AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0633240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTAACCAACAAGAATT+TGG 0.215065 4_3:-18633471 Msa0633240:five_prime_UTR
AGTTCACTTTCTCAACGTTT+TGG 0.264629 4_3:+18632728 None:intergenic
TGAAGGTGATTCTTGTGTTT+TGG 0.303850 4_3:-18633553 Msa0633240:five_prime_UTR
TTGCACCTACAGTTTCATTA+TGG 0.321692 4_3:-18632221 Msa0633240:three_prime_UTR
ACTGTTGGTGTCGGTGTTGC+TGG 0.340769 4_3:-18633320 Msa0633240:CDS
TTAGTGTTCTGGTGCAAATC+AGG 0.390681 4_3:-18632388 Msa0633240:intron
TGTGTTGAGATTTAGTGTTC+TGG 0.393710 4_3:-18632399 Msa0633240:exon
CGATGTCGGCGAAGTTAGTT+CGG 0.393719 4_3:-18633366 Msa0633240:CDS
TTCATCACATTGGCAATCAT+TGG 0.396788 4_3:+18631438 None:intergenic
TCCATTTGCACCTACACTCC+TGG 0.409884 4_3:-18632246 Msa0633240:three_prime_UTR
GAAATGAAACATTGTTCTAA+AGG 0.420124 4_3:-18632182 Msa0633240:intron
AGAAAGTGAACTGGGAAACT+TGG 0.423349 4_3:-18632717 Msa0633240:CDS
TATTGCTCTGGTTGTACTCT+CGG 0.424030 4_3:-18633297 Msa0633240:CDS
CTTAAGAAGTGTAATTATGA+AGG 0.426449 4_3:-18633415 Msa0633240:five_prime_UTR
ACCAGGAGTGTAGGTGCAAA+TGG 0.432206 4_3:+18632245 None:intergenic
CGGTGTTGCTGGTATTGCTC+TGG 0.450550 4_3:-18633309 Msa0633240:CDS
TGGTTAATCTCATATACTTA+CGG 0.460145 4_3:+18633483 None:intergenic
TGAGGTGGTTTCCTATGATA+TGG 0.460675 4_3:-18632422 Msa0633240:CDS
TTATGGCAAGTGACAAGATC+AGG 0.462078 4_3:-18632204 Msa0633240:three_prime_UTR
ATCTCAACACACCATATCAT+AGG 0.464654 4_3:+18632411 None:intergenic
CATTTCCCTCATCAACATAT+CGG 0.470848 4_3:-18632866 Msa0633240:CDS
AAAACGTTGAGAAAGTGAAC+TGG 0.473660 4_3:-18632726 Msa0633240:CDS
AACGCTAATGTTATCACTGT+TGG 0.479387 4_3:-18633335 Msa0633240:CDS
CTCATCAACATATCGGCATC+AGG 0.481451 4_3:-18632859 Msa0633240:intron
GCAGTTATCAACATCTTCGT+TGG 0.491361 4_3:-18632903 Msa0633240:intron
ACTTCTTAAGCATCTTTACA+AGG 0.508307 4_3:+18633428 None:intergenic
GTTGGATGCCAAATTCTTGT+TGG 0.510438 4_3:+18633463 None:intergenic
CAGGCATAAGCAAGTGATGT+AGG 0.512448 4_3:-18631464 Msa0633240:three_prime_UTR
ACAAGGATCTTAAGCCATGT+TGG 0.517216 4_3:+18633445 None:intergenic
ATTGCTCTGGTTGTACTCTC+GGG 0.525362 4_3:-18633296 Msa0633240:CDS
CAAGAATTTGGCATCCAACA+TGG 0.531190 4_3:-18633459 Msa0633240:five_prime_UTR
TTCTTGAGGCTTCATCACAT+TGG 0.566647 4_3:+18631428 None:intergenic
CTCTGGTTGTACTCTCGGGA+AGG 0.577901 4_3:-18633292 Msa0633240:intron
GATTAGAGTTACTCATGAGG+TGG 0.581584 4_3:-18632437 Msa0633240:CDS
AGGTGCAAAACCAGGAGTGT+AGG 0.604566 4_3:+18632236 None:intergenic
AATGTTATCACTGTTGGTGT+CGG 0.605324 4_3:-18633329 Msa0633240:CDS
AAACGTTGAGAAAGTGAACT+GGG 0.630136 4_3:-18632725 Msa0633240:CDS
GAAACTGTAGGTGCAAAACC+AGG 0.636631 4_3:+18632228 None:intergenic
ATTGTGACGATTATCGATGT+CGG 0.639449 4_3:-18633380 Msa0633240:exon
CTGATGCCGATATGTTGATG+AGG 0.659856 4_3:+18632860 None:intergenic
GTGAACTGGGAAACTTGGAT+AGG 0.670748 4_3:-18632712 Msa0633240:intron
GTATTCAGGAGATATTCTTG+AGG 0.672009 4_3:+18631414 None:intergenic
TGATGCCGATATGTTGATGA+GGG 0.679239 4_3:+18632861 None:intergenic
ACTTGCCATAATGAAACTGT+AGG 0.704994 4_3:+18632216 None:intergenic
TCTGATTAGAGTTACTCATG+AGG 0.713055 4_3:-18632440 Msa0633240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGATTGATTTTATATTT+GGG - chr4_3:18631857-18631876 Msa0633240:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG - chr4_3:18632456-18632475 Msa0633240:CDS 10.0%
!!! TATAGATTGATTTTATATTT+GGG - chr4_3:18631857-18631876 Msa0633240:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG - chr4_3:18632456-18632475 Msa0633240:CDS 10.0%
!!! AAGTTTTGATTTGATTTATT+TGG - chr4_3:18631585-18631604 Msa0633240:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG - chr4_3:18631856-18631875 Msa0633240:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG + chr4_3:18632442-18632461 None:intergenic 15.0%
!!! AAGTTTTGATTTGATTTATT+TGG - chr4_3:18631585-18631604 Msa0633240:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG - chr4_3:18631856-18631875 Msa0633240:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG + chr4_3:18632442-18632461 None:intergenic 15.0%
!! AATGAAACAAACAAGAAAAT+AGG + chr4_3:18631745-18631764 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG + chr4_3:18631769-18631788 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG + chr4_3:18632006-18632025 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG - chr4_3:18632007-18632026 Msa0633240:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG - chr4_3:18632684-18632703 Msa0633240:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG + chr4_3:18632710-18632729 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG - chr4_3:18633314-18633333 Msa0633240:CDS 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG - chr4_3:18633455-18633474 Msa0633240:five_prime_UTR 20.0%
!!! ATTTTGCATATTAGTTTTGT+AGG - chr4_3:18633553-18633572 Msa0633240:five_prime_UTR 20.0%
!! AATGAAACAAACAAGAAAAT+AGG + chr4_3:18631745-18631764 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG + chr4_3:18631769-18631788 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG + chr4_3:18632006-18632025 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG - chr4_3:18632007-18632026 Msa0633240:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG - chr4_3:18632684-18632703 Msa0633240:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG + chr4_3:18632710-18632729 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG - chr4_3:18633314-18633333 Msa0633240:CDS 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG - chr4_3:18633455-18633474 Msa0633240:five_prime_UTR 20.0%
! TGGTTAATCTCATATACTTA+CGG + chr4_3:18631317-18631336 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG - chr4_3:18631382-18631401 Msa0633240:three_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG - chr4_3:18631530-18631549 Msa0633240:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG - chr4_3:18632174-18632193 Msa0633240:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG - chr4_3:18632309-18632328 Msa0633240:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG - chr4_3:18632310-18632329 Msa0633240:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG - chr4_3:18632474-18632493 Msa0633240:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG - chr4_3:18632615-18632634 Msa0633240:intron 25.0%
!! TTATCTGAAATAGAATCCAT+AGG - chr4_3:18632859-18632878 Msa0633240:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG + chr4_3:18632878-18632897 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG - chr4_3:18632879-18632898 Msa0633240:CDS 25.0%
!!! TTGATGATTTTGACATTGAA+TGG - chr4_3:18633270-18633289 Msa0633240:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG + chr4_3:18633400-18633419 None:intergenic 25.0%
! TGGTTAATCTCATATACTTA+CGG + chr4_3:18631317-18631336 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG - chr4_3:18631382-18631401 Msa0633240:three_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG - chr4_3:18631530-18631549 Msa0633240:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG - chr4_3:18632174-18632193 Msa0633240:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG - chr4_3:18632309-18632328 Msa0633240:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG - chr4_3:18632310-18632329 Msa0633240:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG - chr4_3:18632474-18632493 Msa0633240:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG - chr4_3:18632615-18632634 Msa0633240:intron 25.0%
!! TTATCTGAAATAGAATCCAT+AGG - chr4_3:18632859-18632878 Msa0633240:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG + chr4_3:18632878-18632897 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG - chr4_3:18632879-18632898 Msa0633240:CDS 25.0%
!!! TTGATGATTTTGACATTGAA+TGG - chr4_3:18633270-18633289 Msa0633240:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG + chr4_3:18633400-18633419 None:intergenic 25.0%
GAGATTAACCAACAAGAATT+TGG - chr4_3:18631326-18631345 Msa0633240:three_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG + chr4_3:18631372-18631391 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG - chr4_3:18632226-18632245 Msa0633240:three_prime_UTR 30.0%
CTAGGCCTATTAAAAGATAA+AGG + chr4_3:18632692-18632711 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG - chr4_3:18632895-18632914 Msa0633240:CDS 30.0%
AGAGAGATAATAAACATCCA+TGG - chr4_3:18632987-18633006 Msa0633240:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG + chr4_3:18633007-18633026 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG - chr4_3:18633194-18633213 Msa0633240:intron 30.0%
GAGATTAACCAACAAGAATT+TGG - chr4_3:18631326-18631345 Msa0633240:three_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG + chr4_3:18631372-18631391 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG - chr4_3:18632226-18632245 Msa0633240:three_prime_UTR 30.0%
CTAGGCCTATTAAAAGATAA+AGG + chr4_3:18632692-18632711 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG - chr4_3:18632895-18632914 Msa0633240:CDS 30.0%
AGAGAGATAATAAACATCCA+TGG - chr4_3:18632987-18633006 Msa0633240:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG + chr4_3:18633007-18633026 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG - chr4_3:18633194-18633213 Msa0633240:intron 30.0%
ATTGTGACGATTATCGATGT+CGG - chr4_3:18631417-18631436 Msa0633240:three_prime_UTR 35.0%
AACGCTAATGTTATCACTGT+TGG - chr4_3:18631462-18631481 Msa0633240:three_prime_UTR 35.0%
AATGTTATCACTGTTGGTGT+CGG - chr4_3:18631468-18631487 Msa0633240:intron 35.0%
ACAAGCAGCAAATTCAATCA+AGG + chr4_3:18631698-18631717 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG - chr4_3:18631931-18631950 Msa0633240:intron 35.0%
AGGAATGTGAAAGACAAAAG+TGG + chr4_3:18631986-18632005 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG - chr4_3:18632018-18632037 Msa0633240:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG + chr4_3:18632071-18632090 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG + chr4_3:18632072-18632091 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG - chr4_3:18632071-18632090 Msa0633240:intron 35.0%
AAACGTTGAGAAAGTGAACT+GGG - chr4_3:18632072-18632091 Msa0633240:intron 35.0%
TCTGATTAGAGTTACTCATG+AGG - chr4_3:18632357-18632376 Msa0633240:intron 35.0%
ATCTCAACACACCATATCAT+AGG + chr4_3:18632389-18632408 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG - chr4_3:18632398-18632417 Msa0633240:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG - chr4_3:18632576-18632595 Msa0633240:intron 35.0%
ACTTGCCATAATGAAACTGT+AGG + chr4_3:18632584-18632603 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG + chr4_3:18632816-18632835 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG - chr4_3:18632817-18632836 Msa0633240:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG - chr4_3:18632902-18632921 Msa0633240:CDS 35.0%
TTCATCACATTGGCAATCAT+TGG + chr4_3:18633362-18633381 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG + chr4_3:18633386-18633405 None:intergenic 35.0%
ATTGTGACGATTATCGATGT+CGG - chr4_3:18631417-18631436 Msa0633240:three_prime_UTR 35.0%
AACGCTAATGTTATCACTGT+TGG - chr4_3:18631462-18631481 Msa0633240:three_prime_UTR 35.0%
AATGTTATCACTGTTGGTGT+CGG - chr4_3:18631468-18631487 Msa0633240:intron 35.0%
ACAAGCAGCAAATTCAATCA+AGG + chr4_3:18631698-18631717 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG - chr4_3:18631931-18631950 Msa0633240:intron 35.0%
AGGAATGTGAAAGACAAAAG+TGG + chr4_3:18631986-18632005 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG - chr4_3:18632018-18632037 Msa0633240:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG + chr4_3:18632071-18632090 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG + chr4_3:18632072-18632091 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG - chr4_3:18632071-18632090 Msa0633240:intron 35.0%
AAACGTTGAGAAAGTGAACT+GGG - chr4_3:18632072-18632091 Msa0633240:intron 35.0%
TCTGATTAGAGTTACTCATG+AGG - chr4_3:18632357-18632376 Msa0633240:intron 35.0%
ATCTCAACACACCATATCAT+AGG + chr4_3:18632389-18632408 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG - chr4_3:18632398-18632417 Msa0633240:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG - chr4_3:18632576-18632595 Msa0633240:intron 35.0%
ACTTGCCATAATGAAACTGT+AGG + chr4_3:18632584-18632603 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG + chr4_3:18632816-18632835 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG - chr4_3:18632817-18632836 Msa0633240:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG - chr4_3:18632902-18632921 Msa0633240:CDS 35.0%
TTCATCACATTGGCAATCAT+TGG + chr4_3:18633362-18633381 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG + chr4_3:18633386-18633405 None:intergenic 35.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG - chr4_3:18631249-18631268 Msa0633240:three_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG + chr4_3:18631337-18631356 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG - chr4_3:18631338-18631357 Msa0633240:three_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG + chr4_3:18631355-18631374 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG - chr4_3:18631500-18631519 Msa0633240:intron 40.0%
AGAGATGAACTTGGATCCAA+TGG + chr4_3:18631549-18631568 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG + chr4_3:18631558-18631577 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG - chr4_3:18631894-18631913 Msa0633240:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG + chr4_3:18631939-18631958 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG - chr4_3:18632080-18632099 Msa0633240:intron 40.0%
GAGTTAGACAGAACGATTCA+TGG - chr4_3:18632109-18632128 Msa0633240:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG - chr4_3:18632319-18632338 Msa0633240:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG - chr4_3:18632360-18632379 Msa0633240:intron 40.0%
TGAGGTGGTTTCCTATGATA+TGG - chr4_3:18632375-18632394 Msa0633240:intron 40.0%
TTAGTGTTCTGGTGCAAATC+AGG - chr4_3:18632409-18632428 Msa0633240:exon 40.0%
TGAGTAGGAATGTTGTGTGT+AGG - chr4_3:18632489-18632508 Msa0633240:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG - chr4_3:18632490-18632509 Msa0633240:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG - chr4_3:18632593-18632612 Msa0633240:intron 40.0%
! TGTGACCAAGACTGACTTAA+TGG + chr4_3:18632825-18632844 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG + chr4_3:18633118-18633137 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG + chr4_3:18633133-18633152 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG + chr4_3:18633172-18633191 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG + chr4_3:18633372-18633391 None:intergenic 40.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG - chr4_3:18631249-18631268 Msa0633240:three_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG + chr4_3:18631337-18631356 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG - chr4_3:18631338-18631357 Msa0633240:three_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG + chr4_3:18631355-18631374 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG - chr4_3:18631500-18631519 Msa0633240:intron 40.0%
AGAGATGAACTTGGATCCAA+TGG + chr4_3:18631549-18631568 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG + chr4_3:18631558-18631577 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG - chr4_3:18631894-18631913 Msa0633240:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG + chr4_3:18631939-18631958 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG - chr4_3:18632080-18632099 Msa0633240:intron 40.0%
GAGTTAGACAGAACGATTCA+TGG - chr4_3:18632109-18632128 Msa0633240:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG - chr4_3:18632319-18632338 Msa0633240:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG - chr4_3:18632360-18632379 Msa0633240:intron 40.0%
TGAGGTGGTTTCCTATGATA+TGG - chr4_3:18632375-18632394 Msa0633240:intron 40.0%
TTAGTGTTCTGGTGCAAATC+AGG - chr4_3:18632409-18632428 Msa0633240:exon 40.0%
TGAGTAGGAATGTTGTGTGT+AGG - chr4_3:18632489-18632508 Msa0633240:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG - chr4_3:18632490-18632509 Msa0633240:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG - chr4_3:18632593-18632612 Msa0633240:intron 40.0%
! TGTGACCAAGACTGACTTAA+TGG + chr4_3:18632825-18632844 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG + chr4_3:18633118-18633137 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG + chr4_3:18633133-18633152 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG + chr4_3:18633172-18633191 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG + chr4_3:18633372-18633391 None:intergenic 40.0%
!! GGTGATTCTTGTGTTTTGGG+AGG - chr4_3:18631248-18631267 Msa0633240:three_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG - chr4_3:18631253-18631272 Msa0633240:three_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG - chr4_3:18631264-18631283 Msa0633240:three_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG - chr4_3:18631265-18631284 Msa0633240:three_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG - chr4_3:18631501-18631520 Msa0633240:intron 45.0%
CTGATGCCGATATGTTGATG+AGG + chr4_3:18631940-18631959 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG - chr4_3:18631938-18631957 Msa0633240:intron 45.0%
ATATCGGCATCAGGTCAACA+TGG - chr4_3:18631947-18631966 Msa0633240:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG - chr4_3:18632085-18632104 Msa0633240:intron 45.0%
GAAACTGTAGGTGCAAAACC+AGG + chr4_3:18632572-18632591 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG - chr4_3:18633122-18633141 Msa0633240:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG - chr4_3:18633123-18633142 Msa0633240:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG - chr4_3:18633333-18633352 Msa0633240:CDS 45.0%
!! GGTGATTCTTGTGTTTTGGG+AGG - chr4_3:18631248-18631267 Msa0633240:three_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG - chr4_3:18631253-18631272 Msa0633240:three_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG - chr4_3:18631264-18631283 Msa0633240:three_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG - chr4_3:18631265-18631284 Msa0633240:three_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG - chr4_3:18631501-18631520 Msa0633240:intron 45.0%
CTGATGCCGATATGTTGATG+AGG + chr4_3:18631940-18631959 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG - chr4_3:18631938-18631957 Msa0633240:intron 45.0%
ATATCGGCATCAGGTCAACA+TGG - chr4_3:18631947-18631966 Msa0633240:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG - chr4_3:18632085-18632104 Msa0633240:intron 45.0%
GAAACTGTAGGTGCAAAACC+AGG + chr4_3:18632572-18632591 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG - chr4_3:18633122-18633141 Msa0633240:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG - chr4_3:18633123-18633142 Msa0633240:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG - chr4_3:18633333-18633352 Msa0633240:CDS 45.0%
CGATGTCGGCGAAGTTAGTT+CGG - chr4_3:18631431-18631450 Msa0633240:three_prime_UTR 50.0%
TCCATTTGCACCTACACTCC+TGG - chr4_3:18632551-18632570 Msa0633240:intron 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG + chr4_3:18632555-18632574 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG + chr4_3:18632564-18632583 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG - chr4_3:18633055-18633074 Msa0633240:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG - chr4_3:18633068-18633087 Msa0633240:intron 50.0%
GCCTAACCATACTGCTGATG+GGG - chr4_3:18633124-18633143 Msa0633240:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG + chr4_3:18633128-18633147 None:intergenic 50.0%
CGATGTCGGCGAAGTTAGTT+CGG - chr4_3:18631431-18631450 Msa0633240:three_prime_UTR 50.0%
TCCATTTGCACCTACACTCC+TGG - chr4_3:18632551-18632570 Msa0633240:intron 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG + chr4_3:18632555-18632574 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG + chr4_3:18632564-18632583 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG - chr4_3:18633055-18633074 Msa0633240:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG - chr4_3:18633068-18633087 Msa0633240:intron 50.0%
GCCTAACCATACTGCTGATG+GGG - chr4_3:18633124-18633143 Msa0633240:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG + chr4_3:18633128-18633147 None:intergenic 50.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG - chr4_3:18631477-18631496 Msa0633240:intron 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG - chr4_3:18631488-18631507 Msa0633240:intron 55.0%
CTCTGGTTGTACTCTCGGGA+AGG - chr4_3:18631505-18631524 Msa0633240:intron 55.0%
GAGCCTGCTACGTCTTCTGA+GGG + chr4_3:18633061-18633080 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG + chr4_3:18633062-18633081 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG - chr4_3:18633067-18633086 Msa0633240:intron 55.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG - chr4_3:18631477-18631496 Msa0633240:intron 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG - chr4_3:18631488-18631507 Msa0633240:intron 55.0%
CTCTGGTTGTACTCTCGGGA+AGG - chr4_3:18631505-18631524 Msa0633240:intron 55.0%
GAGCCTGCTACGTCTTCTGA+GGG + chr4_3:18633061-18633080 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG + chr4_3:18633062-18633081 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG - chr4_3:18633067-18633086 Msa0633240:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 18631247 18633572 18631247 ID=Msa0633240;Name=Msa0633240
chr4_3 mRNA 18631247 18633572 18631247 ID=Msa0633240-mRNA-1;Parent=Msa0633240;Name=Msa0633240-mRNA-1;_AED=0.23;_eAED=0.23;_QI=186|0.5|0.71|0.85|0.66|0.57|7|388|100
chr4_3 exon 18631247 18631483 18631247 ID=Msa0633240-mRNA-1:exon:5110;Parent=Msa0633240-mRNA-1
chr4_3 exon 18632183 18632308 18632183 ID=Msa0633240-mRNA-1:exon:5109;Parent=Msa0633240-mRNA-1
chr4_3 exon 18632389 18632488 18632389 ID=Msa0633240-mRNA-1:exon:5108;Parent=Msa0633240-mRNA-1
chr4_3 exon 18632619 18632623 18632619 ID=Msa0633240-mRNA-1:exon:5107;Parent=Msa0633240-mRNA-1
chr4_3 exon 18632713 18632779 18632713 ID=Msa0633240-mRNA-1:exon:5106;Parent=Msa0633240-mRNA-1
chr4_3 exon 18632860 18632921 18632860 ID=Msa0633240-mRNA-1:exon:5105;Parent=Msa0633240-mRNA-1
chr4_3 exon 18633293 18633572 18633293 ID=Msa0633240-mRNA-1:exon:5104;Parent=Msa0633240-mRNA-1
chr4_3 five_prime_UTR 18633387 18633572 18633387 ID=Msa0633240-mRNA-1:five_prime_utr;Parent=Msa0633240-mRNA-1
chr4_3 CDS 18633293 18633386 18633293 ID=Msa0633240-mRNA-1:cds;Parent=Msa0633240-mRNA-1
chr4_3 CDS 18632860 18632921 18632860 ID=Msa0633240-mRNA-1:cds;Parent=Msa0633240-mRNA-1
chr4_3 CDS 18632713 18632779 18632713 ID=Msa0633240-mRNA-1:cds;Parent=Msa0633240-mRNA-1
chr4_3 CDS 18632619 18632623 18632619 ID=Msa0633240-mRNA-1:cds;Parent=Msa0633240-mRNA-1
chr4_3 CDS 18632414 18632488 18632414 ID=Msa0633240-mRNA-1:cds;Parent=Msa0633240-mRNA-1
chr4_3 three_prime_UTR 18632389 18632413 18632389 ID=Msa0633240-mRNA-1:three_prime_utr;Parent=Msa0633240-mRNA-1
chr4_3 three_prime_UTR 18632183 18632308 18632183 ID=Msa0633240-mRNA-1:three_prime_utr;Parent=Msa0633240-mRNA-1
chr4_3 three_prime_UTR 18631247 18631483 18631247 ID=Msa0633240-mRNA-1:three_prime_utr;Parent=Msa0633240-mRNA-1
Gene Sequence

>Msa0633240

ATGTCGGCGAAGTTAGTTCGGAAATATTCAACGCTAATGTTATCACTGTTGGTGTCGGTGTTGCTGGTATTGCTCTGGTTGTACTCTCGGGAAGTTATCAACATCTTCGTTGGCATTTGTGTATCTGCATTTCCCTCATCAACATATCGGCATCAGGTGAACAAGCTTAAAAGAGAACTTGAAACCCAAAACGTTGAGAAAGTGAACTGGGAAACTTGGATAGGTTGTGGTTCTCTTGGTTGCAGTTTCTGCAGTCTGATTAGAGTTACTCATGAGGTGGTTTCCTATGATATGGTGTGTTGA
Protein sequence

>Msa0633240

MSAKLVRKYSTLMLSLLVSVLLVLLWLYSREVINIFVGICVSAFPSSTYRHQVNKLKRELETQNVEKVNWETWIGCGSLGCSFCSLIRVTHEVVSYDMVC*