AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0664610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0664610 MtrunA17_Chr4g0002531 98.333 240 4 0 1 240 1 240 0.0 495
Msa0664610 MtrunA17_Chr1g0149791 73.103 145 39 0 25 169 15 159 1.68e-79 238
Msa0664610 MtrunA17_Chr3g0137121 57.426 202 77 3 25 225 15 208 2.68e-78 234
Msa0664610 MtrunA17_Chr1g0162711 55.056 178 76 2 16 192 30 204 5.19e-72 220
Msa0664610 MtrunA17_Chr1g0154681 54.595 185 80 2 10 193 25 206 6.40e-70 214
Msa0664610 MtrunA17_Chr5g0399541 57.516 153 65 0 19 171 15 167 1.32e-66 205
Msa0664610 MtrunA17_Chr4g0055821 56.643 143 62 0 29 171 24 166 7.19e-63 196
Msa0664610 MtrunA17_Chr4g0063261 48.718 156 78 1 29 182 31 186 5.13e-56 178
Msa0664610 MtrunA17_Chr2g0306871 44.751 181 87 3 34 201 33 213 5.72e-56 178
Msa0664610 MtrunA17_Chr1g0202701 37.589 141 69 5 29 153 4 141 5.22e-24 95.9
Msa0664610 MtrunA17_Chr6g0468771 37.143 140 72 5 29 153 4 142 1.57e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0664610 AT1G80690.1 68.852 183 52 4 12 193 1 179 9.96e-90 265
Msa0664610 AT1G80690.2 67.380 187 52 5 12 193 1 183 2.04e-87 259
Msa0664610 AT5G25170.1 67.073 164 54 0 25 188 16 179 7.52e-81 241
Msa0664610 AT2G25190.1 68.944 161 47 2 12 170 1 160 3.47e-79 238
Msa0664610 AT4G31980.1 65.625 160 54 1 12 170 1 160 4.12e-72 233
Msa0664610 AT1G47740.1 54.396 182 79 1 10 191 52 229 1.31e-70 218
Msa0664610 AT1G47740.2 54.396 182 79 1 10 191 52 229 1.31e-70 218
Msa0664610 AT1G47740.3 54.396 182 79 1 10 191 52 229 1.31e-70 218
Msa0664610 AT5G47310.1 53.793 145 67 0 27 171 28 172 5.57e-63 197
Msa0664610 AT4G17486.1 47.904 167 87 0 28 194 27 193 3.40e-62 194
Msa0664610 AT5G25170.2 62.687 134 50 0 55 188 2 135 4.75e-58 182
Msa0664610 AT4G17486.2 54.400 125 57 0 28 152 27 151 4.29e-54 173
Msa0664610 AT4G25660.1 32.759 174 111 4 49 220 38 207 3.16e-22 92.0
Msa0664610 AT4G25680.1 33.846 195 113 8 49 234 38 225 1.07e-21 90.9
Msa0664610 AT3G07090.2 33.077 130 69 5 29 149 8 128 1.55e-11 63.2
Msa0664610 AT3G07090.1 33.077 130 69 5 29 149 8 128 1.55e-11 63.2

Find 57 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCAATATGTTGCCTTTCT+TGG 0.190798 4_4:-3644451 None:intergenic
GGTCTTGGTGTTTATCATTC+TGG 0.204257 4_4:+3641142 Msa0664610:CDS
GATAGCAACTATTGTAAAAT+TGG 0.213801 4_4:-3644422 None:intergenic
CTGAACCCTTCACATTTCTT+TGG 0.240921 4_4:-3642088 None:intergenic
AATTCAACGGTTATGCTTAC+TGG 0.242093 4_4:+3641116 Msa0664610:CDS
CTTGAAACATTAACCTTGTT+TGG 0.254573 4_4:-3644238 None:intergenic
GAAATAGTGCAGTTGAATTA+TGG 0.270889 4_4:+3640985 Msa0664610:exon
AACGGTTATGCTTACTGGTT+TGG 0.341414 4_4:+3641121 Msa0664610:CDS
GTTAATCGTCTTGCTAGAAT+TGG 0.379939 4_4:+3642289 Msa0664610:CDS
TATGCTTACTGGTTTGGTCT+TGG 0.389422 4_4:+3641127 Msa0664610:CDS
CTGGAAATTCTATACCTAGT+TGG 0.392597 4_4:+3642266 Msa0664610:CDS
TCACCGGCTTCTTCTAGCTC+TGG 0.400392 4_4:+3644283 Msa0664610:CDS
TGGAAATTCTATACCTAGTT+GGG 0.400569 4_4:+3642267 Msa0664610:CDS
AACCAAGAAAGGCAACATAT+TGG 0.410635 4_4:+3644449 Msa0664610:three_prime_UTR
TTTAGAAAGACAATAATGAT+TGG 0.412372 4_4:+3642112 Msa0664610:CDS
TCACGAAACGGTGTCGTATC+GGG 0.415311 4_4:+3641055 Msa0664610:CDS
CATGGTGTTGAATATGCATT+TGG 0.429052 4_4:+3642025 Msa0664610:CDS
GTTTATCATTCTGGTGTTCA+AGG 0.430967 4_4:+3641151 Msa0664610:CDS
TATTAATTTGTTCAAGAGAT+GGG 0.448603 4_4:+3644579 Msa0664610:three_prime_UTR
TTCACGAAACGGTGTCGTAT+CGG 0.456858 4_4:+3641054 Msa0664610:CDS
TGGTTCTCCTTCAAATATAC+CGG 0.461685 4_4:-3642068 None:intergenic
TGTCGTATCGGGATCGGTGC+CGG 0.473134 4_4:+3641066 Msa0664610:CDS
TGCTATCAACAAACCAAGAA+AGG 0.486381 4_4:+3644438 Msa0664610:three_prime_UTR
CTTCTAGCTCTGGATTAAGA+AGG 0.486970 4_4:+3644293 Msa0664610:CDS
GCACATGAGTATGCATCAAC+CGG 0.506681 4_4:+3642049 Msa0664610:CDS
TTCTAGCTCTGGATTAAGAA+GGG 0.509805 4_4:+3644294 Msa0664610:CDS
GATGCTTGTGTTAAGCTCAC+TGG 0.515807 4_4:+3642247 Msa0664610:CDS
GCATCAACCGGTATATTTGA+AGG 0.517962 4_4:+3642061 Msa0664610:CDS
TTATTAATTTGTTCAAGAGA+TGG 0.520404 4_4:+3644578 Msa0664610:three_prime_UTR
TTTGTTGAGTTCAATGTCAC+GGG 0.532965 4_4:-3644136 None:intergenic
ACCTTCGAGAGCATCTTCAC+CGG 0.544046 4_4:+3644267 Msa0664610:CDS
TGTGGAAAAGTTTCACGAAA+CGG 0.553507 4_4:+3641043 Msa0664610:CDS
ATTAATTTGTTCAAGAGATG+GGG 0.555388 4_4:+3644580 Msa0664610:three_prime_UTR
CCGGCAAAACCCGCCGCCGG+TGG 0.563524 4_4:+3640791 Msa0664610:five_prime_UTR
GGAGAACCAAAGAAATGTGA+AGG 0.567060 4_4:+3642082 Msa0664610:CDS
GCAATAATCAAAGGTGAAGG+TGG 0.569859 4_4:-3644340 None:intergenic
AAACGGTGTCGTATCGGGAT+CGG 0.582657 4_4:+3641060 Msa0664610:CDS
ACACCGGCAAAACCCGCCGC+CGG 0.585578 4_4:+3640788 Msa0664610:five_prime_UTR
GATTAAGAAGGGGAAGGAGT+AGG 0.587384 4_4:+3644305 Msa0664610:CDS
TCTAGCTCTGGATTAAGAAG+GGG 0.590101 4_4:+3644295 Msa0664610:CDS
TGGTGAAGTGAGAAGTGTGA+TGG 0.594058 4_4:+3642153 Msa0664610:CDS
GCCGGTGAAGATGCTCTCGA+AGG 0.607232 4_4:-3644268 None:intergenic
CGTCGGAGAATGTTACTATG+TGG 0.607728 4_4:+3641025 Msa0664610:CDS
TAAACATCAAGATGAAGGGG+AGG 0.607838 4_4:-3644362 None:intergenic
ATAATCAAAGGTGAAGGTGG+AGG 0.615233 4_4:-3644337 None:intergenic
AATCCAGAGCTAGAAGAAGC+CGG 0.616375 4_4:-3644286 None:intergenic
ATTAACAAGTGAACCAAACA+AGG 0.618419 4_4:+3644225 Msa0664610:CDS
GAACTTGCTGCTGAGTACAA+AGG 0.625237 4_4:+3642178 Msa0664610:CDS
GAAGGGGAGGCAATAATCAA+AGG 0.625643 4_4:-3644349 None:intergenic
CATAACACAAAACTTTACAC+CGG 0.627597 4_4:+3640772 Msa0664610:five_prime_UTR
GCAAGACGATTAACCCAACT+AGG 0.629110 4_4:-3642280 None:intergenic
AAGAGAAGAAAACACATCGT+CGG 0.636912 4_4:+3641008 Msa0664610:CDS
TACGATCTGACACAATTCAA+CGG 0.645029 4_4:+3641103 Msa0664610:CDS
GAGAACCAAAGAAATGTGAA+GGG 0.652920 4_4:+3642083 Msa0664610:CDS
GAGGCAATAATCAAAGGTGA+AGG 0.662473 4_4:-3644343 None:intergenic
TCGTACACGTTAAGATACAC+CGG 0.689501 4_4:-3641085 None:intergenic
GCTCTGGATTAAGAAGGGGA+AGG 0.690627 4_4:+3644299 Msa0664610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAAATATAAGTAAAAAT+TGG - chr4_4:3641543-3641562 None:intergenic 10.0%
!!! CATTTCTATTTTTATTTTTT+TGG + chr4_4:3641910-3641929 Msa0664610:intron 10.0%
!!! TATTTTTATTTTTTTGGTTT+GGG + chr4_4:3641916-3641935 Msa0664610:intron 10.0%
!! AATAAATTGAAATAATTAGT+TGG + chr4_4:3642413-3642432 Msa0664610:intron 10.0%
!! ATAAATTGAAATAATTAGTT+GGG + chr4_4:3642414-3642433 Msa0664610:intron 10.0%
!!! TTTACATTTAATTTAAAGAA+AGG + chr4_4:3643555-3643574 Msa0664610:intron 10.0%
!!! TATTAATTTTAAAATGCATA+AGG + chr4_4:3643592-3643611 Msa0664610:intron 10.0%
!! TTTATATGCATTTAAATATA+TGG + chr4_4:3643696-3643715 Msa0664610:intron 10.0%
!! AAAAAATGTATAATAAAAGT+AGG + chr4_4:3643977-3643996 Msa0664610:intron 10.0%
!! TTTCCAATAAAAAAAATGAT+GGG - chr4_4:3640851-3640870 None:intergenic 15.0%
!! ATTTCCAATAAAAAAAATGA+TGG - chr4_4:3640852-3640871 None:intergenic 15.0%
!! ATTTCCAATAAAAAAATGAT+GGG - chr4_4:3640884-3640903 None:intergenic 15.0%
!! AATTTCCAATAAAAAAATGA+TGG - chr4_4:3640885-3640904 None:intergenic 15.0%
!! AAAATTTGAATCAAATACAT+CGG + chr4_4:3641506-3641525 Msa0664610:intron 15.0%
!!! TTTGTTAAAGACTTTTTTTT+AGG + chr4_4:3641868-3641887 Msa0664610:intron 15.0%
!!! TTGTTAAAGACTTTTTTTTA+GGG + chr4_4:3641869-3641888 Msa0664610:intron 15.0%
!!! CTATTTTTATTTTTTTGGTT+TGG + chr4_4:3641915-3641934 Msa0664610:intron 15.0%
!!! ATTTCAATTTATTTGTTTCT+TGG - chr4_4:3642406-3642425 None:intergenic 15.0%
!!! ATTATGTTTTAATTGATACA+CGG - chr4_4:3642522-3642541 None:intergenic 15.0%
!!! AGTGGTTTTTTTTTTTTTTA+AGG + chr4_4:3642691-3642710 Msa0664610:intron 15.0%
!!! TCAAAAAATGTATTTTTTCT+TGG + chr4_4:3643065-3643084 Msa0664610:intron 15.0%
!!! CAAAAAATGTATTTTTTCTT+GGG + chr4_4:3643066-3643085 Msa0664610:intron 15.0%
!!! AAAAGTATTTTACAAGTATT+AGG - chr4_4:3643492-3643511 None:intergenic 15.0%
!! TCTTTGTAACAAAAAAAAAT+AGG + chr4_4:3643947-3643966 Msa0664610:intron 15.0%
!!! TTTGAATGTGAAGTTTTTTT+TGG - chr4_4:3640820-3640839 None:intergenic 20.0%
!! TTCGGAAAAATTAAGAATTA+AGG - chr4_4:3641206-3641225 None:intergenic 20.0%
!! AGACTAGAATAATATTAATC+TGG - chr4_4:3641566-3641585 None:intergenic 20.0%
!! TTATTAGTACTTAGATTAGT+AGG + chr4_4:3641692-3641711 Msa0664610:intron 20.0%
!! TATTAGTACTTAGATTAGTA+GGG + chr4_4:3641693-3641712 Msa0664610:intron 20.0%
!!! CAAATTAGTCATTCTTTTTT+TGG - chr4_4:3641806-3641825 None:intergenic 20.0%
!!! GATTGATATTTGAAATTTTG+AGG + chr4_4:3641825-3641844 Msa0664610:intron 20.0%
!!! TATTTTGTTTTTTCAGTTCA+TGG + chr4_4:3642007-3642026 Msa0664610:intron 20.0%
!! TTTAGAAAGACAATAATGAT+TGG + chr4_4:3642112-3642131 Msa0664610:CDS 20.0%
!!! ACAATCTTTTGATAAGTTTT+AGG + chr4_4:3642735-3642754 Msa0664610:intron 20.0%
!! AATATCTACGAAACAAAAAT+TGG + chr4_4:3642879-3642898 Msa0664610:intron 20.0%
!!! ATTTACATTTGCTATAAATG+TGG + chr4_4:3642949-3642968 Msa0664610:intron 20.0%
!!! GATCAAGTCAAATTTTTTAT+TGG + chr4_4:3643016-3643035 Msa0664610:intron 20.0%
!! TAAAAATTACTAATCTACGT+TGG + chr4_4:3643125-3643144 Msa0664610:intron 20.0%
!! TAAAATAATACAACCAACAA+TGG - chr4_4:3643245-3643264 None:intergenic 20.0%
!! AAAATGTGAATAAATGATGT+GGG + chr4_4:3643404-3643423 Msa0664610:intron 20.0%
!!! TAATTTTAAAATGCATAAGG+TGG + chr4_4:3643595-3643614 Msa0664610:intron 20.0%
!!! ATTTTAAACATCAAGATGAA+GGG - chr4_4:3644369-3644388 None:intergenic 20.0%
!!! TTATTAATTTGTTCAAGAGA+TGG + chr4_4:3644578-3644597 Msa0664610:three_prime_UTR 20.0%
!!! TATTAATTTGTTCAAGAGAT+GGG + chr4_4:3644579-3644598 Msa0664610:three_prime_UTR 20.0%
!!! ATGCCCATCATTTTTTTTAT+TGG + chr4_4:3640845-3640864 Msa0664610:five_prime_UTR 25.0%
!!! TATGCCCATCATTTTTTTAT+TGG + chr4_4:3640877-3640896 Msa0664610:five_prime_UTR 25.0%
! TTTACTCAACGTTGAATTTA+CGG + chr4_4:3641311-3641330 Msa0664610:intron 25.0%
!! AATCAAATACATCGGATTTT+TGG + chr4_4:3641514-3641533 Msa0664610:intron 25.0%
! ATTTCGATTTCTAAACGAAA+TGG - chr4_4:3641720-3641739 None:intergenic 25.0%
! AATGATTGGAAAAACAGATA+TGG + chr4_4:3642126-3642145 Msa0664610:CDS 25.0%
!! GGAACATGTATTTTGTAATT+TGG - chr4_4:3642501-3642520 None:intergenic 25.0%
! TCAAAGATATTGAAAGTCAA+TGG - chr4_4:3642582-3642601 None:intergenic 25.0%
!! ATTGTTGACTAAAGCAAATT+CGG + chr4_4:3642618-3642637 Msa0664610:intron 25.0%
!! AGTATTGAATTCATTCATAG+TGG + chr4_4:3642673-3642692 Msa0664610:intron 25.0%
!!! ATAAGTTTTAGGCTGAATTT+AGG + chr4_4:3642746-3642765 Msa0664610:intron 25.0%
!!! TAAGTTTTAGGCTGAATTTA+GGG + chr4_4:3642747-3642766 Msa0664610:intron 25.0%
!!! GAGATTATAGTTGATAATGA+TGG + chr4_4:3642981-3643000 Msa0664610:intron 25.0%
! GAAAATGTGAATAAATGATG+TGG + chr4_4:3643403-3643422 Msa0664610:intron 25.0%
! TATACGCAATTAAATGACAT+TGG + chr4_4:3643459-3643478 Msa0664610:intron 25.0%
! TTCCCATGTATAAAAAAATG+TGG + chr4_4:3643794-3643813 Msa0664610:intron 25.0%
! TCCCATGTATAAAAAAATGT+GGG + chr4_4:3643795-3643814 Msa0664610:intron 25.0%
!!! TCCCACATTTTTTTATACAT+GGG - chr4_4:3643799-3643818 None:intergenic 25.0%
!!! TTCCCACATTTTTTTATACA+TGG - chr4_4:3643800-3643819 None:intergenic 25.0%
! TCTTGTACGTACAAATTATA+TGG + chr4_4:3643825-3643844 Msa0664610:intron 25.0%
! AAGCAATGTTTATCTCAATA+AGG - chr4_4:3644081-3644100 None:intergenic 25.0%
! AACATTGCTTATATTATTGC+AGG + chr4_4:3644091-3644110 Msa0664610:intron 25.0%
!! TTTTAAACATCAAGATGAAG+GGG - chr4_4:3644368-3644387 None:intergenic 25.0%
!! GATTTTAAACATCAAGATGA+AGG - chr4_4:3644370-3644389 None:intergenic 25.0%
! GATAGCAACTATTGTAAAAT+TGG - chr4_4:3644425-3644444 None:intergenic 25.0%
! AAGATATCAAAAAACAAGCA+AGG + chr4_4:3644535-3644554 Msa0664610:three_prime_UTR 25.0%
!!! ATTAATTTGTTCAAGAGATG+GGG + chr4_4:3644580-3644599 Msa0664610:three_prime_UTR 25.0%
CATAACACAAAACTTTACAC+CGG + chr4_4:3640772-3640791 Msa0664610:five_prime_UTR 30.0%
!!! AAGTTTTTTTTGGATTCCAC+CGG - chr4_4:3640810-3640829 None:intergenic 30.0%
!! GTTCTTCACTTTTCTCAATT+GGG - chr4_4:3640914-3640933 None:intergenic 30.0%
!! AGTTCTTCACTTTTCTCAAT+TGG - chr4_4:3640915-3640934 None:intergenic 30.0%
GAAATAGTGCAGTTGAATTA+TGG + chr4_4:3640985-3641004 Msa0664610:exon 30.0%
! GATTAGATCTAAAGCAAGTT+CGG - chr4_4:3641224-3641243 None:intergenic 30.0%
! ACTTGCTTTAGATCTAATCT+AGG + chr4_4:3641225-3641244 Msa0664610:intron 30.0%
TAGTCTCGAAAAACTTTGTT+GGG + chr4_4:3641580-3641599 Msa0664610:intron 30.0%
TGGAAATTCTATACCTAGTT+GGG + chr4_4:3642267-3642286 Msa0664610:CDS 30.0%
GAATAGTAGTAACAACTAGT+AGG - chr4_4:3642328-3642347 None:intergenic 30.0%
!!! TCTTGCTTTTTTTCCTGTTT+TGG + chr4_4:3642357-3642376 Msa0664610:intron 30.0%
ACATGATCAATATCCAAAAC+AGG - chr4_4:3642373-3642392 None:intergenic 30.0%
!! TGTTTTGGATATTGATCATG+TGG + chr4_4:3642372-3642391 Msa0664610:intron 30.0%
AATAATTAGTTGGGTGAAAG+TGG + chr4_4:3642423-3642442 Msa0664610:intron 30.0%
ATAATTAGTTGGGTGAAAGT+GGG + chr4_4:3642424-3642443 Msa0664610:intron 30.0%
! TTGTTGACTAAAGCAAATTC+GGG + chr4_4:3642619-3642638 Msa0664610:intron 30.0%
TAATGAGTTTCTGTTTGACT+AGG + chr4_4:3642650-3642669 Msa0664610:intron 30.0%
CTTGATCAAAATCTTCACAA+CGG - chr4_4:3642921-3642940 None:intergenic 30.0%
TAATACAACCAACAATGGAT+TGG - chr4_4:3643240-3643259 None:intergenic 30.0%
ACATATCAACTGCTAAGAAA+TGG - chr4_4:3643906-3643925 None:intergenic 30.0%
! GAACTTTTATGCTAAACTTC+CGG + chr4_4:3644038-3644057 Msa0664610:intron 30.0%
GGTGATAGATATCTTAATTC+CGG - chr4_4:3644060-3644079 None:intergenic 30.0%
! TTTTGTTGAGTTCAATGTCA+CGG - chr4_4:3644140-3644159 None:intergenic 30.0%
ATTAACAAGTGAACCAAACA+AGG + chr4_4:3644225-3644244 Msa0664610:CDS 30.0%
CTTGAAACATTAACCTTGTT+TGG - chr4_4:3644241-3644260 None:intergenic 30.0%
AAAAAACAAGCAAGGAATCA+TGG + chr4_4:3644543-3644562 Msa0664610:three_prime_UTR 30.0%
AAGAGAAGAAAACACATCGT+CGG + chr4_4:3641008-3641027 Msa0664610:CDS 35.0%
TGTGGAAAAGTTTCACGAAA+CGG + chr4_4:3641043-3641062 Msa0664610:CDS 35.0%
TACGATCTGACACAATTCAA+CGG + chr4_4:3641103-3641122 Msa0664610:CDS 35.0%
AATTCAACGGTTATGCTTAC+TGG + chr4_4:3641116-3641135 Msa0664610:CDS 35.0%
GTTTATCATTCTGGTGTTCA+AGG + chr4_4:3641151-3641170 Msa0664610:CDS 35.0%
! GATCTAATCTAGGATCTATG+TGG + chr4_4:3641235-3641254 Msa0664610:intron 35.0%
! TCTCTCTCTTTCTAAAAGTG+TGG + chr4_4:3641277-3641296 Msa0664610:intron 35.0%
CTAGTCTCGAAAAACTTTGT+TGG + chr4_4:3641579-3641598 Msa0664610:intron 35.0%
CTTTGTTGGGAATAACCAAA+TGG + chr4_4:3641593-3641612 Msa0664610:intron 35.0%
AGCTAGAATTCATCACCATT+TGG - chr4_4:3641611-3641630 None:intergenic 35.0%
! TGGTGATGAATTCTAGCTAA+AGG + chr4_4:3641613-3641632 Msa0664610:intron 35.0%
TCTAGCTAAAGGTGAATCAT+TGG + chr4_4:3641624-3641643 Msa0664610:intron 35.0%
! CATGGTGTTGAATATGCATT+TGG + chr4_4:3642025-3642044 Msa0664610:CDS 35.0%
TGGTTCTCCTTCAAATATAC+CGG - chr4_4:3642071-3642090 None:intergenic 35.0%
GAGAACCAAAGAAATGTGAA+GGG + chr4_4:3642083-3642102 Msa0664610:CDS 35.0%
GGAAAAACAGATATGGAACT+TGG + chr4_4:3642133-3642152 Msa0664610:CDS 35.0%
CACAAGCATCATTGCAAAAA+TGG - chr4_4:3642237-3642256 None:intergenic 35.0%
CTGGAAATTCTATACCTAGT+TGG + chr4_4:3642266-3642285 Msa0664610:CDS 35.0%
GTTAATCGTCTTGCTAGAAT+TGG + chr4_4:3642289-3642308 Msa0664610:CDS 35.0%
AATCTCAACACAACAACCAT+GGG - chr4_4:3642471-3642490 None:intergenic 35.0%
AAATCTCAACACAACAACCA+TGG - chr4_4:3642472-3642491 None:intergenic 35.0%
GTTGTTGTGTTGAGATTTGA+CGG + chr4_4:3642474-3642493 Msa0664610:intron 35.0%
TCTTTGATTGCGTAAATGTG+TGG + chr4_4:3642595-3642614 Msa0664610:intron 35.0%
! GGGATATAAGAACCTTTTCT+CGG + chr4_4:3642767-3642786 Msa0664610:intron 35.0%
! TAAGAACCTTTTCTCGGAAT+AGG + chr4_4:3642773-3642792 Msa0664610:intron 35.0%
AACTTTCCTATTCCGAGAAA+AGG - chr4_4:3642782-3642801 None:intergenic 35.0%
AATCCTTAAACACGTGATCA+GGG - chr4_4:3643196-3643215 None:intergenic 35.0%
TAATCCTTAAACACGTGATC+AGG - chr4_4:3643197-3643216 None:intergenic 35.0%
!! CAACAATGGATTGGTCTAAA+TGG - chr4_4:3643231-3643250 None:intergenic 35.0%
CATTTAGACCAATCCATTGT+TGG + chr4_4:3643229-3643248 Msa0664610:intron 35.0%
ATCAAAATCCTCAAGACCAT+TGG + chr4_4:3643330-3643349 Msa0664610:intron 35.0%
ATTAGGCAAATAGTCTCCAA+TGG - chr4_4:3643349-3643368 None:intergenic 35.0%
CAAATTCAACGTGTAGCATT+AGG - chr4_4:3643366-3643385 None:intergenic 35.0%
! TGCTACACGTTGAATTTGTT+AGG + chr4_4:3643368-3643387 Msa0664610:intron 35.0%
!! GGGAGAGTTACTTTAGAATT+AGG + chr4_4:3643424-3643443 Msa0664610:intron 35.0%
! TTCTTAGCAGTTGATATGTG+TGG + chr4_4:3643907-3643926 Msa0664610:intron 35.0%
TTTGTTGAGTTCAATGTCAC+GGG - chr4_4:3644139-3644158 None:intergenic 35.0%
!! TTCTAGCTCTGGATTAAGAA+GGG + chr4_4:3644294-3644313 Msa0664610:CDS 35.0%
TGCTATCAACAAACCAAGAA+AGG + chr4_4:3644438-3644457 Msa0664610:three_prime_UTR 35.0%
AACCAAGAAAGGCAACATAT+TGG + chr4_4:3644449-3644468 Msa0664610:three_prime_UTR 35.0%
ATCCAATATGTTGCCTTTCT+TGG - chr4_4:3644454-3644473 None:intergenic 35.0%
!!! TTTTTTTTGGATTCCACCGG+CGG - chr4_4:3640807-3640826 None:intergenic 40.0%
TCGTACACGTTAAGATACAC+CGG - chr4_4:3641088-3641107 None:intergenic 40.0%
! AACGGTTATGCTTACTGGTT+TGG + chr4_4:3641121-3641140 Msa0664610:CDS 40.0%
!! TATGCTTACTGGTTTGGTCT+TGG + chr4_4:3641127-3641146 Msa0664610:CDS 40.0%
!! GGTCTTGGTGTTTATCATTC+TGG + chr4_4:3641142-3641161 Msa0664610:CDS 40.0%
GCATCAACCGGTATATTTGA+AGG + chr4_4:3642061-3642080 Msa0664610:CDS 40.0%
GGAGAACCAAAGAAATGTGA+AGG + chr4_4:3642082-3642101 Msa0664610:CDS 40.0%
CTGAACCCTTCACATTTCTT+TGG - chr4_4:3642091-3642110 None:intergenic 40.0%
ATCCTTAAACACGTGATCAG+GGG - chr4_4:3643195-3643214 None:intergenic 40.0%
! AATAGTCTCCAATGGTCTTG+AGG - chr4_4:3643341-3643360 None:intergenic 40.0%
!! CTTCTAGCTCTGGATTAAGA+AGG + chr4_4:3644293-3644312 Msa0664610:CDS 40.0%
!! TCTAGCTCTGGATTAAGAAG+GGG + chr4_4:3644295-3644314 Msa0664610:CDS 40.0%
ATAATCAAAGGTGAAGGTGG+AGG - chr4_4:3644340-3644359 None:intergenic 40.0%
GCAATAATCAAAGGTGAAGG+TGG - chr4_4:3644343-3644362 None:intergenic 40.0%
GAGGCAATAATCAAAGGTGA+AGG - chr4_4:3644346-3644365 None:intergenic 40.0%
TAAACATCAAGATGAAGGGG+AGG - chr4_4:3644365-3644384 None:intergenic 40.0%
CGTCGGAGAATGTTACTATG+TGG + chr4_4:3641025-3641044 Msa0664610:CDS 45.0%
!! TTCACGAAACGGTGTCGTAT+CGG + chr4_4:3641054-3641073 Msa0664610:CDS 45.0%
GCACATGAGTATGCATCAAC+CGG + chr4_4:3642049-3642068 Msa0664610:CDS 45.0%
! TGGTGAAGTGAGAAGTGTGA+TGG + chr4_4:3642153-3642172 Msa0664610:CDS 45.0%
!! GAACTTGCTGCTGAGTACAA+AGG + chr4_4:3642178-3642197 Msa0664610:CDS 45.0%
GATGCTTGTGTTAAGCTCAC+TGG + chr4_4:3642247-3642266 Msa0664610:CDS 45.0%
GCAAGACGATTAACCCAACT+AGG - chr4_4:3642283-3642302 None:intergenic 45.0%
AAGTGGGAGCAATATGCAAG+CGG + chr4_4:3642440-3642459 Msa0664610:intron 45.0%
AAGTCAATGGATCGAGAACG+AGG - chr4_4:3642569-3642588 None:intergenic 45.0%
! AACACGTGATCAGGGGTTTT+TGG - chr4_4:3643188-3643207 None:intergenic 45.0%
AACCCCTGATCACGTGTTTA+AGG + chr4_4:3643190-3643209 Msa0664610:intron 45.0%
ACATTTGTCATGCCTGACGT+AGG + chr4_4:3643863-3643882 Msa0664610:intron 45.0%
TGCAAGTTCAAACCTACGTC+AGG - chr4_4:3643878-3643897 None:intergenic 45.0%
AATCCAGAGCTAGAAGAAGC+CGG - chr4_4:3644289-3644308 None:intergenic 45.0%
GATTAAGAAGGGGAAGGAGT+AGG + chr4_4:3644305-3644324 Msa0664610:CDS 45.0%
GAAGGGGAGGCAATAATCAA+AGG - chr4_4:3644352-3644371 None:intergenic 45.0%
!!! TCTATTTTTAATATAAAAAA+TGG + chr4_4:3641956-3641975 Msa0664610:intron 5.0%
!!! CTATTTTTAATATAAAAAAT+GGG + chr4_4:3641957-3641976 Msa0664610:intron 5.0%
!! TCACGAAACGGTGTCGTATC+GGG + chr4_4:3641055-3641074 Msa0664610:CDS 50.0%
!! AAACGGTGTCGTATCGGGAT+CGG + chr4_4:3641060-3641079 Msa0664610:CDS 50.0%
ACGTCTTGCTAACGAGTGTC+TGG + chr4_4:3641434-3641453 Msa0664610:intron 50.0%
CGTCTTGCTAACGAGTGTCT+GGG + chr4_4:3641435-3641454 Msa0664610:intron 50.0%
GTCTTGCTAACGAGTGTCTG+GGG + chr4_4:3641436-3641455 Msa0664610:intron 50.0%
AGTGGGAGCAATATGCAAGC+GGG + chr4_4:3642441-3642460 Msa0664610:intron 50.0%
!! ACCTTCGAGAGCATCTTCAC+CGG + chr4_4:3644267-3644286 Msa0664610:CDS 50.0%
! GCTCTGGATTAAGAAGGGGA+AGG + chr4_4:3644299-3644318 Msa0664610:CDS 50.0%
! TTTTTGGATTCCACCGGCGG+CGG - chr4_4:3640804-3640823 None:intergenic 55.0%
GTGGGAGCAATATGCAAGCG+GGG + chr4_4:3642442-3642461 Msa0664610:intron 55.0%
!! TCACCGGCTTCTTCTAGCTC+TGG + chr4_4:3644283-3644302 Msa0664610:CDS 55.0%
! TTTTGGATTCCACCGGCGGC+GGG - chr4_4:3640803-3640822 None:intergenic 60.0%
TGTCGTATCGGGATCGGTGC+CGG + chr4_4:3641066-3641085 Msa0664610:CDS 60.0%
GCCGGTGAAGATGCTCTCGA+AGG - chr4_4:3644271-3644290 None:intergenic 60.0%
TATGCAAGCGGGGTCGCCCA+TGG + chr4_4:3642452-3642471 Msa0664610:intron 65.0%
ACACCGGCAAAACCCGCCGC+CGG + chr4_4:3640788-3640807 Msa0664610:five_prime_UTR 70.0%
!! CCACCGGCGGCGGGTTTTGC+CGG - chr4_4:3640794-3640813 None:intergenic 75.0%
! CCGGCAAAACCCGCCGCCGG+TGG + chr4_4:3640791-3640810 Msa0664610:five_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 3640754 3644607 3640754 ID=Msa0664610;Name=Msa0664610
chr4_4 mRNA 3640754 3644607 3640754 ID=Msa0664610-mRNA-1;Parent=Msa0664610;Name=Msa0664610-mRNA-1;_AED=0.05;_eAED=0.05;_QI=250|1|1|1|1|1|3|229|240
chr4_4 exon 3640754 3641172 3640754 ID=Msa0664610-mRNA-1:exon:1200;Parent=Msa0664610-mRNA-1
chr4_4 exon 3642023 3642310 3642023 ID=Msa0664610-mRNA-1:exon:1201;Parent=Msa0664610-mRNA-1
chr4_4 exon 3644113 3644607 3644113 ID=Msa0664610-mRNA-1:exon:1202;Parent=Msa0664610-mRNA-1
chr4_4 five_prime_UTR 3640754 3641003 3640754 ID=Msa0664610-mRNA-1:five_prime_utr;Parent=Msa0664610-mRNA-1
chr4_4 CDS 3641004 3641172 3641004 ID=Msa0664610-mRNA-1:cds;Parent=Msa0664610-mRNA-1
chr4_4 CDS 3642023 3642310 3642023 ID=Msa0664610-mRNA-1:cds;Parent=Msa0664610-mRNA-1
chr4_4 CDS 3644113 3644378 3644113 ID=Msa0664610-mRNA-1:cds;Parent=Msa0664610-mRNA-1
chr4_4 three_prime_UTR 3644379 3644607 3644379 ID=Msa0664610-mRNA-1:three_prime_utr;Parent=Msa0664610-mRNA-1
Gene Sequence

>Msa0664610

ATGGAAGAGAAGAAAACACATCGTCGGAGAATGTTACTATGTGGAAAAGTTTCACGAAACGGTGTCGTATCGGGATCGGTGCCGGTGTATCTTAACGTGTACGATCTGACACAATTCAACGGTTATGCTTACTGGTTTGGTCTTGGTGTTTATCATTCTGGTGTTCAAGTTCATGGTGTTGAATATGCATTTGGTGCACATGAGTATGCATCAACCGGTATATTTGAAGGAGAACCAAAGAAATGTGAAGGGTTCAGATTTAGAAAGACAATAATGATTGGAAAAACAGATATGGAACTTGGTGAAGTGAGAAGTGTGATGGAAGAACTTGCTGCTGAGTACAAAGGAAATGCTTACAATCTCATTACAAAAAATTGTAACCATTTTTGCAATGATGCTTGTGTTAAGCTCACTGGAAATTCTATACCTAGTTGGGTTAATCGTCTTGCTAGAATTGGTTTTATGTGCAATTGTGTTCTTCCCGTGACATTGAACTCAACAAAAGTTAGACATCATCATCATAGAATAGAAGACAAACAATGTGACGATGATAAACAACTCAAACAAGCATTAACAAGTGAACCAAACAAGGTTAATGTTTCAAGTTCAACACCTTCGAGAGCATCTTCACCGGCTTCTTCTAGCTCTGGATTAAGAAGGGGAAGGAGTAGGACAAGACGTCCTCCACCTTCACCTTTGATTATTGCCTCCCCTTCATCTTGA
Protein sequence

>Msa0664610

MEEKKTHRRRMLLCGKVSRNGVVSGSVPVYLNVYDLTQFNGYAYWFGLGVYHSGVQVHGVEYAFGAHEYASTGIFEGEPKKCEGFRFRKTIMIGKTDMELGEVRSVMEELAAEYKGNAYNLITKNCNHFCNDACVKLTGNSIPSWVNRLARIGFMCNCVLPVTLNSTKVRHHHHRIEDKQCDDDKQLKQALTSEPNKVNVSSSTPSRASSPASSSSGLRRGRSRTRRPPPSPLIIASPSS*