AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTAACCAACAAGAATT+TGG 0.215065 8_3:+60505532 Msa1299350:five_prime_UTR
AGTTCACTTTCTCAACGTTT+TGG 0.264629 8_3:-60506275 None:intergenic
TGAAGGTGATTCTTGTGTTT+TGG 0.303850 8_3:+60505450 None:intergenic
TTGCACCTACAGTTTCATTA+TGG 0.321692 8_3:+60506782 Msa1299350:three_prime_UTR
ACTGTTGGTGTCGGTGTTGC+TGG 0.340769 8_3:+60505683 Msa1299350:CDS
TTAGTGTTCTGGTGCAAATC+AGG 0.390681 8_3:+60506615 Msa1299350:three_prime_UTR
TGTGTTGAGATTTAGTGTTC+TGG 0.393710 8_3:+60506604 Msa1299350:exon
CGATGTCGGCGAAGTTAGTT+CGG 0.393719 8_3:+60505637 Msa1299350:exon
TTCATCACATTGGCAATCAT+TGG 0.396788 8_3:-60507565 None:intergenic
TCCATTTGCACCTACACTCC+TGG 0.409884 8_3:+60506757 Msa1299350:three_prime_UTR
GAAATGAAACATTGTTCTAA+AGG 0.420124 8_3:+60506821 Msa1299350:three_prime_UTR
AGAAAGTGAACTGGGAAACT+TGG 0.423349 8_3:+60506286 Msa1299350:CDS
TATTGCTCTGGTTGTACTCT+CGG 0.424030 8_3:+60505706 Msa1299350:CDS
CTTAAGAAGTGTAATTATGA+AGG 0.426449 8_3:+60505588 Msa1299350:five_prime_UTR
ACCAGGAGTGTAGGTGCAAA+TGG 0.432206 8_3:-60506758 None:intergenic
CGGTGTTGCTGGTATTGCTC+TGG 0.450550 8_3:+60505694 Msa1299350:CDS
TGGTTAATCTCATATACTTA+CGG 0.460145 8_3:-60505520 None:intergenic
TGAGGTGGTTTCCTATGATA+TGG 0.460675 8_3:+60506581 Msa1299350:CDS
TTATGGCAAGTGACAAGATC+AGG 0.462078 8_3:+60506799 Msa1299350:three_prime_UTR
ATCTCAACACACCATATCAT+AGG 0.464654 8_3:-60506592 None:intergenic
CATTTCCCTCATCAACATAT+CGG 0.470848 8_3:+60506137 Msa1299350:CDS
AAAACGTTGAGAAAGTGAAC+TGG 0.473660 8_3:+60506277 Msa1299350:CDS
AACGCTAATGTTATCACTGT+TGG 0.479387 8_3:+60505668 Msa1299350:CDS
CTCATCAACATATCGGCATC+AGG 0.481451 8_3:+60506144 Msa1299350:CDS
GCAGTTATCAACATCTTCGT+TGG 0.491361 8_3:+60506100 Msa1299350:intron
ACTTCTTAAGCATCTTTACA+AGG 0.508307 8_3:-60505575 None:intergenic
GTTGGATGCCAAATTCTTGT+TGG 0.510438 8_3:-60505540 None:intergenic
CAGGCATAAGCAAGTGATGT+AGG 0.512448 8_3:+60507539 Msa1299350:intron
ACAAGGATCTTAAGCCATGT+TGG 0.517216 8_3:-60505558 None:intergenic
ATTGCTCTGGTTGTACTCTC+GGG 0.525362 8_3:+60505707 Msa1299350:CDS
CAAGAATTTGGCATCCAACA+TGG 0.531190 8_3:+60505544 Msa1299350:five_prime_UTR
TTCTTGAGGCTTCATCACAT+TGG 0.566647 8_3:-60507575 None:intergenic
CTCTGGTTGTACTCTCGGGA+AGG 0.577901 8_3:+60505711 Msa1299350:CDS
GATTAGAGTTACTCATGAGG+TGG 0.581584 8_3:+60506566 Msa1299350:CDS
AGGTGCAAAACCAGGAGTGT+AGG 0.604566 8_3:-60506767 None:intergenic
AATGTTATCACTGTTGGTGT+CGG 0.605324 8_3:+60505674 Msa1299350:CDS
AAACGTTGAGAAAGTGAACT+GGG 0.630136 8_3:+60506278 Msa1299350:CDS
GAAACTGTAGGTGCAAAACC+AGG 0.636631 8_3:-60506775 None:intergenic
ATTGTGACGATTATCGATGT+CGG 0.639449 8_3:+60505623 Msa1299350:exon
CTGATGCCGATATGTTGATG+AGG 0.659856 8_3:-60506143 None:intergenic
GTGAACTGGGAAACTTGGAT+AGG 0.670748 8_3:+60506291 Msa1299350:CDS
GTATTCAGGAGATATTCTTG+AGG 0.672009 8_3:-60507589 None:intergenic
TGATGCCGATATGTTGATGA+GGG 0.679239 8_3:-60506142 None:intergenic
ACTTGCCATAATGAAACTGT+AGG 0.704994 8_3:-60506787 None:intergenic
TCTGATTAGAGTTACTCATG+AGG 0.713055 8_3:+60506563 Msa1299350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGATTGATTTTATATTT+GGG + chr8_3:60506063-60506082 Msa1299350:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG + chr8_3:60506662-60506681 Msa1299350:intron 10.0%
!!! TATAGATTGATTTTATATTT+GGG + chr8_3:60506063-60506082 Msa1299350:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG + chr8_3:60506662-60506681 Msa1299350:intron 10.0%
!!! AAGTTTTGATTTGATTTATT+TGG + chr8_3:60505791-60505810 Msa1299350:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG + chr8_3:60506062-60506081 Msa1299350:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG - chr8_3:60506648-60506667 None:intergenic 15.0%
!!! AAGTTTTGATTTGATTTATT+TGG + chr8_3:60505791-60505810 Msa1299350:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG + chr8_3:60506062-60506081 Msa1299350:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG - chr8_3:60506648-60506667 None:intergenic 15.0%
