AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0732080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732080 MtrunA17_Chr5g0426551 99.615 260 1 0 1 260 1 260 0.0 532
Msa0732080 MtrunA17_Chr3g0132301 90.385 260 25 0 1 260 1 260 9.36e-177 486
Msa0732080 MtrunA17_Chr3g0133161 75.949 237 56 1 8 243 11 247 1.98e-135 382
Msa0732080 MtrunA17_Chr2g0325591 72.016 243 66 1 8 250 7 247 7.25e-129 365
Msa0732080 MtrunA17_Chr2g0283791 69.020 255 72 3 6 256 3 254 4.36e-126 358
Msa0732080 MtrunA17_Chr8g0379011 71.967 239 65 1 8 246 7 243 1.50e-125 356
Msa0732080 MtrunA17_Chr5g0418971 70.325 246 71 1 3 248 2 245 1.71e-124 354
Msa0732080 MtrunA17_Chr2g0316011 66.154 260 85 2 1 259 1 258 8.43e-124 353
Msa0732080 MtrunA17_Chr4g0043141 64.017 239 65 2 27 246 1 237 1.15e-105 306
Msa0732080 MtrunA17_Chr1g0164601 58.723 235 91 4 8 240 11 241 7.81e-91 269
Msa0732080 MtrunA17_Chr3g0082111 58.298 235 92 4 8 240 41 271 5.11e-90 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732080 AT1G78300.1 90.157 254 24 1 8 260 5 258 1.78e-169 468
Msa0732080 AT1G35160.1 85.985 264 33 2 1 260 1 264 1.87e-167 464
Msa0732080 AT4G09000.1 88.933 253 26 1 1 251 1 253 2.54e-166 461
Msa0732080 AT1G35160.2 77.740 292 33 3 1 260 1 292 3.52e-161 449
Msa0732080 AT4G09000.2 89.344 244 24 1 1 242 1 244 2.71e-160 447
Msa0732080 AT5G38480.1 81.496 254 46 1 6 258 2 255 3.30e-154 429
Msa0732080 AT3G02520.1 80.784 255 48 1 6 259 3 257 1.34e-152 426
Msa0732080 AT3G02520.2 80.784 255 48 1 6 259 3 257 1.34e-152 426
Msa0732080 AT5G38480.3 81.496 254 45 2 6 258 2 254 1.02e-151 423
Msa0732080 AT5G38480.2 81.496 254 45 2 6 258 2 254 1.02e-151 423
Msa0732080 AT5G10450.2 79.508 244 49 1 4 246 3 246 3.72e-145 406
Msa0732080 AT5G10450.1 79.668 241 48 1 4 243 3 243 8.60e-145 405
Msa0732080 AT5G10450.4 77.510 249 55 1 4 251 3 251 8.93e-145 406
Msa0732080 AT5G10450.3 80.000 240 47 1 4 242 3 242 5.64e-144 404
Msa0732080 AT5G16050.1 79.310 261 50 2 3 260 2 261 3.35e-143 402
Msa0732080 AT5G16050.2 79.310 261 50 2 3 260 2 261 3.35e-143 402
Msa0732080 AT5G65430.2 78.138 247 52 2 1 246 1 246 2.19e-142 399
Msa0732080 AT5G65430.1 78.279 244 51 2 1 243 1 243 6.17e-142 398
Msa0732080 AT5G65430.3 78.601 243 50 2 1 242 1 242 2.83e-141 397
Msa0732080 AT1G26480.1 74.180 244 60 3 8 250 10 251 7.56e-129 366
Msa0732080 AT2G42590.3 70.539 241 69 1 8 248 7 245 3.36e-122 349
Msa0732080 AT2G42590.1 70.539 241 69 1 8 248 7 245 3.39e-122 349
Msa0732080 AT2G42590.2 70.833 240 68 1 8 247 7 244 9.15e-122 348
Msa0732080 AT1G34760.2 71.008 238 67 1 8 245 5 240 3.80e-121 346
Msa0732080 AT1G34760.1 71.008 238 67 1 8 245 5 240 4.76e-121 346
Msa0732080 AT1G22300.2 68.254 252 73 2 8 259 5 249 6.77e-117 335
Msa0732080 AT1G22300.1 68.254 252 73 2 8 259 5 249 6.77e-117 335
Msa0732080 AT1G22300.3 69.874 239 70 1 8 246 5 241 1.40e-115 332
Msa0732080 AT1G78220.1 48.927 233 116 2 8 239 5 235 1.31e-73 225
Msa0732080 AT1G22290.2 45.933 209 91 6 12 217 9 198 4.64e-48 159
Msa0732080 AT1G22290.1 45.631 206 90 6 12 214 9 195 6.36e-48 157
Msa0732080 AT2G10450.1 71.429 49 14 0 207 255 16 64 7.81e-18 76.3

Find 77 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCAAATCCGTACAAATT+TGG 0.140829 5_1:+43980472 Msa0732080:five_prime_UTR
AAAATTGTAAAAGATGAATT+TGG 0.144512 5_1:+43980404 Msa0732080:five_prime_UTR
TGCTGCTTCTGGTGATTCTA+AGG 0.228252 5_1:+43981028 Msa0732080:CDS
AAGAATACCGTTACAGATAT+TGG 0.234430 5_1:-43980976 None:intergenic
TGCCAAATCCGTACAAATTT+GGG 0.243717 5_1:+43980473 Msa0732080:five_prime_UTR
TACTGCTCATCGGCGCCTTT+TGG 0.244944 5_1:-43982664 None:intergenic
TTGATTCCTTCTGCTGCTTC+TGG 0.276388 5_1:+43981017 Msa0732080:CDS
CGATGAGGCTATTGCTGAAT+TGG 0.295305 5_1:+43982423 Msa0732080:CDS
AATGGAAGAACAAACTAAAA+TGG 0.303214 5_1:-43982830 None:intergenic
CGAAGAGATGGTCGATTTCA+TGG 0.314624 5_1:+43980749 Msa0732080:CDS
AAATTTCTATCATATGAAAT+TGG 0.325146 5_1:+43982895 Msa0732080:three_prime_UTR
TGCTTGTGGCCTTGCAAAAC+AGG 0.353700 5_1:+43981956 Msa0732080:CDS
GATAATCACCTTTCATCTTA+AGG 0.367061 5_1:-43981058 None:intergenic
AAATTGTAAAAGATGAATTT+GGG 0.395537 5_1:+43980405 Msa0732080:five_prime_UTR
TTGTTTGGGTTTCAGGATGA+TGG 0.406709 5_1:+43982616 Msa0732080:intron
TTCGTAACGTTCGGCTTGTT+CGG 0.410967 5_1:-43980730 None:intergenic
TTTATGATATATGGATGCAC+TGG 0.414849 5_1:+43982806 Msa0732080:three_prime_UTR
AGAGCAAGACCAAGCCTGAT+TGG 0.420669 5_1:-43981888 None:intergenic
TCCGTGATAACCTCACCCTT+TGG 0.424479 5_1:+43982497 Msa0732080:CDS
TGAATTTGGGTCTTCACTCT+TGG 0.434577 5_1:+43980418 Msa0732080:five_prime_UTR
GACCATCTCTTCGTAACGTT+CGG 0.437327 5_1:-43980739 None:intergenic
AGATGAAAGGTGATTATCAT+AGG 0.447012 5_1:+43981063 Msa0732080:CDS
TACCACCAACTCACCCAATC+AGG 0.450085 5_1:+43981874 Msa0732080:CDS
TTCAGAAACACGTACACAAA+TGG 0.452182 5_1:-43982848 None:intergenic
GCAAGGCCACAAGCACGATC+AGG 0.466255 5_1:-43981948 None:intergenic
ATTGCTGAATTGGATACATT+GGG 0.469687 5_1:+43982433 Msa0732080:CDS
GTTGGTGCGGTTGCCATGGA+TGG 0.469829 5_1:-43980672 None:intergenic
GCTTTCTTCCTTCTGTTCGA+TGG 0.473046 5_1:-43980889 None:intergenic
TACTGCTTATAAAGCTGCTC+AGG 0.475422 5_1:+43981142 Msa0732080:CDS
ATCAAAGAAGCCGCACCAAA+AGG 0.476807 5_1:+43982649 Msa0732080:CDS
GAGCAAGACCAAGCCTGATT+GGG 0.484933 5_1:-43981887 None:intergenic
AAACTCTTCACTCTTCAGAC+AGG 0.486125 5_1:+43983032 Msa0732080:three_prime_UTR
TTGTGAGAAATGTTTGTAAA+GGG 0.487958 5_1:-43980587 None:intergenic
TATTGCTGAATTGGATACAT+TGG 0.488219 5_1:+43982432 Msa0732080:CDS
ACGTGAAGAGTTCGTCTACA+TGG 0.496790 5_1:+43980698 Msa0732080:CDS
TGGTGTTGGTGCGGTTGCCA+TGG 0.499485 5_1:-43980676 None:intergenic
GGTGAGTTCTTCGCTTTCGA+CGG 0.507397 5_1:-43980787 None:intergenic
TCGGAGCAAGACGTGCTTCG+TGG 0.514368 5_1:+43980853 Msa0732080:CDS
TTGCCATGGATGGATGAATC+TGG 0.516725 5_1:-43980662 None:intergenic
TTTGTGAGAAATGTTTGTAA+AGG 0.520519 5_1:-43980588 None:intergenic
AACTCTCCTGATCGTGCTTG+TGG 0.523777 5_1:+43981942 Msa0732080:CDS
CCTTTGGACCTCTGACATGC+AGG 0.533382 5_1:+43982513 Msa0732080:CDS
TGAGAAATGTTTGTAAAGGG+TGG 0.536454 5_1:-43980584 None:intergenic
TTCTTCGCTTTCGACGGCGG+CGG 0.538091 5_1:-43980781 None:intergenic
CCAACTCACCCAATCAGGCT+TGG 0.548958 5_1:+43981879 Msa0732080:CDS
CATCTCTTCCATCGAACAGA+AGG 0.549390 5_1:+43980881 Msa0732080:CDS
CCTGCATGTCAGAGGTCCAA+AGG 0.556945 5_1:-43982513 None:intergenic
CTGCATGTCAGAGGTCCAAA+GGG 0.558806 5_1:-43982512 None:intergenic
AGCCTGATTGGGTGAGTTGG+TGG 0.564969 5_1:-43981876 None:intergenic
TGAATTGGATACATTGGGAG+AGG 0.566946 5_1:+43982438 Msa0732080:CDS
CTCTTCACGTGGTGTTGGTG+CGG 0.568646 5_1:-43980685 None:intergenic
AACTCTTCACTCTTCAGACA+GGG 0.571026 5_1:+43983033 Msa0732080:three_prime_UTR
GGATGGATGAATCTGGTTGA+AGG 0.574115 5_1:-43980655 None:intergenic
TCCAAAGGGTGAGGTTATCA+CGG 0.578031 5_1:-43982498 None:intergenic
ACGAACTCTTCACGTGGTGT+TGG 0.578137 5_1:-43980690 None:intergenic
CCAAGCCTGATTGGGTGAGT+TGG 0.579199 5_1:-43981879 None:intergenic
CAACCAGATTCATCCATCCA+TGG 0.582939 5_1:+43980659 Msa0732080:exon
GAGTTCTTCGCTTTCGACGG+CGG 0.587801 5_1:-43980784 None:intergenic
GAACTTTCCAATATCTGTAA+CGG 0.597556 5_1:+43980969 Msa0732080:CDS
GCCAAATCCGTACAAATTTG+GGG 0.605960 5_1:+43980474 Msa0732080:five_prime_UTR
TGTCAGAGGTCCAAAGGGTG+AGG 0.606809 5_1:-43982507 None:intergenic
TTATGATATATGGATGCACT+GGG 0.612064 5_1:+43982807 Msa0732080:three_prime_UTR
AGATCTGTAATCACGAATCA+CGG 0.615306 5_1:-43980934 None:intergenic
GAATCACCAGAAGCAGCAGA+AGG 0.617542 5_1:-43981023 None:intergenic
TATCTTGCTGAGTTTAAGAG+TGG 0.621733 5_1:+43981086 Msa0732080:CDS
AGCCGAACGTTACGAAGAGA+TGG 0.627906 5_1:+43980737 Msa0732080:CDS
ACGATATGATTACTGCTCAT+CGG 0.628913 5_1:-43982674 None:intergenic
ATATCATACCTGCATGTCAG+AGG 0.643750 5_1:-43982521 None:intergenic
ATTGGGAGAGGAATCATACA+AGG 0.653137 5_1:+43982450 Msa0732080:CDS
GAGGAGGTTACGTTCTTCGA+CGG 0.656962 5_1:-43980808 None:intergenic
AAAGGGTGGATGTGAATGAG+AGG 0.658640 5_1:-43980570 None:intergenic
TAACATGTTCATCGTTACCA+CGG 0.672519 5_1:-43980911 None:intergenic
CCTTTCAAAACATGATACAG+AGG 0.674464 5_1:-43982724 None:intergenic
GAACAGAAGGAAGAAAGCCG+TGG 0.676297 5_1:+43980894 Msa0732080:CDS
CCAAATCCGTACAAATTTGG+GGG 0.676426 5_1:+43980475 Msa0732080:five_prime_UTR
TATTCATTAGGCTTTCGATG+AGG 0.689894 5_1:+43982408 Msa0732080:intron
ATGTAGACGAACTCTTCACG+TGG 0.738511 5_1:-43980696 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTGTAAAAGATGAATT+TGG + chr5_1:43980404-43980423 Msa0732080:five_prime_UTR 15.0%
!! AAATTGTAAAAGATGAATTT+GGG + chr5_1:43980405-43980424 Msa0732080:five_prime_UTR 15.0%
!!! GAAATTTATTGCAATTTTTA+TGG + chr5_1:43981369-43981388 Msa0732080:intron 15.0%
!!! TGTTTATTTTTGGTTTAAAA+TGG + chr5_1:43981582-43981601 Msa0732080:intron 15.0%
!! TTAATTAAACATAATCATGT+CGG - chr5_1:43981733-43981752 None:intergenic 15.0%
!!! ATTTTGGTTTATATTTTGTT+TGG + chr5_1:43982601-43982620 Msa0732080:intron 15.0%
!!! TTTTGGTTTATATTTTGTTT+GGG + chr5_1:43982602-43982621 Msa0732080:intron 15.0%
!! AAATTTCTATCATATGAAAT+TGG + chr5_1:43982895-43982914 Msa0732080:three_prime_UTR 15.0%
!!! TCATCTTTTACAATTTTCTT+AGG - chr5_1:43980401-43980420 None:intergenic 20.0%
!! GCAATATTTCAAACAAATAA+TGG - chr5_1:43981324-43981343 None:intergenic 20.0%
!! TCTCAATTAGGTTAAAATAA+TGG + chr5_1:43981511-43981530 Msa0732080:intron 20.0%
!!! TTTTGAAATGAGTTGAAAAT+AGG + chr5_1:43981555-43981574 Msa0732080:intron 20.0%
!!! TTTGAAATGAGTTGAAAATA+GGG + chr5_1:43981556-43981575 Msa0732080:intron 20.0%
!!! AATAGGGTATTGTTTATTTT+TGG + chr5_1:43981572-43981591 Msa0732080:intron 20.0%
!! ACTACAAACTTTAATTGAAA+AGG - chr5_1:43982050-43982069 None:intergenic 20.0%
!!! TTTTCAATTAAAGTTTGTAG+TGG + chr5_1:43982049-43982068 Msa0732080:intron 20.0%
!! TTCACATCAACATTAAAAAA+GGG - chr5_1:43982257-43982276 None:intergenic 20.0%
!! ATTCACATCAACATTAAAAA+AGG - chr5_1:43982258-43982277 None:intergenic 20.0%
!! TAATGAATATAGAAAAGAAC+AGG - chr5_1:43982397-43982416 None:intergenic 20.0%
!!! TGTTCTTTTCTATATTCATT+AGG + chr5_1:43982396-43982415 Msa0732080:intron 20.0%
!!! CATATTCTGTTTATTGATTT+TGG + chr5_1:43982585-43982604 Msa0732080:intron 20.0%
!!! ATGGTCTATTTTATGATATA+TGG + chr5_1:43982797-43982816 Msa0732080:three_prime_UTR 20.0%
!!! GGGTTAACTATTTTTAATTC+CGG - chr5_1:43980515-43980534 None:intergenic 25.0%
!!! ATAGCAGTTTTTTGTTTTAG+TGG - chr5_1:43980545-43980564 None:intergenic 25.0%
! TTGTGAGAAATGTTTGTAAA+GGG - chr5_1:43980590-43980609 None:intergenic 25.0%
! TTTGTGAGAAATGTTTGTAA+AGG - chr5_1:43980591-43980610 None:intergenic 25.0%
! GAGATCTAACGTTATATTTA+TGG + chr5_1:43981180-43981199 Msa0732080:intron 25.0%
!! TCTATAACTGACTTTAGTTT+AGG + chr5_1:43981346-43981365 Msa0732080:intron 25.0%
!! CTATAACTGACTTTAGTTTA+GGG + chr5_1:43981347-43981366 Msa0732080:intron 25.0%
!!! TTTTTTTTTGGTGATGTGAT+TGG + chr5_1:43981441-43981460 Msa0732080:intron 25.0%
!! ATTTGACGATTTTCTCAATT+AGG + chr5_1:43981499-43981518 Msa0732080:intron 25.0%
!! AAAATGGAAGTCTTATATGA+TGG + chr5_1:43981598-43981617 Msa0732080:intron 25.0%
!! CATTTTCTCAACTTTTTAAC+TGG + chr5_1:43981756-43981775 Msa0732080:intron 25.0%
! ACTACAGACTTTAATTGAAA+AGG - chr5_1:43982111-43982130 None:intergenic 25.0%
!! TTTTCAATTAAAGTCTGTAG+TGG + chr5_1:43982110-43982129 Msa0732080:intron 25.0%
! TAATTTCATTAACGTGTTTG+TGG + chr5_1:43982210-43982229 Msa0732080:intron 25.0%
! AATTTCATTAACGTGTTTGT+GGG + chr5_1:43982211-43982230 Msa0732080:intron 25.0%
!! AATGTTGATGTGAATTTGTT+AGG + chr5_1:43982263-43982282 Msa0732080:intron 25.0%
! TATTTGTTCCTCAATAAGTT+TGG + chr5_1:43982315-43982334 Msa0732080:intron 25.0%
!! TGATTCTGTTCTGTTATTTA+TGG + chr5_1:43982351-43982370 Msa0732080:intron 25.0%
!!! TTATATTTTGTTTGGGTTTC+AGG + chr5_1:43982609-43982628 Msa0732080:intron 25.0%
! AATGGAAGAACAAACTAAAA+TGG - chr5_1:43982833-43982852 None:intergenic 25.0%
!!! CAGTTTTTTGTTTTAGTGGA+CGG - chr5_1:43980541-43980560 None:intergenic 30.0%
GAACTTTCCAATATCTGTAA+CGG + chr5_1:43980969-43980988 Msa0732080:CDS 30.0%
AAGAATACCGTTACAGATAT+TGG - chr5_1:43980979-43980998 None:intergenic 30.0%
! GTTTTCTACCTTAAGATGAA+AGG + chr5_1:43981050-43981069 Msa0732080:CDS 30.0%
GATAATCACCTTTCATCTTA+AGG - chr5_1:43981061-43981080 None:intergenic 30.0%
AGATGAAAGGTGATTATCAT+AGG + chr5_1:43981063-43981082 Msa0732080:CDS 30.0%
TTAGATCTCAGAGAAAAAGA+TGG - chr5_1:43981169-43981188 None:intergenic 30.0%
!! TTTAATTTGACTCTCCTTGT+AGG + chr5_1:43981833-43981852 Msa0732080:intron 30.0%
! TGTTCTAGATACCGAATAAT+GGG + chr5_1:43981983-43982002 Msa0732080:intron 30.0%
TATTGCTGAATTGGATACAT+TGG + chr5_1:43982432-43982451 Msa0732080:CDS 30.0%
ATTGCTGAATTGGATACATT+GGG + chr5_1:43982433-43982452 Msa0732080:CDS 30.0%
!!! CTCTGTATCATGTTTTGAAA+GGG + chr5_1:43982725-43982744 Msa0732080:three_prime_UTR 30.0%
!!! TCTGTATCATGTTTTGAAAG+GGG + chr5_1:43982726-43982745 Msa0732080:three_prime_UTR 30.0%
TTTATGATATATGGATGCAC+TGG + chr5_1:43982806-43982825 Msa0732080:three_prime_UTR 30.0%
TTATGATATATGGATGCACT+GGG + chr5_1:43982807-43982826 Msa0732080:three_prime_UTR 30.0%
!!! TCAGCTGATTTTTTTCATTG+AGG + chr5_1:43982947-43982966 Msa0732080:three_prime_UTR 30.0%
ATGCCAAATCCGTACAAATT+TGG + chr5_1:43980472-43980491 Msa0732080:five_prime_UTR 35.0%
TGCCAAATCCGTACAAATTT+GGG + chr5_1:43980473-43980492 Msa0732080:five_prime_UTR 35.0%
AATTTGCCCCCAAATTTGTA+CGG - chr5_1:43980484-43980503 None:intergenic 35.0%
TGAGAAATGTTTGTAAAGGG+TGG - chr5_1:43980587-43980606 None:intergenic 35.0%
TAACATGTTCATCGTTACCA+CGG - chr5_1:43980914-43980933 None:intergenic 35.0%
AGATCTGTAATCACGAATCA+CGG - chr5_1:43980937-43980956 None:intergenic 35.0%
TATCTTGCTGAGTTTAAGAG+TGG + chr5_1:43981086-43981105 Msa0732080:CDS 35.0%
CGCAAATCTTCAAATCCAAA+CGG - chr5_1:43981804-43981823 None:intergenic 35.0%
! GTGTTCTAGATACCGAATAA+TGG + chr5_1:43981982-43982001 Msa0732080:intron 35.0%
GAACATCATCTCCCATTATT+CGG - chr5_1:43981997-43982016 None:intergenic 35.0%
AATGGGAGATGATGTTCATT+TGG + chr5_1:43982000-43982019 Msa0732080:intron 35.0%
!! GATGTGAATTTGTTAGGTTC+TGG + chr5_1:43982269-43982288 Msa0732080:intron 35.0%
ATGCTACACCAAACTTATTG+AGG - chr5_1:43982326-43982345 None:intergenic 35.0%
TATTCATTAGGCTTTCGATG+AGG + chr5_1:43982408-43982427 Msa0732080:intron 35.0%
ACGATATGATTACTGCTCAT+CGG - chr5_1:43982677-43982696 None:intergenic 35.0%
CCTTTCAAAACATGATACAG+AGG - chr5_1:43982727-43982746 None:intergenic 35.0%
!! CCTCTGTATCATGTTTTGAA+AGG + chr5_1:43982724-43982743 Msa0732080:three_prime_UTR 35.0%
! GTTGATGCTACAGAGATTTT+AGG + chr5_1:43982769-43982788 Msa0732080:three_prime_UTR 35.0%
! ACAGAGATTTTAGGCATTCA+TGG + chr5_1:43982778-43982797 Msa0732080:three_prime_UTR 35.0%
TTCAGAAACACGTACACAAA+TGG - chr5_1:43982851-43982870 None:intergenic 35.0%
TGAATTTGGGTCTTCACTCT+TGG + chr5_1:43980418-43980437 Msa0732080:five_prime_UTR 40.0%
GCCAAATCCGTACAAATTTG+GGG + chr5_1:43980474-43980493 Msa0732080:five_prime_UTR 40.0%
CCAAATCCGTACAAATTTGG+GGG + chr5_1:43980475-43980494 Msa0732080:five_prime_UTR 40.0%
!!! TTTGTTTTAGTGGACGGTGA+GGG - chr5_1:43980535-43980554 None:intergenic 40.0%
!!! TTTTGTTTTAGTGGACGGTG+AGG - chr5_1:43980536-43980555 None:intergenic 40.0%
TACTGCTTATAAAGCTGCTC+AGG + chr5_1:43981142-43981161 Msa0732080:CDS 40.0%
CACCTTTGATCAAATGTGTC+TGG + chr5_1:43981704-43981723 Msa0732080:intron 40.0%
CACCAGACACATTTGATCAA+AGG - chr5_1:43981709-43981728 None:intergenic 40.0%
! GTGTTATTGTTGTGTCCGTT+TGG + chr5_1:43981786-43981805 Msa0732080:intron 40.0%
AGAGTTAGCAATGTCCTACA+AGG - chr5_1:43981850-43981869 None:intergenic 40.0%
! GAACACTAACCTGTTTTGCA+AGG - chr5_1:43981968-43981987 None:intergenic 40.0%
TGAATTGGATACATTGGGAG+AGG + chr5_1:43982438-43982457 Msa0732080:CDS 40.0%
ATTGGGAGAGGAATCATACA+AGG + chr5_1:43982450-43982469 Msa0732080:CDS 40.0%
ATATCATACCTGCATGTCAG+AGG - chr5_1:43982524-43982543 None:intergenic 40.0%
! TTGTTTGGGTTTCAGGATGA+TGG + chr5_1:43982616-43982635 Msa0732080:intron 40.0%
!!! TCATGTTTTGAAAGGGGAGA+AGG + chr5_1:43982732-43982751 Msa0732080:three_prime_UTR 40.0%
AAACTCTTCACTCTTCAGAC+AGG + chr5_1:43983032-43983051 Msa0732080:three_prime_UTR 40.0%
AACTCTTCACTCTTCAGACA+GGG + chr5_1:43983033-43983052 Msa0732080:three_prime_UTR 40.0%
CCCCCAAATTTGTACGGATT+TGG - chr5_1:43980478-43980497 None:intergenic 45.0%
GGGGGCAAATTAGTGATTTC+CGG + chr5_1:43980493-43980512 Msa0732080:five_prime_UTR 45.0%
AAAGGGTGGATGTGAATGAG+AGG - chr5_1:43980573-43980592 None:intergenic 45.0%
GGATGGATGAATCTGGTTGA+AGG - chr5_1:43980658-43980677 None:intergenic 45.0%
CAACCAGATTCATCCATCCA+TGG + chr5_1:43980659-43980678 Msa0732080:exon 45.0%
TTGCCATGGATGGATGAATC+TGG - chr5_1:43980665-43980684 None:intergenic 45.0%
ATGTAGACGAACTCTTCACG+TGG - chr5_1:43980699-43980718 None:intergenic 45.0%
ACGTGAAGAGTTCGTCTACA+TGG + chr5_1:43980698-43980717 Msa0732080:CDS 45.0%
! TTCGTAACGTTCGGCTTGTT+CGG - chr5_1:43980733-43980752 None:intergenic 45.0%
GACCATCTCTTCGTAACGTT+CGG - chr5_1:43980742-43980761 None:intergenic 45.0%
! CGAAGAGATGGTCGATTTCA+TGG + chr5_1:43980749-43980768 Msa0732080:CDS 45.0%
! GATGACGTTTTTGTAGGCGA+CGG - chr5_1:43980835-43980854 None:intergenic 45.0%
GTCGCCTACAAAAACGTCAT+CGG + chr5_1:43980834-43980853 Msa0732080:CDS 45.0%
! TGCTCCGATGACGTTTTTGT+AGG - chr5_1:43980841-43980860 None:intergenic 45.0%
CATCTCTTCCATCGAACAGA+AGG + chr5_1:43980881-43980900 Msa0732080:CDS 45.0%
GCTTTCTTCCTTCTGTTCGA+TGG - chr5_1:43980892-43980911 None:intergenic 45.0%
! TTGATTCCTTCTGCTGCTTC+TGG + chr5_1:43981017-43981036 Msa0732080:CDS 45.0%
! TGCTGCTTCTGGTGATTCTA+AGG + chr5_1:43981028-43981047 Msa0732080:CDS 45.0%
CGATGAGGCTATTGCTGAAT+TGG + chr5_1:43982423-43982442 Msa0732080:CDS 45.0%
TCCAAAGGGTGAGGTTATCA+CGG - chr5_1:43982501-43982520 None:intergenic 45.0%
ATCAAAGAAGCCGCACCAAA+AGG + chr5_1:43982649-43982668 Msa0732080:CDS 45.0%
!!! TTAATTAGTTTTTTTTTTTT+TGG + chr5_1:43981429-43981448 Msa0732080:intron 5.0%
ACGAACTCTTCACGTGGTGT+TGG - chr5_1:43980693-43980712 None:intergenic 50.0%
AGCCGAACGTTACGAAGAGA+TGG + chr5_1:43980737-43980756 Msa0732080:CDS 50.0%
GGTGAGTTCTTCGCTTTCGA+CGG - chr5_1:43980790-43980809 None:intergenic 50.0%
GAGGAGGTTACGTTCTTCGA+CGG - chr5_1:43980811-43980830 None:intergenic 50.0%
GAACAGAAGGAAGAAAGCCG+TGG + chr5_1:43980894-43980913 Msa0732080:CDS 50.0%
GAATCACCAGAAGCAGCAGA+AGG - chr5_1:43981026-43981045 None:intergenic 50.0%
TACCACCAACTCACCCAATC+AGG + chr5_1:43981874-43981893 Msa0732080:CDS 50.0%
GAGCAAGACCAAGCCTGATT+GGG - chr5_1:43981890-43981909 None:intergenic 50.0%
AGAGCAAGACCAAGCCTGAT+TGG - chr5_1:43981891-43981910 None:intergenic 50.0%
AACTCTCCTGATCGTGCTTG+TGG + chr5_1:43981942-43981961 Msa0732080:CDS 50.0%
! TGCTTGTGGCCTTGCAAAAC+AGG + chr5_1:43981956-43981975 Msa0732080:CDS 50.0%
! TTGTGGGAGCTTTAGTGCAC+TGG + chr5_1:43982227-43982246 Msa0732080:intron 50.0%
! TGTGGGAGCTTTAGTGCACT+GGG + chr5_1:43982228-43982247 Msa0732080:intron 50.0%
TCCGTGATAACCTCACCCTT+TGG + chr5_1:43982497-43982516 Msa0732080:CDS 50.0%
CTGCATGTCAGAGGTCCAAA+GGG - chr5_1:43982515-43982534 None:intergenic 50.0%
!! CTCTTCACGTGGTGTTGGTG+CGG - chr5_1:43980688-43980707 None:intergenic 55.0%
GAGTTCTTCGCTTTCGACGG+CGG - chr5_1:43980787-43980806 None:intergenic 55.0%
! TTTTGTAGGCGACGGAGAGG+AGG - chr5_1:43980827-43980846 None:intergenic 55.0%
! CGTTTTTGTAGGCGACGGAG+AGG - chr5_1:43980830-43980849 None:intergenic 55.0%
! AGCCTGATTGGGTGAGTTGG+TGG - chr5_1:43981879-43981898 None:intergenic 55.0%
CCAAGCCTGATTGGGTGAGT+TGG - chr5_1:43981882-43981901 None:intergenic 55.0%
CCAACTCACCCAATCAGGCT+TGG + chr5_1:43981879-43981898 Msa0732080:CDS 55.0%
TGTCAGAGGTCCAAAGGGTG+AGG - chr5_1:43982510-43982529 None:intergenic 55.0%
CCTGCATGTCAGAGGTCCAA+AGG - chr5_1:43982516-43982535 None:intergenic 55.0%
CCTTTGGACCTCTGACATGC+AGG + chr5_1:43982513-43982532 Msa0732080:CDS 55.0%
! TACTGCTCATCGGCGCCTTT+TGG - chr5_1:43982667-43982686 None:intergenic 55.0%
GTTGGTGCGGTTGCCATGGA+TGG - chr5_1:43980675-43980694 None:intergenic 60.0%
!! TGGTGTTGGTGCGGTTGCCA+TGG - chr5_1:43980679-43980698 None:intergenic 60.0%
TTCTTCGCTTTCGACGGCGG+CGG - chr5_1:43980784-43980803 None:intergenic 60.0%
TCGGAGCAAGACGTGCTTCG+TGG + chr5_1:43980853-43980872 Msa0732080:CDS 60.0%
GCAAGGCCACAAGCACGATC+AGG - chr5_1:43981951-43981970 None:intergenic 60.0%
! CTCATCGGCGCCTTTTGGTG+CGG - chr5_1:43982662-43982681 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 43980386 43983145 43980386 ID=Msa0732080;Name=Msa0732080
chr5_1 mRNA 43980386 43983145 43980386 ID=Msa0732080-mRNA-1;Parent=Msa0732080;Name=Msa0732080-mRNA-1;_AED=0.01;_eAED=0.01;_QI=292|1|1|1|1|1|4|458|260
chr5_1 exon 43980386 43981163 43980386 ID=Msa0732080-mRNA-1:exon:12472;Parent=Msa0732080-mRNA-1
chr5_1 exon 43981855 43981977 43981855 ID=Msa0732080-mRNA-1:exon:12473;Parent=Msa0732080-mRNA-1
chr5_1 exon 43982418 43982534 43982418 ID=Msa0732080-mRNA-1:exon:12474;Parent=Msa0732080-mRNA-1
chr5_1 exon 43982631 43983145 43982631 ID=Msa0732080-mRNA-1:exon:12475;Parent=Msa0732080-mRNA-1
chr5_1 five_prime_UTR 43980386 43980677 43980386 ID=Msa0732080-mRNA-1:five_prime_utr;Parent=Msa0732080-mRNA-1
chr5_1 CDS 43980678 43981163 43980678 ID=Msa0732080-mRNA-1:cds;Parent=Msa0732080-mRNA-1
chr5_1 CDS 43981855 43981977 43981855 ID=Msa0732080-mRNA-1:cds;Parent=Msa0732080-mRNA-1
chr5_1 CDS 43982418 43982534 43982418 ID=Msa0732080-mRNA-1:cds;Parent=Msa0732080-mRNA-1
chr5_1 CDS 43982631 43982687 43982631 ID=Msa0732080-mRNA-1:cds;Parent=Msa0732080-mRNA-1
chr5_1 three_prime_UTR 43982688 43983145 43982688 ID=Msa0732080-mRNA-1:three_prime_utr;Parent=Msa0732080-mRNA-1
Gene Sequence

>Msa0732080

ATGGCAACCGCACCAACACCACGTGAAGAGTTCGTCTACATGGCGAAACTCGCCGAACAAGCCGAACGTTACGAAGAGATGGTCGATTTCATGGAAAAAGTAACCGCCGCCGTCGAAAGCGAAGAACTCACCGTCGAAGAACGTAACCTCCTCTCCGTCGCCTACAAAAACGTCATCGGAGCAAGACGTGCTTCGTGGCGTATCATCTCTTCCATCGAACAGAAGGAAGAAAGCCGTGGTAACGATGