AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0843400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843400 MtrunA17_Chr5g0414091 97.241 435 12 0 1 435 1 435 0.0 885
Msa0843400 MtrunA17_Chr5g0414041 65.287 435 149 2 1 435 5 437 0.0 569
Msa0843400 MtrunA17_Chr5g0414061 62.212 434 158 3 2 435 15 442 0.0 548
Msa0843400 MtrunA17_Chr5g0414051 66.914 405 114 6 34 435 37 424 0.0 540
Msa0843400 MtrunA17_Chr3g0140371 67.036 361 109 5 75 433 10 362 4.30e-178 501
Msa0843400 MtrunA17_Chr8g0391481 63.881 371 126 5 66 432 7 373 2.30e-175 495
Msa0843400 MtrunA17_Chr7g0226991 61.690 355 129 4 82 433 54 404 5.85e-162 462
Msa0843400 MtrunA17_Chr5g0414081 71.642 201 53 3 236 433 2 201 3.64e-105 310
Msa0843400 MtrunA17_Chr8g0347321 37.089 426 252 8 14 431 22 439 5.73e-96 295
Msa0843400 MtrunA17_Chr8g0387921 41.761 352 194 7 85 431 74 419 5.27e-95 292
Msa0843400 MtrunA17_Chr6g0456691 37.108 415 243 10 25 432 17 420 2.30e-92 285
Msa0843400 MtrunA17_Chr6g0456681 38.275 371 215 7 68 432 47 409 1.56e-83 262
Msa0843400 MtrunA17_Chr6g0451321 37.903 372 213 8 66 433 102 459 2.06e-82 261
Msa0843400 MtrunA17_Chr2g0283851 37.360 356 198 12 90 433 60 402 5.49e-76 242
Msa0843400 MtrunA17_Chr1g0188191 33.333 363 215 11 85 433 234 583 9.95e-65 218
Msa0843400 MtrunA17_Chr6g0476041 32.804 378 201 13 83 433 165 516 2.88e-63 213
Msa0843400 MtrunA17_Chr7g0259711 33.333 369 203 15 84 433 90 434 2.99e-52 181
Msa0843400 MtrunA17_Chr2g0287901 33.423 371 203 14 84 435 96 441 1.28e-49 174
Msa0843400 MtrunA17_Chr7g0221281 35.656 244 145 6 193 432 2 237 7.36e-49 167
Msa0843400 MtrunA17_Chr1g0175671 32.787 366 206 14 85 433 170 512 3.22e-47 169
Msa0843400 MtrunA17_Chr7g0258851 31.566 415 234 17 43 433 202 590 6.12e-46 167
Msa0843400 MtrunA17_Chr4g0071391 31.967 366 206 14 83 431 107 446 1.85e-45 163
Msa0843400 MtrunA17_Chr7g0269991 31.564 358 199 14 85 427 137 463 5.26e-43 157
Msa0843400 MtrunA17_Chr1g0213281 30.663 362 207 14 84 426 174 510 5.87e-43 158
Msa0843400 MtrunA17_Chr5g0430371 30.110 362 226 10 77 427 50 395 6.92e-42 152
Msa0843400 MtrunA17_Chr8g0384151 29.692 357 215 10 83 431 88 416 1.27e-41 152
Msa0843400 MtrunA17_Chr4g0006991 29.521 376 215 16 79 431 79 427 2.75e-41 151
Msa0843400 MtrunA17_Chr4g0070341 29.973 367 219 12 76 427 124 467 4.36e-41 152
Msa0843400 MtrunA17_Chr4g0068111 27.594 424 254 17 27 431 64 453 7.61e-41 151
Msa0843400 MtrunA17_Chr4g0005491 30.556 360 215 16 85 427 62 403 2.86e-40 148
Msa0843400 MtrunA17_Chr7g0242701 31.088 386 214 15 69 431 71 427 6.78e-40 148
Msa0843400 MtrunA17_Chr3g0091031 29.885 348 209 11 83 427 29 344 3.67e-39 144
Msa0843400 MtrunA17_Chr8g0368461 29.381 388 223 16 73 432 166 530 4.17e-39 147
Msa0843400 MtrunA17_Chr3g0105301 30.086 349 225 11 82 427 40 372 4.87e-39 144
Msa0843400 MtrunA17_Chr8g0360521 26.588 425 261 15 23 427 12 405 8.32e-39 144
Msa0843400 MtrunA17_Chr7g0243301 30.495 364 210 16 85 427 64 405 1.06e-38 144
Msa0843400 MtrunA17_Chr5g0413271 28.412 359 230 12 81 433 141 478 2.26e-38 145
Msa0843400 MtrunA17_Chr2g0314801 30.986 355 209 13 82 431 30 353 6.44e-38 140
Msa0843400 MtrunA17_Chr6g0455901 28.251 446 265 16 8 431 4 416 2.57e-37 140
Msa0843400 MtrunA17_Chr3g0085081 30.199 351 204 14 83 427 29 344 5.20e-37 138
Msa0843400 MtrunA17_Chr3g0126021 28.866 388 215 14 69 433 113 462 2.30e-36 139
Msa0843400 MtrunA17_Chr3g0113671 27.500 360 230 12 82 427 103 445 1.62e-35 136
Msa0843400 MtrunA17_Chr3g0097891 30.663 362 205 14 76 427 21 346 4.43e-35 133
Msa0843400 MtrunA17_Chr7g0221271 33.862 189 115 4 29 212 22 205 5.24e-35 129
Msa0843400 MtrunA17_Chr3g0085071 29.799 349 208 12 83 427 44 359 5.69e-35 133
Msa0843400 MtrunA17_Chr4g0045941 30.029 343 207 10 84 424 32 343 5.88e-35 132
Msa0843400 MtrunA17_Chr8g0384421 26.722 363 231 9 83 431 131 472 7.27e-35 135
Msa0843400 MtrunA17_Chr2g0314791 30.447 358 203 14 82 431 26 345 1.38e-34 131
Msa0843400 MtrunA17_Chr1g0178081 28.846 364 174 13 83 433 53 344 5.44e-34 130
Msa0843400 MtrunA17_Chr1g0171171 27.989 368 233 12 67 431 37 375 1.27e-33 129
Msa0843400 MtrunA17_Chr5g0437601 29.111 371 208 13 82 427 80 420 1.48e-33 130
Msa0843400 MtrunA17_Chr4g0045951 30.029 343 207 10 84 424 44 355 2.41e-33 129
Msa0843400 MtrunA17_Chr4g0049421 27.934 426 238 17 41 431 1 392 3.97e-33 129
Msa0843400 MtrunA17_Chr2g0325481 30.347 346 205 11 85 427 50 362 1.06e-32 127
Msa0843400 MtrunA17_Chr3g0114101 28.889 360 217 12 85 427 89 426 1.31e-32 128
Msa0843400 MtrunA17_Chr1g0210771 26.230 366 211 13 80 427 54 378 2.98e-32 126
Msa0843400 MtrunA17_Chr2g0283581 29.315 365 220 14 70 431 29 358 1.72e-31 123
Msa0843400 MtrunA17_Chr4g0041011 28.535 389 238 15 49 431 9 363 3.75e-31 122
Msa0843400 MtrunA17_Chr5g0413851 29.915 351 205 13 83 427 40 355 1.20e-30 121
Msa0843400 MtrunA17_Chr4g0005481 27.500 400 243 16 51 431 75 446 1.77e-29 119
Msa0843400 MtrunA17_Chr5g0437291 26.923 364 228 13 83 427 163 507 1.87e-29 120
Msa0843400 MtrunA17_Chr7g0221291 36.667 150 86 3 29 173 22 167 1.46e-28 110
Msa0843400 MtrunA17_Chr1g0209921 33.333 186 114 6 73 255 32 210 1.05e-21 94.4
Msa0843400 MtrunA17_Chr4g0057171 43.158 95 48 2 227 321 1 89 5.33e-19 82.8
Msa0843400 MtrunA17_Chr7g0275501 55.738 61 24 1 370 430 11 68 5.33e-18 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843400 AT1G70230.1 48.821 424 185 8 27 435 9 415 2.04e-150 434
Msa0843400 AT5G15890.1 39.276 387 216 8 50 432 152 523 1.84e-98 305
Msa0843400 AT4G01080.1 38.945 398 220 8 35 429 51 428 5.49e-91 283
Msa0843400 AT1G01430.1 39.418 378 214 8 61 431 78 447 9.07e-91 282
Msa0843400 AT3G02440.1 38.764 356 197 7 85 432 127 469 2.21e-90 282
Msa0843400 AT3G02440.2 38.764 356 197 7 85 432 135 477 3.74e-90 282
Msa0843400 AT4G23790.1 36.682 428 258 9 10 431 7 427 8.68e-90 279
Msa0843400 AT3G28150.1 36.790 405 238 10 36 433 19 412 6.73e-89 276
Msa0843400 AT4G11090.1 36.806 432 252 12 9 431 6 425 4.02e-88 275
Msa0843400 AT5G15900.1 36.675 379 222 10 65 435 45 413 1.40e-81 258
Msa0843400 AT5G15900.2 36.675 379 222 10 65 435 64 432 1.85e-81 258
Msa0843400 AT4G25360.1 33.753 397 234 11 51 433 145 526 1.78e-70 232
Msa0843400 AT4G25360.2 33.753 397 234 11 51 433 145 526 1.78e-70 232
Msa0843400 AT5G51640.1 35.135 370 208 13 82 433 135 490 6.86e-66 219
Msa0843400 AT5G19160.1 35.422 367 206 13 84 435 98 448 2.79e-55 190
Msa0843400 AT3G06080.2 32.507 363 217 11 85 435 103 449 1.75e-51 180
Msa0843400 AT3G12060.1 32.181 376 211 16 77 433 188 538 8.23e-50 177
Msa0843400 AT5G06700.1 31.593 364 217 14 83 433 253 597 6.29e-46 168
Msa0843400 AT1G48880.1 34.043 376 196 17 71 428 95 436 1.16e-45 164
Msa0843400 AT5G64020.1 31.081 370 214 14 85 431 57 408 2.65e-45 162
Msa0843400 AT5G20590.1 31.250 368 215 13 75 427 131 475 2.69e-43 159
Msa0843400 AT3G11570.1 29.954 434 238 17 20 431 30 419 1.01e-42 155
Msa0843400 AT2G14530.1 29.286 420 236 19 36 431 24 406 1.46e-42 155
Msa0843400 AT5G58600.1 29.839 372 227 11 68 431 55 400 2.75e-41 151
Msa0843400 AT3G62390.1 28.997 369 214 14 83 433 136 474 4.41e-41 152
Msa0843400 AT5G49340.1 29.640 361 215 12 84 427 99 437 6.55e-40 149
Msa0843400 AT3G14850.2 32.312 359 205 11 79 431 28 354 6.92e-40 146
Msa0843400 AT2G30010.1 29.630 378 200 10 84 431 55 396 3.01e-39 145
Msa0843400 AT2G30010.2 30.079 379 197 12 84 431 98 439 5.83e-39 146
Msa0843400 AT2G31110.2 29.444 360 218 13 72 427 31 358 4.35e-38 142
Msa0843400 AT2G38320.1 28.453 362 230 12 80 427 54 400 8.39e-38 142
Msa0843400 AT5G06230.2 30.053 376 206 16 83 431 19 364 9.34e-38 141
Msa0843400 AT5G06230.1 30.585 376 204 17 83 431 60 405 1.41e-37 141
Msa0843400 AT1G60790.1 27.717 368 227 13 83 432 188 534 6.06e-37 142
Msa0843400 AT5G19160.2 36.992 246 138 8 84 320 98 335 1.45e-36 137
Msa0843400 AT5G01360.2 28.532 361 224 13 83 428 34 375 3.67e-36 137
Msa0843400 AT5G01360.1 28.532 361 224 13 83 428 90 431 1.23e-35 137
Msa0843400 AT2G37720.1 28.804 368 217 12 83 431 141 482 1.93e-35 137
Msa0843400 AT5G20680.6 26.700 397 247 12 57 431 83 457 2.40e-35 136
Msa0843400 AT2G42570.1 30.618 356 200 16 85 431 47 364 2.76e-35 134
Msa0843400 AT1G73140.1 29.223 373 220 17 72 427 56 401 6.43e-35 134
Msa0843400 AT5G20680.2 26.700 397 247 12 57 431 159 533 7.28e-35 136
Msa0843400 AT5G20680.4 26.700 397 247 12 57 431 177 551 7.44e-35 136
Msa0843400 AT5G20680.1 26.700 397 247 12 57 431 177 551 7.44e-35 136
Msa0843400 AT5G20680.3 26.700 397 247 12 57 431 177 551 7.44e-35 136
Msa0843400 AT5G20680.5 26.700 397 247 12 57 431 177 551 7.44e-35 136
Msa0843400 AT5G64470.3 28.611 360 224 14 85 431 55 394 1.05e-34 133
Msa0843400 AT3G11030.1 28.056 360 225 12 83 427 104 444 1.12e-34 134
Msa0843400 AT2G30900.1 28.693 352 215 11 82 427 40 361 5.35e-34 130
Msa0843400 AT2G40320.1 28.689 366 216 13 82 427 78 418 5.39e-34 132
Msa0843400 AT2G38320.2 28.834 326 204 11 115 427 7 317 6.42e-34 129
Msa0843400 AT3G55990.1 28.532 361 223 14 83 427 138 479 1.09e-33 132
Msa0843400 AT2G40160.1 28.022 364 220 15 85 427 77 419 2.34e-33 130
Msa0843400 AT5G64470.2 28.333 360 231 14 85 431 55 400 2.46e-33 130
Msa0843400 AT2G40160.2 27.123 365 222 14 85 427 77 419 2.73e-33 130
Msa0843400 AT1G29050.1 28.333 360 223 12 75 431 51 378 4.88e-33 128
Msa0843400 AT2G34070.1 27.841 352 218 12 84 431 64 383 1.29e-32 127
Msa0843400 AT3G54260.1 27.778 360 211 13 85 431 51 374 2.08e-32 127
Msa0843400 AT2G40150.1 28.052 385 235 16 63 427 54 416 1.28e-31 125
Msa0843400 AT3G06080.1 31.984 247 149 6 85 321 103 340 1.41e-31 124
Msa0843400 AT3G62390.2 29.268 246 161 6 83 322 136 374 4.22e-29 118
Msa0843400 AT1G78710.1 30.387 362 212 16 76 431 29 356 4.69e-29 117
Msa0843400 AT5G20590.2 31.418 261 152 10 75 321 131 378 8.99e-28 114
Msa0843400 AT5G01620.2 25.867 375 231 13 77 431 102 449 3.69e-27 113
Msa0843400 AT5G01620.1 25.867 375 231 13 77 431 102 449 3.69e-27 113
Msa0843400 AT5G01620.3 25.867 375 231 13 77 431 110 457 3.85e-27 113
Msa0843400 AT5G58600.2 31.621 253 154 8 68 319 55 289 9.45e-27 109
Msa0843400 AT2G31110.3 33.152 184 115 4 72 254 31 207 1.99e-24 102
Msa0843400 AT5G06230.3 30.916 262 142 11 83 321 60 305 1.76e-23 100
Msa0843400 AT2G40160.3 36.641 131 81 2 85 214 77 206 3.09e-23 100
Msa0843400 AT2G30900.2 33.143 175 110 3 82 255 40 208 4.49e-21 92.8
Msa0843400 AT5G64470.4 28.030 264 168 11 85 335 55 309 8.90e-19 87.0
Msa0843400 AT5G64470.1 28.030 264 168 11 85 335 55 309 9.14e-19 87.4
Msa0843400 AT3G14850.1 29.787 282 160 11 156 431 2 251 1.13e-18 85.5
Msa0843400 AT1G78710.2 31.111 180 115 4 76 254 22 193 4.84e-17 81.6
Msa0843400 AT2G34070.2 26.449 276 167 12 156 427 2 245 4.61e-15 75.1

Find 104 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCCACTTACGAAATTTAT+TGG 0.120096 5_4:+21062050 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5_4:-21062251 Msa0843400:CDS
TTCTCTCAATTATACTATTT+AGG 0.139860 5_4:+21061734 None:intergenic
GTCCTCAAAGCCTTTCTTAA+TGG 0.190045 5_4:+21061760 None:intergenic
TATGAGAGTGATTTAGTTCT+TGG 0.213205 5_4:-21061832 Msa0843400:CDS
GCTTAGAAAGGGTTTGAAAA+TGG 0.214590 5_4:-21062811 Msa0843400:exon
TCTCTCAATTATACTATTTA+GGG 0.267875 5_4:+21061735 None:intergenic
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5_4:+21062121 None:intergenic
ACAAATGGTAGACCTGATTT+AGG 0.287971 5_4:-21062264 Msa0843400:CDS
CTTAGAAAGGGTTTGAAAAT+GGG 0.349836 5_4:-21062810 Msa0843400:exon
TTGTAGAGATGATGAAGAAA+TGG 0.352065 5_4:-21061333 Msa0843400:CDS
TCAACACCATGCACTAGAAA+TGG 0.356439 5_4:+21061988 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5_4:-21061579 Msa0843400:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5_4:-21061715 Msa0843400:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5_4:+21062711 None:intergenic
GTGACTATTCAAATGGAGAT+TGG 0.369081 5_4:-21062359 Msa0843400:CDS
GGTGTTGGATGTAACTAAAT+TGG 0.388499 5_4:-21061491 Msa0843400:CDS
GACATGATTGTTATATCAAC+CGG 0.390990 5_4:-21061883 Msa0843400:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5_4:+21062319 None:intergenic
AGAAAAGAGGATAAGATAGT+TGG 0.399914 5_4:-21061604 Msa0843400:CDS
ATTTATTGGACTTGCCATCT+TGG 0.403569 5_4:+21062064 None:intergenic
TGTAGAGATGATGAAGAAAT+GGG 0.404562 5_4:-21061332 Msa0843400:CDS
CACCATAAAACAGTGATTAG+AGG 0.408129 5_4:-21062845 Msa0843400:five_prime_UTR
CAACACCATGCACTAGAAAT+GGG 0.415330 5_4:+21061989 None:intergenic
TACAAAATGATTGTGTTCAT+TGG 0.415725 5_4:-21061393 Msa0843400:CDS
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5_4:+21062078 None:intergenic
TTCCATTAAGAAAGGCTTTG+AGG 0.431515 5_4:-21061762 Msa0843400:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5_4:+21061421 None:intergenic
TGAATAGTGGTAGGTGGAAA+TGG 0.434406 5_4:+21062632 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5_4:+21061844 None:intergenic
GGATAAGATAGTTGGAGGAA+TGG 0.436305 5_4:-21061596 Msa0843400:CDS
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5_4:+21061427 None:intergenic
GGCCAAGGATATTGATCAAA+TGG 0.439901 5_4:-21061905 Msa0843400:CDS
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5_4:+21062619 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5_4:+21062318 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5_4:+21062252 None:intergenic
TGTGTTCATTGGTGTTTGCC+AGG 0.446360 5_4:-21061382 Msa0843400:CDS
ACAAAAGCTAATAAATTTGA+AGG 0.447815 5_4:-21061526 Msa0843400:CDS
GTTTGTTCTTAATGAGATTG+AGG 0.450023 5_4:+21062178 None:intergenic
CTTGGTAGATTACACTCACT+TGG 0.451639 5_4:+21062219 None:intergenic
TGACTATTCAAATGGAGATT+GGG 0.453167 5_4:-21062358 Msa0843400:CDS
ATTTGAAGGTATTAGAATTG+AGG 0.457268 5_4:-21061512 Msa0843400:CDS
AAGGATTGAGATTGAAGAAG+TGG 0.457929 5_4:-21061560 Msa0843400:CDS
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5_4:-21062245 Msa0843400:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5_4:+21061457 None:intergenic
AGCAGCTTAGTTGGTGCAAA+AGG 0.466236 5_4:+21062666 None:intergenic
TAGTTGGTGCAAAAGGAGTA+AGG 0.469996 5_4:+21062673 None:intergenic
AAAGAGGATAAGATAGTTGG+AGG 0.471238 5_4:-21061601 Msa0843400:CDS
AAATCATGTGACTATTCAAA+TGG 0.471379 5_4:-21062366 Msa0843400:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5_4:-21061463 Msa0843400:CDS
TGCCAGGACCTATAGATACT+TGG 0.476712 5_4:-21061366 Msa0843400:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5_4:-21061994 Msa0843400:CDS
TGATGAAGTATAAACAAAGA+AGG 0.490126 5_4:+21062703 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.491810 5_4:-21061617 Msa0843400:CDS
GTCCATTTGATCAATATCCT+TGG 0.493677 5_4:+21061903 None:intergenic
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5_4:-21062078 Msa0843400:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5_4:+21061439 None:intergenic
AAGATAGTTGGAGGAATGGA+TGG 0.499482 5_4:-21061592 Msa0843400:CDS
GTAGAATGAAGCAGCTTAGT+TGG 0.499755 5_4:+21062657 None:intergenic
GGAGGAAAGATTGTCATTGA+AGG 0.503097 5_4:+21062776 None:intergenic
CATGAAAGAAAGCTTAGAAA+GGG 0.505195 5_4:-21062822 Msa0843400:five_prime_UTR
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5_4:+21062623 None:intergenic
TGGATCTTGTCAATGAGAGG+TGG 0.507379 5_4:-21061927 Msa0843400:CDS
AGTGAGAGATGCATCACAAA+TGG 0.510170 5_4:-21062279 Msa0843400:CDS
TTGTTGGGGATTCTTTGGCT+AGG 0.510878 5_4:-21062146 Msa0843400:CDS
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5_4:-21061721 Msa0843400:CDS
AAAGTGCTTGGTTCAAACCT+TGG 0.519498 5_4:+21062201 None:intergenic
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5_4:-21061652 Msa0843400:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5_4:-21061973 Msa0843400:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.524335 5_4:+21061358 None:intergenic
GAGGAAAGATTGTCATTGAA+GGG 0.526115 5_4:+21062777 None:intergenic
TGGGGACCAATACAAAGAAA+AGG 0.526383 5_4:+21062008 None:intergenic
GCATGAAAGAAAGCTTAGAA+AGG 0.526820 5_4:-21062823 Msa0843400:five_prime_UTR
GATGAAGTATAAACAAAGAA+GGG 0.527867 5_4:+21062704 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5_4:-21062053 Msa0843400:CDS
TTGTTATATCAACCGGGCAT+TGG 0.533327 5_4:-21061876 Msa0843400:CDS
GAGTCATGAAAGGAAGAGAA+AGG 0.534479 5_4:+21062900 None:intergenic
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5_4:+21061364 None:intergenic
ATACCTACAAAAGAAGTTGA+AGG 0.538773 5_4:+21062591 None:intergenic
GGATCTTGTCAATGAGAGGT+GGG 0.546338 5_4:-21061926 Msa0843400:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5_4:+21061664 None:intergenic
ATGAGATTGAGGAAAGTGCT+TGG 0.551052 5_4:+21062189 None:intergenic
TTCTTTGATTGTGCCACATG+TGG 0.561553 5_4:+21062302 None:intergenic
AGGTATTAGAATTGAGGTGT+TGG 0.565513 5_4:-21061506 Msa0843400:CDS
AGGAAAGATTGTCATTGAAG+GGG 0.571923 5_4:+21062778 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.577718 5_4:+21062626 None:intergenic
AATTTGAGTATGATTAAACT+CGG 0.579926 5_4:+21061795 None:intergenic
TGTCAATGAGAGGTGGGCCA+AGG 0.581180 5_4:-21061920 Msa0843400:CDS
ACTTGCCATCTTGGTAAACA+AGG 0.587524 5_4:+21062073 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5_4:-21061472 Msa0843400:CDS
AAAGGATTCATGTAAGGACC+TGG 0.594011 5_4:+21061445 None:intergenic
CTTTATGAGAGAGTCATGAA+AGG 0.600156 5_4:+21062890 None:intergenic
GAGTAACATCAACATAGAGA+AGG 0.606973 5_4:-21061310 Msa0843400:exon
AGTAACATCAACATAGAGAA+GGG 0.625804 5_4:-21061309 Msa0843400:exon
ATATACAATGTCACCACATG+TGG 0.627135 5_4:-21062315 Msa0843400:CDS
ATTTGAGTATGATTAAACTC+GGG 0.631629 5_4:+21061796 None:intergenic
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5_4:-21061972 Msa0843400:CDS
AGTTGGTGCAAAAGGAGTAA+GGG 0.640399 5_4:+21062674 None:intergenic
CAAGTGAGTGTAATCTACCA+AGG 0.650297 5_4:-21062218 Msa0843400:CDS
CCTACAAAAGAAGTTGAAGG+AGG 0.659746 5_4:+21062594 None:intergenic
AACACCATGCACTAGAAATG+GGG 0.662494 5_4:+21061990 None:intergenic
ACATGATTGTTATATCAACC+GGG 0.662677 5_4:-21061882 Msa0843400:CDS
GATTGGGTTAGTGACATGAG+AGG 0.690038 5_4:-21062342 Msa0843400:CDS
ATAGTGGTAGGTGGAAATGG+TGG 0.690039 5_4:+21062635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5_4:21062266-21062285 Msa0843400:CDS 10.0%
!!! TTATTATAATACAATGTTTT+TGG - chr5_4:21062266-21062285 Msa0843400:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5_4:21061461-21061480 None:intergenic 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5_4:21061799-21061818 Msa0843400:CDS 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5_4:21061800-21061819 Msa0843400:CDS 15.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5_4:21061461-21061480 None:intergenic 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5_4:21061799-21061818 Msa0843400:CDS 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5_4:21061800-21061819 Msa0843400:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5_4:21061458-21061477 None:intergenic 20.0%
!! AATTTGAGTATGATTAAACT+CGG + chr5_4:21062421-21062440 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5_4:21062443-21062462 Msa0843400:intron 20.0%
!! TCTCTCAATTATACTATTTA+GGG + chr5_4:21062481-21062500 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTT+AGG + chr5_4:21062482-21062501 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG + chr5_4:21062685-21062704 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG - chr5_4:21062687-21062706 Msa0843400:CDS 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5_4:21062813-21062832 None:intergenic 20.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5_4:21061458-21061477 None:intergenic 20.0%
!! AATTTGAGTATGATTAAACT+CGG + chr5_4:21062421-21062440 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5_4:21062443-21062462 Msa0843400:intron 20.0%
!! TCTCTCAATTATACTATTTA+GGG + chr5_4:21062481-21062500 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTT+AGG + chr5_4:21062482-21062501 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG + chr5_4:21062685-21062704 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG - chr5_4:21062687-21062706 Msa0843400:CDS 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5_4:21062813-21062832 None:intergenic 20.0%
! TGTTGAGACAAAAAATCAAT+GGG - chr5_4:21061287-21061306 Msa0843400:three_prime_UTR 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5_4:21061505-21061524 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5_4:21061512-21061531 None:intergenic 25.0%
! TGATGAAGTATAAACAAAGA+AGG + chr5_4:21061513-21061532 None:intergenic 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5_4:21061756-21061775 Msa0843400:CDS 25.0%
! AAATCATGTGACTATTCAAA+TGG - chr5_4:21061847-21061866 Msa0843400:CDS 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5_4:21061964-21061983 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5_4:21061962-21061981 Msa0843400:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5_4:21062051-21062070 Msa0843400:CDS 25.0%
!! ACAAACATATAGCTTTTGTT+GGG - chr5_4:21062052-21062071 Msa0843400:CDS 25.0%
! ATTTGAGTATGATTAAACTC+GGG + chr5_4:21062420-21062439 None:intergenic 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5_4:21062606-21062625 None:intergenic 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG - chr5_4:21062701-21062720 Msa0843400:CDS 25.0%
! TACAAAATGATTGTGTTCAT+TGG - chr5_4:21062820-21062839 Msa0843400:five_prime_UTR 25.0%
! TGTTGAGACAAAAAATCAAT+GGG - chr5_4:21061287-21061306 Msa0843400:three_prime_UTR 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5_4:21061505-21061524 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5_4:21061512-21061531 None:intergenic 25.0%
! TGATGAAGTATAAACAAAGA+AGG + chr5_4:21061513-21061532 None:intergenic 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5_4:21061756-21061775 Msa0843400:CDS 25.0%
! AAATCATGTGACTATTCAAA+TGG - chr5_4:21061847-21061866 Msa0843400:CDS 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5_4:21061964-21061983 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5_4:21061962-21061981 Msa0843400:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5_4:21062051-21062070 Msa0843400:CDS 25.0%
!! ACAAACATATAGCTTTTGTT+GGG - chr5_4:21062052-21062071 Msa0843400:CDS 25.0%
! ATTTGAGTATGATTAAACTC+GGG + chr5_4:21062420-21062439 None:intergenic 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5_4:21062606-21062625 None:intergenic 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG - chr5_4:21062701-21062720 Msa0843400:CDS 25.0%
! TACAAAATGATTGTGTTCAT+TGG - chr5_4:21062820-21062839 Msa0843400:five_prime_UTR 25.0%
GTGTTGAGACAAAAAATCAA+TGG - chr5_4:21061286-21061305 Msa0843400:three_prime_UTR 30.0%
CATGAAAGAAAGCTTAGAAA+GGG - chr5_4:21061391-21061410 Msa0843400:CDS 30.0%
CTTAGAAAGGGTTTGAAAAT+GGG - chr5_4:21061403-21061422 Msa0843400:CDS 30.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5_4:21061597-21061616 None:intergenic 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5_4:21061625-21061644 None:intergenic 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5_4:21061765-21061784 Msa0843400:CDS 30.0%
TGACTATTCAAATGGAGATT+GGG - chr5_4:21061855-21061874 Msa0843400:CDS 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5_4:21061897-21061916 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5_4:21061968-21061987 Msa0843400:CDS 30.0%
GTTTGTTCTTAATGAGATTG+AGG + chr5_4:21062038-21062057 None:intergenic 30.0%
! CAAACATATAGCTTTTGTTG+GGG - chr5_4:21062053-21062072 Msa0843400:CDS 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5_4:21062160-21062179 Msa0843400:CDS 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG - chr5_4:21062199-21062218 Msa0843400:CDS 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5_4:21062241-21062260 Msa0843400:CDS 30.0%
GACATGATTGTTATATCAAC+CGG - chr5_4:21062330-21062349 Msa0843400:CDS 30.0%
ACATGATTGTTATATCAACC+GGG - chr5_4:21062331-21062350 Msa0843400:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5_4:21062372-21062391 None:intergenic 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG - chr5_4:21062381-21062400 Msa0843400:CDS 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5_4:21062492-21062511 Msa0843400:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5_4:21062498-21062517 Msa0843400:intron 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5_4:21062609-21062628 Msa0843400:CDS 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5_4:21062777-21062796 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5_4:21062783-21062802 Msa0843400:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5_4:21062858-21062877 None:intergenic 30.0%
TTGTAGAGATGATGAAGAAA+TGG - chr5_4:21062880-21062899 Msa0843400:five_prime_UTR 30.0%
TGTAGAGATGATGAAGAAAT+GGG - chr5_4:21062881-21062900 Msa0843400:five_prime_UTR 30.0%
AGTAACATCAACATAGAGAA+GGG - chr5_4:21062904-21062923 Msa0843400:five_prime_UTR 30.0%
GTGTTGAGACAAAAAATCAA+TGG - chr5_4:21061286-21061305 Msa0843400:three_prime_UTR 30.0%
CATGAAAGAAAGCTTAGAAA+GGG - chr5_4:21061391-21061410 Msa0843400:CDS 30.0%
CTTAGAAAGGGTTTGAAAAT+GGG - chr5_4:21061403-21061422 Msa0843400:CDS 30.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5_4:21061597-21061616 None:intergenic 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5_4:21061625-21061644 None:intergenic 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5_4:21061765-21061784 Msa0843400:CDS 30.0%
TGACTATTCAAATGGAGATT+GGG - chr5_4:21061855-21061874 Msa0843400:CDS 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5_4:21061897-21061916 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5_4:21061968-21061987 Msa0843400:CDS 30.0%
GTTTGTTCTTAATGAGATTG+AGG + chr5_4:21062038-21062057 None:intergenic 30.0%
! CAAACATATAGCTTTTGTTG+GGG - chr5_4:21062053-21062072 Msa0843400:CDS 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5_4:21062160-21062179 Msa0843400:CDS 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG - chr5_4:21062199-21062218 Msa0843400:CDS 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5_4:21062241-21062260 Msa0843400:CDS 30.0%
GACATGATTGTTATATCAAC+CGG - chr5_4:21062330-21062349 Msa0843400:CDS 30.0%
ACATGATTGTTATATCAACC+GGG - chr5_4:21062331-21062350 Msa0843400:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5_4:21062372-21062391 None:intergenic 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG - chr5_4:21062381-21062400 Msa0843400:CDS 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5_4:21062492-21062511 Msa0843400:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5_4:21062498-21062517 Msa0843400:intron 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5_4:21062609-21062628 Msa0843400:CDS 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5_4:21062777-21062796 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5_4:21062783-21062802 Msa0843400:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5_4:21062858-21062877 None:intergenic 30.0%
TTGTAGAGATGATGAAGAAA+TGG - chr5_4:21062880-21062899 Msa0843400:five_prime_UTR 30.0%
TGTAGAGATGATGAAGAAAT+GGG - chr5_4:21062881-21062900 Msa0843400:five_prime_UTR 30.0%
AGTAACATCAACATAGAGAA+GGG - chr5_4:21062904-21062923 Msa0843400:five_prime_UTR 30.0%
CTTTATGAGAGAGTCATGAA+AGG + chr5_4:21061326-21061345 None:intergenic 35.0%
!!! TCACTGTTTTATGGTGTTGT+CGG + chr5_4:21061364-21061383 None:intergenic 35.0%
CACCATAAAACAGTGATTAG+AGG - chr5_4:21061368-21061387 Msa0843400:CDS 35.0%
! TGCCTCTAATCACTGTTTTA+TGG + chr5_4:21061373-21061392 None:intergenic 35.0%
GCATGAAAGAAAGCTTAGAA+AGG - chr5_4:21061390-21061409 Msa0843400:CDS 35.0%
GCTTAGAAAGGGTTTGAAAA+TGG - chr5_4:21061402-21061421 Msa0843400:CDS 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5_4:21061438-21061457 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5_4:21061439-21061458 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5_4:21061593-21061612 None:intergenic 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5_4:21061810-21061829 Msa0843400:CDS 35.0%
GTGACTATTCAAATGGAGAT+TGG - chr5_4:21061854-21061873 Msa0843400:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5_4:21061898-21061917 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG - chr5_4:21061898-21061917 Msa0843400:CDS 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5_4:21061949-21061968 Msa0843400:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5_4:21062095-21062114 None:intergenic 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5_4:21062152-21062171 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5_4:21062166-21062185 None:intergenic 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5_4:21062240-21062259 Msa0843400:CDS 35.0%
! TTTTGGATCTTGTCAATGAG+AGG - chr5_4:21062283-21062302 Msa0843400:CDS 35.0%
GTCCATTTGATCAATATCCT+TGG + chr5_4:21062313-21062332 None:intergenic 35.0%
TTCCATTAAGAAAGGCTTTG+AGG - chr5_4:21062451-21062470 Msa0843400:intron 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5_4:21062543-21062562 Msa0843400:intron 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5_4:21062551-21062570 Msa0843400:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5_4:21062557-21062576 Msa0843400:intron 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5_4:21062596-21062615 Msa0843400:CDS 35.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5_4:21062612-21062631 Msa0843400:CDS 35.0%
AAGGATTGAGATTGAAGAAG+TGG - chr5_4:21062653-21062672 Msa0843400:CDS 35.0%
AGGTATTAGAATTGAGGTGT+TGG - chr5_4:21062707-21062726 Msa0843400:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG - chr5_4:21062722-21062741 Msa0843400:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5_4:21062789-21062808 None:intergenic 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5_4:21062795-21062814 None:intergenic 35.0%
GAGTAACATCAACATAGAGA+AGG - chr5_4:21062903-21062922 Msa0843400:five_prime_UTR 35.0%
CTTTATGAGAGAGTCATGAA+AGG + chr5_4:21061326-21061345 None:intergenic 35.0%
!!! TCACTGTTTTATGGTGTTGT+CGG + chr5_4:21061364-21061383 None:intergenic 35.0%
CACCATAAAACAGTGATTAG+AGG - chr5_4:21061368-21061387 Msa0843400:CDS 35.0%
! TGCCTCTAATCACTGTTTTA+TGG + chr5_4:21061373-21061392 None:intergenic 35.0%
GCATGAAAGAAAGCTTAGAA+AGG - chr5_4:21061390-21061409 Msa0843400:CDS 35.0%
GCTTAGAAAGGGTTTGAAAA+TGG - chr5_4:21061402-21061421 Msa0843400:CDS 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5_4:21061438-21061457 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5_4:21061439-21061458 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5_4:21061593-21061612 None:intergenic 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5_4:21061810-21061829 Msa0843400:CDS 35.0%
GTGACTATTCAAATGGAGAT+TGG - chr5_4:21061854-21061873 Msa0843400:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5_4:21061898-21061917 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG - chr5_4:21061898-21061917 Msa0843400:CDS 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5_4:21061949-21061968 Msa0843400:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5_4:21062095-21062114 None:intergenic 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5_4:21062152-21062171 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5_4:21062166-21062185 None:intergenic 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5_4:21062240-21062259 Msa0843400:CDS 35.0%
! TTTTGGATCTTGTCAATGAG+AGG - chr5_4:21062283-21062302 Msa0843400:CDS 35.0%
GTCCATTTGATCAATATCCT+TGG + chr5_4:21062313-21062332 None:intergenic 35.0%
TTCCATTAAGAAAGGCTTTG+AGG - chr5_4:21062451-21062470 Msa0843400:intron 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5_4:21062543-21062562 Msa0843400:intron 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5_4:21062551-21062570 Msa0843400:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5_4:21062557-21062576 Msa0843400:intron 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5_4:21062596-21062615 Msa0843400:CDS 35.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5_4:21062612-21062631 Msa0843400:CDS 35.0%
AAGGATTGAGATTGAAGAAG+TGG - chr5_4:21062653-21062672 Msa0843400:CDS 35.0%
AGGTATTAGAATTGAGGTGT+TGG - chr5_4:21062707-21062726 Msa0843400:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG - chr5_4:21062722-21062741 Msa0843400:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5_4:21062789-21062808 None:intergenic 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5_4:21062795-21062814 None:intergenic 35.0%
GAGTAACATCAACATAGAGA+AGG - chr5_4:21062903-21062922 Msa0843400:five_prime_UTR 35.0%
GAGTCATGAAAGGAAGAGAA+AGG + chr5_4:21061316-21061335 None:intergenic 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5_4:21061440-21061459 None:intergenic 40.0%
! AGTTGGTGCAAAAGGAGTAA+GGG + chr5_4:21061542-21061561 None:intergenic 40.0%
! TAGTTGGTGCAAAAGGAGTA+AGG + chr5_4:21061543-21061562 None:intergenic 40.0%
GTAGAATGAAGCAGCTTAGT+TGG + chr5_4:21061559-21061578 None:intergenic 40.0%
TGAATAGTGGTAGGTGGAAA+TGG + chr5_4:21061584-21061603 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5_4:21061590-21061609 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5_4:21061622-21061641 None:intergenic 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5_4:21061619-21061638 Msa0843400:CDS 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5_4:21061914-21061933 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5_4:21061934-21061953 Msa0843400:CDS 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5_4:21061997-21062016 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG - chr5_4:21061995-21062014 Msa0843400:CDS 40.0%
! AAAGTGCTTGGTTCAAACCT+TGG + chr5_4:21062015-21062034 None:intergenic 40.0%
!! ATGAGATTGAGGAAAGTGCT+TGG + chr5_4:21062027-21062046 None:intergenic 40.0%
! AGCTTTTGTTGGGGATTCTT+TGG - chr5_4:21062062-21062081 Msa0843400:CDS 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5_4:21062138-21062157 None:intergenic 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5_4:21062135-21062154 Msa0843400:CDS 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5_4:21062143-21062162 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG + chr5_4:21062208-21062227 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5_4:21062226-21062245 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5_4:21062227-21062246 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5_4:21062228-21062247 None:intergenic 40.0%
GGCCAAGGATATTGATCAAA+TGG - chr5_4:21062308-21062327 Msa0843400:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5_4:21062337-21062356 Msa0843400:CDS 40.0%
GTCCTCAAAGCCTTTCTTAA+TGG + chr5_4:21062456-21062475 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5_4:21062552-21062571 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5_4:21062550-21062569 Msa0843400:intron 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5_4:21062617-21062636 Msa0843400:CDS 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5_4:21062621-21062640 Msa0843400:CDS 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5_4:21062634-21062653 Msa0843400:CDS 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5_4:21062741-21062760 Msa0843400:CDS 40.0%
AAAGGATTCATGTAAGGACC+TGG + chr5_4:21062771-21062790 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5_4:21062852-21062871 None:intergenic 40.0%
GAGTCATGAAAGGAAGAGAA+AGG + chr5_4:21061316-21061335 None:intergenic 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5_4:21061440-21061459 None:intergenic 40.0%
! AGTTGGTGCAAAAGGAGTAA+GGG + chr5_4:21061542-21061561 None:intergenic 40.0%
! TAGTTGGTGCAAAAGGAGTA+AGG + chr5_4:21061543-21061562 None:intergenic 40.0%
GTAGAATGAAGCAGCTTAGT+TGG + chr5_4:21061559-21061578 None:intergenic 40.0%
TGAATAGTGGTAGGTGGAAA+TGG + chr5_4:21061584-21061603 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5_4:21061590-21061609 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5_4:21061622-21061641 None:intergenic 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5_4:21061619-21061638 Msa0843400:CDS 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5_4:21061914-21061933 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5_4:21061934-21061953 Msa0843400:CDS 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5_4:21061997-21062016 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG - chr5_4:21061995-21062014 Msa0843400:CDS 40.0%
! AAAGTGCTTGGTTCAAACCT+TGG + chr5_4:21062015-21062034 None:intergenic 40.0%
!! ATGAGATTGAGGAAAGTGCT+TGG + chr5_4:21062027-21062046 None:intergenic 40.0%
! AGCTTTTGTTGGGGATTCTT+TGG - chr5_4:21062062-21062081 Msa0843400:CDS 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5_4:21062138-21062157 None:intergenic 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5_4:21062135-21062154 Msa0843400:CDS 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5_4:21062143-21062162 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG + chr5_4:21062208-21062227 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5_4:21062226-21062245 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5_4:21062227-21062246 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5_4:21062228-21062247 None:intergenic 40.0%
GGCCAAGGATATTGATCAAA+TGG - chr5_4:21062308-21062327 Msa0843400:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5_4:21062337-21062356 Msa0843400:CDS 40.0%
GTCCTCAAAGCCTTTCTTAA+TGG + chr5_4:21062456-21062475 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5_4:21062552-21062571 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5_4:21062550-21062569 Msa0843400:intron 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5_4:21062617-21062636 Msa0843400:CDS 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5_4:21062621-21062640 Msa0843400:CDS 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5_4:21062634-21062653 Msa0843400:CDS 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5_4:21062741-21062760 Msa0843400:CDS 40.0%
AAAGGATTCATGTAAGGACC+TGG + chr5_4:21062771-21062790 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5_4:21062852-21062871 None:intergenic 40.0%
! AGCAGCTTAGTTGGTGCAAA+AGG + chr5_4:21061550-21061569 None:intergenic 45.0%
ATAGTGGTAGGTGGAAATGG+TGG + chr5_4:21061581-21061600 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5_4:21061871-21061890 Msa0843400:CDS 45.0%
TTGTTGGGGATTCTTTGGCT+AGG - chr5_4:21062067-21062086 Msa0843400:CDS 45.0%
TGGATCTTGTCAATGAGAGG+TGG - chr5_4:21062286-21062305 Msa0843400:CDS 45.0%
GGATCTTGTCAATGAGAGGT+GGG - chr5_4:21062287-21062306 Msa0843400:CDS 45.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5_4:21062352-21062371 None:intergenic 45.0%
! TGTGTTCATTGGTGTTTGCC+AGG - chr5_4:21062831-21062850 Msa0843400:five_prime_UTR 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5_4:21062847-21062866 Msa0843400:five_prime_UTR 45.0%
! AGCAGCTTAGTTGGTGCAAA+AGG + chr5_4:21061550-21061569 None:intergenic 45.0%
ATAGTGGTAGGTGGAAATGG+TGG + chr5_4:21061581-21061600 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5_4:21061871-21061890 Msa0843400:CDS 45.0%
TTGTTGGGGATTCTTTGGCT+AGG - chr5_4:21062067-21062086 Msa0843400:CDS 45.0%
TGGATCTTGTCAATGAGAGG+TGG - chr5_4:21062286-21062305 Msa0843400:CDS 45.0%
GGATCTTGTCAATGAGAGGT+GGG - chr5_4:21062287-21062306 Msa0843400:CDS 45.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5_4:21062352-21062371 None:intergenic 45.0%
! TGTGTTCATTGGTGTTTGCC+AGG - chr5_4:21062831-21062850 Msa0843400:five_prime_UTR 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5_4:21062847-21062866 Msa0843400:five_prime_UTR 45.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5_4:21062219-21062238 Msa0843400:CDS 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5_4:21062561-21062580 Msa0843400:intron 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5_4:21062750-21062769 Msa0843400:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5_4:21062759-21062778 None:intergenic 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5_4:21062219-21062238 Msa0843400:CDS 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5_4:21062561-21062580 Msa0843400:intron 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5_4:21062750-21062769 Msa0843400:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5_4:21062759-21062778 None:intergenic 50.0%
TGTCAATGAGAGGTGGGCCA+AGG - chr5_4:21062293-21062312 Msa0843400:CDS 55.0%
TGTCAATGAGAGGTGGGCCA+AGG - chr5_4:21062293-21062312 Msa0843400:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 21061282 21062953 21061282 ID=Msa0843400;Name=Msa0843400
chr5_4 mRNA 21061282 21062953 21061282 ID=Msa0843400-mRNA-1;Parent=Msa0843400;Name=Msa0843400-mRNA-1;_AED=0.05;_eAED=0.05;_QI=139|1|1|1|1|1|2|45|435
chr5_4 exon 21061282 21062414 21061282 ID=Msa0843400-mRNA-1:exon:8718;Parent=Msa0843400-mRNA-1
chr5_4 exon 21062595 21062953 21062595 ID=Msa0843400-mRNA-1:exon:8717;Parent=Msa0843400-mRNA-1
chr5_4 five_prime_UTR 21062815 21062953 21062815 ID=Msa0843400-mRNA-1:five_prime_utr;Parent=Msa0843400-mRNA-1
chr5_4 CDS 21062595 21062814 21062595 ID=Msa0843400-mRNA-1:cds;Parent=Msa0843400-mRNA-1
chr5_4 CDS 21061327 21062414 21061327 ID=Msa0843400-mRNA-1:cds;Parent=Msa0843400-mRNA-1
chr5_4 three_prime_UTR 21061282 21061326 21061282 ID=Msa0843400-mRNA-1:three_prime_utr;Parent=Msa0843400-mRNA-1
Gene Sequence

>Msa0843400

ATGGGAAGCACAAACCCCTTCAATGACAATCTTTCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCATTTTGTCCCTTACTCCTTTTGCACCAACTAAGCTGCTTCATTCTACCACCATTTCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGACTATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAAAGTGAGAGATGCATCACAAATGGTAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGGTTTGAACCAAGCACTTTCCTCAATCTCATTAAGAACAAACATATAGCTTTTGTTGGGGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTATTGACTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCTTTCTCATAATGCTACCTTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAGGTGGGCCAAGGATATTGATCAAATGGACATGATTGTTATATCAACCGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAGTTCTTGGTAGCTTGAATTGTCCCGAGTTTAATCATACTCAAATTGATTTTTATGTTCCATTAAGAAAGGCTTTGAGGACTACCCTAAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGATAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGAGATTGAAGAAGTGGAAAGTGCCAAGACAAAAGCTAATAAATTTGAAGGTATTAGAATTGAGGTGTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAATGATTGTGTTCATTGGTGTTTGCCAGGACCTATAGATACTTGGAATGAGATTTTTGTAGAGATGATGAAGAAATGGGAGTAA
Protein sequence

>Msa0843400

MGSTNPFNDNLSSFNKKFFHLTLYSLLPISLLCLYFIILSLTPFAPTKLLHSTTISTYHYSSTYSSPPSTSFVGKNKVYDYEKSCDYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPSTFLNLIKNKHIAFVGDSLARNQLESLLCMLLTTSTPNLVYQDGKSNKFRKWHFLSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNERWAKDIDQMDMIVISTGHWFLLPSIYYESDLVLGSLNCPEFNHTQIDFYVPLRKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEDKIVGGMDGEIRRIEIEEVESAKTKANKFEGIRIEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFVEMMKKWE*