AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0695980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTAACCAACAAGAATT+TGG 0.215065 4_4:+65703347 Msa0695980:five_prime_UTR
AGTTCACTTTCTCAACGTTT+TGG 0.264629 4_4:-65704090 None:intergenic
TGAAGGTGATTCTTGTGTTT+TGG 0.303850 4_4:+65703265 None:intergenic
TTGCACCTACAGTTTCATTA+TGG 0.321692 4_4:+65704597 Msa0695980:three_prime_UTR
ACTGTTGGTGTCGGTGTTGC+TGG 0.340769 4_4:+65703498 Msa0695980:CDS
TTAGTGTTCTGGTGCAAATC+AGG 0.390681 4_4:+65704430 Msa0695980:three_prime_UTR
TGTGTTGAGATTTAGTGTTC+TGG 0.393710 4_4:+65704419 Msa0695980:exon
CGATGTCGGCGAAGTTAGTT+CGG 0.393719 4_4:+65703452 Msa0695980:exon
TTCATCACATTGGCAATCAT+TGG 0.396788 4_4:-65705380 None:intergenic
TCCATTTGCACCTACACTCC+TGG 0.409884 4_4:+65704572 Msa0695980:three_prime_UTR
GAAATGAAACATTGTTCTAA+AGG 0.420124 4_4:+65704636 Msa0695980:three_prime_UTR
AGAAAGTGAACTGGGAAACT+TGG 0.423349 4_4:+65704101 Msa0695980:CDS
TATTGCTCTGGTTGTACTCT+CGG 0.424030 4_4:+65703521 Msa0695980:CDS
CTTAAGAAGTGTAATTATGA+AGG 0.426449 4_4:+65703403 Msa0695980:five_prime_UTR
ACCAGGAGTGTAGGTGCAAA+TGG 0.432206 4_4:-65704573 None:intergenic
CGGTGTTGCTGGTATTGCTC+TGG 0.450550 4_4:+65703509 Msa0695980:CDS
TGGTTAATCTCATATACTTA+CGG 0.460145 4_4:-65703335 None:intergenic
TGAGGTGGTTTCCTATGATA+TGG 0.460675 4_4:+65704396 Msa0695980:CDS
TTATGGCAAGTGACAAGATC+AGG 0.462078 4_4:+65704614 Msa0695980:three_prime_UTR
ATCTCAACACACCATATCAT+AGG 0.464654 4_4:-65704407 None:intergenic
CATTTCCCTCATCAACATAT+CGG 0.470848 4_4:+65703952 Msa0695980:CDS
AAAACGTTGAGAAAGTGAAC+TGG 0.473660 4_4:+65704092 Msa0695980:CDS
AACGCTAATGTTATCACTGT+TGG 0.479387 4_4:+65703483 Msa0695980:CDS
CTCATCAACATATCGGCATC+AGG 0.481451 4_4:+65703959 Msa0695980:CDS
GCAGTTATCAACATCTTCGT+TGG 0.491361 4_4:+65703915 Msa0695980:intron
ACTTCTTAAGCATCTTTACA+AGG 0.508307 4_4:-65703390 None:intergenic
GTTGGATGCCAAATTCTTGT+TGG 0.510438 4_4:-65703355 None:intergenic
CAGGCATAAGCAAGTGATGT+AGG 0.512448 4_4:+65705354 Msa0695980:intron
ACAAGGATCTTAAGCCATGT+TGG 0.517216 4_4:-65703373 None:intergenic
ATTGCTCTGGTTGTACTCTC+GGG 0.525362 4_4:+65703522 Msa0695980:CDS
CAAGAATTTGGCATCCAACA+TGG 0.531190 4_4:+65703359 Msa0695980:five_prime_UTR
TTCTTGAGGCTTCATCACAT+TGG 0.566647 4_4:-65705390 None:intergenic
CTCTGGTTGTACTCTCGGGA+AGG 0.577901 4_4:+65703526 Msa0695980:CDS
GATTAGAGTTACTCATGAGG+TGG 0.581584 4_4:+65704381 Msa0695980:CDS
AGGTGCAAAACCAGGAGTGT+AGG 0.604566 4_4:-65704582 None:intergenic
AATGTTATCACTGTTGGTGT+CGG 0.605324 4_4:+65703489 Msa0695980:CDS
AAACGTTGAGAAAGTGAACT+GGG 0.630136 4_4:+65704093 Msa0695980:CDS
GAAACTGTAGGTGCAAAACC+AGG 0.636631 4_4:-65704590 None:intergenic
ATTGTGACGATTATCGATGT+CGG 0.639449 4_4:+65703438 Msa0695980:exon
CTGATGCCGATATGTTGATG+AGG 0.659856 4_4:-65703958 None:intergenic
GTGAACTGGGAAACTTGGAT+AGG 0.670748 4_4:+65704106 Msa0695980:CDS