!! AATGAAACAAACAAGAAAAT+AGG - chr8_3:60505951-60505970 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG - chr8_3:60505975-60505994 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG - chr8_3:60506212-60506231 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG + chr8_3:60506213-60506232 Msa1299350:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG + chr8_3:60506890-60506909 Msa1299350:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG - chr8_3:60506916-60506935 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG + chr8_3:60507520-60507539 Msa1299350:intron 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG + chr8_3:60507661-60507680 Msa1299350:three_prime_UTR 20.0%
!!! ATTTTGCATATTAGTTTTGT+AGG + chr8_3:60507759-60507778 Msa1299350:three_prime_UTR 20.0%
!! AATGAAACAAACAAGAAAAT+AGG - chr8_3:60505951-60505970 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG - chr8_3:60505975-60505994 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG - chr8_3:60506212-60506231 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG + chr8_3:60506213-60506232 Msa1299350:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG + chr8_3:60506890-60506909 Msa1299350:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG - chr8_3:60506916-60506935 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG + chr8_3:60507520-60507539 Msa1299350:intron 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG + chr8_3:60507661-60507680 Msa1299350:three_prime_UTR 20.0%
! TGGTTAATCTCATATACTTA+CGG - chr8_3:60505523-60505542 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG + chr8_3:60505588-60505607 Msa1299350:five_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG + chr8_3:60505736-60505755 Msa1299350:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG + chr8_3:60506380-60506399 Msa1299350:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG + chr8_3:60506515-60506534 Msa1299350:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG + chr8_3:60506516-60506535 Msa1299350:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG + chr8_3:60506680-60506699 Msa1299350:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG + chr8_3:60506821-60506840 Msa1299350:three_prime_UTR 25.0%
!! TTATCTGAAATAGAATCCAT+AGG + chr8_3:60507065-60507084 Msa1299350:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG - chr8_3:60507084-60507103 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG + chr8_3:60507085-60507104 Msa1299350:intron 25.0%
!!! TTGATGATTTTGACATTGAA+TGG + chr8_3:60507476-60507495 Msa1299350:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG - chr8_3:60507606-60507625 None:intergenic 25.0%
! TGGTTAATCTCATATACTTA+CGG - chr8_3:60505523-60505542 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG + chr8_3:60505588-60505607 Msa1299350:five_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG + chr8_3:60505736-60505755 Msa1299350:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG + chr8_3:60506380-60506399 Msa1299350:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG + chr8_3:60506515-60506534 Msa1299350:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG + chr8_3:60506516-60506535 Msa1299350:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG + chr8_3:60506680-60506699 Msa1299350:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG + chr8_3:60506821-60506840 Msa1299350:three_prime_UTR 25.0%
!! TTATCTGAAATAGAATCCAT+AGG + chr8_3:60507065-60507084 Msa1299350:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG - chr8_3:60507084-60507103 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG + chr8_3:60507085-60507104 Msa1299350:intron 25.0%
!!! TTGATGATTTTGACATTGAA+TGG + chr8_3:60507476-60507495 Msa1299350:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG - chr8_3:60507606-60507625 None:intergenic 25.0%
GAGATTAACCAACAAGAATT+TGG + chr8_3:60505532-60505551 Msa1299350:five_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG - chr8_3:60505578-60505597 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG + chr8_3:60506432-60506451 Msa1299350:intron 30.0%
CTAGGCCTATTAAAAGATAA+AGG - chr8_3:60506898-60506917 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG + chr8_3:60507101-60507120 Msa1299350:intron 30.0%
AGAGAGATAATAAACATCCA+TGG + chr8_3:60507193-60507212 Msa1299350:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG - chr8_3:60507213-60507232 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG + chr8_3:60507400-60507419 Msa1299350:intron 30.0%
GAGATTAACCAACAAGAATT+TGG + chr8_3:60505532-60505551 Msa1299350:five_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG - chr8_3:60505578-60505597 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG + chr8_3:60506432-60506451 Msa1299350:intron 30.0%
CTAGGCCTATTAAAAGATAA+AGG - chr8_3:60506898-60506917 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG + chr8_3:60507101-60507120 Msa1299350:intron 30.0%
AGAGAGATAATAAACATCCA+TGG + chr8_3:60507193-60507212 Msa1299350:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG - chr8_3:60507213-60507232 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG + chr8_3:60507400-60507419 Msa1299350:intron 30.0%
ATTGTGACGATTATCGATGT+CGG + chr8_3:60505623-60505642 Msa1299350:exon 35.0%
AACGCTAATGTTATCACTGT+TGG + chr8_3:60505668-60505687 Msa1299350:CDS 35.0%
AATGTTATCACTGTTGGTGT+CGG + chr8_3:60505674-60505693 Msa1299350:CDS 35.0%
ACAAGCAGCAAATTCAATCA+AGG - chr8_3:60505904-60505923 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG + chr8_3:60506137-60506156 Msa1299350:CDS 35.0%
AGGAATGTGAAAGACAAAAG+TGG - chr8_3:60506192-60506211 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG + chr8_3:60506224-60506243 Msa1299350:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG - chr8_3:60506277-60506296 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG - chr8_3:60506278-60506297 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG + chr8_3:60506277-60506296 Msa1299350:CDS 35.0%
AAACGTTGAGAAAGTGAACT+GGG + chr8_3:60506278-60506297 Msa1299350:CDS 35.0%
TCTGATTAGAGTTACTCATG+AGG + chr8_3:60506563-60506582 Msa1299350:CDS 35.0%
ATCTCAACACACCATATCAT+AGG - chr8_3:60506595-60506614 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG + chr8_3:60506604-60506623 Msa1299350:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG + chr8_3:60506782-60506801 Msa1299350:three_prime_UTR 35.0%
ACTTGCCATAATGAAACTGT+AGG - chr8_3:60506790-60506809 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG - chr8_3:60507022-60507041 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG + chr8_3:60507023-60507042 Msa1299350:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG + chr8_3:60507108-60507127 Msa1299350:intron 35.0%
TTCATCACATTGGCAATCAT+TGG - chr8_3:60507568-60507587 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG - chr8_3:60507592-60507611 None:intergenic 35.0%
ATTGTGACGATTATCGATGT+CGG + chr8_3:60505623-60505642 Msa1299350:exon 35.0%
AACGCTAATGTTATCACTGT+TGG + chr8_3:60505668-60505687 Msa1299350:CDS 35.0%
AATGTTATCACTGTTGGTGT+CGG + chr8_3:60505674-60505693 Msa1299350:CDS 35.0%
ACAAGCAGCAAATTCAATCA+AGG - chr8_3:60505904-60505923 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG + chr8_3:60506137-60506156 Msa1299350:CDS 35.0%
AGGAATGTGAAAGACAAAAG+TGG - chr8_3:60506192-60506211 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG + chr8_3:60506224-60506243 Msa1299350:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG - chr8_3:60506277-60506296 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG - chr8_3:60506278-60506297 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG + chr8_3:60506277-60506296 Msa1299350:CDS 35.0%
AAACGTTGAGAAAGTGAACT+GGG + chr8_3:60506278-60506297 Msa1299350:CDS 35.0%
TCTGATTAGAGTTACTCATG+AGG + chr8_3:60506563-60506582 Msa1299350:CDS 35.0%
