AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0628710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0628710 MtrunA17_Chr4g0004541 100.000 64 0 0 1 64 1 64 3.55e-37 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0628710 AT1G15270.1 89.062 64 7 0 1 64 1 64 1.12e-33 109
Msa0628710 AT3G16040.1 84.375 64 10 0 1 64 1 64 3.79e-29 98.2

Find 41 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTTGGTCGACATGGTTT+TGG 0.243730 4_3:-9384398 None:intergenic
TCTATTATGTTCTCACCTTA+TGG 0.251637 4_3:+9386135 Msa0628710:three_prime_UTR
GCCTCACAGAAGGGATCTTT+TGG 0.287259 4_3:+9385799 Msa0628710:CDS
TTCATTGTGTCTCTCCCTTT+GGG 0.288183 4_3:+9386017 Msa0628710:three_prime_UTR
TACTAATATCAGCAATTAAA+AGG 0.297547 4_3:-9385941 None:intergenic
CTTCATTGTGTCTCTCCCTT+TGG 0.300861 4_3:+9386016 Msa0628710:three_prime_UTR
TTGGGTGAAGAGTTAGGGTT+TGG 0.304226 4_3:-9384379 None:intergenic
TCTGTTTAGTGTTGTGGTTT+TGG 0.324817 4_3:+9386058 Msa0628710:three_prime_UTR
GGCTAACATCCAGAAGAAAA+AGG 0.357013 4_3:+9385605 Msa0628710:CDS
CACATCATTACGATAAGCTT+TGG 0.358073 4_3:-9385969 None:intergenic
GAAAAGTGGAAAGAAATAGA+AGG 0.393132 4_3:+9385837 Msa0628710:exon
TAATCCTTCTTATCAGACTT+TGG 0.404695 4_3:-9384630 None:intergenic
TGGGTGAAGAGTTAGGGTTT+GGG 0.436696 4_3:-9384378 None:intergenic
AAAATAATTTAGTGAGGGTA+AGG 0.451721 4_3:+9384311 Msa0628710:five_prime_UTR
AATACATCTGTTTAGTGTTG+TGG 0.454504 4_3:+9386052 Msa0628710:three_prime_UTR
AGATTGAAGGCTACCTTGCT+TGG 0.468015 4_3:-9384413 None:intergenic
TCAGACTTTGGCTTCTTCAA+AGG 0.486773 4_3:-9384618 None:intergenic
TATAGGTTGCTTATAGAAAA+CGG 0.491126 4_3:+9384240 None:intergenic
CAAGGTAAGCTGTTACCATA+AGG 0.493359 4_3:-9386150 None:intergenic
CACAATAAGAGATGTTATCA+TGG 0.496426 4_3:-9385915 None:intergenic
ATTGGTTGAACAGGCGCTAA+AGG 0.496724 4_3:+9385762 Msa0628710:intron
GAAGCCAAAGTCTGATAAGA+AGG 0.504619 4_3:+9384626 Msa0628710:CDS
GCTACCTTGCTTGGTCGACA+TGG 0.523747 4_3:-9384404 None:intergenic
TTTATCATCAAAAGCCCAAA+GGG 0.524962 4_3:-9386031 None:intergenic
TGGTAACAGCTTACCTTGTG+AGG 0.528310 4_3:+9386155 Msa0628710:three_prime_UTR
GCTAAAGGAGCTGAAGGCTA+AGG 0.540771 4_3:+9385777 Msa0628710:CDS
GGATCTGGACTCAAGAAAAG+TGG 0.544869 4_3:+9385823 Msa0628710:CDS
GAAGGCTAAGGCCTCACAGA+AGG 0.551780 4_3:+9385789 Msa0628710:CDS
AAAAGTGGAAAGAAATAGAA+GGG 0.572493 4_3:+9385838 Msa0628710:exon
AAAACCATGTCGACCAAGCA+AGG 0.588270 4_3:+9384400 Msa0628710:exon
GGTAACAGCTTACCTTGTGA+GGG 0.591622 4_3:+9386156 Msa0628710:three_prime_UTR
CAAGAATCTCATCCCTCACA+AGG 0.602683 4_3:-9386168 None:intergenic
CCTTGAAAATAATTTAGTGA+GGG 0.603194 4_3:+9384306 Msa0628710:five_prime_UTR
CCCTTGAAAATAATTTAGTG+AGG 0.613441 4_3:+9384305 Msa0628710:five_prime_UTR
AAGGCTAAGGCCTCACAGAA+GGG 0.624637 4_3:+9385790 Msa0628710:CDS
TGATAACATCTCTTATTGTG+TGG 0.632850 4_3:+9385918 Msa0628710:three_prime_UTR
ATTTATCATCAAAAGCCCAA+AGG 0.637816 4_3:-9386032 None:intergenic
ACAGGCGCTAAAGGAGCTGA+AGG 0.640092 4_3:+9385771 Msa0628710:intron
TCCAAAAGATCCCTTCTGTG+AGG 0.701774 4_3:-9385800 None:intergenic
TGATAAGAAGGATTACGATG+AGG 0.705327 4_3:+9384638 Msa0628710:CDS
AGGTTGCTTATAGAAAACGG+TGG 0.768300 4_3:+9384243 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTATTTTTTTTTTT+TGG + chr4_3:9384561-9384580 Msa0628710:intron 0.0%
!!! TTTTTTTTATTTTTTTTTTT+TGG + chr4_3:9384561-9384580 Msa0628710:intron 0.0%
!!! ATTTTAATTTTTTTTTTGTG+TGG + chr4_3:9384662-9384681 Msa0628710:intron 10.0%
!!! ATTTTAATTTTTTTTTTGTG+TGG + chr4_3:9384662-9384681 Msa0628710:intron 10.0%
!!! ATGAACAGATCTATATTTTA+GGG - chr4_3:9384523-9384542 None:intergenic 20.0%
!!! TTTTTGTGTGGATATTATAT+TGG + chr4_3:9384674-9384693 Msa0628710:intron 20.0%
!! ATTGTATATTTGAATTGTGT+TGG + chr4_3:9384703-9384722 Msa0628710:intron 20.0%
!!! TCAGTTTTACAATTATACAT+AGG + chr4_3:9385044-9385063 Msa0628710:intron 20.0%
!! AACAAAGAAAACATAAATGA+TGG - chr4_3:9385436-9385455 None:intergenic 20.0%
!!! ATTTTTGTTTCAACCATAAT+TGG + chr4_3:9385494-9385513 Msa0628710:intron 20.0%
!! ATGAAATCAAATACCAATTA+TGG - chr4_3:9385510-9385529 None:intergenic 20.0%
!!! TAATTGTATCTGTATTTTGT+AGG + chr4_3:9385575-9385594 Msa0628710:intron 20.0%
!! AAAAATAAGATCATTGTAAC+AGG - chr4_3:9385679-9385698 None:intergenic 20.0%
!! TACTAATATCAGCAATTAAA+AGG - chr4_3:9385944-9385963 None:intergenic 20.0%
!!! ATGAACAGATCTATATTTTA+GGG - chr4_3:9384523-9384542 None:intergenic 20.0%
!!! TTTTTGTGTGGATATTATAT+TGG + chr4_3:9384674-9384693 Msa0628710:intron 20.0%
!! ATTGTATATTTGAATTGTGT+TGG + chr4_3:9384703-9384722 Msa0628710:intron 20.0%
!!! TCAGTTTTACAATTATACAT+AGG + chr4_3:9385044-9385063 Msa0628710:intron 20.0%
!! AACAAAGAAAACATAAATGA+TGG - chr4_3:9385436-9385455 None:intergenic 20.0%
!!! ATTTTTGTTTCAACCATAAT+TGG + chr4_3:9385494-9385513 Msa0628710:intron 20.0%
!! ATGAAATCAAATACCAATTA+TGG - chr4_3:9385510-9385529 None:intergenic 20.0%
!!! TAATTGTATCTGTATTTTGT+AGG + chr4_3:9385575-9385594 Msa0628710:intron 20.0%
!! AAAAATAAGATCATTGTAAC+AGG - chr4_3:9385679-9385698 None:intergenic 20.0%
!! TACTAATATCAGCAATTAAA+AGG - chr4_3:9385944-9385963 None:intergenic 20.0%
!! CCTCACTAAATTATTTTCAA+GGG - chr4_3:9384308-9384327 None:intergenic 25.0%
! CCTTGAAAATAATTTAGTGA+GGG + chr4_3:9384306-9384325 Msa0628710:five_prime_UTR 25.0%
! AAAATAATTTAGTGAGGGTA+AGG + chr4_3:9384311-9384330 Msa0628710:five_prime_UTR 25.0%
!! CATGAACAGATCTATATTTT+AGG - chr4_3:9384524-9384543 None:intergenic 25.0%
!!! ACAATTGAAGTTTTCAAGTA+TGG + chr4_3:9384730-9384749 Msa0628710:intron 25.0%
!! ATGAATGTTTAGAGCTTATA+TGG + chr4_3:9385069-9385088 Msa0628710:intron 25.0%
! TCATTTATTTGGTAAGGTTT+GGG + chr4_3:9385364-9385383 Msa0628710:intron 25.0%
!! GAAAACATAAATGATGGAAT+AGG - chr4_3:9385430-9385449 None:intergenic 25.0%
! ACAGATACAATTATATCACA+AGG - chr4_3:9385568-9385587 None:intergenic 25.0%
! AAAAGTGGAAAGAAATAGAA+GGG + chr4_3:9385838-9385857 Msa0628710:exon 25.0%
!! CCTCACTAAATTATTTTCAA+GGG - chr4_3:9384308-9384327 None:intergenic 25.0%
! CCTTGAAAATAATTTAGTGA+GGG + chr4_3:9384306-9384325 Msa0628710:five_prime_UTR 25.0%
! AAAATAATTTAGTGAGGGTA+AGG + chr4_3:9384311-9384330 Msa0628710:five_prime_UTR 25.0%
!! CATGAACAGATCTATATTTT+AGG - chr4_3:9384524-9384543 None:intergenic 25.0%
!!! ACAATTGAAGTTTTCAAGTA+TGG + chr4_3:9384730-9384749 Msa0628710:intron 25.0%
!! ATGAATGTTTAGAGCTTATA+TGG + chr4_3:9385069-9385088 Msa0628710:intron 25.0%
! TCATTTATTTGGTAAGGTTT+GGG + chr4_3:9385364-9385383 Msa0628710:intron 25.0%
!! GAAAACATAAATGATGGAAT+AGG - chr4_3:9385430-9385449 None:intergenic 25.0%
! ACAGATACAATTATATCACA+AGG - chr4_3:9385568-9385587 None:intergenic 25.0%
! AAAAGTGGAAAGAAATAGAA+GGG + chr4_3:9385838-9385857 Msa0628710:exon 25.0%
CCCTTGAAAATAATTTAGTG+AGG + chr4_3:9384305-9384324 Msa0628710:five_prime_UTR 30.0%
! CCCTCACTAAATTATTTTCA+AGG - chr4_3:9384309-9384328 None:intergenic 30.0%
ACAGAGTTATAGAAGATTGA+AGG - chr4_3:9384429-9384448 None:intergenic 30.0%
!!! GGTTTGCTTTTTGTGATTTT+AGG + chr4_3:9384582-9384601 Msa0628710:intron 30.0%
!!! TTGCTTTTTGTGATTTTAGG+TGG + chr4_3:9384585-9384604 Msa0628710:intron 30.0%
TAATCCTTCTTATCAGACTT+TGG - chr4_3:9384633-9384652 None:intergenic 30.0%
!! ATTGAGCTTGGTTGTTTTAT+TGG + chr4_3:9384763-9384782 Msa0628710:intron 30.0%
!! TTGAGCTTGGTTGTTTTATT+GGG + chr4_3:9384764-9384783 Msa0628710:intron 30.0%
GAATTCAATCTATGACAACA+TGG - chr4_3:9384864-9384883 None:intergenic 30.0%
TTAGTGACCCCAAAATTAAT+AGG + chr4_3:9384960-9384979 Msa0628710:intron 30.0%
TATTAAAGTCATGACTCATG+TGG + chr4_3:9385123-9385142 Msa0628710:intron 30.0%
ATTAAAGTCATGACTCATGT+GGG + chr4_3:9385124-9385143 Msa0628710:intron 30.0%
TAGCAATCAAAAGGATTGAA+TGG - chr4_3:9385176-9385195 None:intergenic 30.0%
CACTGAATTTAGCAATCAAA+AGG - chr4_3:9385185-9385204 None:intergenic 30.0%
TTCATTTACCATACCTAGAA+TGG + chr4_3:9385221-9385240 Msa0628710:intron 30.0%
TCATTTACCATACCTAGAAT+GGG + chr4_3:9385222-9385241 Msa0628710:intron 30.0%
GTCATTTATTTGGTAAGGTT+TGG + chr4_3:9385363-9385382 Msa0628710:intron 30.0%
! TGATGGAATAGGTTTTACAA+TGG - chr4_3:9385419-9385438 None:intergenic 30.0%
! TTTTTGTTGCCTTTTACTTG+AGG + chr4_3:9385529-9385548 Msa0628710:intron 30.0%
TTACAAGATCCTCAAGTAAA+AGG - chr4_3:9385541-9385560 None:intergenic 30.0%
TTACTTGAGGATCTTGTAAA+TGG + chr4_3:9385542-9385561 Msa0628710:intron 30.0%
GAAAAGTGGAAAGAAATAGA+AGG + chr4_3:9385837-9385856 Msa0628710:exon 30.0%
CACAATAAGAGATGTTATCA+TGG - chr4_3:9385918-9385937 None:intergenic 30.0%
TGATAACATCTCTTATTGTG+TGG + chr4_3:9385918-9385937 Msa0628710:three_prime_UTR 30.0%
TTTATCATCAAAAGCCCAAA+GGG - chr4_3:9386034-9386053 None:intergenic 30.0%
ATTTATCATCAAAAGCCCAA+AGG - chr4_3:9386035-9386054 None:intergenic 30.0%
AATACATCTGTTTAGTGTTG+TGG + chr4_3:9386052-9386071 Msa0628710:three_prime_UTR 30.0%
TCTATTATGTTCTCACCTTA+TGG + chr4_3:9386135-9386154 Msa0628710:three_prime_UTR 30.0%
CCCTTGAAAATAATTTAGTG+AGG + chr4_3:9384305-9384324 Msa0628710:five_prime_UTR 30.0%
! CCCTCACTAAATTATTTTCA+AGG - chr4_3:9384309-9384328 None:intergenic 30.0%
ACAGAGTTATAGAAGATTGA+AGG - chr4_3:9384429-9384448 None:intergenic 30.0%
!!! GGTTTGCTTTTTGTGATTTT+AGG + chr4_3:9384582-9384601 Msa0628710:intron 30.0%
!!! TTGCTTTTTGTGATTTTAGG+TGG + chr4_3:9384585-9384604 Msa0628710:intron 30.0%
TAATCCTTCTTATCAGACTT+TGG - chr4_3:9384633-9384652 None:intergenic 30.0%
!! ATTGAGCTTGGTTGTTTTAT+TGG + chr4_3:9384763-9384782 Msa0628710:intron 30.0%
!! TTGAGCTTGGTTGTTTTATT+GGG + chr4_3:9384764-9384783 Msa0628710:intron 30.0%
GAATTCAATCTATGACAACA+TGG - chr4_3:9384864-9384883 None:intergenic 30.0%
TTAGTGACCCCAAAATTAAT+AGG + chr4_3:9384960-9384979 Msa0628710:intron 30.0%
TATTAAAGTCATGACTCATG+TGG + chr4_3:9385123-9385142 Msa0628710:intron 30.0%
ATTAAAGTCATGACTCATGT+GGG + chr4_3:9385124-9385143 Msa0628710:intron 30.0%
TAGCAATCAAAAGGATTGAA+TGG - chr4_3:9385176-9385195 None:intergenic 30.0%
CACTGAATTTAGCAATCAAA+AGG - chr4_3:9385185-9385204 None:intergenic 30.0%
TTCATTTACCATACCTAGAA+TGG + chr4_3:9385221-9385240 Msa0628710:intron 30.0%
TCATTTACCATACCTAGAAT+GGG + chr4_3:9385222-9385241 Msa0628710:intron 30.0%
GTCATTTATTTGGTAAGGTT+TGG + chr4_3:9385363-9385382 Msa0628710:intron 30.0%
! TGATGGAATAGGTTTTACAA+TGG - chr4_3:9385419-9385438 None:intergenic 30.0%
! TTTTTGTTGCCTTTTACTTG+AGG + chr4_3:9385529-9385548 Msa0628710:intron 30.0%
TTACAAGATCCTCAAGTAAA+AGG - chr4_3:9385541-9385560 None:intergenic 30.0%
TTACTTGAGGATCTTGTAAA+TGG + chr4_3:9385542-9385561 Msa0628710:intron 30.0%
GAAAAGTGGAAAGAAATAGA+AGG + chr4_3:9385837-9385856 Msa0628710:exon 30.0%
CACAATAAGAGATGTTATCA+TGG - chr4_3:9385918-9385937 None:intergenic 30.0%
TGATAACATCTCTTATTGTG+TGG + chr4_3:9385918-9385937 Msa0628710:three_prime_UTR 30.0%
TTTATCATCAAAAGCCCAAA+GGG - chr4_3:9386034-9386053 None:intergenic 30.0%
ATTTATCATCAAAAGCCCAA+AGG - chr4_3:9386035-9386054 None:intergenic 30.0%
AATACATCTGTTTAGTGTTG+TGG + chr4_3:9386052-9386071 Msa0628710:three_prime_UTR 30.0%
TCTATTATGTTCTCACCTTA+TGG + chr4_3:9386135-9386154 Msa0628710:three_prime_UTR 30.0%
TGATAAGAAGGATTACGATG+AGG + chr4_3:9384638-9384657 Msa0628710:CDS 35.0%
!!! TGAAGTTTTCAAGTATGGTG+CGG + chr4_3:9384735-9384754 Msa0628710:intron 35.0%
GAATTCGACAACTACTTTGT+AGG + chr4_3:9384919-9384938 Msa0628710:intron 35.0%
GACAACTACTTTGTAGGATT+AGG + chr4_3:9384925-9384944 Msa0628710:intron 35.0%
! AGACGGACCTATTAATTTTG+GGG - chr4_3:9384970-9384989 None:intergenic 35.0%
! CAGACGGACCTATTAATTTT+GGG - chr4_3:9384971-9384990 None:intergenic 35.0%
! TCAGACGGACCTATTAATTT+TGG - chr4_3:9384972-9384991 None:intergenic 35.0%
CGCTTATCTTTCTAATCAGA+CGG - chr4_3:9384987-9385006 None:intergenic 35.0%
CGTCTGATTAGAAAGATAAG+CGG + chr4_3:9384985-9385004 Msa0628710:intron 35.0%
TGGAGTTAATCATGTCATGT+AGG + chr4_3:9385089-9385108 Msa0628710:intron 35.0%
GGAGTTAATCATGTCATGTA+GGG + chr4_3:9385090-9385109 Msa0628710:intron 35.0%
GTCGAAAATTGTGTTAAGAG+TGG + chr4_3:9385277-9385296 Msa0628710:intron 35.0%
! GGTTTTGCAAGGTAAATATG+TGG + chr4_3:9385298-9385317 Msa0628710:intron 35.0%
AGACGGTCATTTATTTGGTA+AGG + chr4_3:9385358-9385377 Msa0628710:intron 35.0%
ACCCTTATTGGATATGAAGA+TGG + chr4_3:9385387-9385406 Msa0628710:intron 35.0%
GCCATCTTCATATCCAATAA+GGG - chr4_3:9385391-9385410 None:intergenic 35.0%
TGCCATCTTCATATCCAATA+AGG - chr4_3:9385392-9385411 None:intergenic 35.0%
TTATTGGATATGAAGATGGC+AGG + chr4_3:9385391-9385410 Msa0628710:intron 35.0%
TATTGGATATGAAGATGGCA+GGG + chr4_3:9385392-9385411 Msa0628710:intron 35.0%
!! CTGTATTTTGTAGGTTGACA+TGG + chr4_3:9385584-9385603 Msa0628710:intron 35.0%
TTTACCTGATTCTGTTGCAT+TGG + chr4_3:9385696-9385715 Msa0628710:intron 35.0%
TTTACCTGATTCTGTTGCAT+TGG + chr4_3:9385696-9385715 Msa0628710:intron 35.0%
CACATCATTACGATAAGCTT+TGG - chr4_3:9385972-9385991 None:intergenic 35.0%
! TCTGTTTAGTGTTGTGGTTT+TGG + chr4_3:9386058-9386077 Msa0628710:three_prime_UTR 35.0%
TTCTTGATGTTCTCACCTTA+TGG + chr4_3:9386185-9386204 Msa0628710:intron 35.0%
GAGAGATGAGATTCTTGATT+CGG + chr4_3:9386224-9386243 Msa0628710:intron 35.0%
AGAGATGAGATTCTTGATTC+GGG + chr4_3:9386225-9386244 Msa0628710:intron 35.0%
TGATAAGAAGGATTACGATG+AGG + chr4_3:9384638-9384657 Msa0628710:CDS 35.0%
!!! TGAAGTTTTCAAGTATGGTG+CGG + chr4_3:9384735-9384754 Msa0628710:intron 35.0%
GAATTCGACAACTACTTTGT+AGG + chr4_3:9384919-9384938 Msa0628710:intron 35.0%
GACAACTACTTTGTAGGATT+AGG + chr4_3:9384925-9384944 Msa0628710:intron 35.0%
! AGACGGACCTATTAATTTTG+GGG - chr4_3:9384970-9384989 None:intergenic 35.0%
! CAGACGGACCTATTAATTTT+GGG - chr4_3:9384971-9384990 None:intergenic 35.0%
! TCAGACGGACCTATTAATTT+TGG - chr4_3:9384972-9384991 None:intergenic 35.0%
CGCTTATCTTTCTAATCAGA+CGG - chr4_3:9384987-9385006 None:intergenic 35.0%
CGTCTGATTAGAAAGATAAG+CGG + chr4_3:9384985-9385004 Msa0628710:intron 35.0%
TGGAGTTAATCATGTCATGT+AGG + chr4_3:9385089-9385108 Msa0628710:intron 35.0%
GGAGTTAATCATGTCATGTA+GGG + chr4_3:9385090-9385109 Msa0628710:intron 35.0%
GTCGAAAATTGTGTTAAGAG+TGG + chr4_3:9385277-9385296 Msa0628710:intron 35.0%
! GGTTTTGCAAGGTAAATATG+TGG + chr4_3:9385298-9385317 Msa0628710:intron 35.0%
AGACGGTCATTTATTTGGTA+AGG + chr4_3:9385358-9385377 Msa0628710:intron 35.0%
ACCCTTATTGGATATGAAGA+TGG + chr4_3:9385387-9385406 Msa0628710:intron 35.0%
GCCATCTTCATATCCAATAA+GGG - chr4_3:9385391-9385410 None:intergenic 35.0%
TGCCATCTTCATATCCAATA+AGG - chr4_3:9385392-9385411 None:intergenic 35.0%
TTATTGGATATGAAGATGGC+AGG + chr4_3:9385391-9385410 Msa0628710:intron 35.0%
TATTGGATATGAAGATGGCA+GGG + chr4_3:9385392-9385411 Msa0628710:intron 35.0%
!! CTGTATTTTGTAGGTTGACA+TGG + chr4_3:9385584-9385603 Msa0628710:intron 35.0%
TTTACCTGATTCTGTTGCAT+TGG + chr4_3:9385696-9385715 Msa0628710:intron 35.0%
TTTACCTGATTCTGTTGCAT+TGG + chr4_3:9385696-9385715 Msa0628710:intron 35.0%
CACATCATTACGATAAGCTT+TGG - chr4_3:9385972-9385991 None:intergenic 35.0%
! TCTGTTTAGTGTTGTGGTTT+TGG + chr4_3:9386058-9386077 Msa0628710:three_prime_UTR 35.0%
TTCTTGATGTTCTCACCTTA+TGG + chr4_3:9386185-9386204 Msa0628710:intron 35.0%
GAGAGATGAGATTCTTGATT+CGG + chr4_3:9386224-9386243 Msa0628710:intron 35.0%
AGAGATGAGATTCTTGATTC+GGG + chr4_3:9386225-9386244 Msa0628710:intron 35.0%
!!! TGGTTTTGGGTGAAGAGTTA+GGG - chr4_3:9384387-9384406 None:intergenic 40.0%
!!! ATGGTTTTGGGTGAAGAGTT+AGG - chr4_3:9384388-9384407 None:intergenic 40.0%
! TCAGACTTTGGCTTCTTCAA+AGG - chr4_3:9384621-9384640 None:intergenic 40.0%
GAAGCCAAAGTCTGATAAGA+AGG + chr4_3:9384626-9384645 Msa0628710:CDS 40.0%
GTTAATCATGTCATGTAGGG+AGG + chr4_3:9385093-9385112 Msa0628710:intron 40.0%
TTTACCATACCTAGAATGGG+AGG + chr4_3:9385225-9385244 Msa0628710:intron 40.0%
TTACCATACCTAGAATGGGA+GGG + chr4_3:9385226-9385245 Msa0628710:intron 40.0%
!! GTGTTAAGAGTGGTTTTGCA+AGG + chr4_3:9385287-9385306 Msa0628710:intron 40.0%
TATGTGGCCACAAGTCATAT+TGG + chr4_3:9385314-9385333 Msa0628710:intron 40.0%
GAGGTAGACGGTCATTTATT+TGG + chr4_3:9385353-9385372 Msa0628710:intron 40.0%
GGCTAACATCCAGAAGAAAA+AGG + chr4_3:9385605-9385624 Msa0628710:CDS 40.0%
CATCCAGAAGAAAAAGGATG+AGG + chr4_3:9385611-9385630 Msa0628710:CDS 40.0%
! TCTCCTCATCCTTTTTCTTC+TGG - chr4_3:9385617-9385636 None:intergenic 40.0%
GAAGAAAAAGGATGAGGAGA+AGG + chr4_3:9385617-9385636 Msa0628710:CDS 40.0%
TCAACCAATGCAACAGAATC+AGG - chr4_3:9385703-9385722 None:intergenic 40.0%
TCAACCAATGCAACAGAATC+AGG - chr4_3:9385703-9385722 None:intergenic 40.0%
!! TTCTGTTGCATTGGTTGAAC+AGG + chr4_3:9385705-9385724 Msa0628710:intron 40.0%
TTCATTGTGTCTCTCCCTTT+GGG + chr4_3:9386017-9386036 Msa0628710:three_prime_UTR 40.0%
!! GTTTTGGCATAGACTTCTCT+TGG + chr4_3:9386074-9386093 Msa0628710:three_prime_UTR 40.0%
CAAGGTAAGCTGTTACCATA+AGG - chr4_3:9386153-9386172 None:intergenic 40.0%
CAAGGTAAGCTGTTACCATA+AGG - chr4_3:9386153-9386172 None:intergenic 40.0%
!!! ATTCGGGTCAATGCCATTTT+TGG + chr4_3:9386241-9386260 Msa0628710:intron 40.0%
GCAAGAGCTAATGCCAAAAA+TGG - chr4_3:9386257-9386276 None:intergenic 40.0%
!!! TGGTTTTGGGTGAAGAGTTA+GGG - chr4_3:9384387-9384406 None:intergenic 40.0%
!!! ATGGTTTTGGGTGAAGAGTT+AGG - chr4_3:9384388-9384407 None:intergenic 40.0%
! TCAGACTTTGGCTTCTTCAA+AGG - chr4_3:9384621-9384640 None:intergenic 40.0%
GAAGCCAAAGTCTGATAAGA+AGG + chr4_3:9384626-9384645 Msa0628710:CDS 40.0%
GTTAATCATGTCATGTAGGG+AGG + chr4_3:9385093-9385112 Msa0628710:intron 40.0%
TTTACCATACCTAGAATGGG+AGG + chr4_3:9385225-9385244 Msa0628710:intron 40.0%
TTACCATACCTAGAATGGGA+GGG + chr4_3:9385226-9385245 Msa0628710:intron 40.0%
!! GTGTTAAGAGTGGTTTTGCA+AGG + chr4_3:9385287-9385306 Msa0628710:intron 40.0%
TATGTGGCCACAAGTCATAT+TGG + chr4_3:9385314-9385333 Msa0628710:intron 40.0%
GAGGTAGACGGTCATTTATT+TGG + chr4_3:9385353-9385372 Msa0628710:intron 40.0%
GGCTAACATCCAGAAGAAAA+AGG + chr4_3:9385605-9385624 Msa0628710:CDS 40.0%
CATCCAGAAGAAAAAGGATG+AGG + chr4_3:9385611-9385630 Msa0628710:CDS 40.0%
! TCTCCTCATCCTTTTTCTTC+TGG - chr4_3:9385617-9385636 None:intergenic 40.0%
GAAGAAAAAGGATGAGGAGA+AGG + chr4_3:9385617-9385636 Msa0628710:CDS 40.0%
TCAACCAATGCAACAGAATC+AGG - chr4_3:9385703-9385722 None:intergenic 40.0%
TCAACCAATGCAACAGAATC+AGG - chr4_3:9385703-9385722 None:intergenic 40.0%
!! TTCTGTTGCATTGGTTGAAC+AGG + chr4_3:9385705-9385724 Msa0628710:intron 40.0%
TTCATTGTGTCTCTCCCTTT+GGG + chr4_3:9386017-9386036 Msa0628710:three_prime_UTR 40.0%
!! GTTTTGGCATAGACTTCTCT+TGG + chr4_3:9386074-9386093 Msa0628710:three_prime_UTR 40.0%
CAAGGTAAGCTGTTACCATA+AGG - chr4_3:9386153-9386172 None:intergenic 40.0%
CAAGGTAAGCTGTTACCATA+AGG - chr4_3:9386153-9386172 None:intergenic 40.0%
!!! ATTCGGGTCAATGCCATTTT+TGG + chr4_3:9386241-9386260 Msa0628710:intron 40.0%
GCAAGAGCTAATGCCAAAAA+TGG - chr4_3:9386257-9386276 None:intergenic 40.0%
TGGGTGAAGAGTTAGGGTTT+GGG - chr4_3:9384381-9384400 None:intergenic 45.0%
TTGGGTGAAGAGTTAGGGTT+TGG - chr4_3:9384382-9384401 None:intergenic 45.0%
! TGCTTGGTCGACATGGTTTT+GGG - chr4_3:9384400-9384419 None:intergenic 45.0%
! TTGCTTGGTCGACATGGTTT+TGG - chr4_3:9384401-9384420 None:intergenic 45.0%
AAAACCATGTCGACCAAGCA+AGG + chr4_3:9384400-9384419 Msa0628710:exon 45.0%
AGATTGAAGGCTACCTTGCT+TGG - chr4_3:9384416-9384435 None:intergenic 45.0%
TACCATACCTAGAATGGGAG+GGG + chr4_3:9385227-9385246 Msa0628710:intron 45.0%
CAAAGGGCCAATATGACTTG+TGG - chr4_3:9385324-9385343 None:intergenic 45.0%
CACAAGTCATATTGGCCCTT+TGG + chr4_3:9385322-9385341 Msa0628710:intron 45.0%
! TCATATTGGCCCTTTGGTGT+GGG + chr4_3:9385328-9385347 Msa0628710:intron 45.0%
! GTAAGGTTTGGGACCCTTAT+TGG + chr4_3:9385375-9385394 Msa0628710:intron 45.0%
ATTGGTTGAACAGGCGCTAA+AGG + chr4_3:9385762-9385781 Msa0628710:intron 45.0%
TCCAAAAGATCCCTTCTGTG+AGG - chr4_3:9385803-9385822 None:intergenic 45.0%
! TCACAGAAGGGATCTTTTGG+AGG + chr4_3:9385802-9385821 Msa0628710:CDS 45.0%
!! AAGGGATCTTTTGGAGGATC+TGG + chr4_3:9385808-9385827 Msa0628710:CDS 45.0%
GGATCTGGACTCAAGAAAAG+TGG + chr4_3:9385823-9385842 Msa0628710:CDS 45.0%
CTTCATTGTGTCTCTCCCTT+TGG + chr4_3:9386016-9386035 Msa0628710:three_prime_UTR 45.0%
TGGTAACAGCTTACCTTGTG+AGG + chr4_3:9386155-9386174 Msa0628710:three_prime_UTR 45.0%
GGTAACAGCTTACCTTGTGA+GGG + chr4_3:9386156-9386175 Msa0628710:three_prime_UTR 45.0%
CAAGAATCTCATCCCTCACA+AGG - chr4_3:9386171-9386190 None:intergenic 45.0%
CAAGAATCTCATCTCTCGCA+AGG - chr4_3:9386221-9386240 None:intergenic 45.0%
TGGGTGAAGAGTTAGGGTTT+GGG - chr4_3:9384381-9384400 None:intergenic 45.0%
TTGGGTGAAGAGTTAGGGTT+TGG - chr4_3:9384382-9384401 None:intergenic 45.0%
! TGCTTGGTCGACATGGTTTT+GGG - chr4_3:9384400-9384419 None:intergenic 45.0%
! TTGCTTGGTCGACATGGTTT+TGG - chr4_3:9384401-9384420 None:intergenic 45.0%
AAAACCATGTCGACCAAGCA+AGG + chr4_3:9384400-9384419 Msa0628710:exon 45.0%
AGATTGAAGGCTACCTTGCT+TGG - chr4_3:9384416-9384435 None:intergenic 45.0%
TACCATACCTAGAATGGGAG+GGG + chr4_3:9385227-9385246 Msa0628710:intron 45.0%
CAAAGGGCCAATATGACTTG+TGG - chr4_3:9385324-9385343 None:intergenic 45.0%
CACAAGTCATATTGGCCCTT+TGG + chr4_3:9385322-9385341 Msa0628710:intron 45.0%
! TCATATTGGCCCTTTGGTGT+GGG + chr4_3:9385328-9385347 Msa0628710:intron 45.0%
! GTAAGGTTTGGGACCCTTAT+TGG + chr4_3:9385375-9385394 Msa0628710:intron 45.0%
ATTGGTTGAACAGGCGCTAA+AGG + chr4_3:9385762-9385781 Msa0628710:intron 45.0%
TCCAAAAGATCCCTTCTGTG+AGG - chr4_3:9385803-9385822 None:intergenic 45.0%
! TCACAGAAGGGATCTTTTGG+AGG + chr4_3:9385802-9385821 Msa0628710:CDS 45.0%
!! AAGGGATCTTTTGGAGGATC+TGG + chr4_3:9385808-9385827 Msa0628710:CDS 45.0%
GGATCTGGACTCAAGAAAAG+TGG + chr4_3:9385823-9385842 Msa0628710:CDS 45.0%
CTTCATTGTGTCTCTCCCTT+TGG + chr4_3:9386016-9386035 Msa0628710:three_prime_UTR 45.0%
TGGTAACAGCTTACCTTGTG+AGG + chr4_3:9386155-9386174 Msa0628710:three_prime_UTR 45.0%
GGTAACAGCTTACCTTGTGA+GGG + chr4_3:9386156-9386175 Msa0628710:three_prime_UTR 45.0%
CAAGAATCTCATCCCTCACA+AGG - chr4_3:9386171-9386190 None:intergenic 45.0%
CAAGAATCTCATCTCTCGCA+AGG - chr4_3:9386221-9386240 None:intergenic 45.0%
!!! TAAAAATGTATTAATTTTAA+AGG - chr4_3:9384815-9384834 None:intergenic 5.0%
!!! AATTAATACATTTTTAATTT+TGG + chr4_3:9384819-9384838 Msa0628710:intron 5.0%
!!! ATTAATACATTTTTAATTTT+GGG + chr4_3:9384820-9384839 Msa0628710:intron 5.0%
!!! TAAAAATGTATTAATTTTAA+AGG - chr4_3:9384815-9384834 None:intergenic 5.0%
!!! AATTAATACATTTTTAATTT+TGG + chr4_3:9384819-9384838 Msa0628710:intron 5.0%
!!! ATTAATACATTTTTAATTTT+GGG + chr4_3:9384820-9384839 Msa0628710:intron 5.0%
!!! GGTGCGGTTTTGATTGAGCT+TGG + chr4_3:9384751-9384770 Msa0628710:intron 50.0%
AGCAGGATAACGAGGACTTG+AGG + chr4_3:9385251-9385270 Msa0628710:intron 50.0%
GCAGGATAACGAGGACTTGA+GGG + chr4_3:9385252-9385271 Msa0628710:intron 50.0%
GTCATATTGGCCCTTTGGTG+TGG + chr4_3:9385327-9385346 Msa0628710:intron 50.0%
! CATATTGGCCCTTTGGTGTG+GGG + chr4_3:9385329-9385348 Msa0628710:intron 50.0%
GCTAAAGGAGCTGAAGGCTA+AGG + chr4_3:9385777-9385796 Msa0628710:CDS 50.0%
AAGGCTAAGGCCTCACAGAA+GGG + chr4_3:9385790-9385809 Msa0628710:CDS 50.0%
! GCCTCACAGAAGGGATCTTT+TGG + chr4_3:9385799-9385818 Msa0628710:CDS 50.0%
!!! GGTGCGGTTTTGATTGAGCT+TGG + chr4_3:9384751-9384770 Msa0628710:intron 50.0%
AGCAGGATAACGAGGACTTG+AGG + chr4_3:9385251-9385270 Msa0628710:intron 50.0%
GCAGGATAACGAGGACTTGA+GGG + chr4_3:9385252-9385271 Msa0628710:intron 50.0%
GTCATATTGGCCCTTTGGTG+TGG + chr4_3:9385327-9385346 Msa0628710:intron 50.0%
! CATATTGGCCCTTTGGTGTG+GGG + chr4_3:9385329-9385348 Msa0628710:intron 50.0%
GCTAAAGGAGCTGAAGGCTA+AGG + chr4_3:9385777-9385796 Msa0628710:CDS 50.0%
AAGGCTAAGGCCTCACAGAA+GGG + chr4_3:9385790-9385809 Msa0628710:CDS 50.0%
! GCCTCACAGAAGGGATCTTT+TGG + chr4_3:9385799-9385818 Msa0628710:CDS 50.0%
GCTACCTTGCTTGGTCGACA+TGG - chr4_3:9384407-9384426 None:intergenic 55.0%
TGCCCCTCCCATTCTAGGTA+TGG - chr4_3:9385232-9385251 None:intergenic 55.0%
CTACCTCTCCCCACACCAAA+GGG - chr4_3:9385340-9385359 None:intergenic 55.0%
TCTACCTCTCCCCACACCAA+AGG - chr4_3:9385341-9385360 None:intergenic 55.0%
TTGGTGTGGGGAGAGGTAGA+CGG + chr4_3:9385341-9385360 Msa0628710:intron 55.0%
ACAGGCGCTAAAGGAGCTGA+AGG + chr4_3:9385771-9385790 Msa0628710:intron 55.0%
GAAGGCTAAGGCCTCACAGA+AGG + chr4_3:9385789-9385808 Msa0628710:CDS 55.0%
GCTACCTTGCTTGGTCGACA+TGG - chr4_3:9384407-9384426 None:intergenic 55.0%
TGCCCCTCCCATTCTAGGTA+TGG - chr4_3:9385232-9385251 None:intergenic 55.0%
CTACCTCTCCCCACACCAAA+GGG - chr4_3:9385340-9385359 None:intergenic 55.0%
TCTACCTCTCCCCACACCAA+AGG - chr4_3:9385341-9385360 None:intergenic 55.0%
TTGGTGTGGGGAGAGGTAGA+CGG + chr4_3:9385341-9385360 Msa0628710:intron 55.0%
ACAGGCGCTAAAGGAGCTGA+AGG + chr4_3:9385771-9385790 Msa0628710:intron 55.0%
GAAGGCTAAGGCCTCACAGA+AGG + chr4_3:9385789-9385808 Msa0628710:CDS 55.0%
CCTGCTGCCCCTCCCATTCT+AGG - chr4_3:9385237-9385256 None:intergenic 65.0%
CCTAGAATGGGAGGGGCAGC+AGG + chr4_3:9385234-9385253 Msa0628710:intron 65.0%
GGAGGGGCAGCAGGATAACG+AGG + chr4_3:9385243-9385262 Msa0628710:intron 65.0%
! TGGCCCTTTGGTGTGGGGAG+AGG + chr4_3:9385334-9385353 Msa0628710:intron 65.0%
CCTGCTGCCCCTCCCATTCT+AGG - chr4_3:9385237-9385256 None:intergenic 65.0%
CCTAGAATGGGAGGGGCAGC+AGG + chr4_3:9385234-9385253 Msa0628710:intron 65.0%
GGAGGGGCAGCAGGATAACG+AGG + chr4_3:9385243-9385262 Msa0628710:intron 65.0%
! TGGCCCTTTGGTGTGGGGAG+AGG + chr4_3:9385334-9385353 Msa0628710:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 9384254 9386298 9384254 ID=Msa0628710;Name=Msa0628710
chr4_3 mRNA 9384254 9386298 9384254 ID=Msa0628710-mRNA-1;Parent=Msa0628710;Name=Msa0628710-mRNA-1;_AED=0.02;_eAED=0.02;_QI=152|1|1|1|0.75|0.6|5|384|64
chr4_3 exon 9384254 9384421 9384254 ID=Msa0628710-mRNA-1:exon:2798;Parent=Msa0628710-mRNA-1
chr4_3 exon 9384604 9384659 9384604 ID=Msa0628710-mRNA-1:exon:2799;Parent=Msa0628710-mRNA-1
chr4_3 exon 9385597 9385638 9385597 ID=Msa0628710-mRNA-1:exon:2800;Parent=Msa0628710-mRNA-1
chr4_3 exon 9385775 9386190 9385775 ID=Msa0628710-mRNA-1:exon:2801;Parent=Msa0628710-mRNA-1
chr4_3 exon 9386250 9386298 9386250 ID=Msa0628710-mRNA-1:exon:2802;Parent=Msa0628710-mRNA-1
chr4_3 five_prime_UTR 9384254 9384405 9384254 ID=Msa0628710-mRNA-1:five_prime_utr;Parent=Msa0628710-mRNA-1
chr4_3 CDS 9384406 9384421 9384406 ID=Msa0628710-mRNA-1:cds;Parent=Msa0628710-mRNA-1
chr4_3 CDS 9384604 9384659 9384604 ID=Msa0628710-mRNA-1:cds;Parent=Msa0628710-mRNA-1
chr4_3 CDS 9385597 9385638 9385597 ID=Msa0628710-mRNA-1:cds;Parent=Msa0628710-mRNA-1
chr4_3 CDS 9385775 9385855 9385775 ID=Msa0628710-mRNA-1:cds;Parent=Msa0628710-mRNA-1
chr4_3 three_prime_UTR 9385856 9386190 9385856 ID=Msa0628710-mRNA-1:three_prime_utr;Parent=Msa0628710-mRNA-1
chr4_3 three_prime_UTR 9386250 9386298 9386250 ID=Msa0628710-mRNA-1:three_prime_utr;Parent=Msa0628710-mRNA-1
Gene Sequence

>Msa0628710

ATGTCGACCAAGCAAGGTGGAAAAGCTAAGCCTTTGAAGAAGCCAAAGTCTGATAAGAAGGATTACGATGAGGTTGACATGGCTAACATCCAGAAGAAAAAGGATGAGGAGAAGGCGCTAAAGGAGCTGAAGGCTAAGGCCTCACAGAAGGGATCTTTTGGAGGATCTGGACTCAAGAAAAGTGGAAAGAAATAG
Protein sequence

>Msa0628710

MSTKQGGKAKPLKKPKSDKKDYDEVDMANIQKKKDEEKALKELKAKASQKGSFGGSGLKKSGKK*