Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0839830 | XP_003612502.1 | 95.131 | 267 | 13 | 0 | 1 | 267 | 1 | 267 | 8.63e-160 | 454 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0839830 | G7K428 | 95.131 | 267 | 13 | 0 | 1 | 267 | 1 | 267 | 4.12e-160 | 454 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014410 | Msa0839830 | 0.832512 | 9.061589e-56 | -8.615850e-47 |
| Msa0035400 | Msa0839830 | 0.844066 | 9.512697e-59 | -8.615850e-47 |
| Msa0044710 | Msa0839830 | 0.814250 | 1.694966e-51 | -8.615850e-47 |
| Msa0064550 | Msa0839830 | 0.822680 | 2.081496e-53 | -8.615850e-47 |
| Msa0067150 | Msa0839830 | 0.819261 | 1.274548e-52 | -8.615850e-47 |
| Msa0105700 | Msa0839830 | 0.801011 | 1.100960e-48 | -8.615850e-47 |
| Msa0109490 | Msa0839830 | 0.819376 | 1.199971e-52 | -8.615850e-47 |
| Msa0111310 | Msa0839830 | 0.806128 | 9.562353e-50 | -8.615850e-47 |
| Msa0617830 | Msa0839830 | 0.829709 | 4.423149e-55 | -8.615850e-47 |
| Msa0622180 | Msa0839830 | 0.800694 | 1.278227e-48 | -8.615850e-47 |
| Msa0628100 | Msa0839830 | 0.821186 | 4.615375e-53 | -8.615850e-47 |
| Msa0629440 | Msa0839830 | -0.800404 | 1.464240e-48 | -8.615850e-47 |
| Msa0640380 | Msa0839830 | 0.812411 | 4.296638e-51 | -8.615850e-47 |
| Msa0656680 | Msa0839830 | 0.810722 | 1.000946e-50 | -8.615850e-47 |
| Msa0659890 | Msa0839830 | 0.805475 | 1.311541e-49 | -8.615850e-47 |
| Msa0661890 | Msa0839830 | 0.806941 | 6.442045e-50 | -8.615850e-47 |
| Msa0665790 | Msa0839830 | 0.800247 | 1.576064e-48 | -8.615850e-47 |
| Msa0667070 | Msa0839830 | -0.810123 | 1.347619e-50 | -8.615850e-47 |
| Msa0672120 | Msa0839830 | 0.817601 | 3.029576e-52 | -8.615850e-47 |
| Msa0676100 | Msa0839830 | 0.807178 | 5.739010e-50 | -8.615850e-47 |
| Msa0678820 | Msa0839830 | 0.812473 | 4.166283e-51 | -8.615850e-47 |
| Msa0704730 | Msa0839830 | 0.829673 | 4.513279e-55 | -8.615850e-47 |
| Msa0705420 | Msa0839830 | 0.824411 | 8.188838e-54 | -8.615850e-47 |
| Msa0706600 | Msa0839830 | 0.820784 | 5.710760e-53 | -8.615850e-47 |
| Msa0709660 | Msa0839830 | 0.808907 | 2.458503e-50 | -8.615850e-47 |
| Msa0714340 | Msa0839830 | 0.822194 | 2.699350e-53 | -8.615850e-47 |
| Msa0714380 | Msa0839830 | 0.814453 | 1.529153e-51 | -8.615850e-47 |
| Msa0715680 | Msa0839830 | 0.806401 | 8.375859e-50 | -8.615850e-47 |
| Msa0746980 | Msa0839830 | 0.820600 | 6.297272e-53 | -8.615850e-47 |
| Msa0756310 | Msa0839830 | 0.835840 | 1.327413e-56 | -8.615850e-47 |
| Msa0759970 | Msa0839830 | 0.883368 | 4.761001e-71 | -8.615850e-47 |
| Msa0795920 | Msa0839830 | 0.800917 | 1.150767e-48 | -8.615850e-47 |
| Msa0801360 | Msa0839830 | 0.996016 | 1.974091e-222 | -8.615850e-47 |
| Msa0818440 | Msa0839830 | 0.837845 | 4.087254e-57 | -8.615850e-47 |
| Msa0818550 | Msa0839830 | 0.832092 | 1.151388e-55 | -8.615850e-47 |
| Msa0828570 | Msa0839830 | 0.837161 | 6.119489e-57 | -8.615850e-47 |
| Msa0834450 | Msa0839830 | 0.802717 | 4.913110e-49 | -8.615850e-47 |
| Msa0835730 | Msa0839830 | 0.814395 | 1.575100e-51 | -8.615850e-47 |
| Msa0839830 | Msa0860920 | 0.804194 | 2.428024e-49 | -8.615850e-47 |
| Msa0839830 | Msa0877890 | 0.808480 | 3.033128e-50 | -8.615850e-47 |
| Msa0839830 | Msa0878880 | 0.830903 | 2.259234e-55 | -8.615850e-47 |
| Msa0839830 | Msa0893350 | 0.802634 | 5.112055e-49 | -8.615850e-47 |
| Msa0839830 | Msa0898860 | 0.842133 | 3.114622e-58 | -8.615850e-47 |
| Msa0839830 | Msa0928030 | 0.843773 | 1.140176e-58 | -8.615850e-47 |
| Msa0839830 | Msa0944550 | 0.837453 | 5.151941e-57 | -8.615850e-47 |
| Msa0839830 | Msa0964430 | 0.834276 | 3.290999e-56 | -8.615850e-47 |
| Msa0839830 | Msa0982270 | 0.824044 | 9.987798e-54 | -8.615850e-47 |
| Msa0839830 | Msa1012920 | 0.813095 | 3.044123e-51 | -8.615850e-47 |
| Msa0839830 | Msa1031810 | 0.833631 | 4.774411e-56 | -8.615850e-47 |
| Msa0839830 | Msa1054410 | 0.821016 | 5.052665e-53 | -8.615850e-47 |
| Msa0839830 | Msa1070850 | 0.858663 | 7.056963e-63 | -8.615850e-47 |
| Msa0839830 | Msa1075590 | 0.810258 | 1.260743e-50 | -8.615850e-47 |
| Msa0839830 | Msa1079730 | -0.832723 | 8.034699e-56 | -8.615850e-47 |
| Msa0839830 | Msa1118750 | 0.826283 | 2.953982e-54 | -8.615850e-47 |
| Msa0839830 | Msa1129210 | 0.829813 | 4.171900e-55 | -8.615850e-47 |
| Msa0839830 | Msa1141250 | -0.813204 | 2.881436e-51 | -8.615850e-47 |
| Msa0839830 | Msa1149750 | 0.813375 | 2.642637e-51 | -8.615850e-47 |
| Msa0839830 | Msa1158670 | 0.825095 | 5.649200e-54 | -8.615850e-47 |
| Msa0839830 | Msa1162980 | 0.834013 | 3.829858e-56 | -8.615850e-47 |
| Msa0839830 | Msa1168040 | -0.829682 | 4.490927e-55 | -8.615850e-47 |
| Msa0839830 | Msa1172930 | 0.802185 | 6.324387e-49 | -8.615850e-47 |
| Msa0839830 | Msa1174870 | 0.820169 | 7.904671e-53 | -8.615850e-47 |
| Msa0839830 | Msa1180050 | 0.804647 | 1.954299e-49 | -8.615850e-47 |
| Msa0839830 | Msa1187520 | 0.872555 | 2.906911e-67 | -8.615850e-47 |
| Msa0839830 | Msa1204600 | -0.810434 | 1.154771e-50 | -8.615850e-47 |
| Msa0839830 | Msa1212760 | 0.879337 | 1.353311e-69 | -8.615850e-47 |
| Msa0839830 | Msa1212980 | 0.850383 | 1.759672e-60 | -8.615850e-47 |
| Msa0839830 | Msa1232270 | 0.851779 | 7.106148e-61 | -8.615850e-47 |
| Msa0839830 | Msa1234690 | 0.807293 | 5.424463e-50 | -8.615850e-47 |
| Msa0839830 | Msa1234730 | 0.804455 | 2.142155e-49 | -8.615850e-47 |
| Msa0839830 | Msa1241820 | 0.878256 | 3.253206e-69 | -8.615850e-47 |
| Msa0839830 | Msa1241990 | 0.801689 | 7.996086e-49 | -8.615850e-47 |
| Msa0839830 | Msa1260130 | 0.859023 | 5.510564e-63 | -8.615850e-47 |
| Msa0839830 | Msa1270770 | 0.802893 | 4.518825e-49 | -8.615850e-47 |
| Msa0839830 | Msa1282920 | 0.868190 | 7.846912e-66 | -8.615850e-47 |
| Msa0839830 | Msa1292730 | 0.812516 | 4.075983e-51 | -8.615850e-47 |
| Msa0839830 | Msa1296880 | 0.803501 | 3.383210e-49 | -8.615850e-47 |
| Msa0839830 | Msa1300050 | 0.839424 | 1.598176e-57 | -8.615850e-47 |
| Msa0839830 | Msa1304880 | 0.800046 | 1.731397e-48 | -8.615850e-47 |
| Msa0839830 | Msa1307740 | 0.822246 | 2.625375e-53 | -8.615850e-47 |
| Msa0839830 | Msa1313050 | 0.843689 | 1.200994e-58 | -8.615850e-47 |
| Msa0839830 | Msa1315110 | 0.806030 | 1.002354e-49 | -8.615850e-47 |
| Msa0839830 | Msa1322190 | 0.815067 | 1.117968e-51 | -8.615850e-47 |
| Msa0839830 | Msa1354090 | 0.825722 | 4.015563e-54 | -8.615850e-47 |
| Msa0839830 | Msa1378470 | 0.830111 | 3.528732e-55 | -8.615850e-47 |
| Msa0839830 | Msa1391490 | 0.819069 | 1.409335e-52 | -8.615850e-47 |
| Msa0839830 | Msa1395440 | 0.827754 | 1.313634e-54 | -8.615850e-47 |
| Msa0839830 | Msa1398800 | 0.821846 | 3.249190e-53 | -8.615850e-47 |
| Msa0839830 | Msa1421870 | 0.824404 | 8.222244e-54 | -8.615850e-47 |
| Msa0839830 | Msa1427830 | 0.825561 | 4.383784e-54 | -8.615850e-47 |
| Msa0839830 | Msa1432790 | 0.800105 | 1.684344e-48 | -8.615850e-47 |
| Msa0839830 | Msa1434240 | 0.826275 | 2.966131e-54 | -8.615850e-47 |
| Msa0839830 | Msa1434660 | 0.801386 | 9.226006e-49 | -8.615850e-47 |
| Msa0839830 | Msa1439530 | 0.815776 | 7.773055e-52 | -8.615850e-47 |
| Msa0839830 | Msa1450020 | 0.819954 | 8.855869e-53 | -8.615850e-47 |
| Msa0839830 | Msa1454110 | 0.812952 | 3.272266e-51 | -8.615850e-47 |
| Msa0839830 | Msa1465960 | 0.814175 | 1.760991e-51 | -8.615850e-47 |
| Msa0129360 | Msa0839830 | 0.834667 | 2.625122e-56 | -8.615850e-47 |
| Msa0136390 | Msa0839830 | 0.806827 | 6.809394e-50 | -8.615850e-47 |
| Msa0142420 | Msa0839830 | 0.822881 | 1.868020e-53 | -8.615850e-47 |
| Msa0154270 | Msa0839830 | 0.821872 | 3.205369e-53 | -8.615850e-47 |
| Msa0155610 | Msa0839830 | 0.830530 | 2.788643e-55 | -8.615850e-47 |
| Msa0176040 | Msa0839830 | 0.811514 | 6.740472e-51 | -8.615850e-47 |
| Msa0178990 | Msa0839830 | 0.808268 | 3.367112e-50 | -8.615850e-47 |
| Msa0182930 | Msa0839830 | 0.804405 | 2.194679e-49 | -8.615850e-47 |
| Msa0183370 | Msa0839830 | 0.801438 | 9.003262e-49 | -8.615850e-47 |
| Msa0197180 | Msa0839830 | 0.809959 | 1.461862e-50 | -8.615850e-47 |
| Msa0198440 | Msa0839830 | 0.826568 | 2.525332e-54 | -8.615850e-47 |
| Msa0225880 | Msa0839830 | 0.817618 | 3.003842e-52 | -8.615850e-47 |
| Msa0376340 | Msa0839830 | 0.817346 | 3.458598e-52 | -8.615850e-47 |
| Msa0377280 | Msa0839830 | 0.827321 | 1.669242e-54 | -8.615850e-47 |
| Msa0379410 | Msa0839830 | 0.819702 | 1.011166e-52 | -8.615850e-47 |
| Msa0388840 | Msa0839830 | 0.845755 | 3.331497e-59 | -8.615850e-47 |
| Msa0389810 | Msa0839830 | 0.822439 | 2.368163e-53 | -8.615850e-47 |
| Msa0401340 | Msa0839830 | 0.800871 | 1.175742e-48 | -8.615850e-47 |
| Msa0404690 | Msa0839830 | 0.815269 | 1.007891e-51 | -8.615850e-47 |
| Msa0412190 | Msa0839830 | 0.843169 | 1.652958e-58 | -8.615850e-47 |
| Msa0418430 | Msa0839830 | 0.830881 | 2.287858e-55 | -8.615850e-47 |
| Msa0435010 | Msa0839830 | 0.825038 | 5.829066e-54 | -8.615850e-47 |
| Msa0442200 | Msa0839830 | 0.800432 | 1.445267e-48 | -8.615850e-47 |
| Msa0448120 | Msa0839830 | 0.805812 | 1.114017e-49 | -8.615850e-47 |
| Msa0462590 | Msa0839830 | 0.849327 | 3.471414e-60 | -8.615850e-47 |
| Msa0465800 | Msa0839830 | 0.840421 | 8.784718e-58 | -8.615850e-47 |
| Msa0468130 | Msa0839830 | 0.820647 | 6.140018e-53 | -8.615850e-47 |
| Msa0472740 | Msa0839830 | 0.849710 | 2.714522e-60 | -8.615850e-47 |
| Msa0475240 | Msa0839830 | 0.842901 | 1.948135e-58 | -8.615850e-47 |
| Msa0479100 | Msa0839830 | 0.804418 | 2.181517e-49 | -8.615850e-47 |
| Msa0240780 | Msa0839830 | 0.817248 | 3.639622e-52 | -8.615850e-47 |
| Msa0242260 | Msa0839830 | -0.803976 | 2.695152e-49 | -8.615850e-47 |
| Msa0243470 | Msa0839830 | 0.819220 | 1.302452e-52 | -8.615850e-47 |
| Msa0260470 | Msa0839830 | 0.820366 | 7.125552e-53 | -8.615850e-47 |
| Msa0278670 | Msa0839830 | 0.827279 | 1.708103e-54 | -8.615850e-47 |
| Msa0281270 | Msa0839830 | 0.808401 | 3.153697e-50 | -8.615850e-47 |
| Msa0281940 | Msa0839830 | 0.831064 | 2.063168e-55 | -8.615850e-47 |
| Msa0282170 | Msa0839830 | 0.831469 | 1.640047e-55 | -8.615850e-47 |
| Msa0304110 | Msa0839830 | 0.818140 | 2.289734e-52 | -8.615850e-47 |
| Msa0307470 | Msa0839830 | 0.832969 | 6.977807e-56 | -8.615850e-47 |
| Msa0309550 | Msa0839830 | 0.805724 | 1.162861e-49 | -8.615850e-47 |
| Msa0316750 | Msa0839830 | 0.806833 | 6.788482e-50 | -8.615850e-47 |
| Msa0318070 | Msa0839830 | 0.832049 | 1.179650e-55 | -8.615850e-47 |
| Msa0341190 | Msa0839830 | 0.821966 | 3.047884e-53 | -8.615850e-47 |
| Msa0347610 | Msa0839830 | 0.821975 | 3.033534e-53 | -8.615850e-47 |
| Msa0358290 | Msa0839830 | 0.809538 | 1.800583e-50 | -8.615850e-47 |
| Msa0489610 | Msa0839830 | 0.800903 | 1.158178e-48 | -8.615850e-47 |
| Msa0511720 | Msa0839830 | -0.800678 | 1.287367e-48 | -8.615850e-47 |
| Msa0516160 | Msa0839830 | 0.822999 | 1.753939e-53 | -8.615850e-47 |
| Msa0530950 | Msa0839830 | 0.829393 | 5.277837e-55 | -8.615850e-47 |
| Msa0543900 | Msa0839830 | 0.816394 | 5.657539e-52 | -8.615850e-47 |
| Msa0568240 | Msa0839830 | 0.803077 | 4.140502e-49 | -8.615850e-47 |
| Msa0570330 | Msa0839830 | 0.835734 | 1.412059e-56 | -8.615850e-47 |
| Msa0576600 | Msa0839830 | 0.819183 | 1.327530e-52 | -8.615850e-47 |
| Msa0577120 | Msa0839830 | 0.855841 | 4.811533e-62 | -8.615850e-47 |
| Msa0578730 | Msa0839830 | 0.802602 | 5.189662e-49 | -8.615850e-47 |
| Msa0593810 | Msa0839830 | 0.829146 | 6.060707e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0839830 | MtrunA17_Chr5g0408291 | 95.131 | 267 | 13 | 0 | 1 | 267 | 1 | 267 | 7.94e-164 | 454 |
| Msa0839830 | MtrunA17_Chr4g0034241 | 44.762 | 210 | 107 | 3 | 64 | 267 | 56 | 262 | 1.17e-55 | 179 |
| Msa0839830 | MtrunA17_Chr4g0042851 | 38.983 | 177 | 94 | 3 | 95 | 265 | 123 | 291 | 8.26e-40 | 139 |
| Msa0839830 | MtrunA17_Chr3g0089821 | 41.429 | 70 | 27 | 1 | 198 | 267 | 8 | 63 | 9.20e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0839830 | AT4G19390.1 | 65.746 | 181 | 62 | 0 | 87 | 267 | 93 | 273 | 1.84e-83 | 251 |
| Msa0839830 | AT4G19390.2 | 65.698 | 172 | 59 | 0 | 96 | 267 | 14 | 185 | 2.03e-79 | 238 |
| Msa0839830 | AT5G13720.1 | 49.198 | 187 | 79 | 4 | 92 | 267 | 80 | 261 | 2.34e-53 | 174 |
Find 57 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGATGGTGTTATCGTAAA+TGG | 0.175867 | 5_4:+15149816 | None:intergenic |
| TGTTGGGATATAAGAGTTTC+AGG | 0.199603 | 5_4:-15149740 | Msa0839830:CDS |
| TCTGATAGATCAAATCTATT+TGG | 0.202531 | 5_4:-15147981 | Msa0839830:CDS |
| TTAAACATGTATGCAAATTA+AGG | 0.228466 | 5_4:+15147614 | None:intergenic |
| CCGCTTGTTGCTTCCAATTC+GGG | 0.241751 | 5_4:-15149648 | Msa0839830:CDS |
| TGTCGGTTTCTTGCTATTCT+TGG | 0.241873 | 5_4:-15149096 | Msa0839830:CDS |
| ATTCTGTACATATTAATATT+AGG | 0.264478 | 5_4:-15147502 | Msa0839830:three_prime_UTR |
| GAGAGTCAAGGTTGTTGATT+TGG | 0.287553 | 5_4:+15149882 | None:intergenic |
| GCCGCTTGTTGCTTCCAATT+CGG | 0.314099 | 5_4:-15149649 | Msa0839830:CDS |
| TGAAATATGTTGCATGTCTT+AGG | 0.315083 | 5_4:-15147478 | Msa0839830:three_prime_UTR |
| GGAACGGTGATGCTAGTCTT+TGG | 0.339539 | 5_4:-15148071 | Msa0839830:CDS |
| AACAGAATCAAAACCCGAAT+TGG | 0.359828 | 5_4:+15149635 | None:intergenic |
| CTTCTCAGGAACGACCAAAA+TGG | 0.370797 | 5_4:-15147832 | Msa0839830:intron |
| CCTGAGAAAGTTTCATGGTT+GGG | 0.381029 | 5_4:+15149853 | None:intergenic |
| GTTTGGTGCCACTAGTTGTT+TGG | 0.381707 | 5_4:+15150007 | None:intergenic |
| TGATTCTGTTGTATCTCATC+AGG | 0.392515 | 5_4:-15149622 | Msa0839830:CDS |
| AATTCTATATTCAGCTCATA+TGG | 0.410293 | 5_4:-15149777 | Msa0839830:CDS |
| ATTTGGCTTATTCACTTTGA+AGG | 0.417143 | 5_4:-15147964 | Msa0839830:intron |
| TATTCAGCTCATATGGCAAA+AGG | 0.425103 | 5_4:-15149770 | Msa0839830:CDS |
| ATAGTGATGCTGCTTCTGAT+TGG | 0.427966 | 5_4:-15147761 | Msa0839830:CDS |
| CAATGCCTGAGAAAGTTTCA+TGG | 0.433779 | 5_4:+15149848 | None:intergenic |
| GGCAAAAGGGTCTCTGTGTT+GGG | 0.437813 | 5_4:-15149756 | Msa0839830:CDS |
| TCTATGCCTTCTAACTTCCC+TGG | 0.450735 | 5_4:+15149594 | None:intergenic |
| GAGATGCCAGGGAAGTTAGA+AGG | 0.458831 | 5_4:-15149600 | Msa0839830:CDS |
| TCATCACTCATGAGAATGTA+AGG | 0.461535 | 5_4:-15147588 | Msa0839830:three_prime_UTR |
| CATGAAACTTTCTCAGGCAT+TGG | 0.473115 | 5_4:-15149847 | Msa0839830:CDS |
| TTTGTTTGACAGAAGTAAGA+AGG | 0.477435 | 5_4:-15147738 | Msa0839830:CDS |
| TGGCAAAAGGGTCTCTGTGT+TGG | 0.477475 | 5_4:-15149757 | Msa0839830:CDS |
| TGTAGGTGATTTATAGATGT+CGG | 0.486045 | 5_4:-15149113 | Msa0839830:intron |
| TGACAGTGAATTAAAGATGA+TGG | 0.495193 | 5_4:+15149801 | None:intergenic |
| CACTATCACATGACCAACCT+TGG | 0.497716 | 5_4:+15147778 | None:intergenic |
| CCCAACCATGAAACTTTCTC+AGG | 0.502486 | 5_4:-15149853 | Msa0839830:exon |
| TTTGCTGTCCAAGCTGAATG+AGG | 0.509196 | 5_4:-15147645 | Msa0839830:CDS |
| TTAATTAGCCTCATTCAGCT+TGG | 0.510184 | 5_4:+15147637 | None:intergenic |
| CAAATCCACAGGAGTTTGAA+TGG | 0.513144 | 5_4:+15147712 | None:intergenic |
| ATCAATTCTATGCTTCATCA+AGG | 0.516481 | 5_4:-15149052 | Msa0839830:intron |
| GCCTGAGAAAGTTTCATGGT+TGG | 0.517051 | 5_4:+15149852 | None:intergenic |
| GCAATTGAATTACTTTACTA+CGG | 0.524763 | 5_4:+15148418 | None:intergenic |
| AATAGATTTGATCTATCAGA+AGG | 0.529196 | 5_4:+15147984 | None:intergenic |
| ATTCAGCTCATATGGCAAAA+GGG | 0.535020 | 5_4:-15149769 | Msa0839830:CDS |
| GTGATGCTAGTCTTTGGAAT+GGG | 0.541127 | 5_4:-15148065 | Msa0839830:CDS |
| TATCAGTGTCAAACACAAGA+TGG | 0.541440 | 5_4:+15149954 | None:intergenic |
| GAAGCAACAAGCGGCGATGA+CGG | 0.551159 | 5_4:+15149657 | None:intergenic |
| AAGTTAGAAGGCATAGAAGA+AGG | 0.553417 | 5_4:-15149588 | Msa0839830:CDS |
| CAGAAGCATAGCAAATCCAC+AGG | 0.569222 | 5_4:+15147701 | None:intergenic |
| GGTGATGCTAGTCTTTGGAA+TGG | 0.578084 | 5_4:-15148066 | Msa0839830:CDS |
| AAAAGACTCTGCAACAAATG+TGG | 0.581983 | 5_4:+15148458 | None:intergenic |
| CAATTGCTGATAGAAGCTCT+TGG | 0.584191 | 5_4:-15148402 | Msa0839830:intron |
| GTTGGGATTTCAGAGAGTCA+AGG | 0.603072 | 5_4:+15149870 | None:intergenic |
| CATAGAAGAAGGCATTGAGA+AGG | 0.605938 | 5_4:-15149577 | Msa0839830:intron |
| AATGAACTGAAAACCAAGGT+TGG | 0.611400 | 5_4:-15147791 | Msa0839830:CDS |
| AATTCTCTCCAAACAACTAG+TGG | 0.627133 | 5_4:-15150015 | Msa0839830:five_prime_UTR |
| GGCTACCATTCAAACTCCTG+TGG | 0.627628 | 5_4:-15147717 | Msa0839830:CDS |
| TATCTCATCAGGAGATGCCA+GGG | 0.638918 | 5_4:-15149611 | Msa0839830:CDS |
| CCCGAATTGGAAGCAACAAG+CGG | 0.640431 | 5_4:+15149648 | None:intergenic |
| TGGCACCAAACCAAAGACAA+AGG | 0.677264 | 5_4:-15149995 | Msa0839830:five_prime_UTR |
| AGTGAATGAACTGAAAACCA+AGG | 0.699304 | 5_4:-15147795 | Msa0839830:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATAATAACAAACAATTAA+AGG | + | chr5_4:15148276-15148295 | None:intergenic | 15.0% |
| !!! | GTCTTGTTTTTTTTTTTTTT+TGG | + | chr5_4:15148481-15148500 | None:intergenic | 15.0% |
| !!! | AACAAATTATACAAACTTTT+AGG | + | chr5_4:15149108-15149127 | None:intergenic | 15.0% |
| !! | ATTCTGTACATATTAATATT+AGG | - | chr5_4:15149960-15149979 | Msa0839830:five_prime_UTR | 15.0% |
| !! | TTATGAAAATAAGCTGAAAA+CGG | - | chr5_4:15148044-15148063 | Msa0839830:CDS | 20.0% |
| !!! | TCTATGAATAGTTTTGAATT+TGG | - | chr5_4:15148293-15148312 | Msa0839830:intron | 20.0% |
| !!! | AGATTTGCTTAACTAAATTT+GGG | + | chr5_4:15148757-15148776 | None:intergenic | 20.0% |
| !! | AATTCTTCATATAATCAACT+TGG | - | chr5_4:15149159-15149178 | Msa0839830:intron | 20.0% |
| !!! | GTTTTTATATTCAACCATTT+TGG | + | chr5_4:15149647-15149666 | None:intergenic | 20.0% |
| !! | TTAAACATGTATGCAAATTA+AGG | + | chr5_4:15149851-15149870 | None:intergenic | 20.0% |
| ! | AATTCTATATTCAGCTCATA+TGG | - | chr5_4:15147685-15147704 | Msa0839830:CDS | 25.0% |
| !!! | GCTGTTTTAAGTAGTTAATT+AGG | - | chr5_4:15148016-15148035 | Msa0839830:CDS | 25.0% |
| !!! | TTGTAATCATTGGTTTGATT+TGG | - | chr5_4:15148204-15148223 | Msa0839830:intron | 25.0% |
| !! | TCACAAAAGCATACTATTAA+AGG | + | chr5_4:15148526-15148545 | None:intergenic | 25.0% |
| !! | AATAGTATGCTTTTGTGATT+TGG | - | chr5_4:15148528-15148547 | Msa0839830:intron | 25.0% |
| ! | TATGATAGCATATTAGAATC+TGG | + | chr5_4:15148619-15148638 | None:intergenic | 25.0% |
| !! | GAGATTTGCTTAACTAAATT+TGG | + | chr5_4:15148758-15148777 | None:intergenic | 25.0% |
| ! | TTGAGAAAGTGAATAATCTT+CGG | + | chr5_4:15148875-15148894 | None:intergenic | 25.0% |
| ! | ATTATTCACTTTCTCAAACT+GGG | - | chr5_4:15148878-15148897 | Msa0839830:intron | 25.0% |
| ! | ATTATTCAATTTGACATGCA+GGG | - | chr5_4:15148979-15148998 | Msa0839830:intron | 25.0% |
| ! | GCAATTGAATTACTTTACTA+CGG | + | chr5_4:15149047-15149066 | None:intergenic | 25.0% |
| !!! | GTTAGACCTAAATTTTAACA+CGG | + | chr5_4:15149211-15149230 | None:intergenic | 25.0% |
| ! | AATAGATTTGATCTATCAGA+AGG | + | chr5_4:15149481-15149500 | None:intergenic | 25.0% |
| ! | TCTGATAGATCAAATCTATT+TGG | - | chr5_4:15149481-15149500 | Msa0839830:intron | 25.0% |
| ! | ATTTTCCTTTGTCTTTGGTT+TGG | + | chr5_4:15147475-15147494 | None:intergenic | 30.0% |
| ! | GATGAATTTTCCTTTGTCTT+TGG | + | chr5_4:15147480-15147499 | None:intergenic | 30.0% |
| TGACAGTGAATTAAAGATGA+TGG | + | chr5_4:15147664-15147683 | None:intergenic | 30.0% | |
| TTCTTCTTCGTTGTAATCAT+TGG | - | chr5_4:15148194-15148213 | Msa0839830:intron | 30.0% | |
| ! | GTTTTTCTGATGTATTGTGT+AGG | - | chr5_4:15148332-15148351 | Msa0839830:intron | 30.0% |
| TGTAGGTGATTTATAGATGT+CGG | - | chr5_4:15148349-15148368 | Msa0839830:intron | 30.0% | |
| !! | TTGCTATTCTTGGAGTTTTT+GGG | - | chr5_4:15148376-15148395 | Msa0839830:intron | 30.0% |
| ATCAATTCTATGCTTCATCA+AGG | - | chr5_4:15148410-15148429 | Msa0839830:CDS | 30.0% | |
| ACTGAATAGTAACTACAAGT+GGG | - | chr5_4:15148551-15148570 | Msa0839830:intron | 30.0% | |
| ! | ACTCTAAAAGCTATCTCATA+TGG | + | chr5_4:15148719-15148738 | None:intergenic | 30.0% |
| GATTATTCACTTTCTCAAAC+TGG | - | chr5_4:15148877-15148896 | Msa0839830:intron | 30.0% | |
| CATTATTCAATTTGACATGC+AGG | - | chr5_4:15148978-15148997 | Msa0839830:intron | 30.0% | |
| !!! | ATTTGTTGCAGAGTCTTTTT+TGG | - | chr5_4:15149008-15149027 | Msa0839830:intron | 30.0% |
| !!! | TTTGTTGCAGAGTCTTTTTT+GGG | - | chr5_4:15149009-15149028 | Msa0839830:intron | 30.0% |
| CTGATACCGTGTTAAAATTT+AGG | - | chr5_4:15149202-15149221 | Msa0839830:intron | 30.0% | |
| GTTAGGCTTAAATTTGAAGA+TGG | - | chr5_4:15149263-15149282 | Msa0839830:intron | 30.0% | |
| TGTGATTCTCTTCTTTCTAA+CGG | - | chr5_4:15149334-15149353 | Msa0839830:intron | 30.0% | |
| ! | CATGTAGATCTCTATCTTTT+AGG | - | chr5_4:15149370-15149389 | Msa0839830:intron | 30.0% |
| !! | AGATCTCTATCTTTTAGGAA+CGG | - | chr5_4:15149375-15149394 | Msa0839830:intron | 30.0% |
| ! | ATTTGGCTTATTCACTTTGA+AGG | - | chr5_4:15149498-15149517 | Msa0839830:intron | 30.0% |
| !! | AAATCCTACTGACTTTGATA+AGG | + | chr5_4:15149555-15149574 | None:intergenic | 30.0% |
| TTTGTTTGACAGAAGTAAGA+AGG | - | chr5_4:15149724-15149743 | Msa0839830:CDS | 30.0% | |
| TGAAATATGTTGCATGTCTT+AGG | - | chr5_4:15149984-15150003 | Msa0839830:five_prime_UTR | 30.0% | |
| AATTCTCTCCAAACAACTAG+TGG | - | chr5_4:15147447-15147466 | Msa0839830:three_prime_UTR | 35.0% | |
| TATCAGTGTCAAACACAAGA+TGG | + | chr5_4:15147511-15147530 | None:intergenic | 35.0% | |
| !! | GATGATGGTGTTATCGTAAA+TGG | + | chr5_4:15147649-15147668 | None:intergenic | 35.0% |
| TATTCAGCTCATATGGCAAA+AGG | - | chr5_4:15147692-15147711 | Msa0839830:CDS | 35.0% | |
| ATTCAGCTCATATGGCAAAA+GGG | - | chr5_4:15147693-15147712 | Msa0839830:CDS | 35.0% | |
| TGTTGGGATATAAGAGTTTC+AGG | - | chr5_4:15147722-15147741 | Msa0839830:CDS | 35.0% | |
| AACAGAATCAAAACCCGAAT+TGG | + | chr5_4:15147830-15147849 | None:intergenic | 35.0% | |
| TGATTCTGTTGTATCTCATC+AGG | - | chr5_4:15147840-15147859 | Msa0839830:intron | 35.0% | |
| ! | AAGTTAGAAGGCATAGAAGA+AGG | - | chr5_4:15147874-15147893 | Msa0839830:intron | 35.0% |
| !!! | CTTGCTATTCTTGGAGTTTT+TGG | - | chr5_4:15148375-15148394 | Msa0839830:intron | 35.0% |
| GACTGAATAGTAACTACAAG+TGG | - | chr5_4:15148550-15148569 | Msa0839830:intron | 35.0% | |
| TAAAAGCTAGCTCATCATTG+AGG | + | chr5_4:15148582-15148601 | None:intergenic | 35.0% | |
| AAAAGCTATCTCATATGGTG+AGG | + | chr5_4:15148714-15148733 | None:intergenic | 35.0% | |
| !!! | CTTTTAGAGTTGAGTTAAGC+TGG | - | chr5_4:15148729-15148748 | Msa0839830:intron | 35.0% |
| TTAACCCTTGGTACGAATTA+AGG | - | chr5_4:15148804-15148823 | Msa0839830:intron | 35.0% | |
| CTTTCCTTAATTCGTACCAA+GGG | + | chr5_4:15148811-15148830 | None:intergenic | 35.0% | |
| ACTTTCCTTAATTCGTACCA+AGG | + | chr5_4:15148812-15148831 | None:intergenic | 35.0% | |
| AAAAGACTCTGCAACAAATG+TGG | + | chr5_4:15149007-15149026 | None:intergenic | 35.0% | |
| !!! | AGAGTCTTTTTTGGGATACT+TGG | - | chr5_4:15149017-15149036 | Msa0839830:intron | 35.0% |
| CTAACTCATCGTTACAAAAC+TGG | + | chr5_4:15149248-15149267 | None:intergenic | 35.0% | |
| !! | CAGTTTTGTAACGATGAGTT+AGG | - | chr5_4:15149246-15149265 | Msa0839830:intron | 35.0% |
| GACAACACTAACACAAAAGT+AGG | + | chr5_4:15149293-15149312 | None:intergenic | 35.0% | |
| ! | AGTGTTGTCATTGTTCTAAG+AGG | - | chr5_4:15149304-15149323 | Msa0839830:intron | 35.0% |
| TGACTTTGATAAGGTTGACT+AGG | + | chr5_4:15149546-15149565 | None:intergenic | 35.0% | |
| TCAACCTTATCAAAGTCAGT+AGG | - | chr5_4:15149548-15149567 | Msa0839830:intron | 35.0% | |
| TCAAAGTCAGTAGGATTTAG+TGG | - | chr5_4:15149557-15149576 | Msa0839830:intron | 35.0% | |
| AGTGAATGAACTGAAAACCA+AGG | - | chr5_4:15149667-15149686 | Msa0839830:CDS | 35.0% | |
| AATGAACTGAAAACCAAGGT+TGG | - | chr5_4:15149671-15149690 | Msa0839830:CDS | 35.0% | |
| ! | TTAATTAGCCTCATTCAGCT+TGG | + | chr5_4:15149828-15149847 | None:intergenic | 35.0% |
| TCATCACTCATGAGAATGTA+AGG | - | chr5_4:15149874-15149893 | Msa0839830:five_prime_UTR | 35.0% | |
| ! | GAGAGTCAAGGTTGTTGATT+TGG | + | chr5_4:15147583-15147602 | None:intergenic | 40.0% |
| CCTGAGAAAGTTTCATGGTT+GGG | + | chr5_4:15147612-15147631 | None:intergenic | 40.0% | |
| CAATGCCTGAGAAAGTTTCA+TGG | + | chr5_4:15147617-15147636 | None:intergenic | 40.0% | |
| CATGAAACTTTCTCAGGCAT+TGG | - | chr5_4:15147615-15147634 | Msa0839830:three_prime_UTR | 40.0% | |
| !! | CATAGAAGAAGGCATTGAGA+AGG | - | chr5_4:15147885-15147904 | Msa0839830:intron | 40.0% |
| TCAAATAAGCCGATCCAAAC+TGG | - | chr5_4:15148142-15148161 | Msa0839830:intron | 40.0% | |
| GTCTTACAACCAGTTTGGAT+CGG | + | chr5_4:15148154-15148173 | None:intergenic | 40.0% | |
| CACATGTCTTACAACCAGTT+TGG | + | chr5_4:15148159-15148178 | None:intergenic | 40.0% | |
| AGACATGTGTTGAGAGTTTG+TGG | - | chr5_4:15148170-15148189 | Msa0839830:intron | 40.0% | |
| !! | TGTCGGTTTCTTGCTATTCT+TGG | - | chr5_4:15148366-15148385 | Msa0839830:intron | 40.0% |
| !!! | GGAGTTTTTGGGTCTTTGAT+TGG | - | chr5_4:15148387-15148406 | Msa0839830:intron | 40.0% |
| TCTCATATGGTGAGGTTTGT+AGG | + | chr5_4:15148706-15148725 | None:intergenic | 40.0% | |
| CCTGACATGTACTTACGAAT+TGG | + | chr5_4:15148783-15148802 | None:intergenic | 40.0% | |
| CCAATTCGTAAGTACATGTC+AGG | - | chr5_4:15148780-15148799 | Msa0839830:intron | 40.0% | |
| CAATTGCTGATAGAAGCTCT+TGG | - | chr5_4:15149060-15149079 | Msa0839830:CDS | 40.0% | |
| !! | GTGATGCTAGTCTTTGGAAT+GGG | - | chr5_4:15149397-15149416 | Msa0839830:intron | 40.0% |
| TTCCAATGTTTCTCCTTCTC+AGG | - | chr5_4:15149616-15149635 | Msa0839830:CDS | 40.0% | |
| TTCCTGAGAAGGAGAAACAT+TGG | + | chr5_4:15149621-15149640 | None:intergenic | 40.0% | |
| ! | ATAGTGATGCTGCTTCTGAT+TGG | - | chr5_4:15149701-15149720 | Msa0839830:CDS | 40.0% |
| CAAATCCACAGGAGTTTGAA+TGG | + | chr5_4:15149753-15149772 | None:intergenic | 40.0% | |
| !! | GTTTGGTGCCACTAGTTGTT+TGG | + | chr5_4:15147458-15147477 | None:intergenic | 45.0% |
| TGGCACCAAACCAAAGACAA+AGG | - | chr5_4:15147467-15147486 | Msa0839830:three_prime_UTR | 45.0% | |
| GTTGGGATTTCAGAGAGTCA+AGG | + | chr5_4:15147595-15147614 | None:intergenic | 45.0% | |
| CCCAACCATGAAACTTTCTC+AGG | - | chr5_4:15147609-15147628 | Msa0839830:three_prime_UTR | 45.0% | |
| GCCTGAGAAAGTTTCATGGT+TGG | + | chr5_4:15147613-15147632 | None:intergenic | 45.0% | |
| TATCTCATCAGGAGATGCCA+GGG | - | chr5_4:15147851-15147870 | Msa0839830:intron | 45.0% | |
| TCTATGCCTTCTAACTTCCC+TGG | + | chr5_4:15147871-15147890 | None:intergenic | 45.0% | |
| CTGCACATTTACAGGGATCT+AGG | + | chr5_4:15148652-15148671 | None:intergenic | 45.0% | |
| CACAGGACTGCACATTTACA+GGG | + | chr5_4:15148659-15148678 | None:intergenic | 45.0% | |
| TCACAGGACTGCACATTTAC+AGG | + | chr5_4:15148660-15148679 | None:intergenic | 45.0% | |
| GGTTTGTAGGACTAGACATC+CGG | + | chr5_4:15148693-15148712 | None:intergenic | 45.0% | |
| TACATGTCAGGCTTAACCCT+TGG | - | chr5_4:15148792-15148811 | Msa0839830:intron | 45.0% | |
| !! | GTATCAGAGCCTATCGATTC+GGG | + | chr5_4:15149189-15149208 | None:intergenic | 45.0% |
| ! | GGTATCAGAGCCTATCGATT+CGG | + | chr5_4:15149190-15149209 | None:intergenic | 45.0% |
| !! | GGTGATGCTAGTCTTTGGAA+TGG | - | chr5_4:15149396-15149415 | Msa0839830:intron | 45.0% |
| AGCATCAAAATGCAGCAGAG+AGG | + | chr5_4:15149594-15149613 | None:intergenic | 45.0% | |
| !! | CATTTTGGTCGTTCCTGAGA+AGG | + | chr5_4:15149632-15149651 | None:intergenic | 45.0% |
| CTTCTCAGGAACGACCAAAA+TGG | - | chr5_4:15149630-15149649 | Msa0839830:CDS | 45.0% | |
| CACTATCACATGACCAACCT+TGG | + | chr5_4:15149687-15149706 | None:intergenic | 45.0% | |
| ! | CAGAAGCATAGCAAATCCAC+AGG | + | chr5_4:15149764-15149783 | None:intergenic | 45.0% |
| ! | TTTGCTGTCCAAGCTGAATG+AGG | - | chr5_4:15149817-15149836 | Msa0839830:CDS | 45.0% |
| TGGCAAAAGGGTCTCTGTGT+TGG | - | chr5_4:15147705-15147724 | Msa0839830:CDS | 50.0% | |
| GGCAAAAGGGTCTCTGTGTT+GGG | - | chr5_4:15147706-15147725 | Msa0839830:CDS | 50.0% | |
| ! | GCCGCTTGTTGCTTCCAATT+CGG | - | chr5_4:15147813-15147832 | Msa0839830:CDS | 50.0% |
| CCCGAATTGGAAGCAACAAG+CGG | + | chr5_4:15147817-15147836 | None:intergenic | 50.0% | |
| ! | CCGCTTGTTGCTTCCAATTC+GGG | - | chr5_4:15147814-15147833 | Msa0839830:CDS | 50.0% |
| GTATCTCATCAGGAGATGCC+AGG | - | chr5_4:15147850-15147869 | Msa0839830:intron | 50.0% | |
| GAGATGCCAGGGAAGTTAGA+AGG | - | chr5_4:15147862-15147881 | Msa0839830:intron | 50.0% | |
| GTCCTGTGAATTTCACGCTC+CGG | - | chr5_4:15148671-15148690 | Msa0839830:intron | 50.0% | |
| ATCCGGAGCGTGAAATTCAC+AGG | + | chr5_4:15148676-15148695 | None:intergenic | 50.0% | |
| CTTGGATAGCCCGAATCGAT+AGG | - | chr5_4:15149177-15149196 | Msa0839830:intron | 50.0% | |
| !! | GGAACGGTGATGCTAGTCTT+TGG | - | chr5_4:15149391-15149410 | Msa0839830:intron | 50.0% |
| GGCTACCATTCAAACTCCTG+TGG | - | chr5_4:15149745-15149764 | Msa0839830:CDS | 50.0% | |
| GAAGCAACAAGCGGCGATGA+CGG | + | chr5_4:15147808-15147827 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 15147444 | 15150040 | 15147444 | ID=Msa0839830;Name=Msa0839830 |
| chr5_4 | mRNA | 15147444 | 15150040 | 15147444 | ID=Msa0839830-mRNA-1;Parent=Msa0839830;Name=Msa0839830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=172|1|1|1|1|1|5|193|267 |
| chr5_4 | exon | 15147444 | 15147846 | 15147444 | ID=Msa0839830-mRNA-1:exon:6570;Parent=Msa0839830-mRNA-1 |
| chr5_4 | exon | 15147965 | 15148107 | 15147965 | ID=Msa0839830-mRNA-1:exon:6569;Parent=Msa0839830-mRNA-1 |
| chr5_4 | exon | 15148403 | 15148484 | 15148403 | ID=Msa0839830-mRNA-1:exon:6568;Parent=Msa0839830-mRNA-1 |
| chr5_4 | exon | 15149053 | 15149130 | 15149053 | ID=Msa0839830-mRNA-1:exon:6567;Parent=Msa0839830-mRNA-1 |
| chr5_4 | exon | 15149578 | 15150040 | 15149578 | ID=Msa0839830-mRNA-1:exon:6566;Parent=Msa0839830-mRNA-1 |
| chr5_4 | five_prime_UTR | 15149869 | 15150040 | 15149869 | ID=Msa0839830-mRNA-1:five_prime_utr;Parent=Msa0839830-mRNA-1 |
| chr5_4 | CDS | 15149578 | 15149868 | 15149578 | ID=Msa0839830-mRNA-1:cds;Parent=Msa0839830-mRNA-1 |
| chr5_4 | CDS | 15149053 | 15149130 | 15149053 | ID=Msa0839830-mRNA-1:cds;Parent=Msa0839830-mRNA-1 |
| chr5_4 | CDS | 15148403 | 15148484 | 15148403 | ID=Msa0839830-mRNA-1:cds;Parent=Msa0839830-mRNA-1 |
| chr5_4 | CDS | 15147965 | 15148107 | 15147965 | ID=Msa0839830-mRNA-1:cds;Parent=Msa0839830-mRNA-1 |
| chr5_4 | CDS | 15147637 | 15147846 | 15147637 | ID=Msa0839830-mRNA-1:cds;Parent=Msa0839830-mRNA-1 |
| chr5_4 | three_prime_UTR | 15147444 | 15147636 | 15147444 | ID=Msa0839830-mRNA-1:three_prime_utr;Parent=Msa0839830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