GTATTCAGGAGATATTCTTG+AGG 0.672009 4_4:-65705404 None:intergenic
ATATCGGCATCAGGTCAACA+TGG 0.674647 4_4:+65703968 Msa0695980:CDS
TGATGCCGATATGTTGATGA+GGG 0.679239 4_4:-65703957 None:intergenic
ACTTGCCATAATGAAACTGT+AGG 0.704994 4_4:-65704602 None:intergenic
TCTGATTAGAGTTACTCATG+AGG 0.713055 4_4:+65704378 Msa0695980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGATTGATTTTATATTT+GGG + chr4_4:65703878-65703897 Msa0695980:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG + chr4_4:65704477-65704496 Msa0695980:intron 10.0%
!!! TATAGATTGATTTTATATTT+GGG + chr4_4:65703878-65703897 Msa0695980:intron 10.0%
!!! ATATTTTTTTTTTTTGCATA+TGG + chr4_4:65704477-65704496 Msa0695980:intron 10.0%
!!! AAGTTTTGATTTGATTTATT+TGG + chr4_4:65703606-65703625 Msa0695980:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG + chr4_4:65703877-65703896 Msa0695980:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG - chr4_4:65704463-65704482 None:intergenic 15.0%
!!! AAGTTTTGATTTGATTTATT+TGG + chr4_4:65703606-65703625 Msa0695980:intron 15.0%
!!! CTATAGATTGATTTTATATT+TGG + chr4_4:65703877-65703896 Msa0695980:intron 15.0%
!! AAATATGCATAAAAAATTGT+AGG - chr4_4:65704463-65704482 None:intergenic 15.0%
!! AATGAAACAAACAAGAAAAT+AGG - chr4_4:65703766-65703785 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG - chr4_4:65703790-65703809 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG - chr4_4:65704027-65704046 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG + chr4_4:65704028-65704047 Msa0695980:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG + chr4_4:65704705-65704724 Msa0695980:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG - chr4_4:65704731-65704750 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG + chr4_4:65705335-65705354 Msa0695980:intron 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG + chr4_4:65705476-65705495 Msa0695980:three_prime_UTR 20.0%
!!! ATTTTGCATATTAGTTTTGT+AGG + chr4_4:65705574-65705593 Msa0695980:three_prime_UTR 20.0%
!! AATGAAACAAACAAGAAAAT+AGG - chr4_4:65703766-65703785 None:intergenic 20.0%
!! TTACATATAAACAAAACACA+CGG - chr4_4:65703790-65703809 None:intergenic 20.0%
!! AAGTGAGAATAATAAAGAAA+AGG - chr4_4:65704027-65704046 None:intergenic 20.0%
!!! TTCTTTATTATTCTCACTTT+TGG + chr4_4:65704028-65704047 Msa0695980:intron 20.0%
!!! TAGTTCCTTTATCTTTTAAT+AGG + chr4_4:65704705-65704724 Msa0695980:intron 20.0%
!! AGTTTGCATTAAAAATAACT+AGG - chr4_4:65704731-65704750 None:intergenic 20.0%
!!! AGTTTTTTTTACTATTCTAC+AGG + chr4_4:65705335-65705354 Msa0695980:intron 20.0%
!!! GAATCAATGTTTTTTTTAAG+AGG + chr4_4:65705476-65705495 Msa0695980:three_prime_UTR 20.0%
! TGGTTAATCTCATATACTTA+CGG - chr4_4:65703338-65703357 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG + chr4_4:65703403-65703422 Msa0695980:five_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG + chr4_4:65703551-65703570 Msa0695980:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG + chr4_4:65704195-65704214 Msa0695980:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG + chr4_4:65704330-65704349 Msa0695980:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG + chr4_4:65704331-65704350 Msa0695980:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG + chr4_4:65704495-65704514 Msa0695980:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG + chr4_4:65704636-65704655 Msa0695980:three_prime_UTR 25.0%
!! TTATCTGAAATAGAATCCAT+AGG + chr4_4:65704880-65704899 Msa0695980:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG - chr4_4:65704899-65704918 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG + chr4_4:65704900-65704919 Msa0695980:intron 25.0%
!!! TTGATGATTTTGACATTGAA+TGG + chr4_4:65705291-65705310 Msa0695980:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG - chr4_4:65705421-65705440 None:intergenic 25.0%
! TGGTTAATCTCATATACTTA+CGG - chr4_4:65703338-65703357 None:intergenic 25.0%
!! CTTAAGAAGTGTAATTATGA+AGG + chr4_4:65703403-65703422 Msa0695980:five_prime_UTR 25.0%
! TTGTAATTTAGTCAAACCAT+TGG + chr4_4:65703551-65703570 Msa0695980:intron 25.0%
!! GATTTTCAATTCTGTTTTAC+AGG + chr4_4:65704195-65704214 Msa0695980:intron 25.0%
!! TTACTTGTTTGCTTCATTTT+AGG + chr4_4:65704330-65704349 Msa0695980:intron 25.0%
!! TACTTGTTTGCTTCATTTTA+GGG + chr4_4:65704331-65704350 Msa0695980:intron 25.0%
! TATGGTATACTTATTTGAGT+AGG + chr4_4:65704495-65704514 Msa0695980:intron 25.0%
! GAAATGAAACATTGTTCTAA+AGG + chr4_4:65704636-65704655 Msa0695980:three_prime_UTR 25.0%
!! TTATCTGAAATAGAATCCAT+AGG + chr4_4:65704880-65704899 Msa0695980:intron 25.0%
! ATAGACATTCTTAATTCCTA+TGG - chr4_4:65704899-65704918 None:intergenic 25.0%
! AGGAATTAAGAATGTCTATT+TGG + chr4_4:65704900-65704919 Msa0695980:intron 25.0%
!!! TTGATGATTTTGACATTGAA+TGG + chr4_4:65705291-65705310 Msa0695980:intron 25.0%
!! CAATGACTTTTATTGTATTC+AGG - chr4_4:65705421-65705440 None:intergenic 25.0%
GAGATTAACCAACAAGAATT+TGG + chr4_4:65703347-65703366 Msa0695980:five_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG - chr4_4:65703393-65703412 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG + chr4_4:65704247-65704266 Msa0695980:intron 30.0%
CTAGGCCTATTAAAAGATAA+AGG - chr4_4:65704713-65704732 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG + chr4_4:65704916-65704935 Msa0695980:intron 30.0%
AGAGAGATAATAAACATCCA+TGG + chr4_4:65705008-65705027 Msa0695980:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG - chr4_4:65705028-65705047 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG + chr4_4:65705215-65705234 Msa0695980:intron 30.0%
GAGATTAACCAACAAGAATT+TGG + chr4_4:65703347-65703366 Msa0695980:five_prime_UTR 30.0%
ACTTCTTAAGCATCTTTACA+AGG - chr4_4:65703393-65703412 None:intergenic 30.0%
!! GAGTTTGTTACTGTAAATAG+CGG + chr4_4:65704247-65704266 Msa0695980:intron 30.0%
CTAGGCCTATTAAAAGATAA+AGG - chr4_4:65704713-65704732 None:intergenic 30.0%
!! TATTTGGAAGAAAGTGTTGA+AGG + chr4_4:65704916-65704935 Msa0695980:intron 30.0%
AGAGAGATAATAAACATCCA+TGG + chr4_4:65705008-65705027 Msa0695980:intron 30.0%
! TTAGCTTAAGCTTTTAACCA+TGG - chr4_4:65705028-65705047 None:intergenic 30.0%
TTTGTCAAATGTGATGTAGA+AGG + chr4_4:65705215-65705234 Msa0695980:intron 30.0%
ATTGTGACGATTATCGATGT+CGG + chr4_4:65703438-65703457 Msa0695980:exon 35.0%
AACGCTAATGTTATCACTGT+TGG + chr4_4:65703483-65703502 Msa0695980:CDS 35.0%
AATGTTATCACTGTTGGTGT+CGG + chr4_4:65703489-65703508 Msa0695980:CDS 35.0%
ACAAGCAGCAAATTCAATCA+AGG - chr4_4:65703719-65703738 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG + chr4_4:65703952-65703971 Msa0695980:CDS 35.0%
AGGAATGTGAAAGACAAAAG+TGG - chr4_4:65704007-65704026 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG + chr4_4:65704039-65704058 Msa0695980:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG - chr4_4:65704092-65704111 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG - chr4_4:65704093-65704112 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG + chr4_4:65704092-65704111 Msa0695980:CDS 35.0%
AAACGTTGAGAAAGTGAACT+GGG + chr4_4:65704093-65704112 Msa0695980:CDS 35.0%
TCTGATTAGAGTTACTCATG+AGG + chr4_4:65704378-65704397 Msa0695980:CDS 35.0%
ATCTCAACACACCATATCAT+AGG - chr4_4:65704410-65704429 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG + chr4_4:65704419-65704438 Msa0695980:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG + chr4_4:65704597-65704616 Msa0695980:three_prime_UTR 35.0%
ACTTGCCATAATGAAACTGT+AGG - chr4_4:65704605-65704624 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG - chr4_4:65704837-65704856 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG + chr4_4:65704838-65704857 Msa0695980:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG + chr4_4:65704923-65704942 Msa0695980:intron 35.0%
TTCATCACATTGGCAATCAT+TGG - chr4_4:65705383-65705402 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG - chr4_4:65705407-65705426 None:intergenic 35.0%
ATTGTGACGATTATCGATGT+CGG + chr4_4:65703438-65703457 Msa0695980:exon 35.0%
AACGCTAATGTTATCACTGT+TGG + chr4_4:65703483-65703502 Msa0695980:CDS 35.0%
AATGTTATCACTGTTGGTGT+CGG + chr4_4:65703489-65703508 Msa0695980:CDS 35.0%
ACAAGCAGCAAATTCAATCA+AGG - chr4_4:65703719-65703738 None:intergenic 35.0%
CATTTCCCTCATCAACATAT+CGG + chr4_4:65703952-65703971 Msa0695980:CDS 35.0%
AGGAATGTGAAAGACAAAAG+TGG - chr4_4:65704007-65704026 None:intergenic 35.0%
!! TCTCACTTTTGGTTAAATGC+AGG + chr4_4:65704039-65704058 Msa0695980:intron 35.0%
! GTTCACTTTCTCAACGTTTT+GGG - chr4_4:65704092-65704111 None:intergenic 35.0%
! AGTTCACTTTCTCAACGTTT+TGG - chr4_4:65704093-65704112 None:intergenic 35.0%
AAAACGTTGAGAAAGTGAAC+TGG + chr4_4:65704092-65704111 Msa0695980:CDS 35.0%
AAACGTTGAGAAAGTGAACT+GGG + chr4_4:65704093-65704112 Msa0695980:CDS 35.0%
TCTGATTAGAGTTACTCATG+AGG + chr4_4:65704378-65704397 Msa0695980:CDS 35.0%
ATCTCAACACACCATATCAT+AGG - chr4_4:65704410-65704429 None:intergenic 35.0%
TGTGTTGAGATTTAGTGTTC+TGG + chr4_4:65704419-65704438 Msa0695980:exon 35.0%
TTGCACCTACAGTTTCATTA+TGG + chr4_4:65704597-65704616 Msa0695980:three_prime_UTR 35.0%
ACTTGCCATAATGAAACTGT+AGG - chr4_4:65704605-65704624 None:intergenic 35.0%
! GACTGACTTAATGGTACTAA+TGG - chr4_4:65704837-65704856 None:intergenic 35.0%
TAGTACCATTAAGTCAGTCT+TGG + chr4_4:65704838-65704857 Msa0695980:intron 35.0%
!! AAGAAAGTGTTGAAGGAAAG+TGG + chr4_4:65704923-65704942 Msa0695980:intron 35.0%
TTCATCACATTGGCAATCAT+TGG - chr4_4:65705383-65705402 None:intergenic 35.0%
GTATTCAGGAGATATTCTTG+AGG - chr4_4:65705407-65705426 None:intergenic 35.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG + chr4_4:65703270-65703289 Msa0695980:five_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG - chr4_4:65703358-65703377 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG + chr4_4:65703359-65703378 Msa0695980:five_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG - chr4_4:65703376-65703395 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG + chr4_4:65703521-65703540 Msa0695980:CDS 40.0%
AGAGATGAACTTGGATCCAA+TGG - chr4_4:65703570-65703589 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG - chr4_4:65703579-65703598 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG + chr4_4:65703915-65703934 Msa0695980:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG - chr4_4:65703960-65703979 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG + chr4_4:65704101-65704120 Msa0695980:CDS 40.0%
GAGTTAGACAGAACGATTCA+TGG + chr4_4:65704130-65704149 Msa0695980:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG + chr4_4:65704340-65704359 Msa0695980:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG + chr4_4:65704381-65704400 Msa0695980:CDS 40.0%
TGAGGTGGTTTCCTATGATA+TGG + chr4_4:65704396-65704415 Msa0695980:CDS 40.0%
TTAGTGTTCTGGTGCAAATC+AGG + chr4_4:65704430-65704449 Msa0695980:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG + chr4_4:65704510-65704529 Msa0695980:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG + chr4_4:65704511-65704530 Msa0695980:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG + chr4_4:65704614-65704633 Msa0695980:three_prime_UTR 40.0%
! TGTGACCAAGACTGACTTAA+TGG - chr4_4:65704846-65704865 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG - chr4_4:65705139-65705158 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG - chr4_4:65705154-65705173 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG - chr4_4:65705193-65705212 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG - chr4_4:65705393-65705412 None:intergenic 40.0%
!!! GTGATTCTTGTGTTTTGGGA+GGG + chr4_4:65703270-65703289 Msa0695980:five_prime_UTR 40.0%
GTTGGATGCCAAATTCTTGT+TGG - chr4_4:65703358-65703377 None:intergenic 40.0%
CAAGAATTTGGCATCCAACA+TGG + chr4_4:65703359-65703378 Msa0695980:five_prime_UTR 40.0%
ACAAGGATCTTAAGCCATGT+TGG - chr4_4:65703376-65703395 None:intergenic 40.0%
TATTGCTCTGGTTGTACTCT+CGG + chr4_4:65703521-65703540 Msa0695980:CDS 40.0%
AGAGATGAACTTGGATCCAA+TGG - chr4_4:65703570-65703589 None:intergenic 40.0%
CATGCAACAAGAGATGAACT+TGG - chr4_4:65703579-65703598 None:intergenic 40.0%
GCAGTTATCAACATCTTCGT+TGG + chr4_4:65703915-65703934 Msa0695980:intron 40.0%
! TGATGCCGATATGTTGATGA+GGG - chr4_4:65703960-65703979 None:intergenic 40.0%
AGAAAGTGAACTGGGAAACT+TGG + chr4_4:65704101-65704120 Msa0695980:CDS 40.0%
GAGTTAGACAGAACGATTCA+TGG + chr4_4:65704130-65704149 Msa0695980:intron 40.0%
!!! GCTTCATTTTAGGGTTCTCT+TGG + chr4_4:65704340-65704359 Msa0695980:intron 40.0%
GATTAGAGTTACTCATGAGG+TGG + chr4_4:65704381-65704400 Msa0695980:CDS 40.0%
TGAGGTGGTTTCCTATGATA+TGG + chr4_4:65704396-65704415 Msa0695980:CDS 40.0%
TTAGTGTTCTGGTGCAAATC+AGG + chr4_4:65704430-65704449 Msa0695980:three_prime_UTR 40.0%
TGAGTAGGAATGTTGTGTGT+AGG + chr4_4:65704510-65704529 Msa0695980:intron 40.0%
GAGTAGGAATGTTGTGTGTA+GGG + chr4_4:65704511-65704530 Msa0695980:intron 40.0%
TTATGGCAAGTGACAAGATC+AGG + chr4_4:65704614-65704633 Msa0695980:three_prime_UTR 40.0%
! TGTGACCAAGACTGACTTAA+TGG - chr4_4:65704846-65704865 None:intergenic 40.0%
CAGTATGGTTAGGCTATGTT+TGG - chr4_4:65705139-65705158 None:intergenic 40.0%
! AATTTTCCCCATCAGCAGTA+TGG - chr4_4:65705154-65705173 None:intergenic 40.0%
GAAAAGAGACAAACACTAGC+TGG - chr4_4:65705193-65705212 None:intergenic 40.0%
TTCTTGAGGCTTCATCACAT+TGG - chr4_4:65705393-65705412 None:intergenic 40.0%
!! GGTGATTCTTGTGTTTTGGG+AGG + chr4_4:65703269-65703288 Msa0695980:five_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG + chr4_4:65703274-65703293 Msa0695980:five_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG + chr4_4:65703285-65703304 Msa0695980:five_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG + chr4_4:65703286-65703305 Msa0695980:five_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG + chr4_4:65703522-65703541 Msa0695980:CDS 45.0%
CTGATGCCGATATGTTGATG+AGG - chr4_4:65703961-65703980 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG + chr4_4:65703959-65703978 Msa0695980:CDS 45.0%
ATATCGGCATCAGGTCAACA+TGG + chr4_4:65703968-65703987 Msa0695980:CDS 45.0%
GTGAACTGGGAAACTTGGAT+AGG + chr4_4:65704106-65704125 Msa0695980:CDS 45.0%
GAAACTGTAGGTGCAAAACC+AGG - chr4_4:65704593-65704612 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG + chr4_4:65705143-65705162 Msa0695980:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG + chr4_4:65705144-65705163 Msa0695980:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG + chr4_4:65705354-65705373 Msa0695980:intron 45.0%
!! GGTGATTCTTGTGTTTTGGG+AGG + chr4_4:65703269-65703288 Msa0695980:five_prime_UTR 45.0%
!!! TTCTTGTGTTTTGGGAGGGT+AGG + chr4_4:65703274-65703293 Msa0695980:five_prime_UTR 45.0%
TGGGAGGGTAGGAGTAAAAA+TGG + chr4_4:65703285-65703304 Msa0695980:five_prime_UTR 45.0%
GGGAGGGTAGGAGTAAAAAT+GGG + chr4_4:65703286-65703305 Msa0695980:five_prime_UTR 45.0%
ATTGCTCTGGTTGTACTCTC+GGG + chr4_4:65703522-65703541 Msa0695980:CDS 45.0%
CTGATGCCGATATGTTGATG+AGG - chr4_4:65703961-65703980 None:intergenic 45.0%
CTCATCAACATATCGGCATC+AGG + chr4_4:65703959-65703978 Msa0695980:CDS 45.0%
ATATCGGCATCAGGTCAACA+TGG + chr4_4:65703968-65703987 Msa0695980:CDS 45.0%
GTGAACTGGGAAACTTGGAT+AGG + chr4_4:65704106-65704125 Msa0695980:CDS 45.0%
GAAACTGTAGGTGCAAAACC+AGG - chr4_4:65704593-65704612 None:intergenic 45.0%
TAGCCTAACCATACTGCTGA+TGG + chr4_4:65705143-65705162 Msa0695980:intron 45.0%
AGCCTAACCATACTGCTGAT+GGG + chr4_4:65705144-65705163 Msa0695980:intron 45.0%
CAGGCATAAGCAAGTGATGT+AGG + chr4_4:65705354-65705373 Msa0695980:intron 45.0%
CGATGTCGGCGAAGTTAGTT+CGG + chr4_4:65703452-65703471 Msa0695980:exon 50.0%
TCCATTTGCACCTACACTCC+TGG + chr4_4:65704572-65704591 Msa0695980:three_prime_UTR 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG - chr4_4:65704576-65704595 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG - chr4_4:65704585-65704604 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG + chr4_4:65705076-65705095 Msa0695980:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG + chr4_4:65705089-65705108 Msa0695980:intron 50.0%
GCCTAACCATACTGCTGATG+GGG + chr4_4:65705145-65705164 Msa0695980:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG - chr4_4:65705149-65705168 None:intergenic 50.0%
CGATGTCGGCGAAGTTAGTT+CGG + chr4_4:65703452-65703471 Msa0695980:exon 50.0%
TCCATTTGCACCTACACTCC+TGG + chr4_4:65704572-65704591 Msa0695980:three_prime_UTR 50.0%
! ACCAGGAGTGTAGGTGCAAA+TGG - chr4_4:65704576-65704595 None:intergenic 50.0%
! AGGTGCAAAACCAGGAGTGT+AGG - chr4_4:65704585-65704604 None:intergenic 50.0%
TATCCCTCAGAAGACGTAGC+AGG + chr4_4:65705076-65705095 Msa0695980:intron 50.0%
ACGTAGCAGGCTCTTGTGTA+GGG + chr4_4:65705089-65705108 Msa0695980:intron 50.0%
GCCTAACCATACTGCTGATG+GGG + chr4_4:65705145-65705164 Msa0695980:intron 50.0%
TCCCCATCAGCAGTATGGTT+AGG - chr4_4:65705149-65705168 None:intergenic 50.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG + chr4_4:65703498-65703517 Msa0695980:CDS 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG + chr4_4:65703509-65703528 Msa0695980:CDS 55.0%
CTCTGGTTGTACTCTCGGGA+AGG + chr4_4:65703526-65703545 Msa0695980:CDS 55.0%
GAGCCTGCTACGTCTTCTGA+GGG - chr4_4:65705082-65705101 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG - chr4_4:65705083-65705102 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG + chr4_4:65705088-65705107 Msa0695980:intron 55.0%
!! ACTGTTGGTGTCGGTGTTGC+TGG + chr4_4:65703498-65703517 Msa0695980:CDS 55.0%
!! CGGTGTTGCTGGTATTGCTC+TGG + chr4_4:65703509-65703528 Msa0695980:CDS 55.0%
CTCTGGTTGTACTCTCGGGA+AGG + chr4_4:65703526-65703545 Msa0695980:CDS 55.0%
GAGCCTGCTACGTCTTCTGA+GGG - chr4_4:65705082-65705101 None:intergenic 55.0%
AGAGCCTGCTACGTCTTCTG+AGG - chr4_4:65705083-65705102 None:intergenic 55.0%
GACGTAGCAGGCTCTTGTGT+AGG + chr4_4:65705088-65705107 Msa0695980:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 65703268 65705593 65703268 ID=Msa0695980;Name=Msa0695980
chr4_4 mRNA 65703268 65705593 65703268 ID=Msa0695980-mRNA-1;Parent=Msa0695980;Name=Msa0695980-mRNA-1;_AED=0.23;_eAED=0.23;_QI=186|0.66|0.85|0.85|0.66|0.71|7|388|103
chr4_4 exon 65703268 65703547 65703268 ID=Msa0695980-mRNA-1:exon:19026;Parent=Msa0695980-mRNA-1
chr4_4 exon 65703919 65703989 65703919 ID=Msa0695980-mRNA-1:exon:19027;Parent=Msa0695980-mRNA-1
chr4_4 exon 65704061 65704127 65704061 ID=Msa0695980-mRNA-1:exon:19028;Parent=Msa0695980-mRNA-1
chr4_4 exon 65704217 65704221 65704217 ID=Msa0695980-mRNA-1:exon:19029;Parent=Msa0695980-mRNA-1
chr4_4 exon 65704352 65704451 65704352 ID=Msa0695980-mRNA-1:exon:19030;Parent=Msa0695980-mRNA-1
chr4_4 exon 65704532 65704657 65704532 ID=Msa0695980-mRNA-1:exon:19031;Parent=Msa0695980-mRNA-1
chr4_4 exon 65705357 65705593 65705357 ID=Msa0695980-mRNA-1:exon:19032;Parent=Msa0695980-mRNA-1
chr4_4 five_prime_UTR 65703268 65703453 65703268 ID=Msa0695980-mRNA-1:five_prime_utr;Parent=Msa0695980-mRNA-1
chr4_4 CDS 65703454 65703547 65703454 ID=Msa0695980-mRNA-1:cds;Parent=Msa0695980-mRNA-1
chr4_4 CDS 65703919 65703989 65703919 ID=Msa0695980-mRNA-1:cds;Parent=Msa0695980-mRNA-1
chr4_4 CDS 65704061 65704127 65704061 ID=Msa0695980-mRNA-1:cds;Parent=Msa0695980-mRNA-1
chr4_4 CDS 65704217 65704221 65704217 ID=Msa0695980-mRNA-1:cds;Parent=Msa0695980-mRNA-1
chr4_4 CDS 65704352 65704426 65704352 ID=Msa0695980-mRNA-1:cds;Parent=Msa0695980-mRNA-1
chr4_4 three_prime_UTR 65704427 65704451 65704427 ID=Msa0695980-mRNA-1:three_prime_utr;Parent=Msa0695980-mRNA-1
chr4_4 three_prime_UTR 65704532 65704657 65704532 ID=Msa0695980-mRNA-1:three_prime_utr;Parent=Msa0695980-mRNA-1
chr4_4 three_prime_UTR 65705357 65705593 65705357 ID=Msa0695980-mRNA-1:three_prime_utr;Parent=Msa0695980-mRNA-1
Gene Sequence

>Msa0695980

ATGTCGGCGAAGTTAGTTCGGAAATATTCAACGCTAATGTTATCACTGTTGGTGTCGGTGTTGCTGGTATTGCTCTGGTTGTACTCTCGGGAAGTTATCAACATCTTCGTTGGCATTTGTGTATCTGCATTTCCCTCATCAACATATCGGCATCAGGTCAACATGGTGAACAAGCTTAAAAGAGAACTTGAAACCCAAAACGTTGAGAAAGTGAACTGGGAAACTTGGATAGGTTGTGGTTCTCTTGGTTGCAGTTTCTGCAGTCTGATTAGAGTTACTCATGAGGTGGTTTCCTATGATATGGTGTGTTGA
Protein sequence

>Msa0695980

MSAKLVRKYSTLMLSLLVSVLLVLLWLYSREVINIFVGICVSAFPSSTYRHQVNMVNKLKRELETQNVEKVNWETWIGCGSLGCSFCSLIRVTHEVVSYDMVC*