AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0680210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0680210 MtrunA17_Chr4g0026241 94.634 205 7 1 1 201 1 205 6.45e-142 394
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0680210 AT3G44380.1 67.598 179 58 0 5 183 6 184 2.99e-83 245

Find 70 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACTCTCACTTTAAACTTA+TGG 0.256093 4_4:-39103948 None:intergenic
AGGTGGAATGTTGGGTCTTT+TGG 0.258820 4_4:-39103589 None:intergenic
GTGATTGTGTTGTTATTATA+TGG 0.305249 4_4:-39103323 None:intergenic
ACGGAGAAAGGATGAAATTA+AGG 0.350413 4_4:-39103222 None:intergenic
GGAATGATTGTATTGGAAAA+AGG 0.350913 4_4:-39103261 None:intergenic
ACTGCACAACTTGAACTCTT+TGG 0.363523 4_4:+39104024 Msa0680210:CDS
TTATTATATACTTGATTAAG+TGG 0.386392 4_4:+39105142 Msa0680210:three_prime_UTR
AGAACTTCTGCGTCTACAAC+GGG 0.388729 4_4:-39103649 None:intergenic
ATATTGCAAACGCATCATTC+TGG 0.391996 4_4:-39104991 None:intergenic
GATGCGGCTGTTGATATTGC+TGG 0.402057 4_4:+39103901 Msa0680210:CDS
TAGTTGATTGGGGCTATGTT+TGG 0.404363 4_4:-39103694 None:intergenic
GTTGTTGATGAGTAGTTGAT+TGG 0.409024 4_4:-39103706 None:intergenic
CGGCTAGACTTAAAGCTCTT+CGG 0.412589 4_4:+39103821 Msa0680210:CDS
GTTCAGCTCCGGTCCAAGCC+GGG 0.439864 4_4:+39103761 Msa0680210:CDS
TTCTACGAAGGTTCTCTCCT+CGG 0.440988 4_4:+39103739 Msa0680210:CDS
AGCTGGACATCAGCAATAAT+AGG 0.444898 4_4:+39103523 Msa0680210:CDS
TTGAGATGAGGTGGAATGTT+GGG 0.453056 4_4:-39103597 None:intergenic
TTCTCTCCTCGGTTCAGCTC+CGG 0.454445 4_4:+39103750 Msa0680210:CDS
GGCTGAGACCCGGCTTGGAC+CGG 0.455944 4_4:-39103769 None:intergenic
GGTTCAGCTCCGGTCCAAGC+CGG 0.458364 4_4:+39103760 Msa0680210:CDS
TTGTTGATGAGTAGTTGATT+GGG 0.460242 4_4:-39103705 None:intergenic
TTATTATATGGTTATTATTG+TGG 0.461415 4_4:-39103311 None:intergenic
AGAAAAGAATGGACGGAGAA+AGG 0.463972 4_4:-39103234 None:intergenic
GATGTCTGACGTGGCGAAAA+GGG 0.470056 4_4:+39103867 Msa0680210:CDS
TGATGTCTGACGTGGCGAAA+AGG 0.476417 4_4:+39103866 Msa0680210:CDS
CAACTTGAACTCTTTGGCTC+AGG 0.480208 4_4:+39104030 Msa0680210:CDS
AATTAAGGATAATGCGTTTG+AGG 0.485940 4_4:-39103207 None:intergenic
TCGGTGGCTGAGACCCGGCT+TGG 0.489322 4_4:-39103774 None:intergenic
GAAGACATGAGTTTGGTGTT+TGG 0.492448 4_4:-39103396 None:intergenic
GGCAAAGGGAATGATTGTAT+TGG 0.495624 4_4:-39103268 None:intergenic
GCGTCTACAACGGGGAGGCT+TGG 0.496927 4_4:-39103640 None:intergenic
AGAATAACACGTGCAGTTCG+TGG 0.498573 4_4:-39103175 None:intergenic
GATTGGGGCTATGTTTGGAT+TGG 0.499436 4_4:-39103689 None:intergenic
AAAAGAGGTCACAATGGAGC+TGG 0.519825 4_4:+39103506 Msa0680210:CDS
TTCCCTTTGCCTCTCATAGT+CGG 0.523144 4_4:+39103280 Msa0680210:five_prime_UTR
CGAAGAGCTTTAAGTCTAGC+CGG 0.524961 4_4:-39103820 None:intergenic
AGGAGGAAGAAAAGAATGGA+CGG 0.526071 4_4:-39103241 None:intergenic
GTGCTAGGAAGACATGAGTT+TGG 0.534827 4_4:-39103403 None:intergenic
TGAAGTTGAAGCGGAAGCTG+AGG 0.537462 4_4:+39104963 Msa0680210:CDS
ATGTCAGTTACTGAGACTTC+CGG 0.539227 4_4:+39103801 Msa0680210:CDS
ACATCTCAGCTCCTCAGAGC+CGG 0.540190 4_4:+39103433 Msa0680210:five_prime_UTR
TGTTGATGAGTAGTTGATTG+GGG 0.546694 4_4:-39103704 None:intergenic
ATTGAGATGAGGTGGAATGT+TGG 0.554077 4_4:-39103598 None:intergenic
GCACCGACTATGAGAGGCAA+AGG 0.556990 4_4:-39103283 None:intergenic
GCCTCTCATAGTCGGTGCAA+TGG 0.558028 4_4:+39103288 Msa0680210:five_prime_UTR
TGAAGTTGATTGAGATGAGG+TGG 0.561080 4_4:-39103606 None:intergenic
GTGTTAACCCTCCGGCTCTG+AGG 0.578423 4_4:-39103444 None:intergenic
TGACGTGGCGAAAAGGGAGA+TGG 0.581747 4_4:+39103873 Msa0680210:CDS
TCTCAGCTCCTCAGAGCCGG+AGG 0.585891 4_4:+39103436 Msa0680210:five_prime_UTR
TGAGATGTATCTGAAGTGCT+AGG 0.586842 4_4:-39103418 None:intergenic
GCCATTGCACCGACTATGAG+AGG 0.594317 4_4:-39103289 None:intergenic
GAGAACTTCTGCGTCTACAA+CGG 0.599919 4_4:-39103650 None:intergenic
TGACCTCGGTGGCTGAGACC+CGG 0.603018 4_4:-39103779 None:intergenic
GTATGAGATTGTGATGGATG+TGG 0.605181 4_4:-39103362 None:intergenic
CGAGTAGATGATGAGTTGGC+AGG 0.605200 4_4:+39104934 Msa0680210:intron
CACCGACTATGAGAGGCAAA+GGG 0.606633 4_4:-39103282 None:intergenic
AGGTGAAGATGAAGTTGAAG+CGG 0.628392 4_4:+39104954 Msa0680210:CDS
TATGCGAGTAGATGATGAGT+TGG 0.630993 4_4:+39104930 Msa0680210:intron
AAGGGAGATGGTTCTTGATG+CGG 0.631762 4_4:+39103885 Msa0680210:CDS
CTCAGTAACTGACATGACCT+CGG 0.639739 4_4:-39103793 None:intergenic
CTCAGCTCCTCAGAGCCGGA+GGG 0.639933 4_4:+39103437 Msa0680210:five_prime_UTR
AATTGAGTATGAGATTGTGA+TGG 0.645677 4_4:-39103368 None:intergenic
CTTGGACCGGAGCTGAACCG+AGG 0.649536 4_4:-39103756 None:intergenic
AAGTGAAGTTGATTGAGATG+AGG 0.676539 4_4:-39103609 None:intergenic
ACCTTCGTAGAAAATTGACA+TGG 0.695121 4_4:-39103728 None:intergenic
GAGTCGAGTGATGTCTGACG+TGG 0.716777 4_4:+39103858 Msa0680210:CDS
AAGCCGGGTCTCAGCCACCG+AGG 0.724149 4_4:+39103776 Msa0680210:CDS
CTTCTGCGTCTACAACGGGG+AGG 0.732058 4_4:-39103645 None:intergenic
AGTAACTGACATGACCTCGG+TGG 0.742599 4_4:-39103790 None:intergenic
GAACTTCTGCGTCTACAACG+GGG 0.764589 4_4:-39103648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATGGTTATTATTG+TGG - chr4_4:39103314-39103333 None:intergenic 15.0%
!!! ATTTTAATTTGAGATGTTTT+AGG + chr4_4:39105059-39105078 Msa0680210:three_prime_UTR 15.0%
!! TTATTATATACTTGATTAAG+TGG + chr4_4:39105142-39105161 Msa0680210:three_prime_UTR 15.0%
!! TTATTATATGGTTATTATTG+TGG - chr4_4:39103314-39103333 None:intergenic 15.0%
!!! ATTTTAATTTGAGATGTTTT+AGG + chr4_4:39105059-39105078 Msa0680210:three_prime_UTR 15.0%
!! TTATTATATACTTGATTAAG+TGG + chr4_4:39105142-39105161 Msa0680210:three_prime_UTR 15.0%
!! CGAAATAATCAAACAAATTA+TGG - chr4_4:39104329-39104348 None:intergenic 20.0%
!! ATGTAATTAGTTGTTACTAA+TGG + chr4_4:39104439-39104458 Msa0680210:intron 20.0%
!! TGTAATTAGTTGTTACTAAT+GGG + chr4_4:39104440-39104459 Msa0680210:intron 20.0%
!! GTAAAAAATATTCTTCATCA+TGG - chr4_4:39104515-39104534 None:intergenic 20.0%
!! CGAAATAATCAAACAAATTA+TGG - chr4_4:39104329-39104348 None:intergenic 20.0%
!! ATGTAATTAGTTGTTACTAA+TGG + chr4_4:39104439-39104458 Msa0680210:intron 20.0%
!! TGTAATTAGTTGTTACTAAT+GGG + chr4_4:39104440-39104459 Msa0680210:intron 20.0%
!! GTAAAAAATATTCTTCATCA+TGG - chr4_4:39104515-39104534 None:intergenic 20.0%
!! GTGATTGTGTTGTTATTATA+TGG - chr4_4:39103326-39103345 None:intergenic 25.0%
! AAAAAACTACTCTCTCTTAA+TGG + chr4_4:39103477-39103496 Msa0680210:exon 25.0%
! TAACTCTCACTTTAAACTTA+TGG - chr4_4:39103951-39103970 None:intergenic 25.0%
! TCAATAACATCAAGAAAAAC+AGG - chr4_4:39103994-39104013 None:intergenic 25.0%
!! TTTTCTTGATGTTATTGATC+AGG + chr4_4:39103996-39104015 Msa0680210:CDS 25.0%
! CAAATTAAGCATAGAAAAAG+TGG + chr4_4:39104088-39104107 Msa0680210:intron 25.0%
! AGCACAAAACATTAAAAACA+GGG - chr4_4:39104189-39104208 None:intergenic 25.0%
! AAGCACAAAACATTAAAAAC+AGG - chr4_4:39104190-39104209 None:intergenic 25.0%
! TTACATGTGTATTTATACGT+AGG + chr4_4:39104531-39104550 Msa0680210:intron 25.0%
! TTTATACGTAGGATGTATAT+TGG + chr4_4:39104542-39104561 Msa0680210:intron 25.0%
!! GTGATTGTGTTGTTATTATA+TGG - chr4_4:39103326-39103345 None:intergenic 25.0%
! AAAAAACTACTCTCTCTTAA+TGG + chr4_4:39103477-39103496 Msa0680210:exon 25.0%
! TAACTCTCACTTTAAACTTA+TGG - chr4_4:39103951-39103970 None:intergenic 25.0%
! TCAATAACATCAAGAAAAAC+AGG - chr4_4:39103994-39104013 None:intergenic 25.0%
!! TTTTCTTGATGTTATTGATC+AGG + chr4_4:39103996-39104015 Msa0680210:CDS 25.0%
! CAAATTAAGCATAGAAAAAG+TGG + chr4_4:39104088-39104107 Msa0680210:intron 25.0%
! AGCACAAAACATTAAAAACA+GGG - chr4_4:39104189-39104208 None:intergenic 25.0%
! AAGCACAAAACATTAAAAAC+AGG - chr4_4:39104190-39104209 None:intergenic 25.0%
! TTACATGTGTATTTATACGT+AGG + chr4_4:39104531-39104550 Msa0680210:intron 25.0%
! TTTATACGTAGGATGTATAT+TGG + chr4_4:39104542-39104561 Msa0680210:intron 25.0%
AATTAAGGATAATGCGTTTG+AGG - chr4_4:39103210-39103229 None:intergenic 30.0%
AAAAAGGAGGAAGAAAAGAA+TGG - chr4_4:39103248-39103267 None:intergenic 30.0%
ATGATTGTATTGGAAAAAGG+AGG - chr4_4:39103261-39103280 None:intergenic 30.0%
GGAATGATTGTATTGGAAAA+AGG - chr4_4:39103264-39103283 None:intergenic 30.0%
AATTGAGTATGAGATTGTGA+TGG - chr4_4:39103371-39103390 None:intergenic 30.0%
!!! TTTTTTTTGTGTTAACCCTC+CGG - chr4_4:39103455-39103474 None:intergenic 30.0%
TCTTAATGGAAGAGAAAAAG+AGG + chr4_4:39103491-39103510 Msa0680210:exon 30.0%
! TTGTTGATGAGTAGTTGATT+GGG - chr4_4:39103708-39103727 None:intergenic 30.0%
!! AAAAAGTGGTGTGATTTGTT+AGG + chr4_4:39104102-39104121 Msa0680210:intron 30.0%
! ATTTGTTAGGTTTGGGATAT+TGG + chr4_4:39104115-39104134 Msa0680210:intron 30.0%
AAAAATCAAAACGTGACGTT+AGG - chr4_4:39104148-39104167 None:intergenic 30.0%
TGTATACTATATAGAGTCGA+CGG + chr4_4:39104489-39104508 Msa0680210:intron 30.0%
AACTAAGTCGTGATTAATAC+GGG - chr4_4:39104722-39104741 None:intergenic 30.0%
CAACTAAGTCGTGATTAATA+CGG - chr4_4:39104723-39104742 None:intergenic 30.0%
! ACATTCATTTGTTTCTCATG+TGG + chr4_4:39104891-39104910 Msa0680210:intron 30.0%
AATTAAGGATAATGCGTTTG+AGG - chr4_4:39103210-39103229 None:intergenic 30.0%
AAAAAGGAGGAAGAAAAGAA+TGG - chr4_4:39103248-39103267 None:intergenic 30.0%
ATGATTGTATTGGAAAAAGG+AGG - chr4_4:39103261-39103280 None:intergenic 30.0%
GGAATGATTGTATTGGAAAA+AGG - chr4_4:39103264-39103283 None:intergenic 30.0%
AATTGAGTATGAGATTGTGA+TGG - chr4_4:39103371-39103390 None:intergenic 30.0%
!!! TTTTTTTTGTGTTAACCCTC+CGG - chr4_4:39103455-39103474 None:intergenic 30.0%
TCTTAATGGAAGAGAAAAAG+AGG + chr4_4:39103491-39103510 Msa0680210:exon 30.0%
! TTGTTGATGAGTAGTTGATT+GGG - chr4_4:39103708-39103727 None:intergenic 30.0%
!! AAAAAGTGGTGTGATTTGTT+AGG + chr4_4:39104102-39104121 Msa0680210:intron 30.0%
! ATTTGTTAGGTTTGGGATAT+TGG + chr4_4:39104115-39104134 Msa0680210:intron 30.0%
AAAAATCAAAACGTGACGTT+AGG - chr4_4:39104148-39104167 None:intergenic 30.0%
TGTATACTATATAGAGTCGA+CGG + chr4_4:39104489-39104508 Msa0680210:intron 30.0%
AACTAAGTCGTGATTAATAC+GGG - chr4_4:39104722-39104741 None:intergenic 30.0%
CAACTAAGTCGTGATTAATA+CGG - chr4_4:39104723-39104742 None:intergenic 30.0%
! ACATTCATTTGTTTCTCATG+TGG + chr4_4:39104891-39104910 Msa0680210:intron 30.0%
ACGGAGAAAGGATGAAATTA+AGG - chr4_4:39103225-39103244 None:intergenic 35.0%
AAGAGAAAAAGAGGTCACAA+TGG + chr4_4:39103500-39103519 Msa0680210:CDS 35.0%
! AAGTGAAGTTGATTGAGATG+AGG - chr4_4:39103612-39103631 None:intergenic 35.0%
! TGTTGATGAGTAGTTGATTG+GGG - chr4_4:39103707-39103726 None:intergenic 35.0%
! GTTGTTGATGAGTAGTTGAT+TGG - chr4_4:39103709-39103728 None:intergenic 35.0%
! ACCATGTCAATTTTCTACGA+AGG + chr4_4:39103727-39103746 Msa0680210:CDS 35.0%
ACCTTCGTAGAAAATTGACA+TGG - chr4_4:39103731-39103750 None:intergenic 35.0%
!! TGGTGTGATTTGTTAGGTTT+GGG + chr4_4:39104108-39104127 Msa0680210:intron 35.0%
GCTTCTCAAGTTTGAATTGT+AGG + chr4_4:39104206-39104225 Msa0680210:intron 35.0%
TAATGGGATGTTCTTAGTCA+AGG + chr4_4:39104456-39104475 Msa0680210:intron 35.0%
AGATCATCCTACAAATGTCA+AGG - chr4_4:39104606-39104625 None:intergenic 35.0%
CGTATTAATCACGACTTAGT+TGG + chr4_4:39104721-39104740 Msa0680210:intron 35.0%
! ATCGTACTCTGCTCATTTTA+TGG + chr4_4:39104850-39104869 Msa0680210:intron 35.0%
ATATTGCAAACGCATCATTC+TGG - chr4_4:39104994-39105013 None:intergenic 35.0%
ACGGAGAAAGGATGAAATTA+AGG - chr4_4:39103225-39103244 None:intergenic 35.0%
AAGAGAAAAAGAGGTCACAA+TGG + chr4_4:39103500-39103519 Msa0680210:CDS 35.0%
! AAGTGAAGTTGATTGAGATG+AGG - chr4_4:39103612-39103631 None:intergenic 35.0%
! TGTTGATGAGTAGTTGATTG+GGG - chr4_4:39103707-39103726 None:intergenic 35.0%
! GTTGTTGATGAGTAGTTGAT+TGG - chr4_4:39103709-39103728 None:intergenic 35.0%
! ACCATGTCAATTTTCTACGA+AGG + chr4_4:39103727-39103746 Msa0680210:CDS 35.0%
ACCTTCGTAGAAAATTGACA+TGG - chr4_4:39103731-39103750 None:intergenic 35.0%
!! TGGTGTGATTTGTTAGGTTT+GGG + chr4_4:39104108-39104127 Msa0680210:intron 35.0%
GCTTCTCAAGTTTGAATTGT+AGG + chr4_4:39104206-39104225 Msa0680210:intron 35.0%
TAATGGGATGTTCTTAGTCA+AGG + chr4_4:39104456-39104475 Msa0680210:intron 35.0%
AGATCATCCTACAAATGTCA+AGG - chr4_4:39104606-39104625 None:intergenic 35.0%
CGTATTAATCACGACTTAGT+TGG + chr4_4:39104721-39104740 Msa0680210:intron 35.0%
! ATCGTACTCTGCTCATTTTA+TGG + chr4_4:39104850-39104869 Msa0680210:intron 35.0%
ATATTGCAAACGCATCATTC+TGG - chr4_4:39104994-39105013 None:intergenic 35.0%
AGAAAAGAATGGACGGAGAA+AGG - chr4_4:39103237-39103256 None:intergenic 40.0%
AGGAGGAAGAAAAGAATGGA+CGG - chr4_4:39103244-39103263 None:intergenic 40.0%
GGCAAAGGGAATGATTGTAT+TGG - chr4_4:39103271-39103290 None:intergenic 40.0%
!! GTATGAGATTGTGATGGATG+TGG - chr4_4:39103365-39103384 None:intergenic 40.0%
!! GAAGACATGAGTTTGGTGTT+TGG - chr4_4:39103399-39103418 None:intergenic 40.0%
! TGAGATGTATCTGAAGTGCT+AGG - chr4_4:39103421-39103440 None:intergenic 40.0%
AGCTGGACATCAGCAATAAT+AGG + chr4_4:39103523-39103542 Msa0680210:CDS 40.0%
TTGAGATGAGGTGGAATGTT+GGG - chr4_4:39103600-39103619 None:intergenic 40.0%
ATTGAGATGAGGTGGAATGT+TGG - chr4_4:39103601-39103620 None:intergenic 40.0%
! TGAAGTTGATTGAGATGAGG+TGG - chr4_4:39103609-39103628 None:intergenic 40.0%
TAGTTGATTGGGGCTATGTT+TGG - chr4_4:39103697-39103716 None:intergenic 40.0%
ATGTCAGTTACTGAGACTTC+CGG + chr4_4:39103801-39103820 Msa0680210:CDS 40.0%
ACTGCACAACTTGAACTCTT+TGG + chr4_4:39104024-39104043 Msa0680210:CDS 40.0%
!! GTGGTGTGATTTGTTAGGTT+TGG + chr4_4:39104107-39104126 Msa0680210:intron 40.0%
GATCTTCTTCGATTCAATCG+CGG - chr4_4:39104576-39104595 None:intergenic 40.0%
TGAATCGAAGAAGATCGATG+CGG + chr4_4:39104580-39104599 Msa0680210:intron 40.0%
GTAAATGTAGCGTACCTTTG+AGG + chr4_4:39104629-39104648 Msa0680210:intron 40.0%
CATGAATCAGAGTTTCCTCA+AGG - chr4_4:39104678-39104697 None:intergenic 40.0%
TATGCGAGTAGATGATGAGT+TGG + chr4_4:39104930-39104949 Msa0680210:intron 40.0%
AGGTGAAGATGAAGTTGAAG+CGG + chr4_4:39104954-39104973 Msa0680210:CDS 40.0%
AGAAAAGAATGGACGGAGAA+AGG - chr4_4:39103237-39103256 None:intergenic 40.0%
AGGAGGAAGAAAAGAATGGA+CGG - chr4_4:39103244-39103263 None:intergenic 40.0%
GGCAAAGGGAATGATTGTAT+TGG - chr4_4:39103271-39103290 None:intergenic 40.0%
!! GTATGAGATTGTGATGGATG+TGG - chr4_4:39103365-39103384 None:intergenic 40.0%
!! GAAGACATGAGTTTGGTGTT+TGG - chr4_4:39103399-39103418 None:intergenic 40.0%
! TGAGATGTATCTGAAGTGCT+AGG - chr4_4:39103421-39103440 None:intergenic 40.0%
AGCTGGACATCAGCAATAAT+AGG + chr4_4:39103523-39103542 Msa0680210:CDS 40.0%
TTGAGATGAGGTGGAATGTT+GGG - chr4_4:39103600-39103619 None:intergenic 40.0%
ATTGAGATGAGGTGGAATGT+TGG - chr4_4:39103601-39103620 None:intergenic 40.0%
! TGAAGTTGATTGAGATGAGG+TGG - chr4_4:39103609-39103628 None:intergenic 40.0%
TAGTTGATTGGGGCTATGTT+TGG - chr4_4:39103697-39103716 None:intergenic 40.0%
ATGTCAGTTACTGAGACTTC+CGG + chr4_4:39103801-39103820 Msa0680210:CDS 40.0%
ACTGCACAACTTGAACTCTT+TGG + chr4_4:39104024-39104043 Msa0680210:CDS 40.0%
!! GTGGTGTGATTTGTTAGGTT+TGG + chr4_4:39104107-39104126 Msa0680210:intron 40.0%
GATCTTCTTCGATTCAATCG+CGG - chr4_4:39104576-39104595 None:intergenic 40.0%
TGAATCGAAGAAGATCGATG+CGG + chr4_4:39104580-39104599 Msa0680210:intron 40.0%
GTAAATGTAGCGTACCTTTG+AGG + chr4_4:39104629-39104648 Msa0680210:intron 40.0%
CATGAATCAGAGTTTCCTCA+AGG - chr4_4:39104678-39104697 None:intergenic 40.0%
TATGCGAGTAGATGATGAGT+TGG + chr4_4:39104930-39104949 Msa0680210:intron 40.0%
AGGTGAAGATGAAGTTGAAG+CGG + chr4_4:39104954-39104973 Msa0680210:CDS 40.0%
TTCCCTTTGCCTCTCATAGT+CGG + chr4_4:39103280-39103299 Msa0680210:five_prime_UTR 45.0%
GTGCTAGGAAGACATGAGTT+TGG - chr4_4:39103406-39103425 None:intergenic 45.0%
AAAAGAGGTCACAATGGAGC+TGG + chr4_4:39103506-39103525 Msa0680210:CDS 45.0%
! AGGTGGAATGTTGGGTCTTT+TGG - chr4_4:39103592-39103611 None:intergenic 45.0%
AGAACTTCTGCGTCTACAAC+GGG - chr4_4:39103652-39103671 None:intergenic 45.0%
GAGAACTTCTGCGTCTACAA+CGG - chr4_4:39103653-39103672 None:intergenic 45.0%
GATTGGGGCTATGTTTGGAT+TGG - chr4_4:39103692-39103711 None:intergenic 45.0%
TTCTACGAAGGTTCTCTCCT+CGG + chr4_4:39103739-39103758 Msa0680210:CDS 45.0%
CTCAGTAACTGACATGACCT+CGG - chr4_4:39103796-39103815 None:intergenic 45.0%
!! CGAAGAGCTTTAAGTCTAGC+CGG - chr4_4:39103823-39103842 None:intergenic 45.0%
CGGCTAGACTTAAAGCTCTT+CGG + chr4_4:39103821-39103840 Msa0680210:CDS 45.0%
AAGGGAGATGGTTCTTGATG+CGG + chr4_4:39103885-39103904 Msa0680210:CDS 45.0%
!! CACAGCTAGAGTTTTGTGGT+GGG + chr4_4:39103924-39103943 Msa0680210:CDS 45.0%
! CAACTTGAACTCTTTGGCTC+AGG + chr4_4:39104030-39104049 Msa0680210:CDS 45.0%
! TTGAACTCTTTGGCTCAGGT+CGG + chr4_4:39104034-39104053 Msa0680210:intron 45.0%
CTGGATATTGAGTGACCTTG+AGG + chr4_4:39104660-39104679 Msa0680210:intron 45.0%
TTCCCTTTGCCTCTCATAGT+CGG + chr4_4:39103280-39103299 Msa0680210:five_prime_UTR 45.0%
GTGCTAGGAAGACATGAGTT+TGG - chr4_4:39103406-39103425 None:intergenic 45.0%
AAAAGAGGTCACAATGGAGC+TGG + chr4_4:39103506-39103525 Msa0680210:CDS 45.0%
! AGGTGGAATGTTGGGTCTTT+TGG - chr4_4:39103592-39103611 None:intergenic 45.0%
AGAACTTCTGCGTCTACAAC+GGG - chr4_4:39103652-39103671 None:intergenic 45.0%
GAGAACTTCTGCGTCTACAA+CGG - chr4_4:39103653-39103672 None:intergenic 45.0%
GATTGGGGCTATGTTTGGAT+TGG - chr4_4:39103692-39103711 None:intergenic 45.0%
TTCTACGAAGGTTCTCTCCT+CGG + chr4_4:39103739-39103758 Msa0680210:CDS 45.0%
CTCAGTAACTGACATGACCT+CGG - chr4_4:39103796-39103815 None:intergenic 45.0%
!! CGAAGAGCTTTAAGTCTAGC+CGG - chr4_4:39103823-39103842 None:intergenic 45.0%
CGGCTAGACTTAAAGCTCTT+CGG + chr4_4:39103821-39103840 Msa0680210:CDS 45.0%
AAGGGAGATGGTTCTTGATG+CGG + chr4_4:39103885-39103904 Msa0680210:CDS 45.0%
!! CACAGCTAGAGTTTTGTGGT+GGG + chr4_4:39103924-39103943 Msa0680210:CDS 45.0%
! CAACTTGAACTCTTTGGCTC+AGG + chr4_4:39104030-39104049 Msa0680210:CDS 45.0%
! TTGAACTCTTTGGCTCAGGT+CGG + chr4_4:39104034-39104053 Msa0680210:intron 45.0%
CTGGATATTGAGTGACCTTG+AGG + chr4_4:39104660-39104679 Msa0680210:intron 45.0%
! CACCGACTATGAGAGGCAAA+GGG - chr4_4:39103285-39103304 None:intergenic 50.0%
!!! AACGGGGAGGCTTGGTTTTA+AGG - chr4_4:39103635-39103654 None:intergenic 50.0%
GAACTTCTGCGTCTACAACG+GGG - chr4_4:39103651-39103670 None:intergenic 50.0%
AGTAACTGACATGACCTCGG+TGG - chr4_4:39103793-39103812 None:intergenic 50.0%
TGATGTCTGACGTGGCGAAA+AGG + chr4_4:39103866-39103885 Msa0680210:CDS 50.0%
GATGTCTGACGTGGCGAAAA+GGG + chr4_4:39103867-39103886 Msa0680210:CDS 50.0%
GATGCGGCTGTTGATATTGC+TGG + chr4_4:39103901-39103920 Msa0680210:CDS 50.0%
! CTGGCACAGCTAGAGTTTTG+TGG + chr4_4:39103920-39103939 Msa0680210:CDS 50.0%
!! GCACAGCTAGAGTTTTGTGG+TGG + chr4_4:39103923-39103942 Msa0680210:CDS 50.0%
GATGCGGCCTTGACATTTGT+AGG + chr4_4:39104596-39104615 Msa0680210:intron 50.0%
!! AGCGTACCTTTGAGGCGTTT+CGG + chr4_4:39104637-39104656 Msa0680210:intron 50.0%
! CGAGTAGATGATGAGTTGGC+AGG + chr4_4:39104934-39104953 Msa0680210:intron 50.0%
TGAAGTTGAAGCGGAAGCTG+AGG + chr4_4:39104963-39104982 Msa0680210:CDS 50.0%
! CACCGACTATGAGAGGCAAA+GGG - chr4_4:39103285-39103304 None:intergenic 50.0%
!!! AACGGGGAGGCTTGGTTTTA+AGG - chr4_4:39103635-39103654 None:intergenic 50.0%
GAACTTCTGCGTCTACAACG+GGG - chr4_4:39103651-39103670 None:intergenic 50.0%
AGTAACTGACATGACCTCGG+TGG - chr4_4:39103793-39103812 None:intergenic 50.0%
TGATGTCTGACGTGGCGAAA+AGG + chr4_4:39103866-39103885 Msa0680210:CDS 50.0%
GATGTCTGACGTGGCGAAAA+GGG + chr4_4:39103867-39103886 Msa0680210:CDS 50.0%
GATGCGGCTGTTGATATTGC+TGG + chr4_4:39103901-39103920 Msa0680210:CDS 50.0%
! CTGGCACAGCTAGAGTTTTG+TGG + chr4_4:39103920-39103939 Msa0680210:CDS 50.0%
!! GCACAGCTAGAGTTTTGTGG+TGG + chr4_4:39103923-39103942 Msa0680210:CDS 50.0%
GATGCGGCCTTGACATTTGT+AGG + chr4_4:39104596-39104615 Msa0680210:intron 50.0%
!! AGCGTACCTTTGAGGCGTTT+CGG + chr4_4:39104637-39104656 Msa0680210:intron 50.0%
! CGAGTAGATGATGAGTTGGC+AGG + chr4_4:39104934-39104953 Msa0680210:intron 50.0%
TGAAGTTGAAGCGGAAGCTG+AGG + chr4_4:39104963-39104982 Msa0680210:CDS 50.0%
!! GCACCGACTATGAGAGGCAA+AGG - chr4_4:39103286-39103305 None:intergenic 55.0%
GCCTCTCATAGTCGGTGCAA+TGG + chr4_4:39103288-39103307 Msa0680210:five_prime_UTR 55.0%
!! GCCATTGCACCGACTATGAG+AGG - chr4_4:39103292-39103311 None:intergenic 55.0%
ACATCTCAGCTCCTCAGAGC+CGG + chr4_4:39103433-39103452 Msa0680210:five_prime_UTR 55.0%
TTCTCTCCTCGGTTCAGCTC+CGG + chr4_4:39103750-39103769 Msa0680210:CDS 55.0%
GAGTCGAGTGATGTCTGACG+TGG + chr4_4:39103858-39103877 Msa0680210:CDS 55.0%
TGACGTGGCGAAAAGGGAGA+TGG + chr4_4:39103873-39103892 Msa0680210:CDS 55.0%
!! TACCTTTGAGGCGTTTCGGC+TGG + chr4_4:39104641-39104660 Msa0680210:intron 55.0%
ATCCAGCCGAAACGCCTCAA+AGG - chr4_4:39104646-39104665 None:intergenic 55.0%
!! GCACCGACTATGAGAGGCAA+AGG - chr4_4:39103286-39103305 None:intergenic 55.0%
GCCTCTCATAGTCGGTGCAA+TGG + chr4_4:39103288-39103307 Msa0680210:five_prime_UTR 55.0%
!! GCCATTGCACCGACTATGAG+AGG - chr4_4:39103292-39103311 None:intergenic 55.0%
ACATCTCAGCTCCTCAGAGC+CGG + chr4_4:39103433-39103452 Msa0680210:five_prime_UTR 55.0%
TTCTCTCCTCGGTTCAGCTC+CGG + chr4_4:39103750-39103769 Msa0680210:CDS 55.0%
GAGTCGAGTGATGTCTGACG+TGG + chr4_4:39103858-39103877 Msa0680210:CDS 55.0%
TGACGTGGCGAAAAGGGAGA+TGG + chr4_4:39103873-39103892 Msa0680210:CDS 55.0%
!! TACCTTTGAGGCGTTTCGGC+TGG + chr4_4:39104641-39104660 Msa0680210:intron 55.0%
ATCCAGCCGAAACGCCTCAA+AGG - chr4_4:39104646-39104665 None:intergenic 55.0%
GTGTTAACCCTCCGGCTCTG+AGG - chr4_4:39103447-39103466 None:intergenic 60.0%
CTTCTGCGTCTACAACGGGG+AGG - chr4_4:39103648-39103667 None:intergenic 60.0%
GTGTTAACCCTCCGGCTCTG+AGG - chr4_4:39103447-39103466 None:intergenic 60.0%
CTTCTGCGTCTACAACGGGG+AGG - chr4_4:39103648-39103667 None:intergenic 60.0%
TCTCAGCTCCTCAGAGCCGG+AGG + chr4_4:39103436-39103455 Msa0680210:five_prime_UTR 65.0%
CTCAGCTCCTCAGAGCCGGA+GGG + chr4_4:39103437-39103456 Msa0680210:five_prime_UTR 65.0%
GCGTCTACAACGGGGAGGCT+TGG - chr4_4:39103643-39103662 None:intergenic 65.0%
CTTGGACCGGAGCTGAACCG+AGG - chr4_4:39103759-39103778 None:intergenic 65.0%
GGTTCAGCTCCGGTCCAAGC+CGG + chr4_4:39103760-39103779 Msa0680210:CDS 65.0%
GTTCAGCTCCGGTCCAAGCC+GGG + chr4_4:39103761-39103780 Msa0680210:CDS 65.0%
TGACCTCGGTGGCTGAGACC+CGG - chr4_4:39103782-39103801 None:intergenic 65.0%
TCTCAGCTCCTCAGAGCCGG+AGG + chr4_4:39103436-39103455 Msa0680210:five_prime_UTR 65.0%
CTCAGCTCCTCAGAGCCGGA+GGG + chr4_4:39103437-39103456 Msa0680210:five_prime_UTR 65.0%
GCGTCTACAACGGGGAGGCT+TGG - chr4_4:39103643-39103662 None:intergenic 65.0%
CTTGGACCGGAGCTGAACCG+AGG - chr4_4:39103759-39103778 None:intergenic 65.0%
GGTTCAGCTCCGGTCCAAGC+CGG + chr4_4:39103760-39103779 Msa0680210:CDS 65.0%
GTTCAGCTCCGGTCCAAGCC+GGG + chr4_4:39103761-39103780 Msa0680210:CDS 65.0%
TGACCTCGGTGGCTGAGACC+CGG - chr4_4:39103782-39103801 None:intergenic 65.0%
!! GGCTGAGACCCGGCTTGGAC+CGG - chr4_4:39103772-39103791 None:intergenic 70.0%
TCGGTGGCTGAGACCCGGCT+TGG - chr4_4:39103777-39103796 None:intergenic 70.0%
AAGCCGGGTCTCAGCCACCG+AGG + chr4_4:39103776-39103795 Msa0680210:CDS 70.0%
!! GGCTGAGACCCGGCTTGGAC+CGG - chr4_4:39103772-39103791 None:intergenic 70.0%
TCGGTGGCTGAGACCCGGCT+TGG - chr4_4:39103777-39103796 None:intergenic 70.0%
AAGCCGGGTCTCAGCCACCG+AGG + chr4_4:39103776-39103795 Msa0680210:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 39103176 39105191 39103176 ID=Msa0680210;Name=Msa0680210
chr4_4 mRNA 39103176 39105191 39103176 ID=Msa0680210-mRNA-1;Parent=Msa0680210;Name=Msa0680210-mRNA-1;_AED=0.00;_eAED=0.00;_QI=320|1|1|1|0|0.5|2|192|204
chr4_4 exon 39103176 39104051 39103176 ID=Msa0680210-mRNA-1:exon:9203;Parent=Msa0680210-mRNA-1
chr4_4 exon 39104941 39105191 39104941 ID=Msa0680210-mRNA-1:exon:9204;Parent=Msa0680210-mRNA-1
chr4_4 five_prime_UTR 39103176 39103495 39103176 ID=Msa0680210-mRNA-1:five_prime_utr;Parent=Msa0680210-mRNA-1
chr4_4 CDS 39103496 39104051 39103496 ID=Msa0680210-mRNA-1:cds;Parent=Msa0680210-mRNA-1
chr4_4 CDS 39104941 39104999 39104941 ID=Msa0680210-mRNA-1:cds;Parent=Msa0680210-mRNA-1
chr4_4 three_prime_UTR 39105000 39105191 39105000 ID=Msa0680210-mRNA-1:three_prime_utr;Parent=Msa0680210-mRNA-1
Gene Sequence

>Msa0680210

ATGGAAGAGAAAAAGAGGTCACAATGGAGCTGGACATCAGCAATAATAGGAGCAACAACAGCACTAGCAGCAACATCAATTCTCTCAGCAAAACCAAAAGACCCAACATTCCACCTCATCTCAATCAACTTCACTTCCTTAAAACCAAGCCTCCCCGTTGTAGACGCAGAAGTTCTCTTAACAGTTCATGTCACCAATCCAAACATAGCCCCAATCAACTACTCATCAACAACCATGTCAATTTTCTACGAAGGTTCTCTCCTCGGTTCAGCTCCGGTCCAAGCCGGGTCTCAGCCACCGAGGTCATGTCAGTTACTGAGACTTCCGGCTAGACTTAAAGCTCTTCGGCTCGCGAAACACGCGAGTCGAGTGATGTCTGACGTGGCGAAAAGGGAGATGGTTCTTGATGCGGCTGTTGATATTGCTGGCACAGCTAGAGTTTTGTGGTGGGACCATAAGTTTAAAGTGAGAGTTAATAGTCATATTACTGTTGATCCTGTTTTTCTTGATGTTATTGATCAGGAGAATACTGCACAACTTGAACTCTTTGGCTCAGATGATGAGTTGGCAGGTGAAGATGAAGTTGAAGCGGAAGCTGAGGAGCAACCAGAATGA
Protein sequence

>Msa0680210

MEEKKRSQWSWTSAIIGATTALAATSILSAKPKDPTFHLISINFTSLKPSLPVVDAEVLLTVHVTNPNIAPINYSSTTMSIFYEGSLLGSAPVQAGSQPPRSCQLLRLPARLKALRLAKHASRVMSDVAKREMVLDAAVDIAGTARVLWWDHKFKVRVNSHITVDPVFLDVIDQENTAQLELFGSDDELAGEDEVEAEAEEQPE*