40.0%
CGGTCAAGATTTGTCTGAAT+CGG - chr4_1:81884279-81884298 Msa0577550:intron 40.0%
CAAGATTTGTCTGAATCGGT+TGG - chr4_1:81884283-81884302 Msa0577550:intron 40.0%
TTCCTTGGCATATAATGGTG+AGG + chr4_1:81884406-81884425 None:intergenic 40.0%
TCACCATTATATGCCAAGGA+AGG - chr4_1:81884405-81884424 Msa0577550:CDS 40.0%
!!! TTTTTGGAATCAGCGGTGAA+TGG + chr4_1:81884452-81884471 None:intergenic 40.0%
!!! CATGCTCTTTTTGGAATCAG+CGG + chr4_1:81884459-81884478 None:intergenic 40.0%
! GAGAGTGAACATGCTCTTTT+TGG + chr4_1:81884468-81884487 None:intergenic 40.0%
! AAAGAGCATGTTCACTCTCA+AGG - chr4_1:81884469-81884488 Msa0577550:CDS 40.0%
TATGTTGTTGGAGACAGTGA+AGG + chr4_1:81884522-81884541 None:intergenic 40.0%
GTCTCCAACAACATAGTTTC+TGG - chr4_1:81884527-81884546 Msa0577550:intron 40.0%
! CCCAGTTTTCCAAACAACAT+TGG + chr4_1:81884564-81884583 None:intergenic 40.0%
GGAGAACACCAAGAAAATGT+TGG - chr4_1:81884694-81884713 Msa0577550:intron 40.0%
ATGTATATGGTTCCTGCTGT+GGG + chr4_1:81884730-81884749 None:intergenic 40.0%
TATGTATATGGTTCCTGCTG+TGG + chr4_1:81884731-81884750 None:intergenic 40.0%
TGCGATCATCATCTACAAAC+TGG + chr4_1:81885027-81885046 None:intergenic 40.0%
TGATGATCGCAAATGCTACT+TGG - chr4_1:81885036-81885055 Msa0577550:intron 40.0%
GCTACTTGGATGCTAGTTAT+AGG - chr4_1:81885050-81885069 Msa0577550:intron 40.0%
AATCTTGGATGCATCTTAGG+TGG + chr4_1:81885287-81885306 None:intergenic 40.0%
ACTGAGGGGTGAAAAGAAAT+GGG - chr4_1:81885334-81885353 Msa0577550:five_prime_UTR 40.0%
TTGACAATGTCTGCTGCTGT+AGG - chr4_1:81883627-81883646 Msa0577550:three_prime_UTR 45.0%
TGTGCCAGATGAAGATGAAG+AGG - chr4_1:81883758-81883777 Msa0577550:three_prime_UTR 45.0%
GGAAGAGGAAGAACCTAAGT+TGG - chr4_1:81883779-81883798 Msa0577550:three_prime_UTR 45.0%
AATGTGGATGTGTCTGCTGT+TGG - chr4_1:81883992-81884011 Msa0577550:intron 45.0%
CGAAATTTGGTTGGACATGC+TGG + chr4_1:81884228-81884247 None:intergenic 45.0%
AGCCTCACCATTATATGCCA+AGG - chr4_1:81884401-81884420 Msa0577550:CDS 45.0%
AGGCCTTCCTTGGCATATAA+TGG + chr4_1:81884411-81884430 None:intergenic 45.0%
GGAATTGGCAAGATTAGGTC+AGG + chr4_1:81884431-81884450 None:intergenic 45.0%
GAATCAGCGGTGAATGGAAT+TGG + chr4_1:81884446-81884465 None:intergenic 45.0%
AGCATGTTCACTCTCAAGGA+AGG - chr4_1:81884473-81884492 Msa0577550:CDS 45.0%
TCAAGGAAGGAAGCCAATAC+AGG - chr4_1:81884486-81884505 Msa0577550:CDS 45.0%
GAGGCCAGAAACTATGTTGT+TGG + chr4_1:81884534-81884553 None:intergenic 45.0%
GTTTGGAAAACTGGGGTTAG+AGG - chr4_1:81884569-81884588 Msa0577550:intron 45.0%
AACCTACTCAGCAAGAACCA+AGG - chr4_1:81884796-81884815 Msa0577550:intron 45.0%
AATCTGTCTCAGTGTGGTGT+AGG + chr4_1:81885097-81885116 None:intergenic 45.0%
! ATCTGTTAGGAGTGTGACTG+AGG - chr4_1:81885318-81885337 Msa0577550:five_prime_UTR 45.0%
! TCTGTTAGGAGTGTGACTGA+GGG - chr4_1:81885319-81885338 Msa0577550:five_prime_UTR 45.0%
GACTGAGGGGTGAAAAGAAA+TGG - chr4_1:81885333-81885352 Msa0577550:five_prime_UTR 45.0%
CTGAGGGGTGAAAAGAAATG+GGG - chr4_1:81885335-81885354 Msa0577550:five_prime_UTR 45.0%
GAAAAGAAATGGGGTGGGAA+TGG - chr4_1:81885344-81885363 Msa0577550:five_prime_UTR 45.0%
TTGACAATGTCTGCTGCTGT+AGG - chr4_1:81883627-81883646 Msa0577550:three_prime_UTR 45.0%
TGTGCCAGATGAAGATGAAG+AGG - chr4_1:81883758-81883777 Msa0577550:three_prime_UTR 45.0%
GGAAGAGGAAGAACCTAAGT+TGG - chr4_1:81883779-81883798 Msa0577550:three_prime_UTR 45.0%
AATGTGGATGTGTCTGCTGT+TGG - chr4_1:81883992-81884011 Msa0577550:intron 45.0%
CGAAATTTGGTTGGACATGC+TGG + chr4_1:81884228-81884247 None:intergenic 45.0%
AGCCTCACCATTATATGCCA+AGG - chr4_1:81884401-81884420 Msa0577550:CDS 45.0%
AGGCCTTCCTTGGCATATAA+TGG + chr4_1:81884411-81884430 None:intergenic 45.0%
GGAATTGGCAAGATTAGGTC+AGG + chr4_1:81884431-81884450 None:intergenic 45.0%
GAATCAGCGGTGAATGGAAT+TGG + chr4_1:81884446-81884465 None:intergenic 45.0%
AGCATGTTCACTCTCAAGGA+AGG - chr4_1:81884473-81884492 Msa0577550:CDS 45.0%
TCAAGGAAGGAAGCCAATAC+AGG - chr4_1:81884486-81884505 Msa0577550:CDS 45.0%
GAGGCCAGAAACTATGTTGT+TGG + chr4_1:81884534-81884553 None:intergenic 45.0%
GTTTGGAAAACTGGGGTTAG+AGG - chr4_1:81884569-81884588 Msa0577550:intron 45.0%
AACCTACTCAGCAAGAACCA+AGG - chr4_1:81884796-81884815 Msa0577550:intron 45.0%
AATCTGTCTCAGTGTGGTGT+AGG + chr4_1:81885097-81885116 None:intergenic 45.0%
! ATCTGTTAGGAGTGTGACTG+AGG - chr4_1:81885318-81885337 Msa0577550:five_prime_UTR 45.0%
! TCTGTTAGGAGTGTGACTGA+GGG - chr4_1:81885319-81885338 Msa0577550:five_prime_UTR 45.0%
GACTGAGGGGTGAAAAGAAA+TGG - chr4_1:81885333-81885352 Msa0577550:five_prime_UTR 45.0%
CTGAGGGGTGAAAAGAAATG+GGG - chr4_1:81885335-81885354 Msa0577550:five_prime_UTR 45.0%
GAAAAGAAATGGGGTGGGAA+TGG - chr4_1:81885344-81885363 Msa0577550:five_prime_UTR 45.0%
GACATGCTGGACTGCTATAG+AGG + chr4_1:81884215-81884234 None:intergenic 50.0%
CTGATCGTGTCTTGGCCTAA+CGG - chr4_1:81884259-81884278 Msa0577550:intron 50.0%
!! AATCGGTTGGCCTGCTATAG+AGG - chr4_1:81884296-81884315 Msa0577550:CDS 50.0%
TTGGCCTGCTATAGAGGATC+CGG - chr4_1:81884302-81884321 Msa0577550:CDS 50.0%
AGGCTCGCTATCTTCACTTC+TGG + chr4_1:81884386-81884405 None:intergenic 50.0%
AGATTAGGTCAGGCCTTCCT+TGG + chr4_1:81884421-81884440 None:intergenic 50.0%
CCTCTAGCAAACAAGCCTGA+AGG + chr4_1:81884776-81884795 None:intergenic 50.0%
! CCTTCAGGCTTGTTTGCTAG+AGG - chr4_1:81884773-81884792 Msa0577550:intron 50.0%
! TGCCTTGGTTCTTGCTGAGT+AGG + chr4_1:81884801-81884820 None:intergenic 50.0%
! CTGTTAGGAGTGTGACTGAG+GGG - chr4_1:81885320-81885339 Msa0577550:five_prime_UTR 50.0%
AGGGGTGAAAAGAAATGGGG+TGG - chr4_1:81885338-81885357 Msa0577550:five_prime_UTR 50.0%
GGGGTGAAAAGAAATGGGGT+GGG - chr4_1:81885339-81885358 Msa0577550:five_prime_UTR 50.0%
GACATGCTGGACTGCTATAG+AGG + chr4_1:81884215-81884234 None:intergenic 50.0%
CTGATCGTGTCTTGGCCTAA+CGG - chr4_1:81884259-81884278 Msa0577550:intron 50.0%
!! AATCGGTTGGCCTGCTATAG+AGG - chr4_1:81884296-81884315 Msa0577550:CDS 50.0%
TTGGCCTGCTATAGAGGATC+CGG - chr4_1:81884302-81884321 Msa0577550:CDS 50.0%
AGGCTCGCTATCTTCACTTC+TGG + chr4_1:81884386-81884405 None:intergenic 50.0%
AGATTAGGTCAGGCCTTCCT+TGG + chr4_1:81884421-81884440 None:intergenic 50.0%
CCTCTAGCAAACAAGCCTGA+AGG + chr4_1:81884776-81884795 None:intergenic 50.0%
! CCTTCAGGCTTGTTTGCTAG+AGG - chr4_1:81884773-81884792 Msa0577550:intron 50.0%
! TGCCTTGGTTCTTGCTGAGT+AGG + chr4_1:81884801-81884820 None:intergenic 50.0%
! CTGTTAGGAGTGTGACTGAG+GGG - chr4_1:81885320-81885339 Msa0577550:five_prime_UTR 50.0%
AGGGGTGAAAAGAAATGGGG+TGG - chr4_1:81885338-81885357 Msa0577550:five_prime_UTR 50.0%
GGGGTGAAAAGAAATGGGGT+GGG - chr4_1:81885339-81885358 Msa0577550:five_prime_UTR 50.0%
GTGGATGTGTCTGCTGTTGG+AGG - chr4_1:81883995-81884014 Msa0577550:intron 55.0%
GCTGGACTGCTATAGAGGTG+AGG + chr4_1:81884210-81884229 None:intergenic 55.0%
CGTTAGGCCAAGACACGATC+AGG + chr4_1:81884261-81884280 None:intergenic 55.0%
TGGCCTGCTATAGAGGATCC+GGG - chr4_1:81884303-81884322 Msa0577550:CDS 55.0%
GAACCCGGATCCTCTATAGC+AGG + chr4_1:81884309-81884328 None:intergenic 55.0%
GGGAACTTATAGCCCACAGC+AGG - chr4_1:81884715-81884734 Msa0577550:intron 55.0%
GTGGATGTGTCTGCTGTTGG+AGG - chr4_1:81883995-81884014 Msa0577550:intron 55.0%
GCTGGACTGCTATAGAGGTG+AGG + chr4_1:81884210-81884229 None:intergenic 55.0%
CGTTAGGCCAAGACACGATC+AGG + chr4_1:81884261-81884280 None:intergenic 55.0%
TGGCCTGCTATAGAGGATCC+GGG - chr4_1:81884303-81884322 Msa0577550:CDS 55.0%
GAACCCGGATCCTCTATAGC+AGG + chr4_1:81884309-81884328 None:intergenic 55.0%
GGGAACTTATAGCCCACAGC+AGG - chr4_1:81884715-81884734 Msa0577550:intron 55.0%
GGGTGGGAATGGACCCCATG+TGG - chr4_1:81885355-81885374 Msa0577550:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81883538 81885377 81883538 ID=Msa0577550;Name=Msa0577550
chr4_1 mRNA 81883538 81885377 81883538 ID=Msa0577550-mRNA-1;Parent=Msa0577550;Name=Msa0577550-mRNA-1;_AED=0.11;_eAED=0.11;_QI=95|0.66|0.75|1|0.66|0.5|4|269|205
chr4_1 exon 81883538 81883887 81883538 ID=Msa0577550-mRNA-1:exon:29540;Parent=Msa0577550-mRNA-1
chr4_1 exon 81884296 81884543 81884296 ID=Msa0577550-mRNA-1:exon:29539;Parent=Msa0577550-mRNA-1
chr4_1 exon 81884899 81884965 81884899 ID=Msa0577550-mRNA-1:exon:29538;Parent=Msa0577550-mRNA-1
chr4_1 exon 81885061 81885377 81885061 ID=Msa0577550-mRNA-1:exon:29537;Parent=Msa0577550-mRNA-1
chr4_1 five_prime_UTR 81885283 81885377 81885283 ID=Msa0577550-mRNA-1:five_prime_utr;Parent=Msa0577550-mRNA-1
chr4_1 CDS 81885061 81885282 81885061 ID=Msa0577550-mRNA-1:cds;Parent=Msa0577550-mRNA-1
chr4_1 CDS 81884899 81884965 81884899 ID=Msa0577550-mRNA-1:cds;Parent=Msa0577550-mRNA-1
chr4_1 CDS 81884296 81884543 81884296 ID=Msa0577550-mRNA-1:cds;Parent=Msa0577550-mRNA-1
chr4_1 CDS 81883807 81883887 81883807 ID=Msa0577550-mRNA-1:cds;Parent=Msa0577550-mRNA-1
chr4_1 three_prime_UTR 81883538 81883806 81883538 ID=Msa0577550-mRNA-1:three_prime_utr;Parent=Msa0577550-mRNA-1
Gene Sequence

>Msa0577550

ATGTCTGCTGCTGTAGGAGCTGTGTCTACAACCAAAGATGTTTCCTTCAATGCTCATTTGGAGGAAGAGCACAAAAACAAAGGAACCAACAAGGTTGGTGTCAACTATGATGATGCTGAGGAAATTGTGCCAGATGAAGATGAAGAGGAAGAGGAAGAACCTAAGTTGGAATTGGATCTTGGCCCTCAGTTTTCCCTTAAAGAACAGCTTGAGAAAGATAAAGATGATGAAAGCTTGAGGAAATGGAAAGAGCAACTTCTAGGAAATGTGGATGTGTCTGCTGTTGGAGAGAAAATAGAGCCAGAAGTGAAGATAGCGAGCCTCACCATTATATGCCAAGGAAGGCCTGACCTAATCTTGCCAATTCCATTCACCGCTGATTCCAAAAAGAGCATGTTCACTCTCAAGGAAGGAAGCCAATACAGGCTAAAATTCTCCTTCACTGTCTCCAACAACATAGTTTCTGGCCTCAAATATACCAATGTTGTTTGGAAAACTGGGGTTAGAGGTGAGACAGAGACCTCAATTTATGTTTCATTTGTAGATGATGATCGCAAATGCTACTTGGATGCTAGTTATAGGTTTGAGATTCAGAAGAATTGGCCTACACCACACTGA
Protein sequence

>Msa0577550

MSAAVGAVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDDAEEIVPDEDEEEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGNVDVSAVGEKIEPEVKIASLTIICQGRPDLILPIPFTADSKKSMFTLKEGSQYRLKFSFTVSNNIVSGLKYTNVVWKTGVRGETETSIYVSFVDDDRKCYLDASYRFEIQKNWPTPH*