AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0663000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0663000 MtrunA17_Chr4g0021641 97.744 266 6 0 1 266 1 266 0.0 545
Msa0663000 MtrunA17_Chr2g0324221 80.263 228 45 0 37 264 31 258 7.55e-144 404
Msa0663000 MtrunA17_Chr2g0324201 78.571 238 50 1 28 264 19 256 3.76e-143 402
Msa0663000 MtrunA17_Chr7g0231221 47.321 224 114 4 45 264 52 275 3.96e-72 222
Msa0663000 MtrunA17_Chr4g0012801 42.045 264 142 6 7 266 10 266 7.36e-69 213
Msa0663000 MtrunA17_Chr7g0231211 45.495 222 115 4 46 264 52 270 6.20e-65 204
Msa0663000 MtrunA17_Chr7g0218741 32.576 264 152 8 16 264 1 253 1.23e-33 122
Msa0663000 MtrunA17_Chr4g0057091 34.167 240 130 9 41 265 36 262 2.70e-32 119
Msa0663000 MtrunA17_Chr5g0398271 34.167 240 130 10 41 265 37 263 1.73e-31 117
Msa0663000 MtrunA17_Chr8g0360041 31.599 269 146 8 9 264 11 254 5.22e-31 115
Msa0663000 MtrunA17_Chr7g0218751 31.559 263 154 9 16 264 1 251 3.35e-30 113
Msa0663000 MtrunA17_Chr3g0092071 33.333 231 130 9 41 259 25 243 4.81e-28 107
Msa0663000 MtrunA17_Chr1g0193631 31.799 239 137 9 41 264 30 257 4.99e-28 108
Msa0663000 MtrunA17_Chr4g0021561 32.353 238 135 10 38 261 29 254 7.67e-28 107
Msa0663000 MtrunA17_Chr7g0217661 32.800 250 149 10 16 261 4 238 2.48e-27 105
Msa0663000 MtrunA17_Chr4g0055061 32.287 223 135 9 46 260 34 248 2.15e-26 103
Msa0663000 MtrunA17_Chr4g0038741 28.745 247 157 6 22 264 12 243 3.65e-26 103
Msa0663000 MtrunA17_Chr7g0271001 31.381 239 138 9 41 264 27 254 4.28e-26 102
Msa0663000 MtrunA17_Chr5g0417701 32.468 231 132 9 41 259 25 243 8.17e-26 102
Msa0663000 MtrunA17_Chr1g0157061 32.159 227 127 9 53 264 27 241 8.50e-26 101
Msa0663000 MtrunA17_Chr5g0399811 29.646 226 144 7 46 264 34 251 2.49e-25 100
Msa0663000 MtrunA17_Chr3g0128801 32.189 233 134 9 41 261 31 251 2.54e-25 100
Msa0663000 MtrunA17_Chr3g0128791 32.035 231 133 9 41 259 31 249 1.09e-24 99.0
Msa0663000 MtrunA17_Chr5g0432611 31.739 230 133 9 41 258 29 246 6.69e-24 97.1
Msa0663000 MtrunA17_Chr4g0024971 30.811 185 81 5 95 237 4 183 9.70e-24 95.1
Msa0663000 MtrunA17_Chr4g0042521 31.169 231 135 9 41 259 25 243 1.03e-23 96.3
Msa0663000 MtrunA17_Chr4g0055601 29.060 234 144 9 40 262 27 249 1.45e-23 95.9
Msa0663000 MtrunA17_Chr7g0218761 31.797 217 124 8 60 264 8 212 3.04e-23 94.4
Msa0663000 MtrunA17_Chr5g0435311 31.489 235 135 10 41 261 82 304 4.48e-23 95.9
Msa0663000 MtrunA17_Chr4g0055561 26.740 273 168 10 1 264 1 250 6.72e-23 94.4
Msa0663000 MtrunA17_Chr4g0069711 29.675 246 144 10 31 261 51 282 1.12e-22 94.4
Msa0663000 MtrunA17_Chr3g0107291 28.897 263 162 10 9 258 5 255 1.85e-22 93.2
Msa0663000 MtrunA17_Chr5g0433841 29.362 235 141 8 40 261 40 262 6.98e-22 91.7
Msa0663000 MtrunA17_Chr7g0238681 31.064 235 136 11 41 261 32 254 3.76e-21 89.7
Msa0663000 MtrunA17_Chr3g0107311 29.565 230 138 9 41 258 32 249 8.72e-21 88.6
Msa0663000 MtrunA17_Chr2g0328261 30.213 235 138 11 41 261 34 256 1.15e-20 88.6
Msa0663000 MtrunA17_Chr6g0453341 30.131 229 144 7 41 265 38 254 3.80e-20 87.0
Msa0663000 MtrunA17_Chr8g0360721 30.415 217 127 7 60 264 17 221 2.36e-19 84.0
Msa0663000 MtrunA17_Chr4g0031621 32.990 194 104 10 40 219 31 212 1.35e-17 80.1
Msa0663000 MtrunA17_Chr2g0324191 31.646 158 82 5 16 159 7 152 1.12e-15 72.4
Msa0663000 MtrunA17_Chr4g0024981 43.011 93 46 2 5 97 14 99 2.56e-15 70.5
Msa0663000 MtrunA17_Chr3g0109801 28.395 243 141 10 40 261 52 282 3.29e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0663000 AT4G28250.1 73.200 250 66 1 16 264 11 260 1.56e-138 390
Msa0663000 AT4G28250.2 71.600 250 64 2 16 264 11 254 1.39e-132 375
Msa0663000 AT2G20750.1 62.687 268 95 3 1 264 1 267 4.12e-123 352
Msa0663000 AT4G28250.4 71.658 187 47 1 16 202 11 191 2.75e-95 279
Msa0663000 AT4G28250.3 71.658 187 47 1 16 202 11 191 3.12e-95 279
Msa0663000 AT2G20750.2 60.000 205 78 2 1 202 1 204 4.11e-85 253
Msa0663000 AT1G65680.1 47.414 232 119 3 36 264 42 273 2.00e-77 236
Msa0663000 AT3G60570.1 41.797 256 143 5 11 264 6 257 2.78e-70 217
Msa0663000 AT2G45110.1 43.426 251 134 6 20 264 11 259 2.79e-65 204
Msa0663000 AT3G60570.2 43.541 209 114 4 58 264 3 209 1.05e-62 196
Msa0663000 AT1G65681.1 42.791 215 116 5 54 264 11 222 2.47e-54 175
Msa0663000 AT1G12560.1 33.193 238 132 8 41 264 37 261 1.15e-30 115
Msa0663000 AT2G40610.1 33.597 253 145 9 18 258 7 248 2.49e-30 114
Msa0663000 AT4G17030.1 32.883 222 138 6 46 262 32 247 4.24e-30 113
Msa0663000 AT1G62980.1 32.308 260 148 9 20 264 10 256 8.23e-30 113
Msa0663000 AT5G39270.1 32.773 238 138 7 37 264 54 279 1.04e-29 113
Msa0663000 AT5G39290.1 33.193 238 137 8 37 264 54 279 1.73e-29 112
Msa0663000 AT5G05290.1 32.468 231 131 9 41 258 32 250 5.08e-28 108
Msa0663000 AT2G03090.1 32.636 239 137 9 35 261 24 250 6.33e-28 108
Msa0663000 AT3G15370.1 34.118 255 141 11 18 261 9 247 1.05e-27 107
Msa0663000 AT5G56320.1 31.760 233 135 8 41 261 29 249 1.21e-26 104
Msa0663000 AT5G56320.2 31.760 233 135 8 41 261 29 249 1.21e-26 104
Msa0663000 AT5G39280.1 31.799 239 139 9 37 264 50 275 2.66e-26 104
Msa0663000 AT5G56320.3 31.760 233 135 8 41 261 29 249 3.27e-26 104
Msa0663000 AT5G39300.1 31.381 239 140 9 37 264 51 276 5.32e-26 103
Msa0663000 AT5G02260.1 27.907 258 160 9 18 261 9 254 3.75e-25 100
Msa0663000 AT5G39260.1 30.147 272 167 11 3 264 4 262 5.55e-25 100
Msa0663000 AT4G38400.1 31.579 190 120 5 77 260 59 244 8.63e-25 100
Msa0663000 AT3G15370.2 35.981 214 115 10 59 261 75 277 5.28e-24 98.2
Msa0663000 AT4G01630.1 28.226 248 152 9 32 264 19 255 6.67e-24 97.4
Msa0663000 AT3G29030.1 32.589 224 134 9 41 259 38 249 9.38e-24 97.1
Msa0663000 AT3G03220.1 29.787 235 140 6 40 261 39 261 1.44e-22 94.0
Msa0663000 AT1G69530.2 31.169 231 135 9 41 259 27 245 1.54e-22 93.6
Msa0663000 AT1G69530.1 31.169 231 135 9 41 259 27 245 1.54e-22 93.6
Msa0663000 AT2G37640.1 29.787 235 139 9 41 261 36 258 1.98e-22 93.6
Msa0663000 AT1G69530.3 31.169 231 135 9 41 259 27 245 2.03e-22 94.0
Msa0663000 AT3G45970.2 30.144 209 134 7 59 260 14 217 2.20e-22 92.8
Msa0663000 AT2G39700.1 30.189 265 154 12 16 261 1 253 2.79e-22 93.2
Msa0663000 AT3G55500.1 30.638 235 137 11 41 261 34 256 4.36e-22 92.8
Msa0663000 AT1G26770.1 30.870 230 135 9 41 258 26 243 2.81e-21 90.1
Msa0663000 AT1G26770.2 30.870 230 135 9 41 258 36 253 3.23e-21 90.1
Msa0663000 AT3G45960.1 30.220 182 117 5 77 252 10 187 5.54e-21 88.6
Msa0663000 AT1G20190.1 30.508 236 137 9 44 264 29 252 8.37e-21 89.0
Msa0663000 AT3G45960.2 30.220 182 117 5 77 252 58 235 1.15e-20 89.0
Msa0663000 AT3G45970.1 30.366 191 122 6 77 260 58 244 6.62e-20 86.7
Msa0663000 AT1G69530.5 33.508 191 103 9 41 219 27 205 2.94e-19 84.7
Msa0663000 AT1G69530.4 33.508 191 103 9 41 219 27 205 2.98e-19 84.0
Msa0663000 AT4G38210.1 27.530 247 151 9 31 261 18 252 1.37e-18 83.2
Msa0663000 AT2G37640.2 29.493 217 127 9 59 261 29 233 2.94e-18 81.6
Msa0663000 AT2G28950.1 30.213 235 138 11 41 261 31 253 1.04e-17 80.5

Find 158 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTATTGGTGGACCTTTA+AGG 0.121240 4_4:-1062323 Msa0663000:CDS
ATGAAGGTTCCACACCATTT+TGG 0.241028 4_4:-1064035 Msa0663000:CDS
GACCTCAGCGGTGCTGCTTT+TGG 0.243260 4_4:-1065333 Msa0663000:CDS
ACAGAGAATGGTCCCCTTAA+AGG 0.251845 4_4:+1062309 None:intergenic
GCCTCAGATAGGAGCTAAAT+TGG 0.257477 4_4:+1066019 None:intergenic
CCGGTACTGCCGTCTCCTTC+GGG 0.275055 4_4:+1065874 None:intergenic
CAGCACCGCTGAGGTCAAAA+TGG 0.279998 4_4:+1065340 None:intergenic
GTGGTGTCAGTTGGGCAGCC+TGG 0.282084 4_4:+1065363 None:intergenic
TTCAACTCTGCAAGGAATTA+AGG 0.284393 4_4:+1061816 None:intergenic
ACACGCTCTCTCTCCAGTTT+TGG 0.297811 4_4:+1061849 None:intergenic
ACCGGTACTGCCGTCTCCTT+CGG 0.306865 4_4:+1065873 None:intergenic
GGTGCAAATTGGTGCATTAT+TGG 0.308201 4_4:-1062336 Msa0663000:CDS
CTGTTGTGGCGTTTGGGTTT+AGG 0.311141 4_4:+1066147 None:intergenic
AATTGCACAAGGGCATTAAA+TGG 0.317788 4_4:-1061963 Msa0663000:three_prime_UTR
CATTATTGGTGGACCTTTAA+GGG 0.321218 4_4:-1062322 Msa0663000:CDS
GAAGATGCAGAGGGAGATAT+TGG 0.322570 4_4:-1063991 Msa0663000:CDS
ATTCTGTCTGTTGTGGCGTT+TGG 0.323637 4_4:+1066140 None:intergenic
CTAGTTAATTAATTAAAGTT+TGG 0.327213 4_4:-1062101 Msa0663000:three_prime_UTR
TGGTGTCAGTTGGGCAGCCT+GGG 0.333933 4_4:+1065364 None:intergenic
ACCAAGGCTCCTTCCTATAG+TGG 0.335513 4_4:-1062146 Msa0663000:three_prime_UTR
GAGGGCGAGGGTAGGGGCAG+TGG 0.337642 4_4:-1065499 Msa0663000:CDS
CATGTAGCCCTCTCCAAAAC+TGG 0.345429 4_4:-1061862 Msa0663000:three_prime_UTR
CATGAAGCTCGCCCTGCTTC+CGG 0.346597 4_4:-1065977 Msa0663000:CDS
GAGATGTTGTTCCTAGCAAT+TGG 0.359807 4_4:-1062248 Msa0663000:CDS
CCGCGGTGGCAGCCGGAAGC+AGG 0.362758 4_4:+1065965 None:intergenic
TTCTGTCTGTTGTGGCGTTT+GGG 0.364670 4_4:+1066141 None:intergenic
ACTAGAAGTGATAACCAAAA+TGG 0.367256 4_4:+1064021 None:intergenic
CGGAAGCAGGGCGAGCTTCA+TGG 0.370859 4_4:+1065978 None:intergenic
TAAAACACAACCTTTGCAAT+TGG 0.373153 4_4:-1062183 Msa0663000:three_prime_UTR
GAACAACATCTCTAGCTGAT+AGG 0.376276 4_4:+1062259 None:intergenic
CACAAAACCAACGGACAAAA+AGG 0.380976 4_4:-1066234 Msa0663000:five_prime_UTR
TAACACACCATAATAAAATT+AGG 0.385942 4_4:+1061902 None:intergenic
TTATTATGGTGTGTTATTGT+TGG 0.395137 4_4:-1061895 Msa0663000:three_prime_UTR
GCCCCTACCCTCGCCCTCAA+TGG 0.395548 4_4:+1065504 None:intergenic
ATCATCTGTGGGGTGCAAAT+TGG 0.407156 4_4:-1062347 Msa0663000:CDS
CCGTTAGAAGAGAGGGTTGT+TGG 0.408166 4_4:+1066175 None:intergenic
AATGCCCTTGTGCAATTCAA+TGG 0.409598 4_4:+1061969 None:intergenic
CATTGTACACTACTGTTGTT+CGG 0.415383 4_4:+1066047 None:intergenic
AGCCATTGAGGGCGAGGGTA+GGG 0.417453 4_4:-1065506 Msa0663000:CDS
CGAGAAGTGTAAGTGGCCTT+TGG 0.426749 4_4:+1062222 None:intergenic
AGTATTCTTTAAAGTCCAAC+TGG 0.428064 4_4:+1061780 None:intergenic
CTTCCGGCTGCCACCGCGGT+TGG 0.428201 4_4:-1065961 Msa0663000:CDS
ATTGCATTTCATGTTAATGA+AGG 0.430261 4_4:-1064051 Msa0663000:CDS
GCCATTGAGGGCGAGGGTAG+GGG 0.437817 4_4:-1065505 Msa0663000:CDS
TAGATATATATAGCAAACTA+AGG 0.442150 4_4:+1062028 None:intergenic
CAAACTTTAATTAATTAACT+AGG 0.442906 4_4:+1062102 None:intergenic
ATTTAAAGACATTCAAAGGT+TGG 0.444255 4_4:+1061751 None:intergenic
TAATTAATTAAAGTTTGGTT+AGG 0.454999 4_4:-1062096 Msa0663000:three_prime_UTR
GTGAATTAGGTGGAGCTTGT+GGG 0.465048 4_4:-1065551 Msa0663000:intron
AGATGTTGTTCCTAGCAATT+GGG 0.466992 4_4:-1062247 Msa0663000:CDS
AAACTAAGGGAAAGAGAGTT+GGG 0.468055 4_4:+1062042 None:intergenic
TTATGGTGTGTTATTGTTGG+TGG 0.468886 4_4:-1061892 Msa0663000:three_prime_UTR
CGCGGTGGCAGCCGGAAGCA+GGG 0.473685 4_4:+1065966 None:intergenic
ACCATACCAGGTAGCGGTAC+CGG 0.476252 4_4:+1065900 None:intergenic
CCCGAAGGAGACGGCAGTAC+CGG 0.476399 4_4:-1065874 Msa0663000:intron
TTAGGTGGAGCTTGTGGGTA+TGG 0.479923 4_4:-1065546 Msa0663000:intron
AACTGGAGAGAGAGCGTGTA+GGG 0.484316 4_4:-1061845 Msa0663000:three_prime_UTR
TAGACAATAGCATATGTTCA+AGG 0.484691 4_4:-1065419 Msa0663000:CDS
AGAACTCCGTGTAAATATCC+AGG 0.486166 4_4:-1064081 Msa0663000:CDS
TGTCCAACCGCGGTGGCAGC+CGG 0.486551 4_4:+1065958 None:intergenic
AACAACATCTCTAGCTGATA+GGG 0.486552 4_4:+1062260 None:intergenic
CAAAATGGGTGGTGTCAGTT+GGG 0.487132 4_4:+1065355 None:intergenic
CCCGGTACCGCTACCTGGTA+TGG 0.488417 4_4:-1065901 Msa0663000:CDS
ACTGTTGTTCGGAGCTGAAA+TGG 0.489208 4_4:+1066058 None:intergenic
TAAAATTTAAAGACATTCAA+AGG 0.489377 4_4:+1061747 None:intergenic
GGTTATTAATAACCCACTAT+AGG 0.493961 4_4:+1062133 None:intergenic
GGATTCAGTGTGGGTCCTAC+CGG 0.497262 4_4:+1066283 None:intergenic
TCAAAATGGGTGGTGTCAGT+TGG 0.498024 4_4:+1065354 None:intergenic
TCCTTCGGGGTCACCATACC+AGG 0.500366 4_4:+1065888 None:intergenic
CAAACTAAGGGAAAGAGAGT+TGG 0.504470 4_4:+1062041 None:intergenic
AAGCCATTGAGGGCGAGGGT+AGG 0.505967 4_4:-1065507 Msa0663000:CDS
TCCTAGCAATTGGGTTCCAA+AGG 0.508249 4_4:-1062238 Msa0663000:CDS
TCCCCTTTCATAAACAGCAC+TGG 0.510386 4_4:+1065474 None:intergenic
AAGAGAAACAAGAAAAGAGA+AGG 0.511058 4_4:+1061939 None:intergenic
GAAGCAGAAAAGCCTCAGAT+AGG 0.511638 4_4:+1066008 None:intergenic
GACCAGTGCTGTTTATGAAA+GGG 0.512227 4_4:-1065476 Msa0663000:CDS
AGGGCGAGGGTAGGGGCAGT+GGG 0.514219 4_4:-1065498 Msa0663000:CDS
TTAATTAACTAGGTAAAATG+TGG 0.518656 4_4:+1062112 None:intergenic
GAGCTCTGCCGACAGTTTCG+TGG 0.519615 4_4:+1066091 None:intergenic
GAGCTGAGTTTAACAGAGAA+TGG 0.520854 4_4:+1062297 None:intergenic
ATCATACTCTTGACTTACAT+TGG 0.522403 4_4:-1065927 Msa0663000:CDS
GGTATCCCGGTACCGCTACC+TGG 0.525360 4_4:-1065906 Msa0663000:CDS
TGGTGGACGTGAAGCCATTG+AGG 0.525762 4_4:-1065518 Msa0663000:CDS
AGCTTGTGGGTATGGAACAT+TGG 0.530049 4_4:-1065538 Msa0663000:CDS
AGCACCGCTGAGGTCAAAAT+GGG 0.530672 4_4:+1065341 None:intergenic
CCATACCAGGTAGCGGTACC+GGG 0.535084 4_4:+1065901 None:intergenic
TGTGAATTAGGTGGAGCTTG+TGG 0.538467 4_4:-1065552 Msa0663000:intron
GGTTGAACCGTTAGAAGAGA+GGG 0.538531 4_4:+1066168 None:intergenic
TATGGCTATCTCCGGTGAGA+AGG 0.540158 4_4:-1065307 Msa0663000:CDS
ACGTGAAGCCATTGAGGGCG+AGG 0.541645 4_4:-1065512 Msa0663000:CDS
CTATAGGAAGGAGCCTTGGT+TGG 0.543126 4_4:+1062149 None:intergenic
GGACCAGTGCTGTTTATGAA+AGG 0.546468 4_4:-1065477 Msa0663000:CDS
TTTGGACATATGGCTATCTC+CGG 0.546777 4_4:-1065315 Msa0663000:CDS
CTTGACTTACATTGGTATCC+CGG 0.547303 4_4:-1065919 Msa0663000:CDS
ATGGCTATCTCCGGTGAGAA+GGG 0.550564 4_4:-1065306 Msa0663000:CDS
GGTGGACGTGAAGCCATTGA+GGG 0.556631 4_4:-1065517 Msa0663000:CDS
GGCAGAGAGAGCAAGAGAGA+GGG 0.559341 4_4:+1066115 None:intergenic
GCAAATTGGTGCATTATTGG+TGG 0.562847 4_4:-1062333 Msa0663000:CDS
TGCAGAATGGTTCTAGTGAG+TGG 0.562862 4_4:-1062380 Msa0663000:intron
CGGCAGAGAGAGCAAGAGAG+AGG 0.564499 4_4:+1066114 None:intergenic
TCTAGCTGATAGGGTTCTAC+CGG 0.567735 4_4:+1062269 None:intergenic
GCCTTTGGAACCCAATTGCT+AGG 0.569430 4_4:+1062237 None:intergenic
TATGGTGACCCCGAAGGAGA+CGG 0.569807 4_4:-1065883 Msa0663000:CDS
CCCACTATAGGAAGGAGCCT+TGG 0.572754 4_4:+1062145 None:intergenic
TCTTAGTTGACCCTTCTCAC+CGG 0.574534 4_4:+1065296 None:intergenic
ACCAGTGCTGTTTATGAAAG+GGG 0.580566 4_4:-1065475 Msa0663000:CDS
CCTGCTTCCGGCTGCCACCG+CGG 0.582293 4_4:-1065965 Msa0663000:CDS
TTACTGAAGTGTGCCACTGA+GGG 0.583048 4_4:+1066262 None:intergenic
ATTAATAACCCACTATAGGA+AGG 0.584565 4_4:+1062137 None:intergenic
CCAAGGCTCCTTCCTATAGT+GGG 0.586391 4_4:-1062145 Msa0663000:three_prime_UTR
GCCACTGAGGGATTCAGTGT+GGG 0.587532 4_4:+1066274 None:intergenic
GAGGGAGATTCTGTCTGTTG+TGG 0.588125 4_4:+1066133 None:intergenic
GGCTACAAATGAATCATCTG+TGG 0.590028 4_4:-1062359 Msa0663000:CDS
CCAACAACCCTCTCTTCTAA+CGG 0.592065 4_4:-1066175 Msa0663000:five_prime_UTR
TTTACCTTCTGTAAATGACA+GGG 0.593015 4_4:+1065259 None:intergenic
ATTATTGGTGGACCTTTAAG+GGG 0.593123 4_4:-1062321 Msa0663000:CDS
ATAATCACTGACGAGTGCCC+AGG 0.594245 4_4:-1065381 Msa0663000:CDS
AGGTTGAACCGTTAGAAGAG+AGG 0.595256 4_4:+1066167 None:intergenic
TCCAATTTAGCTCCTATCTG+AGG 0.598649 4_4:-1066020 Msa0663000:CDS
AGCTGATAGGGTTCTACCGG+TGG 0.602382 4_4:+1062272 None:intergenic
AGTTGAATTTGAAGATGCAG+AGG 0.603522 4_4:-1064001 Msa0663000:CDS
GTGCTGTTTATGAAAGGGGA+AGG 0.604394 4_4:-1065471 Msa0663000:CDS
TACACACGTTCAACTCTGCA+AGG 0.604411 4_4:+1061808 None:intergenic
CTCTGCCGCCACGAAACTGT+CGG 0.605791 4_4:-1066099 Msa0663000:five_prime_UTR
ATTTAGGCGAGAAGTGTAAG+TGG 0.607247 4_4:+1062215 None:intergenic
GTAAATAAACACAAAACCAA+CGG 0.607324 4_4:-1066243 Msa0663000:five_prime_UTR
GTTGAATTTGAAGATGCAGA+GGG 0.613537 4_4:-1064000 Msa0663000:CDS
AAATCCCTGTCATTTACAGA+AGG 0.616492 4_4:-1065263 Msa0663000:intron
AGATATATATAGCAAACTAA+GGG 0.618851 4_4:+1062029 None:intergenic
CGTGAAGCCATTGAGGGCGA+GGG 0.618982 4_4:-1065511 Msa0663000:CDS
AAGTGATAACCAAAATGGTG+TGG 0.620271 4_4:+1064026 None:intergenic
GAGGATACATAACTTGAGGT+AGG 0.624081 4_4:+1061697 None:intergenic
GCTACAAATGAATCATCTGT+GGG 0.632745 4_4:-1062358 Msa0663000:CDS
ACCTGGTATGGTGACCCCGA+AGG 0.633830 4_4:-1065889 Msa0663000:CDS
TCAAGCCATTGAATTGCACA+AGG 0.633952 4_4:-1061974 Msa0663000:three_prime_UTR
ACCGCTGAGGTCAAAATGGG+TGG 0.634095 4_4:+1065344 None:intergenic
GAACTCCGTGTAAATATCCA+GGG 0.635650 4_4:-1064080 Msa0663000:CDS
GTCCAAAAGCAGCACCGCTG+AGG 0.643386 4_4:+1065331 None:intergenic
TGCCACTGAGGGATTCAGTG+TGG 0.647405 4_4:+1066273 None:intergenic
TTTACTGAAGTGTGCCACTG+AGG 0.648777 4_4:+1066261 None:intergenic
CGGTACTGCCGTCTCCTTCG+GGG 0.649859 4_4:+1065875 None:intergenic
GTTAAACTCAGCTCTACCAC+CGG 0.650609 4_4:-1062288 Msa0663000:CDS
TTTATGAAAGGGGAAGGATG+TGG 0.665300 4_4:-1065465 Msa0663000:CDS
ATATGAGGATACATAACTTG+AGG 0.665496 4_4:+1061693 None:intergenic
CTGAAGTTGTCCAACCGCGG+TGG 0.668940 4_4:+1065951 None:intergenic
CTCTGCCGACAGTTTCGTGG+CGG 0.672651 4_4:+1066094 None:intergenic
GTTGTTCGGAGCTGAAATGG+TGG 0.685072 4_4:+1066061 None:intergenic
GGGGTCACCATACCAGGTAG+CGG 0.688629 4_4:+1065894 None:intergenic
AACGTGTGTAGTGAGCCAGT+TGG 0.709559 4_4:-1061795 Msa0663000:three_prime_UTR
TTGTGGGTATGGAACATTGG+TGG 0.710549 4_4:-1065535 Msa0663000:CDS
AAACTGGAGAGAGAGCGTGT+AGG 0.712707 4_4:-1061846 Msa0663000:three_prime_UTR
AAGGGTCAACTAAGAAACCG+CGG 0.722148 4_4:-1065288 Msa0663000:CDS
GGTACAAAAGAAACCAACCA+AGG 0.722942 4_4:-1062162 Msa0663000:three_prime_UTR
CAAGCCATTGAATTGCACAA+GGG 0.732771 4_4:-1061973 Msa0663000:three_prime_UTR
AAATGACAGGGATTTGACCG+CGG 0.736984 4_4:+1065271 None:intergenic
ATGCTGAAGTTGTCCAACCG+CGG 0.752656 4_4:+1065948 None:intergenic
ACCCACACTGAATCCCTCAG+TGG 0.769287 4_4:-1066275 Msa0663000:five_prime_UTR
CTACAAATGAATCATCTGTG+GGG 0.791175 4_4:-1062357 Msa0663000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTACTTTTATGAATAT+TGG - chr4_4:1062740-1062759 Msa0663000:intron 10.0%
!! TAATTAATGTATTTATTTGT+TGG - chr4_4:1063449-1063468 Msa0663000:intron 10.0%
!! AATTAATGTATTTATTTGTT+GGG - chr4_4:1063450-1063469 Msa0663000:intron 10.0%
!!! TCTAATGTTTATTATTTTTT+TGG - chr4_4:1064049-1064068 Msa0663000:CDS 10.0%
!!! TAATATTAAGAAATTTTAAG+TGG - chr4_4:1064253-1064272 Msa0663000:intron 10.0%
!! TTTATAAATATGATGTTATT+TGG + chr4_4:1064432-1064451 None:intergenic 10.0%
!! TATTAATAACAAATTATGAA+TGG + chr4_4:1065394-1065413 None:intergenic 10.0%
!! TAGACAATGTATAATATATT+TGG - chr4_4:1062836-1062855 Msa0663000:intron 15.0%
!!! AATTACATAGTTTTTTTTGA+GGG + chr4_4:1062951-1062970 None:intergenic 15.0%
!!! AAATTACATAGTTTTTTTTG+AGG + chr4_4:1062952-1062971 None:intergenic 15.0%
!! TCATCAAATTTCAAAATTTA+AGG + chr4_4:1063067-1063086 None:intergenic 15.0%
!!! TAAACATATTTTTTTACACA+AGG - chr4_4:1063127-1063146 Msa0663000:intron 15.0%
!!! ATAAATCATTTTTCCTTAAA+AGG + chr4_4:1063700-1063719 None:intergenic 15.0%
!!! AGTTAAATATTTCCAAATTT+TGG - chr4_4:1064944-1064963 Msa0663000:intron 15.0%
!!! GTTAAATATTTCCAAATTTT+GGG - chr4_4:1064945-1064964 Msa0663000:intron 15.0%
!!! TTAAATATTTCCAAATTTTG+GGG - chr4_4:1064946-1064965 Msa0663000:intron 15.0%
!! CAAACTTTAATTAATTAACT+AGG + chr4_4:1065880-1065899 None:intergenic 15.0%
!! CTAGTTAATTAATTAAAGTT+TGG - chr4_4:1065878-1065897 Msa0663000:CDS 15.0%
!! TAATTAATTAAAGTTTGGTT+AGG - chr4_4:1065883-1065902 Msa0663000:CDS 15.0%
!! TAAAATTTAAAGACATTCAA+AGG + chr4_4:1066235-1066254 None:intergenic 15.0%
!!! TTCTAACTTTGAAGATTTTT+TGG + chr4_4:1063105-1063124 None:intergenic 20.0%
!!! TTTTAATATGCGTTTGATTA+AGG - chr4_4:1063405-1063424 Msa0663000:intron 20.0%
!! TTTAATATGCGTTTGATTAA+GGG - chr4_4:1063406-1063425 Msa0663000:intron 20.0%
!!! CATCTTAATTTTTTGTTCTT+TGG - chr4_4:1063631-1063650 Msa0663000:intron 20.0%
!!! TCTGTTTGTTTCTATAAAAT+AGG - chr4_4:1063735-1063754 Msa0663000:intron 20.0%
!! ATTTAATCCACAAAACAAAA+AGG + chr4_4:1063818-1063837 None:intergenic 20.0%
!! ATTCATTTAGATGTATCTTT+GGG + chr4_4:1064088-1064107 None:intergenic 20.0%
!! TATTCATTTAGATGTATCTT+TGG + chr4_4:1064089-1064108 None:intergenic 20.0%
!! AATAAAGAACAATTTGATAG+AGG - chr4_4:1064105-1064124 Msa0663000:intron 20.0%
!!! ATTAAGAAATTTTAAGTGGT+AGG - chr4_4:1064257-1064276 Msa0663000:intron 20.0%
!! TGTAATTGCATCATATTTAT+AGG + chr4_4:1064409-1064428 None:intergenic 20.0%
!! TTCAGTGTAATTATAAATGT+CGG + chr4_4:1064837-1064856 None:intergenic 20.0%
!! TTTCGAATTATTAGTTATGT+TGG - chr4_4:1064869-1064888 Msa0663000:intron 20.0%
!! ACACTTATACTCTATTTAAA+TGG + chr4_4:1065037-1065056 None:intergenic 20.0%
!!! GAAATGTTATTTAAAGATGT+AGG + chr4_4:1065158-1065177 None:intergenic 20.0%
!! ATGAATTATTTGATCTGATT+TGG + chr4_4:1065245-1065264 None:intergenic 20.0%
!!! AAATGCATCTTTTAAGATAT+TGG + chr4_4:1065322-1065341 None:intergenic 20.0%
!!! ATTGTATTTTTATTGCAGAA+TGG - chr4_4:1065586-1065605 Msa0663000:intron 20.0%
!! TTAATTAACTAGGTAAAATG+TGG + chr4_4:1065870-1065889 None:intergenic 20.0%
!! AGATATATATAGCAAACTAA+GGG + chr4_4:1065953-1065972 None:intergenic 20.0%
!! TAGATATATATAGCAAACTA+AGG + chr4_4:1065954-1065973 None:intergenic 20.0%
!!! TTGTATGCCTAATTTTATTA+TGG - chr4_4:1066070-1066089 Msa0663000:five_prime_UTR 20.0%
!! TAACACACCATAATAAAATT+AGG + chr4_4:1066080-1066099 None:intergenic 20.0%
! GTAAATAAACACAAAACCAA+CGG - chr4_4:1061736-1061755 Msa0663000:three_prime_UTR 25.0%
!! TTTATCTATAAAGCACTCAA+CGG + chr4_4:1062274-1062293 None:intergenic 25.0%
! AGATAAATACTCAATGTATC+TGG - chr4_4:1062287-1062306 Msa0663000:CDS 25.0%
! CTCAAGTTTCTCTTATAAAT+AGG - chr4_4:1062336-1062355 Msa0663000:CDS 25.0%
!!! GAATATTGGTTTATTCTTTC+TGG - chr4_4:1062754-1062773 Msa0663000:intron 25.0%
!! TTTTTCCTATGTCATATTAG+CGG - chr4_4:1062869-1062888 Msa0663000:intron 25.0%
! AACATATATTAACCCGATAT+AGG - chr4_4:1063181-1063200 Msa0663000:intron 25.0%
!! AGTAACAAAACAAATTTGCT+AGG + chr4_4:1063377-1063396 None:intergenic 25.0%
!!! AGCATGATTGGTTAATTTTT+TGG - chr4_4:1063511-1063530 Msa0663000:intron 25.0%
!!! ATGATTGGTTAATTTTTTGG+TGG - chr4_4:1063514-1063533 Msa0663000:intron 25.0%
!!! TGATTGGTTAATTTTTTGGT+GGG - chr4_4:1063515-1063534 Msa0663000:intron 25.0%
! AAAGAGTCAATATACAACAA+AGG + chr4_4:1063559-1063578 None:intergenic 25.0%
! AAATGTTTGCACCATATAAA+AGG + chr4_4:1063602-1063621 None:intergenic 25.0%
! ACTTTGTTGAATCATATGAT+AGG - chr4_4:1063778-1063797 Msa0663000:intron 25.0%
!!! TAATGTACCTTTTTGTTTTG+TGG - chr4_4:1063808-1063827 Msa0663000:intron 25.0%
! ATTGCATTTCATGTTAATGA+AGG - chr4_4:1063928-1063947 Msa0663000:intron 25.0%
!!! AAATTTTGACTGGAATTAGT+TGG - chr4_4:1064642-1064661 Msa0663000:intron 25.0%
!! AAACGTGAAATAAAGCATTA+TGG - chr4_4:1064667-1064686 Msa0663000:intron 25.0%
!! TGATTTTCCATGATAGTAAA+AGG - chr4_4:1064707-1064726 Msa0663000:intron 25.0%
!!! TATACATCAAAAGCACTAAA+GGG + chr4_4:1065091-1065110 None:intergenic 25.0%
!!! ATATACATCAAAAGCACTAA+AGG + chr4_4:1065092-1065111 None:intergenic 25.0%
! TCTAACTTTACTAGATTTAG+AGG + chr4_4:1065349-1065368 None:intergenic 25.0%
! AATATCCAATCATTGGTAAA+TGG + chr4_4:1065440-1065459 None:intergenic 25.0%
!!! TGTTTTAGTGGTAGAAATTT+AGG + chr4_4:1065783-1065802 None:intergenic 25.0%
!!! GTGAACTATTTTTACGTTAT+GGG + chr4_4:1065910-1065929 None:intergenic 25.0%
!!! TGTGAACTATTTTTACGTTA+TGG + chr4_4:1065911-1065930 None:intergenic 25.0%
! TAACGTAAAAATAGTTCACA+TGG - chr4_4:1065911-1065930 Msa0663000:CDS 25.0%
! AACGTAAAAATAGTTCACAT+GGG - chr4_4:1065912-1065931 Msa0663000:CDS 25.0%
! TTTCTTACAAAGAGAAAAAG+TGG + chr4_4:1065980-1065999 None:intergenic 25.0%
!! TTATTATGGTGTGTTATTGT+TGG - chr4_4:1066084-1066103 Msa0663000:five_prime_UTR 25.0%
! ATTTAAAGACATTCAAAGGT+TGG + chr4_4:1066231-1066250 None:intergenic 25.0%
ATCATACTCTTGACTTACAT+TGG - chr4_4:1062052-1062071 Msa0663000:three_prime_UTR 30.0%
CTTCTGATTAAAATCGTGTT+TGG - chr4_4:1062181-1062200 Msa0663000:three_prime_UTR 30.0%
GTTTCTCTTATAAATAGGTC+CGG - chr4_4:1062341-1062360 Msa0663000:CDS 30.0%
ATTACAACTAATACTCACTC+CGG + chr4_4:1062363-1062382 None:intergenic 30.0%
GTTAATAACAGTGAGTATGT+TGG - chr4_4:1062383-1062402 Msa0663000:intron 30.0%
ATGATGAGAATGTGTGAATT+AGG - chr4_4:1062415-1062434 Msa0663000:intron 30.0%
TAGACAATAGCATATGTTCA+AGG - chr4_4:1062560-1062579 Msa0663000:intron 30.0%
TTTACCTTCTGTAAATGACA+GGG + chr4_4:1062723-1062742 None:intergenic 30.0%
! TTTTACCTTCTGTAAATGAC+AGG + chr4_4:1062724-1062743 None:intergenic 30.0%
! CTTTTATTCTCTGTAAAACC+AGG - chr4_4:1062788-1062807 Msa0663000:intron 30.0%
AAAAACCGCTAATATGACAT+AGG + chr4_4:1062877-1062896 None:intergenic 30.0%
! TATGTCATATTAGCGGTTTT+TGG - chr4_4:1062876-1062895 Msa0663000:intron 30.0%
!!! GATTGGTTAATTTTTTGGTG+GGG - chr4_4:1063516-1063535 Msa0663000:intron 30.0%
! TGTTCCTTTCTCCTTTTATA+TGG - chr4_4:1063588-1063607 Msa0663000:intron 30.0%
TATAGAAACAAACAGAGTAG+TGG + chr4_4:1063731-1063750 None:intergenic 30.0%
! TTTTTCCCTGGATATTTACA+CGG + chr4_4:1063907-1063926 None:intergenic 30.0%
!!! TGAAATGCAATTTTTTTCCC+TGG + chr4_4:1063919-1063938 None:intergenic 30.0%
ACTAGAAGTGATAACCAAAA+TGG + chr4_4:1063961-1063980 None:intergenic 30.0%
! AAGTATTGCATTTGCATCTT+TGG - chr4_4:1064594-1064613 Msa0663000:intron 30.0%
!! AAACTGTTGCAAATTTTGAC+TGG - chr4_4:1064632-1064651 Msa0663000:intron 30.0%
! TAAAGCATTATGGTCATTCA+CGG - chr4_4:1064677-1064696 Msa0663000:intron 30.0%
! AGTAGTTCCTTTTACTATCA+TGG + chr4_4:1064717-1064736 None:intergenic 30.0%
CTATTTAAATGGTGTGAAGA+GGG + chr4_4:1065026-1065045 None:intergenic 30.0%
TCTATTTAAATGGTGTGAAG+AGG + chr4_4:1065027-1065046 None:intergenic 30.0%
GAAGTTGGGAATATAATATG+TGG + chr4_4:1065068-1065087 None:intergenic 30.0%
!! ATACATCAAAAGCACTAAAG+GGG + chr4_4:1065090-1065109 None:intergenic 30.0%
TTAGTCCATTTACCAATGAT+TGG - chr4_4:1065432-1065451 Msa0663000:CDS 30.0%
TGTGAGTAATATCCAATCAT+TGG + chr4_4:1065447-1065466 None:intergenic 30.0%
TAAAACACAACCTTTGCAAT+TGG - chr4_4:1065796-1065815 Msa0663000:intron 30.0%
! TTCTTTTGTACCAATTGCAA+AGG + chr4_4:1065809-1065828 None:intergenic 30.0%
ATTAATAACCCACTATAGGA+AGG + chr4_4:1065845-1065864 None:intergenic 30.0%
GGTTATTAATAACCCACTAT+AGG + chr4_4:1065849-1065868 None:intergenic 30.0%
AAGAGAAACAAGAAAAGAGA+AGG + chr4_4:1066043-1066062 None:intergenic 30.0%
!! AGTATTCTTTAAAGTCCAAC+TGG + chr4_4:1066202-1066221 None:intergenic 30.0%
CATTGTACACTACTGTTGTT+CGG + chr4_4:1061935-1061954 None:intergenic 35.0%
TAAGAGAGAGATGTTGTTAC+CGG + chr4_4:1062127-1062146 None:intergenic 35.0%
ATGAGAATGTGTGAATTAGG+TGG - chr4_4:1062418-1062437 Msa0663000:intron 35.0%
! AAATCCCTGTCATTTACAGA+AGG - chr4_4:1062716-1062735 Msa0663000:intron 35.0%
ATTCTCTGTAAAACCAGGAT+AGG - chr4_4:1062793-1062812 Msa0663000:intron 35.0%
TTCTCTGTAAAACCAGGATA+GGG - chr4_4:1062794-1062813 Msa0663000:intron 35.0%
AGCGAGAGATAAACCAAAAT+TGG - chr4_4:1063027-1063046 Msa0663000:intron 35.0%
GAGAGATAAACCAAAATTGG+CGG - chr4_4:1063030-1063049 Msa0663000:intron 35.0%
TATTAACCCGATATAGGAGT+CGG - chr4_4:1063187-1063206 Msa0663000:intron 35.0%
TTTGTGCCCAATGAATTCTT+TGG - chr4_4:1063301-1063320 Msa0663000:intron 35.0%
AGTGTGCCAAAGAATTCATT+GGG + chr4_4:1063310-1063329 None:intergenic 35.0%
TAGTGTGCCAAAGAATTCAT+TGG + chr4_4:1063311-1063330 None:intergenic 35.0%
TGCACCATATAAAAGGAGAA+AGG + chr4_4:1063595-1063614 None:intergenic 35.0%
AAGTGATAACCAAAATGGTG+TGG + chr4_4:1063956-1063975 None:intergenic 35.0%
AGTTGAATTTGAAGATGCAG+AGG - chr4_4:1063978-1063997 Msa0663000:CDS 35.0%
GTTGAATTTGAAGATGCAGA+GGG - chr4_4:1063979-1063998 Msa0663000:CDS 35.0%
AACAATTTGATAGAGGCAGA+TGG - chr4_4:1064112-1064131 Msa0663000:intron 35.0%
TCAGATGCAATTACAGCTAA+CGG - chr4_4:1064226-1064245 Msa0663000:intron 35.0%
TTGAAGTCCTTTCATAGAGT+GGG + chr4_4:1065195-1065214 None:intergenic 35.0%
ACTTCAAGCTTAAGAACACT+TGG - chr4_4:1065208-1065227 Msa0663000:intron 35.0%
GCTACAAATGAATCATCTGT+GGG - chr4_4:1065621-1065640 Msa0663000:intron 35.0%
CTACAAATGAATCATCTGTG+GGG - chr4_4:1065622-1065641 Msa0663000:intron 35.0%
! CATTATTGGTGGACCTTTAA+GGG - chr4_4:1065657-1065676 Msa0663000:intron 35.0%
! ATTATTGGTGGACCTTTAAG+GGG - chr4_4:1065658-1065677 Msa0663000:intron 35.0%
AACAACATCTCTAGCTGATA+GGG + chr4_4:1065722-1065741 None:intergenic 35.0%
AGATGTTGTTCCTAGCAATT+GGG - chr4_4:1065732-1065751 Msa0663000:intron 35.0%
!! TTGCAAAGGTTGTGTTTTAG+TGG + chr4_4:1065795-1065814 None:intergenic 35.0%
AAACTAAGGGAAAGAGAGTT+GGG + chr4_4:1065940-1065959 None:intergenic 35.0%
!! AATTGCACAAGGGCATTAAA+TGG - chr4_4:1066016-1066035 Msa0663000:CDS 35.0%
! TTATGGTGTGTTATTGTTGG+TGG - chr4_4:1066087-1066106 Msa0663000:five_prime_UTR 35.0%
TTCAACTCTGCAAGGAATTA+AGG + chr4_4:1066166-1066185 None:intergenic 35.0%
CACAAAACCAACGGACAAAA+AGG - chr4_4:1061745-1061764 Msa0663000:three_prime_UTR 40.0%
! CTTTTGACCTTTTTGTCCGT+TGG + chr4_4:1061755-1061774 None:intergenic 40.0%
! TCCAATTTAGCTCCTATCTG+AGG - chr4_4:1061959-1061978 Msa0663000:three_prime_UTR 40.0%
!! TATCTGAGGCTTTTCTGCTT+CGG - chr4_4:1061973-1061992 Msa0663000:three_prime_UTR 40.0%
CTTGACTTACATTGGTATCC+CGG - chr4_4:1062060-1062079 Msa0663000:three_prime_UTR 40.0%
!! GACCAGTGCTGTTTATGAAA+GGG - chr4_4:1062503-1062522 Msa0663000:intron 40.0%
!! ACCAGTGCTGTTTATGAAAG+GGG - chr4_4:1062504-1062523 Msa0663000:intron 40.0%
TTTATGAAAGGGGAAGGATG+TGG - chr4_4:1062514-1062533 Msa0663000:intron 40.0%
TTTGGACATATGGCTATCTC+CGG - chr4_4:1062664-1062683 Msa0663000:intron 40.0%
CAAAAAGAACTACCCTATCC+TGG + chr4_4:1062809-1062828 None:intergenic 40.0%
! CAGGATAGGGTAGTTCTTTT+TGG - chr4_4:1062807-1062826 Msa0663000:intron 40.0%
TCAAACTCCGACTCCTATAT+CGG + chr4_4:1063197-1063216 None:intergenic 40.0%
ACCTGACATTGATATGCTGA+AGG - chr4_4:1063484-1063503 Msa0663000:intron 40.0%
TCCTTCAGCATATCAATGTC+AGG + chr4_4:1063488-1063507 None:intergenic 40.0%
! CGTAGCTCAGTATCCTTTTA+AGG - chr4_4:1063684-1063703 Msa0663000:intron 40.0%
AGAACTCCGTGTAAATATCC+AGG - chr4_4:1063898-1063917 Msa0663000:intron 40.0%
GAACTCCGTGTAAATATCCA+GGG - chr4_4:1063899-1063918 Msa0663000:intron 40.0%
! ATGAAGGTTCCACACCATTT+TGG - chr4_4:1063944-1063963 Msa0663000:intron 40.0%
TCTGAGAGATCAGAAGTTCA+GGG + chr4_4:1064203-1064222 None:intergenic 40.0%
TATAAATGTCGGCGTTGTGT+CGG + chr4_4:1064826-1064845 None:intergenic 40.0%
ATACAGACATGGACATGACA+TGG + chr4_4:1064906-1064925 None:intergenic 40.0%
TGTCTCACTCTTAGGCAATT+GGG - chr4_4:1064995-1065014 Msa0663000:intron 40.0%
! AAAGCACTAAAGGGGAAGTT+GGG + chr4_4:1065082-1065101 None:intergenic 40.0%
!! AAAAGCACTAAAGGGGAAGT+TGG + chr4_4:1065083-1065102 None:intergenic 40.0%
TGCACTCAATGCATGTGATA+TGG - chr4_4:1065130-1065149 Msa0663000:intron 40.0%
GCACTCAATGCATGTGATAT+GGG - chr4_4:1065131-1065150 Msa0663000:intron 40.0%
TGAAGTCCTTTCATAGAGTG+GGG + chr4_4:1065194-1065213 None:intergenic 40.0%
CTTGAAGTCCTTTCATAGAG+TGG + chr4_4:1065196-1065215 None:intergenic 40.0%
GGCTACAAATGAATCATCTG+TGG - chr4_4:1065620-1065639 Msa0663000:intron 40.0%
GGTGCAAATTGGTGCATTAT+TGG - chr4_4:1065643-1065662 Msa0663000:intron 40.0%
GCAAATTGGTGCATTATTGG+TGG - chr4_4:1065646-1065665 Msa0663000:intron 40.0%
! GCATTATTGGTGGACCTTTA+AGG - chr4_4:1065656-1065675 Msa0663000:intron 40.0%
GAGCTGAGTTTAACAGAGAA+TGG + chr4_4:1065685-1065704 None:intergenic 40.0%
GAACAACATCTCTAGCTGAT+AGG + chr4_4:1065723-1065742 None:intergenic 40.0%
GAGATGTTGTTCCTAGCAAT+TGG - chr4_4:1065731-1065750 Msa0663000:intron 40.0%
! ATTTAGGCGAGAAGTGTAAG+TGG + chr4_4:1065767-1065786 None:intergenic 40.0%
GGTACAAAAGAAACCAACCA+AGG - chr4_4:1065817-1065836 Msa0663000:intron 40.0%
CAAACTAAGGGAAAGAGAGT+TGG + chr4_4:1065941-1065960 None:intergenic 40.0%
TCAAGCCATTGAATTGCACA+AGG - chr4_4:1066005-1066024 Msa0663000:CDS 40.0%
CAAGCCATTGAATTGCACAA+GGG - chr4_4:1066006-1066025 Msa0663000:CDS 40.0%
AATGCCCTTGTGCAATTCAA+TGG + chr4_4:1066013-1066032 None:intergenic 40.0%
GAGGATACATAACTTGAGGT+AGG + chr4_4:1066285-1066304 None:intergenic 40.0%
! TTACTGAAGTGTGCCACTGA+GGG + chr4_4:1061720-1061739 None:intergenic 45.0%
! TTTACTGAAGTGTGCCACTG+AGG + chr4_4:1061721-1061740 None:intergenic 45.0%
CCAACAACCCTCTCTTCTAA+CGG - chr4_4:1061804-1061823 Msa0663000:three_prime_UTR 45.0%
GGTTGAACCGTTAGAAGAGA+GGG + chr4_4:1061814-1061833 None:intergenic 45.0%
AGGTTGAACCGTTAGAAGAG+AGG + chr4_4:1061815-1061834 None:intergenic 45.0%
TTCTGTCTGTTGTGGCGTTT+GGG + chr4_4:1061841-1061860 None:intergenic 45.0%
ATTCTGTCTGTTGTGGCGTT+TGG + chr4_4:1061842-1061861 None:intergenic 45.0%
ACTGTTGTTCGGAGCTGAAA+TGG + chr4_4:1061924-1061943 None:intergenic 45.0%
GCCTCAGATAGGAGCTAAAT+TGG + chr4_4:1061963-1061982 None:intergenic 45.0%
GAAGCAGAAAAGCCTCAGAT+AGG + chr4_4:1061974-1061993 None:intergenic 45.0%
TGTGAATTAGGTGGAGCTTG+TGG - chr4_4:1062427-1062446 Msa0663000:intron 45.0%
GTGAATTAGGTGGAGCTTGT+GGG - chr4_4:1062428-1062447 Msa0663000:intron 45.0%
AGCTTGTGGGTATGGAACAT+TGG - chr4_4:1062441-1062460 Msa0663000:intron 45.0%
TTGTGGGTATGGAACATTGG+TGG - chr4_4:1062444-1062463 Msa0663000:intron 45.0%
!! GGACCAGTGCTGTTTATGAA+AGG - chr4_4:1062502-1062521 Msa0663000:intron 45.0%
TCCCCTTTCATAAACAGCAC+TGG + chr4_4:1062508-1062527 None:intergenic 45.0%
!! GTGCTGTTTATGAAAGGGGA+AGG - chr4_4:1062508-1062527 Msa0663000:intron 45.0%
! CAAAATGGGTGGTGTCAGTT+GGG + chr4_4:1062627-1062646 None:intergenic 45.0%
! TCAAAATGGGTGGTGTCAGT+TGG + chr4_4:1062628-1062647 None:intergenic 45.0%
TCTTAGTTGACCCTTCTCAC+CGG + chr4_4:1062686-1062705 None:intergenic 45.0%
AAGGGTCAACTAAGAAACCG+CGG - chr4_4:1062691-1062710 Msa0663000:intron 45.0%
AAATGACAGGGATTTGACCG+CGG + chr4_4:1062711-1062730 None:intergenic 45.0%
! ACGTTTGTCACCGCCAATTT+TGG + chr4_4:1063043-1063062 None:intergenic 45.0%
CAAACTCCGACTCCTATATC+GGG + chr4_4:1063196-1063215 None:intergenic 45.0%
! GCTGAAGGAAGAAGCATGAT+TGG - chr4_4:1063499-1063518 Msa0663000:intron 45.0%
CGGAGTTCTGCATTTCACAT+AGG + chr4_4:1063887-1063906 None:intergenic 45.0%
GAAGATGCAGAGGGAGATAT+TGG - chr4_4:1063988-1064007 Msa0663000:CDS 45.0%
! CTCTGAGAGATCAGAAGTTC+AGG + chr4_4:1064204-1064223 None:intergenic 45.0%
GTGAAGCACGAATACAGACA+TGG + chr4_4:1064917-1064936 None:intergenic 45.0%
! ACAGAGGCACGTGATTTAAG+AGG + chr4_4:1064978-1064997 None:intergenic 45.0%
ATTGCCTAAGAGTGAGACAG+AGG + chr4_4:1064994-1065013 None:intergenic 45.0%
CTGTCTCACTCTTAGGCAAT+TGG - chr4_4:1064994-1065013 Msa0663000:intron 45.0%
TTTCTCCCCCACTCTATGAA+AGG - chr4_4:1065185-1065204 Msa0663000:intron 45.0%
GAAGTCCTTTCATAGAGTGG+GGG + chr4_4:1065193-1065212 None:intergenic 45.0%
!! TGCAGAATGGTTCTAGTGAG+TGG - chr4_4:1065599-1065618 Msa0663000:intron 45.0%
ATCATCTGTGGGGTGCAAAT+TGG - chr4_4:1065632-1065651 Msa0663000:intron 45.0%
ACAGAGAATGGTCCCCTTAA+AGG + chr4_4:1065673-1065692 None:intergenic 45.0%
GTTAAACTCAGCTCTACCAC+CGG - chr4_4:1065691-1065710 Msa0663000:intron 45.0%
TCTAGCTGATAGGGTTCTAC+CGG + chr4_4:1065713-1065732 None:intergenic 45.0%
TCCTAGCAATTGGGTTCCAA+AGG - chr4_4:1065741-1065760 Msa0663000:intron 45.0%
!!! TCTCTCTCCAGTTTTGGAGA+GGG + chr4_4:1066127-1066146 None:intergenic 45.0%
TACACACGTTCAACTCTGCA+AGG + chr4_4:1066174-1066193 None:intergenic 45.0%
!! ATTTCAATAAATAAAAAAAA+AGG + chr4_4:1063249-1063268 None:intergenic 5.0%
!! ATTAAAATAAAAATATTACA+TGG + chr4_4:1064342-1064361 None:intergenic 5.0%
CCGTTAGAAGAGAGGGTTGT+TGG + chr4_4:1061807-1061826 None:intergenic 50.0%
! CTGTTGTGGCGTTTGGGTTT+AGG + chr4_4:1061835-1061854 None:intergenic 50.0%
GAGGGAGATTCTGTCTGTTG+TGG + chr4_4:1061849-1061868 None:intergenic 50.0%
GTTGTTCGGAGCTGAAATGG+TGG + chr4_4:1061921-1061940 None:intergenic 50.0%
ATGCTGAAGTTGTCCAACCG+CGG + chr4_4:1062034-1062053 None:intergenic 50.0%
TTAGGTGGAGCTTGTGGGTA+TGG - chr4_4:1062433-1062452 Msa0663000:intron 50.0%
ATAATCACTGACGAGTGCCC+AGG - chr4_4:1062598-1062617 Msa0663000:intron 50.0%
!! ACCACCCATTTTGACCTCAG+CGG - chr4_4:1062634-1062653 Msa0663000:intron 50.0%
AGCACCGCTGAGGTCAAAAT+GGG + chr4_4:1062641-1062660 None:intergenic 50.0%
!!! CGGTGCTGCTTTTGGACATA+TGG - chr4_4:1062654-1062673 Msa0663000:intron 50.0%
TATGGCTATCTCCGGTGAGA+AGG - chr4_4:1062672-1062691 Msa0663000:intron 50.0%
ATGGCTATCTCCGGTGAGAA+GGG - chr4_4:1062673-1062692 Msa0663000:intron 50.0%
GAGGACACGTCCCCAAAATT+TGG + chr4_4:1064959-1064978 None:intergenic 50.0%
!! GCCTTTGGAACCCAATTGCT+AGG + chr4_4:1065745-1065764 None:intergenic 50.0%
! CGAGAAGTGTAAGTGGCCTT+TGG + chr4_4:1065760-1065779 None:intergenic 50.0%
CTATAGGAAGGAGCCTTGGT+TGG + chr4_4:1065833-1065852 None:intergenic 50.0%
ACCAAGGCTCCTTCCTATAG+TGG - chr4_4:1065833-1065852 Msa0663000:intron 50.0%
CCAAGGCTCCTTCCTATAGT+GGG - chr4_4:1065834-1065853 Msa0663000:intron 50.0%
CATGTAGCCCTCTCCAAAAC+TGG - chr4_4:1066117-1066136 Msa0663000:five_prime_UTR 50.0%
!!! CTCTCTCTCCAGTTTTGGAG+AGG + chr4_4:1066128-1066147 None:intergenic 50.0%
! ACACGCTCTCTCTCCAGTTT+TGG + chr4_4:1066133-1066152 None:intergenic 50.0%
! AAACTGGAGAGAGAGCGTGT+AGG - chr4_4:1066133-1066152 Msa0663000:five_prime_UTR 50.0%
! AACTGGAGAGAGAGCGTGTA+GGG - chr4_4:1066134-1066153 Msa0663000:five_prime_UTR 50.0%
AACGTGTGTAGTGAGCCAGT+TGG - chr4_4:1066184-1066203 Msa0663000:five_prime_UTR 50.0%
ACCCACACTGAATCCCTCAG+TGG - chr4_4:1061704-1061723 Msa0663000:three_prime_UTR 55.0%
GCCACTGAGGGATTCAGTGT+GGG + chr4_4:1061708-1061727 None:intergenic 55.0%
TGCCACTGAGGGATTCAGTG+TGG + chr4_4:1061709-1061728 None:intergenic 55.0%
GGCAGAGAGAGCAAGAGAGA+GGG + chr4_4:1061867-1061886 None:intergenic 55.0%
! ACCATACCAGGTAGCGGTAC+CGG + chr4_4:1062082-1062101 None:intergenic 55.0%
TATGGTGACCCCGAAGGAGA+CGG - chr4_4:1062096-1062115 Msa0663000:three_prime_UTR 55.0%
! TGGTGGACGTGAAGCCATTG+AGG - chr4_4:1062461-1062480 Msa0663000:intron 55.0%
GGTGGACGTGAAGCCATTGA+GGG - chr4_4:1062462-1062481 Msa0663000:intron 55.0%
ACCGCTGAGGTCAAAATGGG+TGG + chr4_4:1062638-1062657 None:intergenic 55.0%
CAGCACCGCTGAGGTCAAAA+TGG + chr4_4:1062642-1062661 None:intergenic 55.0%
CGTGCCTCTGTCTCACTCTT+AGG - chr4_4:1064987-1065006 Msa0663000:intron 55.0%
AGCTGATAGGGTTCTACCGG+TGG + chr4_4:1065710-1065729 None:intergenic 55.0%
CCCACTATAGGAAGGAGCCT+TGG + chr4_4:1065837-1065856 None:intergenic 55.0%
CGGCAGAGAGAGCAAGAGAG+AGG + chr4_4:1061868-1061887 None:intergenic 60.0%
CTCTGCCGCCACGAAACTGT+CGG - chr4_4:1061880-1061899 Msa0663000:three_prime_UTR 60.0%
CTCTGCCGACAGTTTCGTGG+CGG + chr4_4:1061888-1061907 None:intergenic 60.0%
GAGCTCTGCCGACAGTTTCG+TGG + chr4_4:1061891-1061910 None:intergenic 60.0%
CATGAAGCTCGCCCTGCTTC+CGG - chr4_4:1062002-1062021 Msa0663000:three_prime_UTR 60.0%
CTGAAGTTGTCCAACCGCGG+TGG + chr4_4:1062031-1062050 None:intergenic 60.0%
! CCATACCAGGTAGCGGTACC+GGG + chr4_4:1062081-1062100 None:intergenic 60.0%
GGGGTCACCATACCAGGTAG+CGG + chr4_4:1062088-1062107 None:intergenic 60.0%
ACCTGGTATGGTGACCCCGA+AGG - chr4_4:1062090-1062109 Msa0663000:three_prime_UTR 60.0%
TCCTTCGGGGTCACCATACC+AGG + chr4_4:1062094-1062113 None:intergenic 60.0%
ACCGGTACTGCCGTCTCCTT+CGG + chr4_4:1062109-1062128 None:intergenic 60.0%
ACGTGAAGCCATTGAGGGCG+AGG - chr4_4:1062467-1062486 Msa0663000:intron 60.0%
CGTGAAGCCATTGAGGGCGA+GGG - chr4_4:1062468-1062487 Msa0663000:intron 60.0%
AAGCCATTGAGGGCGAGGGT+AGG - chr4_4:1062472-1062491 Msa0663000:intron 60.0%
AGCCATTGAGGGCGAGGGTA+GGG - chr4_4:1062473-1062492 Msa0663000:intron 60.0%
! TGGTGTCAGTTGGGCAGCCT+GGG + chr4_4:1062618-1062637 None:intergenic 60.0%
!!! GACCTCAGCGGTGCTGCTTT+TGG - chr4_4:1062646-1062665 Msa0663000:intron 60.0%
GTCCAAAAGCAGCACCGCTG+AGG + chr4_4:1062651-1062670 None:intergenic 60.0%
! TGTCGGCGTTGTGTCGGTGT+CGG + chr4_4:1064820-1064839 None:intergenic 60.0%
CGGAAGCAGGGCGAGCTTCA+TGG + chr4_4:1062004-1062023 None:intergenic 65.0%
GGTATCCCGGTACCGCTACC+TGG - chr4_4:1062073-1062092 Msa0663000:three_prime_UTR 65.0%
CCCGGTACCGCTACCTGGTA+TGG - chr4_4:1062078-1062097 Msa0663000:three_prime_UTR 65.0%
CGGTACTGCCGTCTCCTTCG+GGG + chr4_4:1062107-1062126 None:intergenic 65.0%
CCGGTACTGCCGTCTCCTTC+GGG + chr4_4:1062108-1062127 None:intergenic 65.0%
CCCGAAGGAGACGGCAGTAC+CGG - chr4_4:1062105-1062124 Msa0663000:three_prime_UTR 65.0%
GCCATTGAGGGCGAGGGTAG+GGG - chr4_4:1062474-1062493 Msa0663000:intron 65.0%
! GTGGTGTCAGTTGGGCAGCC+TGG + chr4_4:1062619-1062638 None:intergenic 65.0%
TGTCCAACCGCGGTGGCAGC+CGG + chr4_4:1062024-1062043 None:intergenic 70.0%
GCCCCTACCCTCGCCCTCAA+TGG + chr4_4:1062478-1062497 None:intergenic 70.0%
AGGGCGAGGGTAGGGGCAGT+GGG - chr4_4:1062481-1062500 Msa0663000:intron 70.0%
CGCGGTGGCAGCCGGAAGCA+GGG + chr4_4:1062016-1062035 None:intergenic 75.0%
CCTGCTTCCGGCTGCCACCG+CGG - chr4_4:1062014-1062033 Msa0663000:three_prime_UTR 75.0%
CTTCCGGCTGCCACCGCGGT+TGG - chr4_4:1062018-1062037 Msa0663000:three_prime_UTR 75.0%
GAGGGCGAGGGTAGGGGCAG+TGG - chr4_4:1062480-1062499 Msa0663000:intron 75.0%
! CCGCGGTGGCAGCCGGAAGC+AGG + chr4_4:1062017-1062036 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 1061697 1066304 1061697 ID=Msa0663000;Name=Msa0663000
chr4_4 mRNA 1061697 1066304 1061697 ID=Msa0663000-mRNA-1;Parent=Msa0663000;Name=Msa0663000-mRNA-1;_AED=0.03;_eAED=0.03;_QI=255|1|1|1|1|1|4|506|266
chr4_4 exon 1061697 1062397 1061697 ID=Msa0663000-mRNA-1:exon:388;Parent=Msa0663000-mRNA-1
chr4_4 exon 1063972 1064101 1063972 ID=Msa0663000-mRNA-1:exon:387;Parent=Msa0663000-mRNA-1
chr4_4 exon 1065264 1065564 1065264 ID=Msa0663000-mRNA-1:exon:386;Parent=Msa0663000-mRNA-1
chr4_4 exon 1065875 1066304 1065875 ID=Msa0663000-mRNA-1:exon:385;Parent=Msa0663000-mRNA-1
chr4_4 five_prime_UTR 1066050 1066304 1066050 ID=Msa0663000-mRNA-1:five_prime_utr;Parent=Msa0663000-mRNA-1
chr4_4 CDS 1065875 1066049 1065875 ID=Msa0663000-mRNA-1:cds;Parent=Msa0663000-mRNA-1
chr4_4 CDS 1065264 1065564 1065264 ID=Msa0663000-mRNA-1:cds;Parent=Msa0663000-mRNA-1
chr4_4 CDS 1063972 1064101 1063972 ID=Msa0663000-mRNA-1:cds;Parent=Msa0663000-mRNA-1
chr4_4 CDS 1062203 1062397 1062203 ID=Msa0663000-mRNA-1:cds;Parent=Msa0663000-mRNA-1
chr4_4 three_prime_UTR 1061697 1062202 1061697 ID=Msa0663000-mRNA-1:three_prime_utr;Parent=Msa0663000-mRNA-1
Gene Sequence

>Msa0663000

ATGCAGCTCCAATTTAGCTCCTATCTGAGGCTTTTCTGCTTCGGCTTAGCCATGAAGCTCGCCCTGCTTCCGGCTGCCACCGCGGTTGGACAACTTCAGCATCATACTCTTGACTTACATTGGTATCCCGGTACCGCTACCTGGTATGGTGACCCCGAAGGAGACGGCAGTACCGGTGGAGCTTGTGGGTATGGAACATTGGTGGACGTGAAGCCATTGAGGGCGAGGGTAGGGGCAGTGGGACCAGTGCTGTTTATGAAAGGGGAAGGATGTGGTGCTTGCTATAAAGTGAAGTGTTTAGACAATAGCATATGTTCAAGGAGAGCAGTTACTGTGATAATCACTGACGAGTGCCCAGGCTGCCCAACTGACACCACCCATTTTGACCTCAGCGGTGCTGCTTTTGGACATATGGCTATCTCCGGTGAGAAGGGTCAACTAAGAAACCGCGGTCAAATCCCTGTCATTTACAGAAGAACTCCGTGTAAATATCCAGGGAAAAAAATTGCATTTCATGTTAATGAAGGTTCCACACCATTTTGGTTATCACTTCTAGTTGAATTTGAAGATGCAGAGGGAGATATTGGCACTATGCATATACGAGAAAATGGTTCTAGTGAGTGGCTACAAATGAATCATCTGTGGGGTGCAAATTGGTGCATTATTGGTGGACCTTTAAGGGGACCATTCTCTGTTAAACTCAGCTCTACCACCGGTAGAACCCTATCAGCTAGAGATGTTGTTCCTAGCAATTGGGTTCCAAAGGCCACTTACACTTCTCGCCTAAATTTCTACCACTAA
Protein sequence

>Msa0663000

MQLQFSSYLRLFCFGLAMKLALLPAATAVGQLQHHTLDLHWYPGTATWYGDPEGDGSTGGACGYGTLVDVKPLRARVGAVGPVLFMKGEGCGACYKVKCLDNSICSRRAVTVIITDECPGCPTDTTHFDLSGAAFGHMAISGEKGQLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHIRENGSSEWLQMNHLWGANWCIIGGPLRGPFSVKLSSTTGRTLSARDVVPSNWVPKATYTSRLNFYH*