Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666270 | XP_024630208.1 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 8.29e-57 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666270 | A0A072TGB8 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 3.96e-57 | 182 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0009430 | Msa0666270 | 0.811503 | 6.775467e-51 | -8.615850e-47 |
| Msa0037250 | Msa0666270 | 0.803362 | 3.614271e-49 | -8.615850e-47 |
| Msa0046740 | Msa0666270 | 0.800875 | 1.173898e-48 | -8.615850e-47 |
| Msa0048150 | Msa0666270 | 0.933054 | 3.365839e-95 | -8.615850e-47 |
| Msa0054780 | Msa0666270 | 0.806651 | 7.418245e-50 | -8.615850e-47 |
| Msa0056470 | Msa0666270 | 0.833376 | 5.525101e-56 | -8.615850e-47 |
| Msa0072420 | Msa0666270 | 0.804675 | 1.928127e-49 | -8.615850e-47 |
| Msa0115990 | Msa0666270 | 0.838937 | 2.137161e-57 | -8.615850e-47 |
| Msa0607050 | Msa0666270 | 0.804833 | 1.786543e-49 | -8.615850e-47 |
| Msa0608710 | Msa0666270 | 0.819240 | 1.288394e-52 | -8.615850e-47 |
| Msa0628710 | Msa0666270 | 0.814063 | 1.864739e-51 | -8.615850e-47 |
| Msa0631350 | Msa0666270 | 0.800644 | 1.308336e-48 | -8.615850e-47 |
| Msa0634890 | Msa0666270 | 0.804069 | 2.578456e-49 | -8.615850e-47 |
| Msa0647500 | Msa0666270 | 0.863444 | 2.479545e-64 | -8.615850e-47 |
| Msa0649490 | Msa0666270 | 0.801925 | 7.152796e-49 | -8.615850e-47 |
| Msa0651050 | Msa0666270 | 0.844825 | 5.947749e-59 | -8.615850e-47 |
| Msa0661300 | Msa0666270 | 0.820877 | 5.437167e-53 | -8.615850e-47 |
| Msa0666270 | Msa0690150 | 0.830308 | 3.159796e-55 | -8.615850e-47 |
| Msa0666270 | Msa0692940 | 0.803772 | 2.972553e-49 | -8.615850e-47 |
| Msa0666270 | Msa0709400 | 0.806694 | 7.265388e-50 | -8.615850e-47 |
| Msa0666270 | Msa0788850 | 0.800852 | 1.186606e-48 | -8.615850e-47 |
| Msa0666270 | Msa1055680 | 0.805159 | 1.526808e-49 | -8.615850e-47 |
| Msa0666270 | Msa1165940 | 0.825632 | 4.216835e-54 | -8.615850e-47 |
| Msa0666270 | Msa1239910 | 0.828179 | 1.038308e-54 | -8.615850e-47 |
| Msa0666270 | Msa1244600 | 0.810526 | 1.103040e-50 | -8.615850e-47 |
| Msa0666270 | Msa1267430 | 0.812095 | 5.036300e-51 | -8.615850e-47 |
| Msa0666270 | Msa1294120 | 0.800147 | 1.652040e-48 | -8.615850e-47 |
| Msa0666270 | Msa1304820 | 0.821318 | 4.302649e-53 | -8.615850e-47 |
| Msa0666270 | Msa1305660 | 0.831732 | 1.413207e-55 | -8.615850e-47 |
| Msa0666270 | Msa1320390 | 0.830883 | 2.285456e-55 | -8.615850e-47 |
| Msa0666270 | Msa1343040 | 0.808682 | 2.746707e-50 | -8.615850e-47 |
| Msa0666270 | Msa1357620 | 0.825805 | 3.837604e-54 | -8.615850e-47 |
| Msa0666270 | Msa1361100 | 0.802531 | 5.367182e-49 | -8.615850e-47 |
| Msa0666270 | Msa1361520 | 0.807330 | 5.329378e-50 | -8.615850e-47 |
| Msa0666270 | Msa1385770 | 0.803411 | 3.530566e-49 | -8.615850e-47 |
| Msa0666270 | Msa1391410 | 0.819503 | 1.122161e-52 | -8.615850e-47 |
| Msa0666270 | Msa1403680 | 0.801781 | 7.656212e-49 | -8.615850e-47 |
| Msa0666270 | Msa1409240 | 0.800850 | 1.187884e-48 | -8.615850e-47 |
| Msa0666270 | Msa1412890 | 0.806739 | 7.106930e-50 | -8.615850e-47 |
| Msa0666270 | Msa1428880 | 0.821533 | 3.838388e-53 | -8.615850e-47 |
| Msa0666270 | Msa1432420 | 0.814445 | 1.535064e-51 | -8.615850e-47 |
| Msa0666270 | Msa1434420 | -0.824544 | 7.621812e-54 | -8.615850e-47 |
| Msa0666270 | Msa1440950 | 0.804129 | 2.504860e-49 | -8.615850e-47 |
| Msa0666270 | Msa1444740 | 0.806953 | 6.403602e-50 | -8.615850e-47 |
| Msa0666270 | Msa1451560 | 0.816092 | 6.609177e-52 | -8.615850e-47 |
| Msa0666270 | Msa1456050 | 0.814619 | 1.404711e-51 | -8.615850e-47 |
| Msa0666270 | Msa1457620 | 0.813039 | 3.132141e-51 | -8.615850e-47 |
| Msa0666270 | Msa1461280 | 0.821746 | 3.426650e-53 | -8.615850e-47 |
| Msa0123560 | Msa0666270 | 0.832732 | 7.994061e-56 | -8.615850e-47 |
| Msa0140110 | Msa0666270 | 0.810137 | 1.338613e-50 | -8.615850e-47 |
| Msa0140650 | Msa0666270 | 0.865666 | 5.004839e-65 | -8.615850e-47 |
| Msa0149190 | Msa0666270 | 0.806663 | 7.372960e-50 | -8.615850e-47 |
| Msa0149200 | Msa0666270 | 0.808375 | 3.194588e-50 | -8.615850e-47 |
| Msa0150300 | Msa0666270 | 0.833813 | 4.297562e-56 | -8.615850e-47 |
| Msa0189320 | Msa0666270 | 0.830035 | 3.683619e-55 | -8.615850e-47 |
| Msa0189520 | Msa0666270 | 0.809172 | 2.158241e-50 | -8.615850e-47 |
| Msa0190590 | Msa0666270 | 0.803904 | 2.790361e-49 | -8.615850e-47 |
| Msa0191050 | Msa0666270 | 0.835960 | 1.238044e-56 | -8.615850e-47 |
| Msa0194950 | Msa0666270 | -0.841278 | 5.234939e-58 | -8.615850e-47 |
| Msa0233350 | Msa0666270 | 0.826294 | 2.934989e-54 | -8.615850e-47 |
| Msa0236960 | Msa0666270 | -0.800568 | 1.356230e-48 | -8.615850e-47 |
| Msa0366890 | Msa0666270 | 0.835990 | 1.216376e-56 | -8.615850e-47 |
| Msa0378490 | Msa0666270 | 0.868154 | 8.062142e-66 | -8.615850e-47 |
| Msa0398670 | Msa0666270 | 0.847521 | 1.097015e-59 | -8.615850e-47 |
| Msa0414800 | Msa0666270 | 0.830958 | 2.189956e-55 | -8.615850e-47 |
| Msa0415430 | Msa0666270 | 0.811869 | 5.642987e-51 | -8.615850e-47 |
| Msa0458060 | Msa0666270 | 0.803068 | 4.159284e-49 | -8.615850e-47 |
| Msa0471590 | Msa0666270 | 0.802133 | 6.482723e-49 | -8.615850e-47 |
| Msa0477080 | Msa0666270 | 0.842224 | 2.946400e-58 | -8.615850e-47 |
| Msa0479820 | Msa0666270 | 0.814821 | 1.267502e-51 | -8.615850e-47 |
| Msa0481480 | Msa0666270 | 0.808843 | 2.537174e-50 | -8.615850e-47 |
| Msa0481810 | Msa0666270 | 0.822321 | 2.521936e-53 | -8.615850e-47 |
| Msa0267970 | Msa0666270 | 0.835481 | 1.636595e-56 | -8.615850e-47 |
| Msa0271510 | Msa0666270 | 0.805623 | 1.220792e-49 | -8.615850e-47 |
| Msa0272300 | Msa0666270 | 0.827939 | 1.185620e-54 | -8.615850e-47 |
| Msa0272450 | Msa0666270 | 0.803559 | 3.290773e-49 | -8.615850e-47 |
| Msa0273900 | Msa0666270 | 0.802128 | 6.498210e-49 | -8.615850e-47 |
| Msa0277560 | Msa0666270 | -0.805749 | 1.148680e-49 | -8.615850e-47 |
| Msa0304960 | Msa0666270 | 0.816559 | 5.195961e-52 | -8.615850e-47 |
| Msa0314720 | Msa0666270 | 0.810617 | 1.054611e-50 | -8.615850e-47 |
| Msa0315440 | Msa0666270 | -0.855535 | 5.910086e-62 | -8.615850e-47 |
| Msa0485850 | Msa0666270 | 0.806379 | 8.466306e-50 | -8.615850e-47 |
| Msa0521050 | Msa0666270 | 0.836367 | 9.753869e-57 | -8.615850e-47 |
| Msa0531530 | Msa0666270 | 0.830750 | 2.462586e-55 | -8.615850e-47 |
| Msa0531690 | Msa0666270 | 0.821895 | 3.165376e-53 | -8.615850e-47 |
| Msa0539410 | Msa0666270 | 0.810356 | 1.200579e-50 | -8.615850e-47 |
| Msa0548240 | Msa0666270 | 0.849700 | 2.732370e-60 | -8.615850e-47 |
| Msa0553050 | Msa0666270 | 0.828201 | 1.025439e-54 | -8.615850e-47 |
| Msa0555200 | Msa0666270 | 0.810238 | 1.273310e-50 | -8.615850e-47 |
| Msa0588260 | Msa0666270 | 0.834715 | 2.553063e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666270 | MtrunA17_Chr1g0173081 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 7.62e-61 | 182 |
| Msa0666270 | MtrunA17_Chr4g0004671 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 1.47e-59 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666270 | AT1G15230.1 | 43.038 | 158 | 51 | 4 | 1 | 122 | 1 | 155 | 1.58e-23 | 89.4 |
Find 40 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCTCGCCGGCCGGCTT+TGG | 0.289427 | 4_4:-6089660 | None:intergenic |
| GAGCCGGGAGCTTGTGTTGA+TGG | 0.317537 | 4_4:+6089720 | Msa0666270:CDS |
| CAGCAAACCTGGTTGTTTCT+CGG | 0.368726 | 4_4:-6089487 | None:intergenic |
| TGTTGATGGGTTGCAAGGTT+TGG | 0.372075 | 4_4:+6089734 | Msa0666270:CDS |
| AACGCTTCGTGGATTGATTC+CGG | 0.389122 | 4_4:-6089567 | None:intergenic |
| ACATAGCCACCAACCAAGAT+AGG | 0.434082 | 4_4:-6089819 | None:intergenic |
| GAAAGATGATGTAAAGGAAA+AGG | 0.441496 | 4_4:+6089758 | Msa0666270:CDS |
| CTGCTTTGAGAAACGCTTCG+TGG | 0.442668 | 4_4:-6089578 | None:intergenic |
| GAAAGAGAAGAAACCTATCT+TGG | 0.464765 | 4_4:+6089806 | Msa0666270:CDS |
| CGAAGATCCTGCACTTCCAC+CGG | 0.490722 | 4_4:+6089548 | Msa0666270:CDS |
| ATAATCTGCTGCAGCAAACC+TGG | 0.498161 | 4_4:-6089498 | None:intergenic |
| AGCCGGGAGCTTGTGTTGAT+GGG | 0.502950 | 4_4:+6089721 | Msa0666270:CDS |
| ATTGATTCCGGTGGAAGTGC+AGG | 0.512710 | 4_4:-6089555 | None:intergenic |
| TTTGGAGAAAGATGATGTAA+AGG | 0.517009 | 4_4:+6089752 | Msa0666270:CDS |
| AGAGCCGGAAAACGAGGAGC+CGG | 0.519247 | 4_4:+6089704 | Msa0666270:CDS |
| TCGAGAATATTCACATCCTC+CGG | 0.535480 | 4_4:+6089615 | Msa0666270:CDS |
| GCTTGTGTTGATGGGTTGCA+AGG | 0.538167 | 4_4:+6089729 | Msa0666270:CDS |
| GAGCCGGAAAACGAGGAGCC+GGG | 0.543615 | 4_4:+6089705 | Msa0666270:CDS |
| GAGAAGAAACCTATCTTGGT+TGG | 0.545774 | 4_4:+6089810 | Msa0666270:CDS |
| TATTCTCGATTTCACGGCGG+CGG | 0.552490 | 4_4:-6089601 | None:intergenic |
| CGATCCAAAGCCGGCCGGCG+AGG | 0.558176 | 4_4:+6089656 | Msa0666270:CDS |
| TGTCGTTGGTGTTATAGAGC+CGG | 0.561221 | 4_4:+6089689 | Msa0666270:CDS |
| TGTGAATATTCTCGATTTCA+CGG | 0.563231 | 4_4:-6089607 | None:intergenic |
| GACATCTGAAGCATCCTCGC+CGG | 0.571070 | 4_4:-6089670 | None:intergenic |
| GTCGATGCAATCATCTTCGC+CGG | 0.571440 | 4_4:-6089634 | None:intergenic |
| GAGTCCACCGAGAAACAACC+AGG | 0.578103 | 4_4:+6089480 | Msa0666270:CDS |
| TTGCATCGACGATCCAAAGC+CGG | 0.583740 | 4_4:+6089647 | Msa0666270:CDS |
| AACCCATCAACACAAGCTCC+CGG | 0.588157 | 4_4:-6089723 | None:intergenic |
| ATCGACGATCCAAAGCCGGC+CGG | 0.592029 | 4_4:+6089651 | Msa0666270:CDS |
| TCTGAAGCATCCTCGCCGGC+CGG | 0.595069 | 4_4:-6089666 | None:intergenic |
| AAGAAACCTATCTTGGTTGG+TGG | 0.604979 | 4_4:+6089813 | Msa0666270:CDS |
| GAGGATGCTTCAGATGTCGT+TGG | 0.615781 | 4_4:+6089675 | Msa0666270:CDS |
| TGTTATAGAGCCGGAAAACG+AGG | 0.616666 | 4_4:+6089698 | Msa0666270:CDS |
| CAAACCTGGTTGTTTCTCGG+TGG | 0.627408 | 4_4:-6089484 | None:intergenic |
| GCTTCGTGGATTGATTCCGG+TGG | 0.647677 | 4_4:-6089564 | None:intergenic |
| GATGCAATCATCTTCGCCGG+AGG | 0.648234 | 4_4:-6089631 | None:intergenic |
| AGACCTGCGTCTTCTAGACG+CGG | 0.716883 | 4_4:-6089525 | None:intergenic |
| CCTGCGTCTTCTAGACGCGG+TGG | 0.721425 | 4_4:-6089522 | None:intergenic |
| CCACCGCGTCTAGAAGACGC+AGG | 0.752546 | 4_4:+6089522 | Msa0666270:CDS |
| GAATATTCTCGATTTCACGG+CGG | 0.800990 | 4_4:-6089604 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGGAAAAAAGTGAAGAAA+AGG | + | chr4_4:6089776-6089795 | Msa0666270:CDS | 25.0% |
| TGTGAATATTCTCGATTTCA+CGG | - | chr4_4:6089610-6089629 | None:intergenic | 30.0% | |
| TTTGGAGAAAGATGATGTAA+AGG | + | chr4_4:6089752-6089771 | Msa0666270:CDS | 30.0% | |
| GAAAGATGATGTAAAGGAAA+AGG | + | chr4_4:6089758-6089777 | Msa0666270:CDS | 30.0% | |
| GAAAGAGAAGAAACCTATCT+TGG | + | chr4_4:6089806-6089825 | Msa0666270:CDS | 35.0% | |
| GAATATTCTCGATTTCACGG+CGG | - | chr4_4:6089607-6089626 | None:intergenic | 40.0% | |
| TCGAGAATATTCACATCCTC+CGG | + | chr4_4:6089615-6089634 | Msa0666270:CDS | 40.0% | |
| GAGAAGAAACCTATCTTGGT+TGG | + | chr4_4:6089810-6089829 | Msa0666270:CDS | 40.0% | |
| AAGAAACCTATCTTGGTTGG+TGG | + | chr4_4:6089813-6089832 | Msa0666270:CDS | 40.0% | |
| !! | CAGCAAACCTGGTTGTTTCT+CGG | - | chr4_4:6089490-6089509 | None:intergenic | 45.0% |
| ATAATCTGCTGCAGCAAACC+TGG | - | chr4_4:6089501-6089520 | None:intergenic | 45.0% | |
| ! | AACGCTTCGTGGATTGATTC+CGG | - | chr4_4:6089570-6089589 | None:intergenic | 45.0% |
| !! | TGTCGTTGGTGTTATAGAGC+CGG | + | chr4_4:6089689-6089708 | Msa0666270:CDS | 45.0% |
| TGTTATAGAGCCGGAAAACG+AGG | + | chr4_4:6089698-6089717 | Msa0666270:CDS | 45.0% | |
| !! | TGTTGATGGGTTGCAAGGTT+TGG | + | chr4_4:6089734-6089753 | Msa0666270:CDS | 45.0% |
| ACATAGCCACCAACCAAGAT+AGG | - | chr4_4:6089822-6089841 | None:intergenic | 45.0% | |
| !! | CAAACCTGGTTGTTTCTCGG+TGG | - | chr4_4:6089487-6089506 | None:intergenic | 50.0% |
| ! | ATTGATTCCGGTGGAAGTGC+AGG | - | chr4_4:6089558-6089577 | None:intergenic | 50.0% |
| !! | CTGCTTTGAGAAACGCTTCG+TGG | - | chr4_4:6089581-6089600 | None:intergenic | 50.0% |
| TATTCTCGATTTCACGGCGG+CGG | - | chr4_4:6089604-6089623 | None:intergenic | 50.0% | |
| GTCGATGCAATCATCTTCGC+CGG | - | chr4_4:6089637-6089656 | None:intergenic | 50.0% | |
| ! | TTGCATCGACGATCCAAAGC+CGG | + | chr4_4:6089647-6089666 | Msa0666270:CDS | 50.0% |
| GAGGATGCTTCAGATGTCGT+TGG | + | chr4_4:6089675-6089694 | Msa0666270:CDS | 50.0% | |
| AACCCATCAACACAAGCTCC+CGG | - | chr4_4:6089726-6089745 | None:intergenic | 50.0% | |
| !! | GCTTGTGTTGATGGGTTGCA+AGG | + | chr4_4:6089729-6089748 | Msa0666270:CDS | 50.0% |
| GAGTCCACCGAGAAACAACC+AGG | + | chr4_4:6089480-6089499 | Msa0666270:CDS | 55.0% | |
| AGACCTGCGTCTTCTAGACG+CGG | - | chr4_4:6089528-6089547 | None:intergenic | 55.0% | |
| CGAAGATCCTGCACTTCCAC+CGG | + | chr4_4:6089548-6089567 | Msa0666270:CDS | 55.0% | |
| ! | GCTTCGTGGATTGATTCCGG+TGG | - | chr4_4:6089567-6089586 | None:intergenic | 55.0% |
| GATGCAATCATCTTCGCCGG+AGG | - | chr4_4:6089634-6089653 | None:intergenic | 55.0% | |
| ! | GACATCTGAAGCATCCTCGC+CGG | - | chr4_4:6089673-6089692 | None:intergenic | 55.0% |
| ! | AGCCGGGAGCTTGTGTTGAT+GGG | + | chr4_4:6089721-6089740 | Msa0666270:CDS | 55.0% |
| ATCGACGATCCAAAGCCGGC+CGG | + | chr4_4:6089651-6089670 | Msa0666270:CDS | 60.0% | |
| AGAGCCGGAAAACGAGGAGC+CGG | + | chr4_4:6089704-6089723 | Msa0666270:CDS | 60.0% | |
| GAGCCGGGAGCTTGTGTTGA+TGG | + | chr4_4:6089720-6089739 | Msa0666270:CDS | 60.0% | |
| CCTGCGTCTTCTAGACGCGG+TGG | - | chr4_4:6089525-6089544 | None:intergenic | 65.0% | |
| CCACCGCGTCTAGAAGACGC+AGG | + | chr4_4:6089522-6089541 | Msa0666270:CDS | 65.0% | |
| ! | TCTGAAGCATCCTCGCCGGC+CGG | - | chr4_4:6089669-6089688 | None:intergenic | 65.0% |
| GAGCCGGAAAACGAGGAGCC+GGG | + | chr4_4:6089705-6089724 | Msa0666270:CDS | 65.0% | |
| ! | GCTCCCGGCTCCTCGTTTTC+CGG | - | chr4_4:6089711-6089730 | None:intergenic | 65.0% |
| CGATCCAAAGCCGGCCGGCG+AGG | + | chr4_4:6089656-6089675 | Msa0666270:CDS | 75.0% | |
| ! | GCATCCTCGCCGGCCGGCTT+TGG | - | chr4_4:6089663-6089682 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 6089477 | 6089845 | 6089477 | ID=Msa0666270;Name=Msa0666270 |
| chr4_4 | mRNA | 6089477 | 6089845 | 6089477 | ID=Msa0666270-mRNA-1;Parent=Msa0666270;Name=Msa0666270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|122 |
| chr4_4 | exon | 6089477 | 6089845 | 6089477 | ID=Msa0666270-mRNA-1:exon:2017;Parent=Msa0666270-mRNA-1 |
| chr4_4 | CDS | 6089477 | 6089845 | 6089477 | ID=Msa0666270-mRNA-1:cds;Parent=Msa0666270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
