AlfalfaGEDB | Msa0705780

M. sativa cultivar zhongmu-4 / Msa0705780

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009835 GO:0009889 GO:0009908 GO:0009987 GO:0010150 GO:0010154 GO:0010468 GO:0010556 GO:0016049 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0090567 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705780	XP_003630281.1	87.778	90	4	3	1	83	96	185	2.96e-43	148

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705780	sp\|Q8GWK6\|NC104_ARATH	41.176	102	30	5	1	82	96	187	3.73e-13	64.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705780	G7LE35	87.778	90	4	3	1	83	96	185	1.42e-43	148

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0705780	Msa0721280	0.821684	3.542711e-53	-8.615850e-47
Msa0705780	Msa1357890	0.823663	1.226973e-53	-8.615850e-47
Msa0705780	Msa1398090	0.825717	4.026474e-54	-8.615850e-47
Msa0376910	Msa0705780	0.802189	6.311785e-49	-8.615850e-47
Msa0484780	Msa0705780	0.800716	1.264807e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705780	MtrunA17_Chr8g0384351	87.778	90	4	3	1	83	96	185	2.73e-47	148
Msa0705780	MtrunA17_Chr3g0130321	44.860	107	35	5	1	83	102	208	2.13e-19	77.8
Msa0705780	MtrunA17_Chr4g0050701	43.750	96	33	4	1	83	104	191	1.15e-14	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705780	AT5G64530.1	41.176	102	30	5	1	82	96	187	3.79e-14	64.3

Find 33 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAAAACCTATAAATAAAT+TGG	0.146052	4_4:+78598333	Msa0705780:five_prime_UTR
TTCATTCCCAATTTATTTAT+AGG	0.164515	4_4:-78598340	None:intergenic
AATGGATATTGGAAGGAAAT+TGG	0.199332	4_4:+78598307	Msa0705780:five_prime_UTR
AAGCTAATTATTAACTAATT+AGG	0.204594	4_4:-78598761	None:intergenic
GAAAATAGAAGCACTGAAAA+TGG	0.301344	4_4:+78598289	Msa0705780:five_prime_UTR
ATGGATATTGGAAGGAAATT+GGG	0.330470	4_4:+78598308	Msa0705780:five_prime_UTR
GTGTGAGACAGGATCATAAT+TGG	0.363529	4_4:+78598591	Msa0705780:intron
GAAGCACTGAAAATGGATAT+TGG	0.365813	4_4:+78598296	Msa0705780:five_prime_UTR
TTGATCAAGATCATCATCTA+AGG	0.370976	4_4:-78598729	None:intergenic
AGTACCTTGTATTCAACCTT+GGG	0.405124	4_4:+78598365	Msa0705780:CDS
ATATACATGCCTTTGTAAAC+AGG	0.412177	4_4:+78598217	None:intergenic
GATGAGGATATTGATGATAA+TGG	0.434866	4_4:+78598671	Msa0705780:CDS
CTTCCCCAAGGTTGAATACA+AGG	0.441845	4_4:-78598369	None:intergenic
ACAAAACCTATAAATAAATT+GGG	0.452946	4_4:+78598334	Msa0705780:five_prime_UTR
AATTGAAACAAGTTGGGTAA+TGG	0.457936	4_4:+78598393	Msa0705780:CDS
GGGAAGGAATTGAAACAAGT+TGG	0.476317	4_4:+78598386	Msa0705780:CDS
GGAAGGAATTGAAACAAGTT+GGG	0.495348	4_4:+78598387	Msa0705780:CDS
ATATCATATTTCCTCATCTC+CGG	0.517814	4_4:+78598420	Msa0705780:CDS
AGGATCATAATTGGAGCAGA+TGG	0.520438	4_4:+78598600	Msa0705780:intron
TAATTATTAACTAATTAGGT+AGG	0.520877	4_4:-78598757	None:intergenic
TGGATATTGGAAGGAAATTG+GGG	0.532640	4_4:+78598309	Msa0705780:five_prime_UTR
AAGTACCTTGTATTCAACCT+TGG	0.541631	4_4:+78598364	Msa0705780:CDS
CTTGTATTCAACCTTGGGGA+AGG	0.542660	4_4:+78598370	Msa0705780:CDS
TTGTTTCAATTCCTTCCCCA+AGG	0.571160	4_4:-78598381	None:intergenic
GGATCATAATTGGAGCAGAT+GGG	0.571776	4_4:+78598601	Msa0705780:intron
CACTGAAAATGGATATTGGA+AGG	0.575605	4_4:+78598300	Msa0705780:five_prime_UTR
GAAAAGAAGATGTCACATCA+AGG	0.579347	4_4:+78598641	Msa0705780:CDS
ACAATCACTTACCGGAGATG+AGG	0.598757	4_4:-78598431	None:intergenic
AATGCTTTACCTGTTTACAA+AGG	0.615310	4_4:-78598226	None:intergenic
ACAGGTAAAGCATTGTCAAG+TGG	0.622513	4_4:+78598235	Msa0705780:five_prime_UTR
ACATCAAGGTGCAAGCGATG+AGG	0.654589	4_4:+78598655	Msa0705780:CDS
GTACCTTGTATTCAACCTTG+GGG	0.661844	4_4:+78598366	Msa0705780:CDS
ATAATGGAACAGATGTGTCA+TGG	0.669182	4_4:+78598687	Msa0705780:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAAACCTATAAATAAATT+GGG	+	chr4_4:78598334-78598353	Msa0705780:five_prime_UTR	15.0%
!!!	TAATTTTTTTCCACATTAAT+TGG	-	chr4_4:78598522-78598541	None:intergenic	15.0%
!!	TAATTATTAACTAATTAGGT+AGG	-	chr4_4:78598760-78598779	None:intergenic	15.0%
!!	AAGCTAATTATTAACTAATT+AGG	-	chr4_4:78598764-78598783	None:intergenic	15.0%
!!	CACAAAACCTATAAATAAAT+TGG	+	chr4_4:78598333-78598352	Msa0705780:five_prime_UTR	20.0%
!!	TTCATTCCCAATTTATTTAT+AGG	-	chr4_4:78598343-78598362	None:intergenic	20.0%
!!!	ATTTGTTGTGATTTATGAAT+AGG	-	chr4_4:78598475-78598494	None:intergenic	20.0%
	AATGCTTTACCTGTTTACAA+AGG	-	chr4_4:78598229-78598248	None:intergenic	30.0%
!!	GAAAATAGAAGCACTGAAAA+TGG	+	chr4_4:78598289-78598308	Msa0705780:five_prime_UTR	30.0%
	AATGGATATTGGAAGGAAAT+TGG	+	chr4_4:78598307-78598326	Msa0705780:five_prime_UTR	30.0%
	ATGGATATTGGAAGGAAATT+GGG	+	chr4_4:78598308-78598327	Msa0705780:five_prime_UTR	30.0%
!	AATTGAAACAAGTTGGGTAA+TGG	+	chr4_4:78598393-78598412	Msa0705780:CDS	30.0%
	ATATCATATTTCCTCATCTC+CGG	+	chr4_4:78598420-78598439	Msa0705780:CDS	30.0%
	AACAAAACACAATCACTTAC+CGG	-	chr4_4:78598442-78598461	None:intergenic	30.0%
	CAAATCATGACCAATTAATG+TGG	+	chr4_4:78598509-78598528	Msa0705780:intron	30.0%
!!	TATTTTTGATTGTGTGAGAC+AGG	+	chr4_4:78598580-78598599	Msa0705780:intron	30.0%
!	GATGAGGATATTGATGATAA+TGG	+	chr4_4:78598671-78598690	Msa0705780:CDS	30.0%
	TTGATCAAGATCATCATCTA+AGG	-	chr4_4:78598732-78598751	None:intergenic	30.0%
!	GCTTCTATTTTCCTTCACTT+CGG	-	chr4_4:78598281-78598300	None:intergenic	35.0%
!!	GAAGCACTGAAAATGGATAT+TGG	+	chr4_4:78598296-78598315	Msa0705780:five_prime_UTR	35.0%
	CACTGAAAATGGATATTGGA+AGG	+	chr4_4:78598300-78598319	Msa0705780:five_prime_UTR	35.0%
	TGGATATTGGAAGGAAATTG+GGG	+	chr4_4:78598309-78598328	Msa0705780:five_prime_UTR	35.0%
	AAGTACCTTGTATTCAACCT+TGG	+	chr4_4:78598364-78598383	Msa0705780:CDS	35.0%
	AGTACCTTGTATTCAACCTT+GGG	+	chr4_4:78598365-78598384	Msa0705780:CDS	35.0%
	GGAAGGAATTGAAACAAGTT+GGG	+	chr4_4:78598387-78598406	Msa0705780:CDS	35.0%
	GAAAAGAAGATGTCACATCA+AGG	+	chr4_4:78598641-78598660	Msa0705780:CDS	35.0%
!	ATAATGGAACAGATGTGTCA+TGG	+	chr4_4:78598687-78598706	Msa0705780:CDS	35.0%
!!	ACAGGTAAAGCATTGTCAAG+TGG	+	chr4_4:78598235-78598254	Msa0705780:five_prime_UTR	40.0%
!!!	CTACTTTTTCACCGAAGTGA+AGG	+	chr4_4:78598267-78598286	Msa0705780:five_prime_UTR	40.0%
	GTACCTTGTATTCAACCTTG+GGG	+	chr4_4:78598366-78598385	Msa0705780:CDS	40.0%
	TTGTTTCAATTCCTTCCCCA+AGG	-	chr4_4:78598384-78598403	None:intergenic	40.0%
	GGGAAGGAATTGAAACAAGT+TGG	+	chr4_4:78598386-78598405	Msa0705780:CDS	40.0%
	GTGTGAGACAGGATCATAAT+TGG	+	chr4_4:78598591-78598610	Msa0705780:intron	40.0%
	AGGATCATAATTGGAGCAGA+TGG	+	chr4_4:78598600-78598619	Msa0705780:intron	40.0%
	GGATCATAATTGGAGCAGAT+GGG	+	chr4_4:78598601-78598620	Msa0705780:intron	40.0%
	CTTCCCCAAGGTTGAATACA+AGG	-	chr4_4:78598372-78598391	None:intergenic	45.0%
	CTTGTATTCAACCTTGGGGA+AGG	+	chr4_4:78598370-78598389	Msa0705780:CDS	45.0%
	ACAATCACTTACCGGAGATG+AGG	-	chr4_4:78598434-78598453	None:intergenic	45.0%
	ACATCAAGGTGCAAGCGATG+AGG	+	chr4_4:78598655-78598674	Msa0705780:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	78598222	78598787	78598222	ID=Msa0705780;Name=Msa0705780
chr4_4	mRNA	78598222	78598787	78598222	ID=Msa0705780-mRNA-1;Parent=Msa0705780;Name=Msa0705780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=136\|1\|1\|1\|1\|1\|2\|18\|83
chr4_4	exon	78598222	78598441	78598222	ID=Msa0705780-mRNA-1:exon:25094;Parent=Msa0705780-mRNA-1
chr4_4	exon	78598602	78598787	78598602	ID=Msa0705780-mRNA-1:exon:25095;Parent=Msa0705780-mRNA-1
chr4_4	five_prime_UTR	78598222	78598357	78598222	ID=Msa0705780-mRNA-1:five_prime_utr;Parent=Msa0705780-mRNA-1
chr4_4	CDS	78598358	78598441	78598358	ID=Msa0705780-mRNA-1:cds;Parent=Msa0705780-mRNA-1
chr4_4	CDS	78598602	78598769	78598602	ID=Msa0705780-mRNA-1:cds;Parent=Msa0705780-mRNA-1
chr4_4	three_prime_UTR	78598770	78598787	78598770	ID=Msa0705780-mRNA-1:three_prime_utr;Parent=Msa0705780-mRNA-1

Gene Sequence	>Msa0705780 ATGAAGAAGTACCTTGTATTCAACCTTGGGGAAGGAATTGAAACAAGTTGGGTAATGGAAGAATATCATATTTCCTCATCTCCGGATCATAATTGGAGCAGATGGGTTTTATGCAAAGTTTATGAAAAGAAGATGTCACATCAAGGTGCAAGCGATGAGGATATTGATGATAATGGAACAGATGTGTCATGGCTAGATGAAGTGTTTATGTCCTTAGATGATGATCTTGATCAAACAAGCCTACCTAATTAG
Protein sequence	>Msa0705780 MKKYLVFNLGEGIETSWVMEEYHISSSPDHNWSRWVLCKVYEKKMSHQGASDEDIDDNGTDVSWLDEVFMSLDDDLDQTSLPN*