AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0723240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723240 MtrunA17_Chr5g0414091 95.402 435 20 0 1 435 1 435 0.0 849
Msa0723240 MtrunA17_Chr5g0414041 65.287 435 149 2 1 435 5 437 0.0 567
Msa0723240 MtrunA17_Chr5g0414061 62.212 434 158 3 2 435 15 442 0.0 548
Msa0723240 MtrunA17_Chr5g0414051 66.667 405 115 6 34 435 37 424 0.0 540
Msa0723240 MtrunA17_Chr3g0140371 66.482 361 111 5 75 433 10 362 2.05e-177 500
Msa0723240 MtrunA17_Chr8g0391481 63.612 371 127 5 66 432 7 373 1.10e-175 496
Msa0723240 MtrunA17_Chr7g0226991 61.972 355 128 4 82 433 54 404 4.12e-163 465
Msa0723240 MtrunA17_Chr5g0414081 70.149 201 56 3 236 433 2 201 3.88e-102 302
Msa0723240 MtrunA17_Chr8g0347321 36.854 426 253 8 14 431 22 439 4.54e-95 293
Msa0723240 MtrunA17_Chr8g0387921 41.477 352 195 7 85 431 74 419 5.30e-94 289
Msa0723240 MtrunA17_Chr6g0456691 37.349 415 242 10 25 432 17 420 7.76e-93 287
Msa0723240 MtrunA17_Chr6g0451321 38.172 372 212 8 66 433 102 459 2.93e-83 263
Msa0723240 MtrunA17_Chr6g0456681 38.275 371 215 7 68 432 47 409 8.54e-82 258
Msa0723240 MtrunA17_Chr2g0283851 37.921 356 196 12 90 433 60 402 5.37e-76 242
Msa0723240 MtrunA17_Chr1g0188191 33.609 363 214 11 85 433 234 583 1.15e-63 215
Msa0723240 MtrunA17_Chr6g0476041 32.540 378 202 13 83 433 165 516 3.01e-62 210
Msa0723240 MtrunA17_Chr7g0259711 31.956 363 216 12 84 433 90 434 1.60e-52 182
Msa0723240 MtrunA17_Chr7g0221281 35.656 244 145 6 193 432 2 237 5.09e-49 167
Msa0723240 MtrunA17_Chr2g0287901 33.423 371 203 14 84 435 96 441 8.34e-49 172
Msa0723240 MtrunA17_Chr1g0175671 31.967 366 209 14 85 433 170 512 1.22e-44 162
Msa0723240 MtrunA17_Chr4g0071391 31.806 371 200 14 83 431 107 446 3.12e-44 160
Msa0723240 MtrunA17_Chr7g0258851 29.976 417 238 17 43 433 202 590 8.48e-44 161
Msa0723240 MtrunA17_Chr1g0213281 30.663 362 207 15 84 426 174 510 4.51e-43 158
Msa0723240 MtrunA17_Chr4g0006991 30.053 376 213 16 79 431 79 427 1.50e-42 155
Msa0723240 MtrunA17_Chr4g0070341 29.973 367 219 12 76 427 124 467 4.96e-42 154
Msa0723240 MtrunA17_Chr8g0384151 31.373 357 209 12 83 431 88 416 8.04e-42 153
Msa0723240 MtrunA17_Chr7g0269991 30.704 355 206 14 85 427 137 463 3.14e-41 152
Msa0723240 MtrunA17_Chr4g0005491 31.111 360 213 16 85 427 62 403 3.30e-41 151
Msa0723240 MtrunA17_Chr4g0068111 27.842 431 244 18 27 431 64 453 1.30e-40 150
Msa0723240 MtrunA17_Chr5g0430371 29.570 372 215 10 77 427 50 395 1.36e-40 149
Msa0723240 MtrunA17_Chr7g0242701 31.347 386 213 16 69 431 71 427 1.66e-39 147
Msa0723240 MtrunA17_Chr5g0413271 28.099 363 226 12 81 433 141 478 4.73e-39 147
Msa0723240 MtrunA17_Chr7g0243301 30.495 364 210 16 85 427 64 405 8.95e-39 144
Msa0723240 MtrunA17_Chr8g0360521 26.744 430 254 16 23 427 12 405 1.05e-38 144
Msa0723240 MtrunA17_Chr3g0091031 29.167 360 196 10 83 427 29 344 2.15e-38 142
Msa0723240 MtrunA17_Chr3g0105301 29.630 351 224 11 82 427 40 372 2.63e-38 142
Msa0723240 MtrunA17_Chr2g0314801 31.549 355 207 12 82 431 30 353 3.21e-38 141
Msa0723240 MtrunA17_Chr8g0368461 28.900 391 221 17 73 432 166 530 4.23e-38 145
Msa0723240 MtrunA17_Chr3g0126021 29.897 388 211 14 69 433 113 462 7.07e-37 140
Msa0723240 MtrunA17_Chr7g0221271 34.392 189 114 4 29 212 22 205 1.98e-36 132
Msa0723240 MtrunA17_Chr3g0085081 29.363 361 194 14 83 427 29 344 4.53e-36 135
Msa0723240 MtrunA17_Chr6g0455901 28.125 448 263 18 8 431 4 416 5.53e-36 137
Msa0723240 MtrunA17_Chr3g0113671 28.056 360 228 13 82 427 103 445 2.60e-35 135
Msa0723240 MtrunA17_Chr2g0314791 29.330 358 207 14 82 431 26 345 3.69e-35 133
Msa0723240 MtrunA17_Chr3g0085071 29.143 350 209 11 83 427 44 359 3.99e-35 133
Msa0723240 MtrunA17_Chr8g0384421 26.203 374 219 11 83 431 131 472 7.27e-35 135
Msa0723240 MtrunA17_Chr3g0097891 30.387 362 206 14 76 427 21 346 1.53e-34 131
Msa0723240 MtrunA17_Chr4g0049421 27.934 426 238 17 41 431 1 392 3.44e-34 132
Msa0723240 MtrunA17_Chr1g0178081 28.099 363 178 12 83 433 53 344 4.77e-34 130
Msa0723240 MtrunA17_Chr2g0325481 30.925 346 203 11 85 427 50 362 8.36e-34 130
Msa0723240 MtrunA17_Chr4g0045941 30.029 343 207 10 84 424 32 343 1.98e-33 128
Msa0723240 MtrunA17_Chr5g0437601 27.078 373 213 14 82 427 80 420 5.23e-33 129
Msa0723240 MtrunA17_Chr3g0114101 28.022 364 215 13 85 427 89 426 1.90e-32 127
Msa0723240 MtrunA17_Chr4g0041011 28.941 387 239 13 49 431 9 363 2.88e-32 125
Msa0723240 MtrunA17_Chr4g0045951 28.986 345 208 10 84 424 44 355 3.20e-32 125
Msa0723240 MtrunA17_Chr1g0171171 27.297 370 233 11 67 431 37 375 3.72e-32 125
Msa0723240 MtrunA17_Chr1g0210771 26.891 357 220 13 80 427 54 378 4.42e-31 122
Msa0723240 MtrunA17_Chr2g0283581 29.235 366 219 14 70 431 29 358 5.09e-31 122
Msa0723240 MtrunA17_Chr5g0437291 27.624 362 228 13 83 427 163 507 1.00e-30 124
Msa0723240 MtrunA17_Chr5g0413851 29.915 351 205 13 83 427 40 355 2.74e-30 120
Msa0723240 MtrunA17_Chr4g0005481 27.612 402 240 17 51 431 75 446 8.24e-29 117
Msa0723240 MtrunA17_Chr7g0221291 36.424 151 87 3 29 174 22 168 2.61e-28 109
Msa0723240 MtrunA17_Chr1g0209921 31.667 180 113 6 79 255 38 210 1.12e-19 88.6
Msa0723240 MtrunA17_Chr4g0057171 43.158 95 48 2 227 321 1 89 8.91e-19 82.0
Msa0723240 MtrunA17_Chr7g0275501 57.377 61 23 1 370 430 11 68 2.59e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723240 AT1G70230.1 48.113 424 188 8 27 435 9 415 1.63e-148 429
Msa0723240 AT5G15890.1 39.535 387 215 8 50 432 152 523 7.50e-98 303
Msa0723240 AT4G01080.1 38.539 397 223 7 35 429 51 428 2.42e-91 283
Msa0723240 AT4G23790.1 36.449 428 259 9 10 431 7 427 1.76e-90 281
Msa0723240 AT3G02440.1 38.764 356 197 7 85 432 127 469 4.85e-90 281
Msa0723240 AT3G02440.2 38.764 356 197 7 85 432 135 477 9.61e-90 281
Msa0723240 AT1G01430.1 38.624 378 217 8 61 431 78 447 2.65e-89 279
Msa0723240 AT3G28150.1 36.790 405 238 10 36 433 19 412 3.34e-89 277
Msa0723240 AT4G11090.1 36.343 432 254 12 9 431 6 425 7.02e-88 274
Msa0723240 AT5G15900.1 36.675 379 222 10 65 435 45 413 9.78e-81 256
Msa0723240 AT5G15900.2 36.675 379 222 10 65 435 64 432 1.44e-80 256
Msa0723240 AT4G25360.1 33.668 398 233 12 51 433 145 526 4.55e-69 228
Msa0723240 AT4G25360.2 33.668 398 233 12 51 433 145 526 4.55e-69 228
Msa0723240 AT5G51640.1 33.333 393 223 14 63 433 115 490 2.16e-65 218
Msa0723240 AT5G19160.1 34.332 367 210 11 84 435 98 448 9.03e-57 194
Msa0723240 AT3G06080.2 31.680 363 220 10 85 435 103 449 3.92e-52 182
Msa0723240 AT3G12060.1 32.249 369 207 15 77 427 188 531 1.74e-50 179
Msa0723240 AT5G06700.1 32.418 364 214 15 83 433 253 597 3.69e-46 168
Msa0723240 AT5G64020.1 30.811 370 215 14 85 431 57 408 9.41e-46 163
Msa0723240 AT1G48880.1 33.245 376 199 16 71 428 95 436 5.17e-45 162
Msa0723240 AT3G11570.1 31.143 350 194 14 101 431 98 419 4.32e-43 157
Msa0723240 AT2G14530.1 29.048 420 237 19 36 431 24 406 1.20e-42 155
Msa0723240 AT5G20590.1 31.250 368 215 13 75 427 131 475 2.60e-42 156
Msa0723240 AT5G49340.1 29.296 355 224 11 84 427 99 437 1.34e-40 150
Msa0723240 AT5G58600.1 28.796 382 218 11 68 431 55 400 4.37e-40 148
Msa0723240 AT3G14850.2 32.312 359 205 11 79 431 28 354 7.28e-40 146
Msa0723240 AT3G62390.1 28.532 361 226 13 83 433 136 474 7.74e-40 149
Msa0723240 AT5G06230.2 30.458 371 211 15 83 431 19 364 3.53e-39 145
Msa0723240 AT5G06230.1 30.458 371 211 15 83 431 60 405 5.92e-39 145
Msa0723240 AT2G31110.2 29.444 360 218 13 72 427 31 358 7.72e-38 141
Msa0723240 AT2G38320.1 28.453 362 230 12 80 427 54 400 9.38e-38 142
Msa0723240 AT2G30010.1 30.556 360 220 10 84 431 55 396 2.19e-37 140
Msa0723240 AT5G20680.6 27.204 397 245 11 57 431 83 457 2.89e-37 141
Msa0723240 AT2G30010.2 31.025 361 217 12 84 431 98 439 3.16e-37 141
Msa0723240 AT2G42570.1 31.285 358 195 15 85 431 47 364 3.72e-37 139
Msa0723240 AT5G19160.2 35.628 247 140 8 84 320 98 335 8.28e-37 138
Msa0723240 AT2G37720.1 29.076 368 216 12 83 431 141 482 1.01e-36 140
Msa0723240 AT5G20680.2 27.500 400 240 12 57 431 159 533 1.10e-36 141
Msa0723240 AT5G20680.4 27.500 400 240 12 57 431 177 551 1.13e-36 141
Msa0723240 AT5G20680.1 27.500 400 240 12 57 431 177 551 1.13e-36 141
Msa0723240 AT5G20680.3 27.500 400 240 12 57 431 177 551 1.13e-36 141
Msa0723240 AT5G20680.5 27.500 400 240 12 57 431 177 551 1.13e-36 141
Msa0723240 AT2G30900.1 29.261 352 213 11 82 427 40 361 3.29e-36 137
Msa0723240 AT1G73140.1 29.032 372 222 15 72 427 56 401 1.06e-35 136
Msa0723240 AT5G64470.3 27.855 359 228 12 85 431 55 394 3.34e-35 135
Msa0723240 AT1G60790.1 28.075 374 218 16 83 432 188 534 8.46e-35 136
Msa0723240 AT3G11030.1 27.374 358 230 11 83 427 104 444 2.44e-34 133
Msa0723240 AT2G34070.1 28.125 352 217 12 84 431 64 383 4.22e-34 131
Msa0723240 AT5G01360.2 27.701 361 227 13 83 428 34 375 5.30e-34 131
Msa0723240 AT1G29050.1 28.333 360 223 12 75 431 51 378 5.66e-34 131
Msa0723240 AT2G38320.2 28.834 326 204 11 115 427 7 317 6.42e-34 129
Msa0723240 AT5G64470.2 27.577 359 235 12 85 431 55 400 7.48e-34 131
Msa0723240 AT5G01360.1 27.701 361 227 13 83 428 90 431 2.00e-33 130
Msa0723240 AT2G40320.1 28.022 364 221 13 82 427 78 418 2.10e-33 130
Msa0723240 AT3G55990.1 28.177 362 223 13 83 427 138 479 3.04e-33 131
Msa0723240 AT2G40160.1 27.123 365 222 14 85 427 77 419 1.05e-32 128
Msa0723240 AT2G40160.2 27.123 365 222 14 85 427 77 419 1.32e-32 128
Msa0723240 AT3G06080.1 31.579 247 150 6 85 321 103 340 2.70e-32 125
Msa0723240 AT2G40150.1 28.165 387 232 16 63 427 54 416 4.75e-32 126
Msa0723240 AT1G78710.1 29.640 361 216 14 76 431 29 356 5.01e-31 122
Msa0723240 AT3G54260.1 27.222 360 213 14 85 431 51 374 3.42e-30 120
Msa0723240 AT5G01620.2 25.946 370 237 13 77 431 102 449 8.10e-28 115
Msa0723240 AT5G01620.1 25.946 370 237 13 77 431 102 449 8.10e-28 115
Msa0723240 AT5G01620.3 25.946 370 237 13 77 431 110 457 9.33e-28 115
Msa0723240 AT3G62390.2 28.455 246 163 6 83 322 136 374 2.74e-27 113
Msa0723240 AT5G20590.2 34.392 189 115 5 75 255 131 318 1.90e-26 110
Msa0723240 AT5G58600.2 30.038 263 145 8 68 319 55 289 1.86e-25 105
Msa0723240 AT5G06230.3 30.769 260 145 11 83 321 60 305 2.99e-24 102
Msa0723240 AT2G31110.3 32.609 184 116 4 72 254 31 207 4.23e-24 101
Msa0723240 AT2G40160.3 35.878 131 82 2 85 214 77 206 4.35e-23 99.4
Msa0723240 AT2G30900.2 33.714 175 109 3 82 255 40 208 1.31e-22 97.4
Msa0723240 AT5G64470.4 27.376 263 171 9 85 335 55 309 2.76e-19 88.6
Msa0723240 AT5G64470.1 27.376 263 171 9 85 335 55 309 3.78e-19 88.6
Msa0723240 AT3G14850.1 29.787 282 160 11 156 431 2 251 1.52e-18 85.5
Msa0723240 AT1G78710.2 31.667 180 114 4 76 254 22 193 2.95e-18 85.5
Msa0723240 AT2G34070.2 26.449 276 167 12 156 427 2 245 7.16e-16 77.4

Find 102 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTCAATTATACTATTA+AGG 0.109514 5_1:+20833259 None:intergenic
GTGCCACTTACGAAATTTAT+TGG 0.120096 5_1:+20833575 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5_1:-20833776 Msa0723240:CDS
GTCCTCAAAGCCTTTGTTAA+TGG 0.201729 5_1:+20833285 None:intergenic
TATGAGAGTGATTTAGTTCT+TGG 0.213205 5_1:-20833357 Msa0723240:CDS
GCTTAGAAAGGGTTTGAAAA+TGG 0.214590 5_1:-20834336 Msa0723240:exon
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5_1:+20833646 None:intergenic
ACAAATGGTAGACCTGATTT+AGG 0.287971 5_1:-20833789 Msa0723240:CDS
AGGTATTAGACTTGAGGTTT+TGG 0.334346 5_1:-20833031 Msa0723240:CDS
TCTCTCAATTATACTATTAA+GGG 0.345275 5_1:+20833260 None:intergenic
GTGAATATTCAAATGGAGAT+TGG 0.348520 5_1:-20833884 Msa0723240:CDS
CTTAGAAAGGGTTTGAAAAT+GGG 0.349836 5_1:-20834335 Msa0723240:exon
AAAGTGTTTGGTTCAAATCT+TGG 0.349855 5_1:+20833726 None:intergenic
TCAACACCATGCACTAGAAA+TGG 0.356439 5_1:+20833513 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5_1:-20833104 Msa0723240:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5_1:-20833240 Msa0723240:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5_1:+20834236 None:intergenic
GACATGATTGTTATATCAAC+CGG 0.390990 5_1:-20833408 Msa0723240:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5_1:+20833844 None:intergenic
AGAAAAGAGGATAAGATAGT+TGG 0.399914 5_1:-20833129 Msa0723240:CDS
ATTTATTGGACTTGCCATCT+TGG 0.403569 5_1:+20833589 None:intergenic
CACCATAAAACAGTGATTAG+AGG 0.408129 5_1:-20834370 Msa0723240:five_prime_UTR
TTCCATTAACAAAGGCTTTG+AGG 0.412649 5_1:-20833287 Msa0723240:CDS
CAACACCATGCACTAGAAAT+GGG 0.415330 5_1:+20833514 None:intergenic
TACAAAATGATTGTGTTCAT+TGG 0.415725 5_1:-20832918 Msa0723240:CDS
TGTGTTCATTGGTGCTTGCC+AGG 0.419306 5_1:-20832907 Msa0723240:CDS
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5_1:+20833603 None:intergenic
GTTTGTTCTTAACGAGTTTG+AGG 0.427143 5_1:+20833703 None:intergenic
GGCAAAGGATATTGATCAAA+TGG 0.427874 5_1:-20833430 Msa0723240:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5_1:+20832946 None:intergenic
TGAATATTCAAATGGAGATT+GGG 0.433426 5_1:-20833883 Msa0723240:CDS
TGAATAGTGGTAGGTGGAAA+TGG 0.434406 5_1:+20834157 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5_1:+20833369 None:intergenic
GGATAAGATAGTTGGAGGAA+TGG 0.436305 5_1:-20833121 Msa0723240:CDS
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5_1:+20832952 None:intergenic
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5_1:+20834144 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5_1:+20833843 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5_1:+20833777 None:intergenic
CTTGGTAGATTACACTCACT+TGG 0.451639 5_1:+20833744 None:intergenic
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5_1:-20833770 Msa0723240:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5_1:+20832982 None:intergenic
AGCAGCTTAGTTGGTGCAAA+AGG 0.466236 5_1:+20834191 None:intergenic
ACGAGTTTGAGGAAAGTGTT+TGG 0.469541 5_1:+20833714 None:intergenic
TAGTTGGTGCAAAAGGAGTA+AGG 0.469996 5_1:+20834198 None:intergenic
AAAGAGGATAAGATAGTTGG+AGG 0.471238 5_1:-20833126 Msa0723240:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5_1:-20832988 Msa0723240:CDS
TGTAGAGATGATGAAGAAGT+GGG 0.476890 5_1:-20832857 Msa0723240:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5_1:-20833519 Msa0723240:CDS
TGATGAAGTATAAACAAAGA+AGG 0.490126 5_1:+20834228 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.491810 5_1:-20833142 Msa0723240:CDS
GTAAAAGCTAATAAATTTGA+AGG 0.492014 5_1:-20833051 Msa0723240:CDS
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5_1:-20833603 Msa0723240:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5_1:+20832964 None:intergenic
AAATCATGTGAATATTCAAA+TGG 0.499467 5_1:-20833891 Msa0723240:CDS
AAGATAGTTGGAGGAATGGA+TGG 0.499482 5_1:-20833117 Msa0723240:CDS
GTAGAATGAAGCAGCTTAGT+TGG 0.499755 5_1:+20834182 None:intergenic
TTGTAGAGATGATGAAGAAG+TGG 0.501696 5_1:-20832858 Msa0723240:CDS
TGCCAGGACCTATAGATACT+TGG 0.502047 5_1:-20832891 Msa0723240:CDS
GGAGGAAAGATTGTCATTGA+AGG 0.503097 5_1:+20834301 None:intergenic
CATGAAAGAAAGCTTAGAAA+GGG 0.505195 5_1:-20834347 Msa0723240:five_prime_UTR
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5_1:+20834148 None:intergenic
AGTGAGAGATGCATCACAAA+TGG 0.510170 5_1:-20833804 Msa0723240:CDS
TTGTTGGAGATTCTTTGGCT+AGG 0.514286 5_1:-20833671 Msa0723240:CDS
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5_1:-20833246 Msa0723240:CDS
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5_1:-20833177 Msa0723240:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5_1:-20833498 Msa0723240:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.524335 5_1:+20832883 None:intergenic
GAGGAAAGATTGTCATTGAA+GGG 0.526115 5_1:+20834302 None:intergenic
GCATGAAAGAAAGCTTAGAA+AGG 0.526820 5_1:-20834348 Msa0723240:five_prime_UTR
GATGAAGTATAAACAAAGAA+GGG 0.527867 5_1:+20834229 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5_1:-20833578 Msa0723240:CDS
TTGTTATATCAACCGGGCAT+TGG 0.533327 5_1:-20833401 Msa0723240:CDS
GAGTCATGAAAGGAAGAGAA+AGG 0.534479 5_1:+20834425 None:intergenic
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5_1:+20832889 None:intergenic
ATTTGAAGGTATTAGACTTG+AGG 0.537007 5_1:-20833037 Msa0723240:CDS
AAGGATTGTGATGGAAGAAG+TGG 0.538045 5_1:-20833085 Msa0723240:CDS
ATACCTACAAAAGAAGTTGA+AGG 0.538773 5_1:+20834116 None:intergenic
GGATCTTGTCAATGAGAAGT+GGG 0.539423 5_1:-20833451 Msa0723240:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5_1:+20833189 None:intergenic
TTCTTTGATTGTGCCACATG+TGG 0.561553 5_1:+20833827 None:intergenic
TGGATCTTGTCAATGAGAAG+TGG 0.565608 5_1:-20833452 Msa0723240:CDS
GAAAATGTAGCATTATGAGA+AGG 0.570303 5_1:+20833549 None:intergenic
AGGAAAGATTGTCATTGAAG+GGG 0.571923 5_1:+20834303 None:intergenic
ATTTGAGTATGATTAAGCTC+AGG 0.573371 5_1:+20833321 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.577718 5_1:+20834151 None:intergenic
ACTTGCCATCTTGGTAAACA+AGG 0.587524 5_1:+20833598 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5_1:-20832997 Msa0723240:CDS
AAAGGATTCATGTAAGGACC+TGG 0.594011 5_1:+20832970 None:intergenic
CTTTATGAGAGAGTCATGAA+AGG 0.600156 5_1:+20834415 None:intergenic
GAGTAACATCAACATAGAGA+AGG 0.616784 5_1:-20832835 Msa0723240:exon
TGTCAATGAGAAGTGGGCAA+AGG 0.618169 5_1:-20833445 Msa0723240:CDS
AGTAACATCAACATAGAGAA+GGG 0.625804 5_1:-20832834 Msa0723240:exon
TGAGATTAGAAGGATTGTGA+TGG 0.626027 5_1:-20833094 Msa0723240:CDS
ATATACAATGTCACCACATG+TGG 0.627135 5_1:-20833840 Msa0723240:CDS
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5_1:-20833497 Msa0723240:CDS
AGTTGGTGCAAAAGGAGTAA+GGG 0.640399 5_1:+20834199 None:intergenic
GGAAGAAGTGGAAAGTGCCA+AGG 0.647334 5_1:-20833073 Msa0723240:CDS
CCTACAAAAGAAGTTGAAGG+AGG 0.659746 5_1:+20834119 None:intergenic
AACACCATGCACTAGAAATG+GGG 0.662494 5_1:+20833515 None:intergenic
ACATGATTGTTATATCAACC+GGG 0.662677 5_1:-20833407 Msa0723240:CDS
GATTGGGTTAGTGACATGAG+AGG 0.690038 5_1:-20833867 Msa0723240:CDS
ATAGTGGTAGGTGGAAATGG+TGG 0.690039 5_1:+20834160 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5_1:20833791-20833810 Msa0723240:CDS 10.0%
!!! TTATTATAATACAATGTTTT+TGG - chr5_1:20833791-20833810 Msa0723240:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5_1:20832986-20833005 None:intergenic 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5_1:20833324-20833343 Msa0723240:CDS 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5_1:20833325-20833344 Msa0723240:CDS 15.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5_1:20832986-20833005 None:intergenic 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5_1:20833324-20833343 Msa0723240:CDS 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5_1:20833325-20833344 Msa0723240:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5_1:20832983-20833002 None:intergenic 20.0%
!! AAATCATGTGAATATTCAAA+TGG - chr5_1:20833372-20833391 Msa0723240:CDS 20.0%
!!! TTTTTATGTTCCATTAACAA+AGG - chr5_1:20833968-20833987 Msa0723240:intron 20.0%
!! TCTCTCAATTATACTATTAA+GGG + chr5_1:20834006-20834025 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTA+AGG + chr5_1:20834007-20834026 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTACCT+TGG + chr5_1:20834210-20834229 None:intergenic 20.0%
!! GTAAAAGCTAATAAATTTGA+AGG - chr5_1:20834212-20834231 Msa0723240:CDS 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5_1:20834338-20834357 None:intergenic 20.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5_1:20832983-20833002 None:intergenic 20.0%
!! AAATCATGTGAATATTCAAA+TGG - chr5_1:20833372-20833391 Msa0723240:CDS 20.0%
!!! TTTTTATGTTCCATTAACAA+AGG - chr5_1:20833968-20833987 Msa0723240:intron 20.0%
!! TCTCTCAATTATACTATTAA+GGG + chr5_1:20834006-20834025 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTA+AGG + chr5_1:20834007-20834026 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTACCT+TGG + chr5_1:20834210-20834229 None:intergenic 20.0%
!! GTAAAAGCTAATAAATTTGA+AGG - chr5_1:20834212-20834231 Msa0723240:CDS 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5_1:20834338-20834357 None:intergenic 20.0%
! TGTTGAGACAAAAAATCAAT+GGG - chr5_1:20832812-20832831 Msa0723240:three_prime_UTR 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5_1:20833030-20833049 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5_1:20833037-20833056 None:intergenic 25.0%
! TGATGAAGTATAAACAAAGA+AGG + chr5_1:20833038-20833057 None:intergenic 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5_1:20833281-20833300 Msa0723240:CDS 25.0%
! TGAATATTCAAATGGAGATT+GGG - chr5_1:20833380-20833399 Msa0723240:CDS 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5_1:20833489-20833508 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5_1:20833487-20833506 Msa0723240:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5_1:20833576-20833595 Msa0723240:CDS 25.0%
!! ATGCTACATTTTCTTTGTAT+TGG - chr5_1:20833724-20833743 Msa0723240:CDS 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5_1:20834131-20834150 None:intergenic 25.0%
! TACAAAATGATTGTGTTCAT+TGG - chr5_1:20834345-20834364 Msa0723240:five_prime_UTR 25.0%
! TGTTGAGACAAAAAATCAAT+GGG - chr5_1:20832812-20832831 Msa0723240:three_prime_UTR 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5_1:20833030-20833049 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5_1:20833037-20833056 None:intergenic 25.0%
! TGATGAAGTATAAACAAAGA+AGG + chr5_1:20833038-20833057 None:intergenic 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5_1:20833281-20833300 Msa0723240:CDS 25.0%
! TGAATATTCAAATGGAGATT+GGG - chr5_1:20833380-20833399 Msa0723240:CDS 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5_1:20833489-20833508 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5_1:20833487-20833506 Msa0723240:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5_1:20833576-20833595 Msa0723240:CDS 25.0%
!! ATGCTACATTTTCTTTGTAT+TGG - chr5_1:20833724-20833743 Msa0723240:CDS 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5_1:20834131-20834150 None:intergenic 25.0%
! TACAAAATGATTGTGTTCAT+TGG - chr5_1:20834345-20834364 Msa0723240:five_prime_UTR 25.0%
GTGTTGAGACAAAAAATCAA+TGG - chr5_1:20832811-20832830 Msa0723240:three_prime_UTR 30.0%
CATGAAAGAAAGCTTAGAAA+GGG - chr5_1:20832916-20832935 Msa0723240:CDS 30.0%
CTTAGAAAGGGTTTGAAAAT+GGG - chr5_1:20832928-20832947 Msa0723240:CDS 30.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5_1:20833122-20833141 None:intergenic 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5_1:20833150-20833169 None:intergenic 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5_1:20833290-20833309 Msa0723240:CDS 30.0%
GTGAATATTCAAATGGAGAT+TGG - chr5_1:20833379-20833398 Msa0723240:CDS 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5_1:20833422-20833441 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5_1:20833493-20833512 Msa0723240:CDS 30.0%
! AAAGTGTTTGGTTCAAATCT+TGG + chr5_1:20833540-20833559 None:intergenic 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5_1:20833685-20833704 Msa0723240:CDS 30.0%
GAAAATGTAGCATTATGAGA+AGG + chr5_1:20833717-20833736 None:intergenic 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5_1:20833766-20833785 Msa0723240:CDS 30.0%
GACATGATTGTTATATCAAC+CGG - chr5_1:20833855-20833874 Msa0723240:CDS 30.0%
ACATGATTGTTATATCAACC+GGG - chr5_1:20833856-20833875 Msa0723240:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5_1:20833897-20833916 None:intergenic 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG - chr5_1:20833906-20833925 Msa0723240:CDS 30.0%
ATTTGAGTATGATTAAGCTC+AGG + chr5_1:20833945-20833964 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5_1:20834017-20834036 Msa0723240:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5_1:20834023-20834042 Msa0723240:intron 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5_1:20834134-20834153 Msa0723240:CDS 30.0%
!! ATTTGAAGGTATTAGACTTG+AGG - chr5_1:20834226-20834245 Msa0723240:CDS 30.0%
!!! GGTTTTGGATGTAACTAAAT+TGG - chr5_1:20834247-20834266 Msa0723240:CDS 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5_1:20834302-20834321 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5_1:20834308-20834327 Msa0723240:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5_1:20834383-20834402 None:intergenic 30.0%
AGTAACATCAACATAGAGAA+GGG - chr5_1:20834429-20834448 Msa0723240:five_prime_UTR 30.0%
GTGTTGAGACAAAAAATCAA+TGG - chr5_1:20832811-20832830 Msa0723240:three_prime_UTR 30.0%
CATGAAAGAAAGCTTAGAAA+GGG - chr5_1:20832916-20832935 Msa0723240:CDS 30.0%
CTTAGAAAGGGTTTGAAAAT+GGG - chr5_1:20832928-20832947 Msa0723240:CDS 30.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5_1:20833122-20833141 None:intergenic 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5_1:20833150-20833169 None:intergenic 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5_1:20833290-20833309 Msa0723240:CDS 30.0%
GTGAATATTCAAATGGAGAT+TGG - chr5_1:20833379-20833398 Msa0723240:CDS 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5_1:20833422-20833441 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5_1:20833493-20833512 Msa0723240:CDS 30.0%
! AAAGTGTTTGGTTCAAATCT+TGG + chr5_1:20833540-20833559 None:intergenic 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5_1:20833685-20833704 Msa0723240:CDS 30.0%
GAAAATGTAGCATTATGAGA+AGG + chr5_1:20833717-20833736 None:intergenic 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5_1:20833766-20833785 Msa0723240:CDS 30.0%
GACATGATTGTTATATCAAC+CGG - chr5_1:20833855-20833874 Msa0723240:CDS 30.0%
ACATGATTGTTATATCAACC+GGG - chr5_1:20833856-20833875 Msa0723240:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5_1:20833897-20833916 None:intergenic 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG - chr5_1:20833906-20833925 Msa0723240:CDS 30.0%
ATTTGAGTATGATTAAGCTC+AGG + chr5_1:20833945-20833964 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5_1:20834017-20834036 Msa0723240:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5_1:20834023-20834042 Msa0723240:intron 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5_1:20834134-20834153 Msa0723240:CDS 30.0%
!! ATTTGAAGGTATTAGACTTG+AGG - chr5_1:20834226-20834245 Msa0723240:CDS 30.0%
!!! GGTTTTGGATGTAACTAAAT+TGG - chr5_1:20834247-20834266 Msa0723240:CDS 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5_1:20834302-20834321 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5_1:20834308-20834327 Msa0723240:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5_1:20834383-20834402 None:intergenic 30.0%
AGTAACATCAACATAGAGAA+GGG - chr5_1:20834429-20834448 Msa0723240:five_prime_UTR 30.0%
CTTTATGAGAGAGTCATGAA+AGG + chr5_1:20832851-20832870 None:intergenic 35.0%
!!! TCACTGTTTTATGGTGTTGT+CGG + chr5_1:20832889-20832908 None:intergenic 35.0%
CACCATAAAACAGTGATTAG+AGG - chr5_1:20832893-20832912 Msa0723240:CDS 35.0%
! TGCCTCTAATCACTGTTTTA+TGG + chr5_1:20832898-20832917 None:intergenic 35.0%
GCATGAAAGAAAGCTTAGAA+AGG - chr5_1:20832915-20832934 Msa0723240:CDS 35.0%
GCTTAGAAAGGGTTTGAAAA+TGG - chr5_1:20832927-20832946 Msa0723240:CDS 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5_1:20832963-20832982 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5_1:20832964-20832983 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5_1:20833118-20833137 None:intergenic 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5_1:20833335-20833354 Msa0723240:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5_1:20833423-20833442 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG - chr5_1:20833423-20833442 Msa0723240:CDS 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5_1:20833474-20833493 Msa0723240:CDS 35.0%
GTTTGTTCTTAACGAGTTTG+AGG + chr5_1:20833563-20833582 None:intergenic 35.0%
! AGCTTTTGTTGGAGATTCTT+TGG - chr5_1:20833587-20833606 Msa0723240:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5_1:20833620-20833639 None:intergenic 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5_1:20833677-20833696 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5_1:20833691-20833710 None:intergenic 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5_1:20833765-20833784 Msa0723240:CDS 35.0%
GGCAAAGGATATTGATCAAA+TGG - chr5_1:20833833-20833852 Msa0723240:CDS 35.0%
TTCCATTAACAAAGGCTTTG+AGG - chr5_1:20833976-20833995 Msa0723240:intron 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5_1:20834068-20834087 Msa0723240:intron 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5_1:20834076-20834095 Msa0723240:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5_1:20834082-20834101 Msa0723240:intron 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5_1:20834121-20834140 Msa0723240:CDS 35.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5_1:20834137-20834156 Msa0723240:CDS 35.0%
TGAGATTAGAAGGATTGTGA+TGG - chr5_1:20834169-20834188 Msa0723240:CDS 35.0%
! AGGTATTAGACTTGAGGTTT+TGG - chr5_1:20834232-20834251 Msa0723240:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5_1:20834314-20834333 None:intergenic 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5_1:20834320-20834339 None:intergenic 35.0%
TTGTAGAGATGATGAAGAAG+TGG - chr5_1:20834405-20834424 Msa0723240:five_prime_UTR 35.0%
TGTAGAGATGATGAAGAAGT+GGG - chr5_1:20834406-20834425 Msa0723240:five_prime_UTR 35.0%
GAGTAACATCAACATAGAGA+AGG - chr5_1:20834428-20834447 Msa0723240:five_prime_UTR 35.0%
CTTTATGAGAGAGTCATGAA+AGG + chr5_1:20832851-20832870 None:intergenic 35.0%
!!! TCACTGTTTTATGGTGTTGT+CGG + chr5_1:20832889-20832908 None:intergenic 35.0%
CACCATAAAACAGTGATTAG+AGG - chr5_1:20832893-20832912 Msa0723240:CDS 35.0%
! TGCCTCTAATCACTGTTTTA+TGG + chr5_1:20832898-20832917 None:intergenic 35.0%
GCATGAAAGAAAGCTTAGAA+AGG - chr5_1:20832915-20832934 Msa0723240:CDS 35.0%
GCTTAGAAAGGGTTTGAAAA+TGG - chr5_1:20832927-20832946 Msa0723240:CDS 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5_1:20832963-20832982 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5_1:20832964-20832983 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5_1:20833118-20833137 None:intergenic 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5_1:20833335-20833354 Msa0723240:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5_1:20833423-20833442 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG - chr5_1:20833423-20833442 Msa0723240:CDS 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5_1:20833474-20833493 Msa0723240:CDS 35.0%
GTTTGTTCTTAACGAGTTTG+AGG + chr5_1:20833563-20833582 None:intergenic 35.0%
! AGCTTTTGTTGGAGATTCTT+TGG - chr5_1:20833587-20833606 Msa0723240:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5_1:20833620-20833639 None:intergenic 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5_1:20833677-20833696 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5_1:20833691-20833710 None:intergenic 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5_1:20833765-20833784 Msa0723240:CDS 35.0%
GGCAAAGGATATTGATCAAA+TGG - chr5_1:20833833-20833852 Msa0723240:CDS 35.0%
TTCCATTAACAAAGGCTTTG+AGG - chr5_1:20833976-20833995 Msa0723240:intron 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5_1:20834068-20834087 Msa0723240:intron 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5_1:20834076-20834095 Msa0723240:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5_1:20834082-20834101 Msa0723240:intron 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5_1:20834121-20834140 Msa0723240:CDS 35.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5_1:20834137-20834156 Msa0723240:CDS 35.0%
TGAGATTAGAAGGATTGTGA+TGG - chr5_1:20834169-20834188 Msa0723240:CDS 35.0%
! AGGTATTAGACTTGAGGTTT+TGG - chr5_1:20834232-20834251 Msa0723240:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5_1:20834314-20834333 None:intergenic 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5_1:20834320-20834339 None:intergenic 35.0%
TTGTAGAGATGATGAAGAAG+TGG - chr5_1:20834405-20834424 Msa0723240:five_prime_UTR 35.0%
TGTAGAGATGATGAAGAAGT+GGG - chr5_1:20834406-20834425 Msa0723240:five_prime_UTR 35.0%
GAGTAACATCAACATAGAGA+AGG - chr5_1:20834428-20834447 Msa0723240:five_prime_UTR 35.0%
GAGTCATGAAAGGAAGAGAA+AGG + chr5_1:20832841-20832860 None:intergenic 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5_1:20832965-20832984 None:intergenic 40.0%
! AGTTGGTGCAAAAGGAGTAA+GGG + chr5_1:20833067-20833086 None:intergenic 40.0%
! TAGTTGGTGCAAAAGGAGTA+AGG + chr5_1:20833068-20833087 None:intergenic 40.0%
GTAGAATGAAGCAGCTTAGT+TGG + chr5_1:20833084-20833103 None:intergenic 40.0%
TGAATAGTGGTAGGTGGAAA+TGG + chr5_1:20833109-20833128 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5_1:20833115-20833134 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5_1:20833147-20833166 None:intergenic 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5_1:20833144-20833163 Msa0723240:CDS 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5_1:20833439-20833458 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5_1:20833459-20833478 Msa0723240:CDS 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5_1:20833522-20833541 None:intergenic 40.0%
!! ACGAGTTTGAGGAAAGTGTT+TGG + chr5_1:20833552-20833571 None:intergenic 40.0%
TTGTTGGAGATTCTTTGGCT+AGG - chr5_1:20833592-20833611 Msa0723240:CDS 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5_1:20833663-20833682 None:intergenic 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5_1:20833660-20833679 Msa0723240:CDS 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5_1:20833668-20833687 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5_1:20833751-20833770 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5_1:20833752-20833771 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5_1:20833753-20833772 None:intergenic 40.0%
TGGATCTTGTCAATGAGAAG+TGG - chr5_1:20833811-20833830 Msa0723240:CDS 40.0%
GGATCTTGTCAATGAGAAGT+GGG - chr5_1:20833812-20833831 Msa0723240:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5_1:20833862-20833881 Msa0723240:CDS 40.0%
GTCCTCAAAGCCTTTGTTAA+TGG + chr5_1:20833981-20834000 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5_1:20834077-20834096 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5_1:20834075-20834094 Msa0723240:intron 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5_1:20834142-20834161 Msa0723240:CDS 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5_1:20834146-20834165 Msa0723240:CDS 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5_1:20834159-20834178 Msa0723240:CDS 40.0%
! AAGGATTGTGATGGAAGAAG+TGG - chr5_1:20834178-20834197 Msa0723240:CDS 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5_1:20834266-20834285 Msa0723240:CDS 40.0%
AAAGGATTCATGTAAGGACC+TGG + chr5_1:20834296-20834315 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5_1:20834377-20834396 None:intergenic 40.0%
GAGTCATGAAAGGAAGAGAA+AGG + chr5_1:20832841-20832860 None:intergenic 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5_1:20832965-20832984 None:intergenic 40.0%
! AGTTGGTGCAAAAGGAGTAA+GGG + chr5_1:20833067-20833086 None:intergenic 40.0%
! TAGTTGGTGCAAAAGGAGTA+AGG + chr5_1:20833068-20833087 None:intergenic 40.0%
GTAGAATGAAGCAGCTTAGT+TGG + chr5_1:20833084-20833103 None:intergenic 40.0%
TGAATAGTGGTAGGTGGAAA+TGG + chr5_1:20833109-20833128 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5_1:20833115-20833134 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5_1:20833147-20833166 None:intergenic 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5_1:20833144-20833163 Msa0723240:CDS 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5_1:20833439-20833458 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5_1:20833459-20833478 Msa0723240:CDS 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5_1:20833522-20833541 None:intergenic 40.0%
!! ACGAGTTTGAGGAAAGTGTT+TGG + chr5_1:20833552-20833571 None:intergenic 40.0%
TTGTTGGAGATTCTTTGGCT+AGG - chr5_1:20833592-20833611 Msa0723240:CDS 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5_1:20833663-20833682 None:intergenic 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5_1:20833660-20833679 Msa0723240:CDS 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5_1:20833668-20833687 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5_1:20833751-20833770 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5_1:20833752-20833771 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5_1:20833753-20833772 None:intergenic 40.0%
TGGATCTTGTCAATGAGAAG+TGG - chr5_1:20833811-20833830 Msa0723240:CDS 40.0%
GGATCTTGTCAATGAGAAGT+GGG - chr5_1:20833812-20833831 Msa0723240:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5_1:20833862-20833881 Msa0723240:CDS 40.0%
GTCCTCAAAGCCTTTGTTAA+TGG + chr5_1:20833981-20834000 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5_1:20834077-20834096 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5_1:20834075-20834094 Msa0723240:intron 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5_1:20834142-20834161 Msa0723240:CDS 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5_1:20834146-20834165 Msa0723240:CDS 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5_1:20834159-20834178 Msa0723240:CDS 40.0%
! AAGGATTGTGATGGAAGAAG+TGG - chr5_1:20834178-20834197 Msa0723240:CDS 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5_1:20834266-20834285 Msa0723240:CDS 40.0%
AAAGGATTCATGTAAGGACC+TGG + chr5_1:20834296-20834315 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5_1:20834377-20834396 None:intergenic 40.0%
! AGCAGCTTAGTTGGTGCAAA+AGG + chr5_1:20833075-20833094 None:intergenic 45.0%
ATAGTGGTAGGTGGAAATGG+TGG + chr5_1:20833106-20833125 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5_1:20833396-20833415 Msa0723240:CDS 45.0%
TGTCAATGAGAAGTGGGCAA+AGG - chr5_1:20833818-20833837 Msa0723240:CDS 45.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5_1:20833877-20833896 None:intergenic 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5_1:20834372-20834391 Msa0723240:five_prime_UTR 45.0%
! AGCAGCTTAGTTGGTGCAAA+AGG + chr5_1:20833075-20833094 None:intergenic 45.0%
ATAGTGGTAGGTGGAAATGG+TGG + chr5_1:20833106-20833125 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5_1:20833396-20833415 Msa0723240:CDS 45.0%
TGTCAATGAGAAGTGGGCAA+AGG - chr5_1:20833818-20833837 Msa0723240:CDS 45.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5_1:20833877-20833896 None:intergenic 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5_1:20834372-20834391 Msa0723240:five_prime_UTR 45.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5_1:20833744-20833763 Msa0723240:CDS 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5_1:20834086-20834105 Msa0723240:intron 50.0%
!! GGAAGAAGTGGAAAGTGCCA+AGG - chr5_1:20834190-20834209 Msa0723240:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5_1:20834275-20834294 Msa0723240:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5_1:20834284-20834303 None:intergenic 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG - chr5_1:20834356-20834375 Msa0723240:five_prime_UTR 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5_1:20833744-20833763 Msa0723240:CDS 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5_1:20834086-20834105 Msa0723240:intron 50.0%
!! GGAAGAAGTGGAAAGTGCCA+AGG - chr5_1:20834190-20834209 Msa0723240:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5_1:20834275-20834294 Msa0723240:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5_1:20834284-20834303 None:intergenic 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG - chr5_1:20834356-20834375 Msa0723240:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20832807 20834478 20832807 ID=Msa0723240;Name=Msa0723240
chr5_1 mRNA 20832807 20834478 20832807 ID=Msa0723240-mRNA-1;Parent=Msa0723240;Name=Msa0723240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=139|1|1|1|1|1|2|45|435
chr5_1 exon 20832807 20833939 20832807 ID=Msa0723240-mRNA-1:exon:7955;Parent=Msa0723240-mRNA-1
chr5_1 exon 20834120 20834478 20834120 ID=Msa0723240-mRNA-1:exon:7954;Parent=Msa0723240-mRNA-1
chr5_1 five_prime_UTR 20834340 20834478 20834340 ID=Msa0723240-mRNA-1:five_prime_utr;Parent=Msa0723240-mRNA-1
chr5_1 CDS 20834120 20834339 20834120 ID=Msa0723240-mRNA-1:cds;Parent=Msa0723240-mRNA-1
chr5_1 CDS 20832852 20833939 20832852 ID=Msa0723240-mRNA-1:cds;Parent=Msa0723240-mRNA-1
chr5_1 three_prime_UTR 20832807 20832851 20832807 ID=Msa0723240-mRNA-1:three_prime_utr;Parent=Msa0723240-mRNA-1
Gene Sequence

>Msa0723240

ATGGGAAGCACAAACCCCTTCAATGACAATCTTTCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCATTTTGTCCCTTACTCCTTTTGCACCAACTAAGCTGCTTCATTCTACCACCATTTCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGAATATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAAAGTGAGAGATGCATCACAAATGGTAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGATTTGAACCAAACACTTTCCTCAAACTCGTTAAGAACAAACATATAGCTTTTGTTGGAGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTAGCGACTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCCTTCTCATAATGCTACATTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAAGTGGGCAAAGGATATTGATCAAATGGACATGATTGTTATATCAACCGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAGTTCTTGGTAGCTTGAATTGTCCTGAGCTTAATCATACTCAAATTGATTTTTATGTTCCATTAACAAAGGCTTTGAGGACTACCCTTAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGATAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGTGATGGAAGAAGTGGAAAGTGCCAAGGTAAAAGCTAATAAATTTGAAGGTATTAGACTTGAGGTTTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAATGATTGTGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTTTTGTAGAGATGATGAAGAAGTGGGAGTAA
Protein sequence

>Msa0723240

MGSTNPFNDNLSSFNKKFFHLTLYSLLPISLLCLYFIILSLTPFAPTKLLHSTTISTYHYSSTYSSPPSTSFVGKNKVYDYEKSCEYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPNTFLKLVKNKHIAFVGDSLARNQLESLLCMLATTSTPNLVYQDGKSNKFRKWHFPSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNEKWAKDIDQMDMIVISTGHWFLLPSIYYESDLVLGSLNCPELNHTQIDFYVPLTKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEDKIVGGMDGEIRRIVMEEVESAKVKANKFEGIRLEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFVEMMKKWE*