AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0692660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0692660 MtrunA17_Chr4g0075851 53.191 141 28 3 1 103 1 141 5.86e-38 129
Msa0692660 MtrunA17_Chr4g0075841 59.459 74 22 1 110 183 1 66 7.65e-20 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 107 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTCTTCGTGATAATTT+TGG 0.137277 4_4:+61304414 Msa0692660:three_prime_UTR
TTCACTAGAACAACCGTAAA+AGG 0.199901 4_4:-61304615 None:intergenic
TTTCATCAAATAAATGAAAT+TGG 0.204145 4_4:-61301842 None:intergenic
GACAACTCCACTTCCCTTCT+TGG 0.225568 4_4:-61303593 None:intergenic
CTCACTTGTTCATCTAAATA+TGG 0.252421 4_4:+61303695 Msa0692660:exon
ACCGTAAAAGGGGTCAAATT+TGG 0.254584 4_4:-61304603 None:intergenic
CTACGTTTCCCACTGATAAT+AGG 0.269442 4_4:-61302950 None:intergenic
TAGTGGAGTTGTCACTTCTA+AGG 0.313166 4_4:+61303630 Msa0692660:CDS
GTAATTTAGGACCGTTACTA+TGG 0.345244 4_4:+61302892 Msa0692660:intron
AATAAGCCAAACTTGTTTGT+TGG 0.361245 4_4:+61304058 Msa0692660:three_prime_UTR
CTTTGACCAACAAACAAGTT+TGG 0.361598 4_4:-61304064 None:intergenic
TACAAAACAGAGAAACAAAA+AGG 0.362184 4_4:-61301785 None:intergenic
CCGTAAAAGGGGTCAAATTT+GGG 0.374400 4_4:-61304602 None:intergenic
GTCAGACCCCTTTCCCAACT+TGG 0.382301 4_4:-61304032 None:intergenic
ATCGAAATGCTGATTTGATC+AGG 0.386364 4_4:+61303896 Msa0692660:three_prime_UTR
CAATTCCGATTGAAGCTTGC+CGG 0.387284 4_4:+61301911 Msa0692660:five_prime_UTR
TCAACTTTACTGTTTCAATC+TGG 0.387747 4_4:+61304376 Msa0692660:three_prime_UTR
AAAACTCAAAACTACTGTTA+TGG 0.398181 4_4:+61302979 Msa0692660:CDS
GCCTTAGTCCACCAATCCTT+GGG 0.401731 4_4:-61303781 None:intergenic
CGGCAAGCTTCAATCGGAAT+TGG 0.410141 4_4:-61301910 None:intergenic
AGCCTTAGTCCACCAATCCT+TGG 0.418257 4_4:-61303782 None:intergenic
CTCGTGAATTCAATTGTAGC+TGG 0.420262 4_4:+61303830 Msa0692660:three_prime_UTR
GAAGGAGAAGGAGAAAGAAA+AGG 0.425977 4_4:+61304305 Msa0692660:three_prime_UTR
GCAACAGCTAAAGGCTTTGC+TGG 0.426278 4_4:+61304209 Msa0692660:intron
ATGAAAGAGATAAGGAAGTC+AGG 0.429458 4_4:+61303419 Msa0692660:CDS
ATGCAAAGGGACGCCAAGTT+GGG 0.434365 4_4:+61304019 Msa0692660:three_prime_UTR
TACAGTACAAATCAGTTACT+TGG 0.441479 4_4:-61304548 None:intergenic
CTGCATTGTCTGCAGTGTCC+TGG 0.444540 4_4:-61303057 None:intergenic
TTCTAAAGGTGATCATGTCC+TGG 0.454471 4_4:+61302922 Msa0692660:CDS
GTCAGGATAAATTCAAATCC+TGG 0.455998 4_4:+61303436 Msa0692660:CDS
TGATCCTTTCACTGAAGAGC+TGG 0.462276 4_4:+61303187 Msa0692660:CDS
AAAGAGAACTCAGACTCAGC+TGG 0.468170 4_4:-61301816 None:intergenic
TCACTAGAACAACCGTAAAA+GGG 0.471595 4_4:-61304614 None:intergenic
AATGACACCTGAGATGCTTA+AGG 0.475276 4_4:+61303298 Msa0692660:CDS
TCTACGAATATGAATGTTGA+AGG 0.476392 4_4:-61302007 None:intergenic
GATGCAAAGGGACGCCAAGT+TGG 0.477890 4_4:+61304018 Msa0692660:three_prime_UTR
AGGGACGCCAAGTTGGGAAA+GGG 0.486300 4_4:+61304025 Msa0692660:three_prime_UTR
CTCAAAACTACTGTTATGGA+TGG 0.487863 4_4:+61302983 Msa0692660:CDS
AAGTGGAGTTGTCACTCCTA+AGG 0.488445 4_4:+61303603 Msa0692660:CDS
TCCCAAGGATTGGTGGACTA+AGG 0.492077 4_4:+61303780 Msa0692660:three_prime_UTR
GAATTCAATTGTAGCTGGGC+AGG 0.494104 4_4:+61303835 Msa0692660:three_prime_UTR
AGAAAAGGAAAAGTAGCAGC+AGG 0.496648 4_4:+61304320 Msa0692660:three_prime_UTR
CAGCTAAAGGCTTTGCTGGA+TGG 0.497414 4_4:+61304213 Msa0692660:intron
GCAAGGGAAGTCACATATCC+AGG 0.497708 4_4:+61303039 Msa0692660:CDS
GTCTCCTCCAAGAAGGGAAG+TGG 0.499665 4_4:+61303586 Msa0692660:CDS
ACATGGACAATTGATGCAAA+GGG 0.502711 4_4:+61304006 Msa0692660:three_prime_UTR
AAGGGACGCCAAGTTGGGAA+AGG 0.502741 4_4:+61304024 Msa0692660:three_prime_UTR
AGTGGAGTTGTCACTTCTAA+GGG 0.511110 4_4:+61303631 Msa0692660:CDS
GTCATCAGAATTATTCATAG+TGG 0.511621 4_4:-61304089 None:intergenic
GGAGATGGATGAAAGAGATA+AGG 0.513093 4_4:+61303411 Msa0692660:CDS
AGGGAAGTGGTAGTAGGCAA+GGG 0.515942 4_4:+61303023 Msa0692660:CDS
GAGGGAAGTGGTAGTAGGCA+AGG 0.518111 4_4:+61303022 Msa0692660:CDS
AACTCCACTTCCCTTCTTGG+AGG 0.521516 4_4:-61303590 None:intergenic
TTTAGGACCGTTACTATGGA+GGG 0.521731 4_4:+61302896 Msa0692660:intron
TCAAGGAGGGAAGTGGTAGT+AGG 0.524258 4_4:+61303017 Msa0692660:CDS
AGAGAAGAAGGAGAAGGAGA+AGG 0.525181 4_4:+61304293 Msa0692660:three_prime_UTR
TGGCACCGGCAAGCTTCAAT+CGG 0.525511 4_4:-61301916 None:intergenic
ACAACTCCACTAGCTCCCTT+AGG 0.528809 4_4:-61303619 None:intergenic
CTTTCTCTCATCGCTCTCAA+CGG 0.528933 4_4:+61302042 Msa0692660:five_prime_UTR
AACATGGACAATTGATGCAA+AGG 0.535332 4_4:+61304005 Msa0692660:three_prime_UTR
TTTACAAGAGAAGAAGGAGA+AGG 0.541855 4_4:+61304287 Msa0692660:three_prime_UTR
CTGGATGGTAACAAACTGAT+GGG 0.543990 4_4:+61304228 Msa0692660:three_prime_UTR
ACTATGGAGGGAAGTTCTAA+AGG 0.549995 4_4:+61302908 Msa0692660:exon
TGTTACAGATGTTGATGAGC+TGG 0.551297 4_4:-61301936 None:intergenic
TATATATGTAAGTAACTACT+TGG 0.552644 4_4:-61301966 None:intergenic
AGCTCCAGCTCTTCAGTGAA+AGG 0.552717 4_4:-61303191 None:intergenic
GAACTTGTCAAGGAGGGAAG+TGG 0.557621 4_4:+61303010 Msa0692660:CDS
GGGACGCCAAGTTGGGAAAG+GGG 0.563286 4_4:+61304026 Msa0692660:three_prime_UTR
AAGAGTAGTCTCCTCCAAGA+AGG 0.563577 4_4:+61303579 Msa0692660:CDS
TCTAAAGGTGATCATGTCCT+GGG 0.566442 4_4:+61302923 Msa0692660:CDS
AGTGGAGTTGTCACTCCTAA+GGG 0.567341 4_4:+61303604 Msa0692660:CDS
GCTGGATGGTAACAAACTGA+TGG 0.570365 4_4:+61304227 Msa0692660:three_prime_UTR
ACAGGTTAGGTATCTGGAGA+TGG 0.571644 4_4:+61303396 Msa0692660:intron
AGATGTTGATGAGCTGGCAC+CGG 0.571765 4_4:-61301930 None:intergenic
ATTTAGGACCGTTACTATGG+AGG 0.572157 4_4:+61302895 Msa0692660:intron
CATGCACTTGAGATTTCTGA+TGG 0.575841 4_4:+61303475 Msa0692660:CDS
GCTGGAGCTGCAACTTAGAG+AGG 0.583221 4_4:+61303205 Msa0692660:CDS
AAGGGAGCTGGAGGCAACAA+AGG 0.588532 4_4:+61303658 Msa0692660:CDS
AAGAGAACTCAGACTCAGCT+GGG 0.590099 4_4:-61301815 None:intergenic
AGAGTAGTCTCCTCCAAGAA+GGG 0.590436 4_4:+61303580 Msa0692660:CDS
AATATGAATGTTGAAGGAGA+GGG 0.595497 4_4:-61302001 None:intergenic
GGATGGAACTGAACTTGTCA+AGG 0.596328 4_4:+61303000 Msa0692660:CDS
GTCACTCCTAAGGGAGCTAG+TGG 0.597101 4_4:+61303613 Msa0692660:CDS
TGACTCAGCTGTTGACCCCA+TGG 0.598789 4_4:+61304108 Msa0692660:three_prime_UTR
AAATAAACCTGAAGATGAGG+AGG 0.600134 4_4:+61303139 Msa0692660:intron
GAATATGAATGTTGAAGGAG+AGG 0.600142 4_4:-61302002 None:intergenic
CAGGACACTGCAGACAATGC+AGG 0.601280 4_4:+61303058 Msa0692660:CDS
TCGTGAATTCAATTGTAGCT+GGG 0.602234 4_4:+61303831 Msa0692660:three_prime_UTR
TGGGAAAACCTATTATCAGT+GGG 0.604807 4_4:+61302942 Msa0692660:CDS
AGAACTTCCCTCCATAGTAA+CGG 0.605346 4_4:-61302903 None:intergenic
CACGAGCAGCAATTCTCAAG+TGG 0.605488 4_4:-61303813 None:intergenic
CTGGGAAAACCTATTATCAG+TGG 0.606836 4_4:+61302941 Msa0692660:CDS
AAGGAAAAGTAGCAGCAGGA+AGG 0.607126 4_4:+61304324 Msa0692660:three_prime_UTR
CACTAGAACAACCGTAAAAG+GGG 0.608876 4_4:-61304613 None:intergenic
ACAATTGCATTGACTGCACA+AGG 0.614395 4_4:-61304509 None:intergenic
GTGGAGTTGTCACTTCTAAG+GGG 0.614783 4_4:+61303632 Msa0692660:CDS
TCGAAATGCTGATTTGATCA+GGG 0.621010 4_4:+61303897 Msa0692660:three_prime_UTR
TCAAAATGAATAATACTACA+AGG 0.628367 4_4:+61301887 Msa0692660:five_prime_UTR
GGAACTGAACTTGTCAAGGA+GGG 0.640908 4_4:+61303004 Msa0692660:CDS
GTGACTCAAGCAGTAAGACT+CGG 0.647491 4_4:+61303521 Msa0692660:CDS
TGGATGGTAACAAACTGATG+GGG 0.665765 4_4:+61304229 Msa0692660:three_prime_UTR
TGGAACTGAACTTGTCAAGG+AGG 0.668234 4_4:+61303003 Msa0692660:CDS
TGGAGTTGTCACTTCTAAGG+GGG 0.669080 4_4:+61303633 Msa0692660:CDS
GGATGGTAACAAACTGATGG+GGG 0.675199 4_4:+61304230 Msa0692660:three_prime_UTR
TTTGGTAAAACTGATGTGTG+TGG 0.689711 4_4:+61304432 Msa0692660:three_prime_UTR
AGGATAAATTCAAATCCTGG+AGG 0.721340 4_4:+61303439 Msa0692660:CDS
AATGTTACCGAAAAGATGCG+CGG 0.734112 4_4:-61302438 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATTAAAACAAATAAT+TGG - chr4_4:61302604-61302623 None:intergenic 10.0%
!!! TTTTTTGTTAAAATTTGATA+TGG + chr4_4:61304165-61304184 Msa0692660:intron 10.0%
!! TTTCATCAAATAAATGAAAT+TGG - chr4_4:61301845-61301864 None:intergenic 15.0%
!!! TTTATAATTTTATACAGGTT+AGG + chr4_4:61303383-61303402 Msa0692660:intron 15.0%
!! AAGCAAAATAAATTAAATGA+AGG - chr4_4:61303955-61303974 None:intergenic 15.0%
!!! TTTTTTCTATAGATTAAACA+TGG + chr4_4:61303989-61304008 Msa0692660:intron 15.0%
!! TCAAAATGAATAATACTACA+AGG + chr4_4:61301887-61301906 Msa0692660:five_prime_UTR 20.0%
!! TATATATGTAAGTAACTACT+TGG - chr4_4:61301969-61301988 None:intergenic 20.0%
!! AGGATTCATGAAAATAAAAA+CGG - chr4_4:61302262-61302281 None:intergenic 20.0%
!!! GTTTTTTAGTCTCTTAAATT+AGG + chr4_4:61302725-61302744 Msa0692660:intron 20.0%
!! CAACATTATCTATTCAATTA+AGG - chr4_4:61303341-61303360 None:intergenic 20.0%
!! TTGAATCTTCTTAATACAAT+AGG + chr4_4:61303926-61303945 Msa0692660:intron 20.0%
!! AAATTATGAAAACTCTCATA+GGG - chr4_4:61304144-61304163 None:intergenic 20.0%
!! AAAATTATGAAAACTCTCAT+AGG - chr4_4:61304145-61304164 None:intergenic 20.0%
! TACAAAACAGAGAAACAAAA+AGG - chr4_4:61301788-61301807 None:intergenic 25.0%
! GATGATTAATGTTATCGTAA+AGG - chr4_4:61302181-61302200 None:intergenic 25.0%
!! TTAGGCATTTTTCATCATTA+CGG + chr4_4:61302316-61302335 Msa0692660:intron 25.0%
! TCGTTGTTAATCAATTAAAG+TGG - chr4_4:61302690-61302709 None:intergenic 25.0%
! TATCAACAATCTTGAACAAA+AGG - chr4_4:61302781-61302800 None:intergenic 25.0%
! TGGATTATACTCAAAGATTA+TGG - chr4_4:61302862-61302881 None:intergenic 25.0%
! CCTAAATTACATCAAATACA+TGG - chr4_4:61302882-61302901 None:intergenic 25.0%
! CCATGTATTTGATGTAATTT+AGG + chr4_4:61302879-61302898 Msa0692660:intron 25.0%
! AAAACTCAAAACTACTGTTA+TGG + chr4_4:61302979-61302998 Msa0692660:CDS 25.0%
!!! TTATTTCTGAAGTTAATAGC+AGG - chr4_4:61303125-61303144 None:intergenic 25.0%
!!! CTATTGAATTGAATTTTGAC+TGG + chr4_4:61303231-61303250 Msa0692660:CDS 25.0%
!!! ATAGGGTATTTTTTTACCAT+GGG - chr4_4:61304127-61304146 None:intergenic 25.0%
!! TGAAATTTTACAAGAGAAGA+AGG + chr4_4:61304281-61304300 Msa0692660:three_prime_UTR 25.0%
AGAAACAAAAAGGCAAAGAA+AGG - chr4_4:61301778-61301797 None:intergenic 30.0%
AATATGAATGTTGAAGGAGA+GGG - chr4_4:61302004-61302023 None:intergenic 30.0%
TCTACGAATATGAATGTTGA+AGG - chr4_4:61302010-61302029 None:intergenic 30.0%
AAGGATTCATGAAAACGAAA+AGG - chr4_4:61302122-61302141 None:intergenic 30.0%
!!! CAGTAGTTTTGAGTTTTCTT+TGG - chr4_4:61302976-61302995 None:intergenic 30.0%
!!! TTGAATTGAATTTTGACTGG+AGG + chr4_4:61303234-61303253 Msa0692660:CDS 30.0%
! TATTTTACCTTAAGCATCTC+AGG - chr4_4:61303308-61303327 None:intergenic 30.0%
! TTTTATACAGGTTAGGTATC+TGG + chr4_4:61303390-61303409 Msa0692660:intron 30.0%
CTCACTTGTTCATCTAAATA+TGG + chr4_4:61303695-61303714 Msa0692660:exon 30.0%
TAGTTAAAAATCCCAAGGAT+TGG + chr4_4:61303770-61303789 Msa0692660:three_prime_UTR 30.0%
! AATAAGCCAAACTTGTTTGT+TGG + chr4_4:61304058-61304077 Msa0692660:three_prime_UTR 30.0%
GTCATCAGAATTATTCATAG+TGG - chr4_4:61304092-61304111 None:intergenic 30.0%
!!! TAGGGTATTTTTTTACCATG+GGG - chr4_4:61304126-61304145 None:intergenic 30.0%
!!! CATAGGGTATTTTTTTACCA+TGG - chr4_4:61304128-61304147 None:intergenic 30.0%
!!! TTTTTTGTTGCAACAGCTAA+AGG + chr4_4:61304200-61304219 Msa0692660:intron 30.0%
TCAACTTTACTGTTTCAATC+TGG + chr4_4:61304376-61304395 Msa0692660:three_prime_UTR 30.0%
! CAAACTCTTCGTGATAATTT+TGG + chr4_4:61304414-61304433 Msa0692660:three_prime_UTR 30.0%
TACAGTACAAATCAGTTACT+TGG - chr4_4:61304551-61304570 None:intergenic 30.0%
GAATATGAATGTTGAAGGAG+AGG - chr4_4:61302005-61302024 None:intergenic 35.0%
! TCTCAACGTTTTCATCTTTC+AGG + chr4_4:61302074-61302093 Msa0692660:five_prime_UTR 35.0%
CATCAACAACAACAACAACA+AGG - chr4_4:61302141-61302160 None:intergenic 35.0%
GATGCTGATGAATCTTTCTT+CGG + chr4_4:61302225-61302244 Msa0692660:intron 35.0%
TTCACTGATGAATCTTCGTT+CGG + chr4_4:61302284-61302303 Msa0692660:intron 35.0%
! ATGAATCTTCGTTCGGTTTT+AGG + chr4_4:61302291-61302310 Msa0692660:intron 35.0%
!!! TTCGTTCGGTTTTAGGTTTT+AGG + chr4_4:61302298-61302317 Msa0692660:intron 35.0%
ATGAATCTTCGTTCGATTTC+AGG + chr4_4:61302395-61302414 Msa0692660:intron 35.0%
! GTAATTTAGGACCGTTACTA+TGG + chr4_4:61302892-61302911 Msa0692660:intron 35.0%
TGGGAAAACCTATTATCAGT+GGG + chr4_4:61302942-61302961 Msa0692660:CDS 35.0%
CTCAAAACTACTGTTATGGA+TGG + chr4_4:61302983-61303002 Msa0692660:CDS 35.0%
CAGAAATAAACCTGAAGATG+AGG + chr4_4:61303136-61303155 Msa0692660:intron 35.0%
AAATAAACCTGAAGATGAGG+AGG + chr4_4:61303139-61303158 Msa0692660:intron 35.0%
ATGAAAGAGATAAGGAAGTC+AGG + chr4_4:61303419-61303438 Msa0692660:CDS 35.0%
GTCAGGATAAATTCAAATCC+TGG + chr4_4:61303436-61303455 Msa0692660:CDS 35.0%
AGGATAAATTCAAATCCTGG+AGG + chr4_4:61303439-61303458 Msa0692660:CDS 35.0%
AAGCGTAGTTAAAAATCCCA+AGG + chr4_4:61303765-61303784 Msa0692660:three_prime_UTR 35.0%
TTAAAAATCCCAAGGATTGG+TGG + chr4_4:61303773-61303792 Msa0692660:three_prime_UTR 35.0%
TCGTGAATTCAATTGTAGCT+GGG + chr4_4:61303831-61303850 Msa0692660:three_prime_UTR 35.0%
!! ATCGAAATGCTGATTTGATC+AGG + chr4_4:61303896-61303915 Msa0692660:three_prime_UTR 35.0%
!! TCGAAATGCTGATTTGATCA+GGG + chr4_4:61303897-61303916 Msa0692660:three_prime_UTR 35.0%
AACATGGACAATTGATGCAA+AGG + chr4_4:61304005-61304024 Msa0692660:three_prime_UTR 35.0%
ACATGGACAATTGATGCAAA+GGG + chr4_4:61304006-61304025 Msa0692660:three_prime_UTR 35.0%
CTTTGACCAACAAACAAGTT+TGG - chr4_4:61304067-61304086 None:intergenic 35.0%
TTTACAAGAGAAGAAGGAGA+AGG + chr4_4:61304287-61304306 Msa0692660:three_prime_UTR 35.0%
TTTGGTAAAACTGATGTGTG+TGG + chr4_4:61304432-61304451 Msa0692660:three_prime_UTR 35.0%
TCACTAGAACAACCGTAAAA+GGG - chr4_4:61304617-61304636 None:intergenic 35.0%
TTCACTAGAACAACCGTAAA+AGG - chr4_4:61304618-61304637 None:intergenic 35.0%
! TGTTACAGATGTTGATGAGC+TGG - chr4_4:61301939-61301958 None:intergenic 40.0%
CGAAGATTCATCAGTGAACA+AGG - chr4_4:61302282-61302301 None:intergenic 40.0%
CGAAGATTCATCAGTGAACA+AGG - chr4_4:61302282-61302301 None:intergenic 40.0%
AATGTTACCGAAAAGATGCG+CGG - chr4_4:61302441-61302460 None:intergenic 40.0%
! ATTTAGGACCGTTACTATGG+AGG + chr4_4:61302895-61302914 Msa0692660:intron 40.0%
! TTTAGGACCGTTACTATGGA+GGG + chr4_4:61302896-61302915 Msa0692660:intron 40.0%
AGAACTTCCCTCCATAGTAA+CGG - chr4_4:61302906-61302925 None:intergenic 40.0%
! ACTATGGAGGGAAGTTCTAA+AGG + chr4_4:61302908-61302927 Msa0692660:exon 40.0%
! TTCTAAAGGTGATCATGTCC+TGG + chr4_4:61302922-61302941 Msa0692660:CDS 40.0%
TCTAAAGGTGATCATGTCCT+GGG + chr4_4:61302923-61302942 Msa0692660:CDS 40.0%
! CACTGATAATAGGTTTTCCC+AGG - chr4_4:61302943-61302962 None:intergenic 40.0%
CTGGGAAAACCTATTATCAG+TGG + chr4_4:61302941-61302960 Msa0692660:CDS 40.0%
CTACGTTTCCCACTGATAAT+AGG - chr4_4:61302953-61302972 None:intergenic 40.0%
AATGACACCTGAGATGCTTA+AGG + chr4_4:61303298-61303317 Msa0692660:CDS 40.0%
GGAGATGGATGAAAGAGATA+AGG + chr4_4:61303411-61303430 Msa0692660:CDS 40.0%
! TCAAGTGCATGCTCTTTTCT+AGG - chr4_4:61303466-61303485 None:intergenic 40.0%
CATGCACTTGAGATTTCTGA+TGG + chr4_4:61303475-61303494 Msa0692660:CDS 40.0%
! TAGTGGAGTTGTCACTTCTA+AGG + chr4_4:61303630-61303649 Msa0692660:CDS 40.0%
! AGTGGAGTTGTCACTTCTAA+GGG + chr4_4:61303631-61303650 Msa0692660:CDS 40.0%
CTCGTGAATTCAATTGTAGC+TGG + chr4_4:61303830-61303849 Msa0692660:three_prime_UTR 40.0%
CTGGATGGTAACAAACTGAT+GGG + chr4_4:61304228-61304247 Msa0692660:three_prime_UTR 40.0%
TGGATGGTAACAAACTGATG+GGG + chr4_4:61304229-61304248 Msa0692660:three_prime_UTR 40.0%
GAAGGAGAAGGAGAAAGAAA+AGG + chr4_4:61304305-61304324 Msa0692660:three_prime_UTR 40.0%
AGAAAAGGAAAAGTAGCAGC+AGG + chr4_4:61304320-61304339 Msa0692660:three_prime_UTR 40.0%
!! ACAATTGCATTGACTGCACA+AGG - chr4_4:61304512-61304531 None:intergenic 40.0%
CCGTAAAAGGGGTCAAATTT+GGG - chr4_4:61304605-61304624 None:intergenic 40.0%
! CCCAAATTTGACCCCTTTTA+CGG + chr4_4:61304602-61304621 Msa0692660:three_prime_UTR 40.0%
ACCGTAAAAGGGGTCAAATT+TGG - chr4_4:61304606-61304625 None:intergenic 40.0%
CACTAGAACAACCGTAAAAG+GGG - chr4_4:61304616-61304635 None:intergenic 40.0%
AAGAGAACTCAGACTCAGCT+GGG - chr4_4:61301818-61301837 None:intergenic 45.0%
AAAGAGAACTCAGACTCAGC+TGG - chr4_4:61301819-61301838 None:intergenic 45.0%
CAATTCCGATTGAAGCTTGC+CGG + chr4_4:61301911-61301930 Msa0692660:five_prime_UTR 45.0%
CTTTCTCTCATCGCTCTCAA+CGG + chr4_4:61302042-61302061 Msa0692660:five_prime_UTR 45.0%
! CATCTTTCCGCGCATCTTTT+CGG + chr4_4:61302431-61302450 Msa0692660:five_prime_UTR 45.0%
GGATGGAACTGAACTTGTCA+AGG + chr4_4:61303000-61303019 Msa0692660:CDS 45.0%
TGGAACTGAACTTGTCAAGG+AGG + chr4_4:61303003-61303022 Msa0692660:CDS 45.0%
GGAACTGAACTTGTCAAGGA+GGG + chr4_4:61303004-61303023 Msa0692660:CDS 45.0%
TGATCCTTTCACTGAAGAGC+TGG + chr4_4:61303187-61303206 Msa0692660:CDS 45.0%
ACAGGTTAGGTATCTGGAGA+TGG + chr4_4:61303396-61303415 Msa0692660:intron 45.0%
GTGACTCAAGCAGTAAGACT+CGG + chr4_4:61303521-61303540 Msa0692660:CDS 45.0%
AAGAGTAGTCTCCTCCAAGA+AGG + chr4_4:61303579-61303598 Msa0692660:CDS 45.0%
AGAGTAGTCTCCTCCAAGAA+GGG + chr4_4:61303580-61303599 Msa0692660:CDS 45.0%
AAGTGGAGTTGTCACTCCTA+AGG + chr4_4:61303603-61303622 Msa0692660:CDS 45.0%
AGTGGAGTTGTCACTCCTAA+GGG + chr4_4:61303604-61303623 Msa0692660:CDS 45.0%
! GTGGAGTTGTCACTTCTAAG+GGG + chr4_4:61303632-61303651 Msa0692660:CDS 45.0%
! TGGAGTTGTCACTTCTAAGG+GGG + chr4_4:61303633-61303652 Msa0692660:CDS 45.0%
GAATTCAATTGTAGCTGGGC+AGG + chr4_4:61303835-61303854 Msa0692660:three_prime_UTR 45.0%
GCTGGATGGTAACAAACTGA+TGG + chr4_4:61304227-61304246 Msa0692660:three_prime_UTR 45.0%
GGATGGTAACAAACTGATGG+GGG + chr4_4:61304230-61304249 Msa0692660:three_prime_UTR 45.0%
AGAGAAGAAGGAGAAGGAGA+AGG + chr4_4:61304293-61304312 Msa0692660:three_prime_UTR 45.0%
AAGGAAAAGTAGCAGCAGGA+AGG + chr4_4:61304324-61304343 Msa0692660:three_prime_UTR 45.0%
!!! AATTATTTATAATTTTATAC+AGG + chr4_4:61303378-61303397 Msa0692660:intron 5.0%
CGGCAAGCTTCAATCGGAAT+TGG - chr4_4:61301913-61301932 None:intergenic 50.0%
! AGATGTTGATGAGCTGGCAC+CGG - chr4_4:61301933-61301952 None:intergenic 50.0%
GAACTTGTCAAGGAGGGAAG+TGG + chr4_4:61303010-61303029 Msa0692660:CDS 50.0%
TCAAGGAGGGAAGTGGTAGT+AGG + chr4_4:61303017-61303036 Msa0692660:CDS 50.0%
AGGGAAGTGGTAGTAGGCAA+GGG + chr4_4:61303023-61303042 Msa0692660:CDS 50.0%
GCAAGGGAAGTCACATATCC+AGG + chr4_4:61303039-61303058 Msa0692660:CDS 50.0%
AGCTCCAGCTCTTCAGTGAA+AGG - chr4_4:61303194-61303213 None:intergenic 50.0%
!!! TGCTCTTTTCTAGGACCTCC+AGG - chr4_4:61303457-61303476 None:intergenic 50.0%
AACTCCACTTCCCTTCTTGG+AGG - chr4_4:61303593-61303612 None:intergenic 50.0%
GACAACTCCACTTCCCTTCT+TGG - chr4_4:61303596-61303615 None:intergenic 50.0%
ACAACTCCACTAGCTCCCTT+AGG - chr4_4:61303622-61303641 None:intergenic 50.0%
! TGTCACTTCTAAGGGGGCTA+AGG + chr4_4:61303639-61303658 Msa0692660:CDS 50.0%
! GTCACTTCTAAGGGGGCTAA+GGG + chr4_4:61303640-61303659 Msa0692660:CDS 50.0%
!! TCCCAAGGATTGGTGGACTA+AGG + chr4_4:61303780-61303799 Msa0692660:three_prime_UTR 50.0%
GCCTTAGTCCACCAATCCTT+GGG - chr4_4:61303784-61303803 None:intergenic 50.0%
AGCCTTAGTCCACCAATCCT+TGG - chr4_4:61303785-61303804 None:intergenic 50.0%
CACGAGCAGCAATTCTCAAG+TGG - chr4_4:61303816-61303835 None:intergenic 50.0%
ATGCAAAGGGACGCCAAGTT+GGG + chr4_4:61304019-61304038 Msa0692660:three_prime_UTR 50.0%
GCAACAGCTAAAGGCTTTGC+TGG + chr4_4:61304209-61304228 Msa0692660:intron 50.0%
CAGCTAAAGGCTTTGCTGGA+TGG + chr4_4:61304213-61304232 Msa0692660:intron 50.0%
!! TGGCACCGGCAAGCTTCAAT+CGG - chr4_4:61301919-61301938 None:intergenic 55.0%
GAGGGAAGTGGTAGTAGGCA+AGG + chr4_4:61303022-61303041 Msa0692660:CDS 55.0%
CTGCATTGTCTGCAGTGTCC+TGG - chr4_4:61303060-61303079 None:intergenic 55.0%
CAGGACACTGCAGACAATGC+AGG + chr4_4:61303058-61303077 Msa0692660:CDS 55.0%
CACAGCTCCTCCTCATCTTC+AGG - chr4_4:61303149-61303168 None:intergenic 55.0%
GCTGGAGCTGCAACTTAGAG+AGG + chr4_4:61303205-61303224 Msa0692660:CDS 55.0%
GTCTCCTCCAAGAAGGGAAG+TGG + chr4_4:61303586-61303605 Msa0692660:CDS 55.0%
GTCACTCCTAAGGGAGCTAG+TGG + chr4_4:61303613-61303632 Msa0692660:CDS 55.0%
AAGGGAGCTGGAGGCAACAA+AGG + chr4_4:61303658-61303677 Msa0692660:CDS 55.0%
GATGCAAAGGGACGCCAAGT+TGG + chr4_4:61304018-61304037 Msa0692660:three_prime_UTR 55.0%
! AAGGGACGCCAAGTTGGGAA+AGG + chr4_4:61304024-61304043 Msa0692660:three_prime_UTR 55.0%
! AGGGACGCCAAGTTGGGAAA+GGG + chr4_4:61304025-61304044 Msa0692660:three_prime_UTR 55.0%
GTCAGACCCCTTTCCCAACT+TGG - chr4_4:61304035-61304054 None:intergenic 55.0%
TGACTCAGCTGTTGACCCCA+TGG + chr4_4:61304108-61304127 Msa0692660:three_prime_UTR 55.0%
TCTAAGGGGGCTAAGGGAGC+TGG + chr4_4:61303646-61303665 Msa0692660:CDS 60.0%
! GGGACGCCAAGTTGGGAAAG+GGG + chr4_4:61304026-61304045 Msa0692660:three_prime_UTR 60.0%
AAGGGGGCTAAGGGAGCTGG+AGG + chr4_4:61303649-61303668 Msa0692660:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 61301751 61304657 61301751 ID=Msa0692660;Name=Msa0692660
chr4_4 mRNA 61301751 61304657 61301751 ID=Msa0692660-mRNA-1;Parent=Msa0692660;Name=Msa0692660-mRNA-1;_AED=0.07;_eAED=0.07;_QI=391|1|1|1|0.66|0.71|7|778|215
chr4_4 exon 61301751 61302095 61301751 ID=Msa0692660-mRNA-1:exon:16901;Parent=Msa0692660-mRNA-1
chr4_4 exon 61302417 61302452 61302417 ID=Msa0692660-mRNA-1:exon:16902;Parent=Msa0692660-mRNA-1
chr4_4 exon 61302901 61303079 61302901 ID=Msa0692660-mRNA-1:exon:16903;Parent=Msa0692660-mRNA-1
chr4_4 exon 61303153 61303319 61303153 ID=Msa0692660-mRNA-1:exon:16904;Parent=Msa0692660-mRNA-1
chr4_4 exon 61303400 61303918 61303400 ID=Msa0692660-mRNA-1:exon:16905;Parent=Msa0692660-mRNA-1
chr4_4 exon 61304001 61304129 61304001 ID=Msa0692660-mRNA-1:exon:16906;Parent=Msa0692660-mRNA-1
chr4_4 exon 61304216 61304657 61304216 ID=Msa0692660-mRNA-1:exon:16907;Parent=Msa0692660-mRNA-1
chr4_4 five_prime_UTR 61301751 61302095 61301751 ID=Msa0692660-mRNA-1:five_prime_utr;Parent=Msa0692660-mRNA-1
chr4_4 five_prime_UTR 61302417 61302452 61302417 ID=Msa0692660-mRNA-1:five_prime_utr;Parent=Msa0692660-mRNA-1
chr4_4 five_prime_UTR 61302901 61302910 61302901 ID=Msa0692660-mRNA-1:five_prime_utr;Parent=Msa0692660-mRNA-1
chr4_4 CDS 61302911 61303079 61302911 ID=Msa0692660-mRNA-1:cds;Parent=Msa0692660-mRNA-1
chr4_4 CDS 61303153 61303319 61303153 ID=Msa0692660-mRNA-1:cds;Parent=Msa0692660-mRNA-1
chr4_4 CDS 61303400 61303711 61303400 ID=Msa0692660-mRNA-1:cds;Parent=Msa0692660-mRNA-1
chr4_4 three_prime_UTR 61303712 61303918 61303712 ID=Msa0692660-mRNA-1:three_prime_utr;Parent=Msa0692660-mRNA-1
chr4_4 three_prime_UTR 61304001 61304129 61304001 ID=Msa0692660-mRNA-1:three_prime_utr;Parent=Msa0692660-mRNA-1
chr4_4 three_prime_UTR 61304216 61304657 61304216 ID=Msa0692660-mRNA-1:three_prime_utr;Parent=Msa0692660-mRNA-1
Gene Sequence

>Msa0692660

ATGGAGGGAAGTTCTAAAGGTGATCATGTCCTGGGAAAACCTATTATCAGTGGGAAACGTAGCCAAAGAAAACTCAAAACTACTGTTATGGATGGAACTGAACTTGTCAAGGAGGGAAGTGGTAGTAGGCAAGGGAAGTCACATATCCAGGACACTGCAGACAATGCAGATGAGGAGGAGCTGTGCGATTCATTGCTTTTTGTTGATCCTTTCACTGAAGAGCTGGAGCTGCAACTTAGAGAGGCTGCTATTGAATTGAATTTTGACTGGAGGAAGATCAAGAGATCTAAAAAATATGCTAATATTTTTAAAGAAATGACACCTGAGATGCTTAAGGTTAGGTATCTGGAGATGGATGAAAGAGATAAGGAAGTCAGGATAAATTCAAATCCTGGAGGTCCTAGAAAAGAGCATGCACTTGAGATTTCTGATGGTAGCTCAAGCAGAAAGACTGATAGTGACTCAAGCAGTAAGACTCGGACAGATGATGATTCTATTCAGCTGAGAAGAAGTGCAAGAGTAGTCTCCTCCAAGAAGGGAAGTGGAGTTGTCACTCCTAAGGGAGCTAGTGGAGTTGTCACTTCTAAGGGGGCTAAGGGAGCTGGAGGCAACAAAGGTTCTCTTACATTCACTCACTTGTTCATCTAA
Protein sequence

>Msa0692660

MEGSSKGDHVLGKPIISGKRSQRKLKTTVMDGTELVKEGSGSRQGKSHIQDTADNADEEELCDSLLFVDPFTEELELQLREAAIELNFDWRKIKRSKKYANIFKEMTPEMLKVRYLEMDERDKEVRINSNPGGPRKEHALEISDGSSSRKTDSDSSSKTRTDDDSIQLRRSARVVSSKKGSGVVTPKGASGVVTSKGAKGAGGNKGSLTFTHLFI*