AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1136350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136350 MtrunA17_Chr4g0059761 83.333 78 13 0 28 105 740 817 2.39e-38 139
Msa1136350 MtrunA17_Chr5g0433601 43.353 173 75 5 11 175 83 240 7.60e-36 129
Msa1136350 MtrunA17_Chr3g0095661 74.194 62 16 0 114 175 138 199 1.41e-28 109
Msa1136350 MtrunA17_Chr5g0442671 69.355 62 19 0 114 175 131 192 1.91e-25 100
Msa1136350 MtrunA17_Chr3g0121311 59.677 62 25 0 114 175 127 188 1.10e-22 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136350 AT5G62600.1 77.143 70 16 0 28 97 736 805 2.37e-32 122
Msa1136350 AT5G17230.2 77.419 62 14 0 114 175 167 228 6.31e-29 110
Msa1136350 AT5G17230.1 77.419 62 14 0 114 175 167 228 6.31e-29 110
Msa1136350 AT5G17230.4 77.419 62 14 0 114 175 167 228 6.31e-29 110
Msa1136350 AT5G17230.3 77.419 62 14 0 114 175 182 243 6.58e-29 110

Find 101 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTAATTATGCAGGATTT+TGG 0.187064 7_4:+4739872 Msa1136350:intron
GAGAGAAGAAATTGTCAATT+TGG 0.207441 7_4:-4736783 None:intergenic
ATTGAATGTTCTTGTTTCTT+TGG 0.213959 7_4:+4737957 Msa1136350:exon
AATGTTACTGTTCTTAATTA+TGG 0.293181 7_4:+4737663 Msa1136350:five_prime_UTR
GAAGGTCTATCTGGATTGTT+TGG 0.313321 7_4:-4737416 None:intergenic
CTGTAGTGAGTGAGTGAGAT+AGG 0.323027 7_4:+4737100 Msa1136350:five_prime_UTR
AAACAATCATCTGCTATATC+AGG 0.347089 7_4:-4738205 None:intergenic
AAAGCAGTCGAAGGTCTATC+TGG 0.356524 7_4:-4737425 None:intergenic
GCTGTAATATGTGAAGCATT+AGG 0.366470 7_4:-4739199 None:intergenic
TTAGTTGATTGCTCTATGAT+TGG 0.367385 7_4:+4738295 Msa1136350:CDS
GCGCCTTCTCACAAGTATTC+AGG 0.382690 7_4:+4738084 Msa1136350:CDS
CTGTAATATGTGAAGCATTA+GGG 0.396810 7_4:-4739198 None:intergenic
GATGAAAGGGTTTGTGAGAA+GGG 0.397542 7_4:-4736834 None:intergenic
TCAAATTCTACTGAGGTTAC+CGG 0.399273 7_4:+4737219 Msa1136350:five_prime_UTR
TAGTTGATTGCTCTATGATT+GGG 0.404013 7_4:+4738296 Msa1136350:CDS
CAGATTTCCTGTTGATATCC+AGG 0.418869 7_4:+4739315 Msa1136350:CDS
ATCAATACCTGGATATCAAC+AGG 0.418894 7_4:-4739322 None:intergenic
TTGCCACTAGAATAATTTGT+TGG 0.420265 7_4:+4739978 Msa1136350:three_prime_UTR
ATGAAAGGGTTTGTGAGAAG+GGG 0.425369 7_4:-4736833 None:intergenic
GGCTCACTGTCTATAAGTTA+AGG 0.428171 7_4:+4738541 Msa1136350:intron
AATGTTCTTGTTTCTTTGGC+CGG 0.429116 7_4:+4737961 Msa1136350:exon
ATCGGTGAGGGAAAAGAGAA+AGG 0.434137 7_4:-4736999 None:intergenic
CGAACTTCACCACAACCAAT+TGG 0.435988 7_4:-4737521 None:intergenic
TAGGAATAAATTTGTTATGC+TGG 0.438201 7_4:+4737625 Msa1136350:five_prime_UTR
TTTGTGATTATGTGGTGTTT+AGG 0.443685 7_4:+4737865 Msa1136350:five_prime_UTR
CAAGGCAGGGGCTTTAATGC+TGG 0.450060 7_4:+4737362 Msa1136350:intron
GCCTCAATCAAAACAAGATC+CGG 0.450430 7_4:-4737980 None:intergenic
GCAGCATCAAACATATCAAA+TGG 0.454928 7_4:-4739274 None:intergenic
AGAAGAAATTGTCAATTTGG+AGG 0.462443 7_4:-4736780 None:intergenic
TTTGGTTCTATGTGAGGCTA+TGG 0.465655 7_4:+4739890 Msa1136350:exon
GCTATGGTGTGAGATTTATA+TGG 0.465941 7_4:+4739906 Msa1136350:three_prime_UTR
GTTCTGAGAGAGGTTCAATA+AGG 0.468857 7_4:+4737595 Msa1136350:five_prime_UTR
AATATAGATTTGTGATTATG+TGG 0.471849 7_4:+4737857 Msa1136350:five_prime_UTR
AAGGTCTATCTGGATTGTTT+GGG 0.471918 7_4:-4737415 None:intergenic
TTCGCTTTCGTCTTCTGCAA+CGG 0.474504 7_4:-4737147 None:intergenic
ACGGTCGTTTCTCTTCATTT+CGG 0.476121 7_4:-4737128 None:intergenic
GACGAAAGCGAACGGAGAAG+AGG 0.476736 7_4:+4737158 Msa1136350:five_prime_UTR
AGATGAAAGGGTTTGTGAGA+AGG 0.481423 7_4:-4736835 None:intergenic
GGATAGGTGGGAATCAAGAT+TGG 0.482924 7_4:+4739231 Msa1136350:CDS
CTCAATCAAAACAGATATGA+AGG 0.488090 7_4:-4738041 None:intergenic
TGATCGGTTGAGAGATGAAA+GGG 0.489439 7_4:-4736880 None:intergenic
TTCGAGTAGGTTGGCAAAGA+CGG 0.491654 7_4:+4737738 Msa1136350:five_prime_UTR
AATCGGCGGCGGGAGGTGGT+TGG 0.491720 7_4:-4736961 None:intergenic
TTCAGTTTGGTGTAGGAGGA+CGG 0.496266 7_4:+4739159 Msa1136350:intron
TTTCATATCTAAGAGAGAAT+CGG 0.497576 7_4:-4737017 None:intergenic
TGAAGGATATAGTTGTGAAA+AGG 0.504325 7_4:-4738024 None:intergenic
GAGAGATGAAAGGGTTTGAG+AGG 0.505732 7_4:-4736871 None:intergenic
GGTTCAATAAGGAAAGTCAT+AGG 0.506604 7_4:+4737606 Msa1136350:five_prime_UTR
GAAGAAATTGTCAATTTGGA+GGG 0.508671 7_4:-4736779 None:intergenic
TCGGTGAGGGAAAAGAGAAA+GGG 0.515165 7_4:-4736998 None:intergenic
ATCATCTGCTATATCAGGCC+GGG 0.517909 7_4:-4738200 None:intergenic
ACATATCAAATGGACGCCCT+TGG 0.519572 7_4:-4739264 None:intergenic
GAACAGAGAAAGAGAGGTCA+AGG 0.521699 7_4:-4736932 None:intergenic
AGGGTTTCAGTTTGAGAGAA+CGG 0.531395 7_4:-4736912 None:intergenic
TGAAAGGGTTTGTGAGAAGG+GGG 0.534696 7_4:-4736832 None:intergenic
GCAAAGACGGTGGATAAGAA+TGG 0.536821 7_4:+4737751 Msa1136350:five_prime_UTR
CAGCAACTGCAATGGATAGG+TGG 0.537534 7_4:+4739218 Msa1136350:CDS
TGATGAACACAGACAGGGAC+TGG 0.540018 7_4:+4739933 Msa1136350:three_prime_UTR
TGAGAAGGCGCGTTGTGTGC+CGG 0.542492 7_4:-4738072 None:intergenic
GGCACCATCTGCATGGAAAG+AGG 0.548701 7_4:+4738562 Msa1136350:CDS
AGCAACTGCAATGGATAGGT+GGG 0.555214 7_4:+4739219 Msa1136350:CDS
AATCATCTGCTATATCAGGC+CGG 0.556123 7_4:-4738201 None:intergenic
GACTAAGAATGAATTACAAA+GGG 0.556790 7_4:-4737067 None:intergenic
TGAAAAGGCGCGTTGTGTGC+CGG 0.557765 7_4:-4738009 None:intergenic
GCACCATCTGCATGGAAAGA+GGG 0.557836 7_4:+4738563 Msa1136350:CDS
ATCAGAAGATAAAACTTCAC+CGG 0.561053 7_4:-4737238 None:intergenic
GGTGCGTGATGAACACAGAC+AGG 0.561080 7_4:+4739927 Msa1136350:three_prime_UTR
TCTTTGAATTTCGAGTAGGT+TGG 0.561301 7_4:+4737729 Msa1136350:five_prime_UTR
AGAACGGTGGAGAGGTTGAT+CGG 0.565965 7_4:-4736896 None:intergenic
TGCTGCAGGAATTCACAGCC+CGG 0.568216 7_4:+4738182 Msa1136350:intron
ATCAATTCGAGAGGAAGATA+CGG 0.569775 7_4:-4737193 None:intergenic
ATGCCCTCTTTCCATGCAGA+TGG 0.572500 7_4:-4738566 None:intergenic
TGTAGTGAGTGAGTGAGATA+GGG 0.575875 7_4:+4737101 Msa1136350:five_prime_UTR
ATCAACAGGAAATCTGTTGA+CGG 0.581442 7_4:-4739308 None:intergenic
TTTGGTCTGAGTTCTGAGAG+AGG 0.582217 7_4:+4737585 Msa1136350:five_prime_UTR
AGTCCAACAAATTATTCTAG+TGG 0.585826 7_4:-4739981 None:intergenic
TTGATCGGTTGAGAGATGAA+AGG 0.585978 7_4:-4736881 None:intergenic
TTACAGCAACTGCAATGGAT+AGG 0.587244 7_4:+4739215 Msa1136350:CDS
ACATATTACAGCAACTGCAA+TGG 0.587371 7_4:+4739210 Msa1136350:CDS
CGACTAAGAATGAATTACAA+AGG 0.590314 7_4:-4737068 None:intergenic
CTCACTCACTACAGCGAGAA+CGG 0.594898 7_4:-4737091 None:intergenic
ATCTGTGAACAGAGAAAGAG+AGG 0.604053 7_4:-4736938 None:intergenic
AAGTTAAGGCACCATCTGCA+TGG 0.608586 7_4:+4738555 Msa1136350:CDS
AAAACAAAGAAAGCAGTCGA+AGG 0.615661 7_4:-4737434 None:intergenic
ACACCTGAATACTTGTGAGA+AGG 0.616732 7_4:-4738087 None:intergenic
TGGTGAAATGTTAAAACTGG+TGG 0.617548 7_4:+4740062 Msa1136350:three_prime_UTR
TGATCATTCAAATTCTACTG+AGG 0.618347 7_4:+4737212 Msa1136350:five_prime_UTR
ATCTAAGAGAGAATCGGTGA+GGG 0.620102 7_4:-4737011 None:intergenic
GAGTAGGTTGGCAAAGACGG+TGG 0.628587 7_4:+4737741 Msa1136350:five_prime_UTR
TTGCAGAAGACGAAAGCGAA+CGG 0.630604 7_4:+4737150 Msa1136350:five_prime_UTR
TTGGGATCACAGTACAGCAT+AGG 0.632307 7_4:+4738314 Msa1136350:CDS
AGTTTGAGAGAACGGTGGAG+AGG 0.644306 7_4:-4736904 None:intergenic
AACAGAGAAAGAGAGGTCAA+GGG 0.644341 7_4:-4736931 None:intergenic
GTTTCAGTTTGAGAGAACGG+TGG 0.655796 7_4:-4736909 None:intergenic
TCGAGAGGAAGATACGGCAG+CGG 0.657456 7_4:-4737187 None:intergenic
AGGACGGATGAACTTGTCGA+TGG 0.659217 7_4:+4739175 Msa1136350:CDS
ATTTGAATGATCAATTCGAG+AGG 0.661945 7_4:-4737202 None:intergenic
ATAGAGCAATCAACTAACGA+AGG 0.684184 7_4:-4738289 None:intergenic
TATCTAAGAGAGAATCGGTG+AGG 0.685839 7_4:-4737012 None:intergenic
GTGCGTGATGAACACAGACA+GGG 0.708064 7_4:+4739928 Msa1136350:three_prime_UTR
GGCGAGGGGATAAACAACTG+AGG 0.741182 7_4:-4738259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATATTGTTTTTACTT+TGG + chr7_4:4739811-4739830 Msa1136350:intron 10.0%
!!! TGTTTATTTTTGTTGATTTT+TGG + chr7_4:4737567-4737586 Msa1136350:five_prime_UTR 15.0%
!! ACTGTATGAAAAATAATTAT+GGG - chr7_4:4738468-4738487 None:intergenic 15.0%
!! AACTGTATGAAAAATAATTA+TGG - chr7_4:4738469-4738488 None:intergenic 15.0%
!!! AAATTGATTTTTGTTTATGT+TGG - chr7_4:4739060-4739079 None:intergenic 15.0%
!! TTTCATGTATATATCTAATT+TGG - chr7_4:4739360-4739379 None:intergenic 15.0%
!!! TTTATCTTTTACTAACCAAT+TGG + chr7_4:4737506-4737525 Msa1136350:five_prime_UTR 20.0%
!! AATGTTACTGTTCTTAATTA+TGG + chr7_4:4737663-4737682 Msa1136350:five_prime_UTR 20.0%
!! AATATAGATTTGTGATTATG+TGG + chr7_4:4737857-4737876 Msa1136350:five_prime_UTR 20.0%
!!! CTGTTTTGATTTTAATTTAG+TGG + chr7_4:4737918-4737937 Msa1136350:five_prime_UTR 20.0%
!! TTATTTATTCTTGAGATATC+AGG + chr7_4:4738439-4738458 Msa1136350:intron 20.0%
!!! CTGCATTTTACTTTTATTTT+AGG + chr7_4:4738520-4738539 Msa1136350:intron 20.0%
!! AACATATATAGAGTTACTTA+AGG + chr7_4:4738612-4738631 Msa1136350:intron 20.0%
!!! AAATAACTGGTATTTTAAGA+GGG - chr7_4:4738674-4738693 None:intergenic 20.0%
!!! AAAATAACTGGTATTTTAAG+AGG - chr7_4:4738675-4738694 None:intergenic 20.0%
!! TACTAAAAAGTAAAACAAAC+AGG - chr7_4:4738743-4738762 None:intergenic 20.0%
!! AAAACCCAATATAACTAAAT+TGG + chr7_4:4738951-4738970 Msa1136350:intron 20.0%
!! CATTCCAATTTAGTTATATT+GGG - chr7_4:4738958-4738977 None:intergenic 20.0%
!! ACATTCCAATTTAGTTATAT+TGG - chr7_4:4738959-4738978 None:intergenic 20.0%
!!! TAAATATGTTTTTTCTCTCA+TGG + chr7_4:4739497-4739516 Msa1136350:intron 20.0%
!! AAAGAAACTAATTGTAAACA+TGG + chr7_4:4739542-4739561 Msa1136350:intron 20.0%
!! TTATTTCATCTAATTCTGTT+TGG + chr7_4:4739571-4739590 Msa1136350:intron 20.0%
!! AGACATAAATATTTAGTTGT+TGG + chr7_4:4739776-4739795 Msa1136350:intron 20.0%
!!! GTACTTGTAATTGTTATTTT+TGG + chr7_4:4740042-4740061 Msa1136350:three_prime_UTR 20.0%
!! TTTTAATAGACAAAAATCGG+CGG - chr7_4:4736978-4736997 None:intergenic 25.0%
!!! GGGTTTTAATAGACAAAAAT+CGG - chr7_4:4736981-4737000 None:intergenic 25.0%
! TTTCATATCTAAGAGAGAAT+CGG - chr7_4:4737020-4737039 None:intergenic 25.0%
! GACTAAGAATGAATTACAAA+GGG - chr7_4:4737070-4737089 None:intergenic 25.0%
!!! AGTTTTATCTTCTGATTGTA+AGG + chr7_4:4737245-4737264 Msa1136350:intron 25.0%
!! GTTTTATCTTCTGATTGTAA+GGG + chr7_4:4737246-4737265 Msa1136350:intron 25.0%
!!! GACTTTTTTTTTTACCTTCA+AGG + chr7_4:4737344-4737363 Msa1136350:intron 25.0%
!!! TAGGAATAAATTTGTTATGC+TGG + chr7_4:4737625-4737644 Msa1136350:five_prime_UTR 25.0%
!!! ATTTTCTTTGAATTTCGAGT+AGG + chr7_4:4737725-4737744 Msa1136350:five_prime_UTR 25.0%
!! ATTGAATGTTCTTGTTTCTT+TGG + chr7_4:4737957-4737976 Msa1136350:exon 25.0%
! TCAAATACTATACATAGATC+TGG - chr7_4:4738144-4738163 None:intergenic 25.0%
!!! ATACCATTTTAGAATAGTGT+AGG - chr7_4:4738385-4738404 None:intergenic 25.0%
!! GTCTCTTGTTATAAAATAAC+TGG - chr7_4:4738687-4738706 None:intergenic 25.0%
!! TCAAAATTTTCAGCATTTTG+TGG + chr7_4:4738835-4738854 Msa1136350:intron 25.0%
!!! TATATTGGGTTTTTTCTCAA+AGG - chr7_4:4738944-4738963 None:intergenic 25.0%
! AAAATGAACTTAGTACATGA+TGG - chr7_4:4739017-4739036 None:intergenic 25.0%
! GTATATTTGACATCTGATTA+GGG - chr7_4:4739083-4739102 None:intergenic 25.0%
! TGTATATTTGACATCTGATT+AGG - chr7_4:4739084-4739103 None:intergenic 25.0%
!!! CACTAATTTCTGAAATTTAG+AGG - chr7_4:4739122-4739141 None:intergenic 25.0%
! GAACAAAATTAATCAATACC+TGG - chr7_4:4739336-4739355 None:intergenic 25.0%
! GTAGAAAACAACATTAATGA+TGG - chr7_4:4739384-4739403 None:intergenic 25.0%
!!! AATGTTGTTTTCTACTTGAA+AGG + chr7_4:4739389-4739408 Msa1136350:intron 25.0%
! TCCACAAAATCATTATATGT+GGG - chr7_4:4739472-4739491 None:intergenic 25.0%
! TTCCACAAAATCATTATATG+TGG - chr7_4:4739473-4739492 None:intergenic 25.0%
!! TTTTGGTGAAATGTTAAAAC+TGG + chr7_4:4740059-4740078 Msa1136350:three_prime_UTR 25.0%
GAGAGAAGAAATTGTCAATT+TGG - chr7_4:4736786-4736805 None:intergenic 30.0%
!! TGATCTTTTGAGAGATGAAA+GGG - chr7_4:4736850-4736869 None:intergenic 30.0%
!! TTGATCTTTTGAGAGATGAA+AGG - chr7_4:4736851-4736870 None:intergenic 30.0%
CGACTAAGAATGAATTACAA+AGG - chr7_4:4737071-4737090 None:intergenic 30.0%
ATTTGAATGATCAATTCGAG+AGG - chr7_4:4737205-4737224 None:intergenic 30.0%
TGATCATTCAAATTCTACTG+AGG + chr7_4:4737212-4737231 Msa1136350:five_prime_UTR 30.0%
ATCAGAAGATAAAACTTCAC+CGG - chr7_4:4737241-4737260 None:intergenic 30.0%
!!! TTTTTTTTTACCTTCAAGGC+AGG + chr7_4:4737348-4737367 Msa1136350:intron 30.0%
!!! TTTTTTTTACCTTCAAGGCA+GGG + chr7_4:4737349-4737368 Msa1136350:intron 30.0%
! TTTTACTAACCAATTGGTTG+TGG + chr7_4:4737512-4737531 Msa1136350:five_prime_UTR 30.0%
TTTGTGATTATGTGGTGTTT+AGG + chr7_4:4737865-4737884 Msa1136350:five_prime_UTR 30.0%
TGAAGGATATAGTTGTGAAA+AGG - chr7_4:4738027-4738046 None:intergenic 30.0%
CTCAATCAAAACAGATATGA+AGG - chr7_4:4738044-4738063 None:intergenic 30.0%
!!! CTTCATATCTGTTTTGATTG+AGG + chr7_4:4738042-4738061 Msa1136350:CDS 30.0%
ATACTATACATAGATCTGGA+AGG - chr7_4:4738140-4738159 None:intergenic 30.0%
AAACAATCATCTGCTATATC+AGG - chr7_4:4738208-4738227 None:intergenic 30.0%
!!! AGCAGATGATTGTTTTTTGT+TGG + chr7_4:4738213-4738232 Msa1136350:CDS 30.0%
!!! ATTGTTTTTTGTTGGCTTCA+AGG + chr7_4:4738221-4738240 Msa1136350:CDS 30.0%
TTAGTTGATTGCTCTATGAT+TGG + chr7_4:4738295-4738314 Msa1136350:CDS 30.0%
TAGTTGATTGCTCTATGATT+GGG + chr7_4:4738296-4738315 Msa1136350:CDS 30.0%
! CATCCTACACTATTCTAAAA+TGG + chr7_4:4738379-4738398 Msa1136350:intron 30.0%
TAAAATGCAGACATTTATGC+AGG - chr7_4:4738510-4738529 None:intergenic 30.0%
AGTAAAACAAACAGGTTTGA+AGG - chr7_4:4738735-4738754 None:intergenic 30.0%
!!! ATACTACTGCTTTTTCAGTT+TGG + chr7_4:4739146-4739165 Msa1136350:intron 30.0%
! CTGTAATATGTGAAGCATTA+GGG - chr7_4:4739201-4739220 None:intergenic 30.0%
CCACAAAATCATTATATGTG+GGG - chr7_4:4739471-4739490 None:intergenic 30.0%
!!! AATAACAGCTCTTTTGGAAT+TGG - chr7_4:4739631-4739650 None:intergenic 30.0%
ATTCCAAAAGAGCTGTTATT+TGG + chr7_4:4739631-4739650 Msa1136350:intron 30.0%
! TAACCAAATAACAGCTCTTT+TGG - chr7_4:4739637-4739656 None:intergenic 30.0%
!! CTGATTTGTTCTAATTATGC+AGG + chr7_4:4739865-4739884 Msa1136350:intron 30.0%
! GTTCTAATTATGCAGGATTT+TGG + chr7_4:4739872-4739891 Msa1136350:intron 30.0%
! TTGCCACTAGAATAATTTGT+TGG + chr7_4:4739978-4739997 Msa1136350:three_prime_UTR 30.0%
AGTCCAACAAATTATTCTAG+TGG - chr7_4:4739984-4740003 None:intergenic 30.0%
ATCAATTCGAGAGGAAGATA+CGG - chr7_4:4737196-4737215 None:intergenic 35.0%
TCAAATTCTACTGAGGTTAC+CGG + chr7_4:4737219-4737238 Msa1136350:five_prime_UTR 35.0%
!!! TTTTTTTACCTTCAAGGCAG+GGG + chr7_4:4737350-4737369 Msa1136350:intron 35.0%
TCTGTCATAAAAAGCTGCTA+AGG + chr7_4:4737391-4737410 Msa1136350:five_prime_UTR 35.0%
AAGGTCTATCTGGATTGTTT+GGG - chr7_4:4737418-4737437 None:intergenic 35.0%
AAAACAAAGAAAGCAGTCGA+AGG - chr7_4:4737437-4737456 None:intergenic 35.0%
GGTTCAATAAGGAAAGTCAT+AGG + chr7_4:4737606-4737625 Msa1136350:five_prime_UTR 35.0%
! TCTTTGAATTTCGAGTAGGT+TGG + chr7_4:4737729-4737748 Msa1136350:five_prime_UTR 35.0%
! AATGTTCTTGTTTCTTTGGC+CGG + chr7_4:4737961-4737980 Msa1136350:exon 35.0%
TCAACTAACGAAGGAAAAAC+TGG - chr7_4:4738283-4738302 None:intergenic 35.0%
ATAGAGCAATCAACTAACGA+AGG - chr7_4:4738292-4738311 None:intergenic 35.0%
TTGTGCAATTGTATGTGTGT+GGG - chr7_4:4738350-4738369 None:intergenic 35.0%
ATTGTGCAATTGTATGTGTG+TGG - chr7_4:4738351-4738370 None:intergenic 35.0%
AACTTGTAGCCAATTCGATT+TGG + chr7_4:4738979-4738998 Msa1136350:intron 35.0%
AGAGCATAACCAAATCGAAT+TGG - chr7_4:4738991-4739010 None:intergenic 35.0%
! GCTGTAATATGTGAAGCATT+AGG - chr7_4:4739202-4739221 None:intergenic 35.0%
ACATATTACAGCAACTGCAA+TGG + chr7_4:4739210-4739229 Msa1136350:CDS 35.0%
GCAGCATCAAACATATCAAA+TGG - chr7_4:4739277-4739296 None:intergenic 35.0%
ATCAACAGGAAATCTGTTGA+CGG - chr7_4:4739311-4739330 None:intergenic 35.0%
ATCAATACCTGGATATCAAC+AGG - chr7_4:4739325-4739344 None:intergenic 35.0%
! CCCCACATATAATGATTTTG+TGG + chr7_4:4739468-4739487 Msa1136350:intron 35.0%
ACTTCCAAAGCAGTTAAGAA+TGG - chr7_4:4739699-4739718 None:intergenic 35.0%
GCTATGGTGTGAGATTTATA+TGG + chr7_4:4739906-4739925 Msa1136350:three_prime_UTR 35.0%
ATGAAAGGGTTTGTGAGAAG+GGG - chr7_4:4736836-4736855 None:intergenic 40.0%
GATGAAAGGGTTTGTGAGAA+GGG - chr7_4:4736837-4736856 None:intergenic 40.0%
AGATGAAAGGGTTTGTGAGA+AGG - chr7_4:4736838-4736857 None:intergenic 40.0%
TGATCGGTTGAGAGATGAAA+GGG - chr7_4:4736883-4736902 None:intergenic 40.0%
TTGATCGGTTGAGAGATGAA+AGG - chr7_4:4736884-4736903 None:intergenic 40.0%
AGGGTTTCAGTTTGAGAGAA+CGG - chr7_4:4736915-4736934 None:intergenic 40.0%
AACAGAGAAAGAGAGGTCAA+GGG - chr7_4:4736934-4736953 None:intergenic 40.0%
ATCTGTGAACAGAGAAAGAG+AGG - chr7_4:4736941-4736960 None:intergenic 40.0%
TAATAGACAAAAATCGGCGG+CGG - chr7_4:4736975-4736994 None:intergenic 40.0%
ATCTAAGAGAGAATCGGTGA+GGG - chr7_4:4737014-4737033 None:intergenic 40.0%
TATCTAAGAGAGAATCGGTG+AGG - chr7_4:4737015-4737034 None:intergenic 40.0%
TGTAGTGAGTGAGTGAGATA+GGG + chr7_4:4737101-4737120 Msa1136350:five_prime_UTR 40.0%
ACGGTCGTTTCTCTTCATTT+CGG - chr7_4:4737131-4737150 None:intergenic 40.0%
GAAGGTCTATCTGGATTGTT+TGG - chr7_4:4737419-4737438 None:intergenic 40.0%
!! GTTCTGAGAGAGGTTCAATA+AGG + chr7_4:4737595-4737614 Msa1136350:five_prime_UTR 40.0%
GCCTCAATCAAAACAAGATC+CGG - chr7_4:4737983-4738002 None:intergenic 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr7_4:4737990-4738009 Msa1136350:CDS 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr7_4:4737990-4738009 Msa1136350:CDS 40.0%
ACACCTGAATACTTGTGAGA+AGG - chr7_4:4738090-4738109 None:intergenic 40.0%
CAAGTATTCAGGTGTGATTG+TGG + chr7_4:4738095-4738114 Msa1136350:intron 40.0%
!! CTTATCACACTTTTTGCTGC+AGG + chr7_4:4738168-4738187 Msa1136350:intron 40.0%
AATCATCTGCTATATCAGGC+CGG - chr7_4:4738204-4738223 None:intergenic 40.0%
ATCACAGTACAGCATAGGTA+TGG + chr7_4:4738319-4738338 Msa1136350:intron 40.0%
GGCTCACTGTCTATAAGTTA+AGG + chr7_4:4738541-4738560 Msa1136350:intron 40.0%
CTATTTGGGCGATATATGGT+GGG - chr7_4:4738889-4738908 None:intergenic 40.0%
AGAGCTATTTGGGCGATATA+TGG - chr7_4:4738893-4738912 None:intergenic 40.0%
!!! CTGCTTTTTCAGTTTGGTGT+AGG + chr7_4:4739152-4739171 Msa1136350:intron 40.0%
!!! CTTTTTCAGTTTGGTGTAGG+AGG + chr7_4:4739155-4739174 Msa1136350:intron 40.0%
TTACAGCAACTGCAATGGAT+AGG + chr7_4:4739215-4739234 Msa1136350:CDS 40.0%
! AGATTGGACGAACTTTTCCA+AGG + chr7_4:4739247-4739266 Msa1136350:CDS 40.0%
! GATTGGACGAACTTTTCCAA+GGG + chr7_4:4739248-4739267 Msa1136350:CDS 40.0%
CAGATTTCCTGTTGATATCC+AGG + chr7_4:4739315-4739334 Msa1136350:CDS 40.0%
GGCTAGAAGATAGCAAAGTT+AGG + chr7_4:4739518-4739537 Msa1136350:intron 40.0%
AAGGTTAGAAGCTACATGGA+AGG - chr7_4:4739675-4739694 None:intergenic 40.0%
CAAAGCAGTTAAGAATGGCA+AGG - chr7_4:4739694-4739713 None:intergenic 40.0%
CTTGCCATTCTTAACTGCTT+TGG + chr7_4:4739692-4739711 Msa1136350:intron 40.0%
! TTGTGCTATTGTCCTGAAGT+AGG - chr7_4:4739744-4739763 None:intergenic 40.0%
!!! CAGGATTTTGGTTCTATGTG+AGG + chr7_4:4739884-4739903 Msa1136350:intron 40.0%
!! TTTGGTTCTATGTGAGGCTA+TGG + chr7_4:4739890-4739909 Msa1136350:exon 40.0%
TGAAAGGGTTTGTGAGAAGG+GGG - chr7_4:4736835-4736854 None:intergenic 45.0%
GAGAGATGAAAGGGTTTGAG+AGG - chr7_4:4736874-4736893 None:intergenic 45.0%
GTTTCAGTTTGAGAGAACGG+TGG - chr7_4:4736912-4736931 None:intergenic 45.0%
GAACAGAGAAAGAGAGGTCA+AGG - chr7_4:4736935-4736954 None:intergenic 45.0%
AATAGACAAAAATCGGCGGC+GGG - chr7_4:4736974-4736993 None:intergenic 45.0%
TCGGTGAGGGAAAAGAGAAA+GGG - chr7_4:4737001-4737020 None:intergenic 45.0%
ATCGGTGAGGGAAAAGAGAA+AGG - chr7_4:4737002-4737021 None:intergenic 45.0%
CTGTAGTGAGTGAGTGAGAT+AGG + chr7_4:4737100-4737119 Msa1136350:five_prime_UTR 45.0%
TTCGCTTTCGTCTTCTGCAA+CGG - chr7_4:4737150-4737169 None:intergenic 45.0%
TTGCAGAAGACGAAAGCGAA+CGG + chr7_4:4737150-4737169 Msa1136350:five_prime_UTR 45.0%
AAAGCAGTCGAAGGTCTATC+TGG - chr7_4:4737428-4737447 None:intergenic 45.0%
CGAACTTCACCACAACCAAT+TGG - chr7_4:4737524-4737543 None:intergenic 45.0%
!! TTTGGTCTGAGTTCTGAGAG+AGG + chr7_4:4737585-4737604 Msa1136350:five_prime_UTR 45.0%
! TTCGAGTAGGTTGGCAAAGA+CGG + chr7_4:4737738-4737757 Msa1136350:five_prime_UTR 45.0%
GCAAAGACGGTGGATAAGAA+TGG + chr7_4:4737751-4737770 Msa1136350:five_prime_UTR 45.0%
!!! GCCGGATCTTGTTTTGATTG+AGG + chr7_4:4737979-4737998 Msa1136350:CDS 45.0%
ATCATCTGCTATATCAGGCC+GGG - chr7_4:4738203-4738222 None:intergenic 45.0%
AACGAAGGAAAAACTGGCGA+GGG - chr7_4:4738277-4738296 None:intergenic 45.0%
TAACGAAGGAAAAACTGGCG+AGG - chr7_4:4738278-4738297 None:intergenic 45.0%
TTGGGATCACAGTACAGCAT+AGG + chr7_4:4738314-4738333 Msa1136350:CDS 45.0%
! AAGTTAAGGCACCATCTGCA+TGG + chr7_4:4738555-4738574 Msa1136350:CDS 45.0%
!! GATATATGGTGGGCGATACA+TGG - chr7_4:4738879-4738898 None:intergenic 45.0%
GCTATTTGGGCGATATATGG+TGG - chr7_4:4738890-4738909 None:intergenic 45.0%
CGAAAGGCGAAGAGCTATTT+GGG - chr7_4:4738903-4738922 None:intergenic 45.0%
CCAAATAGCTCTTCGCCTTT+CGG + chr7_4:4738901-4738920 Msa1136350:intron 45.0%
CAAATAGCTCTTCGCCTTTC+GGG + chr7_4:4738902-4738921 Msa1136350:intron 45.0%
CTCTTCTAATGACTCCCGAA+AGG - chr7_4:4738919-4738938 None:intergenic 45.0%
!! TTCAGTTTGGTGTAGGAGGA+CGG + chr7_4:4739159-4739178 Msa1136350:intron 45.0%
AGCAACTGCAATGGATAGGT+GGG + chr7_4:4739219-4739238 Msa1136350:CDS 45.0%
GGATAGGTGGGAATCAAGAT+TGG + chr7_4:4739231-4739250 Msa1136350:CDS 45.0%
ACATATCAAATGGACGCCCT+TGG - chr7_4:4739267-4739286 None:intergenic 45.0%
TCTACTTGAAAGGCAACGAG+TGG + chr7_4:4739399-4739418 Msa1136350:intron 45.0%
TGTGGGGACAAAAAGAACCT+AGG - chr7_4:4739455-4739474 None:intergenic 45.0%
TGGCAAGGTTAGAAGCTACA+TGG - chr7_4:4739679-4739698 None:intergenic 45.0%
GCTATTGTCCTGAAGTAGGT+AGG - chr7_4:4739740-4739759 None:intergenic 45.0%
! AGAACGGTGGAGAGGTTGAT+CGG - chr7_4:4736899-4736918 None:intergenic 50.0%
AGTTTGAGAGAACGGTGGAG+AGG - chr7_4:4736907-4736926 None:intergenic 50.0%
CTCACTCACTACAGCGAGAA+CGG - chr7_4:4737094-4737113 None:intergenic 50.0%
CATTAAAGCCCCTGCCTTGA+AGG - chr7_4:4737361-4737380 None:intergenic 50.0%
GCGCCTTCTCACAAGTATTC+AGG + chr7_4:4738084-4738103 Msa1136350:CDS 50.0%
ACGAAGGAAAAACTGGCGAG+GGG - chr7_4:4738276-4738295 None:intergenic 50.0%
TGCAGACATTTATGCAGGCG+AGG - chr7_4:4738505-4738524 None:intergenic 50.0%
GCACCATCTGCATGGAAAGA+GGG + chr7_4:4738563-4738582 Msa1136350:CDS 50.0%
ATGCCCTCTTTCCATGCAGA+TGG - chr7_4:4738569-4738588 None:intergenic 50.0%
! TGGTGGGCGATACATGGAAA+GGG - chr7_4:4738873-4738892 None:intergenic 50.0%
!! ATGGTGGGCGATACATGGAA+AGG - chr7_4:4738874-4738893 None:intergenic 50.0%
CCGAAAGGCGAAGAGCTATT+TGG - chr7_4:4738904-4738923 None:intergenic 50.0%
AGGACGGATGAACTTGTCGA+TGG + chr7_4:4739175-4739194 Msa1136350:CDS 50.0%
CAGCAACTGCAATGGATAGG+TGG + chr7_4:4739218-4739237 Msa1136350:CDS 50.0%
! AAAGAACCTAGGCTGGTTGC+AGG - chr7_4:4739444-4739463 None:intergenic 50.0%
GGGACAAAAAGAACCTAGGC+TGG - chr7_4:4739451-4739470 None:intergenic 50.0%
CCTGCATGCTATAGCGAAGA+CGG - chr7_4:4739845-4739864 None:intergenic 50.0%
GTGCGTGATGAACACAGACA+GGG + chr7_4:4739928-4739947 Msa1136350:three_prime_UTR 50.0%
TGATGAACACAGACAGGGAC+TGG + chr7_4:4739933-4739952 Msa1136350:three_prime_UTR 50.0%
AAGGGGGAGTGAGAGTTGAG+GGG - chr7_4:4736819-4736838 None:intergenic 55.0%
GAAGGGGGAGTGAGAGTTGA+GGG - chr7_4:4736820-4736839 None:intergenic 55.0%
AGAAGGGGGAGTGAGAGTTG+AGG - chr7_4:4736821-4736840 None:intergenic 55.0%
GACGAAAGCGAACGGAGAAG+AGG + chr7_4:4737158-4737177 Msa1136350:five_prime_UTR 55.0%
TCGAGAGGAAGATACGGCAG+CGG - chr7_4:4737190-4737209 None:intergenic 55.0%
! CAAGGCAGGGGCTTTAATGC+TGG + chr7_4:4737362-4737381 Msa1136350:intron 55.0%
! GAGTAGGTTGGCAAAGACGG+TGG + chr7_4:4737741-4737760 Msa1136350:five_prime_UTR 55.0%
! TGAAAAGGCGCGTTGTGTGC+CGG - chr7_4:4738012-4738031 None:intergenic 55.0%
TGCTGCAGGAATTCACAGCC+CGG + chr7_4:4738182-4738201 Msa1136350:intron 55.0%
GGCGAGGGGATAAACAACTG+AGG - chr7_4:4738262-4738281 None:intergenic 55.0%
GCAGGCGAGGAAATCAGACA+TGG - chr7_4:4738492-4738511 None:intergenic 55.0%
GGCACCATCTGCATGGAAAG+AGG + chr7_4:4738562-4738581 Msa1136350:CDS 55.0%
GAACTTCCTGCAACCAGCCT+AGG + chr7_4:4739435-4739454 Msa1136350:intron 55.0%
CCTGAAGTAGGTAGGCACGT+TGG - chr7_4:4739732-4739751 None:intergenic 55.0%
CCAACGTGCCTACCTACTTC+AGG + chr7_4:4739729-4739748 Msa1136350:intron 55.0%
CCGTCTTCGCTATAGCATGC+AGG + chr7_4:4739842-4739861 Msa1136350:intron 55.0%
GGTGCGTGATGAACACAGAC+AGG + chr7_4:4739927-4739946 Msa1136350:three_prime_UTR 55.0%
AGACAAAAATCGGCGGCGGG+AGG - chr7_4:4736971-4736990 None:intergenic 60.0%
! TGAGAAGGCGCGTTGTGTGC+CGG - chr7_4:4738075-4738094 None:intergenic 60.0%
CAAAAATCGGCGGCGGGAGG+TGG - chr7_4:4736968-4736987 None:intergenic 65.0%
AATCGGCGGCGGGAGGTGGT+TGG - chr7_4:4736964-4736983 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 4736782 4740083 4736782 ID=Msa1136350;Name=Msa1136350
chr7_4 mRNA 4736782 4740083 4736782 ID=Msa1136350-mRNA-1;Parent=Msa1136350;Name=Msa1136350-mRNA-1;_AED=0.04;_eAED=0.04;_QI=1072|0.6|0.83|0.83|0.4|0.33|6|179|175
chr7_4 exon 4736782 4737261 4736782 ID=Msa1136350-mRNA-1:exon:1254;Parent=Msa1136350-mRNA-1
chr7_4 exon 4737370 4738105 4737370 ID=Msa1136350-mRNA-1:exon:1255;Parent=Msa1136350-mRNA-1
chr7_4 exon 4738190 4738335 4738190 ID=Msa1136350-mRNA-1:exon:1256;Parent=Msa1136350-mRNA-1
chr7_4 exon 4738542 4738588 4738542 ID=Msa1136350-mRNA-1:exon:1257;Parent=Msa1136350-mRNA-1
chr7_4 exon 4739164 4739336 4739164 ID=Msa1136350-mRNA-1:exon:1258;Parent=Msa1136350-mRNA-1
chr7_4 exon 4739887 4740083 4739887 ID=Msa1136350-mRNA-1:exon:1259;Parent=Msa1136350-mRNA-1
chr7_4 five_prime_UTR 4736782 4737261 4736782 ID=Msa1136350-mRNA-1:five_prime_utr;Parent=Msa1136350-mRNA-1
chr7_4 five_prime_UTR 4737370 4737961 4737370 ID=Msa1136350-mRNA-1:five_prime_utr;Parent=Msa1136350-mRNA-1
chr7_4 CDS 4737962 4738105 4737962 ID=Msa1136350-mRNA-1:cds;Parent=Msa1136350-mRNA-1
chr7_4 CDS 4738190 4738335 4738190 ID=Msa1136350-mRNA-1:cds;Parent=Msa1136350-mRNA-1
chr7_4 CDS 4738542 4738588 4738542 ID=Msa1136350-mRNA-1:cds;Parent=Msa1136350-mRNA-1
chr7_4 CDS 4739164 4739336 4739164 ID=Msa1136350-mRNA-1:cds;Parent=Msa1136350-mRNA-1
chr7_4 CDS 4739887 4739904 4739887 ID=Msa1136350-mRNA-1:cds;Parent=Msa1136350-mRNA-1
chr7_4 three_prime_UTR 4739905 4740083 4739905 ID=Msa1136350-mRNA-1:three_prime_utr;Parent=Msa1136350-mRNA-1
Gene Sequence

>Msa1136350

ATGTTCTTGTTTCTTTGGCCGGATCTTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTTTCACAACTATATCCTTCATATCTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTCTCACAAGTATTCAGGAATTCACAGCCCGGCCTGATATAGCAGATGATTGTTTTTTGTTGGCTTCAAGGTGTATTCGCTACTGTCCTCAGTTGTTTATCCCCTCGCCAGTTTTTCCTTCGTTAGTTGATTGCTCTATGATTGGGATCACAGTACAGCATAGGCTCACTGTCTATAAGTTAAGGCACCATCTGCATGGAAAGAGGGCATTTTGGTGTAGGAGGACGGATGAACTTGTCGATGGCCCTAATGCTTCACATATTACAGCAACTGCAATGGATAGGTGGGAATCAAGATTGGACGAACTTTTCCAAGGGCGTCCATTTGATATGTTTGATGCTGCTTTATCAGATACCGTCAACAGATTTCCTGTTGATATCCAGGATTTTGGTTCTATGTGA
Protein sequence

>Msa1136350

MFLFLWPDLVLIEALFRHTTRLFTTISFISVLIEALFRHTTRLLTSIQEFTARPDIADDCFLLASRCIRYCPQLFIPSPVFPSLVDCSMIGITVQHRLTVYKLRHHLHGKRAFWCRRTDELVDGPNASHITATAMDRWESRLDELFQGRPFDMFDAALSDTVNRFPVDIQDFGSM*