AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0512890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0512890 MtrunA17_Chr8g0391561 53.125 64 20 2 4 63 5 62 3.61e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCCAACGACGGAAATTA+TGG 0.252778 3_4:-62948771 None:intergenic
ACTTCAATTCTGGAAACGTC+TGG 0.392283 3_4:-62948739 None:intergenic
CTTCACACTGCTAGACATCT+AGG 0.444708 3_4:+62948801 Msa0512890:CDS
TGACAATAAGAAGAATGTTT+CGG 0.460187 3_4:+62948654 Msa0512890:CDS
ATGGCCATAATTTCCGTCGT+TGG 0.472194 3_4:+62948767 Msa0512890:CDS
ACAATCTAAGGAATTATGGC+TGG 0.522622 3_4:+62948616 None:intergenic
CTTCAATTCTGGAAACGTCT+GGG 0.550347 3_4:-62948738 None:intergenic
TACTGATCCTCCTCCGACAG+TGG 0.555859 3_4:+62948696 Msa0512890:CDS
TAGGTGACCACTGTCGGAGG+AGG 0.579910 3_4:-62948703 None:intergenic
TTTGCATAGGTGACCACTGT+CGG 0.584337 3_4:-62948709 None:intergenic
GAGGATCAGTAGCAACAACA+AGG 0.606584 3_4:-62948684 None:intergenic
AGATGCGTTCATGCCAACGA+CGG 0.660184 3_4:-62948780 None:intergenic
GCATAGGTGACCACTGTCGG+AGG 0.668853 3_4:-62948706 None:intergenic
AGGATCAGTAGCAACAACAA+GGG 0.691715 3_4:-62948683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGACAATAAGAAGAATGTTT+CGG + chr3_4:62948654-62948673 Msa0512890:CDS 25.0%
! TCCAGAATTGAAGTTTTTGA+TGG + chr3_4:62948748-62948767 Msa0512890:CDS 30.0%
GCCATCAAAAACTTCAATTC+TGG - chr3_4:62948752-62948771 None:intergenic 35.0%
AGGATCAGTAGCAACAACAA+GGG - chr3_4:62948686-62948705 None:intergenic 40.0%
! CTTCAATTCTGGAAACGTCT+GGG - chr3_4:62948741-62948760 None:intergenic 40.0%
! ACTTCAATTCTGGAAACGTC+TGG - chr3_4:62948742-62948761 None:intergenic 40.0%
GAGGATCAGTAGCAACAACA+AGG - chr3_4:62948687-62948706 None:intergenic 45.0%
TTTGCATAGGTGACCACTGT+CGG - chr3_4:62948712-62948731 None:intergenic 45.0%
! GTCTGGGAACTGTTTTGCAT+AGG - chr3_4:62948725-62948744 None:intergenic 45.0%
ATGGCCATAATTTCCGTCGT+TGG + chr3_4:62948767-62948786 Msa0512890:CDS 45.0%
CATGCCAACGACGGAAATTA+TGG - chr3_4:62948774-62948793 None:intergenic 45.0%
AGATGCGTTCATGCCAACGA+CGG - chr3_4:62948783-62948802 None:intergenic 50.0%
TACTGATCCTCCTCCGACAG+TGG + chr3_4:62948696-62948715 Msa0512890:CDS 55.0%
TAGGTGACCACTGTCGGAGG+AGG - chr3_4:62948706-62948725 None:intergenic 60.0%
GCATAGGTGACCACTGTCGG+AGG - chr3_4:62948709-62948728 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 62948631 62948822 62948631 ID=Msa0512890;Name=Msa0512890
chr3_4 mRNA 62948631 62948822 62948631 ID=Msa0512890-mRNA-1;Parent=Msa0512890;Name=Msa0512890-mRNA-1;_AED=0.47;_eAED=0.52;_QI=0|-1|0|1|-1|1|1|0|63
chr3_4 exon 62948631 62948822 62948631 ID=Msa0512890-mRNA-1:exon:15665;Parent=Msa0512890-mRNA-1
chr3_4 CDS 62948631 62948822 62948631 ID=Msa0512890-mRNA-1:cds;Parent=Msa0512890-mRNA-1
Gene Sequence

>Msa0512890

ATGGCTGGAGATGATTCTAACAATGACAATAAGAAGAATGTTTCGGACTCTACCCTTGTTGTTGCTACTGATCCTCCTCCGACAGTGGTCACCTATGCAAAACAGTTCCCAGACGTTTCCAGAATTGAAGTTTTTGATGGCCATAATTTCCGTCGTTGGCATGAACGCATCTTCACACTGCTAGACATCTAG

Protein sequence

>Msa0512890

MAGDDSNNDNKKNVSDSTLVVATDPPPTVVTYAKQFPDVSRIEVFDGHNFRRWHERIFTLLDI*