Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0048150 | A0A072TGB8 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 3.96e-57 | 182 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0048150 | Msa0056470 | 0.816774 | 4.649329e-52 | -8.615850e-47 |
| Msa0048150 | Msa0068020 | 0.821186 | 4.615627e-53 | -8.615850e-47 |
| Msa0048150 | Msa0140650 | 0.827654 | 1.388683e-54 | -8.615850e-47 |
| Msa0048150 | Msa0190590 | 0.823464 | 1.365523e-53 | -8.615850e-47 |
| Msa0048150 | Msa0232790 | 0.802381 | 5.763500e-49 | -8.615850e-47 |
| Msa0048150 | Msa0272300 | 0.815585 | 8.572443e-52 | -8.615850e-47 |
| Msa0048150 | Msa0277560 | -0.800692 | 1.279103e-48 | -8.615850e-47 |
| Msa0048150 | Msa0315440 | -0.814631 | 1.396618e-51 | -8.615850e-47 |
| Msa0048150 | Msa0378490 | 0.854289 | 1.358890e-61 | -8.615850e-47 |
| Msa0048150 | Msa0414800 | 0.805415 | 1.349677e-49 | -8.615850e-47 |
| Msa0048150 | Msa0481810 | 0.803511 | 3.366835e-49 | -8.615850e-47 |
| Msa0048150 | Msa0521050 | 0.815565 | 8.663127e-52 | -8.615850e-47 |
| Msa0048150 | Msa0531690 | 0.809594 | 1.751677e-50 | -8.615850e-47 |
| Msa0048150 | Msa0539410 | 0.804222 | 2.395607e-49 | -8.615850e-47 |
| Msa0048150 | Msa0544540 | 0.815547 | 8.740120e-52 | -8.615850e-47 |
| Msa0048150 | Msa0548240 | 0.803055 | 4.184302e-49 | -8.615850e-47 |
| Msa0048150 | Msa0588260 | 0.826728 | 2.313767e-54 | -8.615850e-47 |
| Msa0048150 | Msa0628710 | 0.802861 | 4.588889e-49 | -8.615850e-47 |
| Msa0048150 | Msa0634890 | 0.803316 | 3.695494e-49 | -8.615850e-47 |
| Msa0048150 | Msa0647500 | 0.827768 | 1.303220e-54 | -8.615850e-47 |
| Msa0048150 | Msa0651050 | 0.818160 | 2.266303e-52 | -8.615850e-47 |
| Msa0048150 | Msa0661300 | 0.813144 | 2.969732e-51 | -8.615850e-47 |
| Msa0048150 | Msa0666270 | 0.933054 | 3.365839e-95 | -8.615850e-47 |
| Msa0048150 | Msa0690770 | 0.815894 | 7.316909e-52 | -8.615850e-47 |
| Msa0048150 | Msa0693210 | 0.808935 | 2.424831e-50 | -8.615850e-47 |
| Msa0048150 | Msa0981840 | 0.810824 | 9.509914e-51 | -8.615850e-47 |
| Msa0048150 | Msa1320390 | 0.812100 | 5.023577e-51 | -8.615850e-47 |
| Msa0048150 | Msa1413260 | 0.803779 | 2.961832e-49 | -8.615850e-47 |
| Msa0048150 | Msa1425670 | 0.802318 | 5.938208e-49 | -8.615850e-47 |
| Msa0048150 | Msa1436520 | 0.802593 | 5.212856e-49 | -8.615850e-47 |
| Msa0048150 | Msa1456050 | 0.826141 | 3.192776e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0048150 | MtrunA17_Chr1g0173081 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 7.62e-61 | 182 |
| Msa0048150 | MtrunA17_Chr4g0004671 | 84.553 | 123 | 14 | 2 | 1 | 121 | 1 | 120 | 1.47e-59 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0048150 | AT1G15230.1 | 43.038 | 158 | 51 | 4 | 1 | 122 | 1 | 155 | 1.58e-23 | 89.4 |
Find 40 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCTCGCCGGCCGGCTT+TGG | 0.289427 | 1_2:+367976 | None:intergenic |
| GAGCCGGGAGCTTGTGTTGA+TGG | 0.317537 | 1_2:-367916 | Msa0048150:CDS |
| CAGCAAACCTGGTTGTTTCT+CGG | 0.368726 | 1_2:+368149 | None:intergenic |
| TGTTGATGGGTTGCAAGGTT+TGG | 0.372075 | 1_2:-367902 | Msa0048150:CDS |
| AACGCTTCGTGGATTGATTC+CGG | 0.389122 | 1_2:+368069 | None:intergenic |
| ACATAGCCACCAACCAAGAT+AGG | 0.434082 | 1_2:+367817 | None:intergenic |
| GAAAGATGATGTAAAGGAAA+AGG | 0.441496 | 1_2:-367878 | Msa0048150:CDS |
| CTGCTTTGAGAAACGCTTCG+TGG | 0.442668 | 1_2:+368058 | None:intergenic |
| GAAAGAGAAGAAACCTATCT+TGG | 0.464765 | 1_2:-367830 | Msa0048150:CDS |
| CGAAGATCCTGCACTTCCAC+CGG | 0.490722 | 1_2:-368088 | Msa0048150:CDS |
| ATAATCTGCTGCAGCAAACC+TGG | 0.498161 | 1_2:+368138 | None:intergenic |
| AGCCGGGAGCTTGTGTTGAT+GGG | 0.502950 | 1_2:-367915 | Msa0048150:CDS |
| ATTGATTCCGGTGGAAGTGC+AGG | 0.512710 | 1_2:+368081 | None:intergenic |
| TTTGGAGAAAGATGATGTAA+AGG | 0.517009 | 1_2:-367884 | Msa0048150:CDS |
| AGAGCCGGAAAACGAGGAGC+CGG | 0.519247 | 1_2:-367932 | Msa0048150:CDS |
| TCGAGAATATTCACATCCTC+CGG | 0.535480 | 1_2:-368021 | Msa0048150:CDS |
| GCTTGTGTTGATGGGTTGCA+AGG | 0.538167 | 1_2:-367907 | Msa0048150:CDS |
| GAGCCGGAAAACGAGGAGCC+GGG | 0.543615 | 1_2:-367931 | Msa0048150:CDS |
| GAGAAGAAACCTATCTTGGT+TGG | 0.545774 | 1_2:-367826 | Msa0048150:CDS |
| TATTCTCGATTTCACGGCGG+CGG | 0.552490 | 1_2:+368035 | None:intergenic |
| CGATCCAAAGCCGGCCGGCG+AGG | 0.558176 | 1_2:-367980 | Msa0048150:CDS |
| TGTCGTTGGTGTTATAGAGC+CGG | 0.561221 | 1_2:-367947 | Msa0048150:CDS |
| TGTGAATATTCTCGATTTCA+CGG | 0.563231 | 1_2:+368029 | None:intergenic |
| GACATCTGAAGCATCCTCGC+CGG | 0.571070 | 1_2:+367966 | None:intergenic |
| GTCGATGCAATCATCTTCGC+CGG | 0.571440 | 1_2:+368002 | None:intergenic |
| GAGTCCACCGAGAAACAACC+AGG | 0.578103 | 1_2:-368156 | Msa0048150:CDS |
| TTGCATCGACGATCCAAAGC+CGG | 0.583740 | 1_2:-367989 | Msa0048150:CDS |
| AACCCATCAACACAAGCTCC+CGG | 0.588157 | 1_2:+367913 | None:intergenic |
| ATCGACGATCCAAAGCCGGC+CGG | 0.592029 | 1_2:-367985 | Msa0048150:CDS |
| TCTGAAGCATCCTCGCCGGC+CGG | 0.595069 | 1_2:+367970 | None:intergenic |
| AAGAAACCTATCTTGGTTGG+TGG | 0.604979 | 1_2:-367823 | Msa0048150:CDS |
| GAGGATGCTTCAGATGTCGT+TGG | 0.615781 | 1_2:-367961 | Msa0048150:CDS |
| TGTTATAGAGCCGGAAAACG+AGG | 0.616666 | 1_2:-367938 | Msa0048150:CDS |
| CAAACCTGGTTGTTTCTCGG+TGG | 0.627408 | 1_2:+368152 | None:intergenic |
| GCTTCGTGGATTGATTCCGG+TGG | 0.647677 | 1_2:+368072 | None:intergenic |
| GATGCAATCATCTTCGCCGG+AGG | 0.648234 | 1_2:+368005 | None:intergenic |
| AGACCTGCGTCTTCTAGACG+CGG | 0.716883 | 1_2:+368111 | None:intergenic |
| CCTGCGTCTTCTAGACGCGG+TGG | 0.721425 | 1_2:+368114 | None:intergenic |
| CCACCGCGTCTAGAAGACGC+AGG | 0.752546 | 1_2:-368114 | Msa0048150:CDS |
| GAATATTCTCGATTTCACGG+CGG | 0.800990 | 1_2:+368032 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGGAAAAAAGTGAAGAAA+AGG | - | chr1_2:368112-368131 | Msa0048150:CDS | 25.0% |
| TGTGAATATTCTCGATTTCA+CGG | + | chr1_2:367946-367965 | None:intergenic | 30.0% | |
| TTTGGAGAAAGATGATGTAA+AGG | - | chr1_2:368088-368107 | Msa0048150:CDS | 30.0% | |
| GAAAGATGATGTAAAGGAAA+AGG | - | chr1_2:368094-368113 | Msa0048150:CDS | 30.0% | |
| GAAAGAGAAGAAACCTATCT+TGG | - | chr1_2:368142-368161 | Msa0048150:CDS | 35.0% | |
| GAATATTCTCGATTTCACGG+CGG | + | chr1_2:367943-367962 | None:intergenic | 40.0% | |
| TCGAGAATATTCACATCCTC+CGG | - | chr1_2:367951-367970 | Msa0048150:CDS | 40.0% | |
| GAGAAGAAACCTATCTTGGT+TGG | - | chr1_2:368146-368165 | Msa0048150:CDS | 40.0% | |
| AAGAAACCTATCTTGGTTGG+TGG | - | chr1_2:368149-368168 | Msa0048150:CDS | 40.0% | |
| !! | CAGCAAACCTGGTTGTTTCT+CGG | + | chr1_2:367826-367845 | None:intergenic | 45.0% |
| ATAATCTGCTGCAGCAAACC+TGG | + | chr1_2:367837-367856 | None:intergenic | 45.0% | |
| ! | AACGCTTCGTGGATTGATTC+CGG | + | chr1_2:367906-367925 | None:intergenic | 45.0% |
| !! | TGTCGTTGGTGTTATAGAGC+CGG | - | chr1_2:368025-368044 | Msa0048150:CDS | 45.0% |
| TGTTATAGAGCCGGAAAACG+AGG | - | chr1_2:368034-368053 | Msa0048150:CDS | 45.0% | |
| !! | TGTTGATGGGTTGCAAGGTT+TGG | - | chr1_2:368070-368089 | Msa0048150:CDS | 45.0% |
| ACATAGCCACCAACCAAGAT+AGG | + | chr1_2:368158-368177 | None:intergenic | 45.0% | |
| !! | CAAACCTGGTTGTTTCTCGG+TGG | + | chr1_2:367823-367842 | None:intergenic | 50.0% |
| ! | ATTGATTCCGGTGGAAGTGC+AGG | + | chr1_2:367894-367913 | None:intergenic | 50.0% |
| !! | CTGCTTTGAGAAACGCTTCG+TGG | + | chr1_2:367917-367936 | None:intergenic | 50.0% |
| TATTCTCGATTTCACGGCGG+CGG | + | chr1_2:367940-367959 | None:intergenic | 50.0% | |
| GTCGATGCAATCATCTTCGC+CGG | + | chr1_2:367973-367992 | None:intergenic | 50.0% | |
| ! | TTGCATCGACGATCCAAAGC+CGG | - | chr1_2:367983-368002 | Msa0048150:CDS | 50.0% |
| GAGGATGCTTCAGATGTCGT+TGG | - | chr1_2:368011-368030 | Msa0048150:CDS | 50.0% | |
| AACCCATCAACACAAGCTCC+CGG | + | chr1_2:368062-368081 | None:intergenic | 50.0% | |
| !! | GCTTGTGTTGATGGGTTGCA+AGG | - | chr1_2:368065-368084 | Msa0048150:CDS | 50.0% |
| GAGTCCACCGAGAAACAACC+AGG | - | chr1_2:367816-367835 | Msa0048150:CDS | 55.0% | |
| AGACCTGCGTCTTCTAGACG+CGG | + | chr1_2:367864-367883 | None:intergenic | 55.0% | |
| CGAAGATCCTGCACTTCCAC+CGG | - | chr1_2:367884-367903 | Msa0048150:CDS | 55.0% | |
| ! | GCTTCGTGGATTGATTCCGG+TGG | + | chr1_2:367903-367922 | None:intergenic | 55.0% |
| GATGCAATCATCTTCGCCGG+AGG | + | chr1_2:367970-367989 | None:intergenic | 55.0% | |
| ! | GACATCTGAAGCATCCTCGC+CGG | + | chr1_2:368009-368028 | None:intergenic | 55.0% |
| ! | AGCCGGGAGCTTGTGTTGAT+GGG | - | chr1_2:368057-368076 | Msa0048150:CDS | 55.0% |
| ATCGACGATCCAAAGCCGGC+CGG | - | chr1_2:367987-368006 | Msa0048150:CDS | 60.0% | |
| AGAGCCGGAAAACGAGGAGC+CGG | - | chr1_2:368040-368059 | Msa0048150:CDS | 60.0% | |
| GAGCCGGGAGCTTGTGTTGA+TGG | - | chr1_2:368056-368075 | Msa0048150:CDS | 60.0% | |
| CCTGCGTCTTCTAGACGCGG+TGG | + | chr1_2:367861-367880 | None:intergenic | 65.0% | |
| CCACCGCGTCTAGAAGACGC+AGG | - | chr1_2:367858-367877 | Msa0048150:CDS | 65.0% | |
| ! | TCTGAAGCATCCTCGCCGGC+CGG | + | chr1_2:368005-368024 | None:intergenic | 65.0% |
| GAGCCGGAAAACGAGGAGCC+GGG | - | chr1_2:368041-368060 | Msa0048150:CDS | 65.0% | |
| ! | GCTCCCGGCTCCTCGTTTTC+CGG | + | chr1_2:368047-368066 | None:intergenic | 65.0% |
| CGATCCAAAGCCGGCCGGCG+AGG | - | chr1_2:367992-368011 | Msa0048150:CDS | 75.0% | |
| ! | GCATCCTCGCCGGCCGGCTT+TGG | + | chr1_2:367999-368018 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 367813 | 368181 | 367813 | ID=Msa0048150;Name=Msa0048150 |
| chr1_2 | mRNA | 367813 | 368181 | 367813 | ID=Msa0048150-mRNA-1;Parent=Msa0048150;Name=Msa0048150-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|122 |
| chr1_2 | exon | 367813 | 368181 | 367813 | ID=Msa0048150-mRNA-1:exon:18;Parent=Msa0048150-mRNA-1 |
| chr1_2 | CDS | 367813 | 368181 | 367813 | ID=Msa0048150-mRNA-1:cds;Parent=Msa0048150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
