Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0715690 | XP_003611853.1 | 96.894 | 161 | 5 | 0 | 1 | 161 | 1 | 161 | 4.37e-103 | 302 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0715690 | G7K9G8 | 96.894 | 161 | 5 | 0 | 1 | 161 | 1 | 161 | 2.09e-103 | 302 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0715690 | Msa0715760 | 0.807605 | 4.659435e-50 | -8.615850e-47 |
| Msa0715690 | Msa0910400 | 0.810790 | 9.673109e-51 | -8.615850e-47 |
| Msa0715690 | Msa0975080 | 0.810094 | 1.367190e-50 | -8.615850e-47 |
| Msa0414070 | Msa0715690 | 0.808867 | 2.507247e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0715690 | MtrunA17_Chr5g0403071 | 96.894 | 161 | 5 | 0 | 1 | 161 | 1 | 161 | 4.02e-107 | 302 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0715690 | AT2G23670.1 | 65.546 | 119 | 41 | 0 | 43 | 161 | 49 | 167 | 3.50e-45 | 146 |
Find 50 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCGGTGGTGAATGGTATTT+TGG | 0.159428 | 5_1:-8772499 | Msa0715690:CDS |
| GGATTGGAGACGGCGGAAAA+AGG | 0.331002 | 5_1:-8772648 | Msa0715690:CDS |
| GGCACAGGCGATTGTGGTGG+CGG | 0.376810 | 5_1:-8772553 | Msa0715690:CDS |
| CGCAAGATATTCCTCTGTTC+GGG | 0.386647 | 5_1:-8772746 | Msa0715690:CDS |
| AGTCATGACTTTGAACCTTC+AGG | 0.395878 | 5_1:+8772474 | None:intergenic |
| GGCGAGGGAATCAGTGTCTA+TGG | 0.402052 | 5_1:-8772769 | Msa0715690:CDS |
| GCGCAAGATATTCCTCTGTT+CGG | 0.424152 | 5_1:-8772747 | Msa0715690:CDS |
| GTAACAGCGTCTCCATCATC+CGG | 0.439205 | 5_1:-8772837 | Msa0715690:CDS |
| GGGGCTTGGGAGTTTGGCAC+AGG | 0.442466 | 5_1:-8772568 | Msa0715690:CDS |
| CATGTTGAAACGAGAGAGTT+GGG | 0.445635 | 5_1:+8772913 | None:intergenic |
| CACGGCGTCTACTGCTTTAA+CGG | 0.459616 | 5_1:-8772790 | Msa0715690:CDS |
| GGATTTGAATCCGCGGAGAA+AGG | 0.475547 | 5_1:-8772669 | Msa0715690:CDS |
| TGAGAAGGCGGAACTAGGAT+TGG | 0.477543 | 5_1:-8772622 | Msa0715690:CDS |
| ATATTCCTCTGTTCGGGATA+CGG | 0.482009 | 5_1:-8772740 | Msa0715690:CDS |
| GTTGCTTCAGCGGTGGTGAA+TGG | 0.484024 | 5_1:-8772507 | Msa0715690:CDS |
| GCATGAAAACGACGTCGCAT+TGG | 0.485174 | 5_1:+8772881 | None:intergenic |
| AGGATTGGAGGAGGCGGAGA+AGG | 0.488587 | 5_1:-8772607 | Msa0715690:CDS |
| GCACAGGCGATTGTGGTGGC+GGG | 0.499057 | 5_1:-8772552 | Msa0715690:CDS |
| ACATGTTGAAACGAGAGAGT+TGG | 0.500814 | 5_1:+8772912 | None:intergenic |
| GGACTTGAGAAGGCGGAACT+AGG | 0.502229 | 5_1:-8772627 | Msa0715690:CDS |
| TGAATCCGCGGAGAAAGGAT+TGG | 0.503269 | 5_1:-8772664 | Msa0715690:CDS |
| AACTCCCTTCGCCCGGATGA+TGG | 0.506449 | 5_1:+8772825 | None:intergenic |
| GCTGAGGAGATTGTGAAGGA+AGG | 0.507532 | 5_1:-8772690 | Msa0715690:CDS |
| CGTCTCCATCATCCGGGCGA+AGG | 0.507772 | 5_1:-8772830 | Msa0715690:CDS |
| GCGGAGAAGGAGATAGAGAC+GGG | 0.509765 | 5_1:-8772594 | Msa0715690:CDS |
| GTCTCCATCATCCGGGCGAA+GGG | 0.529471 | 5_1:-8772829 | Msa0715690:CDS |
| GGATGATGGAGACGCTGTTA+CGG | 0.541392 | 5_1:+8772839 | None:intergenic |
| GGAACTAGGATTGGAGGAGG+CGG | 0.541402 | 5_1:-8772613 | Msa0715690:CDS |
| GAGTTTGGCACAGGCGATTG+TGG | 0.547955 | 5_1:-8772559 | Msa0715690:CDS |
| GCGTGTCGTGAAGCAATGGT+TGG | 0.550496 | 5_1:-8772954 | None:intergenic |
| GAAGGCGGAACTAGGATTGG+AGG | 0.553823 | 5_1:-8772619 | Msa0715690:CDS |
| GATTGTGGTGGCGGGAGCAG+AGG | 0.555003 | 5_1:-8772544 | Msa0715690:CDS |
| AAACAGCAACTCCCTTCGCC+CGG | 0.556955 | 5_1:+8772818 | None:intergenic |
| AAAAGCTGAGGAGATTGTGA+AGG | 0.558586 | 5_1:-8772694 | Msa0715690:CDS |
| GTCTACTGCTTTAACGGCGA+GGG | 0.561194 | 5_1:-8772784 | Msa0715690:CDS |
| CGTGTCGTGAAGCAATGGTT+GGG | 0.575227 | 5_1:-8772953 | None:intergenic |
| TTTGGCACAGGCGATTGTGG+TGG | 0.576692 | 5_1:-8772556 | Msa0715690:CDS |
| GGAGTTGCTGTTTCTCCTCA+CGG | 0.576809 | 5_1:-8772808 | Msa0715690:CDS |
| GGCGGAGAAGGAGATAGAGA+CGG | 0.579933 | 5_1:-8772595 | Msa0715690:CDS |
| CGCGGAGAAAGGATTGGAGA+CGG | 0.585103 | 5_1:-8772658 | Msa0715690:CDS |
| TAACAGCGTCTCCATCATCC+GGG | 0.601839 | 5_1:-8772836 | Msa0715690:CDS |
| GGCGGAACTAGGATTGGAGG+AGG | 0.605352 | 5_1:-8772616 | Msa0715690:CDS |
| CGGAGAAGGAGATAGAGACG+GGG | 0.636283 | 5_1:-8772593 | Msa0715690:CDS |
| CGTCTACTGCTTTAACGGCG+AGG | 0.652717 | 5_1:-8772785 | Msa0715690:CDS |
| GGAGAAAGGATTGGAGACGG+CGG | 0.673797 | 5_1:-8772655 | Msa0715690:CDS |
| CGTCTCCAATCCTTTCTCCG+CGG | 0.675587 | 5_1:+8772659 | None:intergenic |
| CTCTTCCGTATCCCGAACAG+AGG | 0.679487 | 5_1:+8772735 | None:intergenic |
| GGAGAAGGAGATAGAGACGG+GGG | 0.684188 | 5_1:-8772592 | Msa0715690:CDS |
| TTAAAGCAGTAGACGCCGTG+AGG | 0.691836 | 5_1:+8772793 | None:intergenic |
| GAAGGAAGGATTTGAATCCG+CGG | 0.697190 | 5_1:-8772676 | Msa0715690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATGTTGAAACGAGAGAGTT+GGG | + | chr5_1:8772505-8772524 | None:intergenic | 40.0% | |
| ACATGTTGAAACGAGAGAGT+TGG | + | chr5_1:8772506-8772525 | None:intergenic | 40.0% | |
| ATATTCCTCTGTTCGGGATA+CGG | - | chr5_1:8772675-8772694 | Msa0715690:CDS | 40.0% | |
| GAAAGCAGAAGAAAAAGCTG+AGG | - | chr5_1:8772709-8772728 | Msa0715690:CDS | 40.0% | |
| AAAAGCTGAGGAGATTGTGA+AGG | - | chr5_1:8772721-8772740 | Msa0715690:CDS | 40.0% | |
| !!! | AGCAGAGGTTTTTGGTCTTT+TGG | - | chr5_1:8772886-8772905 | Msa0715690:CDS | 40.0% |
| GCGCAAGATATTCCTCTGTT+CGG | - | chr5_1:8772668-8772687 | Msa0715690:CDS | 45.0% | |
| CGCAAGATATTCCTCTGTTC+GGG | - | chr5_1:8772669-8772688 | Msa0715690:CDS | 45.0% | |
| GAAGGAAGGATTTGAATCCG+CGG | - | chr5_1:8772739-8772758 | Msa0715690:CDS | 45.0% | |
| GGAAAAAGGACTTGAGAAGG+CGG | - | chr5_1:8772781-8772800 | Msa0715690:CDS | 45.0% | |
| !! | TGGTCTTTTGGTTGCTTCAG+CGG | - | chr5_1:8772898-8772917 | Msa0715690:CDS | 45.0% |
| ! | AGCGGTGGTGAATGGTATTT+TGG | - | chr5_1:8772916-8772935 | Msa0715690:CDS | 45.0% |
| ! | GCGGTGGTGAATGGTATTTT+GGG | - | chr5_1:8772917-8772936 | Msa0715690:CDS | 45.0% |
| ! | CGGTGGTGAATGGTATTTTG+GGG | - | chr5_1:8772918-8772937 | Msa0715690:CDS | 45.0% |
| !!! | AATGGTATTTTGGGGCCTGA+AGG | - | chr5_1:8772926-8772945 | Msa0715690:CDS | 45.0% |
| GCATGAAAACGACGTCGCAT+TGG | + | chr5_1:8772537-8772556 | None:intergenic | 50.0% | |
| !! | GGATGATGGAGACGCTGTTA+CGG | + | chr5_1:8772579-8772598 | None:intergenic | 50.0% |
| GTAACAGCGTCTCCATCATC+CGG | - | chr5_1:8772578-8772597 | Msa0715690:CDS | 50.0% | |
| TAACAGCGTCTCCATCATCC+GGG | - | chr5_1:8772579-8772598 | Msa0715690:CDS | 50.0% | |
| !! | GGAGTTGCTGTTTCTCCTCA+CGG | - | chr5_1:8772607-8772626 | Msa0715690:CDS | 50.0% |
| TTAAAGCAGTAGACGCCGTG+AGG | + | chr5_1:8772625-8772644 | None:intergenic | 50.0% | |
| CACGGCGTCTACTGCTTTAA+CGG | - | chr5_1:8772625-8772644 | Msa0715690:CDS | 50.0% | |
| GTCTACTGCTTTAACGGCGA+GGG | - | chr5_1:8772631-8772650 | Msa0715690:CDS | 50.0% | |
| GCTGAGGAGATTGTGAAGGA+AGG | - | chr5_1:8772725-8772744 | Msa0715690:CDS | 50.0% | |
| GGATTTGAATCCGCGGAGAA+AGG | - | chr5_1:8772746-8772765 | Msa0715690:CDS | 50.0% | |
| TGAATCCGCGGAGAAAGGAT+TGG | - | chr5_1:8772751-8772770 | Msa0715690:CDS | 50.0% | |
| GGCGGAAAAAGGACTTGAGA+AGG | - | chr5_1:8772778-8772797 | Msa0715690:CDS | 50.0% | |
| TGAGAAGGCGGAACTAGGAT+TGG | - | chr5_1:8772793-8772812 | Msa0715690:CDS | 50.0% | |
| !! | TCTTTTGGTTGCTTCAGCGG+TGG | - | chr5_1:8772901-8772920 | Msa0715690:CDS | 50.0% |
| AAACAGCAACTCCCTTCGCC+CGG | + | chr5_1:8772600-8772619 | None:intergenic | 55.0% | |
| CGTCTACTGCTTTAACGGCG+AGG | - | chr5_1:8772630-8772649 | Msa0715690:CDS | 55.0% | |
| GGCGAGGGAATCAGTGTCTA+TGG | - | chr5_1:8772646-8772665 | Msa0715690:CDS | 55.0% | |
| CTCTTCCGTATCCCGAACAG+AGG | + | chr5_1:8772683-8772702 | None:intergenic | 55.0% | |
| CGTCTCCAATCCTTTCTCCG+CGG | + | chr5_1:8772759-8772778 | None:intergenic | 55.0% | |
| CGCGGAGAAAGGATTGGAGA+CGG | - | chr5_1:8772757-8772776 | Msa0715690:CDS | 55.0% | |
| GGAGAAAGGATTGGAGACGG+CGG | - | chr5_1:8772760-8772779 | Msa0715690:CDS | 55.0% | |
| GGATTGGAGACGGCGGAAAA+AGG | - | chr5_1:8772767-8772786 | Msa0715690:CDS | 55.0% | |
| GGACTTGAGAAGGCGGAACT+AGG | - | chr5_1:8772788-8772807 | Msa0715690:CDS | 55.0% | |
| GAAGGCGGAACTAGGATTGG+AGG | - | chr5_1:8772796-8772815 | Msa0715690:CDS | 55.0% | |
| GGAACTAGGATTGGAGGAGG+CGG | - | chr5_1:8772802-8772821 | Msa0715690:CDS | 55.0% | |
| GGCGGAGAAGGAGATAGAGA+CGG | - | chr5_1:8772820-8772839 | Msa0715690:CDS | 55.0% | |
| GCGGAGAAGGAGATAGAGAC+GGG | - | chr5_1:8772821-8772840 | Msa0715690:CDS | 55.0% | |
| CGGAGAAGGAGATAGAGACG+GGG | - | chr5_1:8772822-8772841 | Msa0715690:CDS | 55.0% | |
| GGAGAAGGAGATAGAGACGG+GGG | - | chr5_1:8772823-8772842 | Msa0715690:CDS | 55.0% | |
| AAGGAGATAGAGACGGGGGT+GGG | - | chr5_1:8772827-8772846 | Msa0715690:CDS | 55.0% | |
| GAGTTTGGCACAGGCGATTG+TGG | - | chr5_1:8772856-8772875 | Msa0715690:CDS | 55.0% | |
| TTTGGCACAGGCGATTGTGG+TGG | - | chr5_1:8772859-8772878 | Msa0715690:CDS | 55.0% | |
| GTTGCTTCAGCGGTGGTGAA+TGG | - | chr5_1:8772908-8772927 | Msa0715690:CDS | 55.0% | |
| GTCTCCATCATCCGGGCGAA+GGG | - | chr5_1:8772586-8772605 | Msa0715690:CDS | 60.0% | |
| AACTCCCTTCGCCCGGATGA+TGG | + | chr5_1:8772593-8772612 | None:intergenic | 60.0% | |
| GGCGGAACTAGGATTGGAGG+AGG | - | chr5_1:8772799-8772818 | Msa0715690:CDS | 60.0% | |
| AGGATTGGAGGAGGCGGAGA+AGG | - | chr5_1:8772808-8772827 | Msa0715690:CDS | 60.0% | |
| GAAGGAGATAGAGACGGGGG+TGG | - | chr5_1:8772826-8772845 | Msa0715690:CDS | 60.0% | |
| AGGAGATAGAGACGGGGGTG+GGG | - | chr5_1:8772828-8772847 | Msa0715690:CDS | 60.0% | |
| !! | GTGGCGGGAGCAGAGGTTTT+TGG | - | chr5_1:8772878-8772897 | Msa0715690:CDS | 60.0% |
| CGTCTCCATCATCCGGGCGA+AGG | - | chr5_1:8772585-8772604 | Msa0715690:CDS | 65.0% | |
| ATAGAGACGGGGGTGGGGCT+TGG | - | chr5_1:8772833-8772852 | Msa0715690:CDS | 65.0% | |
| TAGAGACGGGGGTGGGGCTT+GGG | - | chr5_1:8772834-8772853 | Msa0715690:CDS | 65.0% | |
| GGGGCTTGGGAGTTTGGCAC+AGG | - | chr5_1:8772847-8772866 | Msa0715690:CDS | 65.0% | |
| GGCACAGGCGATTGTGGTGG+CGG | - | chr5_1:8772862-8772881 | Msa0715690:CDS | 65.0% | |
| GCACAGGCGATTGTGGTGGC+GGG | - | chr5_1:8772863-8772882 | Msa0715690:CDS | 65.0% | |
| !! | GATTGTGGTGGCGGGAGCAG+AGG | - | chr5_1:8772871-8772890 | Msa0715690:CDS | 65.0% |
| GGGGGTGGGGCTTGGGAGTT+TGG | - | chr5_1:8772841-8772860 | Msa0715690:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 8772476 | 8772961 | 8772476 | ID=Msa0715690;Name=Msa0715690 |
| chr5_1 | mRNA | 8772476 | 8772961 | 8772476 | ID=Msa0715690-mRNA-1;Parent=Msa0715690;Name=Msa0715690-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|161 |
| chr5_1 | exon | 8772476 | 8772961 | 8772476 | ID=Msa0715690-mRNA-1:exon:3601;Parent=Msa0715690-mRNA-1 |
| chr5_1 | CDS | 8772476 | 8772961 | 8772476 | ID=Msa0715690-mRNA-1:cds;Parent=Msa0715690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
