AlfalfaGEDB | Msa0635540

M. sativa cultivar zhongmu-4 / Msa0635540

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0635540	KEH41604.1	40.976	205	70	4	4	169	132	324	3.12e-35	136

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0635540	A0A072VHU7	40.976	205	70	4	4	169	132	324	1.49e-35	136

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0512890	Msa0635540	0.810156	1.325667e-50	-8.615850e-47
Msa0635540	Msa0766630	0.809632	1.719336e-50	-8.615850e-47
Msa0635540	Msa0887990	0.800206	1.606745e-48	-8.615850e-47
Msa0635540	Msa1292400	0.810993	8.742658e-51	-8.615850e-47
Msa0635540	Msa1451570	0.826178	3.129044e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0635540	MtrunA17_Chr1g0206481	45.333	75	38	1	2	73	170	244	9.65e-19	81.3
Msa0635540	MtrunA17_Chr3g0096071	42.857	70	37	1	7	73	61	130	2.23e-17	76.6
Msa0635540	MtrunA17_Chr1g0158831	37.143	70	41	1	5	71	36	105	1.60e-14	68.2
Msa0635540	MtrunA17_Chr2g0278111	37.143	70	41	1	5	71	173	242	1.68e-13	67.0
Msa0635540	MtrunA17_Chr5g0411141	37.143	70	41	1	5	71	174	243	1.76e-13	67.0
Msa0635540	MtrunA17_Chr5g0423441	37.143	70	41	1	5	71	173	242	2.62e-13	65.9
Msa0635540	MtrunA17_Chr2g0280031	38.235	68	39	1	5	69	163	230	6.56e-13	65.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 34 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATGGTTGTTTGTGAGC+TGG	0.523351	4_3:-24688824	Msa0635540:CDS
AAATATATTTACCTGCTCAT+TGG	0.401997	4_3:+24690944	None:intergenic
AAGCAAGGCATTGGGAAAGA+TGG	0.506350	4_3:-24688839	Msa0635540:CDS
AAGTGTCTGATTACATAGAG+AGG	0.648651	4_3:-24689967	Msa0635540:CDS
ACTCCAACAAGCAAGGCATT+GGG	0.499017	4_3:-24688847	Msa0635540:CDS
ACTTCTTAAGCTTGCATAGC+TGG	0.373535	4_3:+24690212	None:intergenic
AGCTGGAATATAACAACCAT+AGG	0.592196	4_3:+24690229	None:intergenic
AGGAGTTGATTCAAGTAACA+AGG	0.598003	4_3:-24688899	Msa0635540:CDS
ATAGAATTCATTGTACCATG+TGG	0.644251	4_3:-24690306	Msa0635540:CDS
ATTTGATGCATCCACAATAA+AGG	0.420859	4_3:-24688548	Msa0635540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTATTTGATCCTTGAAGAT+TGG	+	chr4_3:24688646-24688665	None:intergenic	30.0%
CTTCAAGGATCAAATAGAAA+AGG	-	chr4_3:24688648-24688667	Msa0635540:intron	30.0%
TTCAGATGTTAATGATCATG+TGG	-	chr4_3:24688954-24688973	Msa0635540:intron	30.0%
ATCAAAGAAACTATGAAGGA+TGG	-	chr4_3:24688991-24689010	Msa0635540:intron	30.0%
ATAGAATTCATTGTACCATG+TGG	-	chr4_3:24689148-24689167	Msa0635540:intron	30.0%
TAGAATTCATTGTACCATGT+GGG	-	chr4_3:24689149-24689168	Msa0635540:intron	30.0%
ATGATTTACTTCAAACTCAG+AGG	-	chr4_3:24689681-24689700	Msa0635540:intron	30.0%
TCAAAGTCATTTGACGTTTA+AGG	-	chr4_3:24689862-24689881	Msa0635540:intron	30.0%
CTCTTATAGGAAAATGTTGA+AGG	-	chr4_3:24690535-24690554	Msa0635540:intron	30.0%
ATTTGATGCATCCACAATAA+AGG	-	chr4_3:24690906-24690925	Msa0635540:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	24688513	24690963	24688513	ID=Msa0635540;Name=Msa0635540
chr4_3	mRNA	24688513	24690963	24688513	ID=Msa0635540-mRNA-1;Parent=Msa0635540;Name=Msa0635540-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|0\|0\|0.83\|0.8\|0.83\|6\|0\|177
chr4_3	exon	24690956	24690963	24690956	ID=Msa0635540-mRNA-1:exon:6255;Parent=Msa0635540-mRNA-1
chr4_3	exon	24690206	24690333	24690206	ID=Msa0635540-mRNA-1:exon:6254;Parent=Msa0635540-mRNA-1
chr4_3	exon	24689946	24690043	24689946	ID=Msa0635540-mRNA-1:exon:6253;Parent=Msa0635540-mRNA-1
chr4_3	exon	24689035	24689103	24689035	ID=Msa0635540-mRNA-1:exon:6252;Parent=Msa0635540-mRNA-1
chr4_3	exon	24688807	24688932	24688807	ID=Msa0635540-mRNA-1:exon:6251;Parent=Msa0635540-mRNA-1
chr4_3	exon	24688513	24688617	24688513	ID=Msa0635540-mRNA-1:exon:6250;Parent=Msa0635540-mRNA-1
chr4_3	CDS	24690956	24690963	24690956	ID=Msa0635540-mRNA-1:cds;Parent=Msa0635540-mRNA-1
chr4_3	CDS	24690206	24690333	24690206	ID=Msa0635540-mRNA-1:cds;Parent=Msa0635540-mRNA-1
chr4_3	CDS	24689946	24690043	24689946	ID=Msa0635540-mRNA-1:cds;Parent=Msa0635540-mRNA-1
chr4_3	CDS	24689035	24689103	24689035	ID=Msa0635540-mRNA-1:cds;Parent=Msa0635540-mRNA-1
chr4_3	CDS	24688807	24688932	24688807	ID=Msa0635540-mRNA-1:cds;Parent=Msa0635540-mRNA-1
chr4_3	CDS	24688513	24688617	24688513	ID=Msa0635540-mRNA-1:cds;Parent=Msa0635540-mRNA-1

Gene Sequence

>Msa0635540

ATGAGCAGGGGAAATAGAATTCATTGTACCATGTGGGATGATTATGCTGTGAAAATGCAGCATTGCTTGGATACGCATGACTCATCGCTTCCTATGGTTGTTATATTCCAGCTATGCAAGCTTAAGAAGTATTACGGTATTTCTAATGCATTTTATGGCACGAAAATGATGGTGAATCCTAACATACCTGAAGTGTCTGATTACATAGAGAGGTATGACATATGTGTTTTAAATCTTGCTAATGATGAGGAAGATGTTGCATACGTCGAGACTGTTTGTGAGTTGGCTGATGAAAATGATGTGGAAAATGTTGAAGGAGTTGATTCAAGTAACAAGGACGAAATTACTGTGGTTGATTCTGTTGTGACTCCAACAAGCAAGGCATTGGGAAAGATGGTTGTTTGTGAGCTGGCTGATCAAAATTCAGCGAGAGCTGCTGCTATTGTGGAATCATTGGATGACCTGTCTGTTGATGGATTTGATGCATCCACAATAAAGGAGCCAAAACCAAGCATCGTTTTGGGGAACCGCTGA

Protein sequence

>Msa0635540

MSRGNRIHCTMWDDYAVKMQHCLDTHDSSLPMVVIFQLCKLKKYYGISNAFYGTKMMVNPNIPEVSDYIERYDICVLNLANDEEDVAYVETVCELADENDVENVEGVDSSNKDEITVVDSVVTPTSKALGKMVVCELADQNSARAAAIVESLDDLSVDGFDASTIKEPKPSIVLGNR*