AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1306880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1306880 MtrunA17_Chr8g0377621 80.169 237 13 3 1 205 1 235 1.62e-116 331
Msa1306880 MtrunA17_Chr3g0122221 55.785 242 58 6 1 205 1 230 1.01e-74 224
Msa1306880 MtrunA17_Chr2g0289841 53.672 177 50 2 57 201 56 232 3.67e-58 182
Msa1306880 MtrunA17_Chr3g0120921 50.538 186 60 1 48 201 40 225 1.02e-56 179
Msa1306880 MtrunA17_Chr4g0075711 53.757 173 48 2 61 201 54 226 1.13e-55 176
Msa1306880 MtrunA17_Chr7g0261611 37.696 191 82 5 43 200 32 218 1.43e-35 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1306880 AT3G07880.1 50.267 187 61 1 49 203 53 239 4.41e-59 186
Msa1306880 AT1G12070.1 49.143 175 55 3 61 201 48 222 7.03e-47 154
Msa1306880 AT1G62450.1 45.143 175 62 2 61 201 48 222 5.65e-44 146

Find 80 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCAAGCTGTTCTTTA+AGG 0.103059 8_4:+1819670 None:intergenic
ACCAATGTTGTTTGGAAAAC+TGG 0.188780 8_4:-1818937 Msa1306880:CDS
TTTCATCCCCTTCTATTATA+TGG 0.215093 8_4:-1818303 Msa1306880:three_prime_UTR
TTAAATCTTGGATGCATCTT+AGG 0.217285 8_4:+1818210 None:intergenic
GCTACTTGGATGCTAGTTAT+AGG 0.254947 8_4:-1818447 Msa1306880:CDS
ATGAATTAAATTTCACATAA+AGG 0.300428 8_4:-1818262 Msa1306880:three_prime_UTR
GCAGACATTGTCAAATTTAG+AGG 0.309517 8_4:+1819879 None:intergenic
TTTAATTGTTATTATCTGTT+AGG 0.310142 8_4:-1818192 Msa1306880:three_prime_UTR
AGCATTGAAGGAAACATCTT+TGG 0.319587 8_4:+1819833 None:intergenic
GTCTCCAACAACATAGTTTC+TGG 0.320251 8_4:-1818970 Msa1306880:CDS
AAAAGGAACCATATAATAGA+AGG 0.328086 8_4:+1818295 None:intergenic
TTTCTCAAGCTGTTCTTTAA+GGG 0.328321 8_4:+1819671 None:intergenic
TCTTCCTCTTCATCTTCATC+TGG 0.347069 8_4:+1819735 None:intergenic
ATGATGAAAGCTTGAGGAAA+TGG 0.349670 8_4:-1819546 Msa1306880:CDS
AAATGGAAAGAGCAACTTCT+AGG 0.351067 8_4:-1819529 Msa1306880:CDS
TCAAATATACCAATGTTGTT+TGG 0.363873 8_4:-1818945 Msa1306880:CDS
GAATCAGCGGTGAATGGAAT+TGG 0.371922 8_4:+1819054 None:intergenic
GGTTTGAGATTCAGAAGAAT+TGG 0.376482 8_4:-1818426 Msa1306880:CDS
AGGCCTTCCTTGGCATATAA+TGG 0.379225 8_4:+1819089 None:intergenic
TGTTTCCTTCAATGCTCATT+TGG 0.380600 8_4:-1819826 Msa1306880:CDS
GGAAGAACCTAAGTTGGAAT+TGG 0.382333 8_4:-1819712 Msa1306880:CDS
TTCCTCCAAATGAGCATTGA+AGG 0.385347 8_4:+1819821 None:intergenic
GACTGAGGGGTGAAAAGAAA+TGG 0.401381 8_4:-1818164 Msa1306880:three_prime_UTR
AGGCTCGCTATCTTCACTTC+TGG 0.402038 8_4:+1819114 None:intergenic
CCTAAGTTGGAATTGGATCT+TGG 0.402975 8_4:-1819705 Msa1306880:CDS
CCAAGATCCAATTCCAACTT+AGG 0.415301 8_4:+1819705 None:intergenic
TGTTTATTAAACTAAGTTAG+TGG 0.416620 8_4:-1818362 Msa1306880:three_prime_UTR
ACTGAGGGGTGAAAAGAAAT+GGG 0.417413 8_4:-1818163 Msa1306880:three_prime_UTR
ATTACATTACCAAGGGATAT+AGG 0.417448 8_4:-1819914 Msa1306880:five_prime_UTR
CCTTGGTAATGTAATATGTA+TGG 0.425085 8_4:+1819922 None:intergenic
CCAATGTTGTTTGGAAAACT+GGG 0.429548 8_4:-1818936 Msa1306880:CDS
AGATAATAACAATTAAATCT+TGG 0.436258 8_4:+1818198 None:intergenic
GTTTGGAAAACTGGGGTTAG+AGG 0.436517 8_4:-1818928 Msa1306880:CDS
GAGGCCAGAAACTATGTTGT+TGG 0.443718 8_4:+1818966 None:intergenic
GGAAGAGGAAGAACCTAAGT+TGG 0.456835 8_4:-1819718 Msa1306880:CDS
ATAGTTGACACCAACCTTGT+TGG 0.461291 8_4:+1819779 None:intergenic
GAAAAGAAATGGGGTGGGAA+TGG 0.466205 8_4:-1818153 Msa1306880:three_prime_UTR
AGATTAGGTCAGGCCTTCCT+TGG 0.466881 8_4:+1819079 None:intergenic
AAACAACATTGGTATATTTG+AGG 0.467217 8_4:+1818947 None:intergenic
TCAAGGAAGGAAGCCAATAC+AGG 0.471561 8_4:-1819011 Msa1306880:CDS
TTCCTTCAATGCTCATTTGG+AGG 0.498763 8_4:-1819823 Msa1306880:CDS
TGATGATCGCAAATGCTACT+TGG 0.504667 8_4:-1818461 Msa1306880:CDS
AGATGAAGATGAAGAGGAAG+AGG 0.507523 8_4:-1819733 Msa1306880:CDS
GGAATTGGCAAGATTAGGTC+AGG 0.511505 8_4:+1819069 None:intergenic
AATCTGTCTCAGTGTGGTGT+AGG 0.514298 8_4:+1818403 None:intergenic
AAAGAGCATGTTCACTCTCA+AGG 0.517312 8_4:-1819028 Msa1306880:CDS
AAAGGAACCATATAATAGAA+GGG 0.530739 8_4:+1818296 None:intergenic
TGAATGGAATTGGCAAGATT+AGG 0.534268 8_4:+1819064 None:intergenic
AATCTTGGATGCATCTTAGG+TGG 0.541371 8_4:+1818213 None:intergenic
TGTGCCAGATGAAGATGAAG+AGG 0.547332 8_4:-1819739 Msa1306880:CDS
GTGGATGTGTCTGCTGTTGG+AGG 0.550509 8_4:-1819502 Msa1306880:intron
TCTAACTGAAACATAAATTG+AGG 0.554988 8_4:+1818894 None:intergenic
AGGGGTGAAAAGAAATGGGG+TGG 0.559682 8_4:-1818159 Msa1306880:three_prime_UTR
TATTTCTGTGCACATCCACA+TGG 0.562058 8_4:+1818127 None:intergenic
AATGTGGATGTGTCTGCTGT+TGG 0.567791 8_4:-1819505 Msa1306880:CDS
AGAGCAACTTCTAGGAAATG+TGG 0.570308 8_4:-1819521 Msa1306880:CDS
TTCCTTGGCATATAATGGTG+AGG 0.572917 8_4:+1819094 None:intergenic
TAAAGGATGATGAAAGCTTG+AGG 0.574330 8_4:-1819552 Msa1306880:intron
AGCCTCACCATTATATGCCA+AGG 0.579121 8_4:-1819096 Msa1306880:CDS
ATCTGTTAGGAGTGTGACTG+AGG 0.600412 8_4:-1818179 Msa1306880:three_prime_UTR
AGCATGTTCACTCTCAAGGA+AGG 0.602637 8_4:-1819024 Msa1306880:CDS
AACAAAGGAACCAACAAGGT+TGG 0.603340 8_4:-1819789 Msa1306880:CDS
GTTCTTTAAGGGAAAACTGA+GGG 0.605683 8_4:+1819682 None:intergenic
GGGTGGGAATGGACCCCATG+TGG 0.607426 8_4:-1818142 Msa1306880:three_prime_UTR
ATTTCTGTGCACATCCACAT+GGG 0.613728 8_4:+1818128 None:intergenic
TTGACAATGTCTGCTGCTGT+AGG 0.616083 8_4:-1819870 Msa1306880:exon
TCACCATTATATGCCAAGGA+AGG 0.618936 8_4:-1819092 Msa1306880:CDS
AAGGAACCATATAATAGAAG+GGG 0.620485 8_4:+1818297 None:intergenic
CATACATATTACATTACCAA+GGG 0.621345 8_4:-1819921 Msa1306880:five_prime_UTR
TATGTTGTTGGAGACAGTGA+AGG 0.635616 8_4:+1818978 None:intergenic
CCATACATATTACATTACCA+AGG 0.643408 8_4:-1819922 Msa1306880:five_prime_UTR
TCTGTTAGGAGTGTGACTGA+GGG 0.643817 8_4:-1818178 Msa1306880:three_prime_UTR
GGGGTGAAAAGAAATGGGGT+GGG 0.646062 8_4:-1818158 Msa1306880:three_prime_UTR
CAATGTTGTTTGGAAAACTG+GGG 0.646093 8_4:-1818935 Msa1306880:CDS
CTGAGGGGTGAAAAGAAATG+GGG 0.656530 8_4:-1818162 Msa1306880:three_prime_UTR
TGTTCTTTAAGGGAAAACTG+AGG 0.676471 8_4:+1819681 None:intergenic
TTTCTGTGCACATCCACATG+GGG 0.681268 8_4:+1818129 None:intergenic
AATTTCAATCTGTCTCAGTG+TGG 0.687125 8_4:+1818397 None:intergenic
CTGTTAGGAGTGTGACTGAG+GGG 0.699002 8_4:-1818177 Msa1306880:three_prime_UTR
TGTCAACTATGATGATGCTG+AGG 0.722211 8_4:-1819766 Msa1306880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTTTTTATTTTATT+TGG - chr8_4:1819577-1819596 Msa1306880:intron 0.0%
!!! TTAATTTTTTTTATTTTATT+TGG - chr8_4:1819577-1819596 Msa1306880:intron 0.0%
!!! AATTTTGTTTTGTATTTTAA+AGG - chr8_4:1818532-1818551 Msa1306880:intron 10.0%
!!! AATTTTGTTTTGTATTTTAA+AGG - chr8_4:1818532-1818551 Msa1306880:intron 10.0%
!! ATAAAAGAAGTCAATTAAAT+TGG + chr8_4:1818734-1818753 None:intergenic 15.0%
!! TTATGAATTTATTCAAATAG+TGG - chr8_4:1819277-1819296 Msa1306880:intron 15.0%
!! AATTCATTACAATACAAAAA+AGG + chr8_4:1819826-1819845 None:intergenic 15.0%
!! ATGAATTAAATTTCACATAA+AGG - chr8_4:1819839-1819858 Msa1306880:CDS 15.0%
!! AGATAATAACAATTAAATCT+TGG + chr8_4:1819906-1819925 None:intergenic 15.0%
!!! TTTAATTGTTATTATCTGTT+AGG - chr8_4:1819909-1819928 Msa1306880:five_prime_UTR 15.0%
!! ATAAAAGAAGTCAATTAAAT+TGG + chr8_4:1818734-1818753 None:intergenic 15.0%
!! TTATGAATTTATTCAAATAG+TGG - chr8_4:1819277-1819296 Msa1306880:intron 15.0%
!! AATTCATTACAATACAAAAA+AGG + chr8_4:1819826-1819845 None:intergenic 15.0%
!! ATGAATTAAATTTCACATAA+AGG - chr8_4:1819839-1819858 Msa1306880:CDS 15.0%
!! AGATAATAACAATTAAATCT+TGG + chr8_4:1819906-1819925 None:intergenic 15.0%
!!! TTTAATTGTTATTATCTGTT+AGG - chr8_4:1819909-1819928 Msa1306880:five_prime_UTR 15.0%
!!! TTTAATTGACTTCTTTTATC+AGG - chr8_4:1818735-1818754 Msa1306880:intron 20.0%
!! TGTTTATTAAACTAAGTTAG+TGG - chr8_4:1819739-1819758 Msa1306880:CDS 20.0%
!!! TTTAATTGACTTCTTTTATC+AGG - chr8_4:1818735-1818754 Msa1306880:intron 20.0%
!! TGTTTATTAAACTAAGTTAG+TGG - chr8_4:1819739-1819758 Msa1306880:CDS 20.0%
! CATACATATTACATTACCAA+GGG - chr8_4:1818180-1818199 Msa1306880:three_prime_UTR 25.0%
!!! AGTAAGGTTCTTTTTTGAAA+TGG - chr8_4:1818458-1818477 Msa1306880:CDS 25.0%
!!! GTAGTGTTTTTTTGATGAAT+TGG - chr8_4:1818508-1818527 Msa1306880:intron 25.0%
! AGAAGAAAAACAAAATCGAA+AGG + chr8_4:1818635-1818654 None:intergenic 25.0%
!!! CTTCTGAAACAATTCTTAAA+TGG - chr8_4:1818650-1818669 Msa1306880:intron 25.0%
! TCAATTAAATTGGAAGAAGA+GGG + chr8_4:1818724-1818743 None:intergenic 25.0%
! TGTTAATAAAAACAAGAACC+CGG + chr8_4:1818928-1818947 None:intergenic 25.0%
! AAACAACATTGGTATATTTG+AGG + chr8_4:1819157-1819176 None:intergenic 25.0%
! TCAAATATACCAATGTTGTT+TGG - chr8_4:1819156-1819175 Msa1306880:intron 25.0%
! TCTAACTGAAACATAAATTG+AGG + chr8_4:1819210-1819229 None:intergenic 25.0%
! TTTGAATTGTCTTAGTTATG+TGG - chr8_4:1819251-1819270 Msa1306880:intron 25.0%
! TCTTCCAATTCATATGTATA+TGG + chr8_4:1819347-1819366 None:intergenic 25.0%
! TGAAACTAACACATACTTAT+CGG - chr8_4:1819604-1819623 Msa1306880:intron 25.0%
! AAAGGAACCATATAATAGAA+GGG + chr8_4:1819808-1819827 None:intergenic 25.0%
! AAAAGGAACCATATAATAGA+AGG + chr8_4:1819809-1819828 None:intergenic 25.0%
! CATACATATTACATTACCAA+GGG - chr8_4:1818180-1818199 Msa1306880:three_prime_UTR 25.0%
!!! AGTAAGGTTCTTTTTTGAAA+TGG - chr8_4:1818458-1818477 Msa1306880:CDS 25.0%
!!! GTAGTGTTTTTTTGATGAAT+TGG - chr8_4:1818508-1818527 Msa1306880:intron 25.0%
! AGAAGAAAAACAAAATCGAA+AGG + chr8_4:1818635-1818654 None:intergenic 25.0%
!!! CTTCTGAAACAATTCTTAAA+TGG - chr8_4:1818650-1818669 Msa1306880:intron 25.0%
! TCAATTAAATTGGAAGAAGA+GGG + chr8_4:1818724-1818743 None:intergenic 25.0%
! TGTTAATAAAAACAAGAACC+CGG + chr8_4:1818928-1818947 None:intergenic 25.0%
! AAACAACATTGGTATATTTG+AGG + chr8_4:1819157-1819176 None:intergenic 25.0%
! TCAAATATACCAATGTTGTT+TGG - chr8_4:1819156-1819175 Msa1306880:intron 25.0%
! TCTAACTGAAACATAAATTG+AGG + chr8_4:1819210-1819229 None:intergenic 25.0%
! TTTGAATTGTCTTAGTTATG+TGG - chr8_4:1819251-1819270 Msa1306880:intron 25.0%
! TCTTCCAATTCATATGTATA+TGG + chr8_4:1819347-1819366 None:intergenic 25.0%
! TGAAACTAACACATACTTAT+CGG - chr8_4:1819604-1819623 Msa1306880:intron 25.0%
! AAAGGAACCATATAATAGAA+GGG + chr8_4:1819808-1819827 None:intergenic 25.0%
! AAAAGGAACCATATAATAGA+AGG + chr8_4:1819809-1819828 None:intergenic 25.0%
!! CCTTGGTAATGTAATATGTA+TGG + chr8_4:1818182-1818201 None:intergenic 30.0%
CCATACATATTACATTACCA+AGG - chr8_4:1818179-1818198 Msa1306880:three_prime_UTR 30.0%
ATTACATTACCAAGGGATAT+AGG - chr8_4:1818187-1818206 Msa1306880:three_prime_UTR 30.0%
TTTCTCAAGCTGTTCTTTAA+GGG + chr8_4:1818433-1818452 None:intergenic 30.0%
AGCTTGAGAAAGATAAAGTA+AGG - chr8_4:1818442-1818461 Msa1306880:CDS 30.0%
GTCAATTAAATTGGAAGAAG+AGG + chr8_4:1818725-1818744 None:intergenic 30.0%
! CTCTCATCTTTTACATTTAC+AGG - chr8_4:1818774-1818793 Msa1306880:intron 30.0%
! ATAAGTTCCCAACATTTTCT+TGG + chr8_4:1819309-1819328 None:intergenic 30.0%
GGAACCATATACATATGAAT+TGG - chr8_4:1819340-1819359 Msa1306880:intron 30.0%
! ATTTTGTAAGTTTCTTGCCT+TGG + chr8_4:1819420-1819439 None:intergenic 30.0%
TTTCATCCCCTTCTATTATA+TGG - chr8_4:1819798-1819817 Msa1306880:CDS 30.0%
AAGGAACCATATAATAGAAG+GGG + chr8_4:1819807-1819826 None:intergenic 30.0%
TTAAATCTTGGATGCATCTT+AGG + chr8_4:1819894-1819913 None:intergenic 30.0%
!! CCTTGGTAATGTAATATGTA+TGG + chr8_4:1818182-1818201 None:intergenic 30.0%
CCATACATATTACATTACCA+AGG - chr8_4:1818179-1818198 Msa1306880:three_prime_UTR 30.0%
ATTACATTACCAAGGGATAT+AGG - chr8_4:1818187-1818206 Msa1306880:three_prime_UTR 30.0%
TTTCTCAAGCTGTTCTTTAA+GGG + chr8_4:1818433-1818452 None:intergenic 30.0%
AGCTTGAGAAAGATAAAGTA+AGG - chr8_4:1818442-1818461 Msa1306880:CDS 30.0%
GTCAATTAAATTGGAAGAAG+AGG + chr8_4:1818725-1818744 None:intergenic 30.0%
! CTCTCATCTTTTACATTTAC+AGG - chr8_4:1818774-1818793 Msa1306880:intron 30.0%
! ATAAGTTCCCAACATTTTCT+TGG + chr8_4:1819309-1819328 None:intergenic 30.0%
GGAACCATATACATATGAAT+TGG - chr8_4:1819340-1819359 Msa1306880:intron 30.0%
! ATTTTGTAAGTTTCTTGCCT+TGG + chr8_4:1819420-1819439 None:intergenic 30.0%
TTTCATCCCCTTCTATTATA+TGG - chr8_4:1819798-1819817 Msa1306880:CDS 30.0%
AAGGAACCATATAATAGAAG+GGG + chr8_4:1819807-1819826 None:intergenic 30.0%
TTAAATCTTGGATGCATCTT+AGG + chr8_4:1819894-1819913 None:intergenic 30.0%
GCAGACATTGTCAAATTTAG+AGG + chr8_4:1818225-1818244 None:intergenic 35.0%
! AGCATTGAAGGAAACATCTT+TGG + chr8_4:1818271-1818290 None:intergenic 35.0%
TGTTTCCTTCAATGCTCATT+TGG - chr8_4:1818275-1818294 Msa1306880:three_prime_UTR 35.0%
CAAAAACAAAGGAACCAACA+AGG - chr8_4:1818308-1818327 Msa1306880:three_prime_UTR 35.0%
! GTTCTTTAAGGGAAAACTGA+GGG + chr8_4:1818422-1818441 None:intergenic 35.0%
! TGTTCTTTAAGGGAAAACTG+AGG + chr8_4:1818423-1818442 None:intergenic 35.0%
CTTTCTCAAGCTGTTCTTTA+AGG + chr8_4:1818434-1818453 None:intergenic 35.0%
TAAAGGATGATGAAAGCTTG+AGG - chr8_4:1818549-1818568 Msa1306880:intron 35.0%
ATGATGAAAGCTTGAGGAAA+TGG - chr8_4:1818555-1818574 Msa1306880:intron 35.0%
AAATGGAAAGAGCAACTTCT+AGG - chr8_4:1818572-1818591 Msa1306880:intron 35.0%
!!! TTTTTTGGCCGAAATTTGGT+TGG + chr8_4:1818841-1818860 None:intergenic 35.0%
!!! AGGATTTTTTGGCCGAAATT+TGG + chr8_4:1818845-1818864 None:intergenic 35.0%
!!! AAGACACGATCAGGATTTTT+TGG + chr8_4:1818856-1818875 None:intergenic 35.0%
AAAAAATCCTGATCGTGTCT+TGG - chr8_4:1818855-1818874 Msa1306880:intron 35.0%
TCTCTACATCACAACAAGAT+AGG + chr8_4:1818959-1818978 None:intergenic 35.0%
TGAATGGAATTGGCAAGATT+AGG + chr8_4:1819040-1819059 None:intergenic 35.0%
!! AGGAGAATTTTAGCCTGTAT+TGG + chr8_4:1819106-1819125 None:intergenic 35.0%
! ACCAATGTTGTTTGGAAAAC+TGG - chr8_4:1819164-1819183 Msa1306880:intron 35.0%
! CCAATGTTGTTTGGAAAACT+GGG - chr8_4:1819165-1819184 Msa1306880:intron 35.0%
! CAATGTTGTTTGGAAAACTG+GGG - chr8_4:1819166-1819185 Msa1306880:intron 35.0%
GAGAACACCAAGAAAATGTT+GGG - chr8_4:1819299-1819318 Msa1306880:intron 35.0%
GAAGAAGAAATTACTCCTTC+AGG - chr8_4:1819362-1819381 Msa1306880:intron 35.0%
TGTCAATGCTACACAAGTAA+CGG + chr8_4:1819474-1819493 None:intergenic 35.0%
! GGTTTGAGATTCAGAAGAAT+TGG - chr8_4:1819675-1819694 Msa1306880:CDS 35.0%
AATTTCAATCTGTCTCAGTG+TGG + chr8_4:1819707-1819726 None:intergenic 35.0%
GCAGACATTGTCAAATTTAG+AGG + chr8_4:1818225-1818244 None:intergenic 35.0%
! AGCATTGAAGGAAACATCTT+TGG + chr8_4:1818271-1818290 None:intergenic 35.0%
TGTTTCCTTCAATGCTCATT+TGG - chr8_4:1818275-1818294 Msa1306880:three_prime_UTR 35.0%
CAAAAACAAAGGAACCAACA+AGG - chr8_4:1818308-1818327 Msa1306880:three_prime_UTR 35.0%
! GTTCTTTAAGGGAAAACTGA+GGG + chr8_4:1818422-1818441 None:intergenic 35.0%
! TGTTCTTTAAGGGAAAACTG+AGG + chr8_4:1818423-1818442 None:intergenic 35.0%
CTTTCTCAAGCTGTTCTTTA+AGG + chr8_4:1818434-1818453 None:intergenic 35.0%
TAAAGGATGATGAAAGCTTG+AGG - chr8_4:1818549-1818568 Msa1306880:intron 35.0%
ATGATGAAAGCTTGAGGAAA+TGG - chr8_4:1818555-1818574 Msa1306880:intron 35.0%
AAATGGAAAGAGCAACTTCT+AGG - chr8_4:1818572-1818591 Msa1306880:intron 35.0%
!!! TTTTTTGGCCGAAATTTGGT+TGG + chr8_4:1818841-1818860 None:intergenic 35.0%
!!! AGGATTTTTTGGCCGAAATT+TGG + chr8_4:1818845-1818864 None:intergenic 35.0%
!!! AAGACACGATCAGGATTTTT+TGG + chr8_4:1818856-1818875 None:intergenic 35.0%
AAAAAATCCTGATCGTGTCT+TGG - chr8_4:1818855-1818874 Msa1306880:intron 35.0%
TCTCTACATCACAACAAGAT+AGG + chr8_4:1818959-1818978 None:intergenic 35.0%
TGAATGGAATTGGCAAGATT+AGG + chr8_4:1819040-1819059 None:intergenic 35.0%
!! AGGAGAATTTTAGCCTGTAT+TGG + chr8_4:1819106-1819125 None:intergenic 35.0%
! ACCAATGTTGTTTGGAAAAC+TGG - chr8_4:1819164-1819183 Msa1306880:intron 35.0%
! CCAATGTTGTTTGGAAAACT+GGG - chr8_4:1819165-1819184 Msa1306880:intron 35.0%
! CAATGTTGTTTGGAAAACTG+GGG - chr8_4:1819166-1819185 Msa1306880:intron 35.0%
GAGAACACCAAGAAAATGTT+GGG - chr8_4:1819299-1819318 Msa1306880:intron 35.0%
GAAGAAGAAATTACTCCTTC+AGG - chr8_4:1819362-1819381 Msa1306880:intron 35.0%
TGTCAATGCTACACAAGTAA+CGG + chr8_4:1819474-1819493 None:intergenic 35.0%
! GGTTTGAGATTCAGAAGAAT+TGG - chr8_4:1819675-1819694 Msa1306880:CDS 35.0%
AATTTCAATCTGTCTCAGTG+TGG + chr8_4:1819707-1819726 None:intergenic 35.0%
! GTTTTGCTTCCTATATCCCT+TGG + chr8_4:1818199-1818218 None:intergenic 40.0%
TTCCTTCAATGCTCATTTGG+AGG - chr8_4:1818278-1818297 Msa1306880:three_prime_UTR 40.0%
!! TTCCTCCAAATGAGCATTGA+AGG + chr8_4:1818283-1818302 None:intergenic 40.0%
! GAGGAAGAGCACAAAAACAA+AGG - chr8_4:1818297-1818316 Msa1306880:three_prime_UTR 40.0%
AACAAAGGAACCAACAAGGT+TGG - chr8_4:1818312-1818331 Msa1306880:three_prime_UTR 40.0%
ATAGTTGACACCAACCTTGT+TGG + chr8_4:1818325-1818344 None:intergenic 40.0%
! TGTCAACTATGATGATGCTG+AGG - chr8_4:1818335-1818354 Msa1306880:three_prime_UTR 40.0%
TCTTCCTCTTCATCTTCATC+TGG + chr8_4:1818369-1818388 None:intergenic 40.0%
AGATGAAGATGAAGAGGAAG+AGG - chr8_4:1818368-1818387 Msa1306880:three_prime_UTR 40.0%
! GGAAGAACCTAAGTTGGAAT+TGG - chr8_4:1818389-1818408 Msa1306880:three_prime_UTR 40.0%
CCAAGATCCAATTCCAACTT+AGG + chr8_4:1818399-1818418 None:intergenic 40.0%
! CCTAAGTTGGAATTGGATCT+TGG - chr8_4:1818396-1818415 Msa1306880:exon 40.0%
! AGAGCAACTTCTAGGAAATG+TGG - chr8_4:1818580-1818599 Msa1306880:intron 40.0%
CAGCATGTCCAACCAAATTT+CGG - chr8_4:1818830-1818849 Msa1306880:intron 40.0%
TCAGACAAATCTTGACCGTT+AGG + chr8_4:1818881-1818900 None:intergenic 40.0%
CGGTCAAGATTTGTCTGAAT+CGG - chr8_4:1818883-1818902 Msa1306880:intron 40.0%
CAAGATTTGTCTGAATCGGT+TGG - chr8_4:1818887-1818906 Msa1306880:intron 40.0%
TTCCTTGGCATATAATGGTG+AGG + chr8_4:1819010-1819029 None:intergenic 40.0%
TCACCATTATATGCCAAGGA+AGG - chr8_4:1819009-1819028 Msa1306880:CDS 40.0%
!!! TTTTTGGAATCAGCGGTGAA+TGG + chr8_4:1819056-1819075 None:intergenic 40.0%
!!! CATGCTCTTTTTGGAATCAG+CGG + chr8_4:1819063-1819082 None:intergenic 40.0%
! GAGAGTGAACATGCTCTTTT+TGG + chr8_4:1819072-1819091 None:intergenic 40.0%
! AAAGAGCATGTTCACTCTCA+AGG - chr8_4:1819073-1819092 Msa1306880:CDS 40.0%
TATGTTGTTGGAGACAGTGA+AGG + chr8_4:1819126-1819145 None:intergenic 40.0%
GTCTCCAACAACATAGTTTC+TGG - chr8_4:1819131-1819150 Msa1306880:intron 40.0%
! CCCAGTTTTCCAAACAACAT+TGG + chr8_4:1819168-1819187 None:intergenic 40.0%
GGAGAACACCAAGAAAATGT+TGG - chr8_4:1819298-1819317 Msa1306880:intron 40.0%
ATGTATATGGTTCCTGCTGT+GGG + chr8_4:1819334-1819353 None:intergenic 40.0%
TATGTATATGGTTCCTGCTG+TGG + chr8_4:1819335-1819354 None:intergenic 40.0%
TGCGATCATCATCTACAAAC+TGG + chr8_4:1819631-1819650 None:intergenic 40.0%
TGATGATCGCAAATGCTACT+TGG - chr8_4:1819640-1819659 Msa1306880:intron 40.0%
GCTACTTGGATGCTAGTTAT+AGG - chr8_4:1819654-1819673 Msa1306880:intron 40.0%
AATCTTGGATGCATCTTAGG+TGG + chr8_4:1819891-1819910 None:intergenic 40.0%
ACTGAGGGGTGAAAAGAAAT+GGG - chr8_4:1819938-1819957 Msa1306880:five_prime_UTR 40.0%
! GTTTTGCTTCCTATATCCCT+TGG + chr8_4:1818199-1818218 None:intergenic 40.0%
TTCCTTCAATGCTCATTTGG+AGG - chr8_4:1818278-1818297 Msa1306880:three_prime_UTR 40.0%
!! TTCCTCCAAATGAGCATTGA+AGG + chr8_4:1818283-1818302 None:intergenic 40.0%
! GAGGAAGAGCACAAAAACAA+AGG - chr8_4:1818297-1818316 Msa1306880:three_prime_UTR 40.0%
AACAAAGGAACCAACAAGGT+TGG - chr8_4:1818312-1818331 Msa1306880:three_prime_UTR 40.0%
ATAGTTGACACCAACCTTGT+TGG + chr8_4:1818325-1818344 None:intergenic 40.0%
! TGTCAACTATGATGATGCTG+AGG - chr8_4:1818335-1818354 Msa1306880:three_prime_UTR 40.0%
TCTTCCTCTTCATCTTCATC+TGG + chr8_4:1818369-1818388 None:intergenic 40.0%
AGATGAAGATGAAGAGGAAG+AGG - chr8_4:1818368-1818387 Msa1306880:three_prime_UTR 40.0%
! GGAAGAACCTAAGTTGGAAT+TGG - chr8_4:1818389-1818408 Msa1306880:three_prime_UTR 40.0%
CCAAGATCCAATTCCAACTT+AGG + chr8_4:1818399-1818418 None:intergenic 40.0%
! CCTAAGTTGGAATTGGATCT+TGG - chr8_4:1818396-1818415 Msa1306880:exon 40.0%
! AGAGCAACTTCTAGGAAATG+TGG - chr8_4:1818580-1818599 Msa1306880:intron 40.0%
CAGCATGTCCAACCAAATTT+CGG - chr8_4:1818830-1818849 Msa1306880:intron 40.0%
TCAGACAAATCTTGACCGTT+AGG + chr8_4:1818881-1818900 None:intergenic 40.0%
CGGTCAAGATTTGTCTGAAT+CGG - chr8_4:1818883-1818902 Msa1306880:intron 40.0%
CAAGATTTGTCTGAATCGGT+TGG - chr8_4:1818887-1818906 Msa1306880:intron 40.0%
TTCCTTGGCATATAATGGTG+AGG + chr8_4:1819010-1819029 None:intergenic 40.0%
TCACCATTATATGCCAAGGA+AGG - chr8_4:1819009-1819028 Msa1306880:CDS 40.0%
!!! TTTTTGGAATCAGCGGTGAA+TGG + chr8_4:1819056-1819075 None:intergenic 40.0%
!!! CATGCTCTTTTTGGAATCAG+CGG + chr8_4:1819063-1819082 None:intergenic 40.0%
! GAGAGTGAACATGCTCTTTT+TGG + chr8_4:1819072-1819091 None:intergenic 40.0%
! AAAGAGCATGTTCACTCTCA+AGG - chr8_4:1819073-1819092 Msa1306880:CDS 40.0%
TATGTTGTTGGAGACAGTGA+AGG + chr8_4:1819126-1819145 None:intergenic 40.0%
GTCTCCAACAACATAGTTTC+TGG - chr8_4:1819131-1819150 Msa1306880:intron 40.0%
! CCCAGTTTTCCAAACAACAT+TGG + chr8_4:1819168-1819187 None:intergenic 40.0%
GGAGAACACCAAGAAAATGT+TGG - chr8_4:1819298-1819317 Msa1306880:intron 40.0%
ATGTATATGGTTCCTGCTGT+GGG + chr8_4:1819334-1819353 None:intergenic 40.0%
TATGTATATGGTTCCTGCTG+TGG + chr8_4:1819335-1819354 None:intergenic 40.0%
TGCGATCATCATCTACAAAC+TGG + chr8_4:1819631-1819650 None:intergenic 40.0%
TGATGATCGCAAATGCTACT+TGG - chr8_4:1819640-1819659 Msa1306880:intron 40.0%
GCTACTTGGATGCTAGTTAT+AGG - chr8_4:1819654-1819673 Msa1306880:intron 40.0%
AATCTTGGATGCATCTTAGG+TGG + chr8_4:1819891-1819910 None:intergenic 40.0%
ACTGAGGGGTGAAAAGAAAT+GGG - chr8_4:1819938-1819957 Msa1306880:five_prime_UTR 40.0%
TTGACAATGTCTGCTGCTGT+AGG - chr8_4:1818231-1818250 Msa1306880:three_prime_UTR 45.0%
TGTGCCAGATGAAGATGAAG+AGG - chr8_4:1818362-1818381 Msa1306880:three_prime_UTR 45.0%
GGAAGAGGAAGAACCTAAGT+TGG - chr8_4:1818383-1818402 Msa1306880:three_prime_UTR 45.0%
AATGTGGATGTGTCTGCTGT+TGG - chr8_4:1818596-1818615 Msa1306880:intron 45.0%
CGAAATTTGGTTGGACATGC+TGG + chr8_4:1818832-1818851 None:intergenic 45.0%
AGCCTCACCATTATATGCCA+AGG - chr8_4:1819005-1819024 Msa1306880:CDS 45.0%
AGGCCTTCCTTGGCATATAA+TGG + chr8_4:1819015-1819034 None:intergenic 45.0%
GGAATTGGCAAGATTAGGTC+AGG + chr8_4:1819035-1819054 None:intergenic 45.0%
GAATCAGCGGTGAATGGAAT+TGG + chr8_4:1819050-1819069 None:intergenic 45.0%
AGCATGTTCACTCTCAAGGA+AGG - chr8_4:1819077-1819096 Msa1306880:CDS 45.0%
TCAAGGAAGGAAGCCAATAC+AGG - chr8_4:1819090-1819109 Msa1306880:CDS 45.0%
GAGGCCAGAAACTATGTTGT+TGG + chr8_4:1819138-1819157 None:intergenic 45.0%
GTTTGGAAAACTGGGGTTAG+AGG - chr8_4:1819173-1819192 Msa1306880:intron 45.0%
AACCTACTCAGCAAGAACCA+AGG - chr8_4:1819400-1819419 Msa1306880:intron 45.0%
AATCTGTCTCAGTGTGGTGT+AGG + chr8_4:1819701-1819720 None:intergenic 45.0%
! ATCTGTTAGGAGTGTGACTG+AGG - chr8_4:1819922-1819941 Msa1306880:five_prime_UTR 45.0%
! TCTGTTAGGAGTGTGACTGA+GGG - chr8_4:1819923-1819942 Msa1306880:five_prime_UTR 45.0%
GACTGAGGGGTGAAAAGAAA+TGG - chr8_4:1819937-1819956 Msa1306880:five_prime_UTR 45.0%
CTGAGGGGTGAAAAGAAATG+GGG - chr8_4:1819939-1819958 Msa1306880:five_prime_UTR 45.0%
GAAAAGAAATGGGGTGGGAA+TGG - chr8_4:1819948-1819967 Msa1306880:five_prime_UTR 45.0%
TTGACAATGTCTGCTGCTGT+AGG - chr8_4:1818231-1818250 Msa1306880:three_prime_UTR 45.0%
TGTGCCAGATGAAGATGAAG+AGG - chr8_4:1818362-1818381 Msa1306880:three_prime_UTR 45.0%
GGAAGAGGAAGAACCTAAGT+TGG - chr8_4:1818383-1818402 Msa1306880:three_prime_UTR 45.0%
AATGTGGATGTGTCTGCTGT+TGG - chr8_4:1818596-1818615 Msa1306880:intron 45.0%
CGAAATTTGGTTGGACATGC+TGG + chr8_4:1818832-1818851 None:intergenic 45.0%
AGCCTCACCATTATATGCCA+AGG - chr8_4:1819005-1819024 Msa1306880:CDS 45.0%
AGGCCTTCCTTGGCATATAA+TGG + chr8_4:1819015-1819034 None:intergenic 45.0%
GGAATTGGCAAGATTAGGTC+AGG + chr8_4:1819035-1819054 None:intergenic 45.0%
GAATCAGCGGTGAATGGAAT+TGG + chr8_4:1819050-1819069 None:intergenic 45.0%
AGCATGTTCACTCTCAAGGA+AGG - chr8_4:1819077-1819096 Msa1306880:CDS 45.0%
TCAAGGAAGGAAGCCAATAC+AGG - chr8_4:1819090-1819109 Msa1306880:CDS 45.0%
GAGGCCAGAAACTATGTTGT+TGG + chr8_4:1819138-1819157 None:intergenic 45.0%
GTTTGGAAAACTGGGGTTAG+AGG - chr8_4:1819173-1819192 Msa1306880:intron 45.0%
AACCTACTCAGCAAGAACCA+AGG - chr8_4:1819400-1819419 Msa1306880:intron 45.0%
AATCTGTCTCAGTGTGGTGT+AGG + chr8_4:1819701-1819720 None:intergenic 45.0%
! ATCTGTTAGGAGTGTGACTG+AGG - chr8_4:1819922-1819941 Msa1306880:five_prime_UTR 45.0%
! TCTGTTAGGAGTGTGACTGA+GGG - chr8_4:1819923-1819942 Msa1306880:five_prime_UTR 45.0%
GACTGAGGGGTGAAAAGAAA+TGG - chr8_4:1819937-1819956 Msa1306880:five_prime_UTR 45.0%
CTGAGGGGTGAAAAGAAATG+GGG - chr8_4:1819939-1819958 Msa1306880:five_prime_UTR 45.0%
GAAAAGAAATGGGGTGGGAA+TGG - chr8_4:1819948-1819967 Msa1306880:five_prime_UTR 45.0%
GACATGCTGGACTGCTATAG+AGG + chr8_4:1818819-1818838 None:intergenic 50.0%
CTGATCGTGTCTTGGCCTAA+CGG - chr8_4:1818863-1818882 Msa1306880:intron 50.0%
!! AATCGGTTGGCCTGCTATAG+AGG - chr8_4:1818900-1818919 Msa1306880:CDS 50.0%
TTGGCCTGCTATAGAGGATC+CGG - chr8_4:1818906-1818925 Msa1306880:CDS 50.0%
AGGCTCGCTATCTTCACTTC+TGG + chr8_4:1818990-1819009 None:intergenic 50.0%
AGATTAGGTCAGGCCTTCCT+TGG + chr8_4:1819025-1819044 None:intergenic 50.0%
CCTCTAGCAAACAAGCCTGA+AGG + chr8_4:1819380-1819399 None:intergenic 50.0%
! CCTTCAGGCTTGTTTGCTAG+AGG - chr8_4:1819377-1819396 Msa1306880:intron 50.0%
! TGCCTTGGTTCTTGCTGAGT+AGG + chr8_4:1819405-1819424 None:intergenic 50.0%
! CTGTTAGGAGTGTGACTGAG+GGG - chr8_4:1819924-1819943 Msa1306880:five_prime_UTR 50.0%
AGGGGTGAAAAGAAATGGGG+TGG - chr8_4:1819942-1819961 Msa1306880:five_prime_UTR 50.0%
GGGGTGAAAAGAAATGGGGT+GGG - chr8_4:1819943-1819962 Msa1306880:five_prime_UTR 50.0%
GACATGCTGGACTGCTATAG+AGG + chr8_4:1818819-1818838 None:intergenic 50.0%
CTGATCGTGTCTTGGCCTAA+CGG - chr8_4:1818863-1818882 Msa1306880:intron 50.0%
!! AATCGGTTGGCCTGCTATAG+AGG - chr8_4:1818900-1818919 Msa1306880:CDS 50.0%
TTGGCCTGCTATAGAGGATC+CGG - chr8_4:1818906-1818925 Msa1306880:CDS 50.0%
AGGCTCGCTATCTTCACTTC+TGG + chr8_4:1818990-1819009 None:intergenic 50.0%
AGATTAGGTCAGGCCTTCCT+TGG + chr8_4:1819025-1819044 None:intergenic 50.0%
CCTCTAGCAAACAAGCCTGA+AGG + chr8_4:1819380-1819399 None:intergenic 50.0%
! CCTTCAGGCTTGTTTGCTAG+AGG - chr8_4:1819377-1819396 Msa1306880:intron 50.0%
! TGCCTTGGTTCTTGCTGAGT+AGG + chr8_4:1819405-1819424 None:intergenic 50.0%
! CTGTTAGGAGTGTGACTGAG+GGG - chr8_4:1819924-1819943 Msa1306880:five_prime_UTR 50.0%
AGGGGTGAAAAGAAATGGGG+TGG - chr8_4:1819942-1819961 Msa1306880:five_prime_UTR 50.0%
GGGGTGAAAAGAAATGGGGT+GGG - chr8_4:1819943-1819962 Msa1306880:five_prime_UTR 50.0%
GTGGATGTGTCTGCTGTTGG+AGG - chr8_4:1818599-1818618 Msa1306880:intron 55.0%
GCTGGACTGCTATAGAGGTG+AGG + chr8_4:1818814-1818833 None:intergenic 55.0%
CGTTAGGCCAAGACACGATC+AGG + chr8_4:1818865-1818884 None:intergenic 55.0%
TGGCCTGCTATAGAGGATCC+GGG - chr8_4:1818907-1818926 Msa1306880:CDS 55.0%
GAACCCGGATCCTCTATAGC+AGG + chr8_4:1818913-1818932 None:intergenic 55.0%
GGGAACTTATAGCCCACAGC+AGG - chr8_4:1819319-1819338 Msa1306880:intron 55.0%
GTGGATGTGTCTGCTGTTGG+AGG - chr8_4:1818599-1818618 Msa1306880:intron 55.0%
GCTGGACTGCTATAGAGGTG+AGG + chr8_4:1818814-1818833 None:intergenic 55.0%
CGTTAGGCCAAGACACGATC+AGG + chr8_4:1818865-1818884 None:intergenic 55.0%
TGGCCTGCTATAGAGGATCC+GGG - chr8_4:1818907-1818926 Msa1306880:CDS 55.0%
GAACCCGGATCCTCTATAGC+AGG + chr8_4:1818913-1818932 None:intergenic 55.0%
GGGAACTTATAGCCCACAGC+AGG - chr8_4:1819319-1819338 Msa1306880:intron 55.0%
GGGTGGGAATGGACCCCATG+TGG - chr8_4:1819959-1819978 Msa1306880:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 1818142 1819981 1818142 ID=Msa1306880;Name=Msa1306880
chr8_4 mRNA 1818142 1819981 1818142 ID=Msa1306880-mRNA-1;Parent=Msa1306880;Name=Msa1306880-mRNA-1;_AED=0.11;_eAED=0.11;_QI=95|0.66|0.75|1|0.66|0.5|4|269|205
chr8_4 exon 1818142 1818491 1818142 ID=Msa1306880-mRNA-1:exon:641;Parent=Msa1306880-mRNA-1
chr8_4 exon 1818900 1819147 1818900 ID=Msa1306880-mRNA-1:exon:640;Parent=Msa1306880-mRNA-1
chr8_4 exon 1819503 1819569 1819503 ID=Msa1306880-mRNA-1:exon:639;Parent=Msa1306880-mRNA-1
chr8_4 exon 1819665 1819981 1819665 ID=Msa1306880-mRNA-1:exon:638;Parent=Msa1306880-mRNA-1
chr8_4 five_prime_UTR 1819887 1819981 1819887 ID=Msa1306880-mRNA-1:five_prime_utr;Parent=Msa1306880-mRNA-1
chr8_4 CDS 1819665 1819886 1819665 ID=Msa1306880-mRNA-1:cds;Parent=Msa1306880-mRNA-1
chr8_4 CDS 1819503 1819569 1819503 ID=Msa1306880-mRNA-1:cds;Parent=Msa1306880-mRNA-1
chr8_4 CDS 1818900 1819147 1818900 ID=Msa1306880-mRNA-1:cds;Parent=Msa1306880-mRNA-1
chr8_4 CDS 1818411 1818491 1818411 ID=Msa1306880-mRNA-1:cds;Parent=Msa1306880-mRNA-1
chr8_4 three_prime_UTR 1818142 1818410 1818142 ID=Msa1306880-mRNA-1:three_prime_utr;Parent=Msa1306880-mRNA-1
Gene Sequence

>Msa1306880

ATGTCTGCTGCTGTAGGAGCTGTGTCTACAACCAAAGATGTTTCCTTCAATGCTCATTTGGAGGAAGAGCACAAAAACAAAGGAACCAACAAGGTTGGTGTCAACTATGATGATGCTGAGGAAATTGTGCCAGATGAAGATGAAGAGGAAGAGGAAGAACCTAAGTTGGAATTGGATCTTGGCCCTCAGTTTTCCCTTAAAGAACAGCTTGAGAAAGATAAAGATGATGAAAGCTTGAGGAAATGGAAAGAGCAACTTCTAGGAAATGTGGATGTGTCTGCTGTTGGAGAGAAAATAGAGCCAGAAGTGAAGATAGCGAGCCTCACCATTATATGCCAAGGAAGGCCTGACCTAATCTTGCCAATTCCATTCACCGCTGATTCCAAAAAGAGCATGTTCACTCTCAAGGAAGGAAGCCAATACAGGCTAAAATTCTCCTTCACTGTCTCCAACAACATAGTTTCTGGCCTCAAATATACCAATGTTGTTTGGAAAACTGGGGTTAGAGGTGAGACAGAGACCTCAATTTATGTTTCATTTGTAGATGATGATCGCAAATGCTACTTGGATGCTAGTTATAGGTTTGAGATTCAGAAGAATTGGCCTACACCACACTGA
Protein sequence

>Msa1306880

MSAAVGAVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDDAEEIVPDEDEEEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGNVDVSAVGEKIEPEVKIASLTIICQGRPDLILPIPFTADSKKSMFTLKEGSQYRLKFSFTVSNNIVSGLKYTNVVWKTGVRGETETSIYVSFVDDDRKCYLDASYRFEIQKNWPTPH*