AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1013100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTAACCAACAAGAATT+TGG 0.215065 7_1:+19206182 Msa1013100:five_prime_UTR
AGTTCACTTTCTCAACGTTT+TGG 0.264629 7_1:-19206925 None:intergenic
TGAAGGTGATTCTTGTGTTT+TGG 0.303850 7_1:+19206100 None:intergenic
TTGCACCTACAGTTTCATTA+TGG 0.321692 7_1:+19207432 Msa1013100:three_prime_UTR
ACTGTTGGTGTCGGTGTTGC+TGG 0.340769 7_1:+19206333 Msa1013100:CDS
TTAGTGTTCTGGTGCAAATC+AGG 0.390681 7_1:+19207265 Msa1013100:three_prime_UTR
TGTGTTGAGATTTAGTGTTC+TGG 0.393710 7_1:+19207254 Msa1013100:exon
CGATGTCGGCGAAGTTAGTT+CGG 0.393719 7_1:+19206287 Msa1013100:exon
TTCATCACATTGGCAATCAT+TGG 0.396788 7_1:-19208215 None:intergenic
TCCATTTGCACCTACACTCC+TGG 0.409884 7_1:+19207407 Msa1013100:three_prime_UTR
GAAATGAAACATTGTTCTAA+AGG 0.420124 7_1:+19207471 Msa1013100:three_prime_UTR
AGAAAGTGAACTGGGAAACT+TGG 0.423349 7_1:+19206936 Msa1013100:CDS
TATTGCTCTGGTTGTACTCT+CGG 0.424030 7_1:+19206356 Msa1013100:CDS
CTTAAGAAGTGTAATTATGA+AGG 0.426449 7_1:+19206238 Msa1013100:five_prime_UTR
ACCAGGAGTGTAGGTGCAAA+TGG 0.432206 7_1:-19207408 None:intergenic
CGGTGTTGCTGGTATTGCTC+TGG 0.450550 7_1:+19206344 Msa1013100:CDS
TGGTTAATCTCATATACTTA+CGG 0.460145 7_1:-19206170 None:intergenic
TGAGGTGGTTTCCTATGATA+TGG 0.460675 7_1:+19207231 Msa1013100:CDS
TTATGGCAAGTGACAAGATC+AGG 0.462078 7_1:+19207449 Msa1013100:three_prime_UTR
ATCTCAACACACCATATCAT+AGG 0.464654 7_1:-19207242 None:intergenic
CATTTCCCTCATCAACATAT+CGG 0.470848 7_1:+19206787 Msa1013100:CDS
AAAACGTTGAGAAAGTGAAC+TGG 0.473660 7_1:+19206927 Msa1013100:CDS
AACGCTAATGTTATCACTGT+TGG 0.479387 7_1:+19206318 Msa1013100:CDS
CTCATCAACATATCGGCATC+AGG 0.481451 7_1:+19206794 Msa1013100:CDS
GCAGTTATCAACATCTTCGT+TGG 0.491361 7_1:+19206750 Msa1013100:intron
ACTTCTTAAGCATCTTTACA+AGG 0.508307 7_1:-19206225 None:intergenic
GTTGGATGCCAAATTCTTGT+TGG 0.510438 7_1:-19206190 None:intergenic
CAGGCATAAGCAAGTGATGT+AGG 0.512448 7_1:+19208189 Msa1013100:intron
ACAAGGATCTTAAGCCATGT+TGG 0.517216 7_1:-19206208 None:intergenic
ATTGCTCTGGTTGTACTCTC+GGG 0.525362 7_1:+19206357 Msa1013100:CDS
CAAGAATTTGGCATCCAACA+TGG 0.531190 7_1:+19206194 Msa1013100:five_prime_UTR
TTCTTGAGGCTTCATCACAT+TGG 0.566647 7_1:-19208225 None:intergenic
CTCTGGTTGTACTCTCGGGA+AGG 0.577901 7_1:+19206361 Msa1013100:CDS
GATTAGAGTTACTCATGAGG+TGG 0.581584 7_1:+19207216 Msa1013100:CDS
AGGTGCAAAACCAGGAGTGT+AGG 0.604566 7_1:-19207417 None:intergenic
AATGTTATCACTGTTGGTGT+CGG 0.605324 7_1:+19206324 Msa1013100:CDS
AAACGTTGAGAAAGTGAACT+GGG 0.630136 7_1:+19206928 Msa1013100:CDS
GAAACTGTAGGTGCAAAACC+AGG 0.636631 7_1:-19207425 None:intergenic
ATTGTGACGATTATCGATGT+CGG 0.639449 7_1:+19206273 Msa1013100:exon
CTGATGCCGATATGTTGATG+AGG 0.659856 7_1:-19206793 None:intergenic
GTGAACTGGGAAACTTGGAT+AGG 0.670748 7_1:+19206941 Msa1013100:CDS
GTATTCAGGAGATATTCTTG+AGG 0.672009 7_1:-19208239 None:intergenic
TGATGCCGATATGTTGATGA+GGG 0.679239 7_1:-19206792 None:intergenic
ACTTGCCATAATGAAACTGT+AGG 0.704994 7_1:-19207437 None:intergenic
TCTGATTAGAGTTACTCATG+AGG 0.713055 7_1:+19207213 Msa1013100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGATTGATTTTATATTT+GGG + chr7_1:19206713-19206732 Msa1013100:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG + chr7_1:19207312-19207331 Msa1013100:intron 10.0%
!!! TATAGATTGATTTTATATTT+GGG + chr7_1:19206713-19206732 Msa1013100:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG + chr7_1:19207312-19207331 Msa1013100:intron 10.0%
!!! AAGTTTTGATTTGATTTATT+TGG + chr7_1:19206441-19206460 Msa1013100:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG + chr7_1:19206712-19206731 Msa1013100:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG - chr7_1:19207298-19207317 None:intergenic 15.0%
!!! AAGTTTTGATTTGATTTATT+TGG + chr7_1:19206441-19206460 Msa1013100:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG + chr7_1:19206712-19206731 Msa1013100:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG - chr7_1:19207298-19207317 None:intergenic 15.0%
!! AATGAAACAAACAAGAAAAT+AGG - chr7_1:19206601-19206620 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG - chr7_1:19206625-19206644 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG - chr7_1:19206862-19206881 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG + chr7_1:19206863-19206882 Msa1013100:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG + chr7_1:19207540-19207559 Msa1013100:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG - chr7_1:19207566-19207585 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG + chr7_1:19208170-19208189 Msa1013100:intron 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG + chr7_1:19208311-19208330 Msa1013100:three_prime_UTR 20.0%
!!! ATTTTGCATATTAGTTTTGT+AGG + chr7_1:19208409-19208428 Msa1013100:three_prime_UTR 20.0%
!! AATGAAACAAACAAGAAAAT+AGG - chr7_1:19206601-19206620 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG - chr7_1:19206625-19206644 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG - chr7_1:19206862-19206881 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG + chr7_1:19206863-19206882 Msa1013100:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG + chr7_1:19207540-19207559 Msa1013100:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG - chr7_1:19207566-19207585 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG + chr7_1:19208170-19208189 Msa1013100:intron 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG + chr7_1:19208311-19208330 Msa1013100:three_prime_UTR 20.0%
! TGGTTAATCTCATATACTTA+CGG - chr7_1:19206173-19206192 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG + chr7_1:19206238-19206257 Msa1013100:five_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG + chr7_1:19206386-19206405 Msa1013100:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG + chr7_1:19207030-19207049 Msa1013100:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG + chr7_1:19207165-19207184 Msa1013100:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG + chr7_1:19207166-19207185 Msa1013100:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG + chr7_1:19207330-19207349 Msa1013100:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG + chr7_1:19207471-19207490 Msa1013100:three_prime_UTR 25.0%
!! TTATCTGAAATAGAATCCAT+AGG + chr7_1:19207715-19207734 Msa1013100:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG - chr7_1:19207734-19207753 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG + chr7_1:19207735-19207754 Msa1013100:intron 25.0%
!!! TTGATGATTTTGACATTGAA+TGG + chr7_1:19208126-19208145 Msa1013100:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG - chr7_1:19208256-19208275 None:intergenic 25.0%
! TGGTTAATCTCATATACTTA+CGG - chr7_1:19206173-19206192 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG + chr7_1:19206238-19206257 Msa1013100:five_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG + chr7_1:19206386-19206405 Msa1013100:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG + chr7_1:19207030-19207049 Msa1013100:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG + chr7_1:19207165-19207184 Msa1013100:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG + chr7_1:19207166-19207185 Msa1013100:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG + chr7_1:19207330-19207349 Msa1013100:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG + chr7_1:19207471-19207490 Msa1013100:three_prime_UTR 25.0%
!! TTATCTGAAATAGAATCCAT+AGG + chr7_1:19207715-19207734 Msa1013100:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG - chr7_1:19207734-19207753 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG + chr7_1:19207735-19207754 Msa1013100:intron 25.0%
!!! TTGATGATTTTGACATTGAA+TGG + chr7_1:19208126-19208145 Msa1013100:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG - chr7_1:19208256-19208275 None:intergenic 25.0%
GAGATTAACCAACAAGAATT+TGG + chr7_1:19206182-19206201 Msa1013100:five_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG - chr7_1:19206228-19206247 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG + chr7_1:19207082-19207101 Msa1013100:intron 30.0%
CTAGGCCTATTAAAAGATAA+AGG - chr7_1:19207548-19207567 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG + chr7_1:19207751-19207770 Msa1013100:intron 30.0%
AGAGAGATAATAAACATCCA+TGG + chr7_1:19207843-19207862 Msa1013100:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG - chr7_1:19207863-19207882 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG + chr7_1:19208050-19208069 Msa1013100:intron 30.0%
GAGATTAACCAACAAGAATT+TGG + chr7_1:19206182-19206201 Msa1013100:five_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG - chr7_1:19206228-19206247 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG + chr7_1:19207082-19207101 Msa1013100:intron 30.0%
CTAGGCCTATTAAAAGATAA+AGG - chr7_1:19207548-19207567 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG + chr7_1:19207751-19207770 Msa1013100:intron 30.0%
AGAGAGATAATAAACATCCA+TGG + chr7_1:19207843-19207862 Msa1013100:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG - chr7_1:19207863-19207882 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG + chr7_1:19208050-19208069 Msa1013100:intron 30.0%
ATTGTGACGATTATCGATGT+CGG + chr7_1:19206273-19206292 Msa1013100:exon 35.0%
AACGCTAATGTTATCACTGT+TGG + chr7_1:19206318-19206337 Msa1013100:CDS 35.0%
AATGTTATCACTGTTGGTGT+CGG + chr7_1:19206324-19206343 Msa1013100:CDS 35.0%
ACAAGCAGCAAATTCAATCA+AGG - chr7_1:19206554-19206573 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG + chr7_1:19206787-19206806 Msa1013100:CDS 35.0%
AGGAATGTGAAAGACAAAAG+TGG - chr7_1:19206842-19206861 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG + chr7_1:19206874-19206893 Msa1013100:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG - chr7_1:19206927-19206946 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG - chr7_1:19206928-19206947 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG + chr7_1:19206927-19206946 Msa1013100:CDS 35.0%
AAACGTTGAGAAAGTGAACT+GGG + chr7_1:19206928-19206947 Msa1013100:CDS 35.0%
TCTGATTAGAGTTACTCATG+AGG + chr7_1:19207213-19207232 Msa1013100:CDS 35.0%
ATCTCAACACACCATATCAT+AGG - chr7_1:19207245-19207264 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG + chr7_1:19207254-19207273 Msa1013100:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG + chr7_1:19207432-19207451 Msa1013100:three_prime_UTR 35.0%
ACTTGCCATAATGAAACTGT+AGG - chr7_1:19207440-19207459 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG - chr7_1:19207672-19207691 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG + chr7_1:19207673-19207692 Msa1013100:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG + chr7_1:19207758-19207777 Msa1013100:intron 35.0%
TTCATCACATTGGCAATCAT+TGG - chr7_1:19208218-19208237 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG - chr7_1:19208242-19208261 None:intergenic 35.0%
ATTGTGACGATTATCGATGT+CGG + chr7_1:19206273-19206292 Msa1013100:exon 35.0%
AACGCTAATGTTATCACTGT+TGG + chr7_1:19206318-19206337 Msa1013100:CDS 35.0%
AATGTTATCACTGTTGGTGT+CGG + chr7_1:19206324-19206343 Msa1013100:CDS 35.0%
ACAAGCAGCAAATTCAATCA+AGG - chr7_1:19206554-19206573 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG + chr7_1:19206787-19206806 Msa1013100:CDS 35.0%
AGGAATGTGAAAGACAAAAG+TGG - chr7_1:19206842-19206861 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG + chr7_1:19206874-19206893 Msa1013100:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG - chr7_1:19206927-19206946 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG - chr7_1:19206928-19206947 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG + chr7_1:19206927-19206946 Msa1013100:CDS 35.0%
AAACGTTGAGAAAGTGAACT+GGG + chr7_1:19206928-19206947 Msa1013100:CDS 35.0%
TCTGATTAGAGTTACTCATG+AGG + chr7_1:19207213-19207232 Msa1013100:CDS 35.0%
ATCTCAACACACCATATCAT+AGG - chr7_1:19207245-19207264 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG + chr7_1:19207254-19207273 Msa1013100:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG + chr7_1:19207432-19207451 Msa1013100:three_prime_UTR 35.0%
ACTTGCCATAATGAAACTGT+AGG - chr7_1:19207440-19207459 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG - chr7_1:19207672-19207691 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG + chr7_1:19207673-19207692 Msa1013100:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG + chr7_1:19207758-19207777 Msa1013100:intron 35.0%
TTCATCACATTGGCAATCAT+TGG - chr7_1:19208218-19208237 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG - chr7_1:19208242-19208261 None:intergenic 35.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG + chr7_1:19206105-19206124 Msa1013100:five_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG - chr7_1:19206193-19206212 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG + chr7_1:19206194-19206213 Msa1013100:five_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG - chr7_1:19206211-19206230 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG + chr7_1:19206356-19206375 Msa1013100:CDS 40.0%
AGAGATGAACTTGGATCCAA+TGG - chr7_1:19206405-19206424 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG - chr7_1:19206414-19206433 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG + chr7_1:19206750-19206769 Msa1013100:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG - chr7_1:19206795-19206814 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG + chr7_1:19206936-19206955 Msa1013100:CDS 40.0%
GAGTTAGACAGAACGATTCA+TGG + chr7_1:19206965-19206984 Msa1013100:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG + chr7_1:19207175-19207194 Msa1013100:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG + chr7_1:19207216-19207235 Msa1013100:CDS 40.0%
TGAGGTGGTTTCCTATGATA+TGG + chr7_1:19207231-19207250 Msa1013100:CDS 40.0%
TTAGTGTTCTGGTGCAAATC+AGG + chr7_1:19207265-19207284 Msa1013100:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG + chr7_1:19207345-19207364 Msa1013100:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG + chr7_1:19207346-19207365 Msa1013100:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG + chr7_1:19207449-19207468 Msa1013100:three_prime_UTR 40.0%
! TGTGACCAAGACTGACTTAA+TGG - chr7_1:19207681-19207700 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG - chr7_1:19207974-19207993 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG - chr7_1:19207989-19208008 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG - chr7_1:19208028-19208047 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG - chr7_1:19208228-19208247 None:intergenic 40.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG + chr7_1:19206105-19206124 Msa1013100:five_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG - chr7_1:19206193-19206212 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG + chr7_1:19206194-19206213 Msa1013100:five_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG - chr7_1:19206211-19206230 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG + chr7_1:19206356-19206375 Msa1013100:CDS 40.0%
AGAGATGAACTTGGATCCAA+TGG - chr7_1:19206405-19206424 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG - chr7_1:19206414-19206433 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG + chr7_1:19206750-19206769 Msa1013100:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG - chr7_1:19206795-19206814 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG + chr7_1:19206936-19206955 Msa1013100:CDS 40.0%
GAGTTAGACAGAACGATTCA+TGG + chr7_1:19206965-19206984 Msa1013100:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG + chr7_1:19207175-19207194 Msa1013100:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG + chr7_1:19207216-19207235 Msa1013100:CDS 40.0%
TGAGGTGGTTTCCTATGATA+TGG + chr7_1:19207231-19207250 Msa1013100:CDS 40.0%
TTAGTGTTCTGGTGCAAATC+AGG + chr7_1:19207265-19207284 Msa1013100:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG + chr7_1:19207345-19207364 Msa1013100:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG + chr7_1:19207346-19207365 Msa1013100:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG + chr7_1:19207449-19207468 Msa1013100:three_prime_UTR 40.0%
! TGTGACCAAGACTGACTTAA+TGG - chr7_1:19207681-19207700 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG - chr7_1:19207974-19207993 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG - chr7_1:19207989-19208008 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG - chr7_1:19208028-19208047 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG - chr7_1:19208228-19208247 None:intergenic 40.0%
!! GGTGATTCTTGTGTTTTGGG+AGG + chr7_1:19206104-19206123 Msa1013100:five_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG + chr7_1:19206109-19206128 Msa1013100:five_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG + chr7_1:19206120-19206139 Msa1013100:five_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG + chr7_1:19206121-19206140 Msa1013100:five_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG + chr7_1:19206357-19206376 Msa1013100:CDS 45.0%
CTGATGCCGATATGTTGATG+AGG - chr7_1:19206796-19206815 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG + chr7_1:19206794-19206813 Msa1013100:CDS 45.0%
ATATCGGCATCAGGTCAACA+TGG + chr7_1:19206803-19206822 Msa1013100:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG + chr7_1:19206941-19206960 Msa1013100:CDS 45.0%
GAAACTGTAGGTGCAAAACC+AGG - chr7_1:19207428-19207447 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG + chr7_1:19207978-19207997 Msa1013100:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG + chr7_1:19207979-19207998 Msa1013100:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG + chr7_1:19208189-19208208 Msa1013100:intron 45.0%
!! GGTGATTCTTGTGTTTTGGG+AGG + chr7_1:19206104-19206123 Msa1013100:five_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG + chr7_1:19206109-19206128 Msa1013100:five_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG + chr7_1:19206120-19206139 Msa1013100:five_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG + chr7_1:19206121-19206140 Msa1013100:five_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG + chr7_1:19206357-19206376 Msa1013100:CDS 45.0%
CTGATGCCGATATGTTGATG+AGG - chr7_1:19206796-19206815 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG + chr7_1:19206794-19206813 Msa1013100:CDS 45.0%
ATATCGGCATCAGGTCAACA+TGG + chr7_1:19206803-19206822 Msa1013100:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG + chr7_1:19206941-19206960 Msa1013100:CDS 45.0%
GAAACTGTAGGTGCAAAACC+AGG - chr7_1:19207428-19207447 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG + chr7_1:19207978-19207997 Msa1013100:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG + chr7_1:19207979-19207998 Msa1013100:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG + chr7_1:19208189-19208208 Msa1013100:intron 45.0%
CGATGTCGGCGAAGTTAGTT+CGG + chr7_1:19206287-19206306 Msa1013100:exon 50.0%
TCCATTTGCACCTACACTCC+TGG + chr7_1:19207407-19207426 Msa1013100:three_prime_UTR 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG - chr7_1:19207411-19207430 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG - chr7_1:19207420-19207439 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG + chr7_1:19207911-19207930 Msa1013100:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG + chr7_1:19207924-19207943 Msa1013100:intron 50.0%
GCCTAACCATACTGCTGATG+GGG + chr7_1:19207980-19207999 Msa1013100:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG - chr7_1:19207984-19208003 None:intergenic 50.0%
CGATGTCGGCGAAGTTAGTT+CGG + chr7_1:19206287-19206306 Msa1013100:exon 50.0%
TCCATTTGCACCTACACTCC+TGG + chr7_1:19207407-19207426 Msa1013100:three_prime_UTR 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG - chr7_1:19207411-19207430 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG - chr7_1:19207420-19207439 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG + chr7_1:19207911-19207930 Msa1013100:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG + chr7_1:19207924-19207943 Msa1013100:intron 50.0%
GCCTAACCATACTGCTGATG+GGG + chr7_1:19207980-19207999 Msa1013100:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG - chr7_1:19207984-19208003 None:intergenic 50.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG + chr7_1:19206333-19206352 Msa1013100:CDS 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG + chr7_1:19206344-19206363 Msa1013100:CDS 55.0%
CTCTGGTTGTACTCTCGGGA+AGG + chr7_1:19206361-19206380 Msa1013100:CDS 55.0%
GAGCCTGCTACGTCTTCTGA+GGG - chr7_1:19207917-19207936 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG - chr7_1:19207918-19207937 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG + chr7_1:19207923-19207942 Msa1013100:intron 55.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG + chr7_1:19206333-19206352 Msa1013100:CDS 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG + chr7_1:19206344-19206363 Msa1013100:CDS 55.0%
CTCTGGTTGTACTCTCGGGA+AGG + chr7_1:19206361-19206380 Msa1013100:CDS 55.0%
GAGCCTGCTACGTCTTCTGA+GGG - chr7_1:19207917-19207936 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG - chr7_1:19207918-19207937 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG + chr7_1:19207923-19207942 Msa1013100:intron 55.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 19206103 19208428 19206103 ID=Msa1013100;Name=Msa1013100
chr7_1 mRNA 19206103 19208428 19206103 ID=Msa1013100-mRNA-1;Parent=Msa1013100;Name=Msa1013100-mRNA-1;_AED=0.23;_eAED=0.23;_QI=186|0.5|0.71|0.85|0.66|0.57|7|388|100
chr7_1 exon 19206103 19206382 19206103 ID=Msa1013100-mRNA-1:exon:4702;Parent=Msa1013100-mRNA-1
chr7_1 exon 19206754 19206815 19206754 ID=Msa1013100-mRNA-1:exon:4703;Parent=Msa1013100-mRNA-1
chr7_1 exon 19206896 19206962 19206896 ID=Msa1013100-mRNA-1:exon:4704;Parent=Msa1013100-mRNA-1
chr7_1 exon 19207052 19207056 19207052 ID=Msa1013100-mRNA-1:exon:4705;Parent=Msa1013100-mRNA-1
chr7_1 exon 19207187 19207286 19207187 ID=Msa1013100-mRNA-1:exon:4706;Parent=Msa1013100-mRNA-1
chr7_1 exon 19207367 19207492 19207367 ID=Msa1013100-mRNA-1:exon:4707;Parent=Msa1013100-mRNA-1
chr7_1 exon 19208192 19208428 19208192 ID=Msa1013100-mRNA-1:exon:4708;Parent=Msa1013100-mRNA-1
chr7_1 five_prime_UTR 19206103 19206288 19206103 ID=Msa1013100-mRNA-1:five_prime_utr;Parent=Msa1013100-mRNA-1
chr7_1 CDS 19206289 19206382 19206289 ID=Msa1013100-mRNA-1:cds;Parent=Msa1013100-mRNA-1
chr7_1 CDS 19206754 19206815 19206754 ID=Msa1013100-mRNA-1:cds;Parent=Msa1013100-mRNA-1
chr7_1 CDS 19206896 19206962 19206896 ID=Msa1013100-mRNA-1:cds;Parent=Msa1013100-mRNA-1
chr7_1 CDS 19207052 19207056 19207052 ID=Msa1013100-mRNA-1:cds;Parent=Msa1013100-mRNA-1
chr7_1 CDS 19207187 19207261 19207187 ID=Msa1013100-mRNA-1:cds;Parent=Msa1013100-mRNA-1
chr7_1 three_prime_UTR 19207262 19207286 19207262 ID=Msa1013100-mRNA-1:three_prime_utr;Parent=Msa1013100-mRNA-1
chr7_1 three_prime_UTR 19207367 19207492 19207367 ID=Msa1013100-mRNA-1:three_prime_utr;Parent=Msa1013100-mRNA-1
chr7_1 three_prime_UTR 19208192 19208428 19208192 ID=Msa1013100-mRNA-1:three_prime_utr;Parent=Msa1013100-mRNA-1
Gene Sequence

>Msa1013100

ATGTCGGCGAAGTTAGTTCGGAAATATTCAACGCTAATGTTATCACTGTTGGTGTCGGTGTTGCTGGTATTGCTCTGGTTGTACTCTCGGGAAGTTATCAACATCTTCGTTGGCATTTGTGTATCTGCATTTCCCTCATCAACATATCGGCATCAGGTGAACAAGCTTAAAAGAGAACTTGAAACCCAAAACGTTGAGAAAGTGAACTGGGAAACTTGGATAGGTTGTGGTTCTCTTGGTTGCAGTTTCTGCAGTCTGATTAGAGTTACTCATGAGGTGGTTTCCTATGATATGGTGTGTTGA
Protein sequence

>Msa1013100

MSAKLVRKYSTLMLSLLVSVLLVLLWLYSREVINIFVGICVSAFPSSTYRHQVNKLKRELETQNVEKVNWETWIGCGSLGCSFCSLIRVTHEVVSYDMVC*