AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0782330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0782330 MtrunA17_Chr5g0439381 85.905 525 9 3 1 460 1 525 0.0 890
Msa0782330 MtrunA17_Chr5g0399071 45.996 537 198 9 11 460 8 539 6.88e-147 434
Msa0782330 MtrunA17_Chr4g0056431 45.083 539 207 11 9 460 6 542 2.76e-140 417
Msa0782330 MtrunA17_Chr5g0399061 42.533 529 214 9 18 460 17 541 6.70e-139 413
Msa0782330 MtrunA17_Chr4g0063591 46.257 521 198 11 18 460 12 528 1.84e-128 386
Msa0782330 MtrunA17_Chr8g0375311 35.793 542 255 9 4 459 16 550 1.78e-102 319
Msa0782330 MtrunA17_Chr7g0241741 36.127 537 251 9 8 460 2 530 9.88e-100 311
Msa0782330 MtrunA17_Chr8g0375291 35.075 536 259 7 9 460 21 551 1.68e-99 311
Msa0782330 MtrunA17_Chr8g0375341 34.935 541 262 11 3 459 16 550 4.04e-99 310
Msa0782330 MtrunA17_Chr3g0142101 34.766 535 253 10 9 461 16 536 1.61e-98 309
Msa0782330 MtrunA17_Chr8g0375351 34.381 541 265 10 3 459 16 550 8.31e-97 305
Msa0782330 MtrunA17_Chr4g0005901 35.688 538 254 11 7 460 11 540 9.98e-97 304
Msa0782330 MtrunA17_Chr3g0104011 35.448 536 254 10 9 459 3 531 2.33e-96 303
Msa0782330 MtrunA17_Chr8g0375321 34.254 543 268 10 2 460 11 548 5.53e-96 302
Msa0782330 MtrunA17_Chr5g0422881 34.363 518 244 11 28 457 20 529 4.27e-95 300
Msa0782330 MtrunA17_Chr8g0375361 34.783 529 256 9 16 460 27 550 1.54e-94 298
Msa0782330 MtrunA17_Chr8g0375331 33.582 536 266 11 9 460 6 535 2.54e-93 295
Msa0782330 MtrunA17_Chr7g0261811 33.211 545 268 13 1 459 1 535 1.22e-91 291
Msa0782330 MtrunA17_Chr3g0142091 35.203 517 250 9 16 455 14 522 3.81e-91 290
Msa0782330 MtrunA17_Chr1g0166741 34.165 521 259 10 16 459 15 528 2.81e-89 285
Msa0782330 MtrunA17_Chr2g0276751 32.239 518 269 12 16 461 26 533 1.07e-87 281
Msa0782330 MtrunA17_Chr1g0166711 34.340 530 254 12 15 459 12 532 1.10e-86 278
Msa0782330 MtrunA17_Chr7g0261881 33.759 548 261 14 1 459 1 535 1.33e-86 278
Msa0782330 MtrunA17_Chr1g0187241 31.978 541 272 9 10 464 8 538 2.18e-85 275
Msa0782330 MtrunA17_Chr7g0217461 32.472 542 272 9 7 459 3 539 6.60e-85 273
Msa0782330 MtrunA17_Chr1g0166771 34.490 490 237 9 47 459 10 492 2.08e-84 271
Msa0782330 MtrunA17_Chr7g0261771 31.627 547 278 13 1 459 1 539 4.94e-82 266
Msa0782330 MtrunA17_Chr7g0255621 30.496 564 299 11 9 486 3 559 9.91e-82 266
Msa0782330 MtrunA17_Chr1g0166791 32.264 530 266 12 15 459 11 532 1.82e-81 265
Msa0782330 MtrunA17_Chr7g0261851 32.437 558 288 10 1 477 1 550 5.15e-80 261
Msa0782330 MtrunA17_Chr7g0240381 31.664 559 282 16 4 483 6 543 1.27e-79 259
Msa0782330 MtrunA17_Chr3g0114501 29.478 536 282 8 10 459 4 529 6.21e-79 258
Msa0782330 MtrunA17_Chr7g0261791 32.900 538 264 13 9 459 11 538 6.65e-79 258
Msa0782330 MtrunA17_Chr5g0400371 31.171 555 284 10 16 483 100 643 9.07e-79 259
Msa0782330 MtrunA17_Chr4g0008651 31.327 565 283 15 7 483 7 554 3.24e-78 256
Msa0782330 MtrunA17_Chr3g0112671 31.793 541 272 12 9 466 5 531 1.12e-77 254
Msa0782330 MtrunA17_Chr5g0415791 31.853 518 268 9 28 460 29 546 2.20e-77 254
Msa0782330 MtrunA17_Chr4g0054891 30.093 535 287 9 10 460 89 620 2.99e-75 250
Msa0782330 MtrunA17_Chr7g0214731 31.423 541 272 12 13 459 45 580 1.40e-73 245
Msa0782330 MtrunA17_Chr7g0255601 30.458 568 299 13 10 486 4 566 3.19e-72 241
Msa0782330 MtrunA17_Chr7g0255611 29.924 528 278 11 19 460 22 543 1.30e-71 239
Msa0782330 MtrunA17_Chr4g0059971 28.309 544 287 10 7 460 23 553 1.82e-71 238
Msa0782330 MtrunA17_Chr3g0112611 30.556 576 287 16 6 483 11 571 2.06e-71 238
Msa0782330 MtrunA17_Chr8g0362711 30.869 541 280 8 9 460 5 540 3.77e-71 237
Msa0782330 MtrunA17_Chr4g0014871 30.361 527 263 10 16 460 18 522 2.06e-70 234
Msa0782330 MtrunA17_Chr4g0059991 28.374 578 302 12 9 491 12 572 4.49e-70 235
Msa0782330 MtrunA17_Chr7g0214461 29.356 528 285 10 11 459 64 582 4.62e-70 235
Msa0782330 MtrunA17_Chr3g0077151 31.250 528 249 12 16 460 16 512 8.67e-70 233
Msa0782330 MtrunA17_Chr2g0284701 30.410 536 279 11 15 457 19 553 1.07e-69 234
Msa0782330 MtrunA17_Chr1g0213711 30.409 513 274 9 26 460 19 526 1.71e-69 233
Msa0782330 MtrunA17_Chr4g0003761 29.207 517 269 12 32 460 34 541 5.15e-69 232
Msa0782330 MtrunA17_Chr8g0362781 28.670 579 322 10 9 498 11 587 4.23e-68 229
Msa0782330 MtrunA17_Chr4g0014841 30.975 523 262 12 16 460 17 518 2.32e-67 226
Msa0782330 MtrunA17_Chr1g0193391 30.769 494 261 12 28 451 17 499 5.15e-67 227
Msa0782330 MtrunA17_Chr4g0059981 27.239 547 303 11 6 460 7 550 1.26e-66 226
Msa0782330 MtrunA17_Chr4g0014801 30.095 525 266 8 16 460 17 520 5.82e-66 223
Msa0782330 MtrunA17_Chr4g0014781 29.412 527 267 9 16 460 17 520 1.00e-65 222
Msa0782330 MtrunA17_Chr4g0014791 30.426 516 274 10 16 460 17 518 1.67e-65 221
Msa0782330 MtrunA17_Chr7g0218851 29.923 518 276 11 23 459 41 552 2.01e-65 223
Msa0782330 MtrunA17_Chr8g0390331 29.651 516 275 9 28 460 15 525 3.41e-65 221
Msa0782330 MtrunA17_Chr8g0379971 29.091 550 281 12 16 457 8 556 1.25e-64 220
Msa0782330 MtrunA17_Chr7g0261731 36.152 343 175 8 4 306 4 342 1.07e-63 211
Msa0782330 MtrunA17_Chr2g0331471 29.423 520 274 14 28 460 88 601 5.72e-62 215
Msa0782330 MtrunA17_Chr3g0133341 29.094 519 279 12 24 461 10 520 5.85e-61 209
Msa0782330 MtrunA17_Chr4g0011471 30.246 529 280 13 44 488 32 555 9.06e-61 209
Msa0782330 MtrunA17_Chr7g0271221 29.435 496 265 10 28 451 17 499 1.87e-60 209
Msa0782330 MtrunA17_Chr2g0331511 27.500 520 284 13 28 460 21 534 3.03e-60 209
Msa0782330 MtrunA17_Chr2g0331521 27.810 525 284 14 24 460 18 535 6.82e-60 207
Msa0782330 MtrunA17_Chr2g0331481 29.070 516 273 13 32 460 29 538 1.38e-59 207
Msa0782330 MtrunA17_Chr1g0166731 33.023 430 194 12 115 459 9 429 1.94e-59 203
Msa0782330 MtrunA17_Chr4g0060001 28.684 509 278 10 24 452 25 528 7.73e-58 202
Msa0782330 MtrunA17_Chr1g0157601 27.533 523 281 14 26 460 13 525 3.80e-57 199
Msa0782330 MtrunA17_Chr4g0011041 25.044 571 338 12 3 488 12 577 2.86e-56 197
Msa0782330 MtrunA17_Chr7g0240271 25.880 568 330 10 7 487 3 566 1.04e-55 196
Msa0782330 MtrunA17_Chr8g0362801 31.075 428 210 9 118 460 3 430 1.41e-55 193
Msa0782330 MtrunA17_Chr1g0177591 27.593 511 276 12 28 460 42 536 1.06e-54 193
Msa0782330 MtrunA17_Chr7g0261751 42.672 232 127 3 1 228 1 230 2.83e-53 188
Msa0782330 MtrunA17_Chr1g0157611 27.831 521 278 13 28 460 16 526 7.62e-53 188
Msa0782330 MtrunA17_Chr7g0261661 30.465 430 204 12 116 459 1 421 1.09e-52 186
Msa0782330 MtrunA17_Chr3g0142111 42.105 228 127 1 9 236 27 249 1.40e-52 181
Msa0782330 MtrunA17_Chr1g0147631 27.339 545 301 15 28 485 25 561 2.09e-52 187
Msa0782330 MtrunA17_Chr7g0240281 27.256 543 301 12 9 461 4 542 7.57e-51 183
Msa0782330 MtrunA17_Chr1g0157621 28.803 493 258 13 53 460 5 489 8.53e-49 177
Msa0782330 MtrunA17_Chr2g0331461 27.713 516 280 13 32 460 29 538 4.86e-48 176
Msa0782330 MtrunA17_Chr4g0069581 31.365 491 233 15 57 461 15 487 2.74e-46 170
Msa0782330 MtrunA17_Chr5g0415841 27.336 428 224 9 118 460 3 428 3.86e-44 162
Msa0782330 MtrunA17_Chr2g0325951 25.247 507 294 14 29 460 20 516 7.49e-44 162
Msa0782330 MtrunA17_Chr3g0082631 28.818 406 192 9 149 460 26 428 1.30e-43 160
Msa0782330 MtrunA17_Chr1g0177611 29.563 389 192 11 150 460 23 407 4.48e-43 159
Msa0782330 MtrunA17_Chr1g0177631 29.820 389 191 10 150 460 23 407 7.95e-42 155
Msa0782330 MtrunA17_Chr7g0240131 26.974 456 242 11 119 487 4 455 5.60e-40 150
Msa0782330 MtrunA17_Chr7g0235521 26.974 456 242 11 119 487 4 455 1.01e-39 150
Msa0782330 MtrunA17_Chr7g0240211 26.974 456 242 11 119 487 4 455 1.31e-39 150
Msa0782330 MtrunA17_Chr7g0240181 26.974 456 242 11 119 487 4 455 1.37e-39 149
Msa0782330 MtrunA17_Chr5g0415961 39.754 244 131 3 19 262 15 242 3.08e-39 150
Msa0782330 MtrunA17_Chr5g0415961 29.630 243 121 6 260 460 307 541 3.10e-23 103
Msa0782330 MtrunA17_Chr4g0014861 26.566 399 203 9 144 460 7 397 1.40e-37 143
Msa0782330 MtrunA17_Chr3g0142121 36.052 233 108 6 265 461 11 238 1.49e-36 137
Msa0782330 MtrunA17_Chr6g0451841 26.559 497 265 13 47 461 24 502 6.28e-36 140
Msa0782330 MtrunA17_Chr8g0379991 36.757 185 116 1 52 236 4 187 2.97e-34 136
Msa0782330 MtrunA17_Chr8g0379991 28.723 282 142 8 235 457 248 529 3.71e-25 109
Msa0782330 MtrunA17_Chr7g0240151 27.136 398 203 10 119 434 4 396 6.03e-34 133
Msa0782330 MtrunA17_Chr8g0353031 30.769 247 124 7 259 460 275 519 2.28e-31 127
Msa0782330 MtrunA17_Chr8g0353031 37.306 193 99 2 29 221 19 189 1.08e-28 119
Msa0782330 MtrunA17_Chr3g0112631 32.520 246 118 7 264 466 3 243 8.15e-30 118
Msa0782330 MtrunA17_Chr7g0234381 29.730 444 201 16 115 480 3 413 1.63e-28 117
Msa0782330 MtrunA17_Chr7g0234181 27.397 438 213 14 118 480 1 408 3.65e-28 116
Msa0782330 MtrunA17_Chr7g0234151 28.362 409 196 12 115 454 3 383 1.37e-25 108
Msa0782330 MtrunA17_Chr4g0060071 27.966 236 122 4 253 449 25 251 2.08e-25 106
Msa0782330 MtrunA17_Chr4g0014851 44.792 96 53 0 16 111 18 113 4.46e-21 90.1
Msa0782330 MtrunA17_Chr4g0060061 42.553 94 54 0 143 236 8 101 2.10e-19 84.3
Msa0782330 MtrunA17_Chr7g0261671 38.596 114 67 1 1 111 2 115 4.61e-19 83.2
Msa0782330 MtrunA17_Chr4g0060051 48.387 93 48 0 19 111 13 105 3.45e-18 80.9
Msa0782330 MtrunA17_Chr1g0166721 35.606 132 69 3 13 144 11 126 3.60e-18 81.6
Msa0782330 MtrunA17_Chr2g0326211 26.601 203 142 3 26 228 17 212 1.20e-17 85.9
Msa0782330 MtrunA17_Chr2g0325961 30.693 202 133 3 29 230 20 214 5.08e-17 84.0
Msa0782330 MtrunA17_Chr3g0112651 35.075 134 78 3 103 236 9 133 1.23e-16 79.0
Msa0782330 MtrunA17_Chr3g0112661 36.458 96 61 0 16 111 19 114 3.45e-14 69.7
Msa0782330 MtrunA17_Chr7g0261831 30.597 134 72 5 237 356 11 137 1.07e-13 70.5
Msa0782330 MtrunA17_Chr7g0261721 36.559 93 53 2 373 459 77 169 7.73e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0782330 AT3G21670.1 63.740 524 120 4 5 460 6 527 0.0 627
Msa0782330 AT1G12110.1 43.346 526 213 8 18 460 15 538 9.24e-137 408
Msa0782330 AT2G26690.1 46.516 531 198 11 9 459 2 526 1.79e-135 404
Msa0782330 AT2G26690.2 45.473 497 186 10 42 459 3 493 3.47e-120 364
Msa0782330 AT2G26690.3 45.585 487 180 10 52 459 1 481 1.13e-116 354
Msa0782330 AT3G54140.1 35.448 536 256 9 9 460 3 532 1.77e-103 322
Msa0782330 AT3G54140.2 35.448 536 256 9 9 460 3 532 1.77e-103 322
Msa0782330 AT2G02040.1 34.879 539 254 7 9 459 20 549 1.28e-99 312
Msa0782330 AT5G01180.2 34.280 528 256 8 16 459 11 531 1.39e-94 298
Msa0782330 AT5G01180.3 34.280 528 256 8 16 459 11 531 1.39e-94 298
Msa0782330 AT5G01180.1 34.280 528 256 8 16 459 11 531 1.39e-94 298
Msa0782330 AT1G59740.1 33.147 537 272 10 6 461 17 547 3.85e-94 298
Msa0782330 AT1G62200.1 33.714 525 268 8 13 460 35 556 4.30e-93 295
Msa0782330 AT1G62200.2 32.130 554 267 9 13 460 35 585 1.81e-87 281
Msa0782330 AT1G62200.3 32.130 554 267 9 13 460 49 599 2.83e-87 281
Msa0782330 AT1G68570.1 35.509 521 245 11 24 457 17 533 6.96e-86 276
Msa0782330 AT1G68570.2 35.984 503 231 11 42 457 5 503 3.04e-84 271
Msa0782330 AT2G02020.1 33.603 494 277 8 9 457 21 508 3.27e-82 266
Msa0782330 AT5G46050.1 33.396 530 259 11 16 460 13 533 6.43e-82 266
Msa0782330 AT5G46040.1 32.331 532 262 13 16 460 13 533 1.63e-79 259
Msa0782330 AT1G33440.1 32.319 526 269 12 15 461 23 540 4.07e-79 259
Msa0782330 AT2G37900.1 30.077 522 286 8 16 466 24 537 3.41e-78 256
Msa0782330 AT4G21680.1 33.396 533 265 10 15 460 13 542 1.19e-77 255
Msa0782330 AT2G40460.1 31.648 534 257 12 15 459 9 523 2.13e-77 254
Msa0782330 AT1G32450.1 30.565 566 292 11 9 483 14 569 5.17e-77 254
Msa0782330 AT3G53960.1 30.341 557 289 14 16 481 23 571 8.09e-77 253
Msa0782330 AT3G54450.1 29.008 524 261 10 27 460 9 511 5.68e-71 236
Msa0782330 AT5G19640.1 30.080 502 271 9 31 460 60 553 3.60e-69 233
Msa0782330 AT1G72140.1 29.924 528 257 12 18 460 22 521 3.87e-69 231
Msa0782330 AT1G18880.1 29.650 543 282 11 9 460 2 535 1.10e-68 231
Msa0782330 AT5G13400.1 29.630 540 281 11 13 459 46 579 1.29e-68 232
Msa0782330 AT1G27040.1 31.141 517 279 10 20 461 17 531 3.01e-68 229
Msa0782330 AT1G27040.2 31.141 517 279 10 20 461 13 527 3.30e-68 229
Msa0782330 AT3G16180.1 30.095 525 276 11 24 460 16 537 6.42e-68 229
Msa0782330 AT2G37900.2 29.935 461 244 8 77 466 6 458 1.26e-66 223
Msa0782330 AT1G69870.1 29.207 517 278 9 28 459 49 562 1.46e-66 226
Msa0782330 AT1G52190.1 28.411 535 291 11 15 460 7 538 1.85e-66 226
Msa0782330 AT5G62680.1 28.571 518 278 9 33 460 46 561 3.57e-65 223
Msa0782330 AT1G22540.1 31.765 510 273 11 19 460 19 521 5.18e-65 221
Msa0782330 AT1G22570.1 30.948 517 278 12 18 460 18 529 9.06e-63 215
Msa0782330 AT1G27040.3 30.303 495 268 9 42 461 7 499 3.69e-62 213
Msa0782330 AT2G02020.2 30.832 493 271 10 9 457 21 487 2.13e-61 210
Msa0782330 AT3G47960.1 27.992 518 279 9 33 460 64 577 6.25e-61 211
Msa0782330 AT1G72130.1 28.411 535 277 10 1 463 1 501 2.47e-60 207
Msa0782330 AT3G25260.1 29.389 507 264 11 20 461 11 488 9.88e-60 206
Msa0782330 AT1G22550.1 29.710 552 287 15 14 483 12 544 1.07e-59 206
Msa0782330 AT1G27080.1 30.019 513 272 11 28 460 12 517 1.39e-59 206
Msa0782330 AT5G62730.1 28.738 515 268 8 16 449 42 538 4.12e-58 203
Msa0782330 AT3G25280.1 29.225 503 261 11 20 454 11 486 5.45e-58 201
Msa0782330 AT5G46050.2 32.251 431 198 11 116 461 1 422 2.32e-57 198
Msa0782330 AT5G28470.1 26.437 522 298 10 24 467 11 524 1.07e-55 196
Msa0782330 AT5G28470.2 26.336 524 298 10 24 467 11 526 6.40e-55 194
Msa0782330 AT1G72120.1 30.210 523 267 11 18 460 17 521 9.34e-54 190
Msa0782330 AT1G22570.2 29.960 494 267 12 41 460 4 492 4.09e-53 188
Msa0782330 AT5G62730.2 28.226 496 257 8 35 449 2 479 1.53e-52 186
Msa0782330 AT1G72125.1 28.053 524 281 11 18 460 17 525 2.44e-52 187
Msa0782330 AT1G27080.2 29.375 480 252 11 61 460 3 475 1.24e-50 181
Msa0782330 AT1G69860.1 28.963 511 277 13 28 460 16 518 4.86e-50 180
Msa0782330 AT3G45710.1 25.595 504 291 11 30 460 21 513 7.29e-48 174
Msa0782330 AT3G45700.1 24.900 502 292 11 29 460 15 501 1.71e-46 170
Msa0782330 AT3G45720.1 24.609 512 304 11 20 460 9 509 2.35e-46 170
Msa0782330 AT3G45650.1 24.272 515 310 10 16 460 8 512 2.89e-46 170
Msa0782330 AT3G45680.1 24.706 510 299 13 23 460 15 511 3.68e-46 169
Msa0782330 AT5G11570.1 26.580 459 268 10 39 460 22 448 3.18e-44 163
Msa0782330 AT3G45660.1 23.846 520 314 10 12 460 3 511 6.13e-42 158
Msa0782330 AT3G45660.3 23.846 520 314 10 12 460 3 511 6.13e-42 158
Msa0782330 AT3G45660.2 23.846 520 314 10 12 460 24 532 8.56e-42 158
Msa0782330 AT3G45650.2 23.629 529 298 11 28 460 20 538 9.75e-42 158
Msa0782330 AT1G69850.1 40.553 217 124 1 20 236 15 226 3.49e-38 147
Msa0782330 AT1G69850.1 28.049 246 126 4 253 455 305 542 1.44e-24 107
Msa0782330 AT5G14940.1 25.794 504 275 13 47 467 24 511 1.99e-37 145
Msa0782330 AT1G72130.2 26.582 395 202 8 141 463 5 383 5.19e-36 139
Msa0782330 AT1G72130.3 26.582 395 202 8 141 463 5 383 5.19e-36 139
Msa0782330 AT3G01350.1 23.735 514 275 13 47 460 24 520 4.70e-34 135
Msa0782330 AT3G45690.1 24.224 483 286 14 29 460 17 470 3.54e-32 129
Msa0782330 AT2G38100.2 25.271 277 156 8 259 488 218 490 1.26e-15 80.1
Msa0782330 AT2G38100.1 25.271 277 156 8 259 488 234 506 1.75e-15 79.7
Msa0782330 AT2G38100.3 25.271 277 156 8 259 488 240 512 2.06e-15 79.3

Find 101 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACTTGCTTGGCCTTCT+TGG 0.112136 5_2:+65276170 Msa0782330:CDS
TACCATAGTTACCAACTTTA+TGG 0.128785 5_2:+65276142 Msa0782330:CDS
TTATGCTTGCTGCTTGATTC+AGG 0.172720 5_2:+65279017 Msa0782330:CDS
AAATCCAATGTGTCAGGTTT+TGG 0.235332 5_2:+65276806 Msa0782330:CDS
GTAGGAAGAGGTTGGGGATA+TGG 0.242746 5_2:+65276959 Msa0782330:CDS
CAATACAACCACTGAAAATA+AGG 0.259078 5_2:-65276918 None:intergenic
CCAAATCTGTTGAGATTTGC+AGG 0.264980 5_2:+65278941 Msa0782330:CDS
ATCATATTCTTCTCTTCCTT+TGG 0.275627 5_2:-65276854 None:intergenic
GGTCAACATGCATACTCTTT+TGG 0.280969 5_2:+65278157 Msa0782330:CDS
GCACTTTATACAATAGCTTT+AGG 0.298363 5_2:+65276770 Msa0782330:CDS
TCAAGTTGAGGAAGTTAAAA+TGG 0.307630 5_2:+65278113 Msa0782330:CDS
CCTGCAAATCTCAACAGATT+TGG 0.331732 5_2:-65278941 None:intergenic
TCACATATACAGACAAATTC+AGG 0.344673 5_2:+65277169 Msa0782330:CDS
CAAGTATGTCACTAAGTTCA+TGG 0.354511 5_2:-65276084 None:intergenic
ACCATAGTTACCAACTTTAT+GGG 0.356405 5_2:+65276143 Msa0782330:CDS
TAGTGGTCTGGAAGAATATA+AGG 0.371384 5_2:-65275658 None:intergenic
CTAGCAGCAGGACTAATCTT+AGG 0.379230 5_2:+65275937 Msa0782330:CDS
TCCCATAAAGTTGGTAACTA+TGG 0.388879 5_2:-65276144 None:intergenic
GAGTATGCATGTTGACCAAA+TGG 0.390833 5_2:-65278151 None:intergenic
TGTGGCTATGGTTGCAGATA+TGG 0.392408 5_2:-65276237 None:intergenic
TGCTGAAAGAATATGTGTTA+TGG 0.407089 5_2:+65276055 Msa0782330:CDS
TAATCAAACTCCTTCCCATT+TGG 0.408088 5_2:+65278136 Msa0782330:CDS
TATGCTTGCTGCTTGATTCA+GGG 0.411051 5_2:+65279018 Msa0782330:CDS
GCTGAAAGAATATGTGTTAT+GGG 0.417604 5_2:+65276056 Msa0782330:CDS
TTCAGGGAGCTGTCGGCACT+TGG 0.425987 5_2:+65279034 Msa0782330:CDS
AACTTGCTTGGCCTTCTTGG+TGG 0.427150 5_2:+65276173 Msa0782330:CDS
AGACAATGTAGGAAGAGGTT+GGG 0.428903 5_2:+65276952 Msa0782330:CDS
CATGGTGGAGGTGTCATACT+AGG 0.429809 5_2:-65276677 None:intergenic
ATGGGAACTCTCAACTTGCT+TGG 0.432571 5_2:+65276161 Msa0782330:CDS
TAGCTTATTGGTTTCAATTG+TGG 0.435213 5_2:+65278648 Msa0782330:CDS
CATGAACTTAGTGACATACT+TGG 0.438629 5_2:+65276085 Msa0782330:CDS
TAGGTTCTTGTTGCAAGTAA+TGG 0.442403 5_2:+65275835 Msa0782330:intron
CCTTTGAGTTTGTTTCATCT+AGG 0.443818 5_2:-65278050 None:intergenic
CTCATAGATTTCATCCTCTC+AGG 0.449651 5_2:-65278580 None:intergenic
GGAATAAAATCCAATGTGTC+AGG 0.457806 5_2:+65276800 Msa0782330:CDS
GCAACAAAATACTAGTGGTC+TGG 0.461437 5_2:-65275670 None:intergenic
GAATCAAGCAGCAAGCATAA+AGG 0.467644 5_2:-65279014 None:intergenic
TTTCTATGTACACTTTCAAT+GGG 0.473347 5_2:+65278613 Msa0782330:CDS
TTTCTAGCTGATGCAAAACT+TGG 0.477374 5_2:+65276197 Msa0782330:CDS
AAGGTAGGATCAGAAGGGAT+AGG 0.490235 5_2:-65277120 None:intergenic
TGGGGATATGGAATTTCAGC+AGG 0.493923 5_2:+65276971 Msa0782330:CDS
TCTGATCCTACCTTACTCAA+TGG 0.497892 5_2:+65277129 Msa0782330:CDS
TTCAAGATAACCATTGAGTA+AGG 0.498836 5_2:-65277139 None:intergenic
AACTTAGTGACATACTTGGT+TGG 0.500262 5_2:+65276089 Msa0782330:CDS
TAGAGTTCAATTTCAGCGAA+TGG 0.501557 5_2:-65279082 None:intergenic
ACATTCATGAACAGAAAAGT+TGG 0.504425 5_2:+65278222 Msa0782330:CDS
GATGATGAGAAAAGCTGATA+AGG 0.506299 5_2:-65278268 None:intergenic
CTTACTTCACTGCATGGTGG+AGG 0.512876 5_2:-65276689 None:intergenic
TATACAATAGCTTTAGGTGG+TGG 0.514951 5_2:+65276776 Msa0782330:CDS
ACTTGGATTTGTTACCGTGT+TGG 0.515960 5_2:+65279051 Msa0782330:CDS
GTTGAGAGTTCCCATAAAGT+TGG 0.518445 5_2:-65276153 None:intergenic
TTGAGTAAGGTAGGATCAGA+AGG 0.518588 5_2:-65277126 None:intergenic
AAAGCTGATAAGGATCCTGC+TGG 0.518733 5_2:-65278258 None:intergenic
AGGATGAAATCTATGAGTAC+TGG 0.519350 5_2:+65278586 Msa0782330:CDS
GTTTATGTTCAAGACAATGT+AGG 0.523353 5_2:+65276941 Msa0782330:CDS
ACAAAGTAAGCAAGAAAAGA+TGG 0.527615 5_2:+65278671 Msa0782330:CDS
ACAATAGCTTTAGGTGGTGG+AGG 0.528481 5_2:+65276779 Msa0782330:CDS
GTTCAAGACAATGTAGGAAG+AGG 0.528760 5_2:+65276947 Msa0782330:CDS
AAGACAATGTAGGAAGAGGT+TGG 0.532441 5_2:+65276951 Msa0782330:CDS
CAATGGTTATCTTGAAGCTA+AGG 0.534910 5_2:+65277146 Msa0782330:CDS
TGCTTGATTCAGGGAGCTGT+CGG 0.546053 5_2:+65279027 Msa0782330:CDS
TGGATTTGTTACCGTGTTGG+CGG 0.547513 5_2:+65279054 Msa0782330:CDS
GACAATGTAGGAAGAGGTTG+GGG 0.549581 5_2:+65276953 Msa0782330:CDS
TAGGGAAGTAAAGAGGAGAA+TGG 0.551633 5_2:-65278292 None:intergenic
ACTGGAGGATGGCTAGCAGC+AGG 0.553684 5_2:+65275925 Msa0782330:CDS
TCTCATTTAGGGAAGTAAAG+AGG 0.554210 5_2:-65278299 None:intergenic
AGTATGCATGTTGACCAAAT+GGG 0.557444 5_2:-65278150 None:intergenic
TGTCTTCTTACTTCACTGCA+TGG 0.561319 5_2:-65276695 None:intergenic
TTCAACAATGACTCAAGTTG+AGG 0.561755 5_2:+65278101 Msa0782330:CDS
CTATCGTTGAGAAAGAAAGA+AGG 0.562368 5_2:+65278434 Msa0782330:CDS
CTAGGATTGCTGCTTTGTCA+AGG 0.562473 5_2:-65278032 None:intergenic
TGGTAGCAGAGCTAGCTGAA+TGG 0.566779 5_2:-65276124 None:intergenic
AGATAACCATTGAGTAAGGT+AGG 0.570016 5_2:-65277135 None:intergenic
CTTCTTACTTCACTGCATGG+TGG 0.575455 5_2:-65276692 None:intergenic
CATCAGTGCATTCAAGCAAG+TGG 0.588126 5_2:+65276722 Msa0782330:CDS
AGATCCAAAACCTGACACAT+TGG 0.589762 5_2:-65276810 None:intergenic
CAGCTAGAAAACCACCAAGA+AGG 0.599258 5_2:-65276184 None:intergenic
GGGTGTGTGTATGTTAACTT+TGG 0.601232 5_2:+65276643 Msa0782330:intron
ATTTGACACAAATGATCCAA+AGG 0.609233 5_2:+65276838 Msa0782330:CDS
AATTTCAGCAGGGACAATGT+TGG 0.609240 5_2:+65276982 Msa0782330:CDS
CTTTATACAATAGCTTTAGG+TGG 0.613828 5_2:+65276773 Msa0782330:CDS
GTCATTGTTGAAACTAACCA+TGG 0.615360 5_2:-65278090 None:intergenic
ACGGAAACAACGAAAATCCA+TGG 0.615614 5_2:+65278073 Msa0782330:CDS
CCTAGATGAAACAAACTCAA+AGG 0.617218 5_2:+65278050 Msa0782330:CDS
GATGAAACAAACTCAAAGGA+CGG 0.619454 5_2:+65278054 Msa0782330:CDS
GGATCACTAGAGATTCCAGC+AGG 0.623448 5_2:+65278243 Msa0782330:CDS
CTCTAACAGAACATACAACA+TGG 0.625768 5_2:+65275712 Msa0782330:exon
TATCGTTGAGAAAGAAAGAA+GGG 0.638376 5_2:+65278435 Msa0782330:CDS
TTACAGCAACAAAATACTAG+TGG 0.645393 5_2:-65275675 None:intergenic
TGCATGTTGACCAAATGGGA+AGG 0.645769 5_2:-65278146 None:intergenic
CATCTTGTATGCCTACACTA+CGG 0.653414 5_2:+65278972 Msa0782330:CDS
AGCACAAAGTACCGTAGTGT+AGG 0.659207 5_2:-65278983 None:intergenic
TGAGTAAGGTAGGATCAGAA+GGG 0.672680 5_2:-65277125 None:intergenic
TTGAAGAGTAATCTTGACAA+GGG 0.673892 5_2:+65278694 Msa0782330:CDS
TCATAAGGGAAGCACCTGAG+AGG 0.674202 5_2:+65278566 Msa0782330:CDS
GTTGAAGAGTAATCTTGACA+AGG 0.676705 5_2:+65278693 Msa0782330:CDS
GAATGGATCGACCGCCAACA+CGG 0.686480 5_2:-65279065 None:intergenic
ATCTGCAACCATAGCCACAG+TGG 0.695521 5_2:+65276241 Msa0782330:CDS
TGTAGCTTGCTCAATTGTGA+AGG 0.695807 5_2:-65278202 None:intergenic
GGGGATATGGAATTTCAGCA+GGG 0.706056 5_2:+65276972 Msa0782330:CDS
AGGCACAGAAGACAATGCTG+CGG 0.724358 5_2:+65275867 Msa0782330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTTATAAACTACTA+TGG + chr5_2:65276282-65276301 Msa0782330:intron 10.0%
!! AATCCATAAAAAAATTTCTA+AGG + chr5_2:65275968-65275987 Msa0782330:intron 15.0%
!!! TTCTACTTTTTTATAAGTAT+TGG + chr5_2:65276893-65276912 Msa0782330:CDS 15.0%
!! TCATACTTATTACTATTTAT+TGG + chr5_2:65277311-65277330 Msa0782330:intron 15.0%
!! CATACTTATTACTATTTATT+GGG + chr5_2:65277312-65277331 Msa0782330:intron 15.0%
!! ATACTTATTACTATTTATTG+GGG + chr5_2:65277313-65277332 Msa0782330:intron 15.0%
!!! GAACCTTAGAAATTTTTTTA+TGG - chr5_2:65275974-65275993 None:intergenic 20.0%
!!! TTCTAAGGTTCAATTTTATT+TGG + chr5_2:65275983-65276002 Msa0782330:intron 20.0%
!!! ATTATACACAATGATTTTGT+TGG - chr5_2:65276011-65276030 None:intergenic 20.0%
!!! TGTATAATTTTTGTTTGTTC+AGG + chr5_2:65276023-65276042 Msa0782330:intron 20.0%
!! TACTTATTACTATTTATTGG+GGG + chr5_2:65277314-65277333 Msa0782330:intron 20.0%
!!! TGTATTGTATTGTATTCTTT+TGG + chr5_2:65277523-65277542 Msa0782330:intron 20.0%
!! TAAATTATTCCTCGAAATTA+TGG - chr5_2:65277910-65277929 None:intergenic 20.0%
!! GATAATGATAATCTAGAAAA+TGG + chr5_2:65277943-65277962 Msa0782330:intron 20.0%
!!! ACAGTTATTTTTCTCATTTA+GGG - chr5_2:65278313-65278332 None:intergenic 20.0%
!!! TACAGTTATTTTTCTCATTT+AGG - chr5_2:65278314-65278333 None:intergenic 20.0%
!!! ATATTTTGTTAGTAGCTTAT+TGG + chr5_2:65278636-65278655 Msa0782330:CDS 20.0%
! AAGTCATTAACAAAGTTTCA+AGG - chr5_2:65275784-65275803 None:intergenic 25.0%
! TTCTACAACCATAATTGTAT+AGG + chr5_2:65275816-65275835 Msa0782330:intron 25.0%
! AACAAGAACCTATACAATTA+TGG - chr5_2:65275827-65275846 None:intergenic 25.0%
!!! ATTTTTAGAATTGTCTCTGA+GGG + chr5_2:65276353-65276372 Msa0782330:intron 25.0%
! ACGAATTCATGCAAATAAAA+TGG + chr5_2:65276455-65276474 Msa0782330:intron 25.0%
! GAAGTCCTTTAACTATTATT+TGG + chr5_2:65277062-65277081 Msa0782330:intron 25.0%
! TCCTTTAACTATTATTTGGA+GGG + chr5_2:65277066-65277085 Msa0782330:intron 25.0%
! TCTGAAAAGTAAAAACACTT+TGG - chr5_2:65277343-65277362 None:intergenic 25.0%
!! ATTGTATTCTTTTGGTATCA+TGG + chr5_2:65277531-65277550 Msa0782330:intron 25.0%
! CTATGCTAATATATCAACAT+TGG - chr5_2:65277835-65277854 None:intergenic 25.0%
! CGAGGAATAATTTATAGATA+AGG + chr5_2:65277916-65277935 Msa0782330:intron 25.0%
! GAGATTTAATCTATCTTCAT+TGG + chr5_2:65277991-65278010 Msa0782330:intron 25.0%
!! TCATTGGAATTTTGTATGAT+AGG + chr5_2:65278007-65278026 Msa0782330:intron 25.0%
!! AAACTACAATAAGTGCTTTT+TGG + chr5_2:65278476-65278495 Msa0782330:CDS 25.0%
!! ACAGTTAGAGTTTTTCATAA+GGG + chr5_2:65278552-65278571 Msa0782330:CDS 25.0%
!! TTTTCTATGTACACTTTCAA+TGG + chr5_2:65278612-65278631 Msa0782330:CDS 25.0%
! TTTCTATGTACACTTTCAAT+GGG + chr5_2:65278613-65278632 Msa0782330:CDS 25.0%
TTACAGCAACAAAATACTAG+TGG - chr5_2:65275678-65275697 None:intergenic 30.0%
TGCTGAAAGAATATGTGTTA+TGG + chr5_2:65276055-65276074 Msa0782330:CDS 30.0%
GCTGAAAGAATATGTGTTAT+GGG + chr5_2:65276056-65276075 Msa0782330:CDS 30.0%
TACCATAGTTACCAACTTTA+TGG + chr5_2:65276142-65276161 Msa0782330:CDS 30.0%
ACCATAGTTACCAACTTTAT+GGG + chr5_2:65276143-65276162 Msa0782330:CDS 30.0%
! CTCAGAGACAATTCTAAAAA+TGG - chr5_2:65276354-65276373 None:intergenic 30.0%
!!! CATTTTTAGAATTGTCTCTG+AGG + chr5_2:65276352-65276371 Msa0782330:intron 30.0%
!!! TTTTTAGAATTGTCTCTGAG+GGG + chr5_2:65276354-65276373 Msa0782330:intron 30.0%
TTTAAGCTATATCAGCTTAG+TGG - chr5_2:65276421-65276440 None:intergenic 30.0%
TGATATAGCTTAAACGAAAG+TGG + chr5_2:65276427-65276446 Msa0782330:intron 30.0%
GCACTTTATACAATAGCTTT+AGG + chr5_2:65276770-65276789 Msa0782330:CDS 30.0%
!! CTTTATACAATAGCTTTAGG+TGG + chr5_2:65276773-65276792 Msa0782330:CDS 30.0%
ATTTGACACAAATGATCCAA+AGG + chr5_2:65276838-65276857 Msa0782330:CDS 30.0%
ATCATATTCTTCTCTTCCTT+TGG - chr5_2:65276857-65276876 None:intergenic 30.0%
!!! TATTGGATCCTTATTTTCAG+TGG + chr5_2:65276910-65276929 Msa0782330:CDS 30.0%
CAATACAACCACTGAAAATA+AGG - chr5_2:65276921-65276940 None:intergenic 30.0%
!! CTTATTTTCAGTGGTTGTAT+TGG + chr5_2:65276919-65276938 Msa0782330:CDS 30.0%
GTTTATGTTCAAGACAATGT+AGG + chr5_2:65276941-65276960 Msa0782330:CDS 30.0%
GGTTTCTTGAATCTATACAA+TGG - chr5_2:65277037-65277056 None:intergenic 30.0%
TATAGATTCAAGAAACCTCA+AGG + chr5_2:65277040-65277059 Msa0782330:intron 30.0%
GTCCTTTAACTATTATTTGG+AGG + chr5_2:65277065-65277084 Msa0782330:intron 30.0%
ACCCTCCAAATAATAGTTAA+AGG - chr5_2:65277070-65277089 None:intergenic 30.0%
!!! TTATTTGGAGGGTTTTGTTT+TGG + chr5_2:65277077-65277096 Msa0782330:intron 30.0%
TTCAAGATAACCATTGAGTA+AGG - chr5_2:65277142-65277161 None:intergenic 30.0%
TCACATATACAGACAAATTC+AGG + chr5_2:65277169-65277188 Msa0782330:CDS 30.0%
ATATCTTAAATGATGCTGCA+CGG + chr5_2:65277437-65277456 Msa0782330:intron 30.0%
ATTGACACACTTATGTACAA+GGG - chr5_2:65277483-65277502 None:intergenic 30.0%
AATTGACACACTTATGTACA+AGG - chr5_2:65277484-65277503 None:intergenic 30.0%
! GTACATAAGTGTGTCAATTT+TGG + chr5_2:65277485-65277504 Msa0782330:intron 30.0%
TGATTCATTCTTCTCAAGTT+AGG + chr5_2:65277620-65277639 Msa0782330:intron 30.0%
! TGTCTTCGGACTAATATTTT+CGG + chr5_2:65277685-65277704 Msa0782330:intron 30.0%
! AATATTTTCGGATAGATGCA+TGG + chr5_2:65277697-65277716 Msa0782330:intron 30.0%
CGAAAATTACCATAATTTCG+AGG + chr5_2:65277898-65277917 Msa0782330:intron 30.0%
!! TCAAGTTGAGGAAGTTAAAA+TGG + chr5_2:65278113-65278132 Msa0782330:CDS 30.0%
ACATTCATGAACAGAAAAGT+TGG + chr5_2:65278222-65278241 Msa0782330:CDS 30.0%
! TGAGTGATTTTTCTTGCTAA+AGG - chr5_2:65278337-65278356 None:intergenic 30.0%
! CATTTTCTCTATTGTTGCAA+TGG + chr5_2:65278402-65278421 Msa0782330:intron 30.0%
TATCGTTGAGAAAGAAAGAA+GGG + chr5_2:65278435-65278454 Msa0782330:CDS 30.0%
! GACAGTTAGAGTTTTTCATA+AGG + chr5_2:65278551-65278570 Msa0782330:CDS 30.0%
!! TAGCTTATTGGTTTCAATTG+TGG + chr5_2:65278648-65278667 Msa0782330:CDS 30.0%
ACAAAGTAAGCAAGAAAAGA+TGG + chr5_2:65278671-65278690 Msa0782330:CDS 30.0%
TTGAAGAGTAATCTTGACAA+GGG + chr5_2:65278694-65278713 Msa0782330:CDS 30.0%
TAGTGGTCTGGAAGAATATA+AGG - chr5_2:65275661-65275680 None:intergenic 35.0%
CTCTAACAGAACATACAACA+TGG + chr5_2:65275712-65275731 Msa0782330:exon 35.0%
TAGGTTCTTGTTGCAAGTAA+TGG + chr5_2:65275835-65275854 Msa0782330:intron 35.0%
! GCAAGTAATGGAGAGAAAAA+AGG + chr5_2:65275847-65275866 Msa0782330:CDS 35.0%
!!! CAGTTTTTGTTTTGTCAGCT+GGG - chr5_2:65275909-65275928 None:intergenic 35.0%
ACAAAACAAAAACTGGAGGA+TGG + chr5_2:65275914-65275933 Msa0782330:CDS 35.0%
CAAGTATGTCACTAAGTTCA+TGG - chr5_2:65276087-65276106 None:intergenic 35.0%
CATGAACTTAGTGACATACT+TGG + chr5_2:65276085-65276104 Msa0782330:CDS 35.0%
AACTTAGTGACATACTTGGT+TGG + chr5_2:65276089-65276108 Msa0782330:CDS 35.0%
!! TCCCATAAAGTTGGTAACTA+TGG - chr5_2:65276147-65276166 None:intergenic 35.0%
!! TTTCTAGCTGATGCAAAACT+TGG + chr5_2:65276197-65276216 Msa0782330:CDS 35.0%
! AAATTTTACCACTGTGGCTA+TGG - chr5_2:65276252-65276271 None:intergenic 35.0%
TACATGTGTCTCTATCGTTT+CGG + chr5_2:65276548-65276567 Msa0782330:intron 35.0%
GTAGTCAGTAACAATACTTC+AGG - chr5_2:65276593-65276612 None:intergenic 35.0%
ACAATTGTTGTGTTGCAACA+GGG + chr5_2:65276623-65276642 Msa0782330:intron 35.0%
!! TATACAATAGCTTTAGGTGG+TGG + chr5_2:65276776-65276795 Msa0782330:CDS 35.0%
GGAATAAAATCCAATGTGTC+AGG + chr5_2:65276800-65276819 Msa0782330:CDS 35.0%
! AAATCCAATGTGTCAGGTTT+TGG + chr5_2:65276806-65276825 Msa0782330:CDS 35.0%
ATAGTTAAAGGACTTCCTTG+AGG - chr5_2:65277058-65277077 None:intergenic 35.0%
AGATAACCATTGAGTAAGGT+AGG - chr5_2:65277138-65277157 None:intergenic 35.0%
CAATGGTTATCTTGAAGCTA+AGG + chr5_2:65277146-65277165 Msa0782330:CDS 35.0%
! ACGGATATTTTGTCTTGCTT+TGG + chr5_2:65277456-65277475 Msa0782330:intron 35.0%
ATTAGTCCGAAGACAATGAA+GGG - chr5_2:65277680-65277699 None:intergenic 35.0%
TATTAGTCCGAAGACAATGA+AGG - chr5_2:65277681-65277700 None:intergenic 35.0%
AAATTGACACCTCATAGACT+CGG + chr5_2:65277801-65277820 Msa0782330:intron 35.0%
AAATTGAACGAGAATCGCAT+AGG + chr5_2:65277865-65277884 Msa0782330:intron 35.0%
AATTGAACGAGAATCGCATA+GGG + chr5_2:65277866-65277885 Msa0782330:intron 35.0%
GTAAACCAAAAGAAACACTG+TGG + chr5_2:65277965-65277984 Msa0782330:intron 35.0%
! TCTTTCCACAGTGTTTCTTT+TGG - chr5_2:65277973-65277992 None:intergenic 35.0%
! CCTTTGAGTTTGTTTCATCT+AGG - chr5_2:65278053-65278072 None:intergenic 35.0%
CCTAGATGAAACAAACTCAA+AGG + chr5_2:65278050-65278069 Msa0782330:CDS 35.0%
GATGAAACAAACTCAAAGGA+CGG + chr5_2:65278054-65278073 Msa0782330:CDS 35.0%
GTCATTGTTGAAACTAACCA+TGG - chr5_2:65278093-65278112 None:intergenic 35.0%
! TTCAACAATGACTCAAGTTG+AGG + chr5_2:65278101-65278120 Msa0782330:CDS 35.0%
TAATCAAACTCCTTCCCATT+TGG + chr5_2:65278136-65278155 Msa0782330:CDS 35.0%
AGTATGCATGTTGACCAAAT+GGG - chr5_2:65278153-65278172 None:intergenic 35.0%
GATGATGAGAAAAGCTGATA+AGG - chr5_2:65278271-65278290 None:intergenic 35.0%
TCTCATTTAGGGAAGTAAAG+AGG - chr5_2:65278302-65278321 None:intergenic 35.0%
AAAATCACTCACAATCCTCA+AGG + chr5_2:65278346-65278365 Msa0782330:intron 35.0%
CTATCGTTGAGAAAGAAAGA+AGG + chr5_2:65278434-65278453 Msa0782330:CDS 35.0%
!!! GTACCTCAGTTTTTTCTAGT+TGG + chr5_2:65278502-65278521 Msa0782330:CDS 35.0%
AGGATGAAATCTATGAGTAC+TGG + chr5_2:65278586-65278605 Msa0782330:CDS 35.0%
GTTGAAGAGTAATCTTGACA+AGG + chr5_2:65278693-65278712 Msa0782330:CDS 35.0%
! TGTTTGAAACCTGTTGTGTT+TGG + chr5_2:65278888-65278907 Msa0782330:intron 35.0%
GCAACAAAATACTAGTGGTC+TGG - chr5_2:65275673-65275692 None:intergenic 40.0%
!!! CCAGTTTTTGTTTTGTCAGC+TGG - chr5_2:65275910-65275929 None:intergenic 40.0%
CCAGCTGACAAAACAAAAAC+TGG + chr5_2:65275907-65275926 Msa0782330:CDS 40.0%
GCTGACAAAACAAAAACTGG+AGG + chr5_2:65275910-65275929 Msa0782330:CDS 40.0%
GTTGAGAGTTCCCATAAAGT+TGG - chr5_2:65276156-65276175 None:intergenic 40.0%
! GCCACAGTGGTAAAATTTTC+AGG + chr5_2:65276254-65276273 Msa0782330:intron 40.0%
! GCCTGAAAATTTTACCACTG+TGG - chr5_2:65276258-65276277 None:intergenic 40.0%
AATCTCGTAACCTCAAGAGA+CGG - chr5_2:65276389-65276408 None:intergenic 40.0%
GACAATTGTTGTGTTGCAAC+AGG + chr5_2:65276622-65276641 Msa0782330:intron 40.0%
GGGTGTGTGTATGTTAACTT+TGG + chr5_2:65276643-65276662 Msa0782330:intron 40.0%
TGTCTTCTTACTTCACTGCA+TGG - chr5_2:65276698-65276717 None:intergenic 40.0%
AGATCCAAAACCTGACACAT+TGG - chr5_2:65276813-65276832 None:intergenic 40.0%
GTTCAAGACAATGTAGGAAG+AGG + chr5_2:65276947-65276966 Msa0782330:CDS 40.0%
AAGACAATGTAGGAAGAGGT+TGG + chr5_2:65276951-65276970 Msa0782330:CDS 40.0%
AGACAATGTAGGAAGAGGTT+GGG + chr5_2:65276952-65276971 Msa0782330:CDS 40.0%
AATTTCAGCAGGGACAATGT+TGG + chr5_2:65276982-65277001 Msa0782330:CDS 40.0%
!! GCAGTTGGTATTTTGCTTTG+TGG + chr5_2:65277007-65277026 Msa0782330:intron 40.0%
!!! TTGTTTTGGCTTGGAAGAAG+AGG + chr5_2:65277091-65277110 Msa0782330:intron 40.0%
TGAGTAAGGTAGGATCAGAA+GGG - chr5_2:65277128-65277147 None:intergenic 40.0%
TTGAGTAAGGTAGGATCAGA+AGG - chr5_2:65277129-65277148 None:intergenic 40.0%
TCTGATCCTACCTTACTCAA+TGG + chr5_2:65277129-65277148 Msa0782330:CDS 40.0%
CTAGATGCTATAGAAGAGTC+AGG - chr5_2:65277576-65277595 None:intergenic 40.0%
CTTAAGACCATAGCACTTGA+TGG + chr5_2:65277649-65277668 Msa0782330:intron 40.0%
GCATATCCCTTCATTGTCTT+CGG + chr5_2:65277671-65277690 Msa0782330:intron 40.0%
GAGTTGTTCAACGACCATTA+CGG - chr5_2:65277724-65277743 None:intergenic 40.0%
ACGGAAACAACGAAAATCCA+TGG + chr5_2:65278073-65278092 Msa0782330:CDS 40.0%
GAGTATGCATGTTGACCAAA+TGG - chr5_2:65278154-65278173 None:intergenic 40.0%
! GGTCAACATGCATACTCTTT+TGG + chr5_2:65278157-65278176 Msa0782330:CDS 40.0%
!! TGTAGCTTGCTCAATTGTGA+AGG - chr5_2:65278205-65278224 None:intergenic 40.0%
TAGGGAAGTAAAGAGGAGAA+TGG - chr5_2:65278295-65278314 None:intergenic 40.0%
AAGGACTCACAAGTCTTCAA+AGG + chr5_2:65278365-65278384 Msa0782330:intron 40.0%
AGGACTCACAAGTCTTCAAA+GGG + chr5_2:65278366-65278385 Msa0782330:intron 40.0%
AGTCTTCAAAGGGTAGGAAT+TGG + chr5_2:65278376-65278395 Msa0782330:intron 40.0%
GCACCAACTAGAAAAAACTG+AGG - chr5_2:65278508-65278527 None:intergenic 40.0%
!!! CAGTTTTTTCTAGTTGGTGC+TGG + chr5_2:65278508-65278527 Msa0782330:CDS 40.0%
! GGAGAAGCTTTTGCTTATGT+TGG + chr5_2:65278529-65278548 Msa0782330:CDS 40.0%
CTCATAGATTTCATCCTCTC+AGG - chr5_2:65278583-65278602 None:intergenic 40.0%
TTGAAACCTGTTGTGTTTGG+TGG + chr5_2:65278891-65278910 Msa0782330:intron 40.0%
CCTGCAAATCTCAACAGATT+TGG - chr5_2:65278944-65278963 None:intergenic 40.0%
CCAAATCTGTTGAGATTTGC+AGG + chr5_2:65278941-65278960 Msa0782330:CDS 40.0%
CATCTTGTATGCCTACACTA+CGG + chr5_2:65278972-65278991 Msa0782330:CDS 40.0%
GAATCAAGCAGCAAGCATAA+AGG - chr5_2:65279017-65279036 None:intergenic 40.0%
! TTATGCTTGCTGCTTGATTC+AGG + chr5_2:65279017-65279036 Msa0782330:CDS 40.0%
! TATGCTTGCTGCTTGATTCA+GGG + chr5_2:65279018-65279037 Msa0782330:CDS 40.0%
!! ACTTGGATTTGTTACCGTGT+TGG + chr5_2:65279051-65279070 Msa0782330:CDS 40.0%
CTAGCAGCAGGACTAATCTT+AGG + chr5_2:65275937-65275956 Msa0782330:CDS 45.0%
! ATGGGAACTCTCAACTTGCT+TGG + chr5_2:65276161-65276180 Msa0782330:CDS 45.0%
CAGCTAGAAAACCACCAAGA+AGG - chr5_2:65276187-65276206 None:intergenic 45.0%
TGTGGCTATGGTTGCAGATA+TGG - chr5_2:65276240-65276259 None:intergenic 45.0%
GATTTGAACTCCGTCTCTTG+AGG + chr5_2:65276376-65276395 Msa0782330:intron 45.0%
! CTTCAGGTTAAAAGCGTGTC+TGG - chr5_2:65276577-65276596 None:intergenic 45.0%
CTTCTTACTTCACTGCATGG+TGG - chr5_2:65276695-65276714 None:intergenic 45.0%
CATCAGTGCATTCAAGCAAG+TGG + chr5_2:65276722-65276741 Msa0782330:CDS 45.0%
!! ACAATAGCTTTAGGTGGTGG+AGG + chr5_2:65276779-65276798 Msa0782330:CDS 45.0%
GACAATGTAGGAAGAGGTTG+GGG + chr5_2:65276953-65276972 Msa0782330:CDS 45.0%
TGGGGATATGGAATTTCAGC+AGG + chr5_2:65276971-65276990 Msa0782330:CDS 45.0%
GGGGATATGGAATTTCAGCA+GGG + chr5_2:65276972-65276991 Msa0782330:CDS 45.0%
!!! TGGAGGGTTTTGTTTTGGCT+TGG + chr5_2:65277082-65277101 Msa0782330:intron 45.0%
AAGGTAGGATCAGAAGGGAT+AGG - chr5_2:65277123-65277142 None:intergenic 45.0%
GGATATGCCATCAAGTGCTA+TGG - chr5_2:65277659-65277678 None:intergenic 45.0%
GATAGATGCATGGACCGTAA+TGG + chr5_2:65277707-65277726 Msa0782330:intron 45.0%
!! CTAGGATTGCTGCTTTGTCA+AGG - chr5_2:65278035-65278054 None:intergenic 45.0%
TGCATGTTGACCAAATGGGA+AGG - chr5_2:65278149-65278168 None:intergenic 45.0%
AAAGCTGATAAGGATCCTGC+TGG - chr5_2:65278261-65278280 None:intergenic 45.0%
TGAAGACTTGTGAGTCCTTG+AGG - chr5_2:65278364-65278383 None:intergenic 45.0%
CTCACAAGTCTTCAAAGGGT+AGG + chr5_2:65278370-65278389 Msa0782330:intron 45.0%
!! AGCACAAAGTACCGTAGTGT+AGG - chr5_2:65278986-65279005 None:intergenic 45.0%
!! TGGATTTGTTACCGTGTTGG+CGG + chr5_2:65279054-65279073 Msa0782330:CDS 45.0%
AGGCACAGAAGACAATGCTG+CGG + chr5_2:65275867-65275886 Msa0782330:CDS 50.0%
!! AATGCTGCGGTCGATTTTCG+AGG + chr5_2:65275880-65275899 Msa0782330:CDS 50.0%
TGGTAGCAGAGCTAGCTGAA+TGG - chr5_2:65276127-65276146 None:intergenic 50.0%
! CTCAACTTGCTTGGCCTTCT+TGG + chr5_2:65276170-65276189 Msa0782330:CDS 50.0%
! AACTTGCTTGGCCTTCTTGG+TGG + chr5_2:65276173-65276192 Msa0782330:CDS 50.0%
ATCTGCAACCATAGCCACAG+TGG + chr5_2:65276241-65276260 Msa0782330:CDS 50.0%
!! CATGGTGGAGGTGTCATACT+AGG - chr5_2:65276680-65276699 None:intergenic 50.0%
CTTACTTCACTGCATGGTGG+AGG - chr5_2:65276692-65276711 None:intergenic 50.0%
GTAGGAAGAGGTTGGGGATA+TGG + chr5_2:65276959-65276978 Msa0782330:CDS 50.0%
!! GGGACAATGTTGGTAGCAGT+TGG + chr5_2:65276992-65277011 Msa0782330:intron 50.0%
GACTAGTAGCCGAGTCTATG+AGG - chr5_2:65277813-65277832 None:intergenic 50.0%
GGATCACTAGAGATTCCAGC+AGG + chr5_2:65278243-65278262 Msa0782330:CDS 50.0%
!! TCATAAGGGAAGCACCTGAG+AGG + chr5_2:65278566-65278585 Msa0782330:CDS 50.0%
CGCGTAAGAACTCAGATCGA+GGG - chr5_2:65278866-65278885 None:intergenic 50.0%
ACGCGTAAGAACTCAGATCG+AGG - chr5_2:65278867-65278886 None:intergenic 50.0%
GTCAAGCCACCAAACACAAC+AGG - chr5_2:65278900-65278919 None:intergenic 50.0%
TGCTTGATTCAGGGAGCTGT+CGG + chr5_2:65279027-65279046 Msa0782330:CDS 50.0%
GAATGGATCGACCGCCAACA+CGG - chr5_2:65279068-65279087 None:intergenic 55.0%
ACTGGAGGATGGCTAGCAGC+AGG + chr5_2:65275925-65275944 Msa0782330:CDS 60.0%
TTCAGGGAGCTGTCGGCACT+TGG + chr5_2:65279034-65279053 Msa0782330:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 65275622 65279092 65275622 ID=Msa0782330;Name=Msa0782330
chr5_2 mRNA 65275622 65279092 65275622 ID=Msa0782330-mRNA-1;Parent=Msa0782330;Name=Msa0782330-mRNA-1;_AED=0.12;_eAED=0.12;_QI=109|0.57|0.37|0.87|1|1|8|0|514
chr5_2 exon 65275622 65275733 65275622 ID=Msa0782330-mRNA-1:exon:19542;Parent=Msa0782330-mRNA-1
chr5_2 exon 65275838 65275958 65275838 ID=Msa0782330-mRNA-1:exon:19543;Parent=Msa0782330-mRNA-1
chr5_2 exon 65276045 65276262 65276045 ID=Msa0782330-mRNA-1:exon:19544;Parent=Msa0782330-mRNA-1
chr5_2 exon 65276644 65277009 65276644 ID=Msa0782330-mRNA-1:exon:19545;Parent=Msa0782330-mRNA-1
chr5_2 exon 65277108 65277190 65277108 ID=Msa0782330-mRNA-1:exon:19546;Parent=Msa0782330-mRNA-1
chr5_2 exon 65278029 65278330 65278029 ID=Msa0782330-mRNA-1:exon:19547;Parent=Msa0782330-mRNA-1
chr5_2 exon 65278428 65278715 65278428 ID=Msa0782330-mRNA-1:exon:19548;Parent=Msa0782330-mRNA-1
chr5_2 exon 65278929 65279092 65278929 ID=Msa0782330-mRNA-1:exon:19549;Parent=Msa0782330-mRNA-1
chr5_2 five_prime_UTR 65275622 65275730 65275622 ID=Msa0782330-mRNA-1:five_prime_utr;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65275731 65275733 65275731 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65275838 65275958 65275838 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65276045 65276262 65276045 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65276644 65277009 65276644 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65277108 65277190 65277108 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65278029 65278330 65278029 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65278428 65278715 65278428 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
chr5_2 CDS 65278929 65279092 65278929 ID=Msa0782330-mRNA-1:cds;Parent=Msa0782330-mRNA-1
Gene Sequence

>Msa0782330

ATGGTTCTTGTTGCAAGTAATGGAGAGAAAAAAGGCACAGAAGACAATGCTGCGGTCGATTTTCGAGGTCACCCAGCTGACAAAACAAAAACTGGAGGATGGCTAGCAGCAGGACTAATCTTAGGTACTGAACTTGCTGAAAGAATATGTGTTATGGGAATTTCCATGAACTTAGTGACATACTTGGTTGGAGATTTACATCTCCATTCAGCTAGCTCTGCTACCATAGTTACCAACTTTATGGGAACTCTCAACTTGCTTGGCCTTCTTGGTGGTTTTCTAGCTGATGCAAAACTTGGCAGATACTTAACTGTTGCCATATCTGCAACCATAGCCACAGTGGGTGTGTGTATGTTAACTTTGGCTACAACAATTCCTAGTATGACACCTCCACCATGCAGTGAAGTAAGAAGACAACATCATCAGTGCATTCAAGCAAGTGGCAAACAGTTAGCACTTCTCTTTGCAGCACTTTATACAATAGCTTTAGGTGGTGGAGGAATAAAATCCAATGTGTCAGGTTTTGGATCTGATCAATTTGACACAAATGATCCAAAGGAAGAGAAGAATATGATATTTTTCTTCAACAGATTCTACTTTTTTATAAGTATTGGATCCTTATTTTCAGTGGTTGTATTGGTTTATGTTCAAGACAATGTAGGAAGAGGTTGGGGATATGGAATTTCAGCAGGGACAATGTTGGTAGCAAAGAGGACTCTTCCTATCCCTTCTGATCCTACCTTACTCAATGGTTATCTTGAAGCTAAGGTCACATATACAGACAAATTCAGGTACCTTGACAAAGCAGCAATCCTAGATGAAACAAACTCAAAGGACGGAAACAACGAAAATCCATGGTTAGTTTCAACAATGACTCAAGTTGAGGAAGTTAAAATGGTAATCAAACTCCTTCCCATTTGGTCAACATGCATACTCTTTTGGACAGTTTATTCTCAAATGAACACCTTCACAATTGAGCAAGCTACATTCATGAACAGAAAAGTTGGATCACTAGAGATTCCAGCAGGATCCTTATCAGCTTTTCTCATCATCACCATTCTCCTCTTTACTTCCCTAAATGAGAAAAATAACTTTTCTGCTATCGTTGAGAAAGAAAGAAGGGACAATGCAGTTAAAAAACAAACTACAATAAGTGCTTTTTGGCTTGTACCTCAGTTTTTTCTAGTTGGTGCTGGAGAAGCTTTTGCTTATGTTGGACAGTTAGAGTTTTTCATAAGGGAAGCACCTGAGAGGATGAAATCTATGAGTACTGGTCTTTTTCTATGTACACTTTCAATGGGATATTTTGTTAGTAGCTTATTGGTTTCAATTGTGGACAAAGTAAGCAAGAAAAGATGGTTGAAGAGTAATCTTGACAAGGACTCTGATATTTCCAAATCTGTTGAGATTTGCAGGTTTCAAGTCATCTTGTATGCCTACACTACGGTACTTTGTGCTCGAGAGAGCCTTTATGCTTGCTGCTTGATTCAGGGAGCTGTCGGCACTTGGATTTGTTACCGTGTTGGCGGTCGATCCATTCGCTGA
Protein sequence

>Msa0782330

MVLVASNGEKKGTEDNAAVDFRGHPADKTKTGGWLAAGLILGTELAERICVMGISMNLVTYLVGDLHLHSASSATIVTNFMGTLNLLGLLGGFLADAKLGRYLTVAISATIATVGVCMLTLATTIPSMTPPPCSEVRRQHHQCIQASGKQLALLFAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKNMIFFFNRFYFFISIGSLFSVVVLVYVQDNVGRGWGYGISAGTMLVAKRTLPIPSDPTLLNGYLEAKVTYTDKFRYLDKAAILDETNSKDGNNENPWLVSTMTQVEEVKMVIKLLPIWSTCILFWTVYSQMNTFTIEQATFMNRKVGSLEIPAGSLSAFLIITILLFTSLNEKNNFSAIVEKERRDNAVKKQTTISAFWLVPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLCTLSMGYFVSSLLVSIVDKVSKKRWLKSNLDKDSDISKSVEICRFQVILYAYTTVLCARESLYACCLIQGAVGTWICYRVGGRSIR*