AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1094520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1094520 MtrunA17_Chr4g0059761 83.333 78 13 0 28 105 740 817 2.27e-38 139
Msa1094520 MtrunA17_Chr5g0433601 90.323 62 4 1 114 173 179 240 1.53e-32 120
Msa1094520 MtrunA17_Chr3g0095661 74.194 62 14 1 114 173 138 199 5.56e-27 104
Msa1094520 MtrunA17_Chr5g0442671 69.355 62 17 1 114 173 131 192 8.92e-24 95.9
Msa1094520 MtrunA17_Chr3g0121311 59.677 62 23 1 114 173 127 188 6.94e-21 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1094520 AT5G62600.1 77.143 70 16 0 28 97 736 805 2.42e-32 122
Msa1094520 AT5G17230.3 77.419 62 12 1 114 173 182 243 1.94e-27 107
Msa1094520 AT5G17230.2 77.419 62 12 1 114 173 167 228 2.06e-27 106
Msa1094520 AT5G17230.1 77.419 62 12 1 114 173 167 228 2.06e-27 106
Msa1094520 AT5G17230.4 77.419 62 12 1 114 173 167 228 2.06e-27 106

Find 100 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTAATTATGCAGGATTT+TGG 0.187064 7_3:+16421761 Msa1094520:intron
GAGAGAAGAAATTGTCAATT+TGG 0.207441 7_3:-16418677 None:intergenic
ATTGAATGTTCTTGTTTCTT+TGG 0.213959 7_3:+16419851 Msa1094520:exon
AATGTTACTGTTCTTAATTA+TGG 0.293181 7_3:+16419557 Msa1094520:five_prime_UTR
GAAGGTCTATCTGGATTGTT+TGG 0.313321 7_3:-16419310 None:intergenic
CTGTAGTGAGTGAGTGAGAT+AGG 0.323027 7_3:+16418994 Msa1094520:five_prime_UTR
AAACAATCATCTGCTATATC+AGG 0.347089 7_3:-16420099 None:intergenic
GCTGTAATATGTGAAGCATT+AGG 0.352990 7_3:-16421094 None:intergenic
AAAGCAGTCGAAGGTCTATC+TGG 0.356524 7_3:-16419319 None:intergenic
TTAGTTGATTGCTCTATGAT+TGG 0.367385 7_3:+16420189 Msa1094520:CDS
GCGCCTTCTCACAAGTATTC+AGG 0.382690 7_3:+16419978 Msa1094520:CDS
CTGTAATATGTGAAGCATTA+GGG 0.386843 7_3:-16421093 None:intergenic
TCAAATTCTACTGAGGTTAC+CGG 0.399273 7_3:+16419113 Msa1094520:five_prime_UTR
GATGATAGGGTTTGTGAGAA+GGG 0.402280 7_3:-16418728 None:intergenic
TAGTTGATTGCTCTATGATT+GGG 0.404013 7_3:+16420190 Msa1094520:CDS
CAGATTTCCTGTTGATATCC+AGG 0.418869 7_3:+16421204 Msa1094520:CDS
ATCAATACCTGGATATCAAC+AGG 0.418894 7_3:-16421211 None:intergenic
TTGCCACTAGAATAATTTGT+TGG 0.420265 7_3:+16421867 Msa1094520:three_prime_UTR
GGCTCACTGTCTATAAGTTA+AGG 0.428171 7_3:+16420435 Msa1094520:intron
AATGTTCTTGTTTCTTTGGC+CGG 0.429116 7_3:+16419855 Msa1094520:exon
ATCGGTGAGGGAAAAGAGAA+AGG 0.434137 7_3:-16418893 None:intergenic
CGAACTTCACCACAACCAAT+TGG 0.435988 7_3:-16419415 None:intergenic
TAGGAATAAATTTGTTATGC+TGG 0.438201 7_3:+16419519 Msa1094520:five_prime_UTR
TTTGTGATTATGTGGTGTTT+AGG 0.443685 7_3:+16419759 Msa1094520:five_prime_UTR
CAAGGCAGGGGCTTTAATGC+TGG 0.450060 7_3:+16419256 Msa1094520:intron
GCCTCAATCAAAACAAGATC+CGG 0.450430 7_3:-16419874 None:intergenic
GCAGCATCAAACATATCAAA+TGG 0.454928 7_3:-16421163 None:intergenic
AGAAGAAATTGTCAATTTGG+AGG 0.462443 7_3:-16418674 None:intergenic
TTTGGTTCTATGTGAGGCTA+TGG 0.465655 7_3:+16421779 Msa1094520:exon
GCTATGGTGTGAGATTTATA+TGG 0.465941 7_3:+16421795 Msa1094520:three_prime_UTR
GTTCTGAGAGAGGTTCAATA+AGG 0.468857 7_3:+16419489 Msa1094520:five_prime_UTR
AATATAGATTTGTGATTATG+TGG 0.471849 7_3:+16419751 Msa1094520:five_prime_UTR
AAGGTCTATCTGGATTGTTT+GGG 0.471918 7_3:-16419309 None:intergenic
TTCGCTTTCGTCTTCTGCAA+CGG 0.474504 7_3:-16419041 None:intergenic
ACGGTCGTTTCTCTTCATTT+CGG 0.476121 7_3:-16419022 None:intergenic
GGATAGGTGGGAATCAAGAT+TGG 0.482924 7_3:+16421120 Msa1094520:CDS
AGATGATAGGGTTTGTGAGA+AGG 0.486161 7_3:-16418729 None:intergenic
CTCAATCAAAACAGATATGA+AGG 0.488090 7_3:-16419935 None:intergenic
TGATCGGTTGAGAGATGAAA+GGG 0.489439 7_3:-16418774 None:intergenic
TTCGAGTAGGTTGGCAAAGA+CGG 0.491654 7_3:+16419632 Msa1094520:five_prime_UTR
AATCGGCGGCGGGAGGTGGT+TGG 0.491720 7_3:-16418855 None:intergenic
TTCAGTTTGGTGTAGGAGGA+CGG 0.496266 7_3:+16421054 Msa1094520:intron
TTTCATATCTAAGAGAGAAT+CGG 0.497576 7_3:-16418911 None:intergenic
TGAAGGATATAGTTGTGAAA+AGG 0.504325 7_3:-16419918 None:intergenic
GAGAGATGAAAGGGTTTGAG+AGG 0.505732 7_3:-16418765 None:intergenic
GGTTCAATAAGGAAAGTCAT+AGG 0.506604 7_3:+16419500 Msa1094520:five_prime_UTR
GAAGAAATTGTCAATTTGGA+GGG 0.508671 7_3:-16418673 None:intergenic
ATGATAGGGTTTGTGAGAAG+GGG 0.515062 7_3:-16418727 None:intergenic
TCGGTGAGGGAAAAGAGAAA+GGG 0.515165 7_3:-16418892 None:intergenic
ATCATCTGCTATATCAGGCC+GGG 0.517909 7_3:-16420094 None:intergenic
ACATATCAAATGGACGCCCT+TGG 0.519572 7_3:-16421153 None:intergenic
GAACAGAGAAAGAGAGGTCA+AGG 0.521699 7_3:-16418826 None:intergenic
AGGGTTTCAGTTTGAGAGAA+CGG 0.531395 7_3:-16418806 None:intergenic
GCAAAGACGGTGGATAAGAA+TGG 0.536821 7_3:+16419645 Msa1094520:five_prime_UTR
TGATGAACACAGACAGGGAC+TGG 0.540018 7_3:+16421822 Msa1094520:three_prime_UTR
TGAGAAGGCGCGTTGTGTGC+CGG 0.542492 7_3:-16419966 None:intergenic
TGATAGGGTTTGTGAGAAGG+GGG 0.545695 7_3:-16418726 None:intergenic
GGCACCATCTGCATGGAAAG+AGG 0.548701 7_3:+16420456 Msa1094520:CDS
TGCTTCACATATTACAGCAA+TGG 0.548985 7_3:+16421099 Msa1094520:CDS
AATCATCTGCTATATCAGGC+CGG 0.556123 7_3:-16420095 None:intergenic
GACTAAGAATGAATTACAAA+GGG 0.556790 7_3:-16418961 None:intergenic
GCACCATCTGCATGGAAAGA+GGG 0.557836 7_3:+16420457 Msa1094520:CDS
ATCAGAAGATAAAACTTCAC+CGG 0.561053 7_3:-16419132 None:intergenic
GGTGCGTGATGAACACAGAC+AGG 0.561080 7_3:+16421816 Msa1094520:three_prime_UTR
TCTTTGAATTTCGAGTAGGT+TGG 0.561301 7_3:+16419623 Msa1094520:five_prime_UTR
AGAACGGTGGAGAGGTTGAT+CGG 0.565965 7_3:-16418790 None:intergenic
TGCTGCAGGAATTCACAGCC+CGG 0.568216 7_3:+16420076 Msa1094520:intron
ATCAATTCGAGAGGAAGATA+CGG 0.569775 7_3:-16419087 None:intergenic
ATGCCCTCTTTCCATGCAGA+TGG 0.572500 7_3:-16420460 None:intergenic
TGTAGTGAGTGAGTGAGATA+GGG 0.575875 7_3:+16418995 Msa1094520:five_prime_UTR
ATCAACAGGAAATCTGTTGA+CGG 0.581442 7_3:-16421197 None:intergenic
TTTGGTCTGAGTTCTGAGAG+AGG 0.582217 7_3:+16419479 Msa1094520:five_prime_UTR
TATTACAGCAATGGATAGGT+GGG 0.584644 7_3:+16421108 Msa1094520:CDS
AGTCCAACAAATTATTCTAG+TGG 0.585826 7_3:-16421870 None:intergenic
TTGATCGGTTGAGAGATGAA+AGG 0.585978 7_3:-16418775 None:intergenic
CGACTAAGAATGAATTACAA+AGG 0.590314 7_3:-16418962 None:intergenic
TGAAAAGGCGCATTGTGTGC+CGG 0.591934 7_3:-16419903 None:intergenic
CTCACTCACTACAGCGAGAA+CGG 0.594898 7_3:-16418985 None:intergenic
ATATTACAGCAATGGATAGG+TGG 0.596045 7_3:+16421107 Msa1094520:CDS
ATCTGTGAACAGAGAAAGAG+AGG 0.604053 7_3:-16418832 None:intergenic
AAGTTAAGGCACCATCTGCA+TGG 0.608586 7_3:+16420449 Msa1094520:CDS
AAAACAAAGAAAGCAGTCGA+AGG 0.615661 7_3:-16419328 None:intergenic
ACACCTGAATACTTGTGAGA+AGG 0.616732 7_3:-16419981 None:intergenic
CACATATTACAGCAATGGAT+AGG 0.617205 7_3:+16421104 Msa1094520:CDS
TGGTGAAATGTTAAAACTGG+TGG 0.617548 7_3:+16421951 Msa1094520:three_prime_UTR
TGATCATTCAAATTCTACTG+AGG 0.618347 7_3:+16419106 Msa1094520:five_prime_UTR
ATCTAAGAGAGAATCGGTGA+GGG 0.620102 7_3:-16418905 None:intergenic
GAGTAGGTTGGCAAAGACGG+TGG 0.628587 7_3:+16419635 Msa1094520:five_prime_UTR
TTGCAGAAGACGAAAGCGAA+CGG 0.630604 7_3:+16419044 Msa1094520:five_prime_UTR
TTGGGATCACAGTACAGCAT+AGG 0.632307 7_3:+16420208 Msa1094520:CDS
AGTTTGAGAGAACGGTGGAG+AGG 0.644306 7_3:-16418798 None:intergenic
AACAGAGAAAGAGAGGTCAA+GGG 0.644341 7_3:-16418825 None:intergenic
GTTTCAGTTTGAGAGAACGG+TGG 0.655796 7_3:-16418803 None:intergenic
TCGAGAGGAAGATACGGCAG+CGG 0.657456 7_3:-16419081 None:intergenic
AGGACGGATGAACTTGTCGA+TGG 0.659217 7_3:+16421070 Msa1094520:CDS
ATTTGAATGATCAATTCGAG+AGG 0.661945 7_3:-16419096 None:intergenic
ATAGAGCAATCAACTAACGA+AGG 0.684184 7_3:-16420183 None:intergenic
TATCTAAGAGAGAATCGGTG+AGG 0.685839 7_3:-16418906 None:intergenic
GTGCGTGATGAACACAGACA+GGG 0.708064 7_3:+16421817 Msa1094520:three_prime_UTR
GGCGAGGGGATAAACAACTG+AGG 0.741182 7_3:-16420153 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTGATTTTTATTTATGT+TGG - chr7_3:16420955-16420974 None:intergenic 10.0%
!! AAAAATCAACAAAAATAAAC+AGG - chr7_3:16419462-16419481 None:intergenic 15.0%
!!! TGTTTATTTTTGTTGATTTT+TGG + chr7_3:16419461-16419480 Msa1094520:five_prime_UTR 15.0%
!! ACTGTATGAAAAATAATTAT+GGG - chr7_3:16420362-16420381 None:intergenic 15.0%
!! AACTGTATGAAAAATAATTA+TGG - chr7_3:16420363-16420382 None:intergenic 15.0%
!! AATTCCAATTTAGTTATATT+GGG - chr7_3:16420853-16420872 None:intergenic 15.0%
!! AAATTCCAATTTAGTTATAT+TGG - chr7_3:16420854-16420873 None:intergenic 15.0%
!! TTTCATGTATATATCTAATT+TGG - chr7_3:16421249-16421268 None:intergenic 15.0%
!!! ATTCTATATTGTTTTTACTT+TGG + chr7_3:16421700-16421719 Msa1094520:intron 15.0%
!!! TTTATCTTTTACTAACCAAT+TGG + chr7_3:16419400-16419419 Msa1094520:five_prime_UTR 20.0%
!! AATGTTACTGTTCTTAATTA+TGG + chr7_3:16419557-16419576 Msa1094520:five_prime_UTR 20.0%
!! AATATAGATTTGTGATTATG+TGG + chr7_3:16419751-16419770 Msa1094520:five_prime_UTR 20.0%
!!! CTGTTTTGATTTTAATTTAG+TGG + chr7_3:16419812-16419831 Msa1094520:five_prime_UTR 20.0%
!! TTATTTATTCTTGAGATATC+AGG + chr7_3:16420333-16420352 Msa1094520:intron 20.0%
!!! CTGCATTTTACTTTTATTTT+AGG + chr7_3:16420414-16420433 Msa1094520:intron 20.0%
!! AACATATATAGAGTTACTTA+AGG + chr7_3:16420506-16420525 Msa1094520:intron 20.0%
!!! AAATAACTGGTATTTTAAGA+GGG - chr7_3:16420568-16420587 None:intergenic 20.0%
!!! AAAATAACTGGTATTTTAAG+AGG - chr7_3:16420569-16420588 None:intergenic 20.0%
!! TACTAAAAAGTAAAACAAAC+AGG - chr7_3:16420638-16420657 None:intergenic 20.0%
!! AAAACCCAATATAACTAAAT+TGG + chr7_3:16420846-16420865 Msa1094520:intron 20.0%
!!! TAAATATGTTTTTTCTCTCA+TGG + chr7_3:16421386-16421405 Msa1094520:intron 20.0%
!! AAAGAAACTAATTGTAAACA+TGG + chr7_3:16421431-16421450 Msa1094520:intron 20.0%
!! TTATTTCATCTAATTCTGTT+TGG + chr7_3:16421460-16421479 Msa1094520:intron 20.0%
!! AGACATAAATATTTAGTTGT+TGG + chr7_3:16421665-16421684 Msa1094520:intron 20.0%
!!! GTACTTGTAATTGTTATTTT+TGG + chr7_3:16421931-16421950 Msa1094520:three_prime_UTR 20.0%
!! TTTTAATAGACAAAAATCGG+CGG - chr7_3:16418872-16418891 None:intergenic 25.0%
!!! GGGTTTTAATAGACAAAAAT+CGG - chr7_3:16418875-16418894 None:intergenic 25.0%
! TTTCATATCTAAGAGAGAAT+CGG - chr7_3:16418914-16418933 None:intergenic 25.0%
! GACTAAGAATGAATTACAAA+GGG - chr7_3:16418964-16418983 None:intergenic 25.0%
!!! AGTTTTATCTTCTGATTGTA+AGG + chr7_3:16419139-16419158 Msa1094520:intron 25.0%
!! GTTTTATCTTCTGATTGTAA+GGG + chr7_3:16419140-16419159 Msa1094520:intron 25.0%
!!! GACTTTTTTTTTTACCTTCA+AGG + chr7_3:16419238-16419257 Msa1094520:intron 25.0%
!!! TAGGAATAAATTTGTTATGC+TGG + chr7_3:16419519-16419538 Msa1094520:five_prime_UTR 25.0%
!!! ATTTTCTTTGAATTTCGAGT+AGG + chr7_3:16419619-16419638 Msa1094520:five_prime_UTR 25.0%
!! ATTGAATGTTCTTGTTTCTT+TGG + chr7_3:16419851-16419870 Msa1094520:exon 25.0%
! TCAAATACTATACATAGATC+TGG - chr7_3:16420038-16420057 None:intergenic 25.0%
!!! ATACCATTTTAGAATAGTGT+AGG - chr7_3:16420279-16420298 None:intergenic 25.0%
!! GTCTCTTGTTATAAAATAAC+TGG - chr7_3:16420581-16420600 None:intergenic 25.0%
!! TCAAAATTTTCAGCATTTTG+TGG + chr7_3:16420730-16420749 Msa1094520:intron 25.0%
! AAAATGAACTTAGTACATGA+TGG - chr7_3:16420912-16420931 None:intergenic 25.0%
! GTATATTTGACATCTGATTA+GGG - chr7_3:16420978-16420997 None:intergenic 25.0%
! TGTATATTTGACATCTGATT+AGG - chr7_3:16420979-16420998 None:intergenic 25.0%
!!! CACTAATTTCTGAAATTTAG+AGG - chr7_3:16421017-16421036 None:intergenic 25.0%
! GAACAAAATTAATCAATACC+TGG - chr7_3:16421225-16421244 None:intergenic 25.0%
! GTAGAAAACAACATTAATGA+TGG - chr7_3:16421273-16421292 None:intergenic 25.0%
!!! AATGTTGTTTTCTACTTGAA+AGG + chr7_3:16421278-16421297 Msa1094520:intron 25.0%
! TCCACAAAATCATTATATGT+GGG - chr7_3:16421361-16421380 None:intergenic 25.0%
! TTCCACAAAATCATTATATG+TGG - chr7_3:16421362-16421381 None:intergenic 25.0%
!! TTTTGGTGAAATGTTAAAAC+TGG + chr7_3:16421948-16421967 Msa1094520:three_prime_UTR 25.0%
GAGAGAAGAAATTGTCAATT+TGG - chr7_3:16418680-16418699 None:intergenic 30.0%
!! TGATCTTTTGAGAGATGATA+GGG - chr7_3:16418744-16418763 None:intergenic 30.0%
!! TTGATCTTTTGAGAGATGAT+AGG - chr7_3:16418745-16418764 None:intergenic 30.0%
CGACTAAGAATGAATTACAA+AGG - chr7_3:16418965-16418984 None:intergenic 30.0%
ATTTGAATGATCAATTCGAG+AGG - chr7_3:16419099-16419118 None:intergenic 30.0%
TGATCATTCAAATTCTACTG+AGG + chr7_3:16419106-16419125 Msa1094520:five_prime_UTR 30.0%
ATCAGAAGATAAAACTTCAC+CGG - chr7_3:16419135-16419154 None:intergenic 30.0%
!!! TTTTTTTTTACCTTCAAGGC+AGG + chr7_3:16419242-16419261 Msa1094520:intron 30.0%
!!! TTTTTTTTACCTTCAAGGCA+GGG + chr7_3:16419243-16419262 Msa1094520:intron 30.0%
! TTTTACTAACCAATTGGTTG+TGG + chr7_3:16419406-16419425 Msa1094520:five_prime_UTR 30.0%
TTTGTGATTATGTGGTGTTT+AGG + chr7_3:16419759-16419778 Msa1094520:five_prime_UTR 30.0%
TGAAGGATATAGTTGTGAAA+AGG - chr7_3:16419921-16419940 None:intergenic 30.0%
CTCAATCAAAACAGATATGA+AGG - chr7_3:16419938-16419957 None:intergenic 30.0%
!!! CTTCATATCTGTTTTGATTG+AGG + chr7_3:16419936-16419955 Msa1094520:CDS 30.0%
ATACTATACATAGATCTGGA+AGG - chr7_3:16420034-16420053 None:intergenic 30.0%
AAACAATCATCTGCTATATC+AGG - chr7_3:16420102-16420121 None:intergenic 30.0%
!!! AGCAGATGATTGTTTTTTGT+TGG + chr7_3:16420107-16420126 Msa1094520:CDS 30.0%
!!! ATTGTTTTTTGTTGGCTTCA+AGG + chr7_3:16420115-16420134 Msa1094520:CDS 30.0%
TTAGTTGATTGCTCTATGAT+TGG + chr7_3:16420189-16420208 Msa1094520:CDS 30.0%
TAGTTGATTGCTCTATGATT+GGG + chr7_3:16420190-16420209 Msa1094520:CDS 30.0%
! CATCCTACACTATTCTAAAA+TGG + chr7_3:16420273-16420292 Msa1094520:intron 30.0%
TAAAATGCAGACATTTATGC+AGG - chr7_3:16420404-16420423 None:intergenic 30.0%
AGTAAAACAAACAGGTTTGA+AGG - chr7_3:16420630-16420649 None:intergenic 30.0%
!!! TATATTGGGTTTTTTCTCTC+AGG - chr7_3:16420839-16420858 None:intergenic 30.0%
!!! ATACTACTGCTTTTTCAGTT+TGG + chr7_3:16421041-16421060 Msa1094520:intron 30.0%
! CTGTAATATGTGAAGCATTA+GGG - chr7_3:16421096-16421115 None:intergenic 30.0%
CCACAAAATCATTATATGTG+GGG - chr7_3:16421360-16421379 None:intergenic 30.0%
!!! AATAACAGCTCTTTTGGAAT+TGG - chr7_3:16421520-16421539 None:intergenic 30.0%
ATTCCAAAAGAGCTGTTATT+TGG + chr7_3:16421520-16421539 Msa1094520:intron 30.0%
! TAACCAAATAACAGCTCTTT+TGG - chr7_3:16421526-16421545 None:intergenic 30.0%
!! CTGATTTGTTCTAATTATGC+AGG + chr7_3:16421754-16421773 Msa1094520:intron 30.0%
! GTTCTAATTATGCAGGATTT+TGG + chr7_3:16421761-16421780 Msa1094520:intron 30.0%
! TTGCCACTAGAATAATTTGT+TGG + chr7_3:16421867-16421886 Msa1094520:three_prime_UTR 30.0%
AGTCCAACAAATTATTCTAG+TGG - chr7_3:16421873-16421892 None:intergenic 30.0%
ATCAATTCGAGAGGAAGATA+CGG - chr7_3:16419090-16419109 None:intergenic 35.0%
TCAAATTCTACTGAGGTTAC+CGG + chr7_3:16419113-16419132 Msa1094520:five_prime_UTR 35.0%
!!! TTTTTTTACCTTCAAGGCAG+GGG + chr7_3:16419244-16419263 Msa1094520:intron 35.0%
TCTGTCATAAAAAGCTGCTA+AGG + chr7_3:16419285-16419304 Msa1094520:five_prime_UTR 35.0%
AAGGTCTATCTGGATTGTTT+GGG - chr7_3:16419312-16419331 None:intergenic 35.0%
AAAACAAAGAAAGCAGTCGA+AGG - chr7_3:16419331-16419350 None:intergenic 35.0%
GGTTCAATAAGGAAAGTCAT+AGG + chr7_3:16419500-16419519 Msa1094520:five_prime_UTR 35.0%
! TCTTTGAATTTCGAGTAGGT+TGG + chr7_3:16419623-16419642 Msa1094520:five_prime_UTR 35.0%
! AATGTTCTTGTTTCTTTGGC+CGG + chr7_3:16419855-16419874 Msa1094520:exon 35.0%
TCAACTAACGAAGGAAAAAC+TGG - chr7_3:16420177-16420196 None:intergenic 35.0%
ATAGAGCAATCAACTAACGA+AGG - chr7_3:16420186-16420205 None:intergenic 35.0%
TTGTGCAATTGTATGTGTGT+GGG - chr7_3:16420244-16420263 None:intergenic 35.0%
ATTGTGCAATTGTATGTGTG+TGG - chr7_3:16420245-16420264 None:intergenic 35.0%
AACTTGTAGCCAATTCGATT+TGG + chr7_3:16420874-16420893 Msa1094520:intron 35.0%
AGAGCATAACCAAATCGAAT+TGG - chr7_3:16420886-16420905 None:intergenic 35.0%
! GCTGTAATATGTGAAGCATT+AGG - chr7_3:16421097-16421116 None:intergenic 35.0%
TGCTTCACATATTACAGCAA+TGG + chr7_3:16421099-16421118 Msa1094520:CDS 35.0%
CACATATTACAGCAATGGAT+AGG + chr7_3:16421104-16421123 Msa1094520:CDS 35.0%
ATATTACAGCAATGGATAGG+TGG + chr7_3:16421107-16421126 Msa1094520:CDS 35.0%
TATTACAGCAATGGATAGGT+GGG + chr7_3:16421108-16421127 Msa1094520:CDS 35.0%
GCAGCATCAAACATATCAAA+TGG - chr7_3:16421166-16421185 None:intergenic 35.0%
ATCAACAGGAAATCTGTTGA+CGG - chr7_3:16421200-16421219 None:intergenic 35.0%
ATCAATACCTGGATATCAAC+AGG - chr7_3:16421214-16421233 None:intergenic 35.0%
! CCCCACATATAATGATTTTG+TGG + chr7_3:16421357-16421376 Msa1094520:intron 35.0%
ACTTCCAAAGCAGTTAAGAA+TGG - chr7_3:16421588-16421607 None:intergenic 35.0%
GCTATGGTGTGAGATTTATA+TGG + chr7_3:16421795-16421814 Msa1094520:three_prime_UTR 35.0%
ATGATAGGGTTTGTGAGAAG+GGG - chr7_3:16418730-16418749 None:intergenic 40.0%
GATGATAGGGTTTGTGAGAA+GGG - chr7_3:16418731-16418750 None:intergenic 40.0%
AGATGATAGGGTTTGTGAGA+AGG - chr7_3:16418732-16418751 None:intergenic 40.0%
TGATCGGTTGAGAGATGAAA+GGG - chr7_3:16418777-16418796 None:intergenic 40.0%
TTGATCGGTTGAGAGATGAA+AGG - chr7_3:16418778-16418797 None:intergenic 40.0%
AGGGTTTCAGTTTGAGAGAA+CGG - chr7_3:16418809-16418828 None:intergenic 40.0%
AACAGAGAAAGAGAGGTCAA+GGG - chr7_3:16418828-16418847 None:intergenic 40.0%
ATCTGTGAACAGAGAAAGAG+AGG - chr7_3:16418835-16418854 None:intergenic 40.0%
TAATAGACAAAAATCGGCGG+CGG - chr7_3:16418869-16418888 None:intergenic 40.0%
ATCTAAGAGAGAATCGGTGA+GGG - chr7_3:16418908-16418927 None:intergenic 40.0%
TATCTAAGAGAGAATCGGTG+AGG - chr7_3:16418909-16418928 None:intergenic 40.0%
TGTAGTGAGTGAGTGAGATA+GGG + chr7_3:16418995-16419014 Msa1094520:five_prime_UTR 40.0%
ACGGTCGTTTCTCTTCATTT+CGG - chr7_3:16419025-16419044 None:intergenic 40.0%
GAAGGTCTATCTGGATTGTT+TGG - chr7_3:16419313-16419332 None:intergenic 40.0%
!! GTTCTGAGAGAGGTTCAATA+AGG + chr7_3:16419489-16419508 Msa1094520:five_prime_UTR 40.0%
GCCTCAATCAAAACAAGATC+CGG - chr7_3:16419877-16419896 None:intergenic 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr7_3:16419884-16419903 Msa1094520:CDS 40.0%
!! TTTTGATTGAGGCGCTTTTC+CGG + chr7_3:16419884-16419903 Msa1094520:CDS 40.0%
ACACCTGAATACTTGTGAGA+AGG - chr7_3:16419984-16420003 None:intergenic 40.0%
CAAGTATTCAGGTGTGATTG+TGG + chr7_3:16419989-16420008 Msa1094520:intron 40.0%
!! CTTATCACACTTTTTGCTGC+AGG + chr7_3:16420062-16420081 Msa1094520:intron 40.0%
AATCATCTGCTATATCAGGC+CGG - chr7_3:16420098-16420117 None:intergenic 40.0%
ATCACAGTACAGCATAGGTA+TGG + chr7_3:16420213-16420232 Msa1094520:intron 40.0%
GGCTCACTGTCTATAAGTTA+AGG + chr7_3:16420435-16420454 Msa1094520:intron 40.0%
CTATTTGGGCGATATATGGT+GGG - chr7_3:16420784-16420803 None:intergenic 40.0%
AGAGCTATTTGGGCGATATA+TGG - chr7_3:16420788-16420807 None:intergenic 40.0%
!!! CTGCTTTTTCAGTTTGGTGT+AGG + chr7_3:16421047-16421066 Msa1094520:intron 40.0%
!!! CTTTTTCAGTTTGGTGTAGG+AGG + chr7_3:16421050-16421069 Msa1094520:intron 40.0%
! AGATTGGACGAACTTTTCCA+AGG + chr7_3:16421136-16421155 Msa1094520:CDS 40.0%
! GATTGGACGAACTTTTCCAA+GGG + chr7_3:16421137-16421156 Msa1094520:CDS 40.0%
CAGATTTCCTGTTGATATCC+AGG + chr7_3:16421204-16421223 Msa1094520:CDS 40.0%
TCTACTTGAAAGGCAACAAG+TGG + chr7_3:16421288-16421307 Msa1094520:intron 40.0%
GGCTAGAAGATAGCAAAGTT+AGG + chr7_3:16421407-16421426 Msa1094520:intron 40.0%
AAGGTTAGAAGCTACATGGA+AGG - chr7_3:16421564-16421583 None:intergenic 40.0%
CAAAGCAGTTAAGAATGGCA+AGG - chr7_3:16421583-16421602 None:intergenic 40.0%
CTTGCCATTCTTAACTGCTT+TGG + chr7_3:16421581-16421600 Msa1094520:intron 40.0%
! TTGTGCTATTGTCCTGAAGT+AGG - chr7_3:16421633-16421652 None:intergenic 40.0%
!!! CAGGATTTTGGTTCTATGTG+AGG + chr7_3:16421773-16421792 Msa1094520:intron 40.0%
!! TTTGGTTCTATGTGAGGCTA+TGG + chr7_3:16421779-16421798 Msa1094520:exon 40.0%
TGATAGGGTTTGTGAGAAGG+GGG - chr7_3:16418729-16418748 None:intergenic 45.0%
GAGAGATGAAAGGGTTTGAG+AGG - chr7_3:16418768-16418787 None:intergenic 45.0%
GTTTCAGTTTGAGAGAACGG+TGG - chr7_3:16418806-16418825 None:intergenic 45.0%
GAACAGAGAAAGAGAGGTCA+AGG - chr7_3:16418829-16418848 None:intergenic 45.0%
AATAGACAAAAATCGGCGGC+GGG - chr7_3:16418868-16418887 None:intergenic 45.0%
TCGGTGAGGGAAAAGAGAAA+GGG - chr7_3:16418895-16418914 None:intergenic 45.0%
ATCGGTGAGGGAAAAGAGAA+AGG - chr7_3:16418896-16418915 None:intergenic 45.0%
CTGTAGTGAGTGAGTGAGAT+AGG + chr7_3:16418994-16419013 Msa1094520:five_prime_UTR 45.0%
TTCGCTTTCGTCTTCTGCAA+CGG - chr7_3:16419044-16419063 None:intergenic 45.0%
TTGCAGAAGACGAAAGCGAA+CGG + chr7_3:16419044-16419063 Msa1094520:five_prime_UTR 45.0%
AAAGCAGTCGAAGGTCTATC+TGG - chr7_3:16419322-16419341 None:intergenic 45.0%
CGAACTTCACCACAACCAAT+TGG - chr7_3:16419418-16419437 None:intergenic 45.0%
!! TTTGGTCTGAGTTCTGAGAG+AGG + chr7_3:16419479-16419498 Msa1094520:five_prime_UTR 45.0%
! TTCGAGTAGGTTGGCAAAGA+CGG + chr7_3:16419632-16419651 Msa1094520:five_prime_UTR 45.0%
GCAAAGACGGTGGATAAGAA+TGG + chr7_3:16419645-16419664 Msa1094520:five_prime_UTR 45.0%
!!! GCCGGATCTTGTTTTGATTG+AGG + chr7_3:16419873-16419892 Msa1094520:CDS 45.0%
ATCATCTGCTATATCAGGCC+GGG - chr7_3:16420097-16420116 None:intergenic 45.0%
AACGAAGGAAAAACTGGCGA+GGG - chr7_3:16420171-16420190 None:intergenic 45.0%
TAACGAAGGAAAAACTGGCG+AGG - chr7_3:16420172-16420191 None:intergenic 45.0%
TTGGGATCACAGTACAGCAT+AGG + chr7_3:16420208-16420227 Msa1094520:CDS 45.0%
TGCAGACATTTATGCAGGCA+AGG - chr7_3:16420399-16420418 None:intergenic 45.0%
! AAGTTAAGGCACCATCTGCA+TGG + chr7_3:16420449-16420468 Msa1094520:CDS 45.0%
!! GATATATGGTGGGCGATACA+TGG - chr7_3:16420774-16420793 None:intergenic 45.0%
GCTATTTGGGCGATATATGG+TGG - chr7_3:16420785-16420804 None:intergenic 45.0%
CGAAAGGCGAAGAGCTATTT+GGG - chr7_3:16420798-16420817 None:intergenic 45.0%
CCAAATAGCTCTTCGCCTTT+CGG + chr7_3:16420796-16420815 Msa1094520:intron 45.0%
CAAATAGCTCTTCGCCTTTC+GGG + chr7_3:16420797-16420816 Msa1094520:intron 45.0%
CTCTTCTAATGACTCCCGAA+AGG - chr7_3:16420814-16420833 None:intergenic 45.0%
!! TTCAGTTTGGTGTAGGAGGA+CGG + chr7_3:16421054-16421073 Msa1094520:intron 45.0%
GGATAGGTGGGAATCAAGAT+TGG + chr7_3:16421120-16421139 Msa1094520:CDS 45.0%
ACATATCAAATGGACGCCCT+TGG - chr7_3:16421156-16421175 None:intergenic 45.0%
TGTGGGGACAAAAAGAACCT+AGG - chr7_3:16421344-16421363 None:intergenic 45.0%
TGGCAAGGTTAGAAGCTACA+TGG - chr7_3:16421568-16421587 None:intergenic 45.0%
GCTATTGTCCTGAAGTAGGT+AGG - chr7_3:16421629-16421648 None:intergenic 45.0%
! AGAACGGTGGAGAGGTTGAT+CGG - chr7_3:16418793-16418812 None:intergenic 50.0%
AGTTTGAGAGAACGGTGGAG+AGG - chr7_3:16418801-16418820 None:intergenic 50.0%
CTCACTCACTACAGCGAGAA+CGG - chr7_3:16418988-16419007 None:intergenic 50.0%
CATTAAAGCCCCTGCCTTGA+AGG - chr7_3:16419255-16419274 None:intergenic 50.0%
! TGAAAAGGCGCATTGTGTGC+CGG - chr7_3:16419906-16419925 None:intergenic 50.0%
GCGCCTTCTCACAAGTATTC+AGG + chr7_3:16419978-16419997 Msa1094520:CDS 50.0%
ACGAAGGAAAAACTGGCGAG+GGG - chr7_3:16420170-16420189 None:intergenic 50.0%
GCAGGCAAGGAAATCAGACA+TGG - chr7_3:16420386-16420405 None:intergenic 50.0%
GCACCATCTGCATGGAAAGA+GGG + chr7_3:16420457-16420476 Msa1094520:CDS 50.0%
ATGCCCTCTTTCCATGCAGA+TGG - chr7_3:16420463-16420482 None:intergenic 50.0%
! TGGTGGGCGATACATGGAAA+GGG - chr7_3:16420768-16420787 None:intergenic 50.0%
!! ATGGTGGGCGATACATGGAA+AGG - chr7_3:16420769-16420788 None:intergenic 50.0%
CCGAAAGGCGAAGAGCTATT+TGG - chr7_3:16420799-16420818 None:intergenic 50.0%
AGGACGGATGAACTTGTCGA+TGG + chr7_3:16421070-16421089 Msa1094520:CDS 50.0%
! AAAGAACCTAGGCTGGTTGC+AGG - chr7_3:16421333-16421352 None:intergenic 50.0%
GGGACAAAAAGAACCTAGGC+TGG - chr7_3:16421340-16421359 None:intergenic 50.0%
CCTGCATGCTATTGCGAAGA+CGG - chr7_3:16421734-16421753 None:intergenic 50.0%
GTGCGTGATGAACACAGACA+GGG + chr7_3:16421817-16421836 Msa1094520:three_prime_UTR 50.0%
TGATGAACACAGACAGGGAC+TGG + chr7_3:16421822-16421841 Msa1094520:three_prime_UTR 50.0%
AAGGGGGAGTGAGAGTTGAG+GGG - chr7_3:16418713-16418732 None:intergenic 55.0%
GAAGGGGGAGTGAGAGTTGA+GGG - chr7_3:16418714-16418733 None:intergenic 55.0%
AGAAGGGGGAGTGAGAGTTG+AGG - chr7_3:16418715-16418734 None:intergenic 55.0%
TCGAGAGGAAGATACGGCAG+CGG - chr7_3:16419084-16419103 None:intergenic 55.0%
! CAAGGCAGGGGCTTTAATGC+TGG + chr7_3:16419256-16419275 Msa1094520:intron 55.0%
! GAGTAGGTTGGCAAAGACGG+TGG + chr7_3:16419635-16419654 Msa1094520:five_prime_UTR 55.0%
TGCTGCAGGAATTCACAGCC+CGG + chr7_3:16420076-16420095 Msa1094520:intron 55.0%
GGCGAGGGGATAAACAACTG+AGG - chr7_3:16420156-16420175 None:intergenic 55.0%
GGCACCATCTGCATGGAAAG+AGG + chr7_3:16420456-16420475 Msa1094520:CDS 55.0%
GAACTTCCTGCAACCAGCCT+AGG + chr7_3:16421324-16421343 Msa1094520:intron 55.0%
CCTGAAGTAGGTAGGCACGT+TGG - chr7_3:16421621-16421640 None:intergenic 55.0%
CCAACGTGCCTACCTACTTC+AGG + chr7_3:16421618-16421637 Msa1094520:intron 55.0%
CCGTCTTCGCAATAGCATGC+AGG + chr7_3:16421731-16421750 Msa1094520:intron 55.0%
GGTGCGTGATGAACACAGAC+AGG + chr7_3:16421816-16421835 Msa1094520:three_prime_UTR 55.0%
AGACAAAAATCGGCGGCGGG+AGG - chr7_3:16418865-16418884 None:intergenic 60.0%
! TGAGAAGGCGCGTTGTGTGC+CGG - chr7_3:16419969-16419988 None:intergenic 60.0%
CAAAAATCGGCGGCGGGAGG+TGG - chr7_3:16418862-16418881 None:intergenic 65.0%
AATCGGCGGCGGGAGGTGGT+TGG - chr7_3:16418858-16418877 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 16418676 16421972 16418676 ID=Msa1094520;Name=Msa1094520
chr7_3 mRNA 16418676 16421972 16418676 ID=Msa1094520-mRNA-1;Parent=Msa1094520;Name=Msa1094520-mRNA-1;_AED=0.04;_eAED=0.04;_QI=1072|0.6|0.83|0.83|0.4|0.5|6|179|173
chr7_3 exon 16418676 16419155 16418676 ID=Msa1094520-mRNA-1:exon:3656;Parent=Msa1094520-mRNA-1
chr7_3 exon 16419264 16419999 16419264 ID=Msa1094520-mRNA-1:exon:3657;Parent=Msa1094520-mRNA-1
chr7_3 exon 16420084 16420229 16420084 ID=Msa1094520-mRNA-1:exon:3658;Parent=Msa1094520-mRNA-1
chr7_3 exon 16420436 16420482 16420436 ID=Msa1094520-mRNA-1:exon:3659;Parent=Msa1094520-mRNA-1
chr7_3 exon 16421059 16421225 16421059 ID=Msa1094520-mRNA-1:exon:3660;Parent=Msa1094520-mRNA-1
chr7_3 exon 16421776 16421972 16421776 ID=Msa1094520-mRNA-1:exon:3661;Parent=Msa1094520-mRNA-1
chr7_3 five_prime_UTR 16418676 16419155 16418676 ID=Msa1094520-mRNA-1:five_prime_utr;Parent=Msa1094520-mRNA-1
chr7_3 five_prime_UTR 16419264 16419855 16419264 ID=Msa1094520-mRNA-1:five_prime_utr;Parent=Msa1094520-mRNA-1
chr7_3 CDS 16419856 16419999 16419856 ID=Msa1094520-mRNA-1:cds;Parent=Msa1094520-mRNA-1
chr7_3 CDS 16420084 16420229 16420084 ID=Msa1094520-mRNA-1:cds;Parent=Msa1094520-mRNA-1
chr7_3 CDS 16420436 16420482 16420436 ID=Msa1094520-mRNA-1:cds;Parent=Msa1094520-mRNA-1
chr7_3 CDS 16421059 16421225 16421059 ID=Msa1094520-mRNA-1:cds;Parent=Msa1094520-mRNA-1
chr7_3 CDS 16421776 16421793 16421776 ID=Msa1094520-mRNA-1:cds;Parent=Msa1094520-mRNA-1
chr7_3 three_prime_UTR 16421794 16421972 16421794 ID=Msa1094520-mRNA-1:three_prime_utr;Parent=Msa1094520-mRNA-1
Gene Sequence

>Msa1094520

ATGTTCTTGTTTCTTTGGCCGGATCTTGTTTTGATTGAGGCGCTTTTCCGGCACACAATGCGCCTTTTCACAACTATATCCTTCATATCTGTTTTGATTGAGGCGCTTTTCCGGCACACAACGCGCCTTCTCACAAGTATTCAGGAATTCACAGCCCGGCCTGATATAGCAGATGATTGTTTTTTGTTGGCTTCAAGGTGTATTCGCTACTGTCCTCAGTTGTTTATCCCCTCGCCAGTTTTTCCTTCGTTAGTTGATTGCTCTATGATTGGGATCACAGTACAGCATAGGCTCACTGTCTATAAGTTAAGGCACCATCTGCATGGAAAGAGGGCATTTTGGTGTAGGAGGACGGATGAACTTGTCGATGGCCCTAATGCTTCACATATTACAGCAATGGATAGGTGGGAATCAAGATTGGACGAACTTTTCCAAGGGCGTCCATTTGATATGTTTGATGCTGCTTTATCAGATACCGTCAACAGATTTCCTGTTGATATCCAGGATTTTGGTTCTATGTGA
Protein sequence

>Msa1094520

MFLFLWPDLVLIEALFRHTMRLFTTISFISVLIEALFRHTTRLLTSIQEFTARPDIADDCFLLASRCIRYCPQLFIPSPVFPSLVDCSMIGITVQHRLTVYKLRHHLHGKRAFWCRRTDELVDGPNASHITAMDRWESRLDELFQGRPFDMFDAALSDTVNRFPVDIQDFGSM*