AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0717560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0717560 MtrunA17_Chr4g0059761 83.333 78 13 0 28 105 740 817 2.58e-38 139
Msa0717560 MtrunA17_Chr5g0433601 42.775 173 76 5 11 175 83 240 1.42e-34 125
Msa0717560 MtrunA17_Chr3g0095661 72.581 62 17 0 114 175 138 199 1.94e-27 106
Msa0717560 MtrunA17_Chr3g0121311 61.290 62 24 0 114 175 127 188 1.46e-24 98.2
Msa0717560 MtrunA17_Chr5g0442671 67.742 62 20 0 114 175 131 192 3.40e-24 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0717560 AT5G62600.1 77.143 70 16 0 28 97 736 805 3.49e-32 121
Msa0717560 AT5G17230.3 75.806 62 15 0 114 175 182 243 8.54e-28 107
Msa0717560 AT5G17230.2 75.806 62 15 0 114 175 167 228 8.80e-28 107
Msa0717560 AT5G17230.1 75.806 62 15 0 114 175 167 228 8.80e-28 107
Msa0717560 AT5G17230.4 75.806 62 15 0 114 175 167 228 8.80e-28 107

Find 98 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTAATTATGCAGGATTT+TGG 0.187064 5_1:+11446779 Msa0717560:intron
GAGAGAAGAAATTGTCAATT+TGG 0.207441 5_1:-11443692 None:intergenic
ATTGAATGTTCTTGTTTCTT+TGG 0.213959 5_1:+11444864 Msa0717560:exon
GAAGGTCTATCTGGATTGTT+TGG 0.313321 5_1:-11444323 None:intergenic
CTGTAGTGAGTGAGTGAGAT+AGG 0.323027 5_1:+11444008 Msa0717560:five_prime_UTR
AAACAATCATCTGCTATATC+AGG 0.347089 5_1:-11445112 None:intergenic
AAAGCAGTCGAAGGTCTATC+TGG 0.356524 5_1:-11444332 None:intergenic
GCTGTAATATGTGAAGCATT+AGG 0.366470 5_1:-11446106 None:intergenic
TTAGTTGATTGCTCTATGAT+TGG 0.367385 5_1:+11445202 Msa0717560:CDS
GCGCCTTCTCACAAGTATTC+AGG 0.382690 5_1:+11444991 Msa0717560:CDS
CTGTAATATGTGAAGCATTA+GGG 0.396810 5_1:-11446105 None:intergenic
GATGAAAGGGTTTGTGAGAA+GGG 0.397542 5_1:-11443743 None:intergenic
TCAAATTCTACTGAGGTTAC+CGG 0.399273 5_1:+11444127 Msa0717560:five_prime_UTR
TAGTTGATTGCTCTATGATT+GGG 0.404013 5_1:+11445203 Msa0717560:CDS
CAGATTTCCTGTTGATATCC+AGG 0.418869 5_1:+11446222 Msa0717560:CDS
ATCAATACCTGGATATCAAC+AGG 0.418894 5_1:-11446229 None:intergenic
TTGCCACTAGAATAATTTGT+TGG 0.420265 5_1:+11446885 Msa0717560:three_prime_UTR
ATGAAAGGGTTTGTGAGAAG+GGG 0.425369 5_1:-11443742 None:intergenic
GGCTCACTGTCTATAAGTTA+AGG 0.428171 5_1:+11445448 Msa0717560:intron
AATGTTCTTGTTTCTTTGGC+CGG 0.429116 5_1:+11444868 Msa0717560:exon
ATCGGTGAGGGAAAAGAGAA+AGG 0.434137 5_1:-11443907 None:intergenic
CGAACTTCACCACAACCAAT+TGG 0.435988 5_1:-11444428 None:intergenic
TAGGAATAAATTTGTTATGC+TGG 0.438201 5_1:+11444532 Msa0717560:five_prime_UTR
TTTGTGATTATGTGGTGTTT+AGG 0.443685 5_1:+11444772 Msa0717560:five_prime_UTR
TTCGCTTTCGTCTTCTGCAA+TGG 0.448823 5_1:-11444055 None:intergenic
CAAGGCAGGGGCTTTAATGC+TGG 0.450060 5_1:+11444269 Msa0717560:intron
GCCTCAATCAAAACAAGATC+CGG 0.450430 5_1:-11444887 None:intergenic
GCAGCATCAAACATATCAAA+TGG 0.454928 5_1:-11446181 None:intergenic
ATGGTCGTTTCTCTTCATTT+CGG 0.456845 5_1:-11444036 None:intergenic
AGAAGAAATTGTCAATTTGG+AGG 0.462443 5_1:-11443689 None:intergenic
TTTGGTTCTATGTGAGGCTA+TGG 0.465655 5_1:+11446797 Msa0717560:exon
GCTATGGTGTGAGATTTATA+TGG 0.465941 5_1:+11446813 Msa0717560:three_prime_UTR
GTTCTGAGAGAGGTTCAATA+AGG 0.468857 5_1:+11444502 Msa0717560:five_prime_UTR
AATATAGATTTGTGATTATG+TGG 0.471849 5_1:+11444764 Msa0717560:five_prime_UTR
AAGGTCTATCTGGATTGTTT+GGG 0.471918 5_1:-11444322 None:intergenic
AGATGAAAGGGTTTGTGAGA+AGG 0.481423 5_1:-11443744 None:intergenic
GGATAGGTGGGAATCAAGAT+TGG 0.482924 5_1:+11446138 Msa0717560:CDS
CTCAATCAAAACAGATATGA+AGG 0.488090 5_1:-11444948 None:intergenic
TGATCTGTTGAGAGATGAAA+GGG 0.491251 5_1:-11443789 None:intergenic
TTCGAGTAGGTTGGCAAAGA+CGG 0.491654 5_1:+11444645 Msa0717560:five_prime_UTR
TTCAGTTTGGTGTAGGAGGA+CGG 0.496266 5_1:+11446066 Msa0717560:intron
TTTCATATCTAAGAGAGAAT+CGG 0.497576 5_1:-11443925 None:intergenic
TGAAGGATATAGTTGTGAAA+AGG 0.504325 5_1:-11444931 None:intergenic
GGTTCAATAAGGAAAGTCAT+AGG 0.506604 5_1:+11444513 Msa0717560:five_prime_UTR
GAAGAAATTGTCAATTTGGA+GGG 0.508671 5_1:-11443688 None:intergenic
TTGATCTGTTGAGAGATGAA+AGG 0.510142 5_1:-11443790 None:intergenic
TCGGTGAGGGAAAAGAGAAA+GGG 0.515165 5_1:-11443906 None:intergenic
ATCATCTGCTATATCAGGCC+GGG 0.517909 5_1:-11445107 None:intergenic
GAACAGAGAAAGAGAGGTCA+AGG 0.521699 5_1:-11443841 None:intergenic
AAATCGGCGGCAGAGGTGGT+TGG 0.523503 5_1:-11443870 None:intergenic
AGGGTTTCAGTTTGAGAGAA+CGG 0.531395 5_1:-11443821 None:intergenic
TGAAAGGGTTTGTGAGAAGG+GGG 0.534696 5_1:-11443741 None:intergenic
ACATATCAAATGGACACCCT+TGG 0.536490 5_1:-11446171 None:intergenic
GCAAAGACGGTGGATAAGAA+TGG 0.536821 5_1:+11444658 Msa0717560:five_prime_UTR
CAGCAACTGCAATGGATAGG+TGG 0.537534 5_1:+11446125 Msa0717560:CDS
TGATGAACACAGACAGGGAC+TGG 0.540018 5_1:+11446840 Msa0717560:three_prime_UTR
TGAGAAGGCGCGTTGTGTGC+CGG 0.542492 5_1:-11444979 None:intergenic
GGCACCATCTGCATGGAAAG+AGG 0.548701 5_1:+11445469 Msa0717560:CDS
AGCAACTGCAATGGATAGGT+GGG 0.555214 5_1:+11446126 Msa0717560:CDS
AATCATCTGCTATATCAGGC+CGG 0.556123 5_1:-11445108 None:intergenic
GACTAAGAATGAATTACAAA+GGG 0.556790 5_1:-11443975 None:intergenic
TGAAAAGGCGCGTTGTGTGC+CGG 0.557765 5_1:-11444916 None:intergenic
GCACCATCTGCATGGAAAGA+GGG 0.557836 5_1:+11445470 Msa0717560:CDS
ATCAGAAGATAAAACTTCAC+CGG 0.561053 5_1:-11444146 None:intergenic
GGTGCGTGATGAACACAGAC+AGG 0.561080 5_1:+11446834 Msa0717560:three_prime_UTR
TCTTTGAATTTCGAGTAGGT+TGG 0.561301 5_1:+11444636 Msa0717560:five_prime_UTR
TGCTGCAGGAATTCACAGCC+CGG 0.568216 5_1:+11445089 Msa0717560:intron
ATCAATTCGAGAGGAAGATA+CGG 0.569775 5_1:-11444101 None:intergenic
ATGCCCTCTTTCCATGCAGA+TGG 0.572500 5_1:-11445473 None:intergenic
TGTAGTGAGTGAGTGAGATA+GGG 0.575875 5_1:+11444009 Msa0717560:five_prime_UTR
GAGAGATGAAAGGGTTTGAG+AGG 0.578164 5_1:-11443780 None:intergenic
ATCAACAGGAAATCTGTTGA+CGG 0.581442 5_1:-11446215 None:intergenic
TTTGGTCTGAGTTCTGAGAG+AGG 0.582217 5_1:+11444492 Msa0717560:five_prime_UTR
AGTCCAACAAATTATTCTAG+TGG 0.585826 5_1:-11446888 None:intergenic
TTACAGCAACTGCAATGGAT+AGG 0.587244 5_1:+11446122 Msa0717560:CDS
ACATATTACAGCAACTGCAA+TGG 0.587371 5_1:+11446117 Msa0717560:CDS
CGACTAAGAATGAATTACAA+AGG 0.590314 5_1:-11443976 None:intergenic
CTCACTCACTACAGCGAGAA+CGG 0.594898 5_1:-11443999 None:intergenic
ATCTGTGAACAGAGAAAGAG+AGG 0.604053 5_1:-11443847 None:intergenic
AAGTTAAGGCACCATCTGCA+TGG 0.608586 5_1:+11445462 Msa0717560:CDS
AAAACAAAGAAAGCAGTCGA+AGG 0.615661 5_1:-11444341 None:intergenic
ACACCTGAATACTTGTGAGA+AGG 0.616732 5_1:-11444994 None:intergenic
TGGTGAAATGTTAAAACTGG+TGG 0.617548 5_1:+11446969 Msa0717560:three_prime_UTR
TGATCATTCAAATTCTACTG+AGG 0.618347 5_1:+11444120 Msa0717560:five_prime_UTR
ATCTAAGAGAGAATCGGTGA+GGG 0.620102 5_1:-11443919 None:intergenic
GAGTAGGTTGGCAAAGACGG+TGG 0.628587 5_1:+11444648 Msa0717560:five_prime_UTR
TTGCAGAAGACGAAAGCGAA+CGG 0.630194 5_1:+11444058 Msa0717560:five_prime_UTR
TTGGGATCACAGTACAGCAT+AGG 0.632307 5_1:+11445221 Msa0717560:CDS
AGTTTGAGAGAACGGTGGAG+AGG 0.644306 5_1:-11443813 None:intergenic
AACAGAGAAAGAGAGGTCAA+GGG 0.644341 5_1:-11443840 None:intergenic
GTTTCAGTTTGAGAGAACGG+TGG 0.655796 5_1:-11443818 None:intergenic
TCGAGAGGAAGATACGGCAG+CGG 0.657456 5_1:-11444095 None:intergenic
AGGACGGATGAACTTGTCGA+TGG 0.659217 5_1:+11446082 Msa0717560:CDS
ATTTGAATGATCAATTCGAG+AGG 0.661945 5_1:-11444110 None:intergenic
ATAGAGCAATCAACTAACGA+AGG 0.684184 5_1:-11445196 None:intergenic
TATCTAAGAGAGAATCGGTG+AGG 0.685839 5_1:-11443920 None:intergenic
GTGCGTGATGAACACAGACA+GGG 0.708064 5_1:+11446835 Msa0717560:three_prime_UTR
GGCGAGGGGATAAACAACTG+AGG 0.741182 5_1:-11445166 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTGATTTTTATTTATGT+TGG - chr5_1:11445967-11445986 None:intergenic 10.0%
!!! TGTTTATTTTTGTTGATTTT+TGG + chr5_1:11444474-11444493 Msa0717560:five_prime_UTR 15.0%
!!! AATGTTACTGTTTTTAATTA+TGG + chr5_1:11444570-11444589 Msa0717560:five_prime_UTR 15.0%
!! ACTCTATGAAAAATAATTAT+GGG - chr5_1:11445375-11445394 None:intergenic 15.0%
!! AACTCTATGAAAAATAATTA+TGG - chr5_1:11445376-11445395 None:intergenic 15.0%
!! TTTCATGTATATATCTAATT+TGG - chr5_1:11446267-11446286 None:intergenic 15.0%
!!! ATTCTATATTGTTTTTACTT+TGG + chr5_1:11446718-11446737 Msa0717560:intron 15.0%
!!! TTTATCTTTTACTAACCAAT+TGG + chr5_1:11444413-11444432 Msa0717560:five_prime_UTR 20.0%
!! AATATAGATTTGTGATTATG+TGG + chr5_1:11444764-11444783 Msa0717560:five_prime_UTR 20.0%
!!! CTGTTTTGATTTTAATTTAG+TGG + chr5_1:11444825-11444844 Msa0717560:five_prime_UTR 20.0%
!!! ATAACATTTTAGAATAGTGT+AGG - chr5_1:11445292-11445311 None:intergenic 20.0%
!! TTATTTATTCTTGAGATATC+AGG + chr5_1:11445346-11445365 Msa0717560:intron 20.0%
!!! CTGCATTTTACTTTTATTTT+AGG + chr5_1:11445427-11445446 Msa0717560:intron 20.0%
!! AACATATATAGAGTTACTTA+AGG + chr5_1:11445519-11445538 Msa0717560:intron 20.0%
!!! AAATAACTGGTATTTTAAGA+GGG - chr5_1:11445581-11445600 None:intergenic 20.0%
!!! AAAATAACTGGTATTTTAAG+AGG - chr5_1:11445582-11445601 None:intergenic 20.0%
!! TACTAAAAAGTAAAACAAAC+AGG - chr5_1:11445650-11445669 None:intergenic 20.0%
!! AAAACCCAATATAACTAAAT+TGG + chr5_1:11445858-11445877 Msa0717560:intron 20.0%
!! CATTCCAATTTAGTTATATT+GGG - chr5_1:11445865-11445884 None:intergenic 20.0%
!! ACATTCCAATTTAGTTATAT+TGG - chr5_1:11445866-11445885 None:intergenic 20.0%
!!! TAAATATGTTTTTTCTCTCA+TGG + chr5_1:11446404-11446423 Msa0717560:intron 20.0%
!! AAAGAAACTAATTGTAAACA+TGG + chr5_1:11446449-11446468 Msa0717560:intron 20.0%
!! TTATTTCATCTAATTCTGTT+TGG + chr5_1:11446478-11446497 Msa0717560:intron 20.0%
!! AGACATAAATATTTAGTTGT+TGG + chr5_1:11446683-11446702 Msa0717560:intron 20.0%
!!! GTACTTGTAATTGTTATTTT+TGG + chr5_1:11446949-11446968 Msa0717560:three_prime_UTR 20.0%
!! TTTTAATAGACAAAAATCGG+CGG - chr5_1:11443886-11443905 None:intergenic 25.0%
!!! GGGTTTTAATAGACAAAAAT+CGG - chr5_1:11443889-11443908 None:intergenic 25.0%
! TTTCATATCTAAGAGAGAAT+CGG - chr5_1:11443928-11443947 None:intergenic 25.0%
! GACTAAGAATGAATTACAAA+GGG - chr5_1:11443978-11443997 None:intergenic 25.0%
!!! AGTTTTATCTTCTGATTGTA+AGG + chr5_1:11444153-11444172 Msa0717560:intron 25.0%
!! GTTTTATCTTCTGATTGTAA+GGG + chr5_1:11444154-11444173 Msa0717560:intron 25.0%
!!! TGACTTTTTTTTTACCTTCA+AGG + chr5_1:11444251-11444270 Msa0717560:intron 25.0%
!!! TAGGAATAAATTTGTTATGC+TGG + chr5_1:11444532-11444551 Msa0717560:five_prime_UTR 25.0%
!!! ATTTTCTTTGAATTTCGAGT+AGG + chr5_1:11444632-11444651 Msa0717560:five_prime_UTR 25.0%
!! ATTGAATGTTCTTGTTTCTT+TGG + chr5_1:11444864-11444883 Msa0717560:exon 25.0%
! TCAAATACTATACATAGATC+TGG - chr5_1:11445051-11445070 None:intergenic 25.0%
!! GTCTCTTGTTATAAAATAAC+TGG - chr5_1:11445594-11445613 None:intergenic 25.0%
!! TCAAAATTTTCAGCATTTTG+TGG + chr5_1:11445742-11445761 Msa0717560:intron 25.0%
! AAAATGAACTTAGTACATGA+TGG - chr5_1:11445924-11445943 None:intergenic 25.0%
!!! CACTAATTTCTGAAATTTAG+AGG - chr5_1:11446029-11446048 None:intergenic 25.0%
! GAACAAAATTAATCAATACC+TGG - chr5_1:11446243-11446262 None:intergenic 25.0%
! GTAGAAAACAACATTAATGA+TGG - chr5_1:11446291-11446310 None:intergenic 25.0%
!!! CATTAATGTTGTTTTCTACT+TGG + chr5_1:11446292-11446311 Msa0717560:intron 25.0%
! TCCACAAAATCATTATATGT+GGG - chr5_1:11446379-11446398 None:intergenic 25.0%
! TTCCACAAAATCATTATATG+TGG - chr5_1:11446380-11446399 None:intergenic 25.0%
!! TTTTGGTGAAATGTTAAAAC+TGG + chr5_1:11446966-11446985 Msa0717560:three_prime_UTR 25.0%
GAGAGAAGAAATTGTCAATT+TGG - chr5_1:11443695-11443714 None:intergenic 30.0%
!! TGATCTTTTGAGAGATGAAA+GGG - chr5_1:11443759-11443778 None:intergenic 30.0%
!! TTGATCTTTTGAGAGATGAA+AGG - chr5_1:11443760-11443779 None:intergenic 30.0%
CGACTAAGAATGAATTACAA+AGG - chr5_1:11443979-11443998 None:intergenic 30.0%
ATTTGAATGATCAATTCGAG+AGG - chr5_1:11444113-11444132 None:intergenic 30.0%
TGATCATTCAAATTCTACTG+AGG + chr5_1:11444120-11444139 Msa0717560:five_prime_UTR 30.0%
ATCAGAAGATAAAACTTCAC+CGG - chr5_1:11444149-11444168 None:intergenic 30.0%
!!! TTTTTTTTTACCTTCAAGGC+AGG + chr5_1:11444255-11444274 Msa0717560:intron 30.0%
!!! TTTTTTTTACCTTCAAGGCA+GGG + chr5_1:11444256-11444275 Msa0717560:intron 30.0%
! TTTTACTAACCAATTGGTTG+TGG + chr5_1:11444419-11444438 Msa0717560:five_prime_UTR 30.0%
TTTGTGATTATGTGGTGTTT+AGG + chr5_1:11444772-11444791 Msa0717560:five_prime_UTR 30.0%
TGAAGGATATAGTTGTGAAA+AGG - chr5_1:11444934-11444953 None:intergenic 30.0%
CTCAATCAAAACAGATATGA+AGG - chr5_1:11444951-11444970 None:intergenic 30.0%
!!! CTTCATATCTGTTTTGATTG+AGG + chr5_1:11444949-11444968 Msa0717560:CDS 30.0%
ATACTATACATAGATCTGGA+AGG - chr5_1:11445047-11445066 None:intergenic 30.0%
AAACAATCATCTGCTATATC+AGG - chr5_1:11445115-11445134 None:intergenic 30.0%
!!! AGCAGATGATTGTTTTTTGT+TGG + chr5_1:11445120-11445139 Msa0717560:CDS 30.0%
!!! ATTGTTTTTTGTTGGCTTCA+AGG + chr5_1:11445128-11445147 Msa0717560:CDS 30.0%
TTAGTTGATTGCTCTATGAT+TGG + chr5_1:11445202-11445221 Msa0717560:CDS 30.0%
TAGTTGATTGCTCTATGATT+GGG + chr5_1:11445203-11445222 Msa0717560:CDS 30.0%
TAAAATGCAGACATTTATGC+AGG - chr5_1:11445417-11445436 None:intergenic 30.0%
AGTAAAACAAACAGGTTTGA+AGG - chr5_1:11445642-11445661 None:intergenic 30.0%
!!! TATATTGGGTTTTTTCTCTC+AGG - chr5_1:11445851-11445870 None:intergenic 30.0%
!!! ATACTACTGCTTTTTCAGTT+TGG + chr5_1:11446053-11446072 Msa0717560:intron 30.0%
! CTGTAATATGTGAAGCATTA+GGG - chr5_1:11446108-11446127 None:intergenic 30.0%
!!! AATGTTGTTTTCTACTTGGA+AGG + chr5_1:11446296-11446315 Msa0717560:intron 30.0%
CCACAAAATCATTATATGTG+GGG - chr5_1:11446378-11446397 None:intergenic 30.0%
!!! AATAACAGCTCTTTTGGAAT+TGG - chr5_1:11446538-11446557 None:intergenic 30.0%
ATTCCAAAAGAGCTGTTATT+TGG + chr5_1:11446538-11446557 Msa0717560:intron 30.0%
! TAACCAAATAACAGCTCTTT+TGG - chr5_1:11446544-11446563 None:intergenic 30.0%
!! CTGATTTGTTCTAATTATGC+AGG + chr5_1:11446772-11446791 Msa0717560:intron 30.0%
! GTTCTAATTATGCAGGATTT+TGG + chr5_1:11446779-11446798 Msa0717560:intron 30.0%
! TTGCCACTAGAATAATTTGT+TGG + chr5_1:11446885-11446904 Msa0717560:three_prime_UTR 30.0%
AGTCCAACAAATTATTCTAG+TGG - chr5_1:11446891-11446910 None:intergenic 30.0%
TGATCTGTTGAGAGATGAAA+GGG - chr5_1:11443792-11443811 None:intergenic 35.0%
TTGATCTGTTGAGAGATGAA+AGG - chr5_1:11443793-11443812 None:intergenic 35.0%
ATGGTCGTTTCTCTTCATTT+CGG - chr5_1:11444039-11444058 None:intergenic 35.0%
ATCAATTCGAGAGGAAGATA+CGG - chr5_1:11444104-11444123 None:intergenic 35.0%
TCAAATTCTACTGAGGTTAC+CGG + chr5_1:11444127-11444146 Msa0717560:five_prime_UTR 35.0%
!!! TTTTTTTACCTTCAAGGCAG+GGG + chr5_1:11444257-11444276 Msa0717560:intron 35.0%
TCTGTCATAAAAAGCTGCTA+AGG + chr5_1:11444298-11444317 Msa0717560:five_prime_UTR 35.0%
AAGGTCTATCTGGATTGTTT+GGG - chr5_1:11444325-11444344 None:intergenic 35.0%
AAAACAAAGAAAGCAGTCGA+AGG - chr5_1:11444344-11444363 None:intergenic 35.0%
GGTTCAATAAGGAAAGTCAT+AGG + chr5_1:11444513-11444532 Msa0717560:five_prime_UTR 35.0%
! TCTTTGAATTTCGAGTAGGT+TGG + chr5_1:11444636-11444655 Msa0717560:five_prime_UTR 35.0%
! AATGTTCTTGTTTCTTTGGC+CGG + chr5_1:11444868-11444887 Msa0717560:exon 35.0%
TCAACTAACGAAGGAAAAAC+TGG - chr5_1:11445190-11445209 None:intergenic 35.0%
ATAGAGCAATCAACTAACGA+AGG - chr5_1:11445199-11445218 None:intergenic 35.0%
TTGTGCAATTGTATGTGTGT+GGG - chr5_1:11445257-11445276 None:intergenic 35.0%
ATTGTGCAATTGTATGTGTG+TGG - chr5_1:11445258-11445277 None:intergenic 35.0%
AACTTGTAGCCAATTCGATT+TGG + chr5_1:11445886-11445905 Msa0717560:intron 35.0%
AGAGCATAACCAAATCGAAT+TGG - chr5_1:11445898-11445917 None:intergenic 35.0%
! GCTGTAATATGTGAAGCATT+AGG - chr5_1:11446109-11446128 None:intergenic 35.0%
ACATATTACAGCAACTGCAA+TGG + chr5_1:11446117-11446136 Msa0717560:CDS 35.0%
GCAGCATCAAACATATCAAA+TGG - chr5_1:11446184-11446203 None:intergenic 35.0%
ATCAACAGGAAATCTGTTGA+CGG - chr5_1:11446218-11446237 None:intergenic 35.0%
ATCAATACCTGGATATCAAC+AGG - chr5_1:11446232-11446251 None:intergenic 35.0%
! CCCCACATATAATGATTTTG+TGG + chr5_1:11446375-11446394 Msa0717560:intron 35.0%
ACTTCCAAAGCAGTTAAGAA+TGG - chr5_1:11446606-11446625 None:intergenic 35.0%
GCTATGGTGTGAGATTTATA+TGG + chr5_1:11446813-11446832 Msa0717560:three_prime_UTR 35.0%
ATGAAAGGGTTTGTGAGAAG+GGG - chr5_1:11443745-11443764 None:intergenic 40.0%
GATGAAAGGGTTTGTGAGAA+GGG - chr5_1:11443746-11443765 None:intergenic 40.0%
AGATGAAAGGGTTTGTGAGA+AGG - chr5_1:11443747-11443766 None:intergenic 40.0%
AGGGTTTCAGTTTGAGAGAA+CGG - chr5_1:11443824-11443843 None:intergenic 40.0%
AACAGAGAAAGAGAGGTCAA+GGG - chr5_1:11443843-11443862 None:intergenic 40.0%
ATCTGTGAACAGAGAAAGAG+AGG - chr5_1:11443850-11443869 None:intergenic 40.0%
ATCTAAGAGAGAATCGGTGA+GGG - chr5_1:11443922-11443941 None:intergenic 40.0%
TATCTAAGAGAGAATCGGTG+AGG - chr5_1:11443923-11443942 None:intergenic 40.0%
TGTAGTGAGTGAGTGAGATA+GGG + chr5_1:11444009-11444028 Msa0717560:five_prime_UTR 40.0%
GAAGGTCTATCTGGATTGTT+TGG - chr5_1:11444326-11444345 None:intergenic 40.0%
!! GTTCTGAGAGAGGTTCAATA+AGG + chr5_1:11444502-11444521 Msa0717560:five_prime_UTR 40.0%
GCCTCAATCAAAACAAGATC+CGG - chr5_1:11444890-11444909 None:intergenic 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr5_1:11444897-11444916 Msa0717560:CDS 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr5_1:11444897-11444916 Msa0717560:CDS 40.0%
ACACCTGAATACTTGTGAGA+AGG - chr5_1:11444997-11445016 None:intergenic 40.0%
CAAGTATTCAGGTGTGATTG+TGG + chr5_1:11445002-11445021 Msa0717560:intron 40.0%
!! CTTATCACACTTTTTGCTGC+AGG + chr5_1:11445075-11445094 Msa0717560:intron 40.0%
AATCATCTGCTATATCAGGC+CGG - chr5_1:11445111-11445130 None:intergenic 40.0%
ATCACAGTACAGCATAGGTA+TGG + chr5_1:11445226-11445245 Msa0717560:intron 40.0%
GGCTCACTGTCTATAAGTTA+AGG + chr5_1:11445448-11445467 Msa0717560:intron 40.0%
CTATTTGGGCGATATATGGT+GGG - chr5_1:11445796-11445815 None:intergenic 40.0%
AGAGCTATTTGGGCGATATA+TGG - chr5_1:11445800-11445819 None:intergenic 40.0%
CGAAAGGCAAAGAGCTATTT+GGG - chr5_1:11445810-11445829 None:intergenic 40.0%
CCAAATAGCTCTTTGCCTTT+CGG + chr5_1:11445808-11445827 Msa0717560:intron 40.0%
CAAATAGCTCTTTGCCTTTC+GGG + chr5_1:11445809-11445828 Msa0717560:intron 40.0%
!!! CTGCTTTTTCAGTTTGGTGT+AGG + chr5_1:11446059-11446078 Msa0717560:intron 40.0%
!!! CTTTTTCAGTTTGGTGTAGG+AGG + chr5_1:11446062-11446081 Msa0717560:intron 40.0%
TTACAGCAACTGCAATGGAT+AGG + chr5_1:11446122-11446141 Msa0717560:CDS 40.0%
! AGATTGGACGAACTTTTCCA+AGG + chr5_1:11446154-11446173 Msa0717560:CDS 40.0%
! GATTGGACGAACTTTTCCAA+GGG + chr5_1:11446155-11446174 Msa0717560:CDS 40.0%
ACATATCAAATGGACACCCT+TGG - chr5_1:11446174-11446193 None:intergenic 40.0%
CAGATTTCCTGTTGATATCC+AGG + chr5_1:11446222-11446241 Msa0717560:CDS 40.0%
GGCTAGAAGATAGCAAAGTT+AGG + chr5_1:11446425-11446444 Msa0717560:intron 40.0%
AAGGTTAGAAGCTACATGGA+AGG - chr5_1:11446582-11446601 None:intergenic 40.0%
CAAAGCAGTTAAGAATGGCA+AGG - chr5_1:11446601-11446620 None:intergenic 40.0%
CTTGCCATTCTTAACTGCTT+TGG + chr5_1:11446599-11446618 Msa0717560:intron 40.0%
! TTGTGCTATTGTCCTGAAGT+AGG - chr5_1:11446651-11446670 None:intergenic 40.0%
!!! CAGGATTTTGGTTCTATGTG+AGG + chr5_1:11446791-11446810 Msa0717560:intron 40.0%
!! TTTGGTTCTATGTGAGGCTA+TGG + chr5_1:11446797-11446816 Msa0717560:exon 40.0%
TGAAAGGGTTTGTGAGAAGG+GGG - chr5_1:11443744-11443763 None:intergenic 45.0%
GAGAGATGAAAGGGTTTGAG+AGG - chr5_1:11443783-11443802 None:intergenic 45.0%
GTTTCAGTTTGAGAGAACGG+TGG - chr5_1:11443821-11443840 None:intergenic 45.0%
GAACAGAGAAAGAGAGGTCA+AGG - chr5_1:11443844-11443863 None:intergenic 45.0%
TCGGTGAGGGAAAAGAGAAA+GGG - chr5_1:11443909-11443928 None:intergenic 45.0%
ATCGGTGAGGGAAAAGAGAA+AGG - chr5_1:11443910-11443929 None:intergenic 45.0%
CTGTAGTGAGTGAGTGAGAT+AGG + chr5_1:11444008-11444027 Msa0717560:five_prime_UTR 45.0%
TTCGCTTTCGTCTTCTGCAA+TGG - chr5_1:11444058-11444077 None:intergenic 45.0%
TTGCAGAAGACGAAAGCGAA+CGG + chr5_1:11444058-11444077 Msa0717560:five_prime_UTR 45.0%
AAAGCAGTCGAAGGTCTATC+TGG - chr5_1:11444335-11444354 None:intergenic 45.0%
CGAACTTCACCACAACCAAT+TGG - chr5_1:11444431-11444450 None:intergenic 45.0%
!! TTTGGTCTGAGTTCTGAGAG+AGG + chr5_1:11444492-11444511 Msa0717560:five_prime_UTR 45.0%
! TTCGAGTAGGTTGGCAAAGA+CGG + chr5_1:11444645-11444664 Msa0717560:five_prime_UTR 45.0%
GCAAAGACGGTGGATAAGAA+TGG + chr5_1:11444658-11444677 Msa0717560:five_prime_UTR 45.0%
!!! GCCGGATCTTGTTTTGATTG+AGG + chr5_1:11444886-11444905 Msa0717560:CDS 45.0%
ATCATCTGCTATATCAGGCC+GGG - chr5_1:11445110-11445129 None:intergenic 45.0%
AACGAAGGAAAAACTGGCGA+GGG - chr5_1:11445184-11445203 None:intergenic 45.0%
TAACGAAGGAAAAACTGGCG+AGG - chr5_1:11445185-11445204 None:intergenic 45.0%
TTGGGATCACAGTACAGCAT+AGG + chr5_1:11445221-11445240 Msa0717560:CDS 45.0%
! AAGTTAAGGCACCATCTGCA+TGG + chr5_1:11445462-11445481 Msa0717560:CDS 45.0%
!! GATATATGGTGGGCGATACA+TGG - chr5_1:11445786-11445805 None:intergenic 45.0%
GCTATTTGGGCGATATATGG+TGG - chr5_1:11445797-11445816 None:intergenic 45.0%
CCGAAAGGCAAAGAGCTATT+TGG - chr5_1:11445811-11445830 None:intergenic 45.0%
CTCTTCTAATGACTCCCGAA+AGG - chr5_1:11445826-11445845 None:intergenic 45.0%
!! TTCAGTTTGGTGTAGGAGGA+CGG + chr5_1:11446066-11446085 Msa0717560:intron 45.0%
AGCAACTGCAATGGATAGGT+GGG + chr5_1:11446126-11446145 Msa0717560:CDS 45.0%
GGATAGGTGGGAATCAAGAT+TGG + chr5_1:11446138-11446157 Msa0717560:CDS 45.0%
TGTGGGGACAAAAAGAACCT+AGG - chr5_1:11446362-11446381 None:intergenic 45.0%
TGGCAAGGTTAGAAGCTACA+TGG - chr5_1:11446586-11446605 None:intergenic 45.0%
GCTATTGTCCTGAAGTAGGT+AGG - chr5_1:11446647-11446666 None:intergenic 45.0%
AGTTTGAGAGAACGGTGGAG+AGG - chr5_1:11443816-11443835 None:intergenic 50.0%
TAGACAAAAATCGGCGGCAG+AGG - chr5_1:11443880-11443899 None:intergenic 50.0%
CTCACTCACTACAGCGAGAA+CGG - chr5_1:11444002-11444021 None:intergenic 50.0%
CATTAAAGCCCCTGCCTTGA+AGG - chr5_1:11444268-11444287 None:intergenic 50.0%
GCGCCTTCTCACAAGTATTC+AGG + chr5_1:11444991-11445010 Msa0717560:CDS 50.0%
ACGAAGGAAAAACTGGCGAG+GGG - chr5_1:11445183-11445202 None:intergenic 50.0%
TGCAGACATTTATGCAGGCG+AGG - chr5_1:11445412-11445431 None:intergenic 50.0%
GCACCATCTGCATGGAAAGA+GGG + chr5_1:11445470-11445489 Msa0717560:CDS 50.0%
ATGCCCTCTTTCCATGCAGA+TGG - chr5_1:11445476-11445495 None:intergenic 50.0%
! TGGTGGGCGATACATGGAAA+GGG - chr5_1:11445780-11445799 None:intergenic 50.0%
!! ATGGTGGGCGATACATGGAA+AGG - chr5_1:11445781-11445800 None:intergenic 50.0%
AGGACGGATGAACTTGTCGA+TGG + chr5_1:11446082-11446101 Msa0717560:CDS 50.0%
CAGCAACTGCAATGGATAGG+TGG + chr5_1:11446125-11446144 Msa0717560:CDS 50.0%
TCTACTTGGAAGGCAACGAG+TGG + chr5_1:11446306-11446325 Msa0717560:intron 50.0%
! AAAGAACCTAGGCTGGTTGC+AGG - chr5_1:11446351-11446370 None:intergenic 50.0%
GGGACAAAAAGAACCTAGGC+TGG - chr5_1:11446358-11446377 None:intergenic 50.0%
CCTGCATGCTATAGCGAAGA+CGG - chr5_1:11446752-11446771 None:intergenic 50.0%
GTGCGTGATGAACACAGACA+GGG + chr5_1:11446835-11446854 Msa0717560:three_prime_UTR 50.0%
TGATGAACACAGACAGGGAC+TGG + chr5_1:11446840-11446859 Msa0717560:three_prime_UTR 50.0%
AAGGGGGAGTGAGAGTTGAG+GGG - chr5_1:11443728-11443747 None:intergenic 55.0%
GAAGGGGGAGTGAGAGTTGA+GGG - chr5_1:11443729-11443748 None:intergenic 55.0%
AGAAGGGGGAGTGAGAGTTG+AGG - chr5_1:11443730-11443749 None:intergenic 55.0%
ACAAAAATCGGCGGCAGAGG+TGG - chr5_1:11443877-11443896 None:intergenic 55.0%
TCGAGAGGAAGATACGGCAG+CGG - chr5_1:11444098-11444117 None:intergenic 55.0%
! CAAGGCAGGGGCTTTAATGC+TGG + chr5_1:11444269-11444288 Msa0717560:intron 55.0%
! GAGTAGGTTGGCAAAGACGG+TGG + chr5_1:11444648-11444667 Msa0717560:five_prime_UTR 55.0%
! TGAAAAGGCGCGTTGTGTGC+CGG - chr5_1:11444919-11444938 None:intergenic 55.0%
TGCTGCAGGAATTCACAGCC+CGG + chr5_1:11445089-11445108 Msa0717560:intron 55.0%
GGCGAGGGGATAAACAACTG+AGG - chr5_1:11445169-11445188 None:intergenic 55.0%
GCAGGCGAGGAAATCAGACA+TGG - chr5_1:11445399-11445418 None:intergenic 55.0%
GGCACCATCTGCATGGAAAG+AGG + chr5_1:11445469-11445488 Msa0717560:CDS 55.0%
GAACTTCCTGCAACCAGCCT+AGG + chr5_1:11446342-11446361 Msa0717560:intron 55.0%
CCTGAAGTAGGTAGGCACGT+TGG - chr5_1:11446639-11446658 None:intergenic 55.0%
CCAACGTGCCTACCTACTTC+AGG + chr5_1:11446636-11446655 Msa0717560:intron 55.0%
CCGTCTTCGCTATAGCATGC+AGG + chr5_1:11446749-11446768 Msa0717560:intron 55.0%
GGTGCGTGATGAACACAGAC+AGG + chr5_1:11446834-11446853 Msa0717560:three_prime_UTR 55.0%
AAATCGGCGGCAGAGGTGGT+TGG - chr5_1:11443873-11443892 None:intergenic 60.0%
! TGAGAAGGCGCGTTGTGTGC+CGG - chr5_1:11444982-11445001 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 11443691 11446990 11443691 ID=Msa0717560;Name=Msa0717560
chr5_1 mRNA 11443691 11446990 11443691 ID=Msa0717560-mRNA-1;Parent=Msa0717560;Name=Msa0717560-mRNA-1;_AED=0.04;_eAED=0.04;_QI=1071|0.6|0.83|0.83|0.2|0.16|6|179|175
chr5_1 exon 11443691 11444169 11443691 ID=Msa0717560-mRNA-1:exon:4640;Parent=Msa0717560-mRNA-1
chr5_1 exon 11444277 11445012 11444277 ID=Msa0717560-mRNA-1:exon:4641;Parent=Msa0717560-mRNA-1
chr5_1 exon 11445097 11445242 11445097 ID=Msa0717560-mRNA-1:exon:4642;Parent=Msa0717560-mRNA-1
chr5_1 exon 11445449 11445495 11445449 ID=Msa0717560-mRNA-1:exon:4643;Parent=Msa0717560-mRNA-1
chr5_1 exon 11446071 11446243 11446071 ID=Msa0717560-mRNA-1:exon:4644;Parent=Msa0717560-mRNA-1
chr5_1 exon 11446794 11446990 11446794 ID=Msa0717560-mRNA-1:exon:4645;Parent=Msa0717560-mRNA-1
chr5_1 five_prime_UTR 11443691 11444169 11443691 ID=Msa0717560-mRNA-1:five_prime_utr;Parent=Msa0717560-mRNA-1
chr5_1 five_prime_UTR 11444277 11444868 11444277 ID=Msa0717560-mRNA-1:five_prime_utr;Parent=Msa0717560-mRNA-1
chr5_1 CDS 11444869 11445012 11444869 ID=Msa0717560-mRNA-1:cds;Parent=Msa0717560-mRNA-1
chr5_1 CDS 11445097 11445242 11445097 ID=Msa0717560-mRNA-1:cds;Parent=Msa0717560-mRNA-1
chr5_1 CDS 11445449 11445495 11445449 ID=Msa0717560-mRNA-1:cds;Parent=Msa0717560-mRNA-1
chr5_1 CDS 11446071 11446243 11446071 ID=Msa0717560-mRNA-1:cds;Parent=Msa0717560-mRNA-1
chr5_1 CDS 11446794 11446811 11446794 ID=Msa0717560-mRNA-1:cds;Parent=Msa0717560-mRNA-1
chr5_1 three_prime_UTR 11446812 11446990 11446812 ID=Msa0717560-mRNA-1:three_prime_utr;Parent=Msa0717560-mRNA-1
Gene Sequence

>Msa0717560

ATGTTCTTGTTTCTTTGGCCGGATCTTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTTTCACAACTATATCCTTCATATCTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTCTCACAAGTATTCAGGAATTCACAGCCCGGCCTGATATAGCAGATGATTGTTTTTTGTTGGCTTCAAGGTGTATTCGCTACTGTCCTCAGTTGTTTATCCCCTCGCCAGTTTTTCCTTCGTTAGTTGATTGCTCTATGATTGGGATCACAGTACAGCATAGGCTCACTGTCTATAAGTTAAGGCACCATCTGCATGGAAAGAGGGCATTTTGGTGTAGGAGGACGGATGAACTTGTCGATGGCCCTAATGCTTCACATATTACAGCAACTGCAATGGATAGGTGGGAATCAAGATTGGACGAACTTTTCCAAGGGTGTCCATTTGATATGTTTGATGCTGCTTTATCAGATACCGTCAACAGATTTCCTGTTGATATCCAGGATTTTGGTTCTATGTGA
Protein sequence

>Msa0717560

MFLFLWPDLVLIEALFRHTTRLFTTISFISVLIEALFRHTTRLLTSIQEFTARPDIADDCFLLASRCIRYCPQLFIPSPVFPSLVDCSMIGITVQHRLTVYKLRHHLHGKRAFWCRRTDELVDGPNASHITATAMDRWESRLDELFQGCPFDMFDAALSDTVNRFPVDIQDFGSM*