AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0557050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0557050 MtrunA17_Chr4g0046091 95.455 286 9 2 1 282 1 286 0.0 550
Msa0557050 MtrunA17_Chr2g0283341 50.644 233 86 4 27 259 2 205 6.77e-76 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0557050 AT1G28960.3 70.775 284 77 4 3 281 10 292 4.79e-135 384
Msa0557050 AT1G28960.1 70.775 284 77 4 3 281 10 292 4.79e-135 384
Msa0557050 AT1G28960.5 70.775 284 77 4 3 281 10 292 4.79e-135 384
Msa0557050 AT1G28960.7 71.480 277 73 4 3 274 10 285 4.71e-133 378
Msa0557050 AT1G28960.6 71.480 277 73 4 3 274 10 285 4.71e-133 378
Msa0557050 AT2G33980.2 68.421 247 71 3 30 274 18 259 2.18e-115 332
Msa0557050 AT2G33980.4 68.421 247 71 3 30 274 18 259 2.18e-115 332
Msa0557050 AT2G33980.1 68.421 247 71 3 30 274 18 259 8.61e-115 332
Msa0557050 AT2G33980.3 68.421 247 71 3 30 274 18 259 1.36e-114 333
Msa0557050 AT5G45940.1 61.376 189 69 2 85 269 29 217 8.94e-79 238
Msa0557050 AT5G45940.2 63.281 128 44 1 85 209 29 156 5.06e-51 165
Msa0557050 AT5G45940.3 64.286 126 42 1 85 207 29 154 1.30e-50 164

Find 99 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTCTTCCTTTCTTTC+AGG 0.144264 4_1:+55347365 None:intergenic
AACCTGTGGAACTTTGAATT+TGG 0.148694 4_1:+55347507 None:intergenic
TGAATCATGGTAGCTGATTC+AGG 0.246118 4_1:+55349800 None:intergenic
GTAACAACATTGACTAGTTT+TGG 0.246703 4_1:+55349232 None:intergenic
TTCTTAGGACTAAATTTATC+TGG 0.253323 4_1:+55349773 None:intergenic
CGAGAGGCAGAAGAGGAAAT+TGG 0.270083 4_1:-55349262 Msa0557050:CDS
TCTTGAACCATTCTTGTCTA+AGG 0.274255 4_1:-55349206 Msa0557050:intron
CTCTGTTCCAAAACATCTTC+AGG 0.287071 4_1:+55349866 None:intergenic
TCCAGATCCCAAAATTAGTT+TGG 0.290730 4_1:+55347246 None:intergenic
ATTTCTGCTCACCTTGTGTT+TGG 0.302067 4_1:-55347321 Msa0557050:three_prime_UTR
TGGCAAAGTTGTTTCTCAAT+TGG 0.304222 4_1:-55349828 Msa0557050:CDS
ATCAAAACAGCAGCTTTCTT+AGG 0.317340 4_1:+55349758 None:intergenic
ACCTGTGGAACTTTGAATTT+GGG 0.326845 4_1:+55347508 None:intergenic
GCAAACTGCCATCACATCTA+TGG 0.329294 4_1:+55347128 None:intergenic
GGCAAAGTTGTTTCTCAATT+GGG 0.351294 4_1:-55349827 Msa0557050:CDS
CAATAACAATTTGATACTAT+AGG 0.355004 4_1:-55350090 Msa0557050:five_prime_UTR
AAGGTGATGATGGGGATCTA+AGG 0.355981 4_1:-55349724 Msa0557050:CDS
TTGGTGACAGATACTGTTCT+TGG 0.364656 4_1:+55349977 None:intergenic
ACTAGCAGTTTAATGTTTGA+AGG 0.371560 4_1:+55350150 None:intergenic
CGATGACAGGAACTACTCTT+AGG 0.397188 4_1:+55347887 None:intergenic
AATAACAATTTGATACTATA+GGG 0.401857 4_1:-55350089 Msa0557050:five_prime_UTR
TTGCAAAAGCAAATCTTAAC+CGG 0.409856 4_1:+55350115 None:intergenic
TGCAGCCCTAATCAAAATTC+CGG 0.409951 4_1:+55347573 None:intergenic
TCAGGAGGAGAGAGAGTGGA+TGG 0.414184 4_1:-55347671 Msa0557050:CDS
TAGAATCAATCAAATGGAAA+AGG 0.418865 4_1:-55347283 Msa0557050:three_prime_UTR
TGCTAGTTTAACTACTTGTC+CGG 0.424659 4_1:-55350134 Msa0557050:five_prime_UTR
CCTTCCTCCGCTTTCCCACC+AGG 0.426104 4_1:+55349313 None:intergenic
TACAATAATATATATTCGTC+AGG 0.427184 4_1:-55347404 Msa0557050:three_prime_UTR
AGAATCAATCAAATGGAAAA+GGG 0.434148 4_1:-55347282 Msa0557050:three_prime_UTR
TTTGAAGGTCTGCTCCTTGT+CGG 0.452475 4_1:+55350165 None:intergenic
GAAAGCGGAGGAAGGGGATA+AGG 0.452799 4_1:-55349305 Msa0557050:CDS
GATCGGTAGAATCAATCAAA+TGG 0.459510 4_1:-55347289 Msa0557050:three_prime_UTR
TGTTTATTTGAAGGTGATGA+TGG 0.461797 4_1:-55349734 Msa0557050:CDS
ACCTGAGTGAGTAGAGAGCT+TGG 0.464566 4_1:+55349679 None:intergenic
AATACTGATTCCACTTCTGC+AGG 0.466410 4_1:+55347823 None:intergenic
ACTTCTGCAGGATTCAGAAC+AGG 0.470641 4_1:+55347835 None:intergenic
TGGTGGGAAAGCGGAGGAAG+GGG 0.473889 4_1:-55349311 Msa0557050:CDS
GCTTTAAGCAGGATGAAAAT+CGG 0.478548 4_1:-55347696 Msa0557050:intron
CCTGGTGGGAAAGCGGAGGA+AGG 0.480967 4_1:-55349313 Msa0557050:CDS
TCCAAAACATCTTCAGGAAA+AGG 0.484289 4_1:+55349872 None:intergenic
GGAATAATAAAATGTTGTAA+AGG 0.484974 4_1:-55347226 Msa0557050:three_prime_UTR
ATTTATATCTCCTGAAAGAA+AGG 0.485574 4_1:-55347375 Msa0557050:three_prime_UTR
GAAATATGAATTCCAAGTGA+TGG 0.489702 4_1:+55347339 None:intergenic
GAGAGGCAGAAGAGGAAATT+GGG 0.494487 4_1:-55349261 Msa0557050:CDS
TGTTCTGAATCCTGCAGAAG+TGG 0.496762 4_1:-55347833 Msa0557050:CDS
CTGGTGGGAAAGCGGAGGAA+GGG 0.497359 4_1:-55349312 Msa0557050:CDS
GCAGGATGAAAATCGGAGTC+AGG 0.500021 4_1:-55347689 Msa0557050:intron
ACCTTGAGAAACATTTCTAA+AGG 0.503154 4_1:+55347793 None:intergenic
TCCCAAATTCAAAGTTCCAC+AGG 0.509189 4_1:-55347509 Msa0557050:CDS
CTGCATCAATTGTGTACAAA+CGG 0.513281 4_1:-55347555 Msa0557050:CDS
CTCATATGGGGATTGACTGC+CGG 0.519065 4_1:-55347592 Msa0557050:CDS
GGAAGAAGAATCCATGACTT+TGG 0.522178 4_1:+55349958 None:intergenic
ATCTCCTGAAAGAAAGGAAG+AGG 0.524063 4_1:-55347369 Msa0557050:three_prime_UTR
GGCAGAAGAGGAAATTGGGT+TGG 0.528513 4_1:-55349257 Msa0557050:CDS
ATGGATTCTTCTTCCAGTGG+TGG 0.530689 4_1:-55349950 Msa0557050:CDS
TCATGAAGTATGCCGATGAC+AGG 0.534330 4_1:+55347874 None:intergenic
AGAGGATGCTGTCCATCACT+TGG 0.536088 4_1:-55347351 Msa0557050:three_prime_UTR
CAGGAGGAGAGAGAGTGGAT+GGG 0.539082 4_1:-55347670 Msa0557050:CDS
GGAGTCAGGAGGAGAGAGAG+TGG 0.541128 4_1:-55347675 Msa0557050:CDS
GTTTATTTGAAGGTGATGAT+GGG 0.545292 4_1:-55349733 Msa0557050:CDS
GTGTTTGGATATCTAATGAT+CGG 0.547839 4_1:-55347306 Msa0557050:three_prime_UTR
ACCTTTAGAAATGTTTCTCA+AGG 0.548045 4_1:-55347794 Msa0557050:intron
GGATTCTGCTCTATGAAAGC+CGG 0.548477 4_1:+55347529 None:intergenic
AGGTGATGATGGGGATCTAA+GGG 0.549052 4_1:-55349723 Msa0557050:CDS
TGCAAAACGAGAGGCAGAAG+AGG 0.549211 4_1:-55349269 Msa0557050:CDS
ATCTCAGAGACTTCTTGCGT+TGG 0.549765 4_1:-55349927 Msa0557050:CDS
ATTTGATACTATAGGGTAGG+TGG 0.554437 4_1:-55350082 Msa0557050:five_prime_UTR
AGTTCCACAGGTTGTAAGCA+AGG 0.560370 4_1:-55347497 Msa0557050:CDS
CTATCCTTGCTTACAACCTG+TGG 0.562710 4_1:+55347493 None:intergenic
ATAGATACCTTAGACAAGAA+TGG 0.563801 4_1:+55349199 None:intergenic
AATTGTGTACAAACGGCCAC+CGG 0.564192 4_1:-55347548 Msa0557050:CDS
TCTTCAGGAAAAGGTGGTGG+TGG 0.564320 4_1:+55349881 None:intergenic
GTCATTGCCTGGTGGGAAAG+CGG 0.565959 4_1:-55349320 Msa0557050:CDS
TGAAATGATAAGCAATAAGA+TGG 0.579545 4_1:-55347188 Msa0557050:three_prime_UTR
AGTATCTGTCACCAAAGTCA+TGG 0.580073 4_1:-55349969 Msa0557050:CDS
CTAAGAGTAGTTCCTGTCAT+CGG 0.582497 4_1:-55347886 Msa0557050:CDS
GTAGGTGAAGTGTCATTGCC+TGG 0.586937 4_1:-55349331 Msa0557050:intron
AGGATAGTTCAATGACTTGA+CGG 0.587268 4_1:-55347477 Msa0557050:exon
CGCGGCCACTGCAAAACGAG+AGG 0.591310 4_1:-55349278 Msa0557050:CDS
ACATCTTCAGGAAAAGGTGG+TGG 0.591697 4_1:+55349878 None:intergenic
TCCAAGCTCTCTACTCACTC+AGG 0.594205 4_1:-55349680 Msa0557050:intron
ACAATTTGATACTATAGGGT+AGG 0.599534 4_1:-55350085 Msa0557050:five_prime_UTR
TTTGATACTATAGGGTAGGT+GGG 0.601183 4_1:-55350081 Msa0557050:five_prime_UTR
GTCATGGATTCTTCTTCCAG+TGG 0.604832 4_1:-55349953 Msa0557050:CDS
TGACAGGAACTACTCTTAGG+AGG 0.607814 4_1:+55347890 None:intergenic
AAGTCTCTGAGATCCACCAC+TGG 0.613439 4_1:+55349937 None:intergenic
GAATCAATCAAATGGAAAAG+GGG 0.620781 4_1:-55347281 Msa0557050:three_prime_UTR
TTTATTTGAAGGTGATGATG+GGG 0.628042 4_1:-55349732 Msa0557050:CDS
GGATGAAAATCGGAGTCAGG+AGG 0.629081 4_1:-55347686 Msa0557050:CDS
AAAACATCTTCAGGAAAAGG+TGG 0.635918 4_1:+55349875 None:intergenic
GAACAGAGCATTGAAGAGAG+TGG 0.639203 4_1:-55349851 Msa0557050:CDS
TTCTGCTCTATGAAAGCCGG+TGG 0.643205 4_1:+55347532 None:intergenic
GTGAAGTGTCATTGCCTGGT+GGG 0.658192 4_1:-55349327 Msa0557050:CDS
ATTGCCTGGTGGGAAAGCGG+AGG 0.658525 4_1:-55349317 Msa0557050:CDS
ACAAAGATTTCATTCCGACA+AGG 0.669608 4_1:-55350179 Msa0557050:five_prime_UTR
CAGAGCATTGAAGAGAGTGG+TGG 0.680698 4_1:-55349848 Msa0557050:CDS
GGTGAAGTGTCATTGCCTGG+TGG 0.686704 4_1:-55349328 Msa0557050:CDS
TCATTAGATATCCAAACACA+AGG 0.698944 4_1:+55347310 None:intergenic
GGAAGGGGATAAGGACGACG+CGG 0.736631 4_1:-55349296 Msa0557050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATCATTTTAAGAAAA+GGG + chr4_1:55348666-55348685 None:intergenic 10.0%
!!! TTATAAATCATTTTAAGAAA+AGG + chr4_1:55348667-55348686 None:intergenic 10.0%
!! AATAACAATTTGATACTATA+GGG - chr4_1:55347212-55347231 Msa0557050:three_prime_UTR 15.0%
!!! TTTTCTTTAATAGGAAAAAA+AGG + chr4_1:55348820-55348839 None:intergenic 15.0%
!! TGAAAAAAAGAAATATATTG+AGG + chr4_1:55349874-55349893 None:intergenic 15.0%
!!! AAAAAATTCCAAACTAATTT+TGG - chr4_1:55350047-55350066 Msa0557050:five_prime_UTR 15.0%
!!! AAAAATTCCAAACTAATTTT+GGG - chr4_1:55350048-55350067 Msa0557050:five_prime_UTR 15.0%
!! CAATAACAATTTGATACTAT+AGG - chr4_1:55347211-55347230 Msa0557050:three_prime_UTR 20.0%
!!! GTTTTGATTTGTTTATTTGA+AGG - chr4_1:55347558-55347577 Msa0557050:CDS 20.0%
!!! TACTTTAGTTATTGTGATTT+TGG - chr4_1:55347891-55347910 Msa0557050:CDS 20.0%
!!! TTTTGGTTATTTGAATTTAG+AGG - chr4_1:55347908-55347927 Msa0557050:intron 20.0%
!! TTTGGTTATTTGAATTTAGA+GGG - chr4_1:55347909-55347928 Msa0557050:intron 20.0%
!!! TTTCAGCAATTTTCTTTAAT+AGG + chr4_1:55348829-55348848 None:intergenic 20.0%
!!! GAAATTTATTTGTTGGATAA+TGG - chr4_1:55349131-55349150 Msa0557050:intron 20.0%
!! GGGTTATAAATTTGTATTAT+TGG - chr4_1:55349246-55349265 Msa0557050:CDS 20.0%
!! TACAATAATATATATTCGTC+AGG - chr4_1:55349897-55349916 Msa0557050:CDS 20.0%
!! GGAATAATAAAATGTTGTAA+AGG - chr4_1:55350075-55350094 Msa0557050:five_prime_UTR 20.0%
!! TCTTTGTCTTAGATTTTACA+TGG + chr4_1:55347109-55347128 None:intergenic 25.0%
!! TTTATCTGGATTTTGAATCA+TGG + chr4_1:55347517-55347536 None:intergenic 25.0%
! TTCTTAGGACTAAATTTATC+TGG + chr4_1:55347531-55347550 None:intergenic 25.0%
!!! TTTTTTCAGACAAATGTTAG+TGG - chr4_1:55347724-55347743 Msa0557050:intron 25.0%
! TAAAGATTCAAAATTCCAAG+TGG + chr4_1:55348179-55348198 None:intergenic 25.0%
!!! CTAGAAAATGTTGATTTTGT+TGG - chr4_1:55348247-55348266 Msa0557050:intron 25.0%
! TGTCTTTAAATCATGATTTG+TGG - chr4_1:55348272-55348291 Msa0557050:intron 25.0%
!! GTATTATATTTAGCTCATGA+AGG - chr4_1:55348294-55348313 Msa0557050:intron 25.0%
! ACTTCATTCTTGAATATCAT+CGG - chr4_1:55348436-55348455 Msa0557050:intron 25.0%
! AATTCCTGAAAACAATAAAC+AGG + chr4_1:55348703-55348722 None:intergenic 25.0%
! GATTGGTGAAATTTATTTGT+TGG - chr4_1:55349124-55349143 Msa0557050:intron 25.0%
! GATACAAATAAAACTAAGCA+TGG + chr4_1:55349535-55349554 None:intergenic 25.0%
! AGCAGAAAAAATATCTCATA+TGG - chr4_1:55349695-55349714 Msa0557050:CDS 25.0%
! GCAGAAAAAATATCTCATAT+GGG - chr4_1:55349696-55349715 Msa0557050:CDS 25.0%
! CAGAAAAAATATCTCATATG+GGG - chr4_1:55349697-55349716 Msa0557050:CDS 25.0%
! ATTTATATCTCCTGAAAGAA+AGG - chr4_1:55349926-55349945 Msa0557050:CDS 25.0%
! TAGAATCAATCAAATGGAAA+AGG - chr4_1:55350018-55350037 Msa0557050:CDS 25.0%
! AGAATCAATCAAATGGAAAA+GGG - chr4_1:55350019-55350038 Msa0557050:CDS 25.0%
! TGAAATGATAAGCAATAAGA+TGG - chr4_1:55350113-55350132 Msa0557050:five_prime_UTR 25.0%
!! TTTTTAATCCATAGATGTGA+TGG - chr4_1:55350165-55350184 Msa0557050:five_prime_UTR 25.0%
ACTAGCAGTTTAATGTTTGA+AGG + chr4_1:55347154-55347173 None:intergenic 30.0%
TTGCAAAAGCAAATCTTAAC+CGG + chr4_1:55347189-55347208 None:intergenic 30.0%
ACAATTTGATACTATAGGGT+AGG - chr4_1:55347216-55347235 Msa0557050:three_prime_UTR 30.0%
TGTTTATTTGAAGGTGATGA+TGG - chr4_1:55347567-55347586 Msa0557050:CDS 30.0%
GTTTATTTGAAGGTGATGAT+GGG - chr4_1:55347568-55347587 Msa0557050:CDS 30.0%
! TTTATTTGAAGGTGATGATG+GGG - chr4_1:55347569-55347588 Msa0557050:CDS 30.0%
AATTTAGAGGGAATGAATTG+AGG - chr4_1:55347921-55347940 Msa0557050:intron 30.0%
ATTTAGAGGGAATGAATTGA+GGG - chr4_1:55347922-55347941 Msa0557050:intron 30.0%
!!! GTAACAACATTGACTAGTTT+TGG + chr4_1:55348072-55348091 None:intergenic 30.0%
ATAGATACCTTAGACAAGAA+TGG + chr4_1:55348105-55348124 None:intergenic 30.0%
TTAATTGAGTATTCTCCACT+TGG - chr4_1:55348161-55348180 Msa0557050:intron 30.0%
AAATTTATGTCTGCGATTGA+TGG - chr4_1:55348201-55348220 Msa0557050:intron 30.0%
CCAAGACAACAATCATAATA+AGG + chr4_1:55348549-55348568 None:intergenic 30.0%
CCTTATTATGATTGTTGTCT+TGG - chr4_1:55348546-55348565 Msa0557050:intron 30.0%
! CTATCCTGTTTATTGTTTTC+AGG - chr4_1:55348696-55348715 Msa0557050:intron 30.0%
! CTAATCAGTTTGTTTACATC+AGG + chr4_1:55348730-55348749 None:intergenic 30.0%
CTGATGTAAACAAACTGATT+AGG - chr4_1:55348728-55348747 Msa0557050:intron 30.0%
AAAGCATAAGTTCGTTATTG+TGG + chr4_1:55348928-55348947 None:intergenic 30.0%
! TTATGCTTTGAACTAGATAG+AGG - chr4_1:55348939-55348958 Msa0557050:intron 30.0%
TAACAACACTCCATAGATTT+AGG - chr4_1:55349052-55349071 Msa0557050:intron 30.0%
!!! TGCATAAAGTTTTGAAACTG+TGG + chr4_1:55349157-55349176 None:intergenic 30.0%
GAGGAATAGACTAATAGAAA+AGG + chr4_1:55349312-55349331 None:intergenic 30.0%
GCTGTAAAAGAAACAATGAT+AGG + chr4_1:55349390-55349409 None:intergenic 30.0%
! ACCTTTAGAAATGTTTCTCA+AGG - chr4_1:55349507-55349526 Msa0557050:intron 30.0%
ACCTTGAGAAACATTTCTAA+AGG + chr4_1:55349511-55349530 None:intergenic 30.0%
GAAATATGAATTCCAAGTGA+TGG + chr4_1:55349965-55349984 None:intergenic 30.0%
TCATTAGATATCCAAACACA+AGG + chr4_1:55349994-55350013 None:intergenic 30.0%
GTGTTTGGATATCTAATGAT+CGG - chr4_1:55349995-55350014 Msa0557050:CDS 30.0%
GAATCAATCAAATGGAAAAG+GGG - chr4_1:55350020-55350039 Msa0557050:exon 30.0%
AAGATGGTGAAAAATGAACT+TGG - chr4_1:55350129-55350148 Msa0557050:five_prime_UTR 30.0%
ACAAAGATTTCATTCCGACA+AGG - chr4_1:55347122-55347141 Msa0557050:three_prime_UTR 35.0%
! TGCTAGTTTAACTACTTGTC+CGG - chr4_1:55347167-55347186 Msa0557050:three_prime_UTR 35.0%
ATTTGATACTATAGGGTAGG+TGG - chr4_1:55347219-55347238 Msa0557050:three_prime_UTR 35.0%
TTTGATACTATAGGGTAGGT+GGG - chr4_1:55347220-55347239 Msa0557050:three_prime_UTR 35.0%
AAAACATCTTCAGGAAAAGG+TGG + chr4_1:55347429-55347448 None:intergenic 35.0%
! ACCTTTTCCTGAAGATGTTT+TGG - chr4_1:55347428-55347447 Msa0557050:three_prime_UTR 35.0%
TCCAAAACATCTTCAGGAAA+AGG + chr4_1:55347432-55347451 None:intergenic 35.0%
!! TGGCAAAGTTGTTTCTCAAT+TGG - chr4_1:55347473-55347492 Msa0557050:exon 35.0%
!! GGCAAAGTTGTTTCTCAATT+GGG - chr4_1:55347474-55347493 Msa0557050:exon 35.0%
ATCAAAACAGCAGCTTTCTT+AGG + chr4_1:55347546-55347565 None:intergenic 35.0%
GCACATACACTTCTCATATT+AGG + chr4_1:55347796-55347815 None:intergenic 35.0%
AACCATTTGCCCAATAGTAT+AGG + chr4_1:55347853-55347872 None:intergenic 35.0%
ATACTATTGGGCAAATGGTT+TGG - chr4_1:55347853-55347872 Msa0557050:CDS 35.0%
TACTATTGGGCAAATGGTTT+GGG - chr4_1:55347854-55347873 Msa0557050:CDS 35.0%
! GGTGTGTGTGTTTTATATGT+AGG - chr4_1:55347952-55347971 Msa0557050:intron 35.0%
TCTTGAACCATTCTTGTCTA+AGG - chr4_1:55348095-55348114 Msa0557050:intron 35.0%
GTCTGTATAAAGTAATGCAG+TGG - chr4_1:55348594-55348613 Msa0557050:intron 35.0%
GGGTATGTATCTGATTCTTT+AGG + chr4_1:55348646-55348665 None:intergenic 35.0%
CTAAGAGATGTTGCATTCAA+TGG - chr4_1:55348784-55348803 Msa0557050:intron 35.0%
! TACATGACTTTTACAGTTGG+TGG + chr4_1:55348866-55348885 None:intergenic 35.0%
! GGATACATGACTTTTACAGT+TGG + chr4_1:55348869-55348888 None:intergenic 35.0%
AAAAGTCATGTATCCATTGC+AGG - chr4_1:55348874-55348893 Msa0557050:intron 35.0%
GTTATGCTGTCCTAAATCTA+TGG + chr4_1:55349065-55349084 None:intergenic 35.0%
ACGTTCTTATTAGCAACGAT+TGG - chr4_1:55349107-55349126 Msa0557050:intron 35.0%
AAGTATGACCATTTCAGAGT+CGG - chr4_1:55349212-55349231 Msa0557050:CDS 35.0%
ATGTTTACATTCCCTGTTGT+TGG - chr4_1:55349274-55349293 Msa0557050:CDS 35.0%
GTGTAAACTATCCAACAACA+GGG + chr4_1:55349288-55349307 None:intergenic 35.0%
TGTGTAAACTATCCAACAAC+AGG + chr4_1:55349289-55349308 None:intergenic 35.0%
! GCTTTAAGCAGGATGAAAAT+CGG - chr4_1:55349605-55349624 Msa0557050:intron 35.0%
CTGCATCAATTGTGTACAAA+CGG - chr4_1:55349746-55349765 Msa0557050:CDS 35.0%
ACCTGTGGAACTTTGAATTT+GGG + chr4_1:55349796-55349815 None:intergenic 35.0%
AACCTGTGGAACTTTGAATT+TGG + chr4_1:55349797-55349816 None:intergenic 35.0%
AGGATAGTTCAATGACTTGA+CGG - chr4_1:55349824-55349843 Msa0557050:CDS 35.0%
GATCGGTAGAATCAATCAAA+TGG - chr4_1:55350012-55350031 Msa0557050:CDS 35.0%
!!! TCCAAACTAATTTTGGGATC+TGG - chr4_1:55350054-55350073 Msa0557050:five_prime_UTR 35.0%
TCCAGATCCCAAAATTAGTT+TGG + chr4_1:55350058-55350077 None:intergenic 35.0%
! TTGGTGACAGATACTGTTCT+TGG + chr4_1:55347327-55347346 None:intergenic 40.0%
AGTATCTGTCACCAAAGTCA+TGG - chr4_1:55347332-55347351 Msa0557050:three_prime_UTR 40.0%
GGAAGAAGAATCCATGACTT+TGG + chr4_1:55347346-55347365 None:intergenic 40.0%
CTCTGTTCCAAAACATCTTC+AGG + chr4_1:55347438-55347457 None:intergenic 40.0%
! TGAATCATGGTAGCTGATTC+AGG + chr4_1:55347504-55347523 None:intergenic 40.0%
TTTGCCCAATAGTATAGGCT+GGG + chr4_1:55347848-55347867 None:intergenic 40.0%
ATTTGCCCAATAGTATAGGC+TGG + chr4_1:55347849-55347868 None:intergenic 40.0%
AGCCTATACTATTGGGCAAA+TGG - chr4_1:55347848-55347867 Msa0557050:CDS 40.0%
ACTATTGGGCAAATGGTTTG+GGG - chr4_1:55347855-55347874 Msa0557050:CDS 40.0%
! TGCGATTGATGGTTAGTTCA+TGG - chr4_1:55348212-55348231 Msa0557050:intron 40.0%
TACTTATTCCTCACACAGCA+TGG + chr4_1:55348325-55348344 None:intergenic 40.0%
TAAAGTAATGCAGTGGCCAT+TGG - chr4_1:55348601-55348620 Msa0557050:intron 40.0%
TCCATTGCAGGTCATTAAAC+TGG - chr4_1:55348886-55348905 Msa0557050:intron 40.0%
CCATTGCAGGTCATTAAACT+GGG - chr4_1:55348887-55348906 Msa0557050:intron 40.0%
CTTAAGTGCTCAGCATAAAC+AGG - chr4_1:55349012-55349031 Msa0557050:intron 40.0%
AAATTTATAACCCGTGACCG+CGG + chr4_1:55349239-55349258 None:intergenic 40.0%
AGTCTATTCCTCATCTGCTT+CGG - chr4_1:55349320-55349339 Msa0557050:CDS 40.0%
!! CGGATTGAAAGTGTTCAATG+AGG - chr4_1:55349340-55349359 Msa0557050:intron 40.0%
TGATAGGTGAGATGAAGTAC+TGG + chr4_1:55349374-55349393 None:intergenic 40.0%
CTAAGAGTAGTTCCTGTCAT+CGG - chr4_1:55349415-55349434 Msa0557050:intron 40.0%
!! AATACTGATTCCACTTCTGC+AGG + chr4_1:55349481-55349500 None:intergenic 40.0%
!!! TGACTGCCGGAATTTTGATT+AGG - chr4_1:55349722-55349741 Msa0557050:CDS 40.0%
!!! GACTGCCGGAATTTTGATTA+GGG - chr4_1:55349723-55349742 Msa0557050:CDS 40.0%
TGCAGCCCTAATCAAAATTC+CGG + chr4_1:55349731-55349750 None:intergenic 40.0%
TCCCAAATTCAAAGTTCCAC+AGG - chr4_1:55349792-55349811 Msa0557050:CDS 40.0%
ATCTCCTGAAAGAAAGGAAG+AGG - chr4_1:55349932-55349951 Msa0557050:CDS 40.0%
ATTTCTGCTCACCTTGTGTT+TGG - chr4_1:55349980-55349999 Msa0557050:CDS 40.0%
TTTGAAGGTCTGCTCCTTGT+CGG + chr4_1:55347139-55347158 None:intergenic 45.0%
GTCATGGATTCTTCTTCCAG+TGG - chr4_1:55347348-55347367 Msa0557050:three_prime_UTR 45.0%
ATGGATTCTTCTTCCAGTGG+TGG - chr4_1:55347351-55347370 Msa0557050:three_prime_UTR 45.0%
ATCTCAGAGACTTCTTGCGT+TGG - chr4_1:55347374-55347393 Msa0557050:three_prime_UTR 45.0%
ACATCTTCAGGAAAAGGTGG+TGG + chr4_1:55347426-55347445 None:intergenic 45.0%
!! GAACAGAGCATTGAAGAGAG+TGG - chr4_1:55347450-55347469 Msa0557050:three_prime_UTR 45.0%
!! AAGGTGATGATGGGGATCTA+AGG - chr4_1:55347577-55347596 Msa0557050:CDS 45.0%
!! AGGTGATGATGGGGATCTAA+GGG - chr4_1:55347578-55347597 Msa0557050:CDS 45.0%
AGATAACGTGTGTCTGACAC+AGG - chr4_1:55347764-55347783 Msa0557050:intron 45.0%
GATAACGTGTGTCTGACACA+GGG - chr4_1:55347765-55347784 Msa0557050:intron 45.0%
TGTGTCCCAGCCTATACTAT+TGG - chr4_1:55347840-55347859 Msa0557050:CDS 45.0%
GTGTCCCAGCCTATACTATT+GGG - chr4_1:55347841-55347860 Msa0557050:CDS 45.0%
! CTATTGGGCAAATGGTTTGG+GGG - chr4_1:55347856-55347875 Msa0557050:CDS 45.0%
AGGGAATGAATTGAGGGTTG+TGG - chr4_1:55347928-55347947 Msa0557050:intron 45.0%
GAATGAATTGAGGGTTGTGG+TGG - chr4_1:55347931-55347950 Msa0557050:intron 45.0%
GAGAGGCAGAAGAGGAAATT+GGG - chr4_1:55348040-55348059 Msa0557050:intron 45.0%
! TGCTCTGAGTCTGAGATGAT+TGG - chr4_1:55348352-55348371 Msa0557050:intron 45.0%
! GAGTCTGAGATGATTGGAGA+TGG - chr4_1:55348358-55348377 Msa0557050:intron 45.0%
! TCTGAGATGATTGGAGATGG+AGG - chr4_1:55348361-55348380 Msa0557050:intron 45.0%
CTGAGATGATTGGAGATGGA+GGG - chr4_1:55348362-55348381 Msa0557050:intron 45.0%
TCAGCTGCACTTCATGTATC+TGG + chr4_1:55348502-55348521 None:intergenic 45.0%
AGATACATGAAGTGCAGCTG+AGG - chr4_1:55348501-55348520 Msa0557050:intron 45.0%
ATGCCATTCCCATTCACCAA+TGG + chr4_1:55348620-55348639 None:intergenic 45.0%
TTCTTCACTGTCCACGTTCA+TGG - chr4_1:55348752-55348771 Msa0557050:intron 45.0%
AGTACGTACAACCATGAACG+TGG + chr4_1:55348766-55348785 None:intergenic 45.0%
CCCAGTTTAATGACCTGCAA+TGG + chr4_1:55348890-55348909 None:intergenic 45.0%
CTTTCAATCCGAAGCAGATG+AGG + chr4_1:55349331-55349350 None:intergenic 45.0%
TGACAGGAACTACTCTTAGG+AGG + chr4_1:55349414-55349433 None:intergenic 45.0%
CGATGACAGGAACTACTCTT+AGG + chr4_1:55349417-55349436 None:intergenic 45.0%
TCATGAAGTATGCCGATGAC+AGG + chr4_1:55349430-55349449 None:intergenic 45.0%
ACTTCTGCAGGATTCAGAAC+AGG + chr4_1:55349469-55349488 None:intergenic 45.0%
! TGTTCTGAATCCTGCAGAAG+TGG - chr4_1:55349468-55349487 Msa0557050:intron 45.0%
! CTGAAGCGATTGCTTTAAGC+AGG - chr4_1:55349594-55349613 Msa0557050:intron 45.0%
AATTGTGTACAAACGGCCAC+CGG - chr4_1:55349753-55349772 Msa0557050:CDS 45.0%
GGATTCTGCTCTATGAAAGC+CGG + chr4_1:55349775-55349794 None:intergenic 45.0%
AGTTCCACAGGTTGTAAGCA+AGG - chr4_1:55349804-55349823 Msa0557050:CDS 45.0%
CTATCCTTGCTTACAACCTG+TGG + chr4_1:55349811-55349830 None:intergenic 45.0%
GCATCCTCTTCCTTTCTTTC+AGG + chr4_1:55349939-55349958 None:intergenic 45.0%
GCAAACTGCCATCACATCTA+TGG + chr4_1:55350176-55350195 None:intergenic 45.0%
!! AAGTCTCTGAGATCCACCAC+TGG + chr4_1:55347367-55347386 None:intergenic 50.0%
! TCTTCAGGAAAAGGTGGTGG+TGG + chr4_1:55347423-55347442 None:intergenic 50.0%
!! CAGAGCATTGAAGAGAGTGG+TGG - chr4_1:55347453-55347472 Msa0557050:three_prime_UTR 50.0%
TCCAAGCTCTCTACTCACTC+AGG - chr4_1:55347621-55347640 Msa0557050:CDS 50.0%
ACCTGAGTGAGTAGAGAGCT+TGG + chr4_1:55347625-55347644 None:intergenic 50.0%
! GTAGGTGAAGTGTCATTGCC+TGG - chr4_1:55347970-55347989 Msa0557050:intron 50.0%
! GTGAAGTGTCATTGCCTGGT+GGG - chr4_1:55347974-55347993 Msa0557050:intron 50.0%
! TTCTGCCTCTCGTTTTGCAG+TGG + chr4_1:55348031-55348050 None:intergenic 50.0%
TGCAAAACGAGAGGCAGAAG+AGG - chr4_1:55348032-55348051 Msa0557050:intron 50.0%
CGAGAGGCAGAAGAGGAAAT+TGG - chr4_1:55348039-55348058 Msa0557050:intron 50.0%
GGCAGAAGAGGAAATTGGGT+TGG - chr4_1:55348044-55348063 Msa0557050:intron 50.0%
AGGAGATTCCATGCTGTGTG+AGG - chr4_1:55348314-55348333 Msa0557050:intron 50.0%
ATGCAGTGGCCATTGGTGAA+TGG - chr4_1:55348608-55348627 Msa0557050:intron 50.0%
TGCAGTGGCCATTGGTGAAT+GGG - chr4_1:55348609-55348628 Msa0557050:intron 50.0%
TGGCCATTGGTGAATGGGAA+TGG - chr4_1:55348614-55348633 Msa0557050:intron 50.0%
GCAGGATGAAAATCGGAGTC+AGG - chr4_1:55349612-55349631 Msa0557050:intron 50.0%
GGATGAAAATCGGAGTCAGG+AGG - chr4_1:55349615-55349634 Msa0557050:intron 50.0%
CTCATATGGGGATTGACTGC+CGG - chr4_1:55349709-55349728 Msa0557050:CDS 50.0%
TTCTGCTCTATGAAAGCCGG+TGG + chr4_1:55349772-55349791 None:intergenic 50.0%
AGAGGATGCTGTCCATCACT+TGG - chr4_1:55349950-55349969 Msa0557050:CDS 50.0%
! GGTGAAGTGTCATTGCCTGG+TGG - chr4_1:55347973-55347992 Msa0557050:intron 55.0%
GTCATTGCCTGGTGGGAAAG+CGG - chr4_1:55347981-55348000 Msa0557050:intron 55.0%
GAAAGCGGAGGAAGGGGATA+AGG - chr4_1:55347996-55348015 Msa0557050:intron 55.0%
!! GAAGTGCAGCTGAGGTACTG+AGG - chr4_1:55348509-55348528 Msa0557050:intron 55.0%
!! ACAGTTGGTGGCACGAGCAA+TGG + chr4_1:55348854-55348873 None:intergenic 55.0%
ACCATTTCAGAGTCGGTCCG+CGG - chr4_1:55349219-55349238 Msa0557050:CDS 55.0%
TCAGGAGGAGAGAGAGTGGA+TGG - chr4_1:55349630-55349649 Msa0557050:intron 55.0%
CAGGAGGAGAGAGAGTGGAT+GGG - chr4_1:55349631-55349650 Msa0557050:intron 55.0%
ATTGCCTGGTGGGAAAGCGG+AGG - chr4_1:55347984-55348003 Msa0557050:intron 60.0%
CTGGTGGGAAAGCGGAGGAA+GGG - chr4_1:55347989-55348008 Msa0557050:intron 60.0%
TGGTGGGAAAGCGGAGGAAG+GGG - chr4_1:55347990-55348009 Msa0557050:intron 60.0%
GGAAGGGGATAAGGACGACG+CGG - chr4_1:55348005-55348024 Msa0557050:intron 60.0%
! ACCGCGGACCGACTCTGAAA+TGG + chr4_1:55349223-55349242 None:intergenic 60.0%
GGAGTCAGGAGGAGAGAGAG+TGG - chr4_1:55349626-55349645 Msa0557050:intron 60.0%
CCTTCCTCCGCTTTCCCACC+AGG + chr4_1:55347991-55348010 None:intergenic 65.0%
CCTGGTGGGAAAGCGGAGGA+AGG - chr4_1:55347988-55348007 Msa0557050:intron 65.0%
CGCGGCCACTGCAAAACGAG+AGG - chr4_1:55348023-55348042 Msa0557050:intron 65.0%
TCAGAGTCGGTCCGCGGTCA+CGG - chr4_1:55349225-55349244 Msa0557050:CDS 65.0%
CAGAGTCGGTCCGCGGTCAC+GGG - chr4_1:55349226-55349245 Msa0557050:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 55347099 55350224 55347099 ID=Msa0557050;Name=Msa0557050
chr4_1 mRNA 55347099 55350224 55347099 ID=Msa0557050-mRNA-1;Parent=Msa0557050;Name=Msa0557050-mRNA-1;_AED=0.06;_eAED=0.06;_QI=186|1|1|1|0.66|1|4|381|282
chr4_1 exon 55347099 55347707 55347099 ID=Msa0557050-mRNA-1:exon:16511;Parent=Msa0557050-mRNA-1
chr4_1 exon 55347795 55347914 55347795 ID=Msa0557050-mRNA-1:exon:16510;Parent=Msa0557050-mRNA-1
chr4_1 exon 55349207 55349349 55349207 ID=Msa0557050-mRNA-1:exon:16509;Parent=Msa0557050-mRNA-1
chr4_1 exon 55349681 55350224 55349681 ID=Msa0557050-mRNA-1:exon:16508;Parent=Msa0557050-mRNA-1
chr4_1 five_prime_UTR 55350039 55350224 55350039 ID=Msa0557050-mRNA-1:five_prime_utr;Parent=Msa0557050-mRNA-1
chr4_1 CDS 55349681 55350038 55349681 ID=Msa0557050-mRNA-1:cds;Parent=Msa0557050-mRNA-1
chr4_1 CDS 55349207 55349349 55349207 ID=Msa0557050-mRNA-1:cds;Parent=Msa0557050-mRNA-1
chr4_1 CDS 55347795 55347914 55347795 ID=Msa0557050-mRNA-1:cds;Parent=Msa0557050-mRNA-1
chr4_1 CDS 55347480 55347707 55347480 ID=Msa0557050-mRNA-1:cds;Parent=Msa0557050-mRNA-1
chr4_1 three_prime_UTR 55347099 55347479 55347099 ID=Msa0557050-mRNA-1:three_prime_utr;Parent=Msa0557050-mRNA-1
Gene Sequence

>Msa0557050

ATGAATTCAATTCTAAGAACAATAACAACAACGTTAATACCAAGAACAGTATCTGTCACCAAAGTCATGGATTCTTCTTCCAGTGGTGGATCTCAGAGACTTCTTGCGTTGGCACAACATCTTCGTCTCTACAAGCCACCACCACCTTTTCCTGAAGATGTTTTGGAACAGAGCATTGAAGAGAGTGGTGGCAAAGTTGTTTCTCAATTGGGTTTTCCTGAATCAGCTACCATGATTCAAAATCCAGATAAATTTAGTCCTAAGAAAGCTGCTGTTTTGATTTGTTTATTTGAAGGTGATGATGGGGATCTAAGGGTTATTCTCACTAAACGATCTTCCAAGCTCTCTACTCACTCAGGTGAAGTGTCATTGCCTGGTGGGAAAGCGGAGGAAGGGGATAAGGACGACGCGGCCACTGCAAAACGAGAGGCAGAAGAGGAAATTGGGTTGGATCCAAAACTAGTCAATGTTGTTACTGTTCTTGAACCATTCTTGTCTAAGCACCTCCTAAGAGTAGTTCCTGTCATCGGCATACTTCATGATAAGAAAGCGTTCAAACCTGTTCTGAATCCTGCAGAAGTGGAATCAGTATTTGACGCACCTTTAGAAATGTTTCTCAAGGATGAAAATCGGAGTCAGGAGGAGAGAGAGTGGATGGGAGAGAAGTATTTGATACATTTTTTTGACTATATTGACATCGAGCAGAAAAAATATCTCATATGGGGATTGACTGCCGGAATTTTGATTAGGGCTGCATCAATTGTGTACAAACGGCCACCGGCTTTCATAGAGCAGAATCCCAAATTCAAAGTTCCACAGGTTGTAAGCAAGGATAGTTCAATGACTTGA
Protein sequence

>Msa0557050

MNSILRTITTTLIPRTVSVTKVMDSSSSGGSQRLLALAQHLRLYKPPPPFPEDVLEQSIEESGGKVVSQLGFPESATMIQNPDKFSPKKAAVLICLFEGDDGDLRVILTKRSSKLSTHSGEVSLPGGKAEEGDKDDAATAKREAEEEIGLDPKLVNVVTVLEPFLSKHLLRVVPVIGILHDKKAFKPVLNPAEVESVFDAPLEMFLKDENRSQEEREWMGEKYLIHFFDYIDIEQKKYLIWGLTAGILIRAASIVYKRPPAFIEQNPKFKVPQVVSKDSSMT*