AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1200940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1200940 MtrunA17_Chr4g0059761 83.333 78 13 0 28 105 740 817 2.58e-38 139
Msa1200940 MtrunA17_Chr5g0433601 42.775 173 76 5 11 175 83 240 1.42e-34 125
Msa1200940 MtrunA17_Chr3g0095661 72.581 62 17 0 114 175 138 199 1.94e-27 106
Msa1200940 MtrunA17_Chr3g0121311 61.290 62 24 0 114 175 127 188 1.46e-24 98.2
Msa1200940 MtrunA17_Chr5g0442671 67.742 62 20 0 114 175 131 192 3.40e-24 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1200940 AT5G62600.1 77.143 70 16 0 28 97 736 805 3.49e-32 121
Msa1200940 AT5G17230.3 75.806 62 15 0 114 175 182 243 8.54e-28 107
Msa1200940 AT5G17230.2 75.806 62 15 0 114 175 167 228 8.80e-28 107
Msa1200940 AT5G17230.1 75.806 62 15 0 114 175 167 228 8.80e-28 107
Msa1200940 AT5G17230.4 75.806 62 15 0 114 175 167 228 8.80e-28 107

Find 98 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTAATTATGCAGGATTT+TGG 0.187064 8_1:+54043782 Msa1200940:intron
GAGAGAAGAAATTGTCAATT+TGG 0.207441 8_1:-54040695 None:intergenic
ATTGAATGTTCTTGTTTCTT+TGG 0.213959 8_1:+54041867 Msa1200940:exon
GAAGGTCTATCTGGATTGTT+TGG 0.313321 8_1:-54041326 None:intergenic
CTGTAGTGAGTGAGTGAGAT+AGG 0.323027 8_1:+54041011 Msa1200940:five_prime_UTR
AAACAATCATCTGCTATATC+AGG 0.347089 8_1:-54042115 None:intergenic
AAAGCAGTCGAAGGTCTATC+TGG 0.356524 8_1:-54041335 None:intergenic
GCTGTAATATGTGAAGCATT+AGG 0.366470 8_1:-54043109 None:intergenic
TTAGTTGATTGCTCTATGAT+TGG 0.367385 8_1:+54042205 Msa1200940:CDS
GCGCCTTCTCACAAGTATTC+AGG 0.382690 8_1:+54041994 Msa1200940:CDS
CTGTAATATGTGAAGCATTA+GGG 0.396810 8_1:-54043108 None:intergenic
GATGAAAGGGTTTGTGAGAA+GGG 0.397542 8_1:-54040746 None:intergenic
TCAAATTCTACTGAGGTTAC+CGG 0.399273 8_1:+54041130 Msa1200940:five_prime_UTR
TAGTTGATTGCTCTATGATT+GGG 0.404013 8_1:+54042206 Msa1200940:CDS
CAGATTTCCTGTTGATATCC+AGG 0.418869 8_1:+54043225 Msa1200940:CDS
ATCAATACCTGGATATCAAC+AGG 0.418894 8_1:-54043232 None:intergenic
TTGCCACTAGAATAATTTGT+TGG 0.420265 8_1:+54043888 Msa1200940:three_prime_UTR
ATGAAAGGGTTTGTGAGAAG+GGG 0.425369 8_1:-54040745 None:intergenic
GGCTCACTGTCTATAAGTTA+AGG 0.428171 8_1:+54042451 Msa1200940:intron
AATGTTCTTGTTTCTTTGGC+CGG 0.429116 8_1:+54041871 Msa1200940:exon
ATCGGTGAGGGAAAAGAGAA+AGG 0.434137 8_1:-54040910 None:intergenic
CGAACTTCACCACAACCAAT+TGG 0.435988 8_1:-54041431 None:intergenic
TAGGAATAAATTTGTTATGC+TGG 0.438201 8_1:+54041535 Msa1200940:five_prime_UTR
TTTGTGATTATGTGGTGTTT+AGG 0.443685 8_1:+54041775 Msa1200940:five_prime_UTR
TTCGCTTTCGTCTTCTGCAA+TGG 0.448823 8_1:-54041058 None:intergenic
CAAGGCAGGGGCTTTAATGC+TGG 0.450060 8_1:+54041272 Msa1200940:intron
GCCTCAATCAAAACAAGATC+CGG 0.450430 8_1:-54041890 None:intergenic
GCAGCATCAAACATATCAAA+TGG 0.454928 8_1:-54043184 None:intergenic
ATGGTCGTTTCTCTTCATTT+CGG 0.456845 8_1:-54041039 None:intergenic
AGAAGAAATTGTCAATTTGG+AGG 0.462443 8_1:-54040692 None:intergenic
TTTGGTTCTATGTGAGGCTA+TGG 0.465655 8_1:+54043800 Msa1200940:exon
GCTATGGTGTGAGATTTATA+TGG 0.465941 8_1:+54043816 Msa1200940:three_prime_UTR
GTTCTGAGAGAGGTTCAATA+AGG 0.468857 8_1:+54041505 Msa1200940:five_prime_UTR
AATATAGATTTGTGATTATG+TGG 0.471849 8_1:+54041767 Msa1200940:five_prime_UTR
AAGGTCTATCTGGATTGTTT+GGG 0.471918 8_1:-54041325 None:intergenic
AGATGAAAGGGTTTGTGAGA+AGG 0.481423 8_1:-54040747 None:intergenic
GGATAGGTGGGAATCAAGAT+TGG 0.482924 8_1:+54043141 Msa1200940:CDS
CTCAATCAAAACAGATATGA+AGG 0.488090 8_1:-54041951 None:intergenic
TGATCTGTTGAGAGATGAAA+GGG 0.491251 8_1:-54040792 None:intergenic
TTCGAGTAGGTTGGCAAAGA+CGG 0.491654 8_1:+54041648 Msa1200940:five_prime_UTR
TTCAGTTTGGTGTAGGAGGA+CGG 0.496266 8_1:+54043069 Msa1200940:intron
TTTCATATCTAAGAGAGAAT+CGG 0.497576 8_1:-54040928 None:intergenic
TGAAGGATATAGTTGTGAAA+AGG 0.504325 8_1:-54041934 None:intergenic
GGTTCAATAAGGAAAGTCAT+AGG 0.506604 8_1:+54041516 Msa1200940:five_prime_UTR
GAAGAAATTGTCAATTTGGA+GGG 0.508671 8_1:-54040691 None:intergenic
TTGATCTGTTGAGAGATGAA+AGG 0.510142 8_1:-54040793 None:intergenic
TCGGTGAGGGAAAAGAGAAA+GGG 0.515165 8_1:-54040909 None:intergenic
ATCATCTGCTATATCAGGCC+GGG 0.517909 8_1:-54042110 None:intergenic
GAACAGAGAAAGAGAGGTCA+AGG 0.521699 8_1:-54040844 None:intergenic
AAATCGGCGGCAGAGGTGGT+TGG 0.523503 8_1:-54040873 None:intergenic
AGGGTTTCAGTTTGAGAGAA+CGG 0.531395 8_1:-54040824 None:intergenic
TGAAAGGGTTTGTGAGAAGG+GGG 0.534696 8_1:-54040744 None:intergenic
ACATATCAAATGGACACCCT+TGG 0.536490 8_1:-54043174 None:intergenic
GCAAAGACGGTGGATAAGAA+TGG 0.536821 8_1:+54041661 Msa1200940:five_prime_UTR
CAGCAACTGCAATGGATAGG+TGG 0.537534 8_1:+54043128 Msa1200940:CDS
TGATGAACACAGACAGGGAC+TGG 0.540018 8_1:+54043843 Msa1200940:three_prime_UTR
TGAGAAGGCGCGTTGTGTGC+CGG 0.542492 8_1:-54041982 None:intergenic
GGCACCATCTGCATGGAAAG+AGG 0.548701 8_1:+54042472 Msa1200940:CDS
AGCAACTGCAATGGATAGGT+GGG 0.555214 8_1:+54043129 Msa1200940:CDS
AATCATCTGCTATATCAGGC+CGG 0.556123 8_1:-54042111 None:intergenic
GACTAAGAATGAATTACAAA+GGG 0.556790 8_1:-54040978 None:intergenic
TGAAAAGGCGCGTTGTGTGC+CGG 0.557765 8_1:-54041919 None:intergenic
GCACCATCTGCATGGAAAGA+GGG 0.557836 8_1:+54042473 Msa1200940:CDS
ATCAGAAGATAAAACTTCAC+CGG 0.561053 8_1:-54041149 None:intergenic
GGTGCGTGATGAACACAGAC+AGG 0.561080 8_1:+54043837 Msa1200940:three_prime_UTR
TCTTTGAATTTCGAGTAGGT+TGG 0.561301 8_1:+54041639 Msa1200940:five_prime_UTR
TGCTGCAGGAATTCACAGCC+CGG 0.568216 8_1:+54042092 Msa1200940:intron
ATCAATTCGAGAGGAAGATA+CGG 0.569775 8_1:-54041104 None:intergenic
ATGCCCTCTTTCCATGCAGA+TGG 0.572500 8_1:-54042476 None:intergenic
TGTAGTGAGTGAGTGAGATA+GGG 0.575875 8_1:+54041012 Msa1200940:five_prime_UTR
GAGAGATGAAAGGGTTTGAG+AGG 0.578164 8_1:-54040783 None:intergenic
ATCAACAGGAAATCTGTTGA+CGG 0.581442 8_1:-54043218 None:intergenic
TTTGGTCTGAGTTCTGAGAG+AGG 0.582217 8_1:+54041495 Msa1200940:five_prime_UTR
AGTCCAACAAATTATTCTAG+TGG 0.585826 8_1:-54043891 None:intergenic
TTACAGCAACTGCAATGGAT+AGG 0.587244 8_1:+54043125 Msa1200940:CDS
ACATATTACAGCAACTGCAA+TGG 0.587371 8_1:+54043120 Msa1200940:CDS
CGACTAAGAATGAATTACAA+AGG 0.590314 8_1:-54040979 None:intergenic
CTCACTCACTACAGCGAGAA+CGG 0.594898 8_1:-54041002 None:intergenic
ATCTGTGAACAGAGAAAGAG+AGG 0.604053 8_1:-54040850 None:intergenic
AAGTTAAGGCACCATCTGCA+TGG 0.608586 8_1:+54042465 Msa1200940:CDS
AAAACAAAGAAAGCAGTCGA+AGG 0.615661 8_1:-54041344 None:intergenic
ACACCTGAATACTTGTGAGA+AGG 0.616732 8_1:-54041997 None:intergenic
TGGTGAAATGTTAAAACTGG+TGG 0.617548 8_1:+54043972 Msa1200940:three_prime_UTR
TGATCATTCAAATTCTACTG+AGG 0.618347 8_1:+54041123 Msa1200940:five_prime_UTR
ATCTAAGAGAGAATCGGTGA+GGG 0.620102 8_1:-54040922 None:intergenic
GAGTAGGTTGGCAAAGACGG+TGG 0.628587 8_1:+54041651 Msa1200940:five_prime_UTR
TTGCAGAAGACGAAAGCGAA+CGG 0.630194 8_1:+54041061 Msa1200940:five_prime_UTR
TTGGGATCACAGTACAGCAT+AGG 0.632307 8_1:+54042224 Msa1200940:CDS
AGTTTGAGAGAACGGTGGAG+AGG 0.644306 8_1:-54040816 None:intergenic
AACAGAGAAAGAGAGGTCAA+GGG 0.644341 8_1:-54040843 None:intergenic
GTTTCAGTTTGAGAGAACGG+TGG 0.655796 8_1:-54040821 None:intergenic
TCGAGAGGAAGATACGGCAG+CGG 0.657456 8_1:-54041098 None:intergenic
AGGACGGATGAACTTGTCGA+TGG 0.659217 8_1:+54043085 Msa1200940:CDS
ATTTGAATGATCAATTCGAG+AGG 0.661945 8_1:-54041113 None:intergenic
ATAGAGCAATCAACTAACGA+AGG 0.684184 8_1:-54042199 None:intergenic
TATCTAAGAGAGAATCGGTG+AGG 0.685839 8_1:-54040923 None:intergenic
GTGCGTGATGAACACAGACA+GGG 0.708064 8_1:+54043838 Msa1200940:three_prime_UTR
GGCGAGGGGATAAACAACTG+AGG 0.741182 8_1:-54042169 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTGATTTTTATTTATGT+TGG - chr8_1:54042970-54042989 None:intergenic 10.0%
!!! TGTTTATTTTTGTTGATTTT+TGG + chr8_1:54041477-54041496 Msa1200940:five_prime_UTR 15.0%
!!! AATGTTACTGTTTTTAATTA+TGG + chr8_1:54041573-54041592 Msa1200940:five_prime_UTR 15.0%
!! ACTCTATGAAAAATAATTAT+GGG - chr8_1:54042378-54042397 None:intergenic 15.0%
!! AACTCTATGAAAAATAATTA+TGG - chr8_1:54042379-54042398 None:intergenic 15.0%
!! TTTCATGTATATATCTAATT+TGG - chr8_1:54043270-54043289 None:intergenic 15.0%
!!! ATTCTATATTGTTTTTACTT+TGG + chr8_1:54043721-54043740 Msa1200940:intron 15.0%
!!! TTTATCTTTTACTAACCAAT+TGG + chr8_1:54041416-54041435 Msa1200940:five_prime_UTR 20.0%
!! AATATAGATTTGTGATTATG+TGG + chr8_1:54041767-54041786 Msa1200940:five_prime_UTR 20.0%
!!! CTGTTTTGATTTTAATTTAG+TGG + chr8_1:54041828-54041847 Msa1200940:five_prime_UTR 20.0%
!!! ATAACATTTTAGAATAGTGT+AGG - chr8_1:54042295-54042314 None:intergenic 20.0%
!! TTATTTATTCTTGAGATATC+AGG + chr8_1:54042349-54042368 Msa1200940:intron 20.0%
!!! CTGCATTTTACTTTTATTTT+AGG + chr8_1:54042430-54042449 Msa1200940:intron 20.0%
!! AACATATATAGAGTTACTTA+AGG + chr8_1:54042522-54042541 Msa1200940:intron 20.0%
!!! AAATAACTGGTATTTTAAGA+GGG - chr8_1:54042584-54042603 None:intergenic 20.0%
!!! AAAATAACTGGTATTTTAAG+AGG - chr8_1:54042585-54042604 None:intergenic 20.0%
!! TACTAAAAAGTAAAACAAAC+AGG - chr8_1:54042653-54042672 None:intergenic 20.0%
!! AAAACCCAATATAACTAAAT+TGG + chr8_1:54042861-54042880 Msa1200940:intron 20.0%
!! CATTCCAATTTAGTTATATT+GGG - chr8_1:54042868-54042887 None:intergenic 20.0%
!! ACATTCCAATTTAGTTATAT+TGG - chr8_1:54042869-54042888 None:intergenic 20.0%
!!! TAAATATGTTTTTTCTCTCA+TGG + chr8_1:54043407-54043426 Msa1200940:intron 20.0%
!! AAAGAAACTAATTGTAAACA+TGG + chr8_1:54043452-54043471 Msa1200940:intron 20.0%
!! TTATTTCATCTAATTCTGTT+TGG + chr8_1:54043481-54043500 Msa1200940:intron 20.0%
!! AGACATAAATATTTAGTTGT+TGG + chr8_1:54043686-54043705 Msa1200940:intron 20.0%
!!! GTACTTGTAATTGTTATTTT+TGG + chr8_1:54043952-54043971 Msa1200940:three_prime_UTR 20.0%
!! TTTTAATAGACAAAAATCGG+CGG - chr8_1:54040889-54040908 None:intergenic 25.0%
!!! GGGTTTTAATAGACAAAAAT+CGG - chr8_1:54040892-54040911 None:intergenic 25.0%
! TTTCATATCTAAGAGAGAAT+CGG - chr8_1:54040931-54040950 None:intergenic 25.0%
! GACTAAGAATGAATTACAAA+GGG - chr8_1:54040981-54041000 None:intergenic 25.0%
!!! AGTTTTATCTTCTGATTGTA+AGG + chr8_1:54041156-54041175 Msa1200940:intron 25.0%
!! GTTTTATCTTCTGATTGTAA+GGG + chr8_1:54041157-54041176 Msa1200940:intron 25.0%
!!! TGACTTTTTTTTTACCTTCA+AGG + chr8_1:54041254-54041273 Msa1200940:intron 25.0%
!!! TAGGAATAAATTTGTTATGC+TGG + chr8_1:54041535-54041554 Msa1200940:five_prime_UTR 25.0%
!!! ATTTTCTTTGAATTTCGAGT+AGG + chr8_1:54041635-54041654 Msa1200940:five_prime_UTR 25.0%
!! ATTGAATGTTCTTGTTTCTT+TGG + chr8_1:54041867-54041886 Msa1200940:exon 25.0%
! TCAAATACTATACATAGATC+TGG - chr8_1:54042054-54042073 None:intergenic 25.0%
!! GTCTCTTGTTATAAAATAAC+TGG - chr8_1:54042597-54042616 None:intergenic 25.0%
!! TCAAAATTTTCAGCATTTTG+TGG + chr8_1:54042745-54042764 Msa1200940:intron 25.0%
! AAAATGAACTTAGTACATGA+TGG - chr8_1:54042927-54042946 None:intergenic 25.0%
!!! CACTAATTTCTGAAATTTAG+AGG - chr8_1:54043032-54043051 None:intergenic 25.0%
! GAACAAAATTAATCAATACC+TGG - chr8_1:54043246-54043265 None:intergenic 25.0%
! GTAGAAAACAACATTAATGA+TGG - chr8_1:54043294-54043313 None:intergenic 25.0%
!!! CATTAATGTTGTTTTCTACT+TGG + chr8_1:54043295-54043314 Msa1200940:intron 25.0%
! TCCACAAAATCATTATATGT+GGG - chr8_1:54043382-54043401 None:intergenic 25.0%
! TTCCACAAAATCATTATATG+TGG - chr8_1:54043383-54043402 None:intergenic 25.0%
!! TTTTGGTGAAATGTTAAAAC+TGG + chr8_1:54043969-54043988 Msa1200940:three_prime_UTR 25.0%
GAGAGAAGAAATTGTCAATT+TGG - chr8_1:54040698-54040717 None:intergenic 30.0%
!! TGATCTTTTGAGAGATGAAA+GGG - chr8_1:54040762-54040781 None:intergenic 30.0%
!! TTGATCTTTTGAGAGATGAA+AGG - chr8_1:54040763-54040782 None:intergenic 30.0%
CGACTAAGAATGAATTACAA+AGG - chr8_1:54040982-54041001 None:intergenic 30.0%
ATTTGAATGATCAATTCGAG+AGG - chr8_1:54041116-54041135 None:intergenic 30.0%
TGATCATTCAAATTCTACTG+AGG + chr8_1:54041123-54041142 Msa1200940:five_prime_UTR 30.0%
ATCAGAAGATAAAACTTCAC+CGG - chr8_1:54041152-54041171 None:intergenic 30.0%
!!! TTTTTTTTTACCTTCAAGGC+AGG + chr8_1:54041258-54041277 Msa1200940:intron 30.0%
!!! TTTTTTTTACCTTCAAGGCA+GGG + chr8_1:54041259-54041278 Msa1200940:intron 30.0%
! TTTTACTAACCAATTGGTTG+TGG + chr8_1:54041422-54041441 Msa1200940:five_prime_UTR 30.0%
TTTGTGATTATGTGGTGTTT+AGG + chr8_1:54041775-54041794 Msa1200940:five_prime_UTR 30.0%
TGAAGGATATAGTTGTGAAA+AGG - chr8_1:54041937-54041956 None:intergenic 30.0%
CTCAATCAAAACAGATATGA+AGG - chr8_1:54041954-54041973 None:intergenic 30.0%
!!! CTTCATATCTGTTTTGATTG+AGG + chr8_1:54041952-54041971 Msa1200940:CDS 30.0%
ATACTATACATAGATCTGGA+AGG - chr8_1:54042050-54042069 None:intergenic 30.0%
AAACAATCATCTGCTATATC+AGG - chr8_1:54042118-54042137 None:intergenic 30.0%
!!! AGCAGATGATTGTTTTTTGT+TGG + chr8_1:54042123-54042142 Msa1200940:CDS 30.0%
!!! ATTGTTTTTTGTTGGCTTCA+AGG + chr8_1:54042131-54042150 Msa1200940:CDS 30.0%
TTAGTTGATTGCTCTATGAT+TGG + chr8_1:54042205-54042224 Msa1200940:CDS 30.0%
TAGTTGATTGCTCTATGATT+GGG + chr8_1:54042206-54042225 Msa1200940:CDS 30.0%
TAAAATGCAGACATTTATGC+AGG - chr8_1:54042420-54042439 None:intergenic 30.0%
AGTAAAACAAACAGGTTTGA+AGG - chr8_1:54042645-54042664 None:intergenic 30.0%
!!! TATATTGGGTTTTTTCTCTC+AGG - chr8_1:54042854-54042873 None:intergenic 30.0%
!!! ATACTACTGCTTTTTCAGTT+TGG + chr8_1:54043056-54043075 Msa1200940:intron 30.0%
! CTGTAATATGTGAAGCATTA+GGG - chr8_1:54043111-54043130 None:intergenic 30.0%
!!! AATGTTGTTTTCTACTTGGA+AGG + chr8_1:54043299-54043318 Msa1200940:intron 30.0%
CCACAAAATCATTATATGTG+GGG - chr8_1:54043381-54043400 None:intergenic 30.0%
!!! AATAACAGCTCTTTTGGAAT+TGG - chr8_1:54043541-54043560 None:intergenic 30.0%
ATTCCAAAAGAGCTGTTATT+TGG + chr8_1:54043541-54043560 Msa1200940:intron 30.0%
! TAACCAAATAACAGCTCTTT+TGG - chr8_1:54043547-54043566 None:intergenic 30.0%
!! CTGATTTGTTCTAATTATGC+AGG + chr8_1:54043775-54043794 Msa1200940:intron 30.0%
! GTTCTAATTATGCAGGATTT+TGG + chr8_1:54043782-54043801 Msa1200940:intron 30.0%
! TTGCCACTAGAATAATTTGT+TGG + chr8_1:54043888-54043907 Msa1200940:three_prime_UTR 30.0%
AGTCCAACAAATTATTCTAG+TGG - chr8_1:54043894-54043913 None:intergenic 30.0%
TGATCTGTTGAGAGATGAAA+GGG - chr8_1:54040795-54040814 None:intergenic 35.0%
TTGATCTGTTGAGAGATGAA+AGG - chr8_1:54040796-54040815 None:intergenic 35.0%
ATGGTCGTTTCTCTTCATTT+CGG - chr8_1:54041042-54041061 None:intergenic 35.0%
ATCAATTCGAGAGGAAGATA+CGG - chr8_1:54041107-54041126 None:intergenic 35.0%
TCAAATTCTACTGAGGTTAC+CGG + chr8_1:54041130-54041149 Msa1200940:five_prime_UTR 35.0%
!!! TTTTTTTACCTTCAAGGCAG+GGG + chr8_1:54041260-54041279 Msa1200940:intron 35.0%
TCTGTCATAAAAAGCTGCTA+AGG + chr8_1:54041301-54041320 Msa1200940:five_prime_UTR 35.0%
AAGGTCTATCTGGATTGTTT+GGG - chr8_1:54041328-54041347 None:intergenic 35.0%
AAAACAAAGAAAGCAGTCGA+AGG - chr8_1:54041347-54041366 None:intergenic 35.0%
GGTTCAATAAGGAAAGTCAT+AGG + chr8_1:54041516-54041535 Msa1200940:five_prime_UTR 35.0%
! TCTTTGAATTTCGAGTAGGT+TGG + chr8_1:54041639-54041658 Msa1200940:five_prime_UTR 35.0%
! AATGTTCTTGTTTCTTTGGC+CGG + chr8_1:54041871-54041890 Msa1200940:exon 35.0%
TCAACTAACGAAGGAAAAAC+TGG - chr8_1:54042193-54042212 None:intergenic 35.0%
ATAGAGCAATCAACTAACGA+AGG - chr8_1:54042202-54042221 None:intergenic 35.0%
TTGTGCAATTGTATGTGTGT+GGG - chr8_1:54042260-54042279 None:intergenic 35.0%
ATTGTGCAATTGTATGTGTG+TGG - chr8_1:54042261-54042280 None:intergenic 35.0%
AACTTGTAGCCAATTCGATT+TGG + chr8_1:54042889-54042908 Msa1200940:intron 35.0%
AGAGCATAACCAAATCGAAT+TGG - chr8_1:54042901-54042920 None:intergenic 35.0%
! GCTGTAATATGTGAAGCATT+AGG - chr8_1:54043112-54043131 None:intergenic 35.0%
ACATATTACAGCAACTGCAA+TGG + chr8_1:54043120-54043139 Msa1200940:CDS 35.0%
GCAGCATCAAACATATCAAA+TGG - chr8_1:54043187-54043206 None:intergenic 35.0%
ATCAACAGGAAATCTGTTGA+CGG - chr8_1:54043221-54043240 None:intergenic 35.0%
ATCAATACCTGGATATCAAC+AGG - chr8_1:54043235-54043254 None:intergenic 35.0%
! CCCCACATATAATGATTTTG+TGG + chr8_1:54043378-54043397 Msa1200940:intron 35.0%
ACTTCCAAAGCAGTTAAGAA+TGG - chr8_1:54043609-54043628 None:intergenic 35.0%
GCTATGGTGTGAGATTTATA+TGG + chr8_1:54043816-54043835 Msa1200940:three_prime_UTR 35.0%
ATGAAAGGGTTTGTGAGAAG+GGG - chr8_1:54040748-54040767 None:intergenic 40.0%
GATGAAAGGGTTTGTGAGAA+GGG - chr8_1:54040749-54040768 None:intergenic 40.0%
AGATGAAAGGGTTTGTGAGA+AGG - chr8_1:54040750-54040769 None:intergenic 40.0%
AGGGTTTCAGTTTGAGAGAA+CGG - chr8_1:54040827-54040846 None:intergenic 40.0%
AACAGAGAAAGAGAGGTCAA+GGG - chr8_1:54040846-54040865 None:intergenic 40.0%
ATCTGTGAACAGAGAAAGAG+AGG - chr8_1:54040853-54040872 None:intergenic 40.0%
ATCTAAGAGAGAATCGGTGA+GGG - chr8_1:54040925-54040944 None:intergenic 40.0%
TATCTAAGAGAGAATCGGTG+AGG - chr8_1:54040926-54040945 None:intergenic 40.0%
TGTAGTGAGTGAGTGAGATA+GGG + chr8_1:54041012-54041031 Msa1200940:five_prime_UTR 40.0%
GAAGGTCTATCTGGATTGTT+TGG - chr8_1:54041329-54041348 None:intergenic 40.0%
!! GTTCTGAGAGAGGTTCAATA+AGG + chr8_1:54041505-54041524 Msa1200940:five_prime_UTR 40.0%
GCCTCAATCAAAACAAGATC+CGG - chr8_1:54041893-54041912 None:intergenic 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr8_1:54041900-54041919 Msa1200940:CDS 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr8_1:54041900-54041919 Msa1200940:CDS 40.0%
ACACCTGAATACTTGTGAGA+AGG - chr8_1:54042000-54042019 None:intergenic 40.0%
CAAGTATTCAGGTGTGATTG+TGG + chr8_1:54042005-54042024 Msa1200940:intron 40.0%
!! CTTATCACACTTTTTGCTGC+AGG + chr8_1:54042078-54042097 Msa1200940:intron 40.0%
AATCATCTGCTATATCAGGC+CGG - chr8_1:54042114-54042133 None:intergenic 40.0%
ATCACAGTACAGCATAGGTA+TGG + chr8_1:54042229-54042248 Msa1200940:intron 40.0%
GGCTCACTGTCTATAAGTTA+AGG + chr8_1:54042451-54042470 Msa1200940:intron 40.0%
CTATTTGGGCGATATATGGT+GGG - chr8_1:54042799-54042818 None:intergenic 40.0%
AGAGCTATTTGGGCGATATA+TGG - chr8_1:54042803-54042822 None:intergenic 40.0%
CGAAAGGCAAAGAGCTATTT+GGG - chr8_1:54042813-54042832 None:intergenic 40.0%
CCAAATAGCTCTTTGCCTTT+CGG + chr8_1:54042811-54042830 Msa1200940:intron 40.0%
CAAATAGCTCTTTGCCTTTC+GGG + chr8_1:54042812-54042831 Msa1200940:intron 40.0%
!!! CTGCTTTTTCAGTTTGGTGT+AGG + chr8_1:54043062-54043081 Msa1200940:intron 40.0%
!!! CTTTTTCAGTTTGGTGTAGG+AGG + chr8_1:54043065-54043084 Msa1200940:intron 40.0%
TTACAGCAACTGCAATGGAT+AGG + chr8_1:54043125-54043144 Msa1200940:CDS 40.0%
! AGATTGGACGAACTTTTCCA+AGG + chr8_1:54043157-54043176 Msa1200940:CDS 40.0%
! GATTGGACGAACTTTTCCAA+GGG + chr8_1:54043158-54043177 Msa1200940:CDS 40.0%
ACATATCAAATGGACACCCT+TGG - chr8_1:54043177-54043196 None:intergenic 40.0%
CAGATTTCCTGTTGATATCC+AGG + chr8_1:54043225-54043244 Msa1200940:CDS 40.0%
GGCTAGAAGATAGCAAAGTT+AGG + chr8_1:54043428-54043447 Msa1200940:intron 40.0%
AAGGTTAGAAGCTACATGGA+AGG - chr8_1:54043585-54043604 None:intergenic 40.0%
CAAAGCAGTTAAGAATGGCA+AGG - chr8_1:54043604-54043623 None:intergenic 40.0%
CTTGCCATTCTTAACTGCTT+TGG + chr8_1:54043602-54043621 Msa1200940:intron 40.0%
! TTGTGCTATTGTCCTGAAGT+AGG - chr8_1:54043654-54043673 None:intergenic 40.0%
!!! CAGGATTTTGGTTCTATGTG+AGG + chr8_1:54043794-54043813 Msa1200940:intron 40.0%
!! TTTGGTTCTATGTGAGGCTA+TGG + chr8_1:54043800-54043819 Msa1200940:exon 40.0%
TGAAAGGGTTTGTGAGAAGG+GGG - chr8_1:54040747-54040766 None:intergenic 45.0%
GAGAGATGAAAGGGTTTGAG+AGG - chr8_1:54040786-54040805 None:intergenic 45.0%
GTTTCAGTTTGAGAGAACGG+TGG - chr8_1:54040824-54040843 None:intergenic 45.0%
GAACAGAGAAAGAGAGGTCA+AGG - chr8_1:54040847-54040866 None:intergenic 45.0%
TCGGTGAGGGAAAAGAGAAA+GGG - chr8_1:54040912-54040931 None:intergenic 45.0%
ATCGGTGAGGGAAAAGAGAA+AGG - chr8_1:54040913-54040932 None:intergenic 45.0%
CTGTAGTGAGTGAGTGAGAT+AGG + chr8_1:54041011-54041030 Msa1200940:five_prime_UTR 45.0%
TTCGCTTTCGTCTTCTGCAA+TGG - chr8_1:54041061-54041080 None:intergenic 45.0%
TTGCAGAAGACGAAAGCGAA+CGG + chr8_1:54041061-54041080 Msa1200940:five_prime_UTR 45.0%
AAAGCAGTCGAAGGTCTATC+TGG - chr8_1:54041338-54041357 None:intergenic 45.0%
CGAACTTCACCACAACCAAT+TGG - chr8_1:54041434-54041453 None:intergenic 45.0%
!! TTTGGTCTGAGTTCTGAGAG+AGG + chr8_1:54041495-54041514 Msa1200940:five_prime_UTR 45.0%
! TTCGAGTAGGTTGGCAAAGA+CGG + chr8_1:54041648-54041667 Msa1200940:five_prime_UTR 45.0%
GCAAAGACGGTGGATAAGAA+TGG + chr8_1:54041661-54041680 Msa1200940:five_prime_UTR 45.0%
!!! GCCGGATCTTGTTTTGATTG+AGG + chr8_1:54041889-54041908 Msa1200940:CDS 45.0%
ATCATCTGCTATATCAGGCC+GGG - chr8_1:54042113-54042132 None:intergenic 45.0%
AACGAAGGAAAAACTGGCGA+GGG - chr8_1:54042187-54042206 None:intergenic 45.0%
TAACGAAGGAAAAACTGGCG+AGG - chr8_1:54042188-54042207 None:intergenic 45.0%
TTGGGATCACAGTACAGCAT+AGG + chr8_1:54042224-54042243 Msa1200940:CDS 45.0%
! AAGTTAAGGCACCATCTGCA+TGG + chr8_1:54042465-54042484 Msa1200940:CDS 45.0%
!! GATATATGGTGGGCGATACA+TGG - chr8_1:54042789-54042808 None:intergenic 45.0%
GCTATTTGGGCGATATATGG+TGG - chr8_1:54042800-54042819 None:intergenic 45.0%
CCGAAAGGCAAAGAGCTATT+TGG - chr8_1:54042814-54042833 None:intergenic 45.0%
CTCTTCTAATGACTCCCGAA+AGG - chr8_1:54042829-54042848 None:intergenic 45.0%
!! TTCAGTTTGGTGTAGGAGGA+CGG + chr8_1:54043069-54043088 Msa1200940:intron 45.0%
AGCAACTGCAATGGATAGGT+GGG + chr8_1:54043129-54043148 Msa1200940:CDS 45.0%
GGATAGGTGGGAATCAAGAT+TGG + chr8_1:54043141-54043160 Msa1200940:CDS 45.0%
TGTGGGGACAAAAAGAACCT+AGG - chr8_1:54043365-54043384 None:intergenic 45.0%
TGGCAAGGTTAGAAGCTACA+TGG - chr8_1:54043589-54043608 None:intergenic 45.0%
GCTATTGTCCTGAAGTAGGT+AGG - chr8_1:54043650-54043669 None:intergenic 45.0%
AGTTTGAGAGAACGGTGGAG+AGG - chr8_1:54040819-54040838 None:intergenic 50.0%
TAGACAAAAATCGGCGGCAG+AGG - chr8_1:54040883-54040902 None:intergenic 50.0%
CTCACTCACTACAGCGAGAA+CGG - chr8_1:54041005-54041024 None:intergenic 50.0%
CATTAAAGCCCCTGCCTTGA+AGG - chr8_1:54041271-54041290 None:intergenic 50.0%
GCGCCTTCTCACAAGTATTC+AGG + chr8_1:54041994-54042013 Msa1200940:CDS 50.0%
ACGAAGGAAAAACTGGCGAG+GGG - chr8_1:54042186-54042205 None:intergenic 50.0%
TGCAGACATTTATGCAGGCG+AGG - chr8_1:54042415-54042434 None:intergenic 50.0%
GCACCATCTGCATGGAAAGA+GGG + chr8_1:54042473-54042492 Msa1200940:CDS 50.0%
ATGCCCTCTTTCCATGCAGA+TGG - chr8_1:54042479-54042498 None:intergenic 50.0%
! TGGTGGGCGATACATGGAAA+GGG - chr8_1:54042783-54042802 None:intergenic 50.0%
!! ATGGTGGGCGATACATGGAA+AGG - chr8_1:54042784-54042803 None:intergenic 50.0%
AGGACGGATGAACTTGTCGA+TGG + chr8_1:54043085-54043104 Msa1200940:CDS 50.0%
CAGCAACTGCAATGGATAGG+TGG + chr8_1:54043128-54043147 Msa1200940:CDS 50.0%
TCTACTTGGAAGGCAACGAG+TGG + chr8_1:54043309-54043328 Msa1200940:intron 50.0%
! AAAGAACCTAGGCTGGTTGC+AGG - chr8_1:54043354-54043373 None:intergenic 50.0%
GGGACAAAAAGAACCTAGGC+TGG - chr8_1:54043361-54043380 None:intergenic 50.0%
CCTGCATGCTATAGCGAAGA+CGG - chr8_1:54043755-54043774 None:intergenic 50.0%
GTGCGTGATGAACACAGACA+GGG + chr8_1:54043838-54043857 Msa1200940:three_prime_UTR 50.0%
TGATGAACACAGACAGGGAC+TGG + chr8_1:54043843-54043862 Msa1200940:three_prime_UTR 50.0%
AAGGGGGAGTGAGAGTTGAG+GGG - chr8_1:54040731-54040750 None:intergenic 55.0%
GAAGGGGGAGTGAGAGTTGA+GGG - chr8_1:54040732-54040751 None:intergenic 55.0%
AGAAGGGGGAGTGAGAGTTG+AGG - chr8_1:54040733-54040752 None:intergenic 55.0%
ACAAAAATCGGCGGCAGAGG+TGG - chr8_1:54040880-54040899 None:intergenic 55.0%
TCGAGAGGAAGATACGGCAG+CGG - chr8_1:54041101-54041120 None:intergenic 55.0%
! CAAGGCAGGGGCTTTAATGC+TGG + chr8_1:54041272-54041291 Msa1200940:intron 55.0%
! GAGTAGGTTGGCAAAGACGG+TGG + chr8_1:54041651-54041670 Msa1200940:five_prime_UTR 55.0%
! TGAAAAGGCGCGTTGTGTGC+CGG - chr8_1:54041922-54041941 None:intergenic 55.0%
TGCTGCAGGAATTCACAGCC+CGG + chr8_1:54042092-54042111 Msa1200940:intron 55.0%
GGCGAGGGGATAAACAACTG+AGG - chr8_1:54042172-54042191 None:intergenic 55.0%
GCAGGCGAGGAAATCAGACA+TGG - chr8_1:54042402-54042421 None:intergenic 55.0%
GGCACCATCTGCATGGAAAG+AGG + chr8_1:54042472-54042491 Msa1200940:CDS 55.0%
GAACTTCCTGCAACCAGCCT+AGG + chr8_1:54043345-54043364 Msa1200940:intron 55.0%
CCTGAAGTAGGTAGGCACGT+TGG - chr8_1:54043642-54043661 None:intergenic 55.0%
CCAACGTGCCTACCTACTTC+AGG + chr8_1:54043639-54043658 Msa1200940:intron 55.0%
CCGTCTTCGCTATAGCATGC+AGG + chr8_1:54043752-54043771 Msa1200940:intron 55.0%
GGTGCGTGATGAACACAGAC+AGG + chr8_1:54043837-54043856 Msa1200940:three_prime_UTR 55.0%
AAATCGGCGGCAGAGGTGGT+TGG - chr8_1:54040876-54040895 None:intergenic 60.0%
! TGAGAAGGCGCGTTGTGTGC+CGG - chr8_1:54041985-54042004 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 54040694 54043993 54040694 ID=Msa1200940;Name=Msa1200940
chr8_1 mRNA 54040694 54043993 54040694 ID=Msa1200940-mRNA-1;Parent=Msa1200940;Name=Msa1200940-mRNA-1;_AED=0.04;_eAED=0.04;_QI=1071|0.6|0.83|0.83|0.2|0.16|6|179|175
chr8_1 exon 54040694 54041172 54040694 ID=Msa1200940-mRNA-1:exon:17321;Parent=Msa1200940-mRNA-1
chr8_1 exon 54041280 54042015 54041280 ID=Msa1200940-mRNA-1:exon:17322;Parent=Msa1200940-mRNA-1
chr8_1 exon 54042100 54042245 54042100 ID=Msa1200940-mRNA-1:exon:17323;Parent=Msa1200940-mRNA-1
chr8_1 exon 54042452 54042498 54042452 ID=Msa1200940-mRNA-1:exon:17324;Parent=Msa1200940-mRNA-1
chr8_1 exon 54043074 54043246 54043074 ID=Msa1200940-mRNA-1:exon:17325;Parent=Msa1200940-mRNA-1
chr8_1 exon 54043797 54043993 54043797 ID=Msa1200940-mRNA-1:exon:17326;Parent=Msa1200940-mRNA-1
chr8_1 five_prime_UTR 54040694 54041172 54040694 ID=Msa1200940-mRNA-1:five_prime_utr;Parent=Msa1200940-mRNA-1
chr8_1 five_prime_UTR 54041280 54041871 54041280 ID=Msa1200940-mRNA-1:five_prime_utr;Parent=Msa1200940-mRNA-1
chr8_1 CDS 54041872 54042015 54041872 ID=Msa1200940-mRNA-1:cds;Parent=Msa1200940-mRNA-1
chr8_1 CDS 54042100 54042245 54042100 ID=Msa1200940-mRNA-1:cds;Parent=Msa1200940-mRNA-1
chr8_1 CDS 54042452 54042498 54042452 ID=Msa1200940-mRNA-1:cds;Parent=Msa1200940-mRNA-1
chr8_1 CDS 54043074 54043246 54043074 ID=Msa1200940-mRNA-1:cds;Parent=Msa1200940-mRNA-1
chr8_1 CDS 54043797 54043814 54043797 ID=Msa1200940-mRNA-1:cds;Parent=Msa1200940-mRNA-1
chr8_1 three_prime_UTR 54043815 54043993 54043815 ID=Msa1200940-mRNA-1:three_prime_utr;Parent=Msa1200940-mRNA-1
Gene Sequence

>Msa1200940

ATGTTCTTGTTTCTTTGGCCGGATCTTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTTTCACAACTATATCCTTCATATCTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTCTCACAAGTATTCAGGAATTCACAGCCCGGCCTGATATAGCAGATGATTGTTTTTTGTTGGCTTCAAGGTGTATTCGCTACTGTCCTCAGTTGTTTATCCCCTCGCCAGTTTTTCCTTCGTTAGTTGATTGCTCTATGATTGGGATCACAGTACAGCATAGGCTCACTGTCTATAAGTTAAGGCACCATCTGCATGGAAAGAGGGCATTTTGGTGTAGGAGGACGGATGAACTTGTCGATGGCCCTAATGCTTCACATATTACAGCAACTGCAATGGATAGGTGGGAATCAAGATTGGACGAACTTTTCCAAGGGTGTCCATTTGATATGTTTGATGCTGCTTTATCAGATACCGTCAACAGATTTCCTGTTGATATCCAGGATTTTGGTTCTATGTGA
Protein sequence

>Msa1200940

MFLFLWPDLVLIEALFRHTTRLFTTISFISVLIEALFRHTTRLLTSIQEFTARPDIADDCFLLASRCIRYCPQLFIPSPVFPSLVDCSMIGITVQHRLTVYKLRHHLHGKRAFWCRRTDELVDGPNASHITATAMDRWESRLDELFQGCPFDMFDAALSDTVNRFPVDIQDFGSM*