AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0652410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652410 MtrunA17_Chr4g0046091 95.455 286 9 2 1 282 1 286 0.0 550
Msa0652410 MtrunA17_Chr2g0283341 50.644 233 86 4 27 259 2 205 6.77e-76 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652410 AT1G28960.3 70.775 284 77 4 3 281 10 292 4.79e-135 384
Msa0652410 AT1G28960.1 70.775 284 77 4 3 281 10 292 4.79e-135 384
Msa0652410 AT1G28960.5 70.775 284 77 4 3 281 10 292 4.79e-135 384
Msa0652410 AT1G28960.7 71.480 277 73 4 3 274 10 285 4.71e-133 378
Msa0652410 AT1G28960.6 71.480 277 73 4 3 274 10 285 4.71e-133 378
Msa0652410 AT2G33980.2 68.421 247 71 3 30 274 18 259 2.18e-115 332
Msa0652410 AT2G33980.4 68.421 247 71 3 30 274 18 259 2.18e-115 332
Msa0652410 AT2G33980.1 68.421 247 71 3 30 274 18 259 8.61e-115 332
Msa0652410 AT2G33980.3 68.421 247 71 3 30 274 18 259 1.36e-114 333
Msa0652410 AT5G45940.1 61.376 189 69 2 85 269 29 217 8.94e-79 238
Msa0652410 AT5G45940.2 63.281 128 44 1 85 209 29 156 5.06e-51 165
Msa0652410 AT5G45940.3 64.286 126 42 1 85 207 29 154 1.30e-50 164

Find 99 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTCTTCCTTTCTTTC+AGG 0.144264 4_3:+60122102 None:intergenic
AACCTGTGGAACTTTGAATT+TGG 0.148694 4_3:+60122244 None:intergenic
TGAATCATGGTAGCTGATTC+AGG 0.246118 4_3:+60124560 None:intergenic
GTAACAACATTGACTAGTTT+TGG 0.246703 4_3:+60123992 None:intergenic
TTCTTAGGACTAAATTTATC+TGG 0.253323 4_3:+60124533 None:intergenic
CGAGAGGCAGAAGAGGAAAT+TGG 0.270083 4_3:-60124022 Msa0652410:CDS
TCTTGAACCATTCTTGTCTA+AGG 0.274255 4_3:-60123966 Msa0652410:intron
CTCTGTTCCAAAACATCTTC+AGG 0.287071 4_3:+60124626 None:intergenic
ATTTCTGCTCACCTTGTGTT+TGG 0.302067 4_3:-60122058 Msa0652410:three_prime_UTR
TGGCAAAGTTGTTTCTCAAT+TGG 0.304222 4_3:-60124588 Msa0652410:CDS
ATCAAAACAGCAGCTTTCTT+AGG 0.317340 4_3:+60124518 None:intergenic
ACCTGTGGAACTTTGAATTT+GGG 0.326845 4_3:+60122245 None:intergenic
GCAAACTGCCATCACATCTA+TGG 0.329294 4_3:+60121865 None:intergenic
CAATAACAATTTGATACAAT+AGG 0.347970 4_3:-60124851 Msa0652410:five_prime_UTR
GGCAAAGTTGTTTCTCAATT+GGG 0.351294 4_3:-60124587 Msa0652410:CDS
AAGGTGATGATGGGGATCTA+AGG 0.355981 4_3:-60124484 Msa0652410:CDS
TTGGTGACAGATACTGTTCT+TGG 0.364656 4_3:+60124737 None:intergenic
ACTAGCAGTTTAATGTTTGA+AGG 0.371560 4_3:+60124911 None:intergenic
CGATGACAGGAACTACTCTT+AGG 0.397188 4_3:+60122624 None:intergenic
TCCAGATCCCAAAATTAGTC+TGG 0.402451 4_3:+60121983 None:intergenic
TGCAGCCCTAATCAAAATTC+CGG 0.409951 4_3:+60122310 None:intergenic
TCAGGAAGAGAGAGAGTGGA+TGG 0.414184 4_3:-60122408 Msa0652410:CDS
TAGAATCAATCAAATGGAAA+AGG 0.418865 4_3:-60122020 Msa0652410:three_prime_UTR
TGCTAGTTTAACTACTTGTC+CGG 0.424659 4_3:-60124895 Msa0652410:five_prime_UTR
CCTTCCTCCGCTTTCCCACC+AGG 0.426104 4_3:+60124073 None:intergenic
TACAATAATATATATTCGTC+AGG 0.427184 4_3:-60122141 Msa0652410:three_prime_UTR
AGAATCAATCAAATGGAAAA+GGG 0.434148 4_3:-60122019 Msa0652410:three_prime_UTR
TTGCAAAAGCTAATCTAAAC+CGG 0.444277 4_3:+60124876 None:intergenic
GAAAGCGGAGGAAGGGGATA+AGG 0.452799 4_3:-60124065 Msa0652410:CDS
GATCGGTAGAATCAATCAAA+TGG 0.459510 4_3:-60122026 Msa0652410:three_prime_UTR
TGTTTATTTGAAGGTGATGA+TGG 0.461797 4_3:-60124494 Msa0652410:CDS
AATAACAATTTGATACAATA+GGG 0.463090 4_3:-60124850 Msa0652410:five_prime_UTR
ACCTGAGTGAGTAGAGAGCT+TGG 0.464566 4_3:+60124439 None:intergenic
AATACTGATTCCACTTCTGC+AGG 0.466410 4_3:+60122560 None:intergenic
ACTTCTGCAGGATTCAGAAC+AGG 0.470641 4_3:+60122572 None:intergenic
TGGTGGGAAAGCGGAGGAAG+GGG 0.473889 4_3:-60124071 Msa0652410:CDS
GCTTTAAGCAGGATGAAAAT+CGG 0.478548 4_3:-60122433 Msa0652410:intron
TTTGAAGGTCTGATCCTTGT+CGG 0.479051 4_3:+60124926 None:intergenic
CCTGGTGGGAAAGCGGAGGA+AGG 0.480967 4_3:-60124073 Msa0652410:CDS
TCCAAAACATCTTCAGGAAA+AGG 0.484289 4_3:+60124632 None:intergenic
GGAATAATAAAATGTTGTAA+AGG 0.484974 4_3:-60121963 Msa0652410:three_prime_UTR
ATTTATATCTCCTGAAAGAA+AGG 0.485574 4_3:-60122112 Msa0652410:three_prime_UTR
GAAATATGAATTCCAAGTGA+TGG 0.489702 4_3:+60122076 None:intergenic
GAGAGGCAGAAGAGGAAATT+GGG 0.494487 4_3:-60124021 Msa0652410:CDS
TGTTCTGAATCCTGCAGAAG+TGG 0.496762 4_3:-60122570 Msa0652410:CDS
CTGGTGGGAAAGCGGAGGAA+GGG 0.497359 4_3:-60124072 Msa0652410:CDS
GCAGGATGAAAATCGGAGTC+AGG 0.500021 4_3:-60122426 Msa0652410:intron
ACCTTGAGAAACATTTCTAA+AGG 0.503154 4_3:+60122530 None:intergenic
TCCCAAATTCAAAGTTCCAC+AGG 0.509189 4_3:-60122246 Msa0652410:CDS
CTGCATCAATTGTGTACAAA+CGG 0.513281 4_3:-60122292 Msa0652410:CDS
CTCATATGGGGATTAACTGC+CGG 0.518356 4_3:-60122329 Msa0652410:CDS
GGAAGAAGAATCCATGACTT+TGG 0.522178 4_3:+60124718 None:intergenic
ATCTCCTGAAAGAAAGGAAG+AGG 0.524063 4_3:-60122106 Msa0652410:three_prime_UTR
GGCAGAAGAGGAAATTGGGT+TGG 0.528513 4_3:-60124017 Msa0652410:CDS
ATGGATTCTTCTTCCAGTGG+TGG 0.530689 4_3:-60124710 Msa0652410:CDS
TCATGAAGTATGCCGATGAC+AGG 0.534330 4_3:+60122611 None:intergenic
AGAGGATGCTGTCCATCACT+TGG 0.536088 4_3:-60122088 Msa0652410:three_prime_UTR
CAGGAAGAGAGAGAGTGGAT+GGG 0.539082 4_3:-60122407 Msa0652410:CDS
TGAAGAAAAGAAATATATTG+AGG 0.545140 4_3:+60122167 None:intergenic
GTTTATTTGAAGGTGATGAT+GGG 0.545292 4_3:-60124493 Msa0652410:CDS
GTGTTTGGATATCTAATGAT+CGG 0.547839 4_3:-60122043 Msa0652410:three_prime_UTR
ACCTTTAGAAATGTTTCTCA+AGG 0.548045 4_3:-60122531 Msa0652410:intron
GGATTCTGCTCTATGAAAGC+CGG 0.548477 4_3:+60122266 None:intergenic
AGGTGATGATGGGGATCTAA+GGG 0.549052 4_3:-60124483 Msa0652410:CDS
TGCAAAACGAGAGGCAGAAG+AGG 0.549211 4_3:-60124029 Msa0652410:CDS
ATCTCAGAGACTTCTTGCGT+TGG 0.549765 4_3:-60124687 Msa0652410:CDS
ATTTGATACAATAGGGTAGG+TGG 0.554437 4_3:-60124843 Msa0652410:five_prime_UTR
AGTTCCACAGGTTGTAAGCA+AGG 0.560370 4_3:-60122234 Msa0652410:CDS
CTATCCTTGCTTACAACCTG+TGG 0.562710 4_3:+60122230 None:intergenic
ATAGATACCTTAGACAAGAA+TGG 0.563801 4_3:+60123959 None:intergenic
AATTGTGTACAAACGGCCAC+CGG 0.564192 4_3:-60122285 Msa0652410:CDS
TCTTCAGGAAAAGGTGGTGG+TGG 0.564320 4_3:+60124641 None:intergenic
GTCATTGCCTGGTGGGAAAG+CGG 0.565959 4_3:-60124080 Msa0652410:CDS
TGAAATGATAAGCAATAAGA+TGG 0.579545 4_3:-60121925 Msa0652410:three_prime_UTR
AGTATCTGTCACCAAAGTCA+TGG 0.580073 4_3:-60124729 Msa0652410:CDS
CTAAGAGTAGTTCCTGTCAT+CGG 0.582497 4_3:-60122623 Msa0652410:CDS
GGAGTCAGGAAGAGAGAGAG+TGG 0.584588 4_3:-60122412 Msa0652410:CDS
GTAGGTGAAGTGTCATTGCC+TGG 0.586937 4_3:-60124091 Msa0652410:intron
AGGATAGTTCAATGACTTGA+CGG 0.587268 4_3:-60122214 Msa0652410:exon
CGCGGCCACTGCAAAACGAG+AGG 0.591310 4_3:-60124038 Msa0652410:CDS
ACATCTTCAGGAAAAGGTGG+TGG 0.591697 4_3:+60124638 None:intergenic
TCCAAGCTCTCTACTCACTC+AGG 0.594205 4_3:-60124440 Msa0652410:intron
ACAATTTGATACAATAGGGT+AGG 0.595581 4_3:-60124846 Msa0652410:five_prime_UTR
TTTGATACAATAGGGTAGGT+GGG 0.596155 4_3:-60124842 Msa0652410:five_prime_UTR
GTCATGGATTCTTCTTCCAG+TGG 0.604832 4_3:-60124713 Msa0652410:CDS
TGACAGGAACTACTCTTAGG+AGG 0.607814 4_3:+60122627 None:intergenic
AAGTCTCTGAGATCCACCAC+TGG 0.613439 4_3:+60124697 None:intergenic
GAATCAATCAAATGGAAAAG+GGG 0.620781 4_3:-60122018 Msa0652410:three_prime_UTR
TTTATTTGAAGGTGATGATG+GGG 0.628042 4_3:-60124492 Msa0652410:CDS
AAAACATCTTCAGGAAAAGG+TGG 0.635918 4_3:+60124635 None:intergenic
GAACAGAGCATTGAAGAGAG+TGG 0.639203 4_3:-60124611 Msa0652410:CDS
TTCTGCTCTATGAAAGCCGG+TGG 0.643205 4_3:+60122269 None:intergenic
GTGAAGTGTCATTGCCTGGT+GGG 0.658192 4_3:-60124087 Msa0652410:CDS
ATTGCCTGGTGGGAAAGCGG+AGG 0.658525 4_3:-60124077 Msa0652410:CDS
ACAAAGATTTCATTCCGACA+AGG 0.669608 4_3:-60124940 Msa0652410:five_prime_UTR
CAGAGCATTGAAGAGAGTGG+TGG 0.680698 4_3:-60124608 Msa0652410:CDS
GGTGAAGTGTCATTGCCTGG+TGG 0.686704 4_3:-60124088 Msa0652410:CDS
TCATTAGATATCCAAACACA+AGG 0.698944 4_3:+60122047 None:intergenic
GGAAGGGGATAAGGACGACG+CGG 0.736631 4_3:-60124056 Msa0652410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATCATTTTAAGAAAA+GGG + chr4_3:60123407-60123426 None:intergenic 10.0%
!!! TTATAAATCATTTTAAGAAA+AGG + chr4_3:60123408-60123427 None:intergenic 10.0%
!! AATAACAATTTGATACAATA+GGG - chr4_3:60121956-60121975 Msa0652410:three_prime_UTR 15.0%
!!! TTTTCTTTAATAGGAAAAAA+AGG + chr4_3:60123561-60123580 None:intergenic 15.0%
!!! AATCAATTTGAGAAATTTTT+CGG - chr4_3:60123877-60123896 Msa0652410:intron 15.0%
!! CAATAACAATTTGATACAAT+AGG - chr4_3:60121955-60121974 Msa0652410:three_prime_UTR 20.0%
!!! GTTTTGATTTGTTTATTTGA+AGG - chr4_3:60122303-60122322 Msa0652410:CDS 20.0%
!!! TTTTTTTAGACAAATGTTAG+TGG - chr4_3:60122469-60122488 Msa0652410:intron 20.0%
!!! TACTTTAGTTATTGTGATTT+TGG - chr4_3:60122636-60122655 Msa0652410:intron 20.0%
!!! TTTTGGTTATTTGAATTTAG+AGG - chr4_3:60122653-60122672 Msa0652410:intron 20.0%
!! TTTGGTTATTTGAATTTAGA+GGG - chr4_3:60122654-60122673 Msa0652410:intron 20.0%
!!! TTTCAGCAATTTTCTTTAAT+AGG + chr4_3:60123570-60123589 None:intergenic 20.0%
!!! TTTTTCGGAAATTTATTTGT+TGG - chr4_3:60123892-60123911 Msa0652410:intron 20.0%
!!! GAAATTTATTTGTTGGATAA+TGG - chr4_3:60123899-60123918 Msa0652410:intron 20.0%
!! GGGTTATAAATTTGTATTAT+TGG - chr4_3:60124014-60124033 Msa0652410:CDS 20.0%
!! TGAAGAAAAGAAATATATTG+AGG + chr4_3:60124642-60124661 None:intergenic 20.0%
!! TACAATAATATATATTCGTC+AGG - chr4_3:60124665-60124684 Msa0652410:CDS 20.0%
!!! AAAAAATTCCAGACTAATTT+TGG - chr4_3:60124815-60124834 Msa0652410:five_prime_UTR 20.0%
!!! AAAAATTCCAGACTAATTTT+GGG - chr4_3:60124816-60124835 Msa0652410:five_prime_UTR 20.0%
!! GGAATAATAAAATGTTGTAA+AGG - chr4_3:60124843-60124862 Msa0652410:five_prime_UTR 20.0%
!! TCTTTGTCTTAGATTTTACA+TGG + chr4_3:60121853-60121872 None:intergenic 25.0%
!! TTTATCTGGATTTTGAATCA+TGG + chr4_3:60122262-60122281 None:intergenic 25.0%
! TTCTTAGGACTAAATTTATC+TGG + chr4_3:60122276-60122295 None:intergenic 25.0%
! TAAAGATTCAAAATTCCAAG+TGG + chr4_3:60122924-60122943 None:intergenic 25.0%
!!! CTAGAAAATGTTGATTTTGT+TGG - chr4_3:60122992-60123011 Msa0652410:intron 25.0%
!! TGTAATTATTTAGCTCATGA+AGG - chr4_3:60123035-60123054 Msa0652410:intron 25.0%
! ACTTCATTCTTGAATATCAT+CGG - chr4_3:60123177-60123196 Msa0652410:intron 25.0%
! AATTCCTGAAAACAATAAAC+AGG + chr4_3:60123444-60123463 None:intergenic 25.0%
! GATACAAATAAAACTAAGCA+TGG + chr4_3:60124303-60124322 None:intergenic 25.0%
! AGCAGAAAAAATATCTCATA+TGG - chr4_3:60124463-60124482 Msa0652410:CDS 25.0%
! GCAGAAAAAATATCTCATAT+GGG - chr4_3:60124464-60124483 Msa0652410:CDS 25.0%
! CAGAAAAAATATCTCATATG+GGG - chr4_3:60124465-60124484 Msa0652410:CDS 25.0%
! ATTTATATCTCCTGAAAGAA+AGG - chr4_3:60124694-60124713 Msa0652410:CDS 25.0%
! TAGAATCAATCAAATGGAAA+AGG - chr4_3:60124786-60124805 Msa0652410:exon 25.0%
! AGAATCAATCAAATGGAAAA+GGG - chr4_3:60124787-60124806 Msa0652410:exon 25.0%
! TGAAATGATAAGCAATAAGA+TGG - chr4_3:60124881-60124900 Msa0652410:five_prime_UTR 25.0%
!! TTTTTAATCCATAGATGTGA+TGG - chr4_3:60124933-60124952 Msa0652410:five_prime_UTR 25.0%
ACTAGCAGTTTAATGTTTGA+AGG + chr4_3:60121898-60121917 None:intergenic 30.0%
TTGCAAAAGCTAATCTAAAC+CGG + chr4_3:60121933-60121952 None:intergenic 30.0%
ACAATTTGATACAATAGGGT+AGG - chr4_3:60121960-60121979 Msa0652410:three_prime_UTR 30.0%
TGTTTATTTGAAGGTGATGA+TGG - chr4_3:60122312-60122331 Msa0652410:CDS 30.0%
GTTTATTTGAAGGTGATGAT+GGG - chr4_3:60122313-60122332 Msa0652410:CDS 30.0%
! TTTATTTGAAGGTGATGATG+GGG - chr4_3:60122314-60122333 Msa0652410:CDS 30.0%
AATTTAGAGGGAATGAATTG+AGG - chr4_3:60122666-60122685 Msa0652410:intron 30.0%
ATTTAGAGGGAATGAATTGA+GGG - chr4_3:60122667-60122686 Msa0652410:intron 30.0%
!!! GTAACAACATTGACTAGTTT+TGG + chr4_3:60122817-60122836 None:intergenic 30.0%
ATAGATACCTTAGACAAGAA+TGG + chr4_3:60122850-60122869 None:intergenic 30.0%
TTAATTGAGTATTCTCCACT+TGG - chr4_3:60122906-60122925 Msa0652410:intron 30.0%
AAATTTATGTCTGCGATTGA+TGG - chr4_3:60122946-60122965 Msa0652410:intron 30.0%
CCAAGACAACAATCATAATA+AGG + chr4_3:60123290-60123309 None:intergenic 30.0%
CCTTATTATGATTGTTGTCT+TGG - chr4_3:60123287-60123306 Msa0652410:intron 30.0%
! CTATCCTGTTTATTGTTTTC+AGG - chr4_3:60123437-60123456 Msa0652410:intron 30.0%
! CTAATCAGTTTGTTTACATC+AGG + chr4_3:60123471-60123490 None:intergenic 30.0%
CTGATGTAAACAAACTGATT+AGG - chr4_3:60123469-60123488 Msa0652410:intron 30.0%
CTAAGAAATGTTGCATTCAA+TGG - chr4_3:60123525-60123544 Msa0652410:intron 30.0%
AAAGCATAAGTTCGTTATTG+TGG + chr4_3:60123669-60123688 None:intergenic 30.0%
! TTATGCTTTGAACTAGATAG+AGG - chr4_3:60123680-60123699 Msa0652410:intron 30.0%
TAACAACACTCCATAGATTT+AGG - chr4_3:60123792-60123811 Msa0652410:intron 30.0%
!!! TGCATAAAGTTTTGAAACTG+TGG + chr4_3:60123925-60123944 None:intergenic 30.0%
GAGGAATAGACTAATAGAAA+AGG + chr4_3:60124080-60124099 None:intergenic 30.0%
GCTGTAAAAGAAACAATGAT+AGG + chr4_3:60124158-60124177 None:intergenic 30.0%
! ACCTTTAGAAATGTTTCTCA+AGG - chr4_3:60124275-60124294 Msa0652410:intron 30.0%
ACCTTGAGAAACATTTCTAA+AGG + chr4_3:60124279-60124298 None:intergenic 30.0%
GAAATATGAATTCCAAGTGA+TGG + chr4_3:60124733-60124752 None:intergenic 30.0%
TCATTAGATATCCAAACACA+AGG + chr4_3:60124762-60124781 None:intergenic 30.0%
GTGTTTGGATATCTAATGAT+CGG - chr4_3:60124763-60124782 Msa0652410:CDS 30.0%
GAATCAATCAAATGGAAAAG+GGG - chr4_3:60124788-60124807 Msa0652410:exon 30.0%
AAGATGGTGAAAAATGAACT+TGG - chr4_3:60124897-60124916 Msa0652410:five_prime_UTR 30.0%
ACAAAGATTTCATTCCGACA+AGG - chr4_3:60121866-60121885 Msa0652410:three_prime_UTR 35.0%
! TGCTAGTTTAACTACTTGTC+CGG - chr4_3:60121911-60121930 Msa0652410:three_prime_UTR 35.0%
ATTTGATACAATAGGGTAGG+TGG - chr4_3:60121963-60121982 Msa0652410:three_prime_UTR 35.0%
TTTGATACAATAGGGTAGGT+GGG - chr4_3:60121964-60121983 Msa0652410:three_prime_UTR 35.0%
AAAACATCTTCAGGAAAAGG+TGG + chr4_3:60122174-60122193 None:intergenic 35.0%
! ACCTTTTCCTGAAGATGTTT+TGG - chr4_3:60122173-60122192 Msa0652410:three_prime_UTR 35.0%
TCCAAAACATCTTCAGGAAA+AGG + chr4_3:60122177-60122196 None:intergenic 35.0%
!! TGGCAAAGTTGTTTCTCAAT+TGG - chr4_3:60122218-60122237 Msa0652410:CDS 35.0%
!! GGCAAAGTTGTTTCTCAATT+GGG - chr4_3:60122219-60122238 Msa0652410:CDS 35.0%
ATCAAAACAGCAGCTTTCTT+AGG + chr4_3:60122291-60122310 None:intergenic 35.0%
GCACATACACTTCTCATATT+AGG + chr4_3:60122541-60122560 None:intergenic 35.0%
AACCATTTGCCCAATAGTAT+AGG + chr4_3:60122598-60122617 None:intergenic 35.0%
ATACTATTGGGCAAATGGTT+TGG - chr4_3:60122598-60122617 Msa0652410:CDS 35.0%
TACTATTGGGCAAATGGTTT+GGG - chr4_3:60122599-60122618 Msa0652410:CDS 35.0%
TCTTGAACCATTCTTGTCTA+AGG - chr4_3:60122840-60122859 Msa0652410:intron 35.0%
GTCTGTATAAAGTAATGCAG+TGG - chr4_3:60123335-60123354 Msa0652410:intron 35.0%
GGGTATGTATCTGATTCTTT+AGG + chr4_3:60123387-60123406 None:intergenic 35.0%
! TACATGACTTTTACAGTTGG+TGG + chr4_3:60123607-60123626 None:intergenic 35.0%
! GGATACATGACTTTTACAGT+TGG + chr4_3:60123610-60123629 None:intergenic 35.0%
AAAAGTCATGTATCCATTGC+AGG - chr4_3:60123615-60123634 Msa0652410:intron 35.0%
GTTATGCTGTCCTAAATCTA+TGG + chr4_3:60123805-60123824 None:intergenic 35.0%
ACGTTCTTATTAGCAACGAT+TGG - chr4_3:60123847-60123866 Msa0652410:intron 35.0%
AAGTATGACCATTTCAGAGT+CGG - chr4_3:60123980-60123999 Msa0652410:CDS 35.0%
ATGTTTACATTCCCTGTTGT+TGG - chr4_3:60124042-60124061 Msa0652410:CDS 35.0%
GTGTAAACTATCCAACAACA+GGG + chr4_3:60124056-60124075 None:intergenic 35.0%
TGTGTAAACTATCCAACAAC+AGG + chr4_3:60124057-60124076 None:intergenic 35.0%
! GCTTTAAGCAGGATGAAAAT+CGG - chr4_3:60124373-60124392 Msa0652410:intron 35.0%
!!! TAACTGCCGGAATTTTGATT+AGG - chr4_3:60124490-60124509 Msa0652410:CDS 35.0%
!!! AACTGCCGGAATTTTGATTA+GGG - chr4_3:60124491-60124510 Msa0652410:CDS 35.0%
CTGCATCAATTGTGTACAAA+CGG - chr4_3:60124514-60124533 Msa0652410:CDS 35.0%
ACCTGTGGAACTTTGAATTT+GGG + chr4_3:60124564-60124583 None:intergenic 35.0%
AACCTGTGGAACTTTGAATT+TGG + chr4_3:60124565-60124584 None:intergenic 35.0%
AGGATAGTTCAATGACTTGA+CGG - chr4_3:60124592-60124611 Msa0652410:CDS 35.0%
GATCGGTAGAATCAATCAAA+TGG - chr4_3:60124780-60124799 Msa0652410:exon 35.0%
TTTGAAGGTCTGATCCTTGT+CGG + chr4_3:60121883-60121902 None:intergenic 40.0%
! TTGGTGACAGATACTGTTCT+TGG + chr4_3:60122072-60122091 None:intergenic 40.0%
AGTATCTGTCACCAAAGTCA+TGG - chr4_3:60122077-60122096 Msa0652410:three_prime_UTR 40.0%
GGAAGAAGAATCCATGACTT+TGG + chr4_3:60122091-60122110 None:intergenic 40.0%
CTCTGTTCCAAAACATCTTC+AGG + chr4_3:60122183-60122202 None:intergenic 40.0%
! TGAATCATGGTAGCTGATTC+AGG + chr4_3:60122249-60122268 None:intergenic 40.0%
TTTGCCCAATAGTATAGGCT+GGG + chr4_3:60122593-60122612 None:intergenic 40.0%
ATTTGCCCAATAGTATAGGC+TGG + chr4_3:60122594-60122613 None:intergenic 40.0%
AGCCTATACTATTGGGCAAA+TGG - chr4_3:60122593-60122612 Msa0652410:CDS 40.0%
ACTATTGGGCAAATGGTTTG+GGG - chr4_3:60122600-60122619 Msa0652410:CDS 40.0%
GGTGTGTGTGTTGTATATGT+AGG - chr4_3:60122697-60122716 Msa0652410:intron 40.0%
! TGCGATTGATGGTTAGTTCA+TGG - chr4_3:60122957-60122976 Msa0652410:intron 40.0%
TACTTATTCCTCACACAGCA+TGG + chr4_3:60123066-60123085 None:intergenic 40.0%
TAAAGTAATGCAGTGGCCAT+TGG - chr4_3:60123342-60123361 Msa0652410:intron 40.0%
TCCATTGCAGGTCATTAAAC+TGG - chr4_3:60123627-60123646 Msa0652410:intron 40.0%
CCATTGCAGGTCATTAAACT+GGG - chr4_3:60123628-60123647 Msa0652410:intron 40.0%
ACTTAGTGCTCAGCATAAAC+AGG - chr4_3:60123752-60123771 Msa0652410:intron 40.0%
AAATTTATAACCCGTGACCG+CGG + chr4_3:60124007-60124026 None:intergenic 40.0%
AGTCTATTCCTCATCTGCTT+CGG - chr4_3:60124088-60124107 Msa0652410:CDS 40.0%
!! CGGATTGAAAGTGTTCAATG+AGG - chr4_3:60124108-60124127 Msa0652410:intron 40.0%
TGATAGGTGAGATGAAGTAC+TGG + chr4_3:60124142-60124161 None:intergenic 40.0%
CTAAGAGTAGTTCCTGTCAT+CGG - chr4_3:60124183-60124202 Msa0652410:intron 40.0%
!! AATACTGATTCCACTTCTGC+AGG + chr4_3:60124249-60124268 None:intergenic 40.0%
TGCAGCCCTAATCAAAATTC+CGG + chr4_3:60124499-60124518 None:intergenic 40.0%
TCCCAAATTCAAAGTTCCAC+AGG - chr4_3:60124560-60124579 Msa0652410:CDS 40.0%
ATCTCCTGAAAGAAAGGAAG+AGG - chr4_3:60124700-60124719 Msa0652410:CDS 40.0%
ATTTCTGCTCACCTTGTGTT+TGG - chr4_3:60124748-60124767 Msa0652410:CDS 40.0%
!!! TCCAGACTAATTTTGGGATC+TGG - chr4_3:60124822-60124841 Msa0652410:five_prime_UTR 40.0%
TCCAGATCCCAAAATTAGTC+TGG + chr4_3:60124826-60124845 None:intergenic 40.0%
GTCATGGATTCTTCTTCCAG+TGG - chr4_3:60122093-60122112 Msa0652410:three_prime_UTR 45.0%
ATGGATTCTTCTTCCAGTGG+TGG - chr4_3:60122096-60122115 Msa0652410:three_prime_UTR 45.0%
ATCTCAGAGACTTCTTGCGT+TGG - chr4_3:60122119-60122138 Msa0652410:three_prime_UTR 45.0%
ACATCTTCAGGAAAAGGTGG+TGG + chr4_3:60122171-60122190 None:intergenic 45.0%
!! GAACAGAGCATTGAAGAGAG+TGG - chr4_3:60122195-60122214 Msa0652410:three_prime_UTR 45.0%
!! AAGGTGATGATGGGGATCTA+AGG - chr4_3:60122322-60122341 Msa0652410:CDS 45.0%
!! AGGTGATGATGGGGATCTAA+GGG - chr4_3:60122323-60122342 Msa0652410:CDS 45.0%
AGATAACGTGTGTCTGACAC+AGG - chr4_3:60122509-60122528 Msa0652410:intron 45.0%
GATAACGTGTGTCTGACACA+GGG - chr4_3:60122510-60122529 Msa0652410:intron 45.0%
TGTGTCCCAGCCTATACTAT+TGG - chr4_3:60122585-60122604 Msa0652410:CDS 45.0%
GTGTCCCAGCCTATACTATT+GGG - chr4_3:60122586-60122605 Msa0652410:CDS 45.0%
! CTATTGGGCAAATGGTTTGG+GGG - chr4_3:60122601-60122620 Msa0652410:CDS 45.0%
AGGGAATGAATTGAGGGTTG+TGG - chr4_3:60122673-60122692 Msa0652410:intron 45.0%
GAATGAATTGAGGGTTGTGG+TGG - chr4_3:60122676-60122695 Msa0652410:intron 45.0%
GAGAGGCAGAAGAGGAAATT+GGG - chr4_3:60122785-60122804 Msa0652410:intron 45.0%
! TGCTCTGAGTCTGAGATGAT+TGG - chr4_3:60123093-60123112 Msa0652410:intron 45.0%
! GAGTCTGAGATGATTGGAGA+TGG - chr4_3:60123099-60123118 Msa0652410:intron 45.0%
! TCTGAGATGATTGGAGATGG+AGG - chr4_3:60123102-60123121 Msa0652410:intron 45.0%
CTGAGATGATTGGAGATGGA+GGG - chr4_3:60123103-60123122 Msa0652410:intron 45.0%
TCAGCTGCACTTCATGTATC+TGG + chr4_3:60123243-60123262 None:intergenic 45.0%
AGATACATGAAGTGCAGCTG+AGG - chr4_3:60123242-60123261 Msa0652410:intron 45.0%
ATCCCATTCCCATTCACCAA+TGG + chr4_3:60123361-60123380 None:intergenic 45.0%
TTCTTCACTGTCCACGTTCA+TGG - chr4_3:60123493-60123512 Msa0652410:intron 45.0%
AGTACGTACAACCATGAACG+TGG + chr4_3:60123507-60123526 None:intergenic 45.0%
CCCAGTTTAATGACCTGCAA+TGG + chr4_3:60123631-60123650 None:intergenic 45.0%
CTTTCAATCCGAAGCAGATG+AGG + chr4_3:60124099-60124118 None:intergenic 45.0%
TGACAGGAACTACTCTTAGG+AGG + chr4_3:60124182-60124201 None:intergenic 45.0%
CGATGACAGGAACTACTCTT+AGG + chr4_3:60124185-60124204 None:intergenic 45.0%
TCATGAAGTATGCCGATGAC+AGG + chr4_3:60124198-60124217 None:intergenic 45.0%
ACTTCTGCAGGATTCAGAAC+AGG + chr4_3:60124237-60124256 None:intergenic 45.0%
! TGTTCTGAATCCTGCAGAAG+TGG - chr4_3:60124236-60124255 Msa0652410:intron 45.0%
! CTGAAGCGATTGCTTTAAGC+AGG - chr4_3:60124362-60124381 Msa0652410:intron 45.0%
CTCATATGGGGATTAACTGC+CGG - chr4_3:60124477-60124496 Msa0652410:CDS 45.0%
AATTGTGTACAAACGGCCAC+CGG - chr4_3:60124521-60124540 Msa0652410:CDS 45.0%
GGATTCTGCTCTATGAAAGC+CGG + chr4_3:60124543-60124562 None:intergenic 45.0%
AGTTCCACAGGTTGTAAGCA+AGG - chr4_3:60124572-60124591 Msa0652410:CDS 45.0%
CTATCCTTGCTTACAACCTG+TGG + chr4_3:60124579-60124598 None:intergenic 45.0%
GCATCCTCTTCCTTTCTTTC+AGG + chr4_3:60124707-60124726 None:intergenic 45.0%
GCAAACTGCCATCACATCTA+TGG + chr4_3:60124944-60124963 None:intergenic 45.0%
!! AAGTCTCTGAGATCCACCAC+TGG + chr4_3:60122112-60122131 None:intergenic 50.0%
! TCTTCAGGAAAAGGTGGTGG+TGG + chr4_3:60122168-60122187 None:intergenic 50.0%
!! CAGAGCATTGAAGAGAGTGG+TGG - chr4_3:60122198-60122217 Msa0652410:exon 50.0%
TCCAAGCTCTCTACTCACTC+AGG - chr4_3:60122366-60122385 Msa0652410:CDS 50.0%
ACCTGAGTGAGTAGAGAGCT+TGG + chr4_3:60122370-60122389 None:intergenic 50.0%
! GTAGGTGAAGTGTCATTGCC+TGG - chr4_3:60122715-60122734 Msa0652410:intron 50.0%
! GTGAAGTGTCATTGCCTGGT+GGG - chr4_3:60122719-60122738 Msa0652410:intron 50.0%
! TTCTGCCTCTCGTTTTGCAG+TGG + chr4_3:60122776-60122795 None:intergenic 50.0%
TGCAAAACGAGAGGCAGAAG+AGG - chr4_3:60122777-60122796 Msa0652410:intron 50.0%
CGAGAGGCAGAAGAGGAAAT+TGG - chr4_3:60122784-60122803 Msa0652410:intron 50.0%
GGCAGAAGAGGAAATTGGGT+TGG - chr4_3:60122789-60122808 Msa0652410:intron 50.0%
AGGAGATTCCATGCTGTGTG+AGG - chr4_3:60123055-60123074 Msa0652410:intron 50.0%
ATGCAGTGGCCATTGGTGAA+TGG - chr4_3:60123349-60123368 Msa0652410:intron 50.0%
TGCAGTGGCCATTGGTGAAT+GGG - chr4_3:60123350-60123369 Msa0652410:intron 50.0%
TGGCCATTGGTGAATGGGAA+TGG - chr4_3:60123355-60123374 Msa0652410:intron 50.0%
GGCCATTGGTGAATGGGAAT+GGG - chr4_3:60123356-60123375 Msa0652410:intron 50.0%
GCAGGATGAAAATCGGAGTC+AGG - chr4_3:60124380-60124399 Msa0652410:intron 50.0%
TCAGGAAGAGAGAGAGTGGA+TGG - chr4_3:60124398-60124417 Msa0652410:intron 50.0%
CAGGAAGAGAGAGAGTGGAT+GGG - chr4_3:60124399-60124418 Msa0652410:intron 50.0%
TTCTGCTCTATGAAAGCCGG+TGG + chr4_3:60124540-60124559 None:intergenic 50.0%
AGAGGATGCTGTCCATCACT+TGG - chr4_3:60124718-60124737 Msa0652410:CDS 50.0%
! GGTGAAGTGTCATTGCCTGG+TGG - chr4_3:60122718-60122737 Msa0652410:intron 55.0%
GTCATTGCCTGGTGGGAAAG+CGG - chr4_3:60122726-60122745 Msa0652410:intron 55.0%
GAAAGCGGAGGAAGGGGATA+AGG - chr4_3:60122741-60122760 Msa0652410:intron 55.0%
!! GAAGTGCAGCTGAGGTACTG+AGG - chr4_3:60123250-60123269 Msa0652410:intron 55.0%
!! ACAGTTGGTGGCACGAGCAA+TGG + chr4_3:60123595-60123614 None:intergenic 55.0%
ACCATTTCAGAGTCGGTCCG+CGG - chr4_3:60123987-60124006 Msa0652410:CDS 55.0%
GGAGTCAGGAAGAGAGAGAG+TGG - chr4_3:60124394-60124413 Msa0652410:intron 55.0%
ATTGCCTGGTGGGAAAGCGG+AGG - chr4_3:60122729-60122748 Msa0652410:intron 60.0%
CTGGTGGGAAAGCGGAGGAA+GGG - chr4_3:60122734-60122753 Msa0652410:intron 60.0%
TGGTGGGAAAGCGGAGGAAG+GGG - chr4_3:60122735-60122754 Msa0652410:intron 60.0%
GGAAGGGGATAAGGACGACG+CGG - chr4_3:60122750-60122769 Msa0652410:intron 60.0%
! ACCGCGGACCGACTCTGAAA+TGG + chr4_3:60123991-60124010 None:intergenic 60.0%
CCTTCCTCCGCTTTCCCACC+AGG + chr4_3:60122736-60122755 None:intergenic 65.0%
CCTGGTGGGAAAGCGGAGGA+AGG - chr4_3:60122733-60122752 Msa0652410:intron 65.0%
CGCGGCCACTGCAAAACGAG+AGG - chr4_3:60122768-60122787 Msa0652410:intron 65.0%
TCAGAGTCGGTCCGCGGTCA+CGG - chr4_3:60123993-60124012 Msa0652410:CDS 65.0%
CAGAGTCGGTCCGCGGTCAC+GGG - chr4_3:60123994-60124013 Msa0652410:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 60121843 60124985 60121843 ID=Msa0652410;Name=Msa0652410
chr4_3 mRNA 60121843 60124985 60121843 ID=Msa0652410-mRNA-1;Parent=Msa0652410;Name=Msa0652410-mRNA-1;_AED=0.06;_eAED=0.06;_QI=187|1|1|1|0.66|1|4|374|282
chr4_3 exon 60121843 60122444 60121843 ID=Msa0652410-mRNA-1:exon:16301;Parent=Msa0652410-mRNA-1
chr4_3 exon 60122532 60122651 60122532 ID=Msa0652410-mRNA-1:exon:16300;Parent=Msa0652410-mRNA-1
chr4_3 exon 60123967 60124109 60123967 ID=Msa0652410-mRNA-1:exon:16299;Parent=Msa0652410-mRNA-1
chr4_3 exon 60124441 60124985 60124441 ID=Msa0652410-mRNA-1:exon:16298;Parent=Msa0652410-mRNA-1
chr4_3 five_prime_UTR 60124799 60124985 60124799 ID=Msa0652410-mRNA-1:five_prime_utr;Parent=Msa0652410-mRNA-1
chr4_3 CDS 60124441 60124798 60124441 ID=Msa0652410-mRNA-1:cds;Parent=Msa0652410-mRNA-1
chr4_3 CDS 60123967 60124109 60123967 ID=Msa0652410-mRNA-1:cds;Parent=Msa0652410-mRNA-1
chr4_3 CDS 60122532 60122651 60122532 ID=Msa0652410-mRNA-1:cds;Parent=Msa0652410-mRNA-1
chr4_3 CDS 60122217 60122444 60122217 ID=Msa0652410-mRNA-1:cds;Parent=Msa0652410-mRNA-1
chr4_3 three_prime_UTR 60121843 60122216 60121843 ID=Msa0652410-mRNA-1:three_prime_utr;Parent=Msa0652410-mRNA-1
Gene Sequence

>Msa0652410

ATGAATTCAATTCTAAGAACAATAACAACAACGTTAATACCAAGAACAGTATCTGTCACCAAAGTCATGGATTCTTCTTCCAGTGGTGGATCTCAGAGACTTCTTGCGTTGGCACAACATCTTCGTCTCTACAAGCCACCACCACCTTTTCCTGAAGATGTTTTGGAACAGAGCATTGAAGAGAGTGGTGGCAAAGTTGTTTCTCAATTGGGTTTTCCTGAATCAGCTACCATGATTCAAAATCCAGATAAATTTAGTCCTAAGAAAGCTGCTGTTTTGATTTGTTTATTTGAAGGTGATGATGGGGATCTAAGGGTTATTCTCACTAAACGATCTTCCAAGCTCTCTACTCACTCAGGTGAAGTGTCATTGCCTGGTGGGAAAGCGGAGGAAGGGGATAAGGACGACGCGGCCACTGCAAAACGAGAGGCAGAAGAGGAAATTGGGTTGGATCCAAAACTAGTCAATGTTGTTACTGTTCTTGAACCATTCTTGTCTAAGCACCTCCTAAGAGTAGTTCCTGTCATCGGCATACTTCATGATAAGAAAGCGTTCAAACCTGTTCTGAATCCTGCAGAAGTGGAATCAGTATTTGACGCACCTTTAGAAATGTTTCTCAAGGATGAAAATCGGAGTCAGGAAGAGAGAGAGTGGATGGGAGAGAAGTATTTGATACATTTTTTTGACTATATTGACATCGAGCAGAAAAAATATCTCATATGGGGATTAACTGCCGGAATTTTGATTAGGGCTGCATCAATTGTGTACAAACGGCCACCGGCTTTCATAGAGCAGAATCCCAAATTCAAAGTTCCACAGGTTGTAAGCAAGGATAGTTCAATGACTTGA
Protein sequence

>Msa0652410

MNSILRTITTTLIPRTVSVTKVMDSSSSGGSQRLLALAQHLRLYKPPPPFPEDVLEQSIEESGGKVVSQLGFPESATMIQNPDKFSPKKAAVLICLFEGDDGDLRVILTKRSSKLSTHSGEVSLPGGKAEEGDKDDAATAKREAEEEIGLDPKLVNVVTVLEPFLSKHLLRVVPVIGILHDKKAFKPVLNPAEVESVFDAPLEMFLKDENRSQEEREWMGEKYLIHFFDYIDIEQKKYLIWGLTAGILIRAASIVYKRPPAFIEQNPKFKVPQVVSKDSSMT*