AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594540 MtrunA17_Chr2g0286021 100.000 27 0 0 1 27 1 27 6.22e-13 63.2
Msa0594540 MtrunA17_Chr7g0218571 72.973 37 10 0 65 101 75 111 3.17e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 99 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCATAAGTTTAGGCTTT+AGG 0.170885 4_2:+22530480 Msa0594540:three_prime_UTR
AACCATAAGTTTAGGCTTTA+GGG 0.213110 4_2:+22530481 Msa0594540:three_prime_UTR
AAGCTATATTGTAGGGTATA+TGG 0.229586 4_2:+22530571 Msa0594540:three_prime_UTR
GACTTGTTTGGTGTAAATTA+AGG 0.241182 4_2:+22530820 Msa0594540:three_prime_UTR
TTCAAATAAGTTAATCTAAA+AGG 0.250881 4_2:+22530685 Msa0594540:three_prime_UTR
GCTTTAGTTCAAATTGCTTT+TGG 0.254423 4_2:+22529355 Msa0594540:three_prime_UTR
GGAGAAAATTGAGTACCTTT+TGG 0.281512 4_2:+22530841 Msa0594540:three_prime_UTR
GGAGTACTCCTTCAGGCTTA+TGG 0.286052 4_2:+22530917 Msa0594540:three_prime_UTR
ACTGCTATTATTATCATGTT+TGG 0.291212 4_2:+22529696 Msa0594540:three_prime_UTR
ATCCCTAAAGCCTAAACTTA+TGG 0.300096 4_2:-22530483 None:intergenic
GGAAGAAGGTTGGAGAGTTT+TGG 0.300993 4_2:+22529987 Msa0594540:three_prime_UTR
TCGCCCTCGTTCTTTCTTCA+AGG 0.310085 4_2:+22528185 Msa0594540:CDS
TACGCCACTATCTAAATTGC+AGG 0.347216 4_2:+22529604 Msa0594540:three_prime_UTR
CAGTTAGGACCGAGGCTTCT+TGG 0.352101 4_2:+22529128 Msa0594540:CDS
AGCGGCTGGTGTGTCGGTTT+TGG 0.352405 4_2:+22528992 Msa0594540:CDS
TTCTATTGCATGGCTAAGTA+TGG 0.352654 4_2:+22530054 Msa0594540:three_prime_UTR
AACATACACATAAAATTTGC+AGG 0.374225 4_2:-22529931 None:intergenic
AAAGCCATGTTTATGCAGTT+AGG 0.403852 4_2:+22529113 Msa0594540:CDS
CCTCGTTCTTTCTTCAAGGT+TGG 0.406404 4_2:+22528189 Msa0594540:CDS
TTACGAGAACTTATAGAAAC+AGG 0.407103 4_2:-22530629 None:intergenic
CAGCATTGGAGTACTCCTTC+AGG 0.411265 4_2:+22530910 Msa0594540:three_prime_UTR
AGTGGAGTTGTTAGTGGACT+TGG 0.412473 4_2:+22530869 Msa0594540:three_prime_UTR
GAAGTCCACAGTTGCCGATC+AGG 0.414976 4_2:+22530269 Msa0594540:three_prime_UTR
TAGGACCGAGGCTTCTTGGC+AGG 0.415660 4_2:+22529132 Msa0594540:CDS
TGATTCCACTTAGTGGGATA+AGG 0.416752 4_2:+22531078 Msa0594540:intron
GAGACAAGTGGAGTTGTTAG+TGG 0.419382 4_2:+22530863 Msa0594540:three_prime_UTR
TTGGCAGGGTTGACGATTCA+AGG 0.427680 4_2:+22529147 Msa0594540:CDS
GCAGCTGAAGAGGACTTGTT+TGG 0.442244 4_2:+22530808 Msa0594540:three_prime_UTR
TGCTGAGCTCTATAAATAAG+AGG 0.444835 4_2:-22529551 None:intergenic
CACTCTCCAAGCTATATTGT+AGG 0.446308 4_2:+22530563 Msa0594540:three_prime_UTR
TGCTGTTCTGTTATGACAGC+AGG 0.447721 4_2:-22528940 None:intergenic
GGAGTTACTGCTAGCAGCAT+TGG 0.459197 4_2:+22530896 Msa0594540:three_prime_UTR
TGATATGCTGTAGCAGTTGT+TGG 0.463306 4_2:+22530454 Msa0594540:three_prime_UTR
AGCTATATTGTAGGGTATAT+GGG 0.463513 4_2:+22530572 Msa0594540:three_prime_UTR
GAAAGAACGAGGGCGAAACT+TGG 0.463754 4_2:-22528178 None:intergenic
ATTCATAATGAAGTGAATAC+CGG 0.464662 4_2:+22529286 Msa0594540:CDS
TCTGTAGGCTGTGCAGCGGC+TGG 0.469312 4_2:+22528978 Msa0594540:CDS
TAGTATCTTTGCACCGGTAG+TGG 0.471506 4_2:+22529331 Msa0594540:exon
CAGTGGCTTCACTCATGCTC+AGG 0.473741 4_2:+22531007 Msa0594540:three_prime_UTR
CAACTCCACTTGTCTCCAAA+AGG 0.476312 4_2:-22530856 None:intergenic
GCTGTTGGTGCTGTTTCTGT+AGG 0.479485 4_2:+22528963 Msa0594540:CDS
TTGTAGCTGATTCCACTTAG+TGG 0.488437 4_2:+22531071 Msa0594540:intron
AAAGTGATAAATGAATAAGA+TGG 0.492984 4_2:+22530403 Msa0594540:three_prime_UTR
ATAACCTGCAATTTAGATAG+TGG 0.495770 4_2:-22529608 None:intergenic
TTGGTTACAACCATAAGTTT+AGG 0.503324 4_2:+22530473 Msa0594540:three_prime_UTR
TCCATGCGGAAATGGGAAGA+AGG 0.514677 4_2:+22529973 Msa0594540:three_prime_UTR
GCATTAGAATCTGACACTGC+AGG 0.515006 4_2:-22530161 None:intergenic
GCTCAGCAAATGAAAGTTAG+TGG 0.517412 4_2:+22529566 Msa0594540:three_prime_UTR
AAACTTTCGCGAAAGGCACT+CGG 0.518788 4_2:-22530219 None:intergenic
GAGAACTCAAATGAAAGATA+AGG 0.519927 4_2:-22529650 None:intergenic
AAGTGATAAATGAATAAGAT+GGG 0.522280 4_2:+22530404 Msa0594540:three_prime_UTR
TGCGGAAATGGGAAGAAGGT+TGG 0.524281 4_2:+22529977 Msa0594540:three_prime_UTR
GTTGTTAGTGGACTTGGGAA+AGG 0.528717 4_2:+22530875 Msa0594540:three_prime_UTR
TGCGTTTCGATCTTGTTCCC+CGG 0.532113 4_2:-22529305 None:intergenic
AAAAGCCTTATCCCACTAAG+TGG 0.532438 4_2:-22531083 None:intergenic
AAGACTATATTGTCAAAAGA+AGG 0.532861 4_2:-22529807 None:intergenic
AGGACCGAGGCTTCTTGGCA+GGG 0.533687 4_2:+22529133 Msa0594540:CDS
CTGTGCAGCGGCTGGTGTGT+CGG 0.534067 4_2:+22528986 Msa0594540:CDS
ATATACCCTACAATATAGCT+TGG 0.536716 4_2:-22530569 None:intergenic
ATAAACTAACTTCTATTGCA+TGG 0.542096 4_2:+22530044 Msa0594540:three_prime_UTR
TCTGGAGTAGGGCAGAGACT+TGG 0.544052 4_2:-22528133 None:intergenic
ACAACTGCTACAGCATATCA+TGG 0.544123 4_2:-22530451 None:intergenic
TTCATAATGAAGTGAATACC+GGG 0.553640 4_2:+22529287 Msa0594540:CDS
ATGAACCTGATCGGCAACTG+TGG 0.556047 4_2:-22530274 None:intergenic
TAATGACTATAAATGCACTC+TGG 0.559994 4_2:+22529874 Msa0594540:three_prime_UTR
TGTAGCTGATTCCACTTAGT+GGG 0.560518 4_2:+22531072 Msa0594540:intron
CAATTTAGATAGTGGCGTAA+AGG 0.562740 4_2:-22529600 None:intergenic
GAGAGTTAAACTTTCGCGAA+AGG 0.563012 4_2:-22530226 None:intergenic
ACTCTCCAAGCTATATTGTA+GGG 0.569220 4_2:+22530564 Msa0594540:three_prime_UTR
TGGCAGGGTTGACGATTCAA+GGG 0.571112 4_2:+22529148 Msa0594540:CDS
CAAACTGGCAGCACCTATCA+CGG 0.572986 4_2:-22529015 None:intergenic
TTGAACTAAAGCACCACTAC+CGG 0.574563 4_2:-22529344 None:intergenic
TCAACCCTGCCAAGAAGCCT+CGG 0.575981 4_2:-22529137 None:intergenic
ATCGAAATCCATAAGCCTGA+AGG 0.582144 4_2:-22530925 None:intergenic
CATAGGATAAGCAGCTGAAG+AGG 0.583093 4_2:+22530798 Msa0594540:three_prime_UTR
CAGGCGAAAACCGAAACCAC+AGG 0.584676 4_2:-22528921 None:intergenic
CGGTCCTAACTGCATAAACA+TGG 0.587699 4_2:-22529117 None:intergenic
ACCTTCTTCCCATTTCCGCA+TGG 0.587749 4_2:-22529974 None:intergenic
GTGGAGTTGTTAGTGGACTT+GGG 0.588312 4_2:+22530870 Msa0594540:three_prime_UTR
CTCAGCAAATGAAAGTTAGT+GGG 0.593841 4_2:+22529567 Msa0594540:three_prime_UTR
CATTAGAATCTGACACTGCA+GGG 0.594106 4_2:-22530160 None:intergenic
AAGTTAATCTAAAAGGAATG+AGG 0.596998 4_2:+22530692 Msa0594540:three_prime_UTR
AAAAGTGAAATGAACCTGAT+CGG 0.598666 4_2:-22530283 None:intergenic
CATGGCTAAGTATGGCAAAG+TGG 0.599899 4_2:+22530062 Msa0594540:three_prime_UTR
GCAACTTAGTATCTTTGCAC+CGG 0.601933 4_2:+22529325 Msa0594540:exon
CATAACAGAACAGCAGCTGT+TGG 0.608552 4_2:+22528948 Msa0594540:CDS
ATATGAAAGACTATACAACT+TGG 0.609818 4_2:-22529772 None:intergenic
AATGAAAGTTAGTGGGTGCG+TGG 0.628150 4_2:+22529574 Msa0594540:three_prime_UTR
GTTCAGCATTCAATCTACTG+TGG 0.633099 4_2:-22529900 None:intergenic
GATGAGATCAGTGTCTACAA+TGG 0.634391 4_2:+22530963 Msa0594540:three_prime_UTR
TGTTTATGCAGTTAGGACCG+AGG 0.648761 4_2:+22529120 Msa0594540:CDS
ATGGCTAAGTATGGCAAAGT+GGG 0.656929 4_2:+22530063 Msa0594540:three_prime_UTR
GAAAGTTAGTGGGTGCGTGG+AGG 0.661324 4_2:+22529577 Msa0594540:three_prime_UTR
CTAATTGTGATTCTAGACCT+CGG 0.667604 4_2:-22530091 None:intergenic
CCAACCTTGAAGAAAGAACG+AGG 0.679370 4_2:-22528189 None:intergenic
CAACCTTGAAGAAAGAACGA+GGG 0.685781 4_2:-22528188 None:intergenic
TGTTTCTGTAGGCTGTGCAG+CGG 0.701103 4_2:+22528974 Msa0594540:CDS
TGGCAAAGTGGGCTGCACCG+AGG 0.735778 4_2:+22530074 Msa0594540:three_prime_UTR
TCATAATGAAGTGAATACCG+GGG 0.737007 4_2:+22529288 Msa0594540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAATAAGATAATTGAAT+AGG - chr4_2:22528222-22528241 None:intergenic 15.0%
!! AAACATTGAATTAATAAATG+AGG - chr4_2:22528341-22528360 None:intergenic 15.0%
!!! AATTCAATGTTTTTAGTTTT+TGG + chr4_2:22528349-22528368 Msa0594540:intron 15.0%
!!! ATTCAATGTTTTTAGTTTTT+GGG + chr4_2:22528350-22528369 Msa0594540:intron 15.0%
!! AAAATGTATTTAATACAAGA+CGG - chr4_2:22528808-22528827 None:intergenic 15.0%
!! AAAACAAAAAATCAATTTGA+AGG - chr4_2:22529742-22529761 None:intergenic 15.0%
!!! GATTTTTTGTTTTTTTGTTT+TGG + chr4_2:22529749-22529768 Msa0594540:three_prime_UTR 15.0%
!!! AATTTTGTTTGTTTGTTTTA+GGG + chr4_2:22530508-22530527 Msa0594540:three_prime_UTR 15.0%
!! TTCAAATAAGTTAATCTAAA+AGG + chr4_2:22530685-22530704 Msa0594540:three_prime_UTR 15.0%
!! ATAAGATAATTGAATAGGAA+AGG - chr4_2:22528217-22528236 None:intergenic 20.0%
!! TTCAGCTTAATTAGTAATAT+TGG + chr4_2:22528562-22528581 Msa0594540:intron 20.0%
!! TCAGCTTAATTAGTAATATT+GGG + chr4_2:22528563-22528582 Msa0594540:intron 20.0%
!! AAGAAAGAAAGTGATAAAAT+CGG - chr4_2:22528712-22528731 None:intergenic 20.0%
!! ACCATTAATGTAAAAATTAG+GGG - chr4_2:22529235-22529254 None:intergenic 20.0%
!! CACCATTAATGTAAAAATTA+GGG - chr4_2:22529236-22529255 None:intergenic 20.0%
!!! TATTTTATTTGTGTGACTAA+AGG + chr4_2:22529403-22529422 Msa0594540:intron 20.0%
!! TTTAATATAAAACAAGCTAC+TGG - chr4_2:22529471-22529490 None:intergenic 20.0%
!! AAAGTGATAAATGAATAAGA+TGG + chr4_2:22530403-22530422 Msa0594540:three_prime_UTR 20.0%
!! AAGTGATAAATGAATAAGAT+GGG + chr4_2:22530404-22530423 Msa0594540:three_prime_UTR 20.0%
!!! CAATTTTGTTTGTTTGTTTT+AGG + chr4_2:22530507-22530526 Msa0594540:three_prime_UTR 20.0%
!!! TTTTGTTTTTCTGTTTTTCG+TGG + chr4_2:22528255-22528274 Msa0594540:intron 25.0%
!!! TTTGTTGTTGTTAGAATTGT+AGG + chr4_2:22528286-22528305 Msa0594540:intron 25.0%
!!! ACATTTTCTGTTTTAGTTGT+TGG + chr4_2:22528821-22528840 Msa0594540:intron 25.0%
!! ACCCCTAATTTTTACATTAA+TGG + chr4_2:22529231-22529250 Msa0594540:intron 25.0%
! GCACCATTAATGTAAAAATT+AGG - chr4_2:22529237-22529256 None:intergenic 25.0%
! ATTCATAATGAAGTGAATAC+CGG + chr4_2:22529286-22529305 Msa0594540:CDS 25.0%
! ACTGCTATTATTATCATGTT+TGG + chr4_2:22529696-22529715 Msa0594540:three_prime_UTR 25.0%
! ATATGAAAGACTATACAACT+TGG - chr4_2:22529775-22529794 None:intergenic 25.0%
! AAGACTATATTGTCAAAAGA+AGG - chr4_2:22529810-22529829 None:intergenic 25.0%
! AACATACACATAAAATTTGC+AGG - chr4_2:22529934-22529953 None:intergenic 25.0%
! ATAAACTAACTTCTATTGCA+TGG + chr4_2:22530044-22530063 Msa0594540:three_prime_UTR 25.0%
!! CAACTATTGTTGGAATTTTA+TGG + chr4_2:22530309-22530328 Msa0594540:three_prime_UTR 25.0%
! AAGTTAATCTAAAAGGAATG+AGG + chr4_2:22530692-22530711 Msa0594540:three_prime_UTR 25.0%
!! CTTATGGATTTCGATTTTAT+AGG + chr4_2:22530933-22530952 Msa0594540:three_prime_UTR 25.0%
!! TTTCGATTTTATAGGTCATT+TGG + chr4_2:22530941-22530960 Msa0594540:three_prime_UTR 25.0%
AAATATTCCAGAACATAGCA+AGG - chr4_2:22528422-22528441 None:intergenic 30.0%
TTAGTCATGCATACAATTGA+GGG - chr4_2:22528497-22528516 None:intergenic 30.0%
! TGACATGTTGTATTAGTTTG+AGG + chr4_2:22528755-22528774 Msa0594540:intron 30.0%
! GAGGTGTGATTTCTTTTATT+TGG + chr4_2:22528774-22528793 Msa0594540:intron 30.0%
TTCATAATGAAGTGAATACC+GGG + chr4_2:22529287-22529306 Msa0594540:CDS 30.0%
!! GCTTTAGTTCAAATTGCTTT+TGG + chr4_2:22529355-22529374 Msa0594540:three_prime_UTR 30.0%
CTGGTGAATTATCATATAAG+TGG - chr4_2:22529526-22529545 None:intergenic 30.0%
CTTATATGATAATTCACCAG+TGG + chr4_2:22529526-22529545 Msa0594540:intron 30.0%
ATAACCTGCAATTTAGATAG+TGG - chr4_2:22529611-22529630 None:intergenic 30.0%
GAGAACTCAAATGAAAGATA+AGG - chr4_2:22529653-22529672 None:intergenic 30.0%
TAATGACTATAAATGCACTC+TGG + chr4_2:22529874-22529893 Msa0594540:three_prime_UTR 30.0%
!!! GTGCTAATTTTTTTTCCATG+CGG + chr4_2:22529959-22529978 Msa0594540:three_prime_UTR 30.0%
!!! ATTTTTTTTCCATGCGGAAA+TGG + chr4_2:22529965-22529984 Msa0594540:three_prime_UTR 30.0%
!!! TTTTTTTTCCATGCGGAAAT+GGG + chr4_2:22529966-22529985 Msa0594540:three_prime_UTR 30.0%
AAAAGTGAAATGAACCTGAT+CGG - chr4_2:22530286-22530305 None:intergenic 30.0%
! CACTTTTATGCAACTATTGT+TGG + chr4_2:22530299-22530318 Msa0594540:three_prime_UTR 30.0%
TTGGTTACAACCATAAGTTT+AGG + chr4_2:22530473-22530492 Msa0594540:three_prime_UTR 30.0%
AACCATAAGTTTAGGCTTTA+GGG + chr4_2:22530481-22530500 Msa0594540:three_prime_UTR 30.0%
ATATACCCTACAATATAGCT+TGG - chr4_2:22530572-22530591 None:intergenic 30.0%
AAGCTATATTGTAGGGTATA+TGG + chr4_2:22530571-22530590 Msa0594540:three_prime_UTR 30.0%
AGCTATATTGTAGGGTATAT+GGG + chr4_2:22530572-22530591 Msa0594540:three_prime_UTR 30.0%
TTACGAGAACTTATAGAAAC+AGG - chr4_2:22530632-22530651 None:intergenic 30.0%
!! TTGTTTTGTGCATTTTGCAT+AGG + chr4_2:22530781-22530800 Msa0594540:three_prime_UTR 30.0%
!! GACTTGTTTGGTGTAAATTA+AGG + chr4_2:22530820-22530839 Msa0594540:three_prime_UTR 30.0%
!!! TCAACATCTTTTCTGGAGTA+GGG - chr4_2:22528147-22528166 None:intergenic 35.0%
! ATCGTAGTCAACATCTTTTC+TGG - chr4_2:22528154-22528173 None:intergenic 35.0%
GCTAAGAAATTGTGACAGTA+GGG - chr4_2:22528389-22528408 None:intergenic 35.0%
AGCTAAGAAATTGTGACAGT+AGG - chr4_2:22528390-22528409 None:intergenic 35.0%
! ATTAGTTCCTTGCTATGTTC+TGG + chr4_2:22528412-22528431 Msa0594540:intron 35.0%
GTTAGTCATGCATACAATTG+AGG - chr4_2:22528498-22528517 None:intergenic 35.0%
TGAGATGAGATGAGGTAATA+AGG + chr4_2:22528533-22528552 Msa0594540:intron 35.0%
GAGATGAGATGAGGTAATAA+GGG + chr4_2:22528534-22528553 Msa0594540:intron 35.0%
CAAATACTAATTTCTCGAGC+TGG - chr4_2:22528670-22528689 None:intergenic 35.0%
TTAGTTGTTGGATGACGTAA+CGG + chr4_2:22528833-22528852 Msa0594540:intron 35.0%
! TTCGCTATGGGTTTGAATTT+TGG + chr4_2:22529073-22529092 Msa0594540:intron 35.0%
AAAGCCATGTTTATGCAGTT+AGG + chr4_2:22529113-22529132 Msa0594540:CDS 35.0%
TCATAATGAAGTGAATACCG+GGG + chr4_2:22529288-22529307 Msa0594540:CDS 35.0%
CTATAAATAAGAGGATCCAC+TGG - chr4_2:22529545-22529564 None:intergenic 35.0%
TGCTGAGCTCTATAAATAAG+AGG - chr4_2:22529554-22529573 None:intergenic 35.0%
CTCAGCAAATGAAAGTTAGT+GGG + chr4_2:22529567-22529586 Msa0594540:three_prime_UTR 35.0%
! CAATTTAGATAGTGGCGTAA+AGG - chr4_2:22529603-22529622 None:intergenic 35.0%
AGCAGTCAAACAAAAATGCA+TGG - chr4_2:22529682-22529701 None:intergenic 35.0%
!!! TGGAGAGTTTTGGCTTTATT+TGG + chr4_2:22529997-22530016 Msa0594540:three_prime_UTR 35.0%
TTCTATTGCATGGCTAAGTA+TGG + chr4_2:22530054-22530073 Msa0594540:three_prime_UTR 35.0%
CTAATTGTGATTCTAGACCT+CGG - chr4_2:22530094-22530113 None:intergenic 35.0%
CAACCATAAGTTTAGGCTTT+AGG + chr4_2:22530480-22530499 Msa0594540:three_prime_UTR 35.0%
ATCCCTAAAGCCTAAACTTA+TGG - chr4_2:22530486-22530505 None:intergenic 35.0%
ACTCTCCAAGCTATATTGTA+GGG + chr4_2:22530564-22530583 Msa0594540:three_prime_UTR 35.0%
! GGAGAAAATTGAGTACCTTT+TGG + chr4_2:22530841-22530860 Msa0594540:three_prime_UTR 35.0%
!!! GTCAACATCTTTTCTGGAGT+AGG - chr4_2:22528148-22528167 None:intergenic 40.0%
CAACCTTGAAGAAAGAACGA+GGG - chr4_2:22528191-22528210 None:intergenic 40.0%
TGTTGTGATGAGATGAGATG+AGG + chr4_2:22528525-22528544 Msa0594540:intron 40.0%
GGGTTTAAATTCCGTGTCTT+AGG + chr4_2:22528583-22528602 Msa0594540:intron 40.0%
ACCGGCGTTAAAAGTCTTTA+GGG + chr4_2:22528611-22528630 Msa0594540:intron 40.0%
! ACCCTAAAGACTTTTAACGC+CGG - chr4_2:22528615-22528634 None:intergenic 40.0%
TTAGTATTTGTAGCTGCGTG+TGG + chr4_2:22528680-22528699 Msa0594540:intron 40.0%
TAGTATTTGTAGCTGCGTGT+GGG + chr4_2:22528681-22528700 Msa0594540:intron 40.0%
! CAACTTAACTAGTAGCCTTG+TGG + chr4_2:22528870-22528889 Msa0594540:intron 40.0%
GCAACTTAGTATCTTTGCAC+CGG + chr4_2:22529325-22529344 Msa0594540:exon 40.0%
! TTGAACTAAAGCACCACTAC+CGG - chr4_2:22529347-22529366 None:intergenic 40.0%
GCTCAGCAAATGAAAGTTAG+TGG + chr4_2:22529566-22529585 Msa0594540:three_prime_UTR 40.0%
TACGCCACTATCTAAATTGC+AGG + chr4_2:22529604-22529623 Msa0594540:three_prime_UTR 40.0%
GTTCAGCATTCAATCTACTG+TGG - chr4_2:22529903-22529922 None:intergenic 40.0%
ATGGCTAAGTATGGCAAAGT+GGG + chr4_2:22530063-22530082 Msa0594540:three_prime_UTR 40.0%
CATTAGAATCTGACACTGCA+GGG - chr4_2:22530163-22530182 None:intergenic 40.0%
GAGAGTTAAACTTTCGCGAA+AGG - chr4_2:22530229-22530248 None:intergenic 40.0%
ACAACTGCTACAGCATATCA+TGG - chr4_2:22530454-22530473 None:intergenic 40.0%
TGATATGCTGTAGCAGTTGT+TGG + chr4_2:22530454-22530473 Msa0594540:three_prime_UTR 40.0%
CACTCTCCAAGCTATATTGT+AGG + chr4_2:22530563-22530582 Msa0594540:three_prime_UTR 40.0%
ATCGAAATCCATAAGCCTGA+AGG - chr4_2:22530928-22530947 None:intergenic 40.0%
GATGAGATCAGTGTCTACAA+TGG + chr4_2:22530963-22530982 Msa0594540:three_prime_UTR 40.0%
! TTCTACACTTTTGCTCTCAG+TGG + chr4_2:22530990-22531009 Msa0594540:three_prime_UTR 40.0%
! TTGTAGCTGATTCCACTTAG+TGG + chr4_2:22531071-22531090 Msa0594540:intron 40.0%
! TGTAGCTGATTCCACTTAGT+GGG + chr4_2:22531072-22531091 Msa0594540:intron 40.0%
TGATTCCACTTAGTGGGATA+AGG + chr4_2:22531078-22531097 Msa0594540:intron 40.0%
! AAAAGCCTTATCCCACTAAG+TGG - chr4_2:22531086-22531105 None:intergenic 40.0%
CCAACCTTGAAGAAAGAACG+AGG - chr4_2:22528192-22528211 None:intergenic 45.0%
CCTCGTTCTTTCTTCAAGGT+TGG + chr4_2:22528189-22528208 Msa0594540:CDS 45.0%
CACCGGCGTTAAAAGTCTTT+AGG + chr4_2:22528610-22528629 Msa0594540:intron 45.0%
CTAATTTCTCGAGCTGGAAC+TGG - chr4_2:22528664-22528683 None:intergenic 45.0%
AGTATTTGTAGCTGCGTGTG+GGG + chr4_2:22528682-22528701 Msa0594540:intron 45.0%
TGCTGTTCTGTTATGACAGC+AGG - chr4_2:22528943-22528962 None:intergenic 45.0%
CATAACAGAACAGCAGCTGT+TGG + chr4_2:22528948-22528967 Msa0594540:CDS 45.0%
!!! CCAGTTTGGGGTTTTAGCTT+GGG + chr4_2:22529030-22529049 Msa0594540:intron 45.0%
!!! CAGTTTGGGGTTTTAGCTTG+GGG + chr4_2:22529031-22529050 Msa0594540:intron 45.0%
CGGTCCTAACTGCATAAACA+TGG - chr4_2:22529120-22529139 None:intergenic 45.0%
TGTTTATGCAGTTAGGACCG+AGG + chr4_2:22529120-22529139 Msa0594540:CDS 45.0%
!! ATTAATGGTGCTGCAGAGAC+TGG + chr4_2:22529246-22529265 Msa0594540:intron 45.0%
! TAGTATCTTTGCACCGGTAG+TGG + chr4_2:22529331-22529350 Msa0594540:exon 45.0%
! AATGAAAGTTAGTGGGTGCG+TGG + chr4_2:22529574-22529593 Msa0594540:three_prime_UTR 45.0%
! GGAAGAAGGTTGGAGAGTTT+TGG + chr4_2:22529987-22530006 Msa0594540:three_prime_UTR 45.0%
CATGGCTAAGTATGGCAAAG+TGG + chr4_2:22530062-22530081 Msa0594540:three_prime_UTR 45.0%
GCATTAGAATCTGACACTGC+AGG - chr4_2:22530164-22530183 None:intergenic 45.0%
!! AAACTTTCGCGAAAGGCACT+CGG - chr4_2:22530222-22530241 None:intergenic 45.0%
CATAGGATAAGCAGCTGAAG+AGG + chr4_2:22530798-22530817 Msa0594540:three_prime_UTR 45.0%
!! GAGTACCTTTTGGAGACAAG+TGG + chr4_2:22530851-22530870 Msa0594540:three_prime_UTR 45.0%
CAACTCCACTTGTCTCCAAA+AGG - chr4_2:22530859-22530878 None:intergenic 45.0%
GAGACAAGTGGAGTTGTTAG+TGG + chr4_2:22530863-22530882 Msa0594540:three_prime_UTR 45.0%
!! AGTGGAGTTGTTAGTGGACT+TGG + chr4_2:22530869-22530888 Msa0594540:three_prime_UTR 45.0%
!! GTGGAGTTGTTAGTGGACTT+GGG + chr4_2:22530870-22530889 Msa0594540:three_prime_UTR 45.0%
!! GTTGTTAGTGGACTTGGGAA+AGG + chr4_2:22530875-22530894 Msa0594540:three_prime_UTR 45.0%
!!! TTTTATTTTATATCAATTAT+CGG + chr4_2:22529207-22529226 Msa0594540:intron 5.0%
GAAAGAACGAGGGCGAAACT+TGG - chr4_2:22528181-22528200 None:intergenic 50.0%
TCGCCCTCGTTCTTTCTTCA+AGG + chr4_2:22528185-22528204 Msa0594540:CDS 50.0%
TCCGTGTCTTAGGAATGCAC+CGG + chr4_2:22528593-22528612 Msa0594540:intron 50.0%
! AGCCTTGTGGTTGTTGTTCG+TGG + chr4_2:22528883-22528902 Msa0594540:intron 50.0%
AGCCACGAACAACAACCACA+AGG - chr4_2:22528888-22528907 None:intergenic 50.0%
! TTGTGGTTGTTGTTCGTGGC+TGG + chr4_2:22528887-22528906 Msa0594540:intron 50.0%
!! GCTGTTGGTGCTGTTTCTGT+AGG + chr4_2:22528963-22528982 Msa0594540:CDS 50.0%
! TGTTTCTGTAGGCTGTGCAG+CGG + chr4_2:22528974-22528993 Msa0594540:CDS 50.0%
CAAACTGGCAGCACCTATCA+CGG - chr4_2:22529018-22529037 None:intergenic 50.0%
! GTGATAGGTGCTGCCAGTTT+GGG + chr4_2:22529017-22529036 Msa0594540:intron 50.0%
! TGATAGGTGCTGCCAGTTTG+GGG + chr4_2:22529018-22529037 Msa0594540:intron 50.0%
!!! GCCAGTTTGGGGTTTTAGCT+TGG + chr4_2:22529029-22529048 Msa0594540:intron 50.0%
CCCAAGCTAAAACCCCAAAC+TGG - chr4_2:22529033-22529052 None:intergenic 50.0%
!! TTTAGCTTGGGGCTGTTCAG+CGG + chr4_2:22529042-22529061 Msa0594540:intron 50.0%
TTGGCAGGGTTGACGATTCA+AGG + chr4_2:22529147-22529166 Msa0594540:CDS 50.0%
TGGCAGGGTTGACGATTCAA+GGG + chr4_2:22529148-22529167 Msa0594540:CDS 50.0%
TGCGTTTCGATCTTGTTCCC+CGG - chr4_2:22529308-22529327 None:intergenic 50.0%
TCCATGCGGAAATGGGAAGA+AGG + chr4_2:22529973-22529992 Msa0594540:three_prime_UTR 50.0%
ACCTTCTTCCCATTTCCGCA+TGG - chr4_2:22529977-22529996 None:intergenic 50.0%
TGCGGAAATGGGAAGAAGGT+TGG + chr4_2:22529977-22529996 Msa0594540:three_prime_UTR 50.0%
ATGAACCTGATCGGCAACTG+TGG - chr4_2:22530277-22530296 None:intergenic 50.0%
! GCAGCTGAAGAGGACTTGTT+TGG + chr4_2:22530808-22530827 Msa0594540:three_prime_UTR 50.0%
GGAGTTACTGCTAGCAGCAT+TGG + chr4_2:22530896-22530915 Msa0594540:three_prime_UTR 50.0%
! CAGCATTGGAGTACTCCTTC+AGG + chr4_2:22530910-22530929 Msa0594540:three_prime_UTR 50.0%
! GGAGTACTCCTTCAGGCTTA+TGG + chr4_2:22530917-22530936 Msa0594540:three_prime_UTR 50.0%
! TCTGGAGTAGGGCAGAGACT+TGG - chr4_2:22528136-22528155 None:intergenic 55.0%
GCCGGTGCATTCCTAAGACA+CGG - chr4_2:22528597-22528616 None:intergenic 55.0%
TTCTCGAGCTGGAACTGGAC+AGG - chr4_2:22528659-22528678 None:intergenic 55.0%
! TGTTGTTCGTGGCTGGCTGT+AGG + chr4_2:22528894-22528913 Msa0594540:intron 55.0%
! GTTGTTCGTGGCTGGCTGTA+GGG + chr4_2:22528895-22528914 Msa0594540:intron 55.0%
! TTGTTCGTGGCTGGCTGTAG+GGG + chr4_2:22528896-22528915 Msa0594540:intron 55.0%
TGTAGGGGGTCCTGTGGTTT+CGG + chr4_2:22528911-22528930 Msa0594540:intron 55.0%
CAGGCGAAAACCGAAACCAC+AGG - chr4_2:22528924-22528943 None:intergenic 55.0%
!!! GTGTCGGTTTTGGCCGTGAT+AGG + chr4_2:22529002-22529021 Msa0594540:CDS 55.0%
! CGTGATAGGTGCTGCCAGTT+TGG + chr4_2:22529016-22529035 Msa0594540:intron 55.0%
! TTAGCTTGGGGCTGTTCAGC+GGG + chr4_2:22529043-22529062 Msa0594540:intron 55.0%
! AGCGGGTAATGGCTTCGCTA+TGG + chr4_2:22529060-22529079 Msa0594540:intron 55.0%
! GCGGGTAATGGCTTCGCTAT+GGG + chr4_2:22529061-22529080 Msa0594540:intron 55.0%
CAGTTAGGACCGAGGCTTCT+TGG + chr4_2:22529128-22529147 Msa0594540:CDS 55.0%
TCAACCCTGCCAAGAAGCCT+CGG - chr4_2:22529140-22529159 None:intergenic 55.0%
! GAAAGTTAGTGGGTGCGTGG+AGG + chr4_2:22529577-22529596 Msa0594540:three_prime_UTR 55.0%
GAAGTCCACAGTTGCCGATC+AGG + chr4_2:22530269-22530288 Msa0594540:three_prime_UTR 55.0%
CAGTGGCTTCACTCATGCTC+AGG + chr4_2:22531007-22531026 Msa0594540:three_prime_UTR 55.0%
! TGTTCGTGGCTGGCTGTAGG+GGG + chr4_2:22528897-22528916 Msa0594540:intron 60.0%
!!! AGCGGCTGGTGTGTCGGTTT+TGG + chr4_2:22528992-22529011 Msa0594540:CDS 60.0%
TGGGGCTGTTCAGCGGGTAA+TGG + chr4_2:22529049-22529068 Msa0594540:intron 60.0%
TAGGACCGAGGCTTCTTGGC+AGG + chr4_2:22529132-22529151 Msa0594540:CDS 60.0%
AGGACCGAGGCTTCTTGGCA+GGG + chr4_2:22529133-22529152 Msa0594540:CDS 60.0%
TCTGTAGGCTGTGCAGCGGC+TGG + chr4_2:22528978-22528997 Msa0594540:CDS 65.0%
CTGTGCAGCGGCTGGTGTGT+CGG + chr4_2:22528986-22529005 Msa0594540:CDS 65.0%
!! TGGCAAAGTGGGCTGCACCG+AGG + chr4_2:22530074-22530093 Msa0594540:three_prime_UTR 65.0%
GCTGGCTGTAGGGGGTCCTG+TGG + chr4_2:22528905-22528924 Msa0594540:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 22528129 22531120 22528129 ID=Msa0594540;Name=Msa0594540
chr4_2 mRNA 22528129 22531120 22528129 ID=Msa0594540-mRNA-1;Parent=Msa0594540;Name=Msa0594540-mRNA-1;_AED=0.16;_eAED=0.19;_QI=0|0.6|0.5|1|0.6|0.5|6|1583|107
chr4_2 exon 22528129 22528210 22528129 ID=Msa0594540-mRNA-1:exon:6112;Parent=Msa0594540-mRNA-1
chr4_2 exon 22528916 22529023 22528916 ID=Msa0594540-mRNA-1:exon:6113;Parent=Msa0594540-mRNA-1
chr4_2 exon 22529108 22529169 22529108 ID=Msa0594540-mRNA-1:exon:6114;Parent=Msa0594540-mRNA-1
chr4_2 exon 22529262 22529376 22529262 ID=Msa0594540-mRNA-1:exon:6115;Parent=Msa0594540-mRNA-1
chr4_2 exon 22529546 22531044 22529546 ID=Msa0594540-mRNA-1:exon:6116;Parent=Msa0594540-mRNA-1
chr4_2 exon 22531080 22531120 22531080 ID=Msa0594540-mRNA-1:exon:6117;Parent=Msa0594540-mRNA-1
chr4_2 CDS 22528129 22528210 22528129 ID=Msa0594540-mRNA-1:cds;Parent=Msa0594540-mRNA-1
chr4_2 CDS 22528916 22529023 22528916 ID=Msa0594540-mRNA-1:cds;Parent=Msa0594540-mRNA-1
chr4_2 CDS 22529108 22529169 22529108 ID=Msa0594540-mRNA-1:cds;Parent=Msa0594540-mRNA-1
chr4_2 CDS 22529262 22529333 22529262 ID=Msa0594540-mRNA-1:cds;Parent=Msa0594540-mRNA-1
chr4_2 three_prime_UTR 22529334 22529376 22529334 ID=Msa0594540-mRNA-1:three_prime_utr;Parent=Msa0594540-mRNA-1
chr4_2 three_prime_UTR 22529546 22531044 22529546 ID=Msa0594540-mRNA-1:three_prime_utr;Parent=Msa0594540-mRNA-1
chr4_2 three_prime_UTR 22531080 22531120 22531080 ID=Msa0594540-mRNA-1:three_prime_utr;Parent=Msa0594540-mRNA-1
Gene Sequence

>Msa0594540

ATGTCCAAGTCTCTGCCCTACTCCAGAAAAGATGTTGACTACGATAACGCCAAGTTTCGCCCTCGTTCTTTCTTCAAGGTTGGGGGTCCTGTGGTTTCGGTTTTCGCCTGCTGTCATAACAGAACAGCAGCTGTTGGTGCTGTTTCTGTAGGCTGTGCAGCGGCTGGTGTGTCGGTTTTGGCCGTGATAGATTTCAAAGCCATGTTTATGCAGTTAGGACCGAGGCTTCTTGGCAGGGTTGACGATTCAAGGAGACTGGTTAGAGATTTTTATAAGATTCATAATGAAGTGAATACCGGGGAACAAGATCGAAACGCAACTTAG
Protein sequence

>Msa0594540

MSKSLPYSRKDVDYDNAKFRPRSFFKVGGPVVSVFACCHNRTAAVGAVSVGCAAAGVSVLAVIDFKAMFMQLGPRLLGRVDDSRRLVRDFYKIHNEVNTGEQDRNAT*