ATCTCAACACACCATATCAT+AGG - chr8_3:60506595-60506614 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG + chr8_3:60506604-60506623 Msa1299350:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG + chr8_3:60506782-60506801 Msa1299350:three_prime_UTR 35.0%
ACTTGCCATAATGAAACTGT+AGG - chr8_3:60506790-60506809 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG - chr8_3:60507022-60507041 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG + chr8_3:60507023-60507042 Msa1299350:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG + chr8_3:60507108-60507127 Msa1299350:intron 35.0%
TTCATCACATTGGCAATCAT+TGG - chr8_3:60507568-60507587 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG - chr8_3:60507592-60507611 None:intergenic 35.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG + chr8_3:60505455-60505474 Msa1299350:five_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG - chr8_3:60505543-60505562 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG + chr8_3:60505544-60505563 Msa1299350:five_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG - chr8_3:60505561-60505580 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG + chr8_3:60505706-60505725 Msa1299350:CDS 40.0%
AGAGATGAACTTGGATCCAA+TGG - chr8_3:60505755-60505774 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG - chr8_3:60505764-60505783 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG + chr8_3:60506100-60506119 Msa1299350:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG - chr8_3:60506145-60506164 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG + chr8_3:60506286-60506305 Msa1299350:CDS 40.0%
GAGTTAGACAGAACGATTCA+TGG + chr8_3:60506315-60506334 Msa1299350:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG + chr8_3:60506525-60506544 Msa1299350:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG + chr8_3:60506566-60506585 Msa1299350:CDS 40.0%
TGAGGTGGTTTCCTATGATA+TGG + chr8_3:60506581-60506600 Msa1299350:CDS 40.0%
TTAGTGTTCTGGTGCAAATC+AGG + chr8_3:60506615-60506634 Msa1299350:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG + chr8_3:60506695-60506714 Msa1299350:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG + chr8_3:60506696-60506715 Msa1299350:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG + chr8_3:60506799-60506818 Msa1299350:three_prime_UTR 40.0%
! TGTGACCAAGACTGACTTAA+TGG - chr8_3:60507031-60507050 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG - chr8_3:60507324-60507343 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG - chr8_3:60507339-60507358 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG - chr8_3:60507378-60507397 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG - chr8_3:60507578-60507597 None:intergenic 40.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG + chr8_3:60505455-60505474 Msa1299350:five_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG - chr8_3:60505543-60505562 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG + chr8_3:60505544-60505563 Msa1299350:five_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG - chr8_3:60505561-60505580 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG + chr8_3:60505706-60505725 Msa1299350:CDS 40.0%
AGAGATGAACTTGGATCCAA+TGG - chr8_3:60505755-60505774 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG - chr8_3:60505764-60505783 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG + chr8_3:60506100-60506119 Msa1299350:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG - chr8_3:60506145-60506164 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG + chr8_3:60506286-60506305 Msa1299350:CDS 40.0%
GAGTTAGACAGAACGATTCA+TGG + chr8_3:60506315-60506334 Msa1299350:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG + chr8_3:60506525-60506544 Msa1299350:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG + chr8_3:60506566-60506585 Msa1299350:CDS 40.0%
TGAGGTGGTTTCCTATGATA+TGG + chr8_3:60506581-60506600 Msa1299350:CDS 40.0%
TTAGTGTTCTGGTGCAAATC+AGG + chr8_3:60506615-60506634 Msa1299350:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG + chr8_3:60506695-60506714 Msa1299350:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG + chr8_3:60506696-60506715 Msa1299350:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG + chr8_3:60506799-60506818 Msa1299350:three_prime_UTR 40.0%
! TGTGACCAAGACTGACTTAA+TGG - chr8_3:60507031-60507050 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG - chr8_3:60507324-60507343 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG - chr8_3:60507339-60507358 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG - chr8_3:60507378-60507397 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG - chr8_3:60507578-60507597 None:intergenic 40.0%
!! GGTGATTCTTGTGTTTTGGG+AGG + chr8_3:60505454-60505473 Msa1299350:five_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG + chr8_3:60505459-60505478 Msa1299350:five_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG + chr8_3:60505470-60505489 Msa1299350:five_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG + chr8_3:60505471-60505490 Msa1299350:five_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG + chr8_3:60505707-60505726 Msa1299350:CDS 45.0%
CTGATGCCGATATGTTGATG+AGG - chr8_3:60506146-60506165 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG + chr8_3:60506144-60506163 Msa1299350:CDS 45.0%
ATATCGGCATCAGGTCAACA+TGG + chr8_3:60506153-60506172 Msa1299350:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG + chr8_3:60506291-60506310 Msa1299350:CDS 45.0%
GAAACTGTAGGTGCAAAACC+AGG - chr8_3:60506778-60506797 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG + chr8_3:60507328-60507347 Msa1299350:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG + chr8_3:60507329-60507348 Msa1299350:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG + chr8_3:60507539-60507558 Msa1299350:intron 45.0%
!! GGTGATTCTTGTGTTTTGGG+AGG + chr8_3:60505454-60505473 Msa1299350:five_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG + chr8_3:60505459-60505478 Msa1299350:five_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG + chr8_3:60505470-60505489 Msa1299350:five_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG + chr8_3:60505471-60505490 Msa1299350:five_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG + chr8_3:60505707-60505726 Msa1299350:CDS 45.0%
CTGATGCCGATATGTTGATG+AGG - chr8_3:60506146-60506165 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG + chr8_3:60506144-60506163 Msa1299350:CDS 45.0%
ATATCGGCATCAGGTCAACA+TGG + chr8_3:60506153-60506172 Msa1299350:intron 45.0%
GTGAACTGGGAAACTTGGAT+AGG + chr8_3:60506291-60506310 Msa1299350:CDS 45.0%
GAAACTGTAGGTGCAAAACC+AGG - chr8_3:60506778-60506797 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG + chr8_3:60507328-60507347 Msa1299350:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG + chr8_3:60507329-60507348 Msa1299350:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG + chr8_3:60507539-60507558 Msa1299350:intron 45.0%
CGATGTCGGCGAAGTTAGTT+CGG + chr8_3:60505637-60505656 Msa1299350:exon 50.0%
TCCATTTGCACCTACACTCC+TGG + chr8_3:60506757-60506776 Msa1299350:three_prime_UTR 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG - chr8_3:60506761-60506780 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG - chr8_3:60506770-60506789 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG + chr8_3:60507261-60507280 Msa1299350:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG + chr8_3:60507274-60507293 Msa1299350:intron 50.0%
GCCTAACCATACTGCTGATG+GGG + chr8_3:60507330-60507349 Msa1299350:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG - chr8_3:60507334-60507353 None:intergenic 50.0%
CGATGTCGGCGAAGTTAGTT+CGG + chr8_3:60505637-60505656 Msa1299350:exon 50.0%
TCCATTTGCACCTACACTCC+TGG + chr8_3:60506757-60506776 Msa1299350:three_prime_UTR 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG - chr8_3:60506761-60506780 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG - chr8_3:60506770-60506789 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG + chr8_3:60507261-60507280 Msa1299350:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG + chr8_3:60507274-60507293 Msa1299350:intron 50.0%
GCCTAACCATACTGCTGATG+GGG + chr8_3:60507330-60507349 Msa1299350:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG - chr8_3:60507334-60507353 None:intergenic 50.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG + chr8_3:60505683-60505702 Msa1299350:CDS 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG + chr8_3:60505694-60505713 Msa1299350:CDS 55.0%
CTCTGGTTGTACTCTCGGGA+AGG + chr8_3:60505711-60505730 Msa1299350:CDS 55.0%
GAGCCTGCTACGTCTTCTGA+GGG - chr8_3:60507267-60507286 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG - chr8_3:60507268-60507287 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG + chr8_3:60507273-60507292 Msa1299350:intron 55.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG + chr8_3:60505683-60505702 Msa1299350:CDS 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG + chr8_3:60505694-60505713 Msa1299350:CDS 55.0%
CTCTGGTTGTACTCTCGGGA+AGG + chr8_3:60505711-60505730 Msa1299350:CDS 55.0%
GAGCCTGCTACGTCTTCTGA+GGG - chr8_3:60507267-60507286 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG - chr8_3:60507268-60507287 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG + chr8_3:60507273-60507292 Msa1299350:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60505453 60507778 60505453 ID=Msa1299350;Name=Msa1299350
chr8_3 mRNA 60505453 60507778 60505453 ID=Msa1299350-mRNA-1;Parent=Msa1299350;Name=Msa1299350-mRNA-1;_AED=0.23;_eAED=0.23;_QI=186|0.5|0.71|0.85|0.66|0.57|7|388|100
chr8_3 exon 60505453 60505732 60505453 ID=Msa1299350-mRNA-1:exon:17693;Parent=Msa1299350-mRNA-1
chr8_3 exon 60506104 60506165 60506104 ID=Msa1299350-mRNA-1:exon:17694;Parent=Msa1299350-mRNA-1
chr8_3 exon 60506246 60506312 60506246 ID=Msa1299350-mRNA-1:exon:17695;Parent=Msa1299350-mRNA-1
chr8_3 exon 60506402 60506406 60506402 ID=Msa1299350-mRNA-1:exon:17696;Parent=Msa1299350-mRNA-1
chr8_3 exon 60506537 60506636 60506537 ID=Msa1299350-mRNA-1:exon:17697;Parent=Msa1299350-mRNA-1
chr8_3 exon 60506717 60506842 60506717 ID=Msa1299350-mRNA-1:exon:17698;Parent=Msa1299350-mRNA-1
chr8_3 exon 60507542 60507778 60507542 ID=Msa1299350-mRNA-1:exon:17699;Parent=Msa1299350-mRNA-1
chr8_3 five_prime_UTR 60505453 60505638 60505453 ID=Msa1299350-mRNA-1:five_prime_utr;Parent=Msa1299350-mRNA-1
chr8_3 CDS 60505639 60505732 60505639 ID=Msa1299350-mRNA-1:cds;Parent=Msa1299350-mRNA-1
chr8_3 CDS 60506104 60506165 60506104 ID=Msa1299350-mRNA-1:cds;Parent=Msa1299350-mRNA-1
chr8_3 CDS 60506246 60506312 60506246 ID=Msa1299350-mRNA-1:cds;Parent=Msa1299350-mRNA-1
chr8_3 CDS 60506402 60506406 60506402 ID=Msa1299350-mRNA-1:cds;Parent=Msa1299350-mRNA-1
chr8_3 CDS 60506537 60506611 60506537 ID=Msa1299350-mRNA-1:cds;Parent=Msa1299350-mRNA-1
chr8_3 three_prime_UTR 60506612 60506636 60506612 ID=Msa1299350-mRNA-1:three_prime_utr;Parent=Msa1299350-mRNA-1
chr8_3 three_prime_UTR 60506717 60506842 60506717 ID=Msa1299350-mRNA-1:three_prime_utr;Parent=Msa1299350-mRNA-1
chr8_3 three_prime_UTR 60507542 60507778 60507542 ID=Msa1299350-mRNA-1:three_prime_utr;Parent=Msa1299350-mRNA-1
Gene Sequence

>Msa1299350

ATGTCGGCGAAGTTAGTTCGGAAATATTCAACGCTAATGTTATCACTGTTGGTGTCGGTGTTGCTGGTATTGCTCTGGTTGTACTCTCGGGAAGTTATCAACATCTTCGTTGGCATTTGTGTATCTGCATTTCCCTCATCAACATATCGGCATCAGGTGAACAAGCTTAAAAGAGAACTTGAAACCCAAAACGTTGAGAAAGTGAACTGGGAAACTTGGATAGGTTGTGGTTCTCTTGGTTGCAGTTTCTGCAGTCTGATTAGAGTTACTCATGAGGTGGTTTCCTATGATATGGTGTGTTGA
Protein sequence

>Msa1299350

MSAKLVRKYSTLMLSLLVSVLLVLLWLYSREVINIFVGICVSAFPSSTYRHQVNKLKRELETQNVEKVNWETWIGCGSLGCSFCSLIRVTHEVVSYDMVC*