AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1304880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1304880 MtrunA17_Chr7g0272061 79.310 377 21 2 1 320 1 377 0.0 598
Msa1304880 MtrunA17_Chr1g0192421 68.362 354 60 3 1 305 1 351 1.20e-166 467
Msa1304880 MtrunA17_Chr4g0067711 54.802 354 110 2 1 305 1 353 6.46e-141 402
Msa1304880 MtrunA17_Chr2g0295401 55.211 355 108 3 1 305 1 354 1.05e-139 399
Msa1304880 MtrunA17_Chr7g0243571 40.798 326 128 4 52 314 53 376 5.91e-85 263
Msa1304880 MtrunA17_Chr4g0022171 40.741 324 128 4 52 313 58 379 3.10e-83 259
Msa1304880 MtrunA17_Chr4g0004971 40.924 303 125 3 56 305 43 344 1.65e-79 246
Msa1304880 MtrunA17_Chr7g0249421 59.218 179 72 1 126 304 207 384 6.24e-66 215
Msa1304880 MtrunA17_Chr7g0249421 59.155 71 29 0 52 122 57 127 2.34e-24 103
Msa1304880 MtrunA17_Chr7g0221691 57.778 180 74 2 126 305 232 409 4.48e-64 209
Msa1304880 MtrunA17_Chr7g0221691 45.977 87 47 0 50 136 88 174 1.45e-20 91.7
Msa1304880 MtrunA17_Chr8g0342431 59.649 171 66 3 135 305 206 373 5.65e-62 202
Msa1304880 MtrunA17_Chr8g0342431 52.632 76 36 0 50 125 45 120 4.67e-22 95.5
Msa1304880 MtrunA17_Chr2g0324061 51.337 187 90 1 126 312 186 371 7.93e-60 198
Msa1304880 MtrunA17_Chr2g0324061 51.562 64 30 1 55 118 55 117 3.35e-17 82.0
Msa1304880 MtrunA17_Chr1g0151781 37.966 295 131 4 60 305 38 329 1.18e-58 192
Msa1304880 MtrunA17_Chr6g0486661 29.831 295 154 7 62 305 90 382 4.76e-30 120
Msa1304880 MtrunA17_Chr4g0066371 27.636 275 150 7 73 302 68 338 7.10e-25 103
Msa1304880 MtrunA17_Chr2g0317021 35.366 246 105 11 87 304 113 332 8.64e-23 96.7
Msa1304880 MtrunA17_Chr2g0286951 28.289 304 161 10 57 307 45 344 1.39e-22 97.4
Msa1304880 MtrunA17_Chr8g0360351 33.074 257 116 12 79 305 52 282 3.12e-22 94.4
Msa1304880 MtrunA17_Chr3g0136711 31.336 217 102 7 93 281 67 264 9.72e-22 93.2
Msa1304880 MtrunA17_Chr2g0279151 39.881 168 77 7 135 302 135 278 1.17e-21 92.8
Msa1304880 MtrunA17_Chr1g0150451 30.876 217 103 6 93 281 110 307 2.10e-21 92.8
Msa1304880 MtrunA17_Chr1g0212961 28.309 272 145 10 39 281 71 321 2.00e-20 90.5
Msa1304880 MtrunA17_Chr5g0426141 39.286 168 78 7 135 302 136 279 6.79e-20 87.8
Msa1304880 MtrunA17_Chr3g0115851 40.120 167 76 7 136 302 137 279 7.21e-20 87.8
Msa1304880 MtrunA17_Chr6g0483411 36.310 168 78 4 136 290 155 306 8.51e-20 88.2
Msa1304880 MtrunA17_Chr4g0050381 37.952 166 79 7 137 302 133 274 3.30e-19 85.9
Msa1304880 MtrunA17_Chr5g0396731 42.308 130 58 4 137 266 188 300 5.52e-19 86.3
Msa1304880 MtrunA17_Chr3g0105371 31.655 278 135 12 62 308 89 342 4.99e-18 83.6
Msa1304880 MtrunA17_Chr3g0126511 28.090 267 136 10 65 302 99 338 5.75e-18 83.6
Msa1304880 MtrunA17_Chr4g0051661 29.918 244 118 12 88 304 180 397 6.20e-18 83.6
Msa1304880 MtrunA17_Chr3g0112291 28.339 307 157 10 3 264 33 321 2.61e-17 82.0
Msa1304880 MtrunA17_Chr1g0171571 32.022 178 98 7 136 312 497 652 6.86e-16 78.2
Msa1304880 MtrunA17_Chr5g0430481 28.623 276 154 11 54 304 117 374 7.02e-16 77.4
Msa1304880 MtrunA17_Chr5g0435721 39.844 128 60 4 137 264 309 419 7.27e-16 78.2
Msa1304880 MtrunA17_Chr8g0371321 30.144 209 94 10 136 304 157 353 1.11e-15 77.0
Msa1304880 MtrunA17_Chr3g0105351 30.657 274 135 12 62 304 65 314 1.26e-15 76.3
Msa1304880 MtrunA17_Chr4g0070871 32.075 212 86 8 91 263 253 445 1.62e-15 77.0
Msa1304880 MtrunA17_Chr1g0213351 27.645 293 129 13 91 310 80 362 1.03e-14 74.3
Msa1304880 MtrunA17_Chr1g0158781 40.164 122 56 4 143 264 226 330 1.06e-14 74.3
Msa1304880 MtrunA17_Chr3g0135961 29.200 250 105 10 59 263 262 484 2.14e-14 73.6
Msa1304880 MtrunA17_Chr1g0211251 32.195 205 91 9 138 304 161 355 5.06e-14 72.0
Msa1304880 MtrunA17_Chr3g0089711 27.393 303 129 13 88 312 65 354 1.01e-13 71.2
Msa1304880 MtrunA17_Chr3g0089721 27.393 303 129 13 88 312 65 354 1.30e-13 70.9
Msa1304880 MtrunA17_Chr5g0407161 29.808 208 89 7 91 264 124 308 4.08e-13 69.3
Msa1304880 MtrunA17_Chr3g0138281 26.923 260 117 12 91 307 121 350 5.80e-13 68.9
Msa1304880 MtrunA17_Chr3g0089691 30.275 218 99 9 137 312 62 268 6.79e-13 68.2
Msa1304880 MtrunA17_Chr7g0256751 27.668 253 114 12 88 282 66 307 8.99e-13 68.6
Msa1304880 MtrunA17_Chr4g0065141 25.391 256 140 10 85 307 118 355 1.19e-12 68.2
Msa1304880 MtrunA17_Chr1g0150781 37.600 125 61 4 139 263 381 488 2.96e-12 67.4
Msa1304880 MtrunA17_Chr4g0067301 32.051 156 73 4 135 266 286 432 5.86e-12 66.2
Msa1304880 MtrunA17_Chr8g0389921 29.327 208 93 6 91 263 198 386 9.57e-12 65.5
Msa1304880 MtrunA17_Chr3g0144111 29.348 184 98 5 91 263 27 189 1.15e-11 63.9
Msa1304880 MtrunA17_Chr1g0194191 32.090 134 67 5 136 264 198 312 3.50e-11 63.5
Msa1304880 MtrunA17_Chr1g0150071 30.597 134 66 4 139 264 173 287 7.11e-11 62.4
Msa1304880 MtrunA17_Chr1g0209451 28.638 213 117 11 111 307 107 300 8.12e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1304880 AT3G05640.2 66.097 351 72 3 1 305 1 350 5.47e-167 469
Msa1304880 AT3G05640.1 66.097 351 72 3 1 305 1 350 5.47e-167 469
Msa1304880 AT3G05640.3 66.097 351 72 3 1 305 1 350 5.47e-167 469
Msa1304880 AT5G27930.1 62.994 354 79 4 1 303 1 353 8.59e-162 456
Msa1304880 AT5G27930.2 62.994 354 79 4 1 303 1 353 8.59e-162 456
Msa1304880 AT5G27930.3 62.252 302 62 4 1 251 1 301 2.39e-132 379
Msa1304880 AT3G16800.2 52.286 350 123 3 1 306 1 350 1.38e-124 361
Msa1304880 AT3G16800.1 52.286 350 123 3 1 306 1 350 1.38e-124 361
Msa1304880 AT3G16800.3 52.286 350 123 3 1 306 1 350 1.38e-124 361
Msa1304880 AT3G16800.4 54.510 255 74 2 94 306 1 255 7.69e-93 276
Msa1304880 AT3G16800.6 54.510 255 74 2 94 306 1 255 7.69e-93 276
Msa1304880 AT1G03590.1 41.290 310 120 4 55 304 55 362 1.88e-78 247
Msa1304880 AT5G01700.2 42.071 309 128 5 52 311 39 345 4.91e-76 238
Msa1304880 AT5G01700.1 43.051 295 117 5 66 311 4 296 2.08e-75 235
Msa1304880 AT4G32950.1 42.105 285 131 4 59 311 41 323 5.91e-71 223
Msa1304880 AT1G79630.6 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa1304880 AT1G79630.3 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa1304880 AT1G79630.4 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa1304880 AT1G79630.5 56.983 179 76 1 127 305 113 290 6.73e-70 223
Msa1304880 AT1G79630.2 56.667 180 77 1 126 305 152 330 6.16e-69 221
Msa1304880 AT1G79630.2 50.000 52 26 0 81 132 18 69 3.00e-11 64.3
Msa1304880 AT1G79630.7 56.667 180 77 1 126 305 152 330 6.16e-69 221
Msa1304880 AT1G79630.7 50.000 52 26 0 81 132 18 69 3.00e-11 64.3
Msa1304880 AT1G79630.1 56.667 180 77 1 126 305 222 400 1.85e-68 222
Msa1304880 AT1G79630.1 45.783 83 45 0 50 132 57 139 2.81e-20 91.7
Msa1304880 AT1G79630.8 56.667 180 77 1 126 305 218 396 1.94e-68 222
Msa1304880 AT1G79630.8 45.783 83 45 0 50 132 53 135 3.55e-20 91.3
Msa1304880 AT1G16220.1 55.556 180 79 1 126 305 199 377 2.74e-66 216
Msa1304880 AT1G16220.1 45.122 82 45 0 50 131 54 135 1.65e-19 89.4
Msa1304880 AT5G26010.1 42.123 292 128 5 58 310 41 330 2.98e-65 209
Msa1304880 AT3G02750.2 56.983 179 76 1 126 304 216 393 1.70e-63 209
Msa1304880 AT3G02750.2 39.450 109 63 1 14 122 21 126 4.36e-23 99.8
Msa1304880 AT3G02750.1 56.983 179 76 1 126 304 216 393 1.70e-63 209
Msa1304880 AT3G02750.1 39.450 109 63 1 14 122 21 126 4.36e-23 99.8
Msa1304880 AT5G36250.1 51.397 179 86 1 126 304 207 384 3.65e-61 201
Msa1304880 AT5G36250.1 44.444 108 51 2 50 148 57 164 6.32e-22 95.9
Msa1304880 AT4G03415.1 54.190 179 81 1 135 313 204 381 1.18e-60 201
Msa1304880 AT4G03415.1 47.826 69 35 1 63 131 70 137 2.83e-16 79.7
Msa1304880 AT4G03415.3 54.190 179 81 1 135 313 204 381 1.18e-60 201
Msa1304880 AT4G03415.3 47.826 69 35 1 63 131 70 137 2.83e-16 79.7
Msa1304880 AT4G03415.2 54.190 179 81 1 135 313 204 381 1.18e-60 201
Msa1304880 AT4G03415.2 47.826 69 35 1 63 131 70 137 2.83e-16 79.7
Msa1304880 AT5G36250.2 48.187 193 85 2 126 304 207 398 1.70e-57 192
Msa1304880 AT5G36250.2 44.444 108 51 2 50 148 57 164 9.04e-22 95.9
Msa1304880 AT3G02750.3 47.664 214 76 2 126 304 216 428 2.17e-56 191
Msa1304880 AT3G02750.3 37.391 115 69 1 14 128 21 132 8.80e-23 99.0
Msa1304880 AT3G16800.5 45.205 219 76 3 1 175 1 219 3.38e-56 182
Msa1304880 AT4G32950.2 43.111 225 94 4 59 251 41 263 2.75e-53 176
Msa1304880 AT5G36250.3 53.521 142 64 2 126 266 207 347 4.21e-45 157
Msa1304880 AT5G36250.3 44.037 109 52 2 50 149 57 165 4.68e-22 95.5
Msa1304880 AT2G20050.2 30.847 295 146 9 62 304 109 397 3.10e-30 121
Msa1304880 AT2G20050.1 30.847 295 146 9 62 304 109 397 3.50e-30 121
Msa1304880 AT2G20630.1 33.607 244 108 10 87 302 59 276 1.08e-24 101
Msa1304880 AT2G20630.2 33.884 242 106 10 88 301 60 275 3.33e-24 100
Msa1304880 AT3G06270.1 29.304 273 137 8 50 269 40 309 3.48e-24 101
Msa1304880 AT4G28400.3 33.198 247 111 12 87 305 41 261 5.52e-24 99.0
Msa1304880 AT4G28400.1 34.167 240 104 12 94 305 70 283 6.12e-24 99.4
Msa1304880 AT4G31750.1 31.390 223 103 8 93 287 66 266 2.36e-21 92.8
Msa1304880 AT4G31750.3 31.390 223 103 8 93 287 66 266 2.36e-21 92.8
Msa1304880 AT4G31750.2 31.390 223 103 8 93 287 66 266 2.39e-21 92.8
Msa1304880 AT5G24940.1 31.301 246 108 8 91 304 64 280 7.06e-21 93.2
Msa1304880 AT5G10740.1 31.174 247 109 8 91 305 64 281 1.12e-20 91.7
Msa1304880 AT5G10740.2 31.174 247 109 8 91 305 64 281 1.12e-20 91.7
Msa1304880 AT1G34750.2 33.333 204 98 9 115 304 102 281 6.13e-19 85.5
Msa1304880 AT1G34750.1 33.333 204 98 9 115 304 102 281 6.13e-19 85.5
Msa1304880 AT1G34750.3 33.333 204 98 9 115 304 102 281 6.13e-19 85.5
Msa1304880 AT5G59220.1 31.776 214 87 7 91 264 148 342 9.30e-19 86.7
Msa1304880 AT5G53140.1 28.221 326 177 13 9 305 52 349 1.24e-18 86.3
Msa1304880 AT5G53140.2 28.221 326 177 13 9 305 52 349 1.24e-18 86.3
Msa1304880 AT1G78200.5 31.746 252 118 12 80 302 9 235 1.72e-18 83.6
Msa1304880 AT1G78200.4 31.746 252 118 12 80 302 9 235 1.72e-18 83.6
Msa1304880 AT1G78200.1 31.746 252 118 12 80 302 54 280 2.46e-18 84.0
Msa1304880 AT1G78200.3 31.746 252 118 12 80 302 54 280 2.46e-18 84.0
Msa1304880 AT1G78200.2 31.746 252 118 12 80 302 54 280 2.46e-18 84.0
Msa1304880 AT3G15260.2 37.952 166 79 7 137 302 145 286 3.32e-18 83.6
Msa1304880 AT3G15260.1 37.952 166 79 7 137 302 145 286 3.32e-18 83.6
Msa1304880 AT1G07160.1 30.325 277 135 13 62 304 124 376 7.31e-18 84.0
Msa1304880 AT1G07430.1 40.625 128 59 4 137 264 244 354 1.29e-17 83.6
Msa1304880 AT2G40180.1 29.371 286 139 13 58 305 126 386 1.40e-17 83.2
Msa1304880 AT1G43900.1 29.508 244 122 9 65 279 128 350 1.42e-17 82.8
Msa1304880 AT3G51370.2 30.435 207 96 9 136 304 72 268 1.01e-16 79.3
Msa1304880 AT3G55050.1 31.731 208 88 10 139 305 165 359 1.04e-16 80.5
Msa1304880 AT3G55050.2 31.731 208 88 10 139 305 165 359 1.04e-16 80.5
Msa1304880 AT2G25620.1 28.462 260 131 11 91 315 128 367 1.53e-16 80.1
Msa1304880 AT2G25620.2 28.462 260 131 11 91 315 128 367 1.53e-16 80.1
Msa1304880 AT3G51370.1 30.435 207 96 8 136 304 157 353 2.34e-16 79.3
Msa1304880 AT3G51370.3 30.435 207 96 8 136 304 157 353 2.34e-16 79.3
Msa1304880 AT2G34740.1 27.652 264 138 12 67 302 94 332 6.21e-16 77.8
Msa1304880 AT3G11410.1 39.370 127 60 4 137 263 232 341 1.02e-15 77.8
Msa1304880 AT2G30020.1 28.623 276 140 12 62 304 141 392 1.52e-15 77.0
Msa1304880 AT4G26080.1 31.532 222 97 8 78 263 159 361 2.12e-15 77.0
Msa1304880 AT1G22280.1 33.929 168 87 7 135 302 135 278 3.78e-15 74.7
Msa1304880 AT1G22280.3 34.524 168 86 8 135 302 141 284 4.04e-15 74.7
Msa1304880 AT5G57050.1 31.754 211 87 8 91 263 160 351 2.96e-14 73.6
Msa1304880 AT5G57050.2 31.754 211 87 8 91 263 120 311 3.22e-14 73.2
Msa1304880 AT3G51470.2 26.115 314 153 13 50 315 12 294 5.20e-14 72.0
Msa1304880 AT5G66080.2 29.902 204 95 9 139 304 59 252 5.39e-14 71.6
Msa1304880 AT1G67820.2 29.528 254 132 10 49 275 75 308 5.73e-14 72.4
Msa1304880 AT1G67820.1 29.528 254 132 10 49 275 111 344 6.83e-14 72.4
Msa1304880 AT3G51470.1 26.115 314 153 13 50 315 56 338 8.62e-14 71.6
Msa1304880 AT5G66080.1 29.902 204 95 9 139 304 163 356 9.56e-14 71.6
Msa1304880 AT3G12620.2 31.373 204 92 9 139 304 164 357 2.12e-13 70.9
Msa1304880 AT3G12620.1 31.373 204 92 9 139 304 164 357 2.12e-13 70.9
Msa1304880 AT1G47380.1 30.137 219 120 11 112 315 104 304 3.16e-13 70.5
Msa1304880 AT1G18030.1 31.447 159 84 5 137 282 186 332 4.31e-13 69.7
Msa1304880 AT3G62260.3 30.319 188 103 8 119 300 90 255 4.62e-13 68.9
Msa1304880 AT4G28400.2 31.746 189 82 10 94 254 70 239 4.80e-13 68.6
Msa1304880 AT4G38520.2 30.244 205 93 10 139 304 162 355 4.92e-13 69.7
Msa1304880 AT4G38520.1 30.244 205 93 10 139 304 162 355 4.92e-13 69.7
Msa1304880 AT1G17550.1 37.903 124 60 3 139 262 339 445 5.08e-13 70.1
Msa1304880 AT1G17550.2 37.903 124 60 3 139 262 339 445 5.08e-13 70.1
Msa1304880 AT5G51760.2 40.000 130 58 5 134 263 212 321 5.33e-13 69.3
Msa1304880 AT5G51760.1 40.000 130 58 5 134 263 242 351 6.21e-13 69.3
Msa1304880 AT5G02760.1 28.230 209 94 8 139 306 153 346 6.24e-13 69.3
Msa1304880 AT1G48040.1 32.065 184 96 7 136 312 197 358 1.06e-12 68.6
Msa1304880 AT2G40860.1 30.857 175 98 7 139 312 504 656 1.15e-12 68.9
Msa1304880 AT3G62260.1 29.474 190 106 7 119 302 179 346 1.20e-12 68.6
Msa1304880 AT2G40860.4 30.857 175 98 7 139 312 406 558 1.23e-12 68.9
Msa1304880 AT3G62260.2 29.474 190 106 7 119 302 180 347 1.26e-12 68.6
Msa1304880 AT2G33700.1 27.106 273 127 11 39 263 58 306 1.99e-12 67.8
Msa1304880 AT1G72770.4 39.850 133 47 6 139 263 339 446 2.47e-12 67.8
Msa1304880 AT1G72770.1 39.850 133 47 6 139 263 339 446 2.47e-12 67.8
Msa1304880 AT1G72770.3 39.850 133 47 6 139 263 339 446 2.47e-12 67.8
Msa1304880 AT2G34740.2 26.271 236 127 11 67 273 108 325 4.11e-12 66.6
Msa1304880 AT3G17250.1 24.074 270 135 7 89 312 156 401 4.70e-12 66.6
Msa1304880 AT2G34740.3 26.271 236 127 11 67 273 90 307 4.88e-12 66.2
Msa1304880 AT5G06750.3 25.532 282 140 11 90 304 81 359 6.04e-12 66.2
Msa1304880 AT5G06750.1 25.532 282 140 11 90 304 81 359 6.04e-12 66.2
Msa1304880 AT1G18030.2 33.071 127 64 4 137 254 186 300 6.67e-12 65.9
Msa1304880 AT5G06750.2 28.141 199 110 7 138 304 80 277 1.94e-11 64.3
Msa1304880 AT1G68410.4 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa1304880 AT1G68410.3 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa1304880 AT1G68410.1 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa1304880 AT1G68410.2 28.767 219 123 10 112 315 113 313 2.21e-11 64.7
Msa1304880 AT1G09160.2 29.268 205 94 10 93 260 70 260 3.02e-11 64.3
Msa1304880 AT1G09160.1 29.268 205 94 10 93 260 70 260 3.02e-11 64.3
Msa1304880 AT1G34750.4 32.099 162 79 8 107 254 94 238 8.83e-11 61.6
Msa1304880 AT2G25070.1 30.597 134 66 4 139 264 173 287 9.98e-11 62.4
Msa1304880 AT2G25070.2 30.597 134 66 4 139 264 173 287 9.98e-11 62.4

Find 91 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGATTAAATTATAGGAATT+TGG 0.150752 8_3:-68161643 Msa1304880:intron
GATTATTGTATTAAAGATTA+TGG 0.199525 8_3:-68160522 Msa1304880:CDS
GGTTCCTTTCTGACCTCTTT+TGG 0.202885 8_3:+68161991 None:intergenic
CTTGCCATGTCAAGGGCTTT+TGG 0.208056 8_3:-68160546 Msa1304880:CDS
CATTGACTCTCTTACTCTTT+TGG 0.217341 8_3:+68161555 None:intergenic
AAGTACTAACGAGGTAAATT+TGG 0.252923 8_3:+68160744 None:intergenic
ATCCTCTATGTTCAATGGTT+TGG 0.312680 8_3:-68162173 Msa1304880:CDS
CGAGAAAGAAAAGATGTAAA+AGG 0.318796 8_3:+68163327 None:intergenic
GTCTATTTAACAGTTTCAAT+TGG 0.327269 8_3:+68159743 None:intergenic
AATGTCTACTGCTTCTTGAT+TGG 0.329653 8_3:+68159919 None:intergenic
TCTGGCACTATTAATGTTGA+TGG 0.338334 8_3:-68162040 Msa1304880:CDS
GCTATCTTGCTCAGGGGAAA+TGG 0.343840 8_3:+68159766 None:intergenic
TCTCAGATTCACCTTCATTA+AGG 0.358244 8_3:-68163243 Msa1304880:intron
CATTTATCCTCTATGTTCAA+TGG 0.363846 8_3:-68162178 Msa1304880:CDS
ATATTCTGCGGTATATTTGA+TGG 0.364778 8_3:-68161604 Msa1304880:CDS
ATATTCCTCTGAGTTACTTC+AGG 0.378333 8_3:+68160486 None:intergenic
ATCAGACGATGATGGAAGTT+TGG 0.389577 8_3:-68160796 Msa1304880:CDS
TCACAAAATTGAGGATTTGA+TGG 0.399038 8_3:-68162203 Msa1304880:exon
CAAGGTCTTTGCTATGCAAT+TGG 0.407044 8_3:-68161531 Msa1304880:CDS
TTTGTTGTGCTTGCCACTGA+TGG 0.408199 8_3:-68160447 Msa1304880:CDS
TCCGTAGGCCATAAATAAAT+AGG 0.409749 8_3:-68163416 None:intergenic
CACCATGCAACAGTCTTGAT+TGG 0.411872 8_3:+68161970 None:intergenic
TCAATTGGGCTATCTTGCTC+AGG 0.413365 8_3:+68159758 None:intergenic
TATTTGATGGACACGGGCCA+TGG 0.420048 8_3:-68161591 Msa1304880:CDS
CTATCTTGCTCAGGGGAAAT+GGG 0.431506 8_3:+68159767 None:intergenic
ATATGACCTTTGGTCCAGTT+AGG 0.431557 8_3:-68159686 Msa1304880:three_prime_UTR
TGTTGGGTTCATATGACCTT+TGG 0.438991 8_3:-68159696 Msa1304880:three_prime_UTR
TCTATTTAACAGTTTCAATT+GGG 0.444557 8_3:+68159744 None:intergenic
GCCCTTGACATGGCAAGTCC+TGG 0.445151 8_3:+68160552 None:intergenic
AGCAAAATCAGCTAAACGTT+TGG 0.451570 8_3:-68159876 Msa1304880:CDS
TGGTGACTCTCGTGCAGTAC+TGG 0.463725 8_3:-68160826 Msa1304880:CDS
TGAACCCAACATCATGAAGT+TGG 0.468936 8_3:+68159708 None:intergenic
AAGCCCAACTTCATGATGTT+GGG 0.471507 8_3:-68159712 Msa1304880:three_prime_UTR
ATAAATAAATAGGATCAAAC+AGG 0.472624 8_3:-68163406 Msa1304880:five_prime_UTR
TTGTTGTGCTTGCCACTGAT+GGG 0.475803 8_3:-68160446 Msa1304880:CDS
GTTGTGCTGAGTTGAGGAAC+AGG 0.476515 8_3:+68163274 None:intergenic
TATAACATAGACACATTAAG+AGG 0.479566 8_3:-68162234 Msa1304880:five_prime_UTR
CAGTGGCAAGCACAACAAAC+TGG 0.480296 8_3:+68160451 None:intergenic
TAAGCCCAACTTCATGATGT+TGG 0.481030 8_3:-68159713 Msa1304880:three_prime_UTR
AACCAGGAGTGCATAGGGTC+TGG 0.482895 8_3:-68160593 Msa1304880:CDS
CACAAAATTGAGGATTTGAT+GGG 0.483155 8_3:-68162202 Msa1304880:exon
AACCAGACCCTATGCACTCC+TGG 0.483257 8_3:+68160591 None:intergenic
CGCAGAATATCATGTCCTCT+TGG 0.484795 8_3:+68161617 None:intergenic
ATTTGATGGACACGGGCCAT+GGG 0.489246 8_3:-68161590 Msa1304880:CDS
AATAGCAGAAATATCATCAA+TGG 0.495485 8_3:+68159808 None:intergenic
TATGACCTTTGGTCCAGTTA+GGG 0.496034 8_3:-68159685 Msa1304880:three_prime_UTR
CTCCAGGACTTGCCATGTCA+AGG 0.496930 8_3:-68160554 Msa1304880:CDS
TTCTCCAAAAGAGGTCAGAA+AGG 0.500364 8_3:-68161995 Msa1304880:CDS
AGTCCTGGAGACTCTACATC+TGG 0.508363 8_3:+68160567 None:intergenic
TGATGAACCAGGAGTGCATA+GGG 0.509549 8_3:-68160598 Msa1304880:CDS
GAACCCAACATCATGAAGTT+GGG 0.509814 8_3:+68159709 None:intergenic
TTACCAGATGTAGAGTCTCC+AGG 0.526827 8_3:-68160570 Msa1304880:CDS
TGCATGGAAACGCAAGAGAA+GGG 0.527483 8_3:-68159837 Msa1304880:CDS
GAGAGAATAATTCAGTGTCA+AGG 0.530299 8_3:-68160639 Msa1304880:CDS
ATCAAGACTGTTGCATGGTG+TGG 0.533904 8_3:-68161967 Msa1304880:CDS
CAATTGGGCTATCTTGCTCA+GGG 0.538157 8_3:+68159759 None:intergenic
GAAAGTATATCACAAAATTG+AGG 0.554475 8_3:-68162212 Msa1304880:five_prime_UTR
GAGAGAAGCTGTTGAAGCAA+TGG 0.555010 8_3:-68162101 Msa1304880:CDS
TGCGGTATATTTGATGGACA+CGG 0.560385 8_3:-68161598 Msa1304880:CDS
TCCAGGACTTGCCATGTCAA+GGG 0.561968 8_3:-68160553 Msa1304880:CDS
GCTACAACATCAGACGATGA+TGG 0.562423 8_3:-68160804 Msa1304880:CDS
ATGCATGGAAACGCAAGAGA+AGG 0.563663 8_3:-68159838 Msa1304880:CDS
AAAATCAGCTAAACGTTTGG+TGG 0.569397 8_3:-68159873 Msa1304880:CDS
TGCCAAACCATTGAACATAG+AGG 0.573400 8_3:+68162171 None:intergenic
CAATTGCATAGCAAAGACCT+TGG 0.578108 8_3:+68161532 None:intergenic
GAAATTCTGTGAGATGTGAA+AGG 0.584077 8_3:-68162123 Msa1304880:CDS
GCGGTATATTTGATGGACAC+GGG 0.590895 8_3:-68161597 Msa1304880:CDS
CTTGCTCAGGGGAAATGGGA+GGG 0.592417 8_3:+68159771 None:intergenic
GTAACAGTTGTGCTGAGTTG+AGG 0.593859 8_3:+68163268 None:intergenic
CAATTAAGAGACCTTAATGA+AGG 0.594485 8_3:+68163232 None:intergenic
AGAGAATAATTCAGTGTCAA+GGG 0.594886 8_3:-68160638 Msa1304880:CDS
TCTTGCTCAGGGGAAATGGG+AGG 0.601493 8_3:+68159770 None:intergenic
TGTTGTGCTTGCCACTGATG+GGG 0.603053 8_3:-68160445 Msa1304880:intron
TTCTACCCTAACTGGACCAA+AGG 0.605758 8_3:+68159680 None:intergenic
TTGGCTACAAAGTGACCCCA+TGG 0.606377 8_3:+68161574 None:intergenic
GAGTAAGAGAGTCAATGCCA+AGG 0.613440 8_3:-68161549 Msa1304880:CDS
ATGATGAACCAGGAGTGCAT+AGG 0.615902 8_3:-68160599 Msa1304880:CDS
ATCTCCAAAAGCCCTTGACA+TGG 0.615912 8_3:+68160542 None:intergenic
TCAAGACTGTTGCATGGTGT+GGG 0.621717 8_3:-68161966 Msa1304880:CDS
TGGAATGTGCAGTACATGCA+TGG 0.626814 8_3:-68159853 Msa1304880:CDS
AACCAATCAAGACTGTTGCA+TGG 0.651730 8_3:-68161972 Msa1304880:CDS
GCATGGAAACGCAAGAGAAG+GGG 0.652422 8_3:-68159836 Msa1304880:CDS
ACAAAATTGAGGATTTGATG+GGG 0.663664 8_3:-68162201 Msa1304880:exon
TTCTGTTTAAATGATGAACC+AGG 0.665974 8_3:-68160609 Msa1304880:CDS
CAAGATACTTACCCCATCAG+TGG 0.666268 8_3:+68160434 None:intergenic
CAAGAGGACATGATATTCTG+CGG 0.676913 8_3:-68161616 Msa1304880:CDS
ATAGGAATTTGGATGCCAAG+AGG 0.678718 8_3:-68161632 Msa1304880:intron
TTTGATGGACACGGGCCATG+GGG 0.686895 8_3:-68161589 Msa1304880:CDS
AGACTGTTGCATGGTGTGGG+AGG 0.695512 8_3:-68161963 Msa1304880:intron
AATTGGGCTATCTTGCTCAG+GGG 0.712499 8_3:+68159760 None:intergenic
CAGTACCTGAAGTAACTCAG+AGG 0.714319 8_3:-68160491 Msa1304880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAAATATAACAAAA+TGG - chr8_3:68159850-68159869 Msa1304880:CDS 10.0%
!! ACGAATAAAAATTATTAATA+TGG - chr8_3:68160670-68160689 Msa1304880:intron 10.0%
!! AAAAATAAAGTCTTCATAAA+TGG - chr8_3:68159669-68159688 Msa1304880:three_prime_UTR 15.0%
!! AAAAGAAGAAAAATAATGAA+TGG + chr8_3:68159829-68159848 None:intergenic 15.0%
!!! GTATTTTTCCTATTTTTATT+TGG - chr8_3:68159872-68159891 Msa1304880:CDS 15.0%
!!! AAATATGTCACTTTTTTTTA+AGG - chr8_3:68160036-68160055 Msa1304880:intron 15.0%
!! CAAATTTATATAGAAGAAAA+AGG + chr8_3:68160734-68160753 None:intergenic 15.0%
!! ATATCAAAGACAAATAAAAT+GGG + chr8_3:68161315-68161334 None:intergenic 15.0%
!! TATATCAAAGACAAATAAAA+TGG + chr8_3:68161316-68161335 None:intergenic 15.0%
!! TTTGATATAAAATTCAATTC+TGG - chr8_3:68161327-68161346 Msa1304880:intron 15.0%
!! GATTATTGTATTAAAGATTA+TGG - chr8_3:68162508-68162527 Msa1304880:intron 15.0%
!! AAAATTGAGATTAAATATGT+TGG - chr8_3:68162779-68162798 Msa1304880:intron 15.0%
!!! TTATCTATTTATTTGTTATC+TGG + chr8_3:68162879-68162898 None:intergenic 15.0%
!! ACAAATAAATAAATAATCAC+CGG - chr8_3:68162939-68162958 Msa1304880:intron 15.0%
!!! AGTTAATTATGTTTTTAGTT+TGG + chr8_3:68163013-68163032 None:intergenic 15.0%
!! TACAAACACCAAATAAAAAT+AGG + chr8_3:68159883-68159902 None:intergenic 20.0%
!!! ATATGATTTTTGATTTTCTC+AGG - chr8_3:68160095-68160114 Msa1304880:intron 20.0%
!! TATATATTAAGTTCCATAAG+AGG + chr8_3:68160269-68160288 None:intergenic 20.0%
!! TTGAATATACTATATGCAAA+TGG + chr8_3:68160452-68160471 None:intergenic 20.0%
!! TGCATATTGAAAATAGATTA+AGG + chr8_3:68160763-68160782 None:intergenic 20.0%
!!! TGGGTTGATTATAAATATAT+AGG + chr8_3:68161296-68161315 None:intergenic 20.0%
!! TAATTTAATCGTCAATAGAT+AGG + chr8_3:68161379-68161398 None:intergenic 20.0%
!! TCTATTGACGATTAAATTAT+AGG - chr8_3:68161380-68161399 Msa1304880:intron 20.0%
!! ACGATTAAATTATAGGAATT+TGG - chr8_3:68161387-68161406 Msa1304880:intron 20.0%
!! AACAACAAAGATTCAATATA+TGG - chr8_3:68161604-68161623 Msa1304880:CDS 20.0%
!!! TTTATTTTATAATGGTTTGC+AGG - chr8_3:68162156-68162175 Msa1304880:CDS 20.0%
!!! TTATTTTATAATGGTTTGCA+GGG - chr8_3:68162157-68162176 Msa1304880:CDS 20.0%
!!! AATCATAGTAACTAGTTTAT+AGG + chr8_3:68162623-68162642 None:intergenic 20.0%
!! TTAAATTTATTGCTGAAATG+TGG - chr8_3:68163034-68163053 Msa1304880:intron 20.0%
!! TCTATTTAACAGTTTCAATT+GGG + chr8_3:68163289-68163308 None:intergenic 20.0%
!!! ATGAAGACTTTATTTTTGTC+TGG + chr8_3:68159666-68159685 None:intergenic 25.0%
! CTTACTTTGATGAATTTAAG+AGG - chr8_3:68159957-68159976 Msa1304880:intron 25.0%
!! TTTTCATGATACTGAATCAT+AGG - chr8_3:68160149-68160168 Msa1304880:intron 25.0%
! ATGGATAAAGAAATCAGAAA+AGG + chr8_3:68160417-68160436 None:intergenic 25.0%
! TATAACATAGACACATTAAG+AGG - chr8_3:68160796-68160815 Msa1304880:CDS 25.0%
!! GAAAGTATATCACAAAATTG+AGG - chr8_3:68160818-68160837 Msa1304880:CDS 25.0%
!! AGATCATTTTCACTAAAGAA+AGG - chr8_3:68160882-68160901 Msa1304880:intron 25.0%
! GATATAAAATTCAATTCTGG+TGG - chr8_3:68161330-68161349 Msa1304880:intron 25.0%
!! TTTTAATGAATTTCTCCCTA+CGG + chr8_3:68161767-68161786 None:intergenic 25.0%
! TCTGCTAACTAGTATATATA+CGG + chr8_3:68161798-68161817 None:intergenic 25.0%
! ATTTGTTCATGTTACAAGTT+TGG + chr8_3:68162003-68162022 None:intergenic 25.0%
!!! TATTTTATAATGGTTTGCAG+GGG - chr8_3:68162158-68162177 Msa1304880:CDS 25.0%
!!! AATAAATAGAAGTACTAACG+AGG + chr8_3:68162298-68162317 None:intergenic 25.0%
!! GAGTTTATTGTTGTCAATTT+TGG + chr8_3:68162743-68162762 None:intergenic 25.0%
! TGAGACATTTAGATTATGAT+TGG - chr8_3:68162837-68162856 Msa1304880:intron 25.0%
! AATAGCAGAAATATCATCAA+TGG + chr8_3:68163225-68163244 None:intergenic 25.0%
! GTCTATTTAACAGTTTCAAT+TGG + chr8_3:68163290-68163309 None:intergenic 25.0%
CGAGAAAGAAAAGATGTAAA+AGG + chr8_3:68159706-68159725 None:intergenic 30.0%
CAATTAAGAGACCTTAATGA+AGG + chr8_3:68159801-68159820 None:intergenic 30.0%
TTAACACAGTAGTTCAGTAA+TGG - chr8_3:68160358-68160377 Msa1304880:intron 30.0%
ATAAAGAAATCAGAAAAGGC+TGG + chr8_3:68160413-68160432 None:intergenic 30.0%
CAAATGGATCAAACTGAATA+TGG + chr8_3:68160436-68160455 None:intergenic 30.0%
ATTGTCTTCATGTTAAGTCA+CGG - chr8_3:68160601-68160620 Msa1304880:CDS 30.0%
TTCTTCAATAGCACATAGAA+AGG + chr8_3:68160698-68160717 None:intergenic 30.0%
TATATAGAAGAAAAAGGAGC+AGG + chr8_3:68160728-68160747 None:intergenic 30.0%
TCACAAAATTGAGGATTTGA+TGG - chr8_3:68160827-68160846 Msa1304880:CDS 30.0%
CACAAAATTGAGGATTTGAT+GGG - chr8_3:68160828-68160847 Msa1304880:CDS 30.0%
ACAAAATTGAGGATTTGATG+GGG - chr8_3:68160829-68160848 Msa1304880:CDS 30.0%
CATTTATCCTCTATGTTCAA+TGG - chr8_3:68160852-68160871 Msa1304880:CDS 30.0%
TTGAAGGTTAAACAAACTCA+CGG + chr8_3:68161192-68161211 None:intergenic 30.0%
TACATGTTTGTATACACGTT+GGG + chr8_3:68161256-68161275 None:intergenic 30.0%
! ACATGTACTCGTTACATTTT+TGG - chr8_3:68161269-68161288 Msa1304880:intron 30.0%
ATATTCTGCGGTATATTTGA+TGG - chr8_3:68161426-68161445 Msa1304880:intron 30.0%
AACAATATAGTTGCGATGTT+AGG - chr8_3:68161827-68161846 Msa1304880:intron 30.0%
ACAATATAGTTGCGATGTTA+GGG - chr8_3:68161828-68161847 Msa1304880:intron 30.0%
!! TATGTCTTTGAAATAAGTGC+TGG - chr8_3:68161856-68161875 Msa1304880:intron 30.0%
!! ATGTCTTTGAAATAAGTGCT+GGG - chr8_3:68161857-68161876 Msa1304880:intron 30.0%
GTAATCCAATTCCATCAAAT+AGG + chr8_3:68161900-68161919 None:intergenic 30.0%
GTTACAAGTTTGGTAAATGT+AGG + chr8_3:68161993-68162012 None:intergenic 30.0%
! ACATGAACAAATTGATGACA+TGG - chr8_3:68162011-68162030 Msa1304880:CDS 30.0%
AACTTCACTGATATATTGGT+TGG + chr8_3:68162109-68162128 None:intergenic 30.0%
CAAGAACTTCACTGATATAT+TGG + chr8_3:68162113-68162132 None:intergenic 30.0%
!! ATTTTATAATGGTTTGCAGG+GGG - chr8_3:68162159-68162178 Msa1304880:CDS 30.0%
AAGTACTAACGAGGTAAATT+TGG + chr8_3:68162289-68162308 None:intergenic 30.0%
TAATTGCATAATTGCAGAAG+AGG - chr8_3:68162366-68162385 Msa1304880:intron 30.0%
AGAGAATAATTCAGTGTCAA+GGG - chr8_3:68162392-68162411 Msa1304880:intron 30.0%
TTCTGTTTAAATGATGAACC+AGG - chr8_3:68162421-68162440 Msa1304880:intron 30.0%
! TTTTATCATTCTACCCTAAC+TGG + chr8_3:68163361-68163380 None:intergenic 30.0%
TCTCAGATTCACCTTCATTA+AGG - chr8_3:68159787-68159806 Msa1304880:CDS 35.0%
!! CGGTGTATTTGTTTCTGAAT+TGG + chr8_3:68160220-68160239 None:intergenic 35.0%
! ATACACCGTTTCATTTTGTG+TGG - chr8_3:68160232-68160251 Msa1304880:intron 35.0%
TTCCACCACACAAAATGAAA+CGG + chr8_3:68160240-68160259 None:intergenic 35.0%
!! TTTCATTTTGTGTGGTGGAA+TGG - chr8_3:68160240-68160259 Msa1304880:intron 35.0%
TGAAAACTGAGGAGAATTTC+AGG + chr8_3:68160506-68160525 None:intergenic 35.0%
CCACGAAAAATTGAAAACTG+AGG + chr8_3:68160517-68160536 None:intergenic 35.0%
! CCTCAGTTTTCAATTTTTCG+TGG - chr8_3:68160514-68160533 Msa1304880:CDS 35.0%
ATCCTCTATGTTCAATGGTT+TGG - chr8_3:68160857-68160876 Msa1304880:intron 35.0%
GAAATTCTGTGAGATGTGAA+AGG - chr8_3:68160907-68160926 Msa1304880:intron 35.0%
TCTGGCACTATTAATGTTGA+TGG - chr8_3:68160990-68161009 Msa1304880:intron 35.0%
! TAAGAGCTTGTTTCGAATTC+CGG - chr8_3:68161141-68161160 Msa1304880:intron 35.0%
TGACAAAAGCTACACTTTGA+AGG + chr8_3:68161208-68161227 None:intergenic 35.0%
GTACATGTTTGTATACACGT+TGG + chr8_3:68161257-68161276 None:intergenic 35.0%
GTTAATCATCTGCATCATTC+TGG - chr8_3:68161352-68161371 Msa1304880:intron 35.0%
!!! CATTGACTCTCTTACTCTTT+TGG + chr8_3:68161478-68161497 None:intergenic 35.0%
ATCACTTGACGATCATGTAA+AGG - chr8_3:68161548-68161567 Msa1304880:CDS 35.0%
ATGCTCAAGTTCTTGATCAA+TGG + chr8_3:68161658-68161677 None:intergenic 35.0%
!! AAGTTTGACTCGTTTTACAG+TGG - chr8_3:68161684-68161703 Msa1304880:intron 35.0%
TACCTGTCTAACAATTGAGA+GGG + chr8_3:68161718-68161737 None:intergenic 35.0%
TTACCTGTCTAACAATTGAG+AGG + chr8_3:68161719-68161738 None:intergenic 35.0%
!! AATAAGTGCTGGGATAACAA+TGG - chr8_3:68161867-68161886 Msa1304880:intron 35.0%
TCTGTCCTATTTGATGGAAT+TGG - chr8_3:68161892-68161911 Msa1304880:intron 35.0%
ATCTTGATCAAATACCGATC+TGG + chr8_3:68162040-68162059 None:intergenic 35.0%
! ACGGTTTTCATAGCAAATGT+TGG - chr8_3:68162184-68162203 Msa1304880:CDS 35.0%
GAGAGAATAATTCAGTGTCA+AGG - chr8_3:68162391-68162410 Msa1304880:intron 35.0%
ATATTCCTCTGAGTTACTTC+AGG + chr8_3:68162547-68162566 None:intergenic 35.0%
CTCTTGTAGAAATGAAACTC+TGG - chr8_3:68162713-68162732 Msa1304880:intron 35.0%
AACAATAAACTCTCACACAC+AGG - chr8_3:68162751-68162770 Msa1304880:intron 35.0%
TCACCGGTCATATTACAATT+TGG - chr8_3:68162955-68162974 Msa1304880:intron 35.0%
CTGCCAAATTGTAATATGAC+CGG + chr8_3:68162961-68162980 None:intergenic 35.0%
ATTACAATTTGGCAGTTGAC+TGG - chr8_3:68162966-68162985 Msa1304880:intron 35.0%
!! TTTAGTTTGGTTCAACCAGT+TGG + chr8_3:68163000-68163019 None:intergenic 35.0%
GGAGTTACATTAAGTACTTG+TGG - chr8_3:68163055-68163074 Msa1304880:intron 35.0%
!!! TTTGTTTTGTGATGCAGGTT+TGG - chr8_3:68163078-68163097 Msa1304880:intron 35.0%
!! TTGTTTTGTGATGCAGGTTT+GGG - chr8_3:68163079-68163098 Msa1304880:intron 35.0%
AATGTCTACTGCTTCTTGAT+TGG + chr8_3:68163114-68163133 None:intergenic 35.0%
!! TTAGCTGATTTTGCTTTGTC+TGG + chr8_3:68163149-68163168 None:intergenic 35.0%
AGCAAAATCAGCTAAACGTT+TGG - chr8_3:68163154-68163173 Msa1304880:intron 35.0%
AAAATCAGCTAAACGTTTGG+TGG - chr8_3:68163157-68163176 Msa1304880:intron 35.0%
TTCAGGAATTCACTGTGTAG+AGG + chr8_3:68160489-68160508 None:intergenic 40.0%
TGCCAAACCATTGAACATAG+AGG + chr8_3:68160862-68160881 None:intergenic 40.0%
! GACTTGATTTTGTGTAGCTC+TGG - chr8_3:68160972-68160991 Msa1304880:intron 40.0%
!! ACCTCTTTTGGAGAAAACTG+AGG + chr8_3:68161030-68161049 None:intergenic 40.0%
TTCTCCAAAAGAGGTCAGAA+AGG - chr8_3:68161035-68161054 Msa1304880:intron 40.0%
AACCAATCAAGACTGTTGCA+TGG - chr8_3:68161058-68161077 Msa1304880:intron 40.0%
! AAGAGCTTGTTTCGAATTCC+GGG - chr8_3:68161142-68161161 Msa1304880:intron 40.0%
! CTTGATTTTGTCAAAACCCC+CGG + chr8_3:68161163-68161182 None:intergenic 40.0%
CGTTGGGTTTGACAAAATCA+CGG + chr8_3:68161240-68161259 None:intergenic 40.0%
ATAGGAATTTGGATGCCAAG+AGG - chr8_3:68161398-68161417 Msa1304880:intron 40.0%
CAAGAGGACATGATATTCTG+CGG - chr8_3:68161414-68161433 Msa1304880:intron 40.0%
! TGCGGTATATTTGATGGACA+CGG - chr8_3:68161432-68161451 Msa1304880:intron 40.0%
CAATTGCATAGCAAAGACCT+TGG + chr8_3:68161501-68161520 None:intergenic 40.0%
! CAAGGTCTTTGCTATGCAAT+TGG - chr8_3:68161499-68161518 Msa1304880:CDS 40.0%
AACAACTTTCGAGAATCCGT+AGG - chr8_3:68161748-68161767 Msa1304880:intron 40.0%
ACAACTTTCGAGAATCCGTA+GGG - chr8_3:68161749-68161768 Msa1304880:intron 40.0%
ATGGCATCTGTCCTATTTGA+TGG - chr8_3:68161886-68161905 Msa1304880:intron 40.0%
TGATGACATGGAATCCAGAT+CGG - chr8_3:68162023-68162042 Msa1304880:CDS 40.0%
!! ATCAGACGATGATGGAAGTT+TGG - chr8_3:68162234-68162253 Msa1304880:five_prime_UTR 40.0%
TTGGTTCAACCAGTTGGATT+AGG + chr8_3:68162994-68163013 None:intergenic 40.0%
!!! TGTGGTTTGTTTTGTGATGC+AGG - chr8_3:68163073-68163092 Msa1304880:intron 40.0%
TAAGCCCAACTTCATGATGT+TGG - chr8_3:68163317-68163336 Msa1304880:five_prime_UTR 40.0%
AAGCCCAACTTCATGATGTT+GGG - chr8_3:68163318-68163337 Msa1304880:five_prime_UTR 40.0%
GAACCCAACATCATGAAGTT+GGG + chr8_3:68163324-68163343 None:intergenic 40.0%
TGAACCCAACATCATGAAGT+TGG + chr8_3:68163325-68163344 None:intergenic 40.0%
TGTTGGGTTCATATGACCTT+TGG - chr8_3:68163334-68163353 Msa1304880:five_prime_UTR 40.0%
ATATGACCTTTGGTCCAGTT+AGG - chr8_3:68163344-68163363 Msa1304880:five_prime_UTR 40.0%
TATGACCTTTGGTCCAGTTA+GGG - chr8_3:68163345-68163364 Msa1304880:five_prime_UTR 40.0%
GTAACAGTTGTGCTGAGTTG+AGG + chr8_3:68159765-68159784 None:intergenic 45.0%
! CACCGTTTCATTTTGTGTGG+TGG - chr8_3:68160235-68160254 Msa1304880:intron 45.0%
GGTGGAATGGAATCCTCTTA+TGG - chr8_3:68160253-68160272 Msa1304880:intron 45.0%
AAACAGTAGCTAGCTAAGCG+TGG + chr8_3:68160384-68160403 None:intergenic 45.0%
GAGAGAAGCTGTTGAAGCAA+TGG - chr8_3:68160929-68160948 Msa1304880:intron 45.0%
! GCCTCAGTTTTCTCCAAAAG+AGG - chr8_3:68161026-68161045 Msa1304880:intron 45.0%
! GGTTCCTTTCTGACCTCTTT+TGG + chr8_3:68161042-68161061 None:intergenic 45.0%
CACCATGCAACAGTCTTGAT+TGG + chr8_3:68161063-68161082 None:intergenic 45.0%
ATCAAGACTGTTGCATGGTG+TGG - chr8_3:68161063-68161082 Msa1304880:intron 45.0%
TCAAGACTGTTGCATGGTGT+GGG - chr8_3:68161064-68161083 Msa1304880:intron 45.0%
! AGAGCTTGTTTCGAATTCCG+GGG - chr8_3:68161143-68161162 Msa1304880:intron 45.0%
!! CGGGGGTTTTGACAAAATCA+AGG - chr8_3:68161161-68161180 Msa1304880:intron 45.0%
CGCAGAATATCATGTCCTCT+TGG + chr8_3:68161416-68161435 None:intergenic 45.0%
! GCGGTATATTTGATGGACAC+GGG - chr8_3:68161433-68161452 Msa1304880:intron 45.0%
GAGTAAGAGAGTCAATGCCA+AGG - chr8_3:68161481-68161500 Msa1304880:intron 45.0%
TGCCCTCTCAATTGTTAGAC+AGG - chr8_3:68161713-68161732 Msa1304880:intron 45.0%
! TAATGGTTTGCAGGGGGAAA+CGG - chr8_3:68162165-68162184 Msa1304880:CDS 45.0%
GCTACAACATCAGACGATGA+TGG - chr8_3:68162226-68162245 Msa1304880:five_prime_UTR 45.0%
! ATGATGAACCAGGAGTGCAT+AGG - chr8_3:68162431-68162450 Msa1304880:intron 45.0%
! TGATGAACCAGGAGTGCATA+GGG - chr8_3:68162432-68162451 Msa1304880:intron 45.0%
TTACCAGATGTAGAGTCTCC+AGG - chr8_3:68162460-68162479 Msa1304880:intron 45.0%
ATCTCCAAAAGCCCTTGACA+TGG + chr8_3:68162491-68162510 None:intergenic 45.0%
CAGTACCTGAAGTAACTCAG+AGG - chr8_3:68162539-68162558 Msa1304880:intron 45.0%
! TTTGTTGTGCTTGCCACTGA+TGG - chr8_3:68162583-68162602 Msa1304880:intron 45.0%
TTGTTGTGCTTGCCACTGAT+GGG - chr8_3:68162584-68162603 Msa1304880:intron 45.0%
CAAGATACTTACCCCATCAG+TGG + chr8_3:68162599-68162618 None:intergenic 45.0%
! TGACTGGTTCCTAATCCAAC+TGG - chr8_3:68162982-68163001 Msa1304880:intron 45.0%
TGGAATGTGCAGTACATGCA+TGG - chr8_3:68163177-68163196 Msa1304880:intron 45.0%
ATGCATGGAAACGCAAGAGA+AGG - chr8_3:68163192-68163211 Msa1304880:intron 45.0%
TGCATGGAAACGCAAGAGAA+GGG - chr8_3:68163193-68163212 Msa1304880:intron 45.0%
CTATCTTGCTCAGGGGAAAT+GGG + chr8_3:68163266-68163285 None:intergenic 45.0%
AATTGGGCTATCTTGCTCAG+GGG + chr8_3:68163273-68163292 None:intergenic 45.0%
CAATTGGGCTATCTTGCTCA+GGG + chr8_3:68163274-68163293 None:intergenic 45.0%
TCAATTGGGCTATCTTGCTC+AGG + chr8_3:68163275-68163294 None:intergenic 45.0%
TTCTACCCTAACTGGACCAA+AGG + chr8_3:68163353-68163372 None:intergenic 45.0%
!!! TTAGTTAATTTATTTTATAA+TGG - chr8_3:68162148-68162167 Msa1304880:CDS 5.0%
!! TCATTTATAATTATATAAAT+TGG - chr8_3:68162328-68162347 Msa1304880:intron 5.0%
! GTTGTGCTGAGTTGAGGAAC+AGG + chr8_3:68159759-68159778 None:intergenic 50.0%
! GAGCTTGTTTCGAATTCCGG+GGG - chr8_3:68161144-68161163 Msa1304880:intron 50.0%
! TATTTGATGGACACGGGCCA+TGG - chr8_3:68161439-68161458 Msa1304880:intron 50.0%
! ATTTGATGGACACGGGCCAT+GGG - chr8_3:68161440-68161459 Msa1304880:intron 50.0%
TTGGCTACAAAGTGACCCCA+TGG + chr8_3:68161459-68161478 None:intergenic 50.0%
AGTCCTGGAGACTCTACATC+TGG + chr8_3:68162466-68162485 None:intergenic 50.0%
TCCAGGACTTGCCATGTCAA+GGG - chr8_3:68162477-68162496 Msa1304880:intron 50.0%
! CTTGCCATGTCAAGGGCTTT+TGG - chr8_3:68162484-68162503 Msa1304880:intron 50.0%
CAGTGGCAAGCACAACAAAC+TGG + chr8_3:68162582-68162601 None:intergenic 50.0%
TGTTGTGCTTGCCACTGATG+GGG - chr8_3:68162585-68162604 Msa1304880:intron 50.0%
GCATGGAAACGCAAGAGAAG+GGG - chr8_3:68163194-68163213 Msa1304880:intron 50.0%
GCTATCTTGCTCAGGGGAAA+TGG + chr8_3:68163267-68163286 None:intergenic 50.0%
AGACTGTTGCATGGTGTGGG+AGG - chr8_3:68161067-68161086 Msa1304880:intron 55.0%
! TTTGATGGACACGGGCCATG+GGG - chr8_3:68161441-68161460 Msa1304880:intron 55.0%
TGGTGACTCTCGTGCAGTAC+TGG - chr8_3:68162204-68162223 Msa1304880:exon 55.0%
! AACCAGGAGTGCATAGGGTC+TGG - chr8_3:68162437-68162456 Msa1304880:intron 55.0%
AACCAGACCCTATGCACTCC+TGG + chr8_3:68162442-68162461 None:intergenic 55.0%
CTCCAGGACTTGCCATGTCA+AGG - chr8_3:68162476-68162495 Msa1304880:intron 55.0%
CTTGCTCAGGGGAAATGGGA+GGG + chr8_3:68163262-68163281 None:intergenic 55.0%
TCTTGCTCAGGGGAAATGGG+AGG + chr8_3:68163263-68163282 None:intergenic 55.0%
GCCCTTGACATGGCAAGTCC+TGG + chr8_3:68162481-68162500 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 68159626 68163426 68159626 ID=Msa1304880;Name=Msa1304880
chr8_3 mRNA 68159626 68163426 68159626 ID=Msa1304880-mRNA-1;Parent=Msa1304880;Name=Msa1304880-mRNA-1;_AED=0.19;_eAED=0.19;_QI=243|0.8|0.83|1|0.8|0.66|6|119|320
chr8_3 exon 68159626 68159957 68159626 ID=Msa1304880-mRNA-1:exon:21082;Parent=Msa1304880-mRNA-1
chr8_3 exon 68160446 68160669 68160446 ID=Msa1304880-mRNA-1:exon:21081;Parent=Msa1304880-mRNA-1
chr8_3 exon 68160754 68160874 68160754 ID=Msa1304880-mRNA-1:exon:21080;Parent=Msa1304880-mRNA-1
chr8_3 exon 68161489 68161650 68161489 ID=Msa1304880-mRNA-1:exon:21079;Parent=Msa1304880-mRNA-1
chr8_3 exon 68161964 68162266 68161964 ID=Msa1304880-mRNA-1:exon:21078;Parent=Msa1304880-mRNA-1
chr8_3 exon 68163244 68163426 68163244 ID=Msa1304880-mRNA-1:exon:21077;Parent=Msa1304880-mRNA-1
chr8_3 five_prime_UTR 68163244 68163426 68163244 ID=Msa1304880-mRNA-1:five_prime_utr;Parent=Msa1304880-mRNA-1
chr8_3 five_prime_UTR 68162207 68162266 68162207 ID=Msa1304880-mRNA-1:five_prime_utr;Parent=Msa1304880-mRNA-1
chr8_3 CDS 68161964 68162206 68161964 ID=Msa1304880-mRNA-1:cds;Parent=Msa1304880-mRNA-1
chr8_3 CDS 68161489 68161650 68161489 ID=Msa1304880-mRNA-1:cds;Parent=Msa1304880-mRNA-1
chr8_3 CDS 68160754 68160874 68160754 ID=Msa1304880-mRNA-1:cds;Parent=Msa1304880-mRNA-1
chr8_3 CDS 68160446 68160669 68160446 ID=Msa1304880-mRNA-1:cds;Parent=Msa1304880-mRNA-1
chr8_3 CDS 68159745 68159957 68159745 ID=Msa1304880-mRNA-1:cds;Parent=Msa1304880-mRNA-1
chr8_3 three_prime_UTR 68159626 68159744 68159626 ID=Msa1304880-mRNA-1:three_prime_utr;Parent=Msa1304880-mRNA-1
Gene Sequence

>Msa1304880

ATGGGGCATTTATCCTCTATGTTCAATGGTTTGGCAAGATCATTTTCACTAAAGAAAGGAAGAAATTCTGTGAGATGTGAAAGGAGAGAAGCTGTTGAAGCAATGGCAAAAGAAGCTAAGAAAAATGACTTGATTTTGTGTAGCTCTGGCACTATTAATGTTGATGGTTCAAACAACTTTGCCTCAGTTTTCTCCAAAAGAGGTCAGAAAGGAACCAATCAAGACTGTTGCATGGTGTGGGAGGAATTTGGATGCCAAGAGGACATGATATTCTGCGGTATATTTGATGGACACGGGCCATGGGGTCACTTTGTAGCCAAAAGAGTAAGAGAGTCAATGCCAAGGTCTTTGCTATGCAATTGGCAAGAGACACTTGCTACACAATCTTCATCACTTGACGATCATGGGGAAACGGTTTTCATAGCAAATGTTGGTGACTCTCGTGCAGTACTGGCTACAACATCAGACGATGATGGAAGTTTGGTAGCAGTACAACTAACAGTTGATTTCAAGCCAAATTTACCTCAAGAGGAAGAGAGAATAATTCAGTGTCAAGGGCGTGTGTTCTGTTTAAATGATGAACCAGGAGTGCATAGGGTCTGGTTACCAGATGTAGAGTCTCCAGGACTTGCCATGTCAAGGGCTTTTGGAGATTATTGTATTAAAGATTATGGTCTTATTTCAGTACCTGAAGTAACTCAGAGGAATATAAGTAGTAAAGACCAGTTTGTTGTGCTTGCCACTGATGGGGTTTGGGATGTGATCTCCAATCAAGAAGCAGTAGACATTGTATCTTCAACACCAGACAAAGCAAAATCAGCTAAACGTTTGGTGGAATGTGCAGTACATGCATGGAAACGCAAGAGAAGGGGTATTGCCATTGATGATATTTCTGCTATTTGTCTCTTTTTGCACCCTCCCATTTCCCCTGAGCAAGATAGCCCAATTGAAACTGTTAAATAG
Protein sequence

>Msa1304880

MGHLSSMFNGLARSFSLKKGRNSVRCERREAVEAMAKEAKKNDLILCSSGTINVDGSNNFASVFSKRGQKGTNQDCCMVWEEFGCQEDMIFCGIFDGHGPWGHFVAKRVRESMPRSLLCNWQETLATQSSSLDDHGETVFIANVGDSRAVLATTSDDDGSLVAVQLTVDFKPNLPQEEERIIQCQGRVFCLNDEPGVHRVWLPDVESPGLAMSRAFGDYCIKDYGLISVPEVTQRNISSKDQFVVLATDGVWDVISNQEAVDIVSSTPDKAKSAKRLVECAVHAWKRKRRGIAIDDISAICLFLHPPISPEQDSPIETVK*