AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0140620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTAACCAACAAGAATT+TGG 0.215065 1_3:-74846750 Msa0140620:five_prime_UTR
AGTTCACTTTCTCAACGTTT+TGG 0.264629 1_3:+74846007 Msa0140610:intron
TGAAGGTGATTCTTGTGTTT+TGG 0.303850 1_3:-74846832 Msa0140620:five_prime_UTR
TTGCACCTACAGTTTCATTA+TGG 0.321692 1_3:-74845500 Msa0140620:three_prime_UTR
ACTGTTGGTGTCGGTGTTGC+TGG 0.340769 1_3:-74846599 Msa0140620:CDS
TTAGTGTTCTGGTGCAAATC+AGG 0.390681 1_3:-74845667 Msa0140620:intron
TGTGTTGAGATTTAGTGTTC+TGG 0.393710 1_3:-74845678 Msa0140620:three_prime_UTR
CGATGTCGGCGAAGTTAGTT+CGG 0.393719 1_3:-74846645 Msa0140620:CDS
TTCATCACATTGGCAATCAT+TGG 0.396788 1_3:+74844717 Msa0140610:intron
TCCATTTGCACCTACACTCC+TGG 0.409884 1_3:-74845525 Msa0140620:three_prime_UTR
GAAATGAAACATTGTTCTAA+AGG 0.420124 1_3:-74845461 Msa0140620:intron
AGAAAGTGAACTGGGAAACT+TGG 0.423349 1_3:-74845996 Msa0140620:CDS
TATTGCTCTGGTTGTACTCT+CGG 0.424030 1_3:-74846576 Msa0140620:CDS
CTTAAGAAGTGTAATTATGA+AGG 0.426449 1_3:-74846694 Msa0140620:five_prime_UTR
ACCAGGAGTGTAGGTGCAAA+TGG 0.432206 1_3:+74845524 Msa0140610:intron
CGGTGTTGCTGGTATTGCTC+TGG 0.450550 1_3:-74846588 Msa0140620:CDS
TGGTTAATCTCATATACTTA+CGG 0.460145 1_3:+74846762 Msa0140610:intron
TGAGGTGGTTTCCTATGATA+TGG 0.460675 1_3:-74845701 Msa0140620:three_prime_UTR
TTATGGCAAGTGACAAGATC+AGG 0.462078 1_3:-74845483 Msa0140620:three_prime_UTR
ATCTCAACACACCATATCAT+AGG 0.464654 1_3:+74845690 Msa0140610:intron
CATTTCCCTCATCAACATAT+CGG 0.470848 1_3:-74846145 Msa0140620:CDS
AAAACGTTGAGAAAGTGAAC+TGG 0.473660 1_3:-74846005 Msa0140620:CDS
AACGCTAATGTTATCACTGT+TGG 0.479387 1_3:-74846614 Msa0140620:CDS
CTCATCAACATATCGGCATC+AGG 0.481451 1_3:-74846138 Msa0140620:intron
GCAGTTATCAACATCTTCGT+TGG 0.491361 1_3:-74846182 Msa0140620:intron
ACTTCTTAAGCATCTTTACA+AGG 0.508307 1_3:+74846707 Msa0140610:intron
GTTGGATGCCAAATTCTTGT+TGG 0.510438 1_3:+74846742 Msa0140610:intron
CAGGCATAAGCAAGTGATGT+AGG 0.512448 1_3:-74844743 Msa0140620:three_prime_UTR
ACAAGGATCTTAAGCCATGT+TGG 0.517216 1_3:+74846724 Msa0140610:intron
ATTGCTCTGGTTGTACTCTC+GGG 0.525362 1_3:-74846575 Msa0140620:CDS
CAAGAATTTGGCATCCAACA+TGG 0.531190 1_3:-74846738 Msa0140620:five_prime_UTR
TTCTTGAGGCTTCATCACAT+TGG 0.566647 1_3:+74844707 Msa0140610:intron
CTCTGGTTGTACTCTCGGGA+AGG 0.577901 1_3:-74846571 Msa0140620:intron
GATTAGAGTTACTCATGAGG+TGG 0.581584 1_3:-74845716 Msa0140620:exon
AGGTGCAAAACCAGGAGTGT+AGG 0.604566 1_3:+74845515 Msa0140610:intron
AATGTTATCACTGTTGGTGT+CGG 0.605324 1_3:-74846608 Msa0140620:CDS
AAACGTTGAGAAAGTGAACT+GGG 0.630136 1_3:-74846004 Msa0140620:CDS
GAAACTGTAGGTGCAAAACC+AGG 0.636631 1_3:+74845507 Msa0140610:intron
ATTGTGACGATTATCGATGT+CGG 0.639449 1_3:-74846659 Msa0140620:exon
CTGATGCCGATATGTTGATG+AGG 0.659856 1_3:+74846139 Msa0140610:intron
GTGAACTGGGAAACTTGGAT+AGG 0.670748 1_3:-74845991 Msa0140620:intron
GTATTCAGGAGATATTCTTG+AGG 0.672009 1_3:+74844693 Msa0140610:intron
TGATGCCGATATGTTGATGA+GGG 0.679239 1_3:+74846140 Msa0140610:intron
ACTTGCCATAATGAAACTGT+AGG 0.704994 1_3:+74845495 Msa0140610:intron
TCTGATTAGAGTTACTCATG+AGG 0.713055 1_3:-74845719 Msa0140620:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGATTGATTTTATATTT+GGG - chr1_3:74845136-74845155 Msa0140620:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG - chr1_3:74845735-74845754 Msa0140620:CDS 10.0%
!!! TATAGATTGATTTTATATTT+GGG - chr1_3:74845136-74845155 Msa0140620:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG - chr1_3:74845735-74845754 Msa0140620:CDS 10.0%
!!! AAGTTTTGATTTGATTTATT+TGG - chr1_3:74844864-74844883 Msa0140620:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG - chr1_3:74845135-74845154 Msa0140620:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG + chr1_3:74845721-74845740 Msa0140610:intron 15.0%
!!! AAGTTTTGATTTGATTTATT+TGG - chr1_3:74844864-74844883 Msa0140620:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG - chr1_3:74845135-74845154 Msa0140620:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG + chr1_3:74845721-74845740 Msa0140610:intron 15.0%
!! AATGAAACAAACAAGAAAAT+AGG + chr1_3:74845024-74845043 Msa0140610:intron 20.0%
!! TTACATATAAACAAAACACA+CGG + chr1_3:74845048-74845067 Msa0140610:intron 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG + chr1_3:74845285-74845304 Msa0140610:intron 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG - chr1_3:74845286-74845305 Msa0140620:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG - chr1_3:74845963-74845982 Msa0140620:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG + chr1_3:74845989-74846008 Msa0140610:intron 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG - chr1_3:74846593-74846612 Msa0140620:CDS 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG - chr1_3:74846734-74846753 Msa0140620:five_prime_UTR 20.0%
!!! ATTTTGCATATTAGTTTTGT+AGG - chr1_3:74846832-74846851 Msa0140620:five_prime_UTR 20.0%
!! AATGAAACAAACAAGAAAAT+AGG + chr1_3:74845024-74845043 Msa0140610:intron 20.0%
!! TTACATATAAACAAAACACA+CGG + chr1_3:74845048-74845067 Msa0140610:intron 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG + chr1_3:74845285-74845304 Msa0140610:intron 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG - chr1_3:74845286-74845305 Msa0140620:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG - chr1_3:74845963-74845982 Msa0140620:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG + chr1_3:74845989-74846008 Msa0140610:intron 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG - chr1_3:74846593-74846612 Msa0140620:CDS 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG - chr1_3:74846734-74846753 Msa0140620:five_prime_UTR 20.0%
! TGGTTAATCTCATATACTTA+CGG + chr1_3:74844596-74844615 Msa0140610:intron 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG - chr1_3:74844661-74844680 Msa0140620:three_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG - chr1_3:74844809-74844828 Msa0140620:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG - chr1_3:74845453-74845472 Msa0140620:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG - chr1_3:74845588-74845607 Msa0140620:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG - chr1_3:74845589-74845608 Msa0140620:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG - chr1_3:74845753-74845772 Msa0140620:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG - chr1_3:74845894-74845913 Msa0140620:intron 25.0%
!! TTATCTGAAATAGAATCCAT+AGG - chr1_3:74846138-74846157 Msa0140620:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG + chr1_3:74846157-74846176 Msa0140610:intron 25.0%
! AGGAATTAAGAATGTCTATT+TGG - chr1_3:74846158-74846177 Msa0140620:CDS 25.0%
!!! TTGATGATTTTGACATTGAA+TGG - chr1_3:74846549-74846568 Msa0140620:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG + chr1_3:74846679-74846698 Msa0140610:intron 25.0%
! TGGTTAATCTCATATACTTA+CGG + chr1_3:74844596-74844615 Msa0140610:intron 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG - chr1_3:74844661-74844680 Msa0140620:three_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG - chr1_3:74844809-74844828 Msa0140620:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG - chr1_3:74845453-74845472 Msa0140620:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG - chr1_3:74845588-74845607 Msa0140620:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG - chr1_3:74845589-74845608 Msa0140620:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG - chr1_3:74845753-74845772 Msa0140620:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG - chr1_3:74845894-74845913 Msa0140620:intron 25.0%
!! TTATCTGAAATAGAATCCAT+AGG - chr1_3:74846138-74846157 Msa0140620:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG + chr1_3:74846157-74846176 Msa0140610:intron 25.0%
! AGGAATTAAGAATGTCTATT+TGG - chr1_3:74846158-74846177 Msa0140620:CDS 25.0%
!!! TTGATGATTTTGACATTGAA+TGG - chr1_3:74846549-74846568 Msa0140620:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG + chr1_3:74846679-74846698 Msa0140610:intron 25.0%
GAGATTAACCAACAAGAATT+TGG - chr1_3:74844605-74844624 Msa0140620:three_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG + chr1_3:74844651-74844670 Msa0140610:intron 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG - chr1_3:74845505-74845524 Msa0140620:three_prime_UTR 30.0%
CTAGGCCTATTAAAAGATAA+AGG + chr1_3:74845971-74845990 Msa0140610:intron 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG - chr1_3:74846174-74846193 Msa0140620:CDS 30.0%
AGAGAGATAATAAACATCCA+TGG - chr1_3:74846266-74846285 Msa0140620:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG + chr1_3:74846286-74846305 Msa0140610:intron 30.0%
TTTGTCAAATGTGATGTAGA+AGG - chr1_3:74846473-74846492 Msa0140620:intron 30.0%
GAGATTAACCAACAAGAATT+TGG - chr1_3:74844605-74844624 Msa0140620:three_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG + chr1_3:74844651-74844670 Msa0140610:intron 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG - chr1_3:74845505-74845524 Msa0140620:three_prime_UTR 30.0%
CTAGGCCTATTAAAAGATAA+AGG + chr1_3:74845971-74845990 Msa0140610:intron 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG - chr1_3:74846174-74846193 Msa0140620:CDS 30.0%
AGAGAGATAATAAACATCCA+TGG - chr1_3:74846266-74846285 Msa0140620:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG + chr1_3:74846286-74846305 Msa0140610:intron 30.0%
TTTGTCAAATGTGATGTAGA+AGG - chr1_3:74846473-74846492 Msa0140620:intron 30.0%
ATTGTGACGATTATCGATGT+CGG - chr1_3:74844696-74844715 Msa0140620:three_prime_UTR 35.0%
AACGCTAATGTTATCACTGT+TGG - chr1_3:74844741-74844760 Msa0140620:three_prime_UTR 35.0%
AATGTTATCACTGTTGGTGT+CGG - chr1_3:74844747-74844766 Msa0140620:intron 35.0%
ACAAGCAGCAAATTCAATCA+AGG + chr1_3:74844977-74844996 Msa0140610:intron 35.0%
CATTTCCCTCATCAACATAT+CGG - chr1_3:74845210-74845229 Msa0140620:intron 35.0%
AGGAATGTGAAAGACAAAAG+TGG + chr1_3:74845265-74845284 Msa0140610:intron 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG - chr1_3:74845297-74845316 Msa0140620:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG + chr1_3:74845350-74845369 Msa0140610:intron 35.0%
! AGTTCACTTTCTCAACGTTT+TGG + chr1_3:74845351-74845370 Msa0140610:intron 35.0%
AAAACGTTGAGAAAGTGAAC+TGG - chr1_3:74845350-74845369 Msa0140620:intron 35.0%
AAACGTTGAGAAAGTGAACT+GGG - chr1_3:74845351-74845370 Msa0140620:intron 35.0%
TCTGATTAGAGTTACTCATG+AGG - chr1_3:74845636-74845655 Msa0140620:intron 35.0%
ATCTCAACACACCATATCAT+AGG + chr1_3:74845668-74845687 Msa0140610:intron 35.0%
TGTGTTGAGATTTAGTGTTC+TGG - chr1_3:74845677-74845696 Msa0140620:three_prime_UTR 35.0%
TTGCACCTACAGTTTCATTA+TGG - chr1_3:74845855-74845874 Msa0140620:intron 35.0%
ACTTGCCATAATGAAACTGT+AGG + chr1_3:74845863-74845882 Msa0140610:intron 35.0%
! GACTGACTTAATGGTACTAA+TGG + chr1_3:74846095-74846114 Msa0140610:intron 35.0%
TAGTACCATTAAGTCAGTCT+TGG - chr1_3:74846096-74846115 Msa0140620:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG - chr1_3:74846181-74846200 Msa0140620:CDS 35.0%
TTCATCACATTGGCAATCAT+TGG + chr1_3:74846641-74846660 Msa0140610:intron 35.0%
GTATTCAGGAGATATTCTTG+AGG + chr1_3:74846665-74846684 Msa0140610:intron 35.0%
ATTGTGACGATTATCGATGT+CGG - chr1_3:74844696-74844715 Msa0140620:three_prime_UTR 35.0%
AACGCTAATGTTATCACTGT+TGG - chr1_3:74844741-74844760 Msa0140620:three_prime_UTR 35.0%
AATGTTATCACTGTTGGTGT+CGG - chr1_3:74844747-74844766 Msa0140620:intron 35.0%
ACAAGCAGCAAATTCAATCA+AGG + chr1_3:74844977-74844996 Msa0140610:intron 35.0%
CATTTCCCTCATCAACATAT+CGG - chr1_3:74845210-74845229 Msa0140620:intron 35.0%
AGGAATGTGAAAGACAAAAG+TGG + chr1_3:74845265-74845284 Msa0140610:intron 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG - chr1_3:74845297-74845316 Msa0140620:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG + chr1_3:74845350-74845369 Msa0140610:intron 35.0%
! AGTTCACTTTCTCAACGTTT+TGG + chr1_3:74845351-74845370 Msa0140610:intron 35.0%
AAAACGTTGAGAAAGTGAAC+TGG - chr1_3:74845350-74845369 Msa0140620:intron 35.0%
AAACGTTGAGAAAGTGAACT+GGG - chr1_3:74845351-74845370 Msa0140620:intron 35.0%
TCTGATTAGAGTTACTCATG+AGG - chr1_3:74845636-74845655 Msa0140620:intron 35.0%
ATCTCAACACACCATATCAT+AGG + chr1_3:74845668-74845687 Msa0140610:intron 35.0%
TGTGTTGAGATTTAGTGTTC+TGG - chr1_3:74845677-74845696 Msa0140620:three_prime_UTR 35.0%
TTGCACCTACAGTTTCATTA+TGG - chr1_3:74845855-74845874 Msa0140620:intron 35.0%
ACTTGCCATAATGAAACTGT+AGG + chr1_3:74845863-74845882 Msa0140610:intron 35.0%
! GACTGACTTAATGGTACTAA+TGG + chr1_3:74846095-74846114 Msa0140610:intron 35.0%
TAGTACCATTAAGTCAGTCT+TGG - chr1_3:74846096-74846115 Msa0140620:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG - chr1_3:74846181-74846200 Msa0140620:CDS 35.0%
TTCATCACATTGGCAATCAT+TGG + chr1_3:74846641-74846660 Msa0140610:intron 35.0%
GTATTCAGGAGATATTCTTG+AGG + chr1_3:74846665-74846684 Msa0140610:intron 35.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG - chr1_3:74844528-74844547 Msa0140620:three_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG + chr1_3:74844616-74844635 Msa0140610:intron 40.0%
CAAGAATTTGGCATCCAACA+TGG - chr1_3:74844617-74844636 Msa0140620:three_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG + chr1_3:74844634-74844653 Msa0140610:intron 40.0%
TATTGCTCTGGTTGTACTCT+CGG - chr1_3:74844779-74844798 Msa0140620:intron 40.0%
AGAGATGAACTTGGATCCAA+TGG + chr1_3:74844828-74844847 Msa0140610:intron 40.0%
CATGCAACAAGAGATGAACT+TGG + chr1_3:74844837-74844856 Msa0140610:intron 40.0%
GCAGTTATCAACATCTTCGT+TGG - chr1_3:74845173-74845192 Msa0140620:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG + chr1_3:74845218-74845237 Msa0140610:intron 40.0%
AGAAAGTGAACTGGGAAACT+TGG - chr1_3:74845359-74845378 Msa0140620:intron 40.0%
GAGTTAGACAGAACGATTCA+TGG - chr1_3:74845388-74845407 Msa0140620:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG - chr1_3:74845598-74845617 Msa0140620:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG - chr1_3:74845639-74845658 Msa0140620:intron 40.0%
TGAGGTGGTTTCCTATGATA+TGG - chr1_3:74845654-74845673 Msa0140620:intron 40.0%
TTAGTGTTCTGGTGCAAATC+AGG - chr1_3:74845688-74845707 Msa0140620:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG - chr1_3:74845768-74845787 Msa0140620:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG - chr1_3:74845769-74845788 Msa0140620:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG - chr1_3:74845872-74845891 Msa0140620:intron 40.0%
! TGTGACCAAGACTGACTTAA+TGG + chr1_3:74846104-74846123 Msa0140610:intron 40.0%
CAGTATGGTTAGGCTATGTT+TGG + chr1_3:74846397-74846416 Msa0140610:intron 40.0%
! AATTTTCCCCATCAGCAGTA+TGG + chr1_3:74846412-74846431 Msa0140610:intron 40.0%
GAAAAGAGACAAACACTAGC+TGG + chr1_3:74846451-74846470 Msa0140610:intron 40.0%
TTCTTGAGGCTTCATCACAT+TGG + chr1_3:74846651-74846670 Msa0140610:intron 40.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG - chr1_3:74844528-74844547 Msa0140620:three_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG + chr1_3:74844616-74844635 Msa0140610:intron 40.0%
CAAGAATTTGGCATCCAACA+TGG - chr1_3:74844617-74844636 Msa0140620:three_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG + chr1_3:74844634-74844653 Msa0140610:intron 40.0%
TATTGCTCTGGTTGTACTCT+CGG - chr1_3:74844779-74844798 Msa0140620:intron 40.0%
AGAGATGAACTTGGATCCAA+TGG + chr1_3:74844828-74844847 Msa0140610:intron 40.0%
CATGCAACAAGAGATGAACT+TGG + chr1_3:74844837-74844856 Msa0140610:intron 40.0%
GCAGTTATCAACATCTTCGT+TGG - chr1_3:74845173-74845192 Msa0140620:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG + chr1_3:74845218-74845237 Msa0140610:intron 40.0%
AGAAAGTGAACTGGGAAACT+TGG - chr1_3:74845359-74845378 Msa0140620:intron 40.0%
GAGTTAGACAGAACGATTCA+TGG - chr1_3:74845388-74845407 Msa0140620:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG - chr1_3:74845598-74845617 Msa0140620:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG - chr1_3:74845639-74845658 Msa0140620:intron 40.0%
TGAGGTGGTTTCCTATGATA+TGG - chr1_3:74845654-74845673 Msa0140620:intron 40.0%
TTAGTGTTCTGGTGCAAATC+AGG - chr1_3:74845688-74845707 Msa0140620:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG - chr1_3:74845768-74845787 Msa0140620:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG - chr1_3:74845769-74845788 Msa0140620:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG - chr1_3:74845872-74845891 Msa0140620:intron 40.0%
! TGTGACCAAGACTGACTTAA+TGG + chr1_3:74846104-74846123 Msa0140610:intron 40.0%
CAGTATGGTTAGGCTATGTT+TGG + chr1_3:74846397-74846416 Msa0140610:intron 40.0%
! AATTTTCCCCATCAGCAGTA+TGG + chr1_3:74846412-74846431 Msa0140610:intron 40.0%
GAAAAGAGACAAACACTAGC+TGG + chr1_3:74846451-74846470 Msa0140610:intron 40.0%
TTCTTGAGGCTTCATCACAT+TGG + chr1_3:74846651-74846670 Msa0140610:intron 40.0%
!! GGTGATTCTTGTGTTTTGGG+AGG - chr1_3:74844527-74844546 Msa0140620:three_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG - chr1_3:74844532-74844551 Msa0140620:three_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG - chr1_3:74844543-74844562 Msa0140620:three_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG - chr1_3:74844544-74844563 Msa0140620:three_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG - chr1_3:74844780-74844799 Msa0140620:intron 45.0%
CTGATGCCGATATGTTGATG+AGG + chr1_3:74845219-74845238 Msa0140610:intron 45.0%
CTCATCAACATATCGGCATC+AGG - chr1_3:74845217-74845236 Msa0140620:intron 45.0%
ATATCGGCATCAGGTCAACA+TGG - chr1_3:74845226-74845245 Msa0140620:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG - chr1_3:74845364-74845383 Msa0140620:intron 45.0%
GAAACTGTAGGTGCAAAACC+AGG + chr1_3:74845851-74845870 Msa0140610:intron 45.0%
TAGCCTAACCATACTGCTGA+TGG - chr1_3:74846401-74846420 Msa0140620:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG - chr1_3:74846402-74846421 Msa0140620:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG - chr1_3:74846612-74846631 Msa0140620:CDS 45.0%
!! GGTGATTCTTGTGTTTTGGG+AGG - chr1_3:74844527-74844546 Msa0140620:three_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG - chr1_3:74844532-74844551 Msa0140620:three_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG - chr1_3:74844543-74844562 Msa0140620:three_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG - chr1_3:74844544-74844563 Msa0140620:three_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG - chr1_3:74844780-74844799 Msa0140620:intron 45.0%
CTGATGCCGATATGTTGATG+AGG + chr1_3:74845219-74845238 Msa0140610:intron 45.0%
CTCATCAACATATCGGCATC+AGG - chr1_3:74845217-74845236 Msa0140620:intron 45.0%
ATATCGGCATCAGGTCAACA+TGG - chr1_3:74845226-74845245 Msa0140620:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG - chr1_3:74845364-74845383 Msa0140620:intron 45.0%
GAAACTGTAGGTGCAAAACC+AGG + chr1_3:74845851-74845870 Msa0140610:intron 45.0%
TAGCCTAACCATACTGCTGA+TGG - chr1_3:74846401-74846420 Msa0140620:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG - chr1_3:74846402-74846421 Msa0140620:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG - chr1_3:74846612-74846631 Msa0140620:CDS 45.0%
CGATGTCGGCGAAGTTAGTT+CGG - chr1_3:74844710-74844729 Msa0140620:three_prime_UTR 50.0%
TCCATTTGCACCTACACTCC+TGG - chr1_3:74845830-74845849 Msa0140620:intron 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG + chr1_3:74845834-74845853 Msa0140610:intron 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG + chr1_3:74845843-74845862 Msa0140610:intron 50.0%
TATCCCTCAGAAGACGTAGC+AGG - chr1_3:74846334-74846353 Msa0140620:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG - chr1_3:74846347-74846366 Msa0140620:intron 50.0%
GCCTAACCATACTGCTGATG+GGG - chr1_3:74846403-74846422 Msa0140620:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG + chr1_3:74846407-74846426 Msa0140610:intron 50.0%
CGATGTCGGCGAAGTTAGTT+CGG - chr1_3:74844710-74844729 Msa0140620:three_prime_UTR 50.0%
TCCATTTGCACCTACACTCC+TGG - chr1_3:74845830-74845849 Msa0140620:intron 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG + chr1_3:74845834-74845853 Msa0140610:intron 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG + chr1_3:74845843-74845862 Msa0140610:intron 50.0%
TATCCCTCAGAAGACGTAGC+AGG - chr1_3:74846334-74846353 Msa0140620:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG - chr1_3:74846347-74846366 Msa0140620:intron 50.0%
GCCTAACCATACTGCTGATG+GGG - chr1_3:74846403-74846422 Msa0140620:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG + chr1_3:74846407-74846426 Msa0140610:intron 50.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG - chr1_3:74844756-74844775 Msa0140620:intron 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG - chr1_3:74844767-74844786 Msa0140620:intron 55.0%
CTCTGGTTGTACTCTCGGGA+AGG - chr1_3:74844784-74844803 Msa0140620:intron 55.0%
GAGCCTGCTACGTCTTCTGA+GGG + chr1_3:74846340-74846359 Msa0140610:intron 55.0%
AGAGCCTGCTACGTCTTCTG+AGG + chr1_3:74846341-74846360 Msa0140610:intron 55.0%
GACGTAGCAGGCTCTTGTGT+AGG - chr1_3:74846346-74846365 Msa0140620:intron 55.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG - chr1_3:74844756-74844775 Msa0140620:intron 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG - chr1_3:74844767-74844786 Msa0140620:intron 55.0%
CTCTGGTTGTACTCTCGGGA+AGG - chr1_3:74844784-74844803 Msa0140620:intron 55.0%
GAGCCTGCTACGTCTTCTGA+GGG + chr1_3:74846340-74846359 Msa0140610:intron 55.0%
AGAGCCTGCTACGTCTTCTG+AGG + chr1_3:74846341-74846360 Msa0140610:intron 55.0%
GACGTAGCAGGCTCTTGTGT+AGG - chr1_3:74846346-74846365 Msa0140620:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 74844526 74846851 74844526 ID=Msa0140620;Name=Msa0140620
chr1_3 mRNA 74844526 74846851 74844526 ID=Msa0140620-mRNA-1;Parent=Msa0140620;Name=Msa0140620-mRNA-1;_AED=0.23;_eAED=0.23;_QI=186|0.8|0.83|1|0.6|0.5|6|428|85
chr1_3 exon 74844526 74844762 74844526 ID=Msa0140620-mRNA-1:exon:26192;Parent=Msa0140620-mRNA-1
chr1_3 exon 74845462 74845587 74845462 ID=Msa0140620-mRNA-1:exon:26191;Parent=Msa0140620-mRNA-1
chr1_3 exon 74845668 74845767 74845668 ID=Msa0140620-mRNA-1:exon:26190;Parent=Msa0140620-mRNA-1
chr1_3 exon 74845992 74846058 74845992 ID=Msa0140620-mRNA-1:exon:26189;Parent=Msa0140620-mRNA-1
chr1_3 exon 74846139 74846200 74846139 ID=Msa0140620-mRNA-1:exon:26188;Parent=Msa0140620-mRNA-1
chr1_3 exon 74846572 74846851 74846572 ID=Msa0140620-mRNA-1:exon:26187;Parent=Msa0140620-mRNA-1
chr1_3 five_prime_UTR 74846666 74846851 74846666 ID=Msa0140620-mRNA-1:five_prime_utr;Parent=Msa0140620-mRNA-1
chr1_3 CDS 74846572 74846665 74846572 ID=Msa0140620-mRNA-1:cds;Parent=Msa0140620-mRNA-1
chr1_3 CDS 74846139 74846200 74846139 ID=Msa0140620-mRNA-1:cds;Parent=Msa0140620-mRNA-1
chr1_3 CDS 74845992 74846058 74845992 ID=Msa0140620-mRNA-1:cds;Parent=Msa0140620-mRNA-1
chr1_3 CDS 74845733 74845767 74845733 ID=Msa0140620-mRNA-1:cds;Parent=Msa0140620-mRNA-1
chr1_3 three_prime_UTR 74845668 74845732 74845668 ID=Msa0140620-mRNA-1:three_prime_utr;Parent=Msa0140620-mRNA-1
chr1_3 three_prime_UTR 74845462 74845587 74845462 ID=Msa0140620-mRNA-1:three_prime_utr;Parent=Msa0140620-mRNA-1
chr1_3 three_prime_UTR 74844526 74844762 74844526 ID=Msa0140620-mRNA-1:three_prime_utr;Parent=Msa0140620-mRNA-1
Gene Sequence

>Msa0140620

ATGTCGGCGAAGTTAGTTCGGAAATATTCAACGCTAATGTTATCACTGTTGGTGTCGGTGTTGCTGGTATTGCTCTGGTTGTACTCTCGGGAAGTTATCAACATCTTCGTTGGCATTTGTGTATCTGCATTTCCCTCATCAACATATCGGCATCAGGTGAACAAGCTTAAAAGAGAACTTGAAACCCAAAACGTTGAGAAAGTGAACTGGGAAACTTGGATAGGGTTCTCTTGGTTGCAGTTTCTGCAGTCTGATTAG
Protein sequence

>Msa0140620

MSAKLVRKYSTLMLSLLVSVLLVLLWLYSREVINIFVGICVSAFPSSTYRHQVNKLKRELETQNVEKVNWETWIGFSWLQFLQSD*