AlfalfaGEDB | Msa0599260

M. sativa cultivar zhongmu-4 / Msa0599260

KEGG: -
GO: -
PFAM: PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0599260	XP_013442530.1	95.678	671	24	3	1	670	1	667	0.0	1282

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0599260	sp\|Q54Q39\|PP1R7_DICDI	33.333	177	97	4	398	567	145	307	9.00e-13	73.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0599260	A0A072TSM4	95.678	671	24	3	1	670	1	667	0.0	1282

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0204780	Msa0599260	0.801497	8.755754e-49	-8.615850e-47
Msa0260790	Msa0599260	0.821023	5.032070e-53	-8.615850e-47
Msa0260800	Msa0599260	0.828652	7.979488e-55	-8.615850e-47
Msa0599260	Msa0678260	0.819383	1.195148e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0599260	MtrunA17_Chr4g0023131	95.678	671	24	3	1	670	1	667	0.0	1282
Msa0599260	MtrunA17_Chr3g0115891	53.600	250	107	2	328	569	358	606	4.69e-77	259
Msa0599260	MtrunA17_Chr5g0426251	50.000	254	111	4	328	570	337	585	1.24e-68	236

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0599260	AT4G03260.1	45.042	706	316	21	1	666	1	674	9.05e-157	468
Msa0599260	AT4G03260.4	45.042	706	316	21	1	666	1	674	9.05e-157	468
Msa0599260	AT4G03260.3	45.042	706	316	21	1	666	1	674	9.05e-157	468
Msa0599260	AT4G03260.2	45.042	706	316	21	1	666	1	674	9.05e-157	468
Msa0599260	AT1G78230.1	49.818	275	125	3	326	592	365	634	5.04e-74	252
Msa0599260	AT5G22320.1	32.174	230	132	7	369	591	69	281	2.05e-15	79.7
Msa0599260	AT5G22320.2	32.474	194	111	5	409	591	81	265	3.50e-11	66.2

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Find 200 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGTAAAAGAGAAAAG+AGG	0.490876	4_2:-38389265	None:intergenic
AAACAAGATTGGTGAAGTCG+AGG	0.607377	4_2:+38392238	Msa0599260:CDS
AAACAATATCTCCACTATAG+AGG	0.423035	4_2:+38391934	Msa0599260:CDS
AAACCGTTGAACTTGAATTC+AGG	0.370581	4_2:+38392710	Msa0599260:CDS
AAAGAGGAGAGACTTGATGA+TGG	0.499180	4_2:-38389249	None:intergenic
AAAGCAAATGGTTTGAAGGA+CGG	0.557095	4_2:+38392476	Msa0599260:CDS
AAATGGTTTGAAGGACGGTG+GGG	0.673778	4_2:+38392481	Msa0599260:CDS
AACAAGATTGGTGAAGTCGA+GGG	0.578646	4_2:+38392239	Msa0599260:CDS
AACAATATCTCCACTATAGA+GGG	0.516643	4_2:+38391935	Msa0599260:CDS
AACATGCATCTTCAATTTCA+AGG	0.340067	4_2:+38391173	Msa0599260:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACACAAACGGATAAATTG+CGG	-	chr4_2:38389867-38389886	None:intergenic	30.0%
CAACAATAGTAGCAAATTCA+CGG	+	chr4_2:38390273-38390292	Msa0599260:five_prime_UTR	30.0%
TGAACAGCATAGATTATTGT+TGG	+	chr4_2:38390323-38390342	Msa0599260:five_prime_UTR	30.0%
TAGACTTTGTATAACTTGGT+TGG	+	chr4_2:38390472-38390491	Msa0599260:intron	30.0%
GCTTAAAGTCAAAATAATCC+AGG	-	chr4_2:38390503-38390522	None:intergenic	30.0%
TAAATCTTCAACTGAAGCAA+TGG	+	chr4_2:38390547-38390566	Msa0599260:CDS	30.0%
TATGACTCACCAAATTCAAT+TGG	+	chr4_2:38390872-38390891	Msa0599260:CDS	30.0%
AAGATGCATGTTTAAAAGCA+TGG	-	chr4_2:38391165-38391184	None:intergenic	30.0%
AACATGCATCTTCAATTTCA+AGG	+	chr4_2:38391173-38391192	Msa0599260:CDS	30.0%
TGTGTCTTATGATCAAATAG+GGG	+	chr4_2:38391249-38391268	Msa0599260:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	38389077	38393078	38389077	ID=Msa0599260;Name=Msa0599260
chr4_2	mRNA	38389077	38393078	38389077	ID=Msa0599260-mRNA-1;Parent=Msa0599260;Name=Msa0599260-mRNA-1;_AED=0.02;_eAED=0.02;_QI=597\|1\|1\|1\|0.6\|0.33\|6\|274\|670
chr4_2	exon	38389077	38389292	38389077	ID=Msa0599260-mRNA-1:exon:8476;Parent=Msa0599260-mRNA-1
chr4_2	exon	38390012	38390443	38390012	ID=Msa0599260-mRNA-1:exon:8477;Parent=Msa0599260-mRNA-1
chr4_2	exon	38390539	38391763	38390539	ID=Msa0599260-mRNA-1:exon:8478;Parent=Msa0599260-mRNA-1
chr4_2	exon	38391879	38392025	38391879	ID=Msa0599260-mRNA-1:exon:8479;Parent=Msa0599260-mRNA-1
chr4_2	exon	38392195	38392849	38392195	ID=Msa0599260-mRNA-1:exon:8480;Parent=Msa0599260-mRNA-1
chr4_2	exon	38392870	38393078	38392870	ID=Msa0599260-mRNA-1:exon:8481;Parent=Msa0599260-mRNA-1
chr4_2	five_prime_UTR	38389077	38389292	38389077	ID=Msa0599260-mRNA-1:five_prime_utr;Parent=Msa0599260-mRNA-1
chr4_2	five_prime_UTR	38390012	38390392	38390012	ID=Msa0599260-mRNA-1:five_prime_utr;Parent=Msa0599260-mRNA-1
chr4_2	CDS	38390393	38390443	38390393	ID=Msa0599260-mRNA-1:cds;Parent=Msa0599260-mRNA-1
chr4_2	CDS	38390539	38391763	38390539	ID=Msa0599260-mRNA-1:cds;Parent=Msa0599260-mRNA-1
chr4_2	CDS	38391879	38392025	38391879	ID=Msa0599260-mRNA-1:cds;Parent=Msa0599260-mRNA-1
chr4_2	CDS	38392195	38392784	38392195	ID=Msa0599260-mRNA-1:cds;Parent=Msa0599260-mRNA-1
chr4_2	three_prime_UTR	38392785	38392849	38392785	ID=Msa0599260-mRNA-1:three_prime_utr;Parent=Msa0599260-mRNA-1
chr4_2	three_prime_UTR	38392870	38393078	38392870	ID=Msa0599260-mRNA-1:three_prime_utr;Parent=Msa0599260-mRNA-1

Gene Sequence

>Msa0599260

ATGTTTAAATTTTCTTGTTTTCATATTAACGCCCAAGGCAACAAGACAAAGAAAATAGCTAAATCTTCAACTGAAGCAATGGCCAAAGCTTTGCGAGATAGTTCCAAACATTCAGTCAGTAAAGATTCACCTATCCCTACAAGCTTAAATTCACCACTTTCAAAACAACAACCACGTAAACTAATGAATGTCTCTGAGAATGATGTTCTCGACCAGGTTTCAGTTGAGCACATTGGTAGATTAGAGGATTTCAGTAGTAAGTTTTGCATTGAATTCGACAAAAAAGTTCGCCAAACTGGGCGTATGAGGAAAAGTCAGTCGGAAAGTGTGCTTTGTCAAGGCATAACCGAGGGTGATCAAGATCTGGGGTTTTCCAAAAGCCGCTATGACTCACCAAATTCAATTGGCAGCTCTAATGATCAATCCAAAACGATTTCCAGCTTTGGGTTTCAAGTCATCCCTAGCTGTGACAATGAAGGCTCACTTTTTTCAATAGGAGATCGGACACCTTCCGATAAAGATGCTCGTGAAAATTCTGATACTCCACTATCAGGCGATCATGCTGGTGGCTCTGCTGGCTCTGCTTACCAGACTTCGGGTCCCAGTACGCCAACTTTAAGGAAGTCACGTTCTTTGCCAAATATCAAAGCTTCTACGCTTTCTTCCGCAAATAACCATGCTTTTAAACATGCATCTTCAATTTCAAGGTCTTCTGATGATCTCTGTGCTTTACGCTTGAGGAAGCAAGAGGTGTTCATCAATGTGTCTTATGATCAAATAGGGGAAACTCAAGAAAGAGAAAGTGGCATGGAAAAAACTGATAATCATATGGACAGATACTTCGATGATGGTTTTGATTCCCATCTACTTTCCGGCTTACAAAAGGACTGGACAATGCCAATTACAGATGATATAAGTGATGCTGAACCCCTTCAAGGAGATTCCTCGAGTGAGTTTCCTAACAAGGATTTTAAGGTTAAGCGGATTGAGGATTGGGTGATCGGTCTGCAGCATTGTGGACCTCCTCTCGAGGATATAACTGAATTGCCTGAATCTGTTGATCCTAAAGTTGATATTAACACCGCAAATGCCGCCGTGGCTGCCTCAGTTGTGGATCATAAGATCACTCCAGGAATGGAAACAGCTAAAAGATATATATCTTCTTTAACTGCGAATGCCACTGCAGCTCAGCTGGCGAATCATGGGTTGGCTGTGATCCCCTTTTTGAGTGCATTCGTGAGTTTGAAGGTGCTCAATTTGTCCGGAAACGCAATCGTAAGGATAACTGCTGGTACTCTTCCTCGAGGACTTCACACATTAAATTTGTCAAAAAACAATATCTCCACTATAGAGGGATTACGTGAACTTACTCGGCTTCGTGTCCTAGACCTCAGCTACAACCGTATACTAAGAATTGGACATGGTTTGGCGTCCTGTTCTTCTCTAAAGGAGCTGTACCTGGCTGGAAACAAGATTGGTGAAGTCGAGGGTCTGCACCGCCTGCTGAAACTAAGCATCCTGGATATCCGTTTTAACAAGATTTCAACTGCCAAATGTCTTGGACAACTTGCAGCCAACTATAATTCTTTGCTAGCTATCAACTTGGAAGGGAACCCTTGCCAGAAAAATGTTGGTGATGAACAAATTAAGAAATATCTGCAAAGCCTTCTTCCCCATCTTGTCTACTACAATCGGCAGCCATTCAAAGCAAATGGTTTGAAGGACGGTGGGGATCGTGCAGTTCGATTAGGAATGAATTCACAACAGTTTGATCGCAGTCTTAGAGTCGATCGCAAGTCTACAAGGAAGGGGCCATCATCTACTCGGAGGTCATCTGCTGTGTCCTCACCAAAACTGTCCAAAGGAAAGCAAGCCCAGCTCCCACCAATCAGAACCAAAACATCAACCCAAAGTCGCCATCATTTTGATTCTCCTAGCAAACCGTTGAACTTGAATTCAGGGCACTTCATGCGCAAGAGTCGCAGCGAGAGTACTCTTCTTGGAGCTTTGTAA

Protein sequence

>Msa0599260

MFKFSCFHINAQGNKTKKIAKSSTEAMAKALRDSSKHSVSKDSPIPTSLNSPLSKQQPRKLMNVSENDVLDQVSVEHIGRLEDFSSKFCIEFDKKVRQTGRMRKSQSESVLCQGITEGDQDLGFSKSRYDSPNSIGSSNDQSKTISSFGFQVIPSCDNEGSLFSIGDRTPSDKDARENSDTPLSGDHAGGSAGSAYQTSGPSTPTLRKSRSLPNIKASTLSSANNHAFKHASSISRSSDDLCALRLRKQEVFINVSYDQIGETQERESGMEKTDNHMDRYFDDGFDSHLLSGLQKDWTMPITDDISDAEPLQGDSSSEFPNKDFKVKRIEDWVIGLQHCGPPLEDITELPESVDPKVDINTANAAVAASVVDHKITPGMETAKRYISSLTANATAAQLANHGLAVIPFLSAFVSLKVLNLSGNAIVRITAGTLPRGLHTLNLSKNNISTIEGLRELTRLRVLDLSYNRILRIGHGLASCSSLKELYLAGNKIGEVEGLHRLLKLSILDIRFNKISTAKCLGQLAANYNSLLAINLEGNPCQKNVGDEQIKKYLQSLLPHLVYYNRQPFKANGLKDGGDRAVRLGMNSQQFDRSLRVDRKSTRKGPSSTRRSSAVSSPKLSKGKQAQLPPIRTKTSTQSRHHFDSPSKPLNLNSGHFMRKSRSESTLLGAL*