AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0678080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678080 MtrunA17_Chr4g0059761 83.333 78 13 0 28 105 740 817 2.27e-38 139
Msa0678080 MtrunA17_Chr5g0433601 90.323 62 4 1 114 173 179 240 1.53e-32 120
Msa0678080 MtrunA17_Chr3g0095661 74.194 62 14 1 114 173 138 199 5.56e-27 104
Msa0678080 MtrunA17_Chr5g0442671 69.355 62 17 1 114 173 131 192 8.92e-24 95.9
Msa0678080 MtrunA17_Chr3g0121311 59.677 62 23 1 114 173 127 188 6.94e-21 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678080 AT5G62600.1 77.143 70 16 0 28 97 736 805 2.42e-32 122
Msa0678080 AT5G17230.3 77.419 62 12 1 114 173 182 243 1.94e-27 107
Msa0678080 AT5G17230.2 77.419 62 12 1 114 173 167 228 2.06e-27 106
Msa0678080 AT5G17230.1 77.419 62 12 1 114 173 167 228 2.06e-27 106
Msa0678080 AT5G17230.4 77.419 62 12 1 114 173 167 228 2.06e-27 106

Find 100 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTAATTATGCAGGATTT+TGG 0.187064 4_4:-32854579 Msa0678080:intron
GAGAGAAGAAATTGTCAATT+TGG 0.207441 4_4:+32857663 None:intergenic
ATTGAATGTTCTTGTTTCTT+TGG 0.213959 4_4:-32856489 Msa0678080:exon
AATGTTACTGTTCTTAATTA+TGG 0.293181 4_4:-32856783 Msa0678080:five_prime_UTR
GAAGGTCTATCTGGATTGTT+TGG 0.313321 4_4:+32857030 None:intergenic
CTGTAGTGAGTGAGTGAGAT+AGG 0.323027 4_4:-32857346 Msa0678080:five_prime_UTR
AAACAATCATCTGCTATATC+AGG 0.347089 4_4:+32856241 None:intergenic
GCTGTAATATGTGAAGCATT+AGG 0.352990 4_4:+32855246 None:intergenic
AAAGCAGTCGAAGGTCTATC+TGG 0.356524 4_4:+32857021 None:intergenic
TTAGTTGATTGCTCTATGAT+TGG 0.367385 4_4:-32856151 Msa0678080:CDS
GCGCCTTCTCACAAGTATTC+AGG 0.382690 4_4:-32856362 Msa0678080:intron
CTGTAATATGTGAAGCATTA+GGG 0.386843 4_4:+32855247 None:intergenic
TCAAATTCTACTGAGGTTAC+CGG 0.399273 4_4:-32857227 Msa0678080:five_prime_UTR
GATGATAGGGTTTGTGAGAA+GGG 0.402280 4_4:+32857612 None:intergenic
TAGTTGATTGCTCTATGATT+GGG 0.404013 4_4:-32856150 Msa0678080:CDS
CAGATTTCCTGTTGATATCC+AGG 0.418869 4_4:-32855136 Msa0678080:intron
ATCAATACCTGGATATCAAC+AGG 0.418894 4_4:+32855129 None:intergenic
TTGCCACTAGAATAATTTGT+TGG 0.420265 4_4:-32854473 Msa0678080:three_prime_UTR
GGCTCACTGTCTATAAGTTA+AGG 0.428171 4_4:-32855905 Msa0678080:CDS
AATGTTCTTGTTTCTTTGGC+CGG 0.429116 4_4:-32856485 Msa0678080:CDS
ATCGGTGAGGGAAAAGAGAA+AGG 0.434137 4_4:+32857447 None:intergenic
CGAACTTCACCACAACCAAT+TGG 0.435988 4_4:+32856925 None:intergenic
TAGGAATAAATTTGTTATGC+TGG 0.438201 4_4:-32856821 Msa0678080:five_prime_UTR
TTTGTGATTATGTGGTGTTT+AGG 0.443685 4_4:-32856581 Msa0678080:five_prime_UTR
CAAGGCAGGGGCTTTAATGC+TGG 0.450060 4_4:-32857084 Msa0678080:intron
GCCTCAATCAAAACAAGATC+CGG 0.450430 4_4:+32856466 None:intergenic
GCAGCATCAAACATATCAAA+TGG 0.454928 4_4:+32855177 None:intergenic
AGAAGAAATTGTCAATTTGG+AGG 0.462443 4_4:+32857666 None:intergenic
TTTGGTTCTATGTGAGGCTA+TGG 0.465655 4_4:-32854561 Msa0678080:exon
GCTATGGTGTGAGATTTATA+TGG 0.465941 4_4:-32854545 Msa0678080:three_prime_UTR
GTTCTGAGAGAGGTTCAATA+AGG 0.468857 4_4:-32856851 Msa0678080:five_prime_UTR
AATATAGATTTGTGATTATG+TGG 0.471849 4_4:-32856589 Msa0678080:five_prime_UTR
AAGGTCTATCTGGATTGTTT+GGG 0.471918 4_4:+32857031 None:intergenic
TTCGCTTTCGTCTTCTGCAA+CGG 0.474504 4_4:+32857299 None:intergenic
ACGGTCGTTTCTCTTCATTT+CGG 0.476121 4_4:+32857318 None:intergenic
GGATAGGTGGGAATCAAGAT+TGG 0.482924 4_4:-32855220 Msa0678080:CDS
AGATGATAGGGTTTGTGAGA+AGG 0.486161 4_4:+32857611 None:intergenic
CTCAATCAAAACAGATATGA+AGG 0.488090 4_4:+32856405 None:intergenic
TGATCGGTTGAGAGATGAAA+GGG 0.489439 4_4:+32857566 None:intergenic
TTCGAGTAGGTTGGCAAAGA+CGG 0.491654 4_4:-32856708 Msa0678080:five_prime_UTR
AATCGGCGGCGGGAGGTGGT+TGG 0.491720 4_4:+32857485 None:intergenic
TTCAGTTTGGTGTAGGAGGA+CGG 0.496266 4_4:-32855286 Msa0678080:intron
TTTCATATCTAAGAGAGAAT+CGG 0.497576 4_4:+32857429 None:intergenic
TGAAGGATATAGTTGTGAAA+AGG 0.504325 4_4:+32856422 None:intergenic
GAGAGATGAAAGGGTTTGAG+AGG 0.505732 4_4:+32857575 None:intergenic
GGTTCAATAAGGAAAGTCAT+AGG 0.506604 4_4:-32856840 Msa0678080:five_prime_UTR
GAAGAAATTGTCAATTTGGA+GGG 0.508671 4_4:+32857667 None:intergenic
ATGATAGGGTTTGTGAGAAG+GGG 0.515062 4_4:+32857613 None:intergenic
TCGGTGAGGGAAAAGAGAAA+GGG 0.515165 4_4:+32857448 None:intergenic
ATCATCTGCTATATCAGGCC+GGG 0.517909 4_4:+32856246 None:intergenic
ACATATCAAATGGACGCCCT+TGG 0.519572 4_4:+32855187 None:intergenic
GAACAGAGAAAGAGAGGTCA+AGG 0.521699 4_4:+32857514 None:intergenic
AGGGTTTCAGTTTGAGAGAA+CGG 0.531395 4_4:+32857534 None:intergenic
GCAAAGACGGTGGATAAGAA+TGG 0.536821 4_4:-32856695 Msa0678080:five_prime_UTR
TGATGAACACAGACAGGGAC+TGG 0.540018 4_4:-32854518 Msa0678080:three_prime_UTR
TGAGAAGGCGCGTTGTGTGC+CGG 0.542492 4_4:+32856374 None:intergenic
TGATAGGGTTTGTGAGAAGG+GGG 0.545695 4_4:+32857614 None:intergenic
GGCACCATCTGCATGGAAAG+AGG 0.548701 4_4:-32855884 Msa0678080:CDS
TGCTTCACATATTACAGCAA+TGG 0.548985 4_4:-32855241 Msa0678080:CDS
AATCATCTGCTATATCAGGC+CGG 0.556123 4_4:+32856245 None:intergenic
GACTAAGAATGAATTACAAA+GGG 0.556790 4_4:+32857379 None:intergenic
GCACCATCTGCATGGAAAGA+GGG 0.557836 4_4:-32855883 Msa0678080:CDS
ATCAGAAGATAAAACTTCAC+CGG 0.561053 4_4:+32857208 None:intergenic
GGTGCGTGATGAACACAGAC+AGG 0.561080 4_4:-32854524 Msa0678080:three_prime_UTR
TCTTTGAATTTCGAGTAGGT+TGG 0.561301 4_4:-32856717 Msa0678080:five_prime_UTR
AGAACGGTGGAGAGGTTGAT+CGG 0.565965 4_4:+32857550 None:intergenic
TGCTGCAGGAATTCACAGCC+CGG 0.568216 4_4:-32856264 Msa0678080:intron
ATCAATTCGAGAGGAAGATA+CGG 0.569775 4_4:+32857253 None:intergenic
ATGCCCTCTTTCCATGCAGA+TGG 0.572500 4_4:+32855880 None:intergenic
TGTAGTGAGTGAGTGAGATA+GGG 0.575875 4_4:-32857345 Msa0678080:five_prime_UTR
ATCAACAGGAAATCTGTTGA+CGG 0.581442 4_4:+32855143 None:intergenic
TTTGGTCTGAGTTCTGAGAG+AGG 0.582217 4_4:-32856861 Msa0678080:five_prime_UTR
TATTACAGCAATGGATAGGT+GGG 0.584644 4_4:-32855232 Msa0678080:CDS
AGTCCAACAAATTATTCTAG+TGG 0.585826 4_4:+32854470 None:intergenic
TTGATCGGTTGAGAGATGAA+AGG 0.585978 4_4:+32857565 None:intergenic
CGACTAAGAATGAATTACAA+AGG 0.590314 4_4:+32857378 None:intergenic
TGAAAAGGCGCATTGTGTGC+CGG 0.591934 4_4:+32856437 None:intergenic
CTCACTCACTACAGCGAGAA+CGG 0.594898 4_4:+32857355 None:intergenic
ATATTACAGCAATGGATAGG+TGG 0.596045 4_4:-32855233 Msa0678080:CDS
ATCTGTGAACAGAGAAAGAG+AGG 0.604053 4_4:+32857508 None:intergenic
AAGTTAAGGCACCATCTGCA+TGG 0.608586 4_4:-32855891 Msa0678080:CDS
AAAACAAAGAAAGCAGTCGA+AGG 0.615661 4_4:+32857012 None:intergenic
ACACCTGAATACTTGTGAGA+AGG 0.616732 4_4:+32856359 None:intergenic
CACATATTACAGCAATGGAT+AGG 0.617205 4_4:-32855236 Msa0678080:CDS
TGGTGAAATGTTAAAACTGG+TGG 0.617548 4_4:-32854389 None:intergenic
TGATCATTCAAATTCTACTG+AGG 0.618347 4_4:-32857234 Msa0678080:five_prime_UTR
ATCTAAGAGAGAATCGGTGA+GGG 0.620102 4_4:+32857435 None:intergenic
GAGTAGGTTGGCAAAGACGG+TGG 0.628587 4_4:-32856705 Msa0678080:five_prime_UTR
TTGCAGAAGACGAAAGCGAA+CGG 0.630604 4_4:-32857296 Msa0678080:five_prime_UTR
TTGGGATCACAGTACAGCAT+AGG 0.632307 4_4:-32856132 Msa0678080:intron
AGTTTGAGAGAACGGTGGAG+AGG 0.644306 4_4:+32857542 None:intergenic
AACAGAGAAAGAGAGGTCAA+GGG 0.644341 4_4:+32857515 None:intergenic
GTTTCAGTTTGAGAGAACGG+TGG 0.655796 4_4:+32857537 None:intergenic
TCGAGAGGAAGATACGGCAG+CGG 0.657456 4_4:+32857259 None:intergenic
AGGACGGATGAACTTGTCGA+TGG 0.659217 4_4:-32855270 Msa0678080:CDS
ATTTGAATGATCAATTCGAG+AGG 0.661945 4_4:+32857244 None:intergenic
ATAGAGCAATCAACTAACGA+AGG 0.684184 4_4:+32856157 None:intergenic
TATCTAAGAGAGAATCGGTG+AGG 0.685839 4_4:+32857434 None:intergenic
GTGCGTGATGAACACAGACA+GGG 0.708064 4_4:-32854523 Msa0678080:three_prime_UTR
GGCGAGGGGATAAACAACTG+AGG 0.741182 4_4:+32856187 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTGATTTTTATTTATGT+TGG + chr4_4:32856669-32856688 None:intergenic 10.0%
!! AAAAATCAACAAAAATAAAC+AGG + chr4_4:32855176-32855195 None:intergenic 15.0%
!!! TGTTTATTTTTGTTGATTTT+TGG - chr4_4:32855175-32855194 Msa0678080:CDS 15.0%
!! ACTGTATGAAAAATAATTAT+GGG + chr4_4:32856076-32856095 None:intergenic 15.0%
!! AACTGTATGAAAAATAATTA+TGG + chr4_4:32856077-32856096 None:intergenic 15.0%
!! AATTCCAATTTAGTTATATT+GGG + chr4_4:32856567-32856586 None:intergenic 15.0%
!! AAATTCCAATTTAGTTATAT+TGG + chr4_4:32856568-32856587 None:intergenic 15.0%
!! TTTCATGTATATATCTAATT+TGG + chr4_4:32856963-32856982 None:intergenic 15.0%
!!! ATTCTATATTGTTTTTACTT+TGG - chr4_4:32857414-32857433 Msa0678080:five_prime_UTR 15.0%
!!! TTTATCTTTTACTAACCAAT+TGG - chr4_4:32855114-32855133 Msa0678080:intron 20.0%
!! AATGTTACTGTTCTTAATTA+TGG - chr4_4:32855271-32855290 Msa0678080:CDS 20.0%
!! AATATAGATTTGTGATTATG+TGG - chr4_4:32855465-32855484 Msa0678080:intron 20.0%
!!! CTGTTTTGATTTTAATTTAG+TGG - chr4_4:32855526-32855545 Msa0678080:intron 20.0%
!! TTATTTATTCTTGAGATATC+AGG - chr4_4:32856047-32856066 Msa0678080:intron 20.0%
!!! CTGCATTTTACTTTTATTTT+AGG - chr4_4:32856128-32856147 Msa0678080:intron 20.0%
!! AACATATATAGAGTTACTTA+AGG - chr4_4:32856220-32856239 Msa0678080:CDS 20.0%
!!! AAATAACTGGTATTTTAAGA+GGG + chr4_4:32856282-32856301 None:intergenic 20.0%
!!! AAAATAACTGGTATTTTAAG+AGG + chr4_4:32856283-32856302 None:intergenic 20.0%
!! TACTAAAAAGTAAAACAAAC+AGG + chr4_4:32856352-32856371 None:intergenic 20.0%
!! AAAACCCAATATAACTAAAT+TGG - chr4_4:32856560-32856579 Msa0678080:five_prime_UTR 20.0%
!!! TAAATATGTTTTTTCTCTCA+TGG - chr4_4:32857100-32857119 Msa0678080:intron 20.0%
!! AAAGAAACTAATTGTAAACA+TGG - chr4_4:32857145-32857164 Msa0678080:intron 20.0%
!! TTATTTCATCTAATTCTGTT+TGG - chr4_4:32857174-32857193 Msa0678080:intron 20.0%
!! AGACATAAATATTTAGTTGT+TGG - chr4_4:32857379-32857398 Msa0678080:five_prime_UTR 20.0%
!!! GTACTTGTAATTGTTATTTT+TGG - chr4_4:32857645-32857664 Msa0678080:five_prime_UTR 20.0%
!! TTTTAATAGACAAAAATCGG+CGG + chr4_4:32854586-32854605 None:intergenic 25.0%
!!! GGGTTTTAATAGACAAAAAT+CGG + chr4_4:32854589-32854608 None:intergenic 25.0%
! TTTCATATCTAAGAGAGAAT+CGG + chr4_4:32854628-32854647 None:intergenic 25.0%
! GACTAAGAATGAATTACAAA+GGG + chr4_4:32854678-32854697 None:intergenic 25.0%
!!! AGTTTTATCTTCTGATTGTA+AGG - chr4_4:32854853-32854872 Msa0678080:intron 25.0%
!! GTTTTATCTTCTGATTGTAA+GGG - chr4_4:32854854-32854873 Msa0678080:intron 25.0%
!!! GACTTTTTTTTTTACCTTCA+AGG - chr4_4:32854952-32854971 Msa0678080:intron 25.0%
!!! TAGGAATAAATTTGTTATGC+TGG - chr4_4:32855233-32855252 Msa0678080:CDS 25.0%
!!! ATTTTCTTTGAATTTCGAGT+AGG - chr4_4:32855333-32855352 Msa0678080:intron 25.0%
!! ATTGAATGTTCTTGTTTCTT+TGG - chr4_4:32855565-32855584 Msa0678080:intron 25.0%
! TCAAATACTATACATAGATC+TGG + chr4_4:32855752-32855771 None:intergenic 25.0%
!!! ATACCATTTTAGAATAGTGT+AGG + chr4_4:32855993-32856012 None:intergenic 25.0%
!! GTCTCTTGTTATAAAATAAC+TGG + chr4_4:32856295-32856314 None:intergenic 25.0%
!! TCAAAATTTTCAGCATTTTG+TGG - chr4_4:32856444-32856463 Msa0678080:CDS 25.0%
! AAAATGAACTTAGTACATGA+TGG + chr4_4:32856626-32856645 None:intergenic 25.0%
! GTATATTTGACATCTGATTA+GGG + chr4_4:32856692-32856711 None:intergenic 25.0%
! TGTATATTTGACATCTGATT+AGG + chr4_4:32856693-32856712 None:intergenic 25.0%
!!! CACTAATTTCTGAAATTTAG+AGG + chr4_4:32856731-32856750 None:intergenic 25.0%
! GAACAAAATTAATCAATACC+TGG + chr4_4:32856939-32856958 None:intergenic 25.0%
! GTAGAAAACAACATTAATGA+TGG + chr4_4:32856987-32857006 None:intergenic 25.0%
!!! AATGTTGTTTTCTACTTGAA+AGG - chr4_4:32856992-32857011 Msa0678080:five_prime_UTR 25.0%
! TCCACAAAATCATTATATGT+GGG + chr4_4:32857075-32857094 None:intergenic 25.0%
! TTCCACAAAATCATTATATG+TGG + chr4_4:32857076-32857095 None:intergenic 25.0%
!! TTTTGGTGAAATGTTAAAAC+TGG - chr4_4:32857662-32857681 Msa0678080:five_prime_UTR 25.0%
GAGAGAAGAAATTGTCAATT+TGG + chr4_4:32854394-32854413 None:intergenic 30.0%
!! TGATCTTTTGAGAGATGATA+GGG + chr4_4:32854458-32854477 None:intergenic 30.0%
!! TTGATCTTTTGAGAGATGAT+AGG + chr4_4:32854459-32854478 None:intergenic 30.0%
CGACTAAGAATGAATTACAA+AGG + chr4_4:32854679-32854698 None:intergenic 30.0%
ATTTGAATGATCAATTCGAG+AGG + chr4_4:32854813-32854832 None:intergenic 30.0%
TGATCATTCAAATTCTACTG+AGG - chr4_4:32854820-32854839 Msa0678080:intron 30.0%
ATCAGAAGATAAAACTTCAC+CGG + chr4_4:32854849-32854868 None:intergenic 30.0%
!!! TTTTTTTTTACCTTCAAGGC+AGG - chr4_4:32854956-32854975 Msa0678080:intron 30.0%
!!! TTTTTTTTACCTTCAAGGCA+GGG - chr4_4:32854957-32854976 Msa0678080:intron 30.0%
! TTTTACTAACCAATTGGTTG+TGG - chr4_4:32855120-32855139 Msa0678080:intron 30.0%
TTTGTGATTATGTGGTGTTT+AGG - chr4_4:32855473-32855492 Msa0678080:intron 30.0%
TGAAGGATATAGTTGTGAAA+AGG + chr4_4:32855635-32855654 None:intergenic 30.0%
CTCAATCAAAACAGATATGA+AGG + chr4_4:32855652-32855671 None:intergenic 30.0%
!!! CTTCATATCTGTTTTGATTG+AGG - chr4_4:32855650-32855669 Msa0678080:intron 30.0%
ATACTATACATAGATCTGGA+AGG + chr4_4:32855748-32855767 None:intergenic 30.0%
AAACAATCATCTGCTATATC+AGG + chr4_4:32855816-32855835 None:intergenic 30.0%
!!! AGCAGATGATTGTTTTTTGT+TGG - chr4_4:32855821-32855840 Msa0678080:intron 30.0%
!!! ATTGTTTTTTGTTGGCTTCA+AGG - chr4_4:32855829-32855848 Msa0678080:intron 30.0%
TTAGTTGATTGCTCTATGAT+TGG - chr4_4:32855903-32855922 Msa0678080:CDS 30.0%
TAGTTGATTGCTCTATGATT+GGG - chr4_4:32855904-32855923 Msa0678080:CDS 30.0%
! CATCCTACACTATTCTAAAA+TGG - chr4_4:32855987-32856006 Msa0678080:intron 30.0%
TAAAATGCAGACATTTATGC+AGG + chr4_4:32856118-32856137 None:intergenic 30.0%
AGTAAAACAAACAGGTTTGA+AGG + chr4_4:32856344-32856363 None:intergenic 30.0%
!!! TATATTGGGTTTTTTCTCTC+AGG + chr4_4:32856553-32856572 None:intergenic 30.0%
!!! ATACTACTGCTTTTTCAGTT+TGG - chr4_4:32856755-32856774 Msa0678080:five_prime_UTR 30.0%
! CTGTAATATGTGAAGCATTA+GGG + chr4_4:32856810-32856829 None:intergenic 30.0%
CCACAAAATCATTATATGTG+GGG + chr4_4:32857074-32857093 None:intergenic 30.0%
!!! AATAACAGCTCTTTTGGAAT+TGG + chr4_4:32857234-32857253 None:intergenic 30.0%
ATTCCAAAAGAGCTGTTATT+TGG - chr4_4:32857234-32857253 Msa0678080:five_prime_UTR 30.0%
! TAACCAAATAACAGCTCTTT+TGG + chr4_4:32857240-32857259 None:intergenic 30.0%
!! CTGATTTGTTCTAATTATGC+AGG - chr4_4:32857468-32857487 Msa0678080:five_prime_UTR 30.0%
! GTTCTAATTATGCAGGATTT+TGG - chr4_4:32857475-32857494 Msa0678080:five_prime_UTR 30.0%
! TTGCCACTAGAATAATTTGT+TGG - chr4_4:32857581-32857600 Msa0678080:five_prime_UTR 30.0%
AGTCCAACAAATTATTCTAG+TGG + chr4_4:32857587-32857606 None:intergenic 30.0%
ATCAATTCGAGAGGAAGATA+CGG + chr4_4:32854804-32854823 None:intergenic 35.0%
TCAAATTCTACTGAGGTTAC+CGG - chr4_4:32854827-32854846 Msa0678080:intron 35.0%
!!! TTTTTTTACCTTCAAGGCAG+GGG - chr4_4:32854958-32854977 Msa0678080:intron 35.0%
TCTGTCATAAAAAGCTGCTA+AGG - chr4_4:32854999-32855018 Msa0678080:intron 35.0%
AAGGTCTATCTGGATTGTTT+GGG + chr4_4:32855026-32855045 None:intergenic 35.0%
AAAACAAAGAAAGCAGTCGA+AGG + chr4_4:32855045-32855064 None:intergenic 35.0%
GGTTCAATAAGGAAAGTCAT+AGG - chr4_4:32855214-32855233 Msa0678080:CDS 35.0%
! TCTTTGAATTTCGAGTAGGT+TGG - chr4_4:32855337-32855356 Msa0678080:intron 35.0%
! AATGTTCTTGTTTCTTTGGC+CGG - chr4_4:32855569-32855588 Msa0678080:intron 35.0%
TCAACTAACGAAGGAAAAAC+TGG + chr4_4:32855891-32855910 None:intergenic 35.0%
ATAGAGCAATCAACTAACGA+AGG + chr4_4:32855900-32855919 None:intergenic 35.0%
TTGTGCAATTGTATGTGTGT+GGG + chr4_4:32855958-32855977 None:intergenic 35.0%
ATTGTGCAATTGTATGTGTG+TGG + chr4_4:32855959-32855978 None:intergenic 35.0%
AACTTGTAGCCAATTCGATT+TGG - chr4_4:32856588-32856607 Msa0678080:five_prime_UTR 35.0%
AGAGCATAACCAAATCGAAT+TGG + chr4_4:32856600-32856619 None:intergenic 35.0%
! GCTGTAATATGTGAAGCATT+AGG + chr4_4:32856811-32856830 None:intergenic 35.0%
TGCTTCACATATTACAGCAA+TGG - chr4_4:32856813-32856832 Msa0678080:five_prime_UTR 35.0%
CACATATTACAGCAATGGAT+AGG - chr4_4:32856818-32856837 Msa0678080:five_prime_UTR 35.0%
ATATTACAGCAATGGATAGG+TGG - chr4_4:32856821-32856840 Msa0678080:five_prime_UTR 35.0%
TATTACAGCAATGGATAGGT+GGG - chr4_4:32856822-32856841 Msa0678080:five_prime_UTR 35.0%
GCAGCATCAAACATATCAAA+TGG + chr4_4:32856880-32856899 None:intergenic 35.0%
ATCAACAGGAAATCTGTTGA+CGG + chr4_4:32856914-32856933 None:intergenic 35.0%
ATCAATACCTGGATATCAAC+AGG + chr4_4:32856928-32856947 None:intergenic 35.0%
! CCCCACATATAATGATTTTG+TGG - chr4_4:32857071-32857090 Msa0678080:five_prime_UTR 35.0%
ACTTCCAAAGCAGTTAAGAA+TGG + chr4_4:32857302-32857321 None:intergenic 35.0%
GCTATGGTGTGAGATTTATA+TGG - chr4_4:32857509-32857528 Msa0678080:five_prime_UTR 35.0%
ATGATAGGGTTTGTGAGAAG+GGG + chr4_4:32854444-32854463 None:intergenic 40.0%
GATGATAGGGTTTGTGAGAA+GGG + chr4_4:32854445-32854464 None:intergenic 40.0%
AGATGATAGGGTTTGTGAGA+AGG + chr4_4:32854446-32854465 None:intergenic 40.0%
TGATCGGTTGAGAGATGAAA+GGG + chr4_4:32854491-32854510 None:intergenic 40.0%
TTGATCGGTTGAGAGATGAA+AGG + chr4_4:32854492-32854511 None:intergenic 40.0%
AGGGTTTCAGTTTGAGAGAA+CGG + chr4_4:32854523-32854542 None:intergenic 40.0%
AACAGAGAAAGAGAGGTCAA+GGG + chr4_4:32854542-32854561 None:intergenic 40.0%
ATCTGTGAACAGAGAAAGAG+AGG + chr4_4:32854549-32854568 None:intergenic 40.0%
TAATAGACAAAAATCGGCGG+CGG + chr4_4:32854583-32854602 None:intergenic 40.0%
ATCTAAGAGAGAATCGGTGA+GGG + chr4_4:32854622-32854641 None:intergenic 40.0%
TATCTAAGAGAGAATCGGTG+AGG + chr4_4:32854623-32854642 None:intergenic 40.0%
TGTAGTGAGTGAGTGAGATA+GGG - chr4_4:32854709-32854728 Msa0678080:intron 40.0%
ACGGTCGTTTCTCTTCATTT+CGG + chr4_4:32854739-32854758 None:intergenic 40.0%
GAAGGTCTATCTGGATTGTT+TGG + chr4_4:32855027-32855046 None:intergenic 40.0%
!! GTTCTGAGAGAGGTTCAATA+AGG - chr4_4:32855203-32855222 Msa0678080:CDS 40.0%
GCCTCAATCAAAACAAGATC+CGG + chr4_4:32855591-32855610 None:intergenic 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG - chr4_4:32855598-32855617 Msa0678080:intron 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG - chr4_4:32855598-32855617 Msa0678080:intron 40.0%
ACACCTGAATACTTGTGAGA+AGG + chr4_4:32855698-32855717 None:intergenic 40.0%
CAAGTATTCAGGTGTGATTG+TGG - chr4_4:32855703-32855722 Msa0678080:intron 40.0%
!! CTTATCACACTTTTTGCTGC+AGG - chr4_4:32855776-32855795 Msa0678080:intron 40.0%
AATCATCTGCTATATCAGGC+CGG + chr4_4:32855812-32855831 None:intergenic 40.0%
ATCACAGTACAGCATAGGTA+TGG - chr4_4:32855927-32855946 Msa0678080:intron 40.0%
GGCTCACTGTCTATAAGTTA+AGG - chr4_4:32856149-32856168 Msa0678080:CDS 40.0%
CTATTTGGGCGATATATGGT+GGG + chr4_4:32856498-32856517 None:intergenic 40.0%
AGAGCTATTTGGGCGATATA+TGG + chr4_4:32856502-32856521 None:intergenic 40.0%
!!! CTGCTTTTTCAGTTTGGTGT+AGG - chr4_4:32856761-32856780 Msa0678080:five_prime_UTR 40.0%
!!! CTTTTTCAGTTTGGTGTAGG+AGG - chr4_4:32856764-32856783 Msa0678080:five_prime_UTR 40.0%
! AGATTGGACGAACTTTTCCA+AGG - chr4_4:32856850-32856869 Msa0678080:five_prime_UTR 40.0%
! GATTGGACGAACTTTTCCAA+GGG - chr4_4:32856851-32856870 Msa0678080:five_prime_UTR 40.0%
CAGATTTCCTGTTGATATCC+AGG - chr4_4:32856918-32856937 Msa0678080:five_prime_UTR 40.0%
TCTACTTGAAAGGCAACAAG+TGG - chr4_4:32857002-32857021 Msa0678080:five_prime_UTR 40.0%
GGCTAGAAGATAGCAAAGTT+AGG - chr4_4:32857121-32857140 Msa0678080:intron 40.0%
AAGGTTAGAAGCTACATGGA+AGG + chr4_4:32857278-32857297 None:intergenic 40.0%
CAAAGCAGTTAAGAATGGCA+AGG + chr4_4:32857297-32857316 None:intergenic 40.0%
CTTGCCATTCTTAACTGCTT+TGG - chr4_4:32857295-32857314 Msa0678080:five_prime_UTR 40.0%
! TTGTGCTATTGTCCTGAAGT+AGG + chr4_4:32857347-32857366 None:intergenic 40.0%
!!! CAGGATTTTGGTTCTATGTG+AGG - chr4_4:32857487-32857506 Msa0678080:five_prime_UTR 40.0%
!! TTTGGTTCTATGTGAGGCTA+TGG - chr4_4:32857493-32857512 Msa0678080:five_prime_UTR 40.0%
TGATAGGGTTTGTGAGAAGG+GGG + chr4_4:32854443-32854462 None:intergenic 45.0%
GAGAGATGAAAGGGTTTGAG+AGG + chr4_4:32854482-32854501 None:intergenic 45.0%
GTTTCAGTTTGAGAGAACGG+TGG + chr4_4:32854520-32854539 None:intergenic 45.0%
GAACAGAGAAAGAGAGGTCA+AGG + chr4_4:32854543-32854562 None:intergenic 45.0%
AATAGACAAAAATCGGCGGC+GGG + chr4_4:32854582-32854601 None:intergenic 45.0%
TCGGTGAGGGAAAAGAGAAA+GGG + chr4_4:32854609-32854628 None:intergenic 45.0%
ATCGGTGAGGGAAAAGAGAA+AGG + chr4_4:32854610-32854629 None:intergenic 45.0%
CTGTAGTGAGTGAGTGAGAT+AGG - chr4_4:32854708-32854727 Msa0678080:intron 45.0%
TTCGCTTTCGTCTTCTGCAA+CGG + chr4_4:32854758-32854777 None:intergenic 45.0%
TTGCAGAAGACGAAAGCGAA+CGG - chr4_4:32854758-32854777 Msa0678080:intron 45.0%
AAAGCAGTCGAAGGTCTATC+TGG + chr4_4:32855036-32855055 None:intergenic 45.0%
CGAACTTCACCACAACCAAT+TGG + chr4_4:32855132-32855151 None:intergenic 45.0%
!! TTTGGTCTGAGTTCTGAGAG+AGG - chr4_4:32855193-32855212 Msa0678080:CDS 45.0%
! TTCGAGTAGGTTGGCAAAGA+CGG - chr4_4:32855346-32855365 Msa0678080:intron 45.0%
GCAAAGACGGTGGATAAGAA+TGG - chr4_4:32855359-32855378 Msa0678080:intron 45.0%
!!! GCCGGATCTTGTTTTGATTG+AGG - chr4_4:32855587-32855606 Msa0678080:intron 45.0%
ATCATCTGCTATATCAGGCC+GGG + chr4_4:32855811-32855830 None:intergenic 45.0%
AACGAAGGAAAAACTGGCGA+GGG + chr4_4:32855885-32855904 None:intergenic 45.0%
TAACGAAGGAAAAACTGGCG+AGG + chr4_4:32855886-32855905 None:intergenic 45.0%
TTGGGATCACAGTACAGCAT+AGG - chr4_4:32855922-32855941 Msa0678080:intron 45.0%
! AAGTTAAGGCACCATCTGCA+TGG - chr4_4:32856163-32856182 Msa0678080:CDS 45.0%
!! GATATATGGTGGGCGATACA+TGG + chr4_4:32856488-32856507 None:intergenic 45.0%
GCTATTTGGGCGATATATGG+TGG + chr4_4:32856499-32856518 None:intergenic 45.0%
CGAAAGGCGAAGAGCTATTT+GGG + chr4_4:32856512-32856531 None:intergenic 45.0%
CCAAATAGCTCTTCGCCTTT+CGG - chr4_4:32856510-32856529 Msa0678080:five_prime_UTR 45.0%
CAAATAGCTCTTCGCCTTTC+GGG - chr4_4:32856511-32856530 Msa0678080:five_prime_UTR 45.0%
CTCTTCTAATGACTCCCGAA+AGG + chr4_4:32856528-32856547 None:intergenic 45.0%
!! TTCAGTTTGGTGTAGGAGGA+CGG - chr4_4:32856768-32856787 Msa0678080:five_prime_UTR 45.0%
GGATAGGTGGGAATCAAGAT+TGG - chr4_4:32856834-32856853 Msa0678080:five_prime_UTR 45.0%
ACATATCAAATGGACGCCCT+TGG + chr4_4:32856870-32856889 None:intergenic 45.0%
TGTGGGGACAAAAAGAACCT+AGG + chr4_4:32857058-32857077 None:intergenic 45.0%
TGGCAAGGTTAGAAGCTACA+TGG + chr4_4:32857282-32857301 None:intergenic 45.0%
GCTATTGTCCTGAAGTAGGT+AGG + chr4_4:32857343-32857362 None:intergenic 45.0%
! AGAACGGTGGAGAGGTTGAT+CGG + chr4_4:32854507-32854526 None:intergenic 50.0%
AGTTTGAGAGAACGGTGGAG+AGG + chr4_4:32854515-32854534 None:intergenic 50.0%
CTCACTCACTACAGCGAGAA+CGG + chr4_4:32854702-32854721 None:intergenic 50.0%
CATTAAAGCCCCTGCCTTGA+AGG + chr4_4:32854969-32854988 None:intergenic 50.0%
! TGAAAAGGCGCATTGTGTGC+CGG + chr4_4:32855620-32855639 None:intergenic 50.0%
GCGCCTTCTCACAAGTATTC+AGG - chr4_4:32855692-32855711 Msa0678080:intron 50.0%
ACGAAGGAAAAACTGGCGAG+GGG + chr4_4:32855884-32855903 None:intergenic 50.0%
TGCAGACATTTATGCAGGCG+AGG + chr4_4:32856113-32856132 None:intergenic 50.0%
GCACCATCTGCATGGAAAGA+GGG - chr4_4:32856171-32856190 Msa0678080:CDS 50.0%
ATGCCCTCTTTCCATGCAGA+TGG + chr4_4:32856177-32856196 None:intergenic 50.0%
! TGGTGGGCGATACATGGAAA+GGG + chr4_4:32856482-32856501 None:intergenic 50.0%
!! ATGGTGGGCGATACATGGAA+AGG + chr4_4:32856483-32856502 None:intergenic 50.0%
CCGAAAGGCGAAGAGCTATT+TGG + chr4_4:32856513-32856532 None:intergenic 50.0%
AGGACGGATGAACTTGTCGA+TGG - chr4_4:32856784-32856803 Msa0678080:five_prime_UTR 50.0%
! AAAGAACCTAGGCTGGTTGC+AGG + chr4_4:32857047-32857066 None:intergenic 50.0%
GGGACAAAAAGAACCTAGGC+TGG + chr4_4:32857054-32857073 None:intergenic 50.0%
CCTGCATGCTATAGCGAAGA+CGG + chr4_4:32857448-32857467 None:intergenic 50.0%
GTGCGTGATGAACACAGACA+GGG - chr4_4:32857531-32857550 Msa0678080:five_prime_UTR 50.0%
TGATGAACACAGACAGGGAC+TGG - chr4_4:32857536-32857555 Msa0678080:five_prime_UTR 50.0%
AAGGGGGAGTGAGAGTTGAG+GGG + chr4_4:32854427-32854446 None:intergenic 55.0%
GAAGGGGGAGTGAGAGTTGA+GGG + chr4_4:32854428-32854447 None:intergenic 55.0%
AGAAGGGGGAGTGAGAGTTG+AGG + chr4_4:32854429-32854448 None:intergenic 55.0%
TCGAGAGGAAGATACGGCAG+CGG + chr4_4:32854798-32854817 None:intergenic 55.0%
! CAAGGCAGGGGCTTTAATGC+TGG - chr4_4:32854970-32854989 Msa0678080:intron 55.0%
! GAGTAGGTTGGCAAAGACGG+TGG - chr4_4:32855349-32855368 Msa0678080:intron 55.0%
TGCTGCAGGAATTCACAGCC+CGG - chr4_4:32855790-32855809 Msa0678080:intron 55.0%
GGCGAGGGGATAAACAACTG+AGG + chr4_4:32855870-32855889 None:intergenic 55.0%
GCAGGCGAGGAAATCAGACA+TGG + chr4_4:32856100-32856119 None:intergenic 55.0%
GGCACCATCTGCATGGAAAG+AGG - chr4_4:32856170-32856189 Msa0678080:CDS 55.0%
GAACTTCCTGCAACCAGCCT+AGG - chr4_4:32857038-32857057 Msa0678080:five_prime_UTR 55.0%
CCTGAAGTAGGTAGGCACGT+TGG + chr4_4:32857335-32857354 None:intergenic 55.0%
CCAACGTGCCTACCTACTTC+AGG - chr4_4:32857332-32857351 Msa0678080:five_prime_UTR 55.0%
CCGTCTTCGCTATAGCATGC+AGG - chr4_4:32857445-32857464 Msa0678080:five_prime_UTR 55.0%
GGTGCGTGATGAACACAGAC+AGG - chr4_4:32857530-32857549 Msa0678080:five_prime_UTR 55.0%
AGACAAAAATCGGCGGCGGG+AGG + chr4_4:32854579-32854598 None:intergenic 60.0%
! TGAGAAGGCGCGTTGTGTGC+CGG + chr4_4:32855683-32855702 None:intergenic 60.0%
CAAAAATCGGCGGCGGGAGG+TGG + chr4_4:32854576-32854595 None:intergenic 65.0%
AATCGGCGGCGGGAGGTGGT+TGG + chr4_4:32854572-32854591 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 32854390 32857686 32854390 ID=Msa0678080;Name=Msa0678080
chr4_4 mRNA 32854390 32857686 32854390 ID=Msa0678080-mRNA-1;Parent=Msa0678080;Name=Msa0678080-mRNA-1;_AED=0.04;_eAED=0.04;_QI=1072|0.6|0.83|0.83|0.4|0.66|6|179|173
chr4_4 exon 32854390 32854586 32854390 ID=Msa0678080-mRNA-1:exon:8069;Parent=Msa0678080-mRNA-1
chr4_4 exon 32855137 32855303 32855137 ID=Msa0678080-mRNA-1:exon:8068;Parent=Msa0678080-mRNA-1
chr4_4 exon 32855880 32855926 32855880 ID=Msa0678080-mRNA-1:exon:8067;Parent=Msa0678080-mRNA-1
chr4_4 exon 32856133 32856278 32856133 ID=Msa0678080-mRNA-1:exon:8066;Parent=Msa0678080-mRNA-1
chr4_4 exon 32856363 32857098 32856363 ID=Msa0678080-mRNA-1:exon:8065;Parent=Msa0678080-mRNA-1
chr4_4 exon 32857207 32857686 32857207 ID=Msa0678080-mRNA-1:exon:8064;Parent=Msa0678080-mRNA-1
chr4_4 five_prime_UTR 32857207 32857686 32857207 ID=Msa0678080-mRNA-1:five_prime_utr;Parent=Msa0678080-mRNA-1
chr4_4 five_prime_UTR 32856507 32857098 32856507 ID=Msa0678080-mRNA-1:five_prime_utr;Parent=Msa0678080-mRNA-1
chr4_4 CDS 32856363 32856506 32856363 ID=Msa0678080-mRNA-1:cds;Parent=Msa0678080-mRNA-1
chr4_4 CDS 32856133 32856278 32856133 ID=Msa0678080-mRNA-1:cds;Parent=Msa0678080-mRNA-1
chr4_4 CDS 32855880 32855926 32855880 ID=Msa0678080-mRNA-1:cds;Parent=Msa0678080-mRNA-1
chr4_4 CDS 32855137 32855303 32855137 ID=Msa0678080-mRNA-1:cds;Parent=Msa0678080-mRNA-1
chr4_4 CDS 32854569 32854586 32854569 ID=Msa0678080-mRNA-1:cds;Parent=Msa0678080-mRNA-1
chr4_4 three_prime_UTR 32854390 32854568 32854390 ID=Msa0678080-mRNA-1:three_prime_utr;Parent=Msa0678080-mRNA-1
Gene Sequence

>Msa0678080

ATGTTCTTGTTTCTTTGGCCGGATCTTGTTTTGATTGAGGCGCTTTTCCGGCACACAATGCGCCTTTTCACAACTATATCCTTCATATCTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTCTCACAAGTATTCAGGAATTCACAGCCCGGCCTGATATAGCAGATGATTGTTTTTTGTTGGCTTCAAGGTGTATTCGCTACTGTCCTCAGTTGTTTATCCCCTCGCCAGTTTTTCCTTCGTTAGTTGATTGCTCTATGATTGGGATCACAGTACAGCATAGGCTCACTGTCTATAAGTTAAGGCACCATCTGCATGGAAAGAGGGCATTTTGGTGTAGGAGGACGGATGAACTTGTCGATGGCCCTAATGCTTCACATATTACAGCAATGGATAGGTGGGAATCAAGATTGGACGAACTTTTCCAAGGGCGTCCATTTGATATGTTTGATGCTGCTTTATCAGATACCGTCAACAGATTTCCTGTTGATATCCAGGATTTTGGTTCTATGTGA
Protein sequence

>Msa0678080

MFLFLWPDLVLIEALFRHTMRLFTTISFISVLIEALFRHTTRLLTSIQEFTARPDIADDCFLLASRCIRYCPQLFIPSPVFPSLVDCSMIGITVQHRLTVYKLRHHLHGKRAFWCRRTDELVDGPNASHITAMDRWESRLDELFQGRPFDMFDAALSDTVNRFPVDIQDFGSM*