AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0622180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0622180 MtrunA17_Chr7g0272061 79.310 377 21 2 1 320 1 377 0.0 598
Msa0622180 MtrunA17_Chr1g0192421 68.362 354 60 3 1 305 1 351 1.20e-166 467
Msa0622180 MtrunA17_Chr4g0067711 54.802 354 110 2 1 305 1 353 6.46e-141 402
Msa0622180 MtrunA17_Chr2g0295401 55.211 355 108 3 1 305 1 354 1.05e-139 399
Msa0622180 MtrunA17_Chr7g0243571 40.798 326 128 4 52 314 53 376 5.91e-85 263
Msa0622180 MtrunA17_Chr4g0022171 40.741 324 128 4 52 313 58 379 3.10e-83 259
Msa0622180 MtrunA17_Chr4g0004971 40.924 303 125 3 56 305 43 344 1.65e-79 246
Msa0622180 MtrunA17_Chr7g0249421 59.218 179 72 1 126 304 207 384 6.24e-66 215
Msa0622180 MtrunA17_Chr7g0249421 59.155 71 29 0 52 122 57 127 2.34e-24 103
Msa0622180 MtrunA17_Chr7g0221691 57.778 180 74 2 126 305 232 409 4.48e-64 209
Msa0622180 MtrunA17_Chr7g0221691 45.977 87 47 0 50 136 88 174 1.45e-20 91.7
Msa0622180 MtrunA17_Chr8g0342431 59.649 171 66 3 135 305 206 373 5.65e-62 202
Msa0622180 MtrunA17_Chr8g0342431 52.632 76 36 0 50 125 45 120 4.67e-22 95.5
Msa0622180 MtrunA17_Chr2g0324061 51.337 187 90 1 126 312 186 371 7.93e-60 198
Msa0622180 MtrunA17_Chr2g0324061 51.562 64 30 1 55 118 55 117 3.35e-17 82.0
Msa0622180 MtrunA17_Chr1g0151781 37.966 295 131 4 60 305 38 329 1.18e-58 192
Msa0622180 MtrunA17_Chr6g0486661 29.831 295 154 7 62 305 90 382 4.76e-30 120
Msa0622180 MtrunA17_Chr4g0066371 27.636 275 150 7 73 302 68 338 7.10e-25 103
Msa0622180 MtrunA17_Chr2g0317021 35.366 246 105 11 87 304 113 332 8.64e-23 96.7
Msa0622180 MtrunA17_Chr2g0286951 28.289 304 161 10 57 307 45 344 1.39e-22 97.4
Msa0622180 MtrunA17_Chr8g0360351 33.074 257 116 12 79 305 52 282 3.12e-22 94.4
Msa0622180 MtrunA17_Chr3g0136711 31.336 217 102 7 93 281 67 264 9.72e-22 93.2
Msa0622180 MtrunA17_Chr2g0279151 39.881 168 77 7 135 302 135 278 1.17e-21 92.8
Msa0622180 MtrunA17_Chr1g0150451 30.876 217 103 6 93 281 110 307 2.10e-21 92.8
Msa0622180 MtrunA17_Chr1g0212961 28.309 272 145 10 39 281 71 321 2.00e-20 90.5
Msa0622180 MtrunA17_Chr5g0426141 39.286 168 78 7 135 302 136 279 6.79e-20 87.8
Msa0622180 MtrunA17_Chr3g0115851 40.120 167 76 7 136 302 137 279 7.21e-20 87.8
Msa0622180 MtrunA17_Chr6g0483411 36.310 168 78 4 136 290 155 306 8.51e-20 88.2
Msa0622180 MtrunA17_Chr4g0050381 37.952 166 79 7 137 302 133 274 3.30e-19 85.9
Msa0622180 MtrunA17_Chr5g0396731 42.308 130 58 4 137 266 188 300 5.52e-19 86.3
Msa0622180 MtrunA17_Chr3g0105371 31.655 278 135 12 62 308 89 342 4.99e-18 83.6
Msa0622180 MtrunA17_Chr3g0126511 28.090 267 136 10 65 302 99 338 5.75e-18 83.6
Msa0622180 MtrunA17_Chr4g0051661 29.918 244 118 12 88 304 180 397 6.20e-18 83.6
Msa0622180 MtrunA17_Chr3g0112291 28.339 307 157 10 3 264 33 321 2.61e-17 82.0
Msa0622180 MtrunA17_Chr1g0171571 32.022 178 98 7 136 312 497 652 6.86e-16 78.2
Msa0622180 MtrunA17_Chr5g0430481 28.623 276 154 11 54 304 117 374 7.02e-16 77.4
Msa0622180 MtrunA17_Chr5g0435721 39.844 128 60 4 137 264 309 419 7.27e-16 78.2
Msa0622180 MtrunA17_Chr8g0371321 30.144 209 94 10 136 304 157 353 1.11e-15 77.0
Msa0622180 MtrunA17_Chr3g0105351 30.657 274 135 12 62 304 65 314 1.26e-15 76.3
Msa0622180 MtrunA17_Chr4g0070871 32.075 212 86 8 91 263 253 445 1.62e-15 77.0
Msa0622180 MtrunA17_Chr1g0213351 27.645 293 129 13 91 310 80 362 1.03e-14 74.3
Msa0622180 MtrunA17_Chr1g0158781 40.164 122 56 4 143 264 226 330 1.06e-14 74.3
Msa0622180 MtrunA17_Chr3g0135961 29.200 250 105 10 59 263 262 484 2.14e-14 73.6
Msa0622180 MtrunA17_Chr1g0211251 32.195 205 91 9 138 304 161 355 5.06e-14 72.0
Msa0622180 MtrunA17_Chr3g0089711 27.393 303 129 13 88 312 65 354 1.01e-13 71.2
Msa0622180 MtrunA17_Chr3g0089721 27.393 303 129 13 88 312 65 354 1.30e-13 70.9
Msa0622180 MtrunA17_Chr5g0407161 29.808 208 89 7 91 264 124 308 4.08e-13 69.3
Msa0622180 MtrunA17_Chr3g0138281 26.923 260 117 12 91 307 121 350 5.80e-13 68.9
Msa0622180 MtrunA17_Chr3g0089691 30.275 218 99 9 137 312 62 268 6.79e-13 68.2
Msa0622180 MtrunA17_Chr7g0256751 27.668 253 114 12 88 282 66 307 8.99e-13 68.6
Msa0622180 MtrunA17_Chr4g0065141 25.391 256 140 10 85 307 118 355 1.19e-12 68.2
Msa0622180 MtrunA17_Chr1g0150781 37.600 125 61 4 139 263 381 488 2.96e-12 67.4
Msa0622180 MtrunA17_Chr4g0067301 32.051 156 73 4 135 266 286 432 5.86e-12 66.2
Msa0622180 MtrunA17_Chr8g0389921 29.327 208 93 6 91 263 198 386 9.57e-12 65.5
Msa0622180 MtrunA17_Chr3g0144111 29.348 184 98 5 91 263 27 189 1.15e-11 63.9
Msa0622180 MtrunA17_Chr1g0194191 32.090 134 67 5 136 264 198 312 3.50e-11 63.5
Msa0622180 MtrunA17_Chr1g0150071 30.597 134 66 4 139 264 173 287 7.11e-11 62.4
Msa0622180 MtrunA17_Chr1g0209451 28.638 213 117 11 111 307 107 300 8.12e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0622180 AT3G05640.2 66.097 351 72 3 1 305 1 350 5.47e-167 469
Msa0622180 AT3G05640.1 66.097 351 72 3 1 305 1 350 5.47e-167 469
Msa0622180 AT3G05640.3 66.097 351 72 3 1 305 1 350 5.47e-167 469
Msa0622180 AT5G27930.1 62.994 354 79 4 1 303 1 353 8.59e-162 456
Msa0622180 AT5G27930.2 62.994 354 79 4 1 303 1 353 8.59e-162 456
Msa0622180 AT5G27930.3 62.252 302 62 4 1 251 1 301 2.39e-132 379
Msa0622180 AT3G16800.2 52.286 350 123 3 1 306 1 350 1.38e-124 361
Msa0622180 AT3G16800.1 52.286 350 123 3 1 306 1 350 1.38e-124 361
Msa0622180 AT3G16800.3 52.286 350 123 3 1 306 1 350 1.38e-124 361
Msa0622180 AT3G16800.4 54.510 255 74 2 94 306 1 255 7.69e-93 276
Msa0622180 AT3G16800.6 54.510 255 74 2 94 306 1 255 7.69e-93 276
Msa0622180 AT1G03590.1 41.290 310 120 4 55 304 55 362 1.88e-78 247
Msa0622180 AT5G01700.2 42.071 309 128 5 52 311 39 345 4.91e-76 238
Msa0622180 AT5G01700.1 43.051 295 117 5 66 311 4 296 2.08e-75 235
Msa0622180 AT4G32950.1 42.105 285 131 4 59 311 41 323 5.91e-71 223
Msa0622180 AT1G79630.6 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa0622180 AT1G79630.3 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa0622180 AT1G79630.4 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa0622180 AT1G79630.5 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa0622180 AT1G79630.2 56.667 180 77 1 126 305 152 330 6.16e-69 221
Msa0622180 AT1G79630.2 50.000 52 26 0 81 132 18 69 3.00e-11 64.3
Msa0622180 AT1G79630.7 56.667 180 77 1 126 305 152 330 6.16e-69 221
Msa0622180 AT1G79630.7 50.000 52 26 0 81 132 18 69 3.00e-11 64.3
Msa0622180 AT1G79630.1 56.667 180 77 1 126 305 222 400 1.85e-68 222
Msa0622180 AT1G79630.1 45.783 83 45 0 50 132 57 139 2.81e-20 91.7
Msa0622180 AT1G79630.8 56.667 180 77 1 126 305 218 396 1.94e-68 222
Msa0622180 AT1G79630.8 45.783 83 45 0 50 132 53 135 3.55e-20 91.3
Msa0622180 AT1G16220.1 55.556 180 79 1 126 305 199 377 2.74e-66 216
Msa0622180 AT1G16220.1 45.122 82 45 0 50 131 54 135 1.65e-19 89.4
Msa0622180 AT5G26010.1 42.123 292 128 5 58 310 41 330 2.98e-65 209
Msa0622180 AT3G02750.2 56.983 179 76 1 126 304 216 393 1.70e-63 209
Msa0622180 AT3G02750.2 39.450 109 63 1 14 122 21 126 4.36e-23 99.8
Msa0622180 AT3G02750.1 56.983 179 76 1 126 304 216 393 1.70e-63 209
Msa0622180 AT3G02750.1 39.450 109 63 1 14 122 21 126 4.36e-23 99.8
Msa0622180 AT5G36250.1 51.397 179 86 1 126 304 207 384 3.65e-61 201
Msa0622180 AT5G36250.1 44.444 108 51 2 50 148 57 164 6.32e-22 95.9
Msa0622180 AT4G03415.1 54.190 179 81 1 135 313 204 381 1.18e-60 201
Msa0622180 AT4G03415.1 47.826 69 35 1 63 131 70 137 2.83e-16 79.7
Msa0622180 AT4G03415.3 54.190 179 81 1 135 313 204 381 1.18e-60 201
Msa0622180 AT4G03415.3 47.826 69 35 1 63 131 70 137 2.83e-16 79.7
Msa0622180 AT4G03415.2 54.190 179 81 1 135 313 204 381 1.18e-60 201
Msa0622180 AT4G03415.2 47.826 69 35 1 63 131 70 137 2.83e-16 79.7
Msa0622180 AT5G36250.2 48.187 193 85 2 126 304 207 398 1.70e-57 192
Msa0622180 AT5G36250.2 44.444 108 51 2 50 148 57 164 9.04e-22 95.9
Msa0622180 AT3G02750.3 47.664 214 76 2 126 304 216 428 2.17e-56 191
Msa0622180 AT3G02750.3 37.391 115 69 1 14 128 21 132 8.80e-23 99.0
Msa0622180 AT3G16800.5 45.205 219 76 3 1 175 1 219 3.38e-56 182
Msa0622180 AT4G32950.2 43.111 225 94 4 59 251 41 263 2.75e-53 176
Msa0622180 AT5G36250.3 53.521 142 64 2 126 266 207 347 4.21e-45 157
Msa0622180 AT5G36250.3 44.037 109 52 2 50 149 57 165 4.68e-22 95.5
Msa0622180 AT2G20050.2 30.847 295 146 9 62 304 109 397 3.10e-30 121
Msa0622180 AT2G20050.1 30.847 295 146 9 62 304 109 397 3.50e-30 121
Msa0622180 AT2G20630.1 33.607 244 108 10 87 302 59 276 1.08e-24 101
Msa0622180 AT2G20630.2 33.884 242 106 10 88 301 60 275 3.33e-24 100
Msa0622180 AT3G06270.1 29.304 273 137 8 50 269 40 309 3.48e-24 101
Msa0622180 AT4G28400.3 33.198 247 111 12 87 305 41 261 5.52e-24 99.0
Msa0622180 AT4G28400.1 34.167 240 104 12 94 305 70 283 6.12e-24 99.4
Msa0622180 AT4G31750.1 31.390 223 103 8 93 287 66 266 2.36e-21 92.8
Msa0622180 AT4G31750.3 31.390 223 103 8 93 287 66 266 2.36e-21 92.8
Msa0622180 AT4G31750.2 31.390 223 103 8 93 287 66 266 2.39e-21 92.8
Msa0622180 AT5G24940.1 31.301 246 108 8 91 304 64 280 7.06e-21 93.2
Msa0622180 AT5G10740.1 31.174 247 109 8 91 305 64 281 1.12e-20 91.7
Msa0622180 AT5G10740.2 31.174 247 109 8 91 305 64 281 1.12e-20 91.7
Msa0622180 AT1G34750.2 33.333 204 98 9 115 304 102 281 6.13e-19 85.5
Msa0622180 AT1G34750.1 33.333 204 98 9 115 304 102 281 6.13e-19 85.5
Msa0622180 AT1G34750.3 33.333 204 98 9 115 304 102 281 6.13e-19 85.5
Msa0622180 AT5G59220.1 31.776 214 87 7 91 264 148 342 9.30e-19 86.7
Msa0622180 AT5G53140.1 28.221 326 177 13 9 305 52 349 1.24e-18 86.3
Msa0622180 AT5G53140.2 28.221 326 177 13 9 305 52 349 1.24e-18 86.3
Msa0622180 AT1G78200.5 31.746 252 118 12 80 302 9 235 1.72e-18 83.6
Msa0622180 AT1G78200.4 31.746 252 118 12 80 302 9 235 1.72e-18 83.6
Msa0622180 AT1G78200.1 31.746 252 118 12 80 302 54 280 2.46e-18 84.0
Msa0622180 AT1G78200.3 31.746 252 118 12 80 302 54 280 2.46e-18 84.0
Msa0622180 AT1G78200.2 31.746 252 118 12 80 302 54 280 2.46e-18 84.0
Msa0622180 AT3G15260.2 37.952 166 79 7 137 302 145 286 3.32e-18 83.6
Msa0622180 AT3G15260.1 37.952 166 79 7 137 302 145 286 3.32e-18 83.6
Msa0622180 AT1G07160.1 30.325 277 135 13 62 304 124 376 7.31e-18 84.0
Msa0622180 AT1G07430.1 40.625 128 59 4 137 264 244 354 1.29e-17 83.6
Msa0622180 AT2G40180.1 29.371 286 139 13 58 305 126 386 1.40e-17 83.2
Msa0622180 AT1G43900.1 29.508 244 122 9 65 279 128 350 1.42e-17 82.8
Msa0622180 AT3G51370.2 30.435 207 96 9 136 304 72 268 1.01e-16 79.3
Msa0622180 AT3G55050.1 31.731 208 88 10 139 305 165 359 1.04e-16 80.5
Msa0622180 AT3G55050.2 31.731 208 88 10 139 305 165 359 1.04e-16 80.5
Msa0622180 AT2G25620.1 28.462 260 131 11 91 315 128 367 1.53e-16 80.1
Msa0622180 AT2G25620.2 28.462 260 131 11 91 315 128 367 1.53e-16 80.1
Msa0622180 AT3G51370.1 30.435 207 96 8 136 304 157 353 2.34e-16 79.3
Msa0622180 AT3G51370.3 30.435 207 96 8 136 304 157 353 2.34e-16 79.3
Msa0622180 AT2G34740.1 27.652 264 138 12 67 302 94 332 6.21e-16 77.8
Msa0622180 AT3G11410.1 39.370 127 60 4 137 263 232 341 1.02e-15 77.8
Msa0622180 AT2G30020.1 28.623 276 140 12 62 304 141 392 1.52e-15 77.0
Msa0622180 AT4G26080.1 31.532 222 97 8 78 263 159 361 2.12e-15 77.0
Msa0622180 AT1G22280.1 33.929 168 87 7 135 302 135 278 3.78e-15 74.7
Msa0622180 AT1G22280.3 34.524 168 86 8 135 302 141 284 4.04e-15 74.7
Msa0622180 AT5G57050.1 31.754 211 87 8 91 263 160 351 2.96e-14 73.6
Msa0622180 AT5G57050.2 31.754 211 87 8 91 263 120 311 3.22e-14 73.2
Msa0622180 AT3G51470.2 26.115 314 153 13 50 315 12 294 5.20e-14 72.0
Msa0622180 AT5G66080.2 29.902 204 95 9 139 304 59 252 5.39e-14 71.6
Msa0622180 AT1G67820.2 29.528 254 132 10 49 275 75 308 5.73e-14 72.4
Msa0622180 AT1G67820.1 29.528 254 132 10 49 275 111 344 6.83e-14 72.4
Msa0622180 AT3G51470.1 26.115 314 153 13 50 315 56 338 8.62e-14 71.6
Msa0622180 AT5G66080.1 29.902 204 95 9 139 304 163 356 9.56e-14 71.6
Msa0622180 AT3G12620.2 31.373 204 92 9 139 304 164 357 2.12e-13 70.9
Msa0622180 AT3G12620.1 31.373 204 92 9 139 304 164 357 2.12e-13 70.9
Msa0622180 AT1G47380.1 30.137 219 120 11 112 315 104 304 3.16e-13 70.5
Msa0622180 AT1G18030.1 31.447 159 84 5 137 282 186 332 4.31e-13 69.7
Msa0622180 AT3G62260.3 30.319 188 103 8 119 300 90 255 4.62e-13 68.9
Msa0622180 AT4G28400.2 31.746 189 82 10 94 254 70 239 4.80e-13 68.6
Msa0622180 AT4G38520.2 30.244 205 93 10 139 304 162 355 4.92e-13 69.7
Msa0622180 AT4G38520.1 30.244 205 93 10 139 304 162 355 4.92e-13 69.7
Msa0622180 AT1G17550.1 37.903 124 60 3 139 262 339 445 5.08e-13 70.1
Msa0622180 AT1G17550.2 37.903 124 60 3 139 262 339 445 5.08e-13 70.1
Msa0622180 AT5G51760.2 40.000 130 58 5 134 263 212 321 5.33e-13 69.3
Msa0622180 AT5G51760.1 40.000 130 58 5 134 263 242 351 6.21e-13 69.3
Msa0622180 AT5G02760.1 28.230 209 94 8 139 306 153 346 6.24e-13 69.3
Msa0622180 AT1G48040.1 32.065 184 96 7 136 312 197 358 1.06e-12 68.6
Msa0622180 AT2G40860.1 30.857 175 98 7 139 312 504 656 1.15e-12 68.9
Msa0622180 AT3G62260.1 29.474 190 106 7 119 302 179 346 1.20e-12 68.6
Msa0622180 AT2G40860.4 30.857 175 98 7 139 312 406 558 1.23e-12 68.9
Msa0622180 AT3G62260.2 29.474 190 106 7 119 302 180 347 1.26e-12 68.6
Msa0622180 AT2G33700.1 27.106 273 127 11 39 263 58 306 1.99e-12 67.8
Msa0622180 AT1G72770.4 39.850 133 47 6 139 263 339 446 2.47e-12 67.8
Msa0622180 AT1G72770.1 39.850 133 47 6 139 263 339 446 2.47e-12 67.8
Msa0622180 AT1G72770.3 39.850 133 47 6 139 263 339 446 2.47e-12 67.8
Msa0622180 AT2G34740.2 26.271 236 127 11 67 273 108 325 4.11e-12 66.6
Msa0622180 AT3G17250.1 24.074 270 135 7 89 312 156 401 4.70e-12 66.6
Msa0622180 AT2G34740.3 26.271 236 127 11 67 273 90 307 4.88e-12 66.2
Msa0622180 AT5G06750.3 25.532 282 140 11 90 304 81 359 6.04e-12 66.2
Msa0622180 AT5G06750.1 25.532 282 140 11 90 304 81 359 6.04e-12 66.2
Msa0622180 AT1G18030.2 33.071 127 64 4 137 254 186 300 6.67e-12 65.9
Msa0622180 AT5G06750.2 28.141 199 110 7 138 304 80 277 1.94e-11 64.3
Msa0622180 AT1G68410.4 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa0622180 AT1G68410.3 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa0622180 AT1G68410.1 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa0622180 AT1G68410.2 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa0622180 AT1G09160.2 29.268 205 94 10 93 260 70 260 3.02e-11 64.3
Msa0622180 AT1G09160.1 29.268 205 94 10 93 260 70 260 3.02e-11 64.3
Msa0622180 AT1G34750.4 32.099 162 79 8 107 254 94 238 8.83e-11 61.6
Msa0622180 AT2G25070.1 30.597 134 66 4 139 264 173 287 9.98e-11 62.4
Msa0622180 AT2G25070.2 30.597 134 66 4 139 264 173 287 9.98e-11 62.4

Find 91 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGATTAAATTATAGGAATT+TGG 0.150752 4_2:+74532862 Msa0622180:intron
GATTATTGTATTAAAGATTA+TGG 0.199525 4_2:+74533983 Msa0622180:CDS
GGTTCCTTTCTGACCTCTTT+TGG 0.202885 4_2:-74532515 None:intergenic
CTTGCCATGTCAAGGGCTTT+TGG 0.208056 4_2:+74533959 Msa0622180:CDS
CATTGACTCTCTTACTCTTT+TGG 0.217341 4_2:-74532950 None:intergenic
AAGTACTAACGAGGTAAATT+TGG 0.252923 4_2:-74533761 None:intergenic
ATCCTCTATGTTCAATGGTT+TGG 0.312680 4_2:+74532333 Msa0622180:CDS
CGAGAAAGAAAAGATGTAAA+AGG 0.318796 4_2:-74531179 None:intergenic
GTCTATTTAACAGTTTCAAT+TGG 0.327269 4_2:-74534762 None:intergenic
AATGTCTACTGCTTCTTGAT+TGG 0.329653 4_2:-74534586 None:intergenic
TCTGGCACTATTAATGTTGA+TGG 0.338334 4_2:+74532466 Msa0622180:CDS
GCTATCTTGCTCAGGGGAAA+TGG 0.343840 4_2:-74534739 None:intergenic
TCTCAGATTCACCTTCATTA+AGG 0.358244 4_2:+74531263 Msa0622180:five_prime_UTR
CATTTATCCTCTATGTTCAA+TGG 0.363846 4_2:+74532328 Msa0622180:CDS
ATATTCTGCGGTATATTTGA+TGG 0.364778 4_2:+74532901 Msa0622180:CDS
ATATTCCTCTGAGTTACTTC+AGG 0.378333 4_2:-74534019 None:intergenic
ATCAGACGATGATGGAAGTT+TGG 0.389577 4_2:+74533709 Msa0622180:CDS
TCACAAAATTGAGGATTTGA+TGG 0.399038 4_2:+74532303 Msa0622180:exon
CAAGGTCTTTGCTATGCAAT+TGG 0.407044 4_2:+74532974 Msa0622180:CDS
TTTGTTGTGCTTGCCACTGA+TGG 0.408199 4_2:+74534058 Msa0622180:CDS
TCCGTAGGCCATAAATAAAT+AGG 0.409749 4_2:+74531090 None:intergenic
CACCATGCAACAGTCTTGAT+TGG 0.411872 4_2:-74532536 None:intergenic
TCAATTGGGCTATCTTGCTC+AGG 0.413365 4_2:-74534747 None:intergenic
TATTTGATGGACACGGGCCA+TGG 0.420048 4_2:+74532914 Msa0622180:CDS
CTATCTTGCTCAGGGGAAAT+GGG 0.431506 4_2:-74534738 None:intergenic
ATATGACCTTTGGTCCAGTT+AGG 0.431557 4_2:+74534819 Msa0622180:three_prime_UTR
TGTTGGGTTCATATGACCTT+TGG 0.438991 4_2:+74534809 Msa0622180:three_prime_UTR
TCTATTTAACAGTTTCAATT+GGG 0.444557 4_2:-74534761 None:intergenic
GCCCTTGACATGGCAAGTCC+TGG 0.445151 4_2:-74533953 None:intergenic
AGCAAAATCAGCTAAACGTT+TGG 0.451570 4_2:+74534629 Msa0622180:CDS
TGGTGACTCTCGTGCAGTAC+TGG 0.463725 4_2:+74533679 Msa0622180:CDS
TGAACCCAACATCATGAAGT+TGG 0.468936 4_2:-74534797 None:intergenic
AAGCCCAACTTCATGATGTT+GGG 0.471507 4_2:+74534793 Msa0622180:three_prime_UTR
ATAAATAAATAGGATCAAAC+AGG 0.472624 4_2:+74531100 None:intergenic
TTGTTGTGCTTGCCACTGAT+GGG 0.475803 4_2:+74534059 Msa0622180:CDS
GTTGTGCTGAGTTGAGGAAC+AGG 0.476515 4_2:-74531232 None:intergenic
TATAACATAGACACATTAAG+AGG 0.479566 4_2:+74532272 Msa0622180:five_prime_UTR
CAGTGGCAAGCACAACAAAC+TGG 0.480296 4_2:-74534054 None:intergenic
TAAGCCCAACTTCATGATGT+TGG 0.481030 4_2:+74534792 Msa0622180:three_prime_UTR
AACCAGGAGTGCATAGGGTC+TGG 0.482895 4_2:+74533912 Msa0622180:CDS
CACAAAATTGAGGATTTGAT+GGG 0.483155 4_2:+74532304 Msa0622180:exon
AACCAGACCCTATGCACTCC+TGG 0.483257 4_2:-74533914 None:intergenic
CGCAGAATATCATGTCCTCT+TGG 0.484795 4_2:-74532888 None:intergenic
ATTTGATGGACACGGGCCAT+GGG 0.489246 4_2:+74532915 Msa0622180:CDS
AATAGCAGAAATATCATCAA+TGG 0.495485 4_2:-74534697 None:intergenic
TATGACCTTTGGTCCAGTTA+GGG 0.496034 4_2:+74534820 Msa0622180:three_prime_UTR
CTCCAGGACTTGCCATGTCA+AGG 0.496930 4_2:+74533951 Msa0622180:CDS
TTCTCCAAAAGAGGTCAGAA+AGG 0.500364 4_2:+74532511 Msa0622180:CDS
AGTCCTGGAGACTCTACATC+TGG 0.508363 4_2:-74533938 None:intergenic
TGATGAACCAGGAGTGCATA+GGG 0.509549 4_2:+74533907 Msa0622180:CDS
GAACCCAACATCATGAAGTT+GGG 0.509814 4_2:-74534796 None:intergenic
TTACCAGATGTAGAGTCTCC+AGG 0.526827 4_2:+74533935 Msa0622180:CDS
TGCATGGAAACGCAAGAGAA+GGG 0.527483 4_2:+74534668 Msa0622180:CDS
GAGAGAATAATTCAGTGTCA+AGG 0.530299 4_2:+74533866 Msa0622180:CDS
ATCAAGACTGTTGCATGGTG+TGG 0.533904 4_2:+74532539 Msa0622180:CDS
CAATTGGGCTATCTTGCTCA+GGG 0.538157 4_2:-74534746 None:intergenic
GAAAGTATATCACAAAATTG+AGG 0.554475 4_2:+74532294 Msa0622180:five_prime_UTR
GAGAGAAGCTGTTGAAGCAA+TGG 0.555010 4_2:+74532405 Msa0622180:CDS
TGCGGTATATTTGATGGACA+CGG 0.560385 4_2:+74532907 Msa0622180:CDS
TCCAGGACTTGCCATGTCAA+GGG 0.561968 4_2:+74533952 Msa0622180:CDS
GCTACAACATCAGACGATGA+TGG 0.562423 4_2:+74533701 Msa0622180:CDS
ATGCATGGAAACGCAAGAGA+AGG 0.563663 4_2:+74534667 Msa0622180:CDS
AAAATCAGCTAAACGTTTGG+TGG 0.569397 4_2:+74534632 Msa0622180:CDS
TGCCAAACCATTGAACATAG+AGG 0.573400 4_2:-74532335 None:intergenic
CAATTGCATAGCAAAGACCT+TGG 0.578108 4_2:-74532973 None:intergenic
GAAATTCTGTGAGATGTGAA+AGG 0.584077 4_2:+74532383 Msa0622180:CDS
GCGGTATATTTGATGGACAC+GGG 0.590895 4_2:+74532908 Msa0622180:CDS
CTTGCTCAGGGGAAATGGGA+GGG 0.592417 4_2:-74534734 None:intergenic
GTAACAGTTGTGCTGAGTTG+AGG 0.593859 4_2:-74531238 None:intergenic
CAATTAAGAGACCTTAATGA+AGG 0.594485 4_2:-74531274 None:intergenic
AGAGAATAATTCAGTGTCAA+GGG 0.594886 4_2:+74533867 Msa0622180:CDS
TCTTGCTCAGGGGAAATGGG+AGG 0.601493 4_2:-74534735 None:intergenic
TGTTGTGCTTGCCACTGATG+GGG 0.603053 4_2:+74534060 Msa0622180:CDS
TTCTACCCTAACTGGACCAA+AGG 0.605758 4_2:-74534825 None:intergenic
TTGGCTACAAAGTGACCCCA+TGG 0.606377 4_2:-74532931 None:intergenic
GAGTAAGAGAGTCAATGCCA+AGG 0.613440 4_2:+74532956 Msa0622180:CDS
ATGATGAACCAGGAGTGCAT+AGG 0.615902 4_2:+74533906 Msa0622180:CDS
ATCTCCAAAAGCCCTTGACA+TGG 0.615912 4_2:-74533963 None:intergenic
TCAAGACTGTTGCATGGTGT+GGG 0.621717 4_2:+74532540 Msa0622180:CDS
TGGAATGTGCAGTACATGCA+TGG 0.626814 4_2:+74534652 Msa0622180:CDS
AACCAATCAAGACTGTTGCA+TGG 0.651730 4_2:+74532534 Msa0622180:CDS
GCATGGAAACGCAAGAGAAG+GGG 0.652422 4_2:+74534669 Msa0622180:CDS
ACAAAATTGAGGATTTGATG+GGG 0.663664 4_2:+74532305 Msa0622180:exon
TTCTGTTTAAATGATGAACC+AGG 0.665974 4_2:+74533896 Msa0622180:CDS
CAAGATACTTACCCCATCAG+TGG 0.666268 4_2:-74534071 None:intergenic
CAAGAGGACATGATATTCTG+CGG 0.676913 4_2:+74532889 Msa0622180:CDS
ATAGGAATTTGGATGCCAAG+AGG 0.678718 4_2:+74532873 Msa0622180:intron
TTTGATGGACACGGGCCATG+GGG 0.686895 4_2:+74532916 Msa0622180:CDS
AGACTGTTGCATGGTGTGGG+AGG 0.695512 4_2:+74532543 Msa0622180:CDS
AATTGGGCTATCTTGCTCAG+GGG 0.712499 4_2:-74534745 None:intergenic
CAGTACCTGAAGTAACTCAG+AGG 0.714319 4_2:+74534014 Msa0622180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAAATATAACAAAA+TGG + chr4_2:74531326-74531345 Msa0622180:intron 10.0%
!! ACGAATAAAAATTATTAATA+TGG + chr4_2:74532146-74532165 Msa0622180:intron 10.0%
!! AAAAATAAAGTCTTCATAAA+TGG + chr4_2:74531145-74531164 Msa0622180:five_prime_UTR 15.0%
!! AAAAGAAGAAAAATAATGAA+TGG - chr4_2:74531305-74531324 None:intergenic 15.0%
!!! GTATTTTTCCTATTTTTATT+TGG + chr4_2:74531348-74531367 Msa0622180:intron 15.0%
!!! AAATATGTCACTTTTTTTTA+AGG + chr4_2:74531512-74531531 Msa0622180:intron 15.0%
!! CAAATTTATATAGAAGAAAA+AGG - chr4_2:74532210-74532229 None:intergenic 15.0%
!! ATATCAAAGACAAATAAAAT+GGG - chr4_2:74532790-74532809 None:intergenic 15.0%
!! TATATCAAAGACAAATAAAA+TGG - chr4_2:74532791-74532810 None:intergenic 15.0%
!! TTTGATATAAAATTCAATTC+TGG + chr4_2:74532802-74532821 Msa0622180:intron 15.0%
!! GATTATTGTATTAAAGATTA+TGG + chr4_2:74533983-74534002 Msa0622180:CDS 15.0%
!! AAAATTGAGATTAAATATGT+TGG + chr4_2:74534254-74534273 Msa0622180:intron 15.0%
!!! TTATCTATTTATTTGTTATC+TGG - chr4_2:74534354-74534373 None:intergenic 15.0%
!! ACAAATAAATAAATAATCAC+CGG + chr4_2:74534414-74534433 Msa0622180:intron 15.0%
!!! AGTTAATTATGTTTTTAGTT+TGG - chr4_2:74534488-74534507 None:intergenic 15.0%
!! TACAAACACCAAATAAAAAT+AGG - chr4_2:74531359-74531378 None:intergenic 20.0%
!!! ATATGATTTTTGATTTTCTC+AGG + chr4_2:74531571-74531590 Msa0622180:intron 20.0%
!! TATATATTAAGTTCCATAAG+AGG - chr4_2:74531745-74531764 None:intergenic 20.0%
!! TTGAATATACTATATGCAAA+TGG - chr4_2:74531928-74531947 None:intergenic 20.0%
!! TGCATATTGAAAATAGATTA+AGG - chr4_2:74532239-74532258 None:intergenic 20.0%
!!! TGGGTTGATTATAAATATAT+AGG - chr4_2:74532771-74532790 None:intergenic 20.0%
!! TAATTTAATCGTCAATAGAT+AGG - chr4_2:74532854-74532873 None:intergenic 20.0%
!! TCTATTGACGATTAAATTAT+AGG + chr4_2:74532855-74532874 Msa0622180:intron 20.0%
!! ACGATTAAATTATAGGAATT+TGG + chr4_2:74532862-74532881 Msa0622180:intron 20.0%
!! AACAACAAAGATTCAATATA+TGG + chr4_2:74533079-74533098 Msa0622180:intron 20.0%
!!! TTTATTTTATAATGGTTTGC+AGG + chr4_2:74533631-74533650 Msa0622180:intron 20.0%
!!! TTATTTTATAATGGTTTGCA+GGG + chr4_2:74533632-74533651 Msa0622180:intron 20.0%
!!! AATCATAGTAACTAGTTTAT+AGG - chr4_2:74534098-74534117 None:intergenic 20.0%
!! TTAAATTTATTGCTGAAATG+TGG + chr4_2:74534509-74534528 Msa0622180:intron 20.0%
!! TCTATTTAACAGTTTCAATT+GGG - chr4_2:74534764-74534783 None:intergenic 20.0%
!!! ATGAAGACTTTATTTTTGTC+TGG - chr4_2:74531142-74531161 None:intergenic 25.0%
! CTTACTTTGATGAATTTAAG+AGG + chr4_2:74531433-74531452 Msa0622180:intron 25.0%
!! TTTTCATGATACTGAATCAT+AGG + chr4_2:74531625-74531644 Msa0622180:intron 25.0%
! ATGGATAAAGAAATCAGAAA+AGG - chr4_2:74531893-74531912 None:intergenic 25.0%
! TATAACATAGACACATTAAG+AGG + chr4_2:74532272-74532291 Msa0622180:five_prime_UTR 25.0%
!! GAAAGTATATCACAAAATTG+AGG + chr4_2:74532294-74532313 Msa0622180:five_prime_UTR 25.0%
!! AGATCATTTTCACTAAAGAA+AGG + chr4_2:74532358-74532377 Msa0622180:CDS 25.0%
! GATATAAAATTCAATTCTGG+TGG + chr4_2:74532805-74532824 Msa0622180:intron 25.0%
!! TTTTAATGAATTTCTCCCTA+CGG - chr4_2:74533242-74533261 None:intergenic 25.0%
! TCTGCTAACTAGTATATATA+CGG - chr4_2:74533273-74533292 None:intergenic 25.0%
! ATTTGTTCATGTTACAAGTT+TGG - chr4_2:74533478-74533497 None:intergenic 25.0%
!!! TATTTTATAATGGTTTGCAG+GGG + chr4_2:74533633-74533652 Msa0622180:intron 25.0%
!!! AATAAATAGAAGTACTAACG+AGG - chr4_2:74533773-74533792 None:intergenic 25.0%
!! GAGTTTATTGTTGTCAATTT+TGG - chr4_2:74534218-74534237 None:intergenic 25.0%
! TGAGACATTTAGATTATGAT+TGG + chr4_2:74534312-74534331 Msa0622180:intron 25.0%
! AATAGCAGAAATATCATCAA+TGG - chr4_2:74534700-74534719 None:intergenic 25.0%
! GTCTATTTAACAGTTTCAAT+TGG - chr4_2:74534765-74534784 None:intergenic 25.0%
CGAGAAAGAAAAGATGTAAA+AGG - chr4_2:74531182-74531201 None:intergenic 30.0%
CAATTAAGAGACCTTAATGA+AGG - chr4_2:74531277-74531296 None:intergenic 30.0%
TTAACACAGTAGTTCAGTAA+TGG + chr4_2:74531834-74531853 Msa0622180:intron 30.0%
ATAAAGAAATCAGAAAAGGC+TGG - chr4_2:74531889-74531908 None:intergenic 30.0%
CAAATGGATCAAACTGAATA+TGG - chr4_2:74531912-74531931 None:intergenic 30.0%
ATTGTCTTCATGTTAAGTCA+CGG + chr4_2:74532077-74532096 Msa0622180:intron 30.0%
TTCTTCAATAGCACATAGAA+AGG - chr4_2:74532174-74532193 None:intergenic 30.0%
TATATAGAAGAAAAAGGAGC+AGG - chr4_2:74532204-74532223 None:intergenic 30.0%
TCACAAAATTGAGGATTTGA+TGG + chr4_2:74532303-74532322 Msa0622180:exon 30.0%
CACAAAATTGAGGATTTGAT+GGG + chr4_2:74532304-74532323 Msa0622180:exon 30.0%
ACAAAATTGAGGATTTGATG+GGG + chr4_2:74532305-74532324 Msa0622180:exon 30.0%
CATTTATCCTCTATGTTCAA+TGG + chr4_2:74532328-74532347 Msa0622180:CDS 30.0%
TTGAAGGTTAAACAAACTCA+CGG - chr4_2:74532667-74532686 None:intergenic 30.0%
TACATGTTTGTATACACGTT+GGG - chr4_2:74532731-74532750 None:intergenic 30.0%
! ACATGTACTCGTTACATTTT+TGG + chr4_2:74532744-74532763 Msa0622180:intron 30.0%
ATATTCTGCGGTATATTTGA+TGG + chr4_2:74532901-74532920 Msa0622180:CDS 30.0%
AACAATATAGTTGCGATGTT+AGG + chr4_2:74533302-74533321 Msa0622180:intron 30.0%
ACAATATAGTTGCGATGTTA+GGG + chr4_2:74533303-74533322 Msa0622180:intron 30.0%
!! TATGTCTTTGAAATAAGTGC+TGG + chr4_2:74533331-74533350 Msa0622180:intron 30.0%
!! ATGTCTTTGAAATAAGTGCT+GGG + chr4_2:74533332-74533351 Msa0622180:intron 30.0%
GTAATCCAATTCCATCAAAT+AGG - chr4_2:74533375-74533394 None:intergenic 30.0%
GTTACAAGTTTGGTAAATGT+AGG - chr4_2:74533468-74533487 None:intergenic 30.0%
! ACATGAACAAATTGATGACA+TGG + chr4_2:74533486-74533505 Msa0622180:intron 30.0%
AACTTCACTGATATATTGGT+TGG - chr4_2:74533584-74533603 None:intergenic 30.0%
CAAGAACTTCACTGATATAT+TGG - chr4_2:74533588-74533607 None:intergenic 30.0%
!! ATTTTATAATGGTTTGCAGG+GGG + chr4_2:74533634-74533653 Msa0622180:intron 30.0%
AAGTACTAACGAGGTAAATT+TGG - chr4_2:74533764-74533783 None:intergenic 30.0%
TAATTGCATAATTGCAGAAG+AGG + chr4_2:74533841-74533860 Msa0622180:intron 30.0%
AGAGAATAATTCAGTGTCAA+GGG + chr4_2:74533867-74533886 Msa0622180:CDS 30.0%
TTCTGTTTAAATGATGAACC+AGG + chr4_2:74533896-74533915 Msa0622180:CDS 30.0%
! TTTTATCATTCTACCCTAAC+TGG - chr4_2:74534836-74534855 None:intergenic 30.0%
TCTCAGATTCACCTTCATTA+AGG + chr4_2:74531263-74531282 Msa0622180:five_prime_UTR 35.0%
!! CGGTGTATTTGTTTCTGAAT+TGG - chr4_2:74531696-74531715 None:intergenic 35.0%
! ATACACCGTTTCATTTTGTG+TGG + chr4_2:74531708-74531727 Msa0622180:intron 35.0%
TTCCACCACACAAAATGAAA+CGG - chr4_2:74531716-74531735 None:intergenic 35.0%
!! TTTCATTTTGTGTGGTGGAA+TGG + chr4_2:74531716-74531735 Msa0622180:intron 35.0%
TGAAAACTGAGGAGAATTTC+AGG - chr4_2:74531982-74532001 None:intergenic 35.0%
CCACGAAAAATTGAAAACTG+AGG - chr4_2:74531993-74532012 None:intergenic 35.0%
! CCTCAGTTTTCAATTTTTCG+TGG + chr4_2:74531990-74532009 Msa0622180:intron 35.0%
ATCCTCTATGTTCAATGGTT+TGG + chr4_2:74532333-74532352 Msa0622180:CDS 35.0%
GAAATTCTGTGAGATGTGAA+AGG + chr4_2:74532383-74532402 Msa0622180:CDS 35.0%
TCTGGCACTATTAATGTTGA+TGG + chr4_2:74532466-74532485 Msa0622180:CDS 35.0%
! TAAGAGCTTGTTTCGAATTC+CGG + chr4_2:74532616-74532635 Msa0622180:intron 35.0%
TGACAAAAGCTACACTTTGA+AGG - chr4_2:74532683-74532702 None:intergenic 35.0%
GTACATGTTTGTATACACGT+TGG - chr4_2:74532732-74532751 None:intergenic 35.0%
GTTAATCATCTGCATCATTC+TGG + chr4_2:74532827-74532846 Msa0622180:intron 35.0%
!!! CATTGACTCTCTTACTCTTT+TGG - chr4_2:74532953-74532972 None:intergenic 35.0%
ATCACTTGACGATCATGTAA+AGG + chr4_2:74533023-74533042 Msa0622180:intron 35.0%
ATGCTCAAGTTCTTGATCAA+TGG - chr4_2:74533133-74533152 None:intergenic 35.0%
!! AAGTTTGACTCGTTTTACAG+TGG + chr4_2:74533159-74533178 Msa0622180:intron 35.0%
TACCTGTCTAACAATTGAGA+GGG - chr4_2:74533193-74533212 None:intergenic 35.0%
TTACCTGTCTAACAATTGAG+AGG - chr4_2:74533194-74533213 None:intergenic 35.0%
!! AATAAGTGCTGGGATAACAA+TGG + chr4_2:74533342-74533361 Msa0622180:intron 35.0%
TCTGTCCTATTTGATGGAAT+TGG + chr4_2:74533367-74533386 Msa0622180:intron 35.0%
ATCTTGATCAAATACCGATC+TGG - chr4_2:74533515-74533534 None:intergenic 35.0%
! ACGGTTTTCATAGCAAATGT+TGG + chr4_2:74533659-74533678 Msa0622180:CDS 35.0%
GAGAGAATAATTCAGTGTCA+AGG + chr4_2:74533866-74533885 Msa0622180:CDS 35.0%
ATATTCCTCTGAGTTACTTC+AGG - chr4_2:74534022-74534041 None:intergenic 35.0%
CTCTTGTAGAAATGAAACTC+TGG + chr4_2:74534188-74534207 Msa0622180:intron 35.0%
AACAATAAACTCTCACACAC+AGG + chr4_2:74534226-74534245 Msa0622180:intron 35.0%
TCACCGGTCATATTACAATT+TGG + chr4_2:74534430-74534449 Msa0622180:intron 35.0%
CTGCCAAATTGTAATATGAC+CGG - chr4_2:74534436-74534455 None:intergenic 35.0%
ATTACAATTTGGCAGTTGAC+TGG + chr4_2:74534441-74534460 Msa0622180:intron 35.0%
!! TTTAGTTTGGTTCAACCAGT+TGG - chr4_2:74534475-74534494 None:intergenic 35.0%
GGAGTTACATTAAGTACTTG+TGG + chr4_2:74534530-74534549 Msa0622180:intron 35.0%
!!! TTTGTTTTGTGATGCAGGTT+TGG + chr4_2:74534553-74534572 Msa0622180:intron 35.0%
!! TTGTTTTGTGATGCAGGTTT+GGG + chr4_2:74534554-74534573 Msa0622180:intron 35.0%
AATGTCTACTGCTTCTTGAT+TGG - chr4_2:74534589-74534608 None:intergenic 35.0%
!! TTAGCTGATTTTGCTTTGTC+TGG - chr4_2:74534624-74534643 None:intergenic 35.0%
AGCAAAATCAGCTAAACGTT+TGG + chr4_2:74534629-74534648 Msa0622180:CDS 35.0%
AAAATCAGCTAAACGTTTGG+TGG + chr4_2:74534632-74534651 Msa0622180:CDS 35.0%
TTCAGGAATTCACTGTGTAG+AGG - chr4_2:74531965-74531984 None:intergenic 40.0%
TGCCAAACCATTGAACATAG+AGG - chr4_2:74532338-74532357 None:intergenic 40.0%
! GACTTGATTTTGTGTAGCTC+TGG + chr4_2:74532448-74532467 Msa0622180:CDS 40.0%
!! ACCTCTTTTGGAGAAAACTG+AGG - chr4_2:74532506-74532525 None:intergenic 40.0%
TTCTCCAAAAGAGGTCAGAA+AGG + chr4_2:74532511-74532530 Msa0622180:CDS 40.0%
AACCAATCAAGACTGTTGCA+TGG + chr4_2:74532534-74532553 Msa0622180:CDS 40.0%
! AAGAGCTTGTTTCGAATTCC+GGG + chr4_2:74532617-74532636 Msa0622180:intron 40.0%
! CTTGATTTTGTCAAAACCCC+CGG - chr4_2:74532638-74532657 None:intergenic 40.0%
CGTTGGGTTTGACAAAATCA+CGG - chr4_2:74532715-74532734 None:intergenic 40.0%
ATAGGAATTTGGATGCCAAG+AGG + chr4_2:74532873-74532892 Msa0622180:intron 40.0%
CAAGAGGACATGATATTCTG+CGG + chr4_2:74532889-74532908 Msa0622180:CDS 40.0%
! TGCGGTATATTTGATGGACA+CGG + chr4_2:74532907-74532926 Msa0622180:CDS 40.0%
CAATTGCATAGCAAAGACCT+TGG - chr4_2:74532976-74532995 None:intergenic 40.0%
! CAAGGTCTTTGCTATGCAAT+TGG + chr4_2:74532974-74532993 Msa0622180:CDS 40.0%
AACAACTTTCGAGAATCCGT+AGG + chr4_2:74533223-74533242 Msa0622180:intron 40.0%
ACAACTTTCGAGAATCCGTA+GGG + chr4_2:74533224-74533243 Msa0622180:intron 40.0%
ATGGCATCTGTCCTATTTGA+TGG + chr4_2:74533361-74533380 Msa0622180:intron 40.0%
TGATGACATGGAATCCAGAT+CGG + chr4_2:74533498-74533517 Msa0622180:intron 40.0%
!! ATCAGACGATGATGGAAGTT+TGG + chr4_2:74533709-74533728 Msa0622180:CDS 40.0%
TTGGTTCAACCAGTTGGATT+AGG - chr4_2:74534469-74534488 None:intergenic 40.0%
!!! TGTGGTTTGTTTTGTGATGC+AGG + chr4_2:74534548-74534567 Msa0622180:intron 40.0%
TAAGCCCAACTTCATGATGT+TGG + chr4_2:74534792-74534811 Msa0622180:three_prime_UTR 40.0%
AAGCCCAACTTCATGATGTT+GGG + chr4_2:74534793-74534812 Msa0622180:three_prime_UTR 40.0%
GAACCCAACATCATGAAGTT+GGG - chr4_2:74534799-74534818 None:intergenic 40.0%
TGAACCCAACATCATGAAGT+TGG - chr4_2:74534800-74534819 None:intergenic 40.0%
TGTTGGGTTCATATGACCTT+TGG + chr4_2:74534809-74534828 Msa0622180:three_prime_UTR 40.0%
ATATGACCTTTGGTCCAGTT+AGG + chr4_2:74534819-74534838 Msa0622180:three_prime_UTR 40.0%
TATGACCTTTGGTCCAGTTA+GGG + chr4_2:74534820-74534839 Msa0622180:three_prime_UTR 40.0%
GTAACAGTTGTGCTGAGTTG+AGG - chr4_2:74531241-74531260 None:intergenic 45.0%
! CACCGTTTCATTTTGTGTGG+TGG + chr4_2:74531711-74531730 Msa0622180:intron 45.0%
GGTGGAATGGAATCCTCTTA+TGG + chr4_2:74531729-74531748 Msa0622180:intron 45.0%
AAACAGTAGCTAGCTAAGCG+TGG - chr4_2:74531860-74531879 None:intergenic 45.0%
GAGAGAAGCTGTTGAAGCAA+TGG + chr4_2:74532405-74532424 Msa0622180:CDS 45.0%
! GCCTCAGTTTTCTCCAAAAG+AGG + chr4_2:74532502-74532521 Msa0622180:CDS 45.0%
! GGTTCCTTTCTGACCTCTTT+TGG - chr4_2:74532518-74532537 None:intergenic 45.0%
CACCATGCAACAGTCTTGAT+TGG - chr4_2:74532539-74532558 None:intergenic 45.0%
ATCAAGACTGTTGCATGGTG+TGG + chr4_2:74532539-74532558 Msa0622180:CDS 45.0%
TCAAGACTGTTGCATGGTGT+GGG + chr4_2:74532540-74532559 Msa0622180:CDS 45.0%
! AGAGCTTGTTTCGAATTCCG+GGG + chr4_2:74532618-74532637 Msa0622180:intron 45.0%
!! CGGGGGTTTTGACAAAATCA+AGG + chr4_2:74532636-74532655 Msa0622180:intron 45.0%
CGCAGAATATCATGTCCTCT+TGG - chr4_2:74532891-74532910 None:intergenic 45.0%
! GCGGTATATTTGATGGACAC+GGG + chr4_2:74532908-74532927 Msa0622180:CDS 45.0%
GAGTAAGAGAGTCAATGCCA+AGG + chr4_2:74532956-74532975 Msa0622180:CDS 45.0%
TGCCCTCTCAATTGTTAGAC+AGG + chr4_2:74533188-74533207 Msa0622180:intron 45.0%
! TAATGGTTTGCAGGGGGAAA+CGG + chr4_2:74533640-74533659 Msa0622180:intron 45.0%
GCTACAACATCAGACGATGA+TGG + chr4_2:74533701-74533720 Msa0622180:CDS 45.0%
! ATGATGAACCAGGAGTGCAT+AGG + chr4_2:74533906-74533925 Msa0622180:CDS 45.0%
! TGATGAACCAGGAGTGCATA+GGG + chr4_2:74533907-74533926 Msa0622180:CDS 45.0%
TTACCAGATGTAGAGTCTCC+AGG + chr4_2:74533935-74533954 Msa0622180:CDS 45.0%
ATCTCCAAAAGCCCTTGACA+TGG - chr4_2:74533966-74533985 None:intergenic 45.0%
CAGTACCTGAAGTAACTCAG+AGG + chr4_2:74534014-74534033 Msa0622180:CDS 45.0%
! TTTGTTGTGCTTGCCACTGA+TGG + chr4_2:74534058-74534077 Msa0622180:CDS 45.0%
TTGTTGTGCTTGCCACTGAT+GGG + chr4_2:74534059-74534078 Msa0622180:CDS 45.0%
CAAGATACTTACCCCATCAG+TGG - chr4_2:74534074-74534093 None:intergenic 45.0%
! TGACTGGTTCCTAATCCAAC+TGG + chr4_2:74534457-74534476 Msa0622180:intron 45.0%
TGGAATGTGCAGTACATGCA+TGG + chr4_2:74534652-74534671 Msa0622180:CDS 45.0%
ATGCATGGAAACGCAAGAGA+AGG + chr4_2:74534667-74534686 Msa0622180:CDS 45.0%
TGCATGGAAACGCAAGAGAA+GGG + chr4_2:74534668-74534687 Msa0622180:CDS 45.0%
CTATCTTGCTCAGGGGAAAT+GGG - chr4_2:74534741-74534760 None:intergenic 45.0%
AATTGGGCTATCTTGCTCAG+GGG - chr4_2:74534748-74534767 None:intergenic 45.0%
CAATTGGGCTATCTTGCTCA+GGG - chr4_2:74534749-74534768 None:intergenic 45.0%
TCAATTGGGCTATCTTGCTC+AGG - chr4_2:74534750-74534769 None:intergenic 45.0%
TTCTACCCTAACTGGACCAA+AGG - chr4_2:74534828-74534847 None:intergenic 45.0%
!!! TTAGTTAATTTATTTTATAA+TGG + chr4_2:74533623-74533642 Msa0622180:intron 5.0%
!! TCATTTATAATTATATAAAT+TGG + chr4_2:74533803-74533822 Msa0622180:intron 5.0%
! GTTGTGCTGAGTTGAGGAAC+AGG - chr4_2:74531235-74531254 None:intergenic 50.0%
! GAGCTTGTTTCGAATTCCGG+GGG + chr4_2:74532619-74532638 Msa0622180:intron 50.0%
! TATTTGATGGACACGGGCCA+TGG + chr4_2:74532914-74532933 Msa0622180:CDS 50.0%
! ATTTGATGGACACGGGCCAT+GGG + chr4_2:74532915-74532934 Msa0622180:CDS 50.0%
TTGGCTACAAAGTGACCCCA+TGG - chr4_2:74532934-74532953 None:intergenic 50.0%
AGTCCTGGAGACTCTACATC+TGG - chr4_2:74533941-74533960 None:intergenic 50.0%
TCCAGGACTTGCCATGTCAA+GGG + chr4_2:74533952-74533971 Msa0622180:CDS 50.0%
! CTTGCCATGTCAAGGGCTTT+TGG + chr4_2:74533959-74533978 Msa0622180:CDS 50.0%
CAGTGGCAAGCACAACAAAC+TGG - chr4_2:74534057-74534076 None:intergenic 50.0%
TGTTGTGCTTGCCACTGATG+GGG + chr4_2:74534060-74534079 Msa0622180:CDS 50.0%
GCATGGAAACGCAAGAGAAG+GGG + chr4_2:74534669-74534688 Msa0622180:CDS 50.0%
GCTATCTTGCTCAGGGGAAA+TGG - chr4_2:74534742-74534761 None:intergenic 50.0%
AGACTGTTGCATGGTGTGGG+AGG + chr4_2:74532543-74532562 Msa0622180:CDS 55.0%
! TTTGATGGACACGGGCCATG+GGG + chr4_2:74532916-74532935 Msa0622180:CDS 55.0%
TGGTGACTCTCGTGCAGTAC+TGG + chr4_2:74533679-74533698 Msa0622180:CDS 55.0%
! AACCAGGAGTGCATAGGGTC+TGG + chr4_2:74533912-74533931 Msa0622180:CDS 55.0%
AACCAGACCCTATGCACTCC+TGG - chr4_2:74533917-74533936 None:intergenic 55.0%
CTCCAGGACTTGCCATGTCA+AGG + chr4_2:74533951-74533970 Msa0622180:CDS 55.0%
CTTGCTCAGGGGAAATGGGA+GGG - chr4_2:74534737-74534756 None:intergenic 55.0%
TCTTGCTCAGGGGAAATGGG+AGG - chr4_2:74534738-74534757 None:intergenic 55.0%
GCCCTTGACATGGCAAGTCC+TGG - chr4_2:74533956-74533975 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 74531102 74534901 74531102 ID=Msa0622180;Name=Msa0622180
chr4_2 mRNA 74531102 74534901 74531102 ID=Msa0622180-mRNA-1;Parent=Msa0622180;Name=Msa0622180-mRNA-1;_AED=0.19;_eAED=0.19;_QI=243|0.8|0.83|1|0.8|0.66|6|119|320
chr4_2 exon 74531102 74531284 74531102 ID=Msa0622180-mRNA-1:exon:22499;Parent=Msa0622180-mRNA-1
chr4_2 exon 74532262 74532564 74532262 ID=Msa0622180-mRNA-1:exon:22500;Parent=Msa0622180-mRNA-1
chr4_2 exon 74532877 74533038 74532877 ID=Msa0622180-mRNA-1:exon:22501;Parent=Msa0622180-mRNA-1
chr4_2 exon 74533653 74533773 74533653 ID=Msa0622180-mRNA-1:exon:22502;Parent=Msa0622180-mRNA-1
chr4_2 exon 74533858 74534081 74533858 ID=Msa0622180-mRNA-1:exon:22503;Parent=Msa0622180-mRNA-1
chr4_2 exon 74534570 74534901 74534570 ID=Msa0622180-mRNA-1:exon:22504;Parent=Msa0622180-mRNA-1
chr4_2 five_prime_UTR 74531102 74531284 74531102 ID=Msa0622180-mRNA-1:five_prime_utr;Parent=Msa0622180-mRNA-1
chr4_2 five_prime_UTR 74532262 74532321 74532262 ID=Msa0622180-mRNA-1:five_prime_utr;Parent=Msa0622180-mRNA-1
chr4_2 CDS 74532322 74532564 74532322 ID=Msa0622180-mRNA-1:cds;Parent=Msa0622180-mRNA-1
chr4_2 CDS 74532877 74533038 74532877 ID=Msa0622180-mRNA-1:cds;Parent=Msa0622180-mRNA-1
chr4_2 CDS 74533653 74533773 74533653 ID=Msa0622180-mRNA-1:cds;Parent=Msa0622180-mRNA-1
chr4_2 CDS 74533858 74534081 74533858 ID=Msa0622180-mRNA-1:cds;Parent=Msa0622180-mRNA-1
chr4_2 CDS 74534570 74534782 74534570 ID=Msa0622180-mRNA-1:cds;Parent=Msa0622180-mRNA-1
chr4_2 three_prime_UTR 74534783 74534901 74534783 ID=Msa0622180-mRNA-1:three_prime_utr;Parent=Msa0622180-mRNA-1
Gene Sequence

>Msa0622180

ATGGGGCATTTATCCTCTATGTTCAATGGTTTGGCAAGATCATTTTCACTAAAGAAAGGAAGAAATTCTGTGAGATGTGAAAGGAGAGAAGCTGTTGAAGCAATGGCAAAAGAAGCTAAGAAAAATGACTTGATTTTGTGTAGCTCTGGCACTATTAATGTTGATGGTTCAAACAACTTTGCCTCAGTTTTCTCCAAAAGAGGTCAGAAAGGAACCAATCAAGACTGTTGCATGGTGTGGGAGGAATTTGGATGCCAAGAGGACATGATATTCTGCGGTATATTTGATGGACACGGGCCATGGGGTCACTTTGTAGCCAAAAGAGTAAGAGAGTCAATGCCAAGGTCTTTGCTATGCAATTGGCAAGAGACACTTGCTACACAATCTTCATCACTTGACGATCATGGGGAAACGGTTTTCATAGCAAATGTTGGTGACTCTCGTGCAGTACTGGCTACAACATCAGACGATGATGGAAGTTTGGTAGCAGTACAACTAACAGTTGATTTCAAGCCAAATTTACCTCAAGAGGAAGAGAGAATAATTCAGTGTCAAGGGCGTGTGTTCTGTTTAAATGATGAACCAGGAGTGCATAGGGTCTGGTTACCAGATGTAGAGTCTCCAGGACTTGCCATGTCAAGGGCTTTTGGAGATTATTGTATTAAAGATTATGGTCTTATTTCAGTACCTGAAGTAACTCAGAGGAATATAAGTAGTAAAGACCAGTTTGTTGTGCTTGCCACTGATGGGGTTTGGGATGTGATCTCCAATCAAGAAGCAGTAGACATTGTATCTTCAACACCAGACAAAGCAAAATCAGCTAAACGTTTGGTGGAATGTGCAGTACATGCATGGAAACGCAAGAGAAGGGGTATTGCCATTGATGATATTTCTGCTATTTGTCTCTTTTTGCACCCTCCCATTTCCCCTGAGCAAGATAGCCCAATTGAAACTGTTAAATAG
Protein sequence

>Msa0622180

MGHLSSMFNGLARSFSLKKGRNSVRCERREAVEAMAKEAKKNDLILCSSGTINVDGSNNFASVFSKRGQKGTNQDCCMVWEEFGCQEDMIFCGIFDGHGPWGHFVAKRVRESMPRSLLCNWQETLATQSSSLDDHGETVFIANVGDSRAVLATTSDDDGSLVAVQLTVDFKPNLPQEEERIIQCQGRVFCLNDEPGVHRVWLPDVESPGLAMSRAFGDYCIKDYGLISVPEVTQRNISSKDQFVVLATDGVWDVISNQEAVDIVSSTPDKAKSAKRLVECAVHAWKRKRRGIAIDDISAICLFLHPPISPEQDSPIETVK*