AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0586690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0586690 MtrunA17_Chr4g0002531 98.333 240 4 0 1 240 1 240 0.0 495
Msa0586690 MtrunA17_Chr1g0149791 73.103 145 39 0 25 169 15 159 1.68e-79 238
Msa0586690 MtrunA17_Chr3g0137121 57.426 202 77 3 25 225 15 208 2.68e-78 234
Msa0586690 MtrunA17_Chr1g0162711 55.056 178 76 2 16 192 30 204 5.19e-72 220
Msa0586690 MtrunA17_Chr1g0154681 54.595 185 80 2 10 193 25 206 6.40e-70 214
Msa0586690 MtrunA17_Chr5g0399541 57.516 153 65 0 19 171 15 167 1.32e-66 205
Msa0586690 MtrunA17_Chr4g0055821 56.643 143 62 0 29 171 24 166 7.19e-63 196
Msa0586690 MtrunA17_Chr4g0063261 48.718 156 78 1 29 182 31 186 5.13e-56 178
Msa0586690 MtrunA17_Chr2g0306871 44.751 181 87 3 34 201 33 213 5.72e-56 178
Msa0586690 MtrunA17_Chr1g0202701 37.589 141 69 5 29 153 4 141 5.22e-24 95.9
Msa0586690 MtrunA17_Chr6g0468771 37.143 140 72 5 29 153 4 142 1.57e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0586690 AT1G80690.1 68.852 183 52 4 12 193 1 179 9.96e-90 265
Msa0586690 AT1G80690.2 67.380 187 52 5 12 193 1 183 2.04e-87 259
Msa0586690 AT5G25170.1 67.073 164 54 0 25 188 16 179 7.52e-81 241
Msa0586690 AT2G25190.1 68.944 161 47 2 12 170 1 160 3.47e-79 238
Msa0586690 AT4G31980.1 65.625 160 54 1 12 170 1 160 4.12e-72 233
Msa0586690 AT1G47740.1 54.396 182 79 1 10 191 52 229 1.31e-70 218
Msa0586690 AT1G47740.2 54.396 182 79 1 10 191 52 229 1.31e-70 218
Msa0586690 AT1G47740.3 54.396 182 79 1 10 191 52 229 1.31e-70 218
Msa0586690 AT5G47310.1 53.793 145 67 0 27 171 28 172 5.57e-63 197
Msa0586690 AT4G17486.1 47.904 167 87 0 28 194 27 193 3.40e-62 194
Msa0586690 AT5G25170.2 62.687 134 50 0 55 188 2 135 4.75e-58 182
Msa0586690 AT4G17486.2 54.400 125 57 0 28 152 27 151 4.29e-54 173
Msa0586690 AT4G25660.1 32.759 174 111 4 49 220 38 207 3.16e-22 92.0
Msa0586690 AT4G25680.1 33.846 195 113 8 49 234 38 225 1.07e-21 90.9
Msa0586690 AT3G07090.2 33.077 130 69 5 29 149 8 128 1.55e-11 63.2
Msa0586690 AT3G07090.1 33.077 130 69 5 29 149 8 128 1.55e-11 63.2

Find 57 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCAATATGTTGCCTTTCT+TGG 0.190798 4_2:-8017766 None:intergenic
GGTCTTGGTGTTTATCATTC+TGG 0.204257 4_2:+8014483 Msa0586690:CDS
GATAGCAACTATTGTAAAAT+TGG 0.213801 4_2:-8017737 None:intergenic
CTGAACCCTTCACATTTCTT+TGG 0.240921 4_2:-8015399 None:intergenic
AATTCAACGGTTATGCTTAC+TGG 0.242093 4_2:+8014457 Msa0586690:CDS
CTTGAAACATTAACCTTGTT+TGG 0.254573 4_2:-8017553 None:intergenic
GAAATAGTGCAGTTGAATTA+TGG 0.270889 4_2:+8014326 Msa0586690:exon
AACGGTTATGCTTACTGGTT+TGG 0.341414 4_2:+8014462 Msa0586690:CDS
TATGCTTACTGGTTTGGTCT+TGG 0.389422 4_2:+8014468 Msa0586690:CDS
CTGGAAATTCTATACCTAGT+TGG 0.392597 4_2:+8015577 Msa0586690:CDS
TCACCGGCTTCTTCTAGCTC+TGG 0.400392 4_2:+8017598 Msa0586690:CDS
TGGAAATTCTATACCTAGTT+GGG 0.400569 4_2:+8015578 Msa0586690:CDS
AACCAAGAAAGGCAACATAT+TGG 0.410635 4_2:+8017764 Msa0586690:three_prime_UTR
TTTAGAAAGACAATAATGAT+TGG 0.412372 4_2:+8015423 Msa0586690:CDS
TCACGAAACGGTGTCGTATC+GGG 0.415311 4_2:+8014396 Msa0586690:CDS
GTTAATCGTCTAGCTAGAAT+TGG 0.428771 4_2:+8015600 Msa0586690:CDS
CATGGTGTTGAATATGCATT+TGG 0.429052 4_2:+8015336 Msa0586690:CDS
GTTTATCATTCTGGTGTTCA+AGG 0.430967 4_2:+8014492 Msa0586690:CDS
TATTAATTTGTTCAAGAGAT+GGG 0.448603 4_2:+8017894 Msa0586690:three_prime_UTR
TTCACGAAACGGTGTCGTAT+CGG 0.456858 4_2:+8014395 Msa0586690:CDS
TGGTTCTCCTTCAAATATAC+CGG 0.461685 4_2:-8015379 None:intergenic
TGTCGTATCGGGATCGGTGC+CGG 0.473134 4_2:+8014407 Msa0586690:CDS
TGCTATCAACAAACCAAGAA+AGG 0.486381 4_2:+8017753 Msa0586690:three_prime_UTR
CTTCTAGCTCTGGATTAAGA+AGG 0.486970 4_2:+8017608 Msa0586690:CDS
GCACATGAGTATGCATCAAC+CGG 0.506681 4_2:+8015360 Msa0586690:CDS
TTCTAGCTCTGGATTAAGAA+GGG 0.509805 4_2:+8017609 Msa0586690:CDS
GATGCTTGTGTTAAGCTCAC+TGG 0.515807 4_2:+8015558 Msa0586690:CDS
GCATCAACCGGTATATTTGA+AGG 0.517962 4_2:+8015372 Msa0586690:CDS
TTATTAATTTGTTCAAGAGA+TGG 0.520404 4_2:+8017893 Msa0586690:three_prime_UTR
TTTGTTGAGTTCAATGTCAC+GGG 0.532965 4_2:-8017451 None:intergenic
ACCTTCGAGAGCATCTTCAC+CGG 0.544046 4_2:+8017582 Msa0586690:CDS
TGTGGAAAAGTTTCACGAAA+CGG 0.553507 4_2:+8014384 Msa0586690:CDS
ATTAATTTGTTCAAGAGATG+GGG 0.555388 4_2:+8017895 Msa0586690:three_prime_UTR
CCGGCAAAACCCGCCGCCGG+TGG 0.563524 4_2:+8014133 Msa0586690:five_prime_UTR
GGAGAACCAAAGAAATGTGA+AGG 0.567060 4_2:+8015393 Msa0586690:CDS
GCAATAATCAAAGGTGAAGG+TGG 0.569859 4_2:-8017655 None:intergenic
AAACGGTGTCGTATCGGGAT+CGG 0.582657 4_2:+8014401 Msa0586690:CDS
ACACCGGCAAAACCCGCCGC+CGG 0.585578 4_2:+8014130 Msa0586690:five_prime_UTR
GATTAAGAAGGGGAAGGAGT+AGG 0.587384 4_2:+8017620 Msa0586690:CDS
TCTAGCTCTGGATTAAGAAG+GGG 0.590101 4_2:+8017610 Msa0586690:CDS
TGGTGAAGTGAGAAGTGTGA+TGG 0.594058 4_2:+8015464 Msa0586690:CDS
GCCGGTGAAGATGCTCTCGA+AGG 0.607232 4_2:-8017583 None:intergenic
CGTCGGAGAATGTTACTATG+TGG 0.607728 4_2:+8014366 Msa0586690:CDS
TAAACATCAAGATGAAGGGG+AGG 0.607838 4_2:-8017677 None:intergenic
GCTAGACGATTAACCCAACT+AGG 0.614536 4_2:-8015591 None:intergenic
ATAATCAAAGGTGAAGGTGG+AGG 0.615233 4_2:-8017652 None:intergenic
AATCCAGAGCTAGAAGAAGC+CGG 0.616375 4_2:-8017601 None:intergenic
ATTAACAAGTGAACCAAACA+AGG 0.618419 4_2:+8017540 Msa0586690:CDS
GAACTTGCTGCTGAGTACAA+AGG 0.625237 4_2:+8015489 Msa0586690:CDS
GAAGGGGAGGCAATAATCAA+AGG 0.625643 4_2:-8017664 None:intergenic
CATAACACAAAACTTTACAC+CGG 0.627597 4_2:+8014114 Msa0586690:five_prime_UTR
AAGAGAAGAAAACACATCGT+CGG 0.636912 4_2:+8014349 Msa0586690:CDS
TACGATCTGACACAATTCAA+CGG 0.645029 4_2:+8014444 Msa0586690:CDS
GAGAACCAAAGAAATGTGAA+GGG 0.652920 4_2:+8015394 Msa0586690:CDS
GAGGCAATAATCAAAGGTGA+AGG 0.662473 4_2:-8017658 None:intergenic
TCGTACACGTTAAGATACAC+CGG 0.689501 4_2:-8014426 None:intergenic
GCTCTGGATTAAGAAGGGGA+AGG 0.690627 4_2:+8017614 Msa0586690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTTTTA+AGG + chr4_2:8016006-8016025 Msa0586690:intron 0.0%
!! TCTTAAATATAAGTAAAAAT+TGG - chr4_2:8014884-8014903 None:intergenic 10.0%
!!! CATTTCTATTTTTATTTTTT+TGG + chr4_2:8015221-8015240 Msa0586690:intron 10.0%
!!! TATTTTTATTTTTTTGGTTT+GGG + chr4_2:8015227-8015246 Msa0586690:intron 10.0%
!! AATAAATTGAAATAATTAGT+TGG + chr4_2:8015724-8015743 Msa0586690:intron 10.0%
!! ATAAATTGAAATAATTAGTT+GGG + chr4_2:8015725-8015744 Msa0586690:intron 10.0%
!!! TTTACATTTAATTTAAAGAA+AGG + chr4_2:8016869-8016888 Msa0586690:intron 10.0%
!!! TATTAATTTTAAAATGCATA+AGG + chr4_2:8016906-8016925 Msa0586690:intron 10.0%
!! TTTATATGCATTTAAATATA+TGG + chr4_2:8017010-8017029 Msa0586690:intron 10.0%
!! AAAAAATGTATAATAAAAGT+AGG + chr4_2:8017292-8017311 Msa0586690:intron 10.0%
!! ATTTCCAATAAAAAAATGAT+GGG - chr4_2:8014193-8014212 None:intergenic 15.0%
!! AATTTCCAATAAAAAAATGA+TGG - chr4_2:8014194-8014213 None:intergenic 15.0%
!! ATTTCCAATAAAAAAATGAT+GGG - chr4_2:8014193-8014212 None:intergenic 15.0%
!! AATTTCCAATAAAAAAATGA+TGG - chr4_2:8014194-8014213 None:intergenic 15.0%
!! AAAATTTGAATCAAATACAT+CGG + chr4_2:8014847-8014866 Msa0586690:intron 15.0%
!!! TTTTTACTTATATTTAAGAC+CGG + chr4_2:8014885-8014904 Msa0586690:intron 15.0%
!!! TTTGTTAAAGACTTTTTTTT+AGG + chr4_2:8015179-8015198 Msa0586690:intron 15.0%
!!! TTGTTAAAGACTTTTTTTTA+GGG + chr4_2:8015180-8015199 Msa0586690:intron 15.0%
!!! CTATTTTTATTTTTTTGGTT+TGG + chr4_2:8015226-8015245 Msa0586690:intron 15.0%
!!! ATTTCAATTTATTTGTTTCT+TGG - chr4_2:8015717-8015736 None:intergenic 15.0%
!!! ATTATGTTTTAATTGATACA+CGG - chr4_2:8015833-8015852 None:intergenic 15.0%
!!! TCAAAAAATGTATTTTTTCT+TGG + chr4_2:8016379-8016398 Msa0586690:intron 15.0%
!!! CAAAAAATGTATTTTTTCTT+GGG + chr4_2:8016380-8016399 Msa0586690:intron 15.0%
!!! AAAAGTATTTTACAAGTATT+AGG - chr4_2:8016806-8016825 None:intergenic 15.0%
!! CTTTGTAACAAAAAAAAAAT+AGG + chr4_2:8017262-8017281 Msa0586690:intron 15.0%
!!! TTTGAATGTGAAGTTTTTTT+TGG - chr4_2:8014162-8014181 None:intergenic 20.0%
!! TTCGGAAAAATTAAGAATTA+AGG - chr4_2:8014547-8014566 None:intergenic 20.0%
!! AGACTAGAATAATATTAATC+CGG - chr4_2:8014907-8014926 None:intergenic 20.0%
!! TTATTAGTACTTAGATTAGT+AGG + chr4_2:8015033-8015052 Msa0586690:intron 20.0%
!! TATTAGTACTTAGATTAGTA+GGG + chr4_2:8015034-8015053 Msa0586690:intron 20.0%
!!! GATTGATATTTGAAATTTTG+AGG + chr4_2:8015136-8015155 Msa0586690:intron 20.0%
!!! TATTTTGTTTTTTCAGTTCA+TGG + chr4_2:8015318-8015337 Msa0586690:intron 20.0%
!! TTTAGAAAGACAATAATGAT+TGG + chr4_2:8015423-8015442 Msa0586690:CDS 20.0%
!!! ACAATCTTTTGATAAGTTTT+AGG + chr4_2:8016050-8016069 Msa0586690:intron 20.0%
!! AATATCTACGAAACAAAAAT+TGG + chr4_2:8016194-8016213 Msa0586690:intron 20.0%
!!! ATTTACATTTGCTATAAATG+TGG + chr4_2:8016264-8016283 Msa0586690:intron 20.0%
!!! GATCAAGTCAAATTTTTTAT+TGG + chr4_2:8016331-8016350 Msa0586690:intron 20.0%
!! TAAAAATTACTAATCTACGT+TGG + chr4_2:8016439-8016458 Msa0586690:intron 20.0%
!! TAAAATAATACAACCAACAA+TGG - chr4_2:8016559-8016578 None:intergenic 20.0%
!! AAAATGTGAATAAATGATGT+GGG + chr4_2:8016718-8016737 Msa0586690:intron 20.0%
!!! TAATTTTAAAATGCATAAGG+TGG + chr4_2:8016909-8016928 Msa0586690:intron 20.0%
!!! ATTTTAAACATCAAGATGAA+GGG - chr4_2:8017684-8017703 None:intergenic 20.0%
!!! TTATTAATTTGTTCAAGAGA+TGG + chr4_2:8017893-8017912 Msa0586690:three_prime_UTR 20.0%
!!! TATTAATTTGTTCAAGAGAT+GGG + chr4_2:8017894-8017913 Msa0586690:three_prime_UTR 20.0%
!!! TATGCCCATCATTTTTTTAT+TGG + chr4_2:8014218-8014237 Msa0586690:five_prime_UTR 25.0%
! TTTACTCAACGTTGAATTTA+CGG + chr4_2:8014652-8014671 Msa0586690:intron 25.0%
!! CTTTAAAGATTTCAAATGCA+CGG + chr4_2:8014804-8014823 Msa0586690:intron 25.0%
!! AATCAAATACATCGGATTTT+TGG + chr4_2:8014855-8014874 Msa0586690:intron 25.0%
! ATTTCGATTTCTAAACGAAA+TGG - chr4_2:8015061-8015080 None:intergenic 25.0%
! AATGATTGGAAAAACAGATA+TGG + chr4_2:8015437-8015456 Msa0586690:CDS 25.0%
!! GGAACATGTATTTTGTAATT+TGG - chr4_2:8015812-8015831 None:intergenic 25.0%
! TCAAAGATATTGAAAGTCAA+TGG - chr4_2:8015893-8015912 None:intergenic 25.0%
!! ATTGTTGACTAAAGCAAATT+CGG + chr4_2:8015929-8015948 Msa0586690:intron 25.0%
!!! ATAAGTTTTAGGCTGAATTT+AGG + chr4_2:8016061-8016080 Msa0586690:intron 25.0%
!!! TAAGTTTTAGGCTGAATTTA+GGG + chr4_2:8016062-8016081 Msa0586690:intron 25.0%
!!! GAGATTATAGTTGATAATGA+TGG + chr4_2:8016296-8016315 Msa0586690:intron 25.0%
! GAAAATGTGAATAAATGATG+TGG + chr4_2:8016717-8016736 Msa0586690:intron 25.0%
! TATACGCAATTAAATGACAT+TGG + chr4_2:8016773-8016792 Msa0586690:intron 25.0%
! TTCCCATGTATAAAAAAATG+TGG + chr4_2:8017108-8017127 Msa0586690:intron 25.0%
! TCCCATGTATAAAAAAATGT+GGG + chr4_2:8017109-8017128 Msa0586690:intron 25.0%
!!! TCCCACATTTTTTTATACAT+GGG - chr4_2:8017113-8017132 None:intergenic 25.0%
!!! TTCCCACATTTTTTTATACA+TGG - chr4_2:8017114-8017133 None:intergenic 25.0%
! TCTTGTACGTACAAATTATA+TGG + chr4_2:8017139-8017158 Msa0586690:intron 25.0%
! AAGCAATGTTTATCTCAATA+AGG - chr4_2:8017396-8017415 None:intergenic 25.0%
! AACATTGCTTATATTATTGC+AGG + chr4_2:8017406-8017425 Msa0586690:intron 25.0%
!! TTTTAAACATCAAGATGAAG+GGG - chr4_2:8017683-8017702 None:intergenic 25.0%
!! GATTTTAAACATCAAGATGA+AGG - chr4_2:8017685-8017704 None:intergenic 25.0%
! GATAGCAACTATTGTAAAAT+TGG - chr4_2:8017740-8017759 None:intergenic 25.0%
! AAGATATCAAAAAACAAGCA+AGG + chr4_2:8017850-8017869 Msa0586690:three_prime_UTR 25.0%
!!! ATTAATTTGTTCAAGAGATG+GGG + chr4_2:8017895-8017914 Msa0586690:three_prime_UTR 25.0%
CATAACACAAAACTTTACAC+CGG + chr4_2:8014114-8014133 Msa0586690:five_prime_UTR 30.0%
!!! AAGTTTTTTTTGGATTCCAC+CGG - chr4_2:8014152-8014171 None:intergenic 30.0%
!!! CATGCCCATCATTTTTTTAT+TGG + chr4_2:8014186-8014205 Msa0586690:five_prime_UTR 30.0%
!! GTTCTTCACTTTTCTCAATT+GGG - chr4_2:8014255-8014274 None:intergenic 30.0%
!! AGTTCTTCACTTTTCTCAAT+TGG - chr4_2:8014256-8014275 None:intergenic 30.0%
GAAATAGTGCAGTTGAATTA+TGG + chr4_2:8014326-8014345 Msa0586690:exon 30.0%
! GATTAGATCTAAAGCAAGTT+CGG - chr4_2:8014565-8014584 None:intergenic 30.0%
! ACTTGCTTTAGATCTAATCT+AGG + chr4_2:8014566-8014585 Msa0586690:intron 30.0%
TAGTCTCGAAAAACTTTGTT+GGG + chr4_2:8014921-8014940 Msa0586690:intron 30.0%
TGGAAATTCTATACCTAGTT+GGG + chr4_2:8015578-8015597 Msa0586690:CDS 30.0%
GAATAGTAGTAACAACTAGT+AGG - chr4_2:8015639-8015658 None:intergenic 30.0%
!!! TCTTGCTTTTTTTCCTGTTT+TGG + chr4_2:8015668-8015687 Msa0586690:intron 30.0%
ACATGATCAATATCCAAAAC+AGG - chr4_2:8015684-8015703 None:intergenic 30.0%
!! TGTTTTGGATATTGATCATG+TGG + chr4_2:8015683-8015702 Msa0586690:intron 30.0%
AATAATTAGTTGGGTGAAAG+TGG + chr4_2:8015734-8015753 Msa0586690:intron 30.0%
ATAATTAGTTGGGTGAAAGT+GGG + chr4_2:8015735-8015754 Msa0586690:intron 30.0%
! TTGTTGACTAAAGCAAATTC+GGG + chr4_2:8015930-8015949 Msa0586690:intron 30.0%
TAATGAGTTTCTGTTTGACT+AGG + chr4_2:8015961-8015980 Msa0586690:intron 30.0%
CTTGATCAAAATCTTCACAA+CGG - chr4_2:8016236-8016255 None:intergenic 30.0%
TAATACAACCAACAATGGAT+TGG - chr4_2:8016554-8016573 None:intergenic 30.0%
ACATATCAACTGCTAAGAAA+TGG - chr4_2:8017220-8017239 None:intergenic 30.0%
! GAACTTTTATGCTAAACTTC+CGG + chr4_2:8017353-8017372 Msa0586690:intron 30.0%
GGTGATAGATATCTTAATTC+CGG - chr4_2:8017375-8017394 None:intergenic 30.0%
! TTTTGTTGAGTTCAATGTCA+CGG - chr4_2:8017455-8017474 None:intergenic 30.0%
ATTAACAAGTGAACCAAACA+AGG + chr4_2:8017540-8017559 Msa0586690:CDS 30.0%
CTTGAAACATTAACCTTGTT+TGG - chr4_2:8017556-8017575 None:intergenic 30.0%
AAAAAACAAGCAAGGAATCA+TGG + chr4_2:8017858-8017877 Msa0586690:three_prime_UTR 30.0%
AAGAGAAGAAAACACATCGT+CGG + chr4_2:8014349-8014368 Msa0586690:CDS 35.0%
TGTGGAAAAGTTTCACGAAA+CGG + chr4_2:8014384-8014403 Msa0586690:CDS 35.0%
TACGATCTGACACAATTCAA+CGG + chr4_2:8014444-8014463 Msa0586690:CDS 35.0%
AATTCAACGGTTATGCTTAC+TGG + chr4_2:8014457-8014476 Msa0586690:CDS 35.0%
GTTTATCATTCTGGTGTTCA+AGG + chr4_2:8014492-8014511 Msa0586690:CDS 35.0%
! GATCTAATCTAGGATCTATG+TGG + chr4_2:8014576-8014595 Msa0586690:intron 35.0%
! TCTCTCTCTTTCTAAAAGTG+TGG + chr4_2:8014618-8014637 Msa0586690:intron 35.0%
CTAGTCTCGAAAAACTTTGT+TGG + chr4_2:8014920-8014939 Msa0586690:intron 35.0%
CTTTGTTGGGAATAACCAAA+TGG + chr4_2:8014934-8014953 Msa0586690:intron 35.0%
AGCTAGAATTCATCACCATT+TGG - chr4_2:8014952-8014971 None:intergenic 35.0%
! TGGTGATGAATTCTAGCTAA+AGG + chr4_2:8014954-8014973 Msa0586690:intron 35.0%
TCTAGCTAAAGGTGAATCAT+TGG + chr4_2:8014965-8014984 Msa0586690:intron 35.0%
! CATGGTGTTGAATATGCATT+TGG + chr4_2:8015336-8015355 Msa0586690:CDS 35.0%
TGGTTCTCCTTCAAATATAC+CGG - chr4_2:8015382-8015401 None:intergenic 35.0%
GAGAACCAAAGAAATGTGAA+GGG + chr4_2:8015394-8015413 Msa0586690:CDS 35.0%
GGAAAAACAGATATGGAACT+TGG + chr4_2:8015444-8015463 Msa0586690:CDS 35.0%
CACAAGCATCATTGCAAAAA+TGG - chr4_2:8015548-8015567 None:intergenic 35.0%
CTGGAAATTCTATACCTAGT+TGG + chr4_2:8015577-8015596 Msa0586690:CDS 35.0%
GTTAATCGTCTAGCTAGAAT+TGG + chr4_2:8015600-8015619 Msa0586690:CDS 35.0%
AATCTCAACACAACAACCAT+GGG - chr4_2:8015782-8015801 None:intergenic 35.0%
AAATCTCAACACAACAACCA+TGG - chr4_2:8015783-8015802 None:intergenic 35.0%
GTTGTTGTGTTGAGATTTGA+CGG + chr4_2:8015785-8015804 Msa0586690:intron 35.0%
TCTTTGATTGCGTAAATGTG+TGG + chr4_2:8015906-8015925 Msa0586690:intron 35.0%
! GGGATATAAGAACCTTTTCT+CGG + chr4_2:8016082-8016101 Msa0586690:intron 35.0%
! TAAGAACCTTTTCTCGGAAT+AGG + chr4_2:8016088-8016107 Msa0586690:intron 35.0%
AACTTTCCTATTCCGAGAAA+AGG - chr4_2:8016097-8016116 None:intergenic 35.0%
AATCCTTAAACACGTGATCA+GGG - chr4_2:8016510-8016529 None:intergenic 35.0%
TAATCCTTAAACACGTGATC+AGG - chr4_2:8016511-8016530 None:intergenic 35.0%
!! CAACAATGGATTGGTCTAAA+TGG - chr4_2:8016545-8016564 None:intergenic 35.0%
CATTTAGACCAATCCATTGT+TGG + chr4_2:8016543-8016562 Msa0586690:intron 35.0%
ATCAAAATCCTCAAGACCAT+TGG + chr4_2:8016644-8016663 Msa0586690:intron 35.0%
ATTAGGCAAATAGTCTCCAA+TGG - chr4_2:8016663-8016682 None:intergenic 35.0%
CAAATTCAACGTGTAGCATT+AGG - chr4_2:8016680-8016699 None:intergenic 35.0%
! TGCTACACGTTGAATTTGTT+AGG + chr4_2:8016682-8016701 Msa0586690:intron 35.0%
!! GGGAGAGTTACTTTAGAATT+AGG + chr4_2:8016738-8016757 Msa0586690:intron 35.0%
! TTCTTAGCAGTTGATATGTG+TGG + chr4_2:8017221-8017240 Msa0586690:intron 35.0%
TTTGTTGAGTTCAATGTCAC+GGG - chr4_2:8017454-8017473 None:intergenic 35.0%
!! TTCTAGCTCTGGATTAAGAA+GGG + chr4_2:8017609-8017628 Msa0586690:CDS 35.0%
TGCTATCAACAAACCAAGAA+AGG + chr4_2:8017753-8017772 Msa0586690:three_prime_UTR 35.0%
AACCAAGAAAGGCAACATAT+TGG + chr4_2:8017764-8017783 Msa0586690:three_prime_UTR 35.0%
ATCCAATATGTTGCCTTTCT+TGG - chr4_2:8017769-8017788 None:intergenic 35.0%
!!! TTTTTTTTGGATTCCACCGG+CGG - chr4_2:8014149-8014168 None:intergenic 40.0%
TCGTACACGTTAAGATACAC+CGG - chr4_2:8014429-8014448 None:intergenic 40.0%
! AACGGTTATGCTTACTGGTT+TGG + chr4_2:8014462-8014481 Msa0586690:CDS 40.0%
!! TATGCTTACTGGTTTGGTCT+TGG + chr4_2:8014468-8014487 Msa0586690:CDS 40.0%
!! GGTCTTGGTGTTTATCATTC+TGG + chr4_2:8014483-8014502 Msa0586690:CDS 40.0%
GCATCAACCGGTATATTTGA+AGG + chr4_2:8015372-8015391 Msa0586690:CDS 40.0%
GGAGAACCAAAGAAATGTGA+AGG + chr4_2:8015393-8015412 Msa0586690:CDS 40.0%
CTGAACCCTTCACATTTCTT+TGG - chr4_2:8015402-8015421 None:intergenic 40.0%
ATCCTTAAACACGTGATCAG+GGG - chr4_2:8016509-8016528 None:intergenic 40.0%
! AATAGTCTCCAATGGTCTTG+AGG - chr4_2:8016655-8016674 None:intergenic 40.0%
!! CTTCTAGCTCTGGATTAAGA+AGG + chr4_2:8017608-8017627 Msa0586690:CDS 40.0%
!! TCTAGCTCTGGATTAAGAAG+GGG + chr4_2:8017610-8017629 Msa0586690:CDS 40.0%
ATAATCAAAGGTGAAGGTGG+AGG - chr4_2:8017655-8017674 None:intergenic 40.0%
GCAATAATCAAAGGTGAAGG+TGG - chr4_2:8017658-8017677 None:intergenic 40.0%
GAGGCAATAATCAAAGGTGA+AGG - chr4_2:8017661-8017680 None:intergenic 40.0%
TAAACATCAAGATGAAGGGG+AGG - chr4_2:8017680-8017699 None:intergenic 40.0%
CGTCGGAGAATGTTACTATG+TGG + chr4_2:8014366-8014385 Msa0586690:CDS 45.0%
!! TTCACGAAACGGTGTCGTAT+CGG + chr4_2:8014395-8014414 Msa0586690:CDS 45.0%
GCACATGAGTATGCATCAAC+CGG + chr4_2:8015360-8015379 Msa0586690:CDS 45.0%
! TGGTGAAGTGAGAAGTGTGA+TGG + chr4_2:8015464-8015483 Msa0586690:CDS 45.0%
!! GAACTTGCTGCTGAGTACAA+AGG + chr4_2:8015489-8015508 Msa0586690:CDS 45.0%
GATGCTTGTGTTAAGCTCAC+TGG + chr4_2:8015558-8015577 Msa0586690:CDS 45.0%
GCTAGACGATTAACCCAACT+AGG - chr4_2:8015594-8015613 None:intergenic 45.0%
AAGTGGGAGCAATATGCAAG+CGG + chr4_2:8015751-8015770 Msa0586690:intron 45.0%
AAGTCAATGGATCGAGAACG+AGG - chr4_2:8015880-8015899 None:intergenic 45.0%
! AACACGTGATCAGGGGTTTT+TGG - chr4_2:8016502-8016521 None:intergenic 45.0%
AACCCCTGATCACGTGTTTA+AGG + chr4_2:8016504-8016523 Msa0586690:intron 45.0%
ACATTTGTCATGCCTGACGT+AGG + chr4_2:8017177-8017196 Msa0586690:intron 45.0%
TGCAAGTTCAAACCTACGTC+AGG - chr4_2:8017192-8017211 None:intergenic 45.0%
AATCCAGAGCTAGAAGAAGC+CGG - chr4_2:8017604-8017623 None:intergenic 45.0%
GATTAAGAAGGGGAAGGAGT+AGG + chr4_2:8017620-8017639 Msa0586690:CDS 45.0%
GAAGGGGAGGCAATAATCAA+AGG - chr4_2:8017667-8017686 None:intergenic 45.0%
!!! TCTATTTTTAATATAAAAAA+TGG + chr4_2:8015267-8015286 Msa0586690:intron 5.0%
!!! CTATTTTTAATATAAAAAAT+GGG + chr4_2:8015268-8015287 Msa0586690:intron 5.0%
!! TCACGAAACGGTGTCGTATC+GGG + chr4_2:8014396-8014415 Msa0586690:CDS 50.0%
!! AAACGGTGTCGTATCGGGAT+CGG + chr4_2:8014401-8014420 Msa0586690:CDS 50.0%
ACGTCTTGCTAACGAGTGTC+TGG + chr4_2:8014775-8014794 Msa0586690:intron 50.0%
CGTCTTGCTAACGAGTGTCT+GGG + chr4_2:8014776-8014795 Msa0586690:intron 50.0%
GTCTTGCTAACGAGTGTCTG+GGG + chr4_2:8014777-8014796 Msa0586690:intron 50.0%
AGTGGGAGCAATATGCAAGC+GGG + chr4_2:8015752-8015771 Msa0586690:intron 50.0%
!! ACCTTCGAGAGCATCTTCAC+CGG + chr4_2:8017582-8017601 Msa0586690:CDS 50.0%
! GCTCTGGATTAAGAAGGGGA+AGG + chr4_2:8017614-8017633 Msa0586690:CDS 50.0%
! TTTTTGGATTCCACCGGCGG+CGG - chr4_2:8014146-8014165 None:intergenic 55.0%
GTGGGAGCAATATGCAAGCG+GGG + chr4_2:8015753-8015772 Msa0586690:intron 55.0%
!! TCACCGGCTTCTTCTAGCTC+TGG + chr4_2:8017598-8017617 Msa0586690:CDS 55.0%
! TTTTGGATTCCACCGGCGGC+GGG - chr4_2:8014145-8014164 None:intergenic 60.0%
TGTCGTATCGGGATCGGTGC+CGG + chr4_2:8014407-8014426 Msa0586690:CDS 60.0%
GCCGGTGAAGATGCTCTCGA+AGG - chr4_2:8017586-8017605 None:intergenic 60.0%
TATGCAAGCGGGGTCGCCCA+TGG + chr4_2:8015763-8015782 Msa0586690:intron 65.0%
ACACCGGCAAAACCCGCCGC+CGG + chr4_2:8014130-8014149 Msa0586690:five_prime_UTR 70.0%
!! CCACCGGCGGCGGGTTTTGC+CGG - chr4_2:8014136-8014155 None:intergenic 75.0%
! CCGGCAAAACCCGCCGCCGG+TGG + chr4_2:8014133-8014152 Msa0586690:five_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 8014096 8017922 8014096 ID=Msa0586690;Name=Msa0586690
chr4_2 mRNA 8014096 8017922 8014096 ID=Msa0586690-mRNA-1;Parent=Msa0586690;Name=Msa0586690-mRNA-1;_AED=0.05;_eAED=0.05;_QI=249|1|1|1|1|1|3|229|240
chr4_2 exon 8014096 8014513 8014096 ID=Msa0586690-mRNA-1:exon:2118;Parent=Msa0586690-mRNA-1
chr4_2 exon 8015334 8015621 8015334 ID=Msa0586690-mRNA-1:exon:2119;Parent=Msa0586690-mRNA-1
chr4_2 exon 8017428 8017922 8017428 ID=Msa0586690-mRNA-1:exon:2120;Parent=Msa0586690-mRNA-1
chr4_2 five_prime_UTR 8014096 8014344 8014096 ID=Msa0586690-mRNA-1:five_prime_utr;Parent=Msa0586690-mRNA-1
chr4_2 CDS 8014345 8014513 8014345 ID=Msa0586690-mRNA-1:cds;Parent=Msa0586690-mRNA-1
chr4_2 CDS 8015334 8015621 8015334 ID=Msa0586690-mRNA-1:cds;Parent=Msa0586690-mRNA-1
chr4_2 CDS 8017428 8017693 8017428 ID=Msa0586690-mRNA-1:cds;Parent=Msa0586690-mRNA-1
chr4_2 three_prime_UTR 8017694 8017922 8017694 ID=Msa0586690-mRNA-1:three_prime_utr;Parent=Msa0586690-mRNA-1
Gene Sequence

>Msa0586690

ATGGAAGAGAAGAAAACACATCGTCGGAGAATGTTACTATGTGGAAAAGTTTCACGAAACGGTGTCGTATCGGGATCGGTGCCGGTGTATCTTAACGTGTACGATCTGACACAATTCAACGGTTATGCTTACTGGTTTGGTCTTGGTGTTTATCATTCTGGTGTTCAAGTTCATGGTGTTGAATATGCATTTGGTGCACATGAGTATGCATCAACCGGTATATTTGAAGGAGAACCAAAGAAATGTGAAGGGTTCAGATTTAGAAAGACAATAATGATTGGAAAAACAGATATGGAACTTGGTGAAGTGAGAAGTGTGATGGAAGAACTTGCTGCTGAGTACAAAGGAAATGCTTACAATCTCATTACAAAAAATTGTAACCATTTTTGCAATGATGCTTGTGTTAAGCTCACTGGAAATTCTATACCTAGTTGGGTTAATCGTCTAGCTAGAATTGGTTTTATGTGCAATTGTGTTCTTCCCGTGACATTGAACTCAACAAAAGTTAGACATCATCATCATAGAATAGAAGACAAACAATGTGACGATGATAAACAACTCAAACAAGCATTAACAAGTGAACCAAACAAGGTTAATGTTTCAAGTTCAACACCTTCGAGAGCATCTTCACCGGCTTCTTCTAGCTCTGGATTAAGAAGGGGAAGGAGTAGGACAAGACGTCCTCCACCTTCACCTTTGATTATTGCCTCCCCTTCATCTTGA
Protein sequence

>Msa0586690

MEEKKTHRRRMLLCGKVSRNGVVSGSVPVYLNVYDLTQFNGYAYWFGLGVYHSGVQVHGVEYAFGAHEYASTGIFEGEPKKCEGFRFRKTIMIGKTDMELGEVRSVMEELAAEYKGNAYNLITKNCNHFCNDACVKLTGNSIPSWVNRLARIGFMCNCVLPVTLNSTKVRHHHHRIEDKQCDDDKQLKQALTSEPNKVNVSSSTPSRASSPASSSSGLRRGRSRTRRPPPSPLIIASPSS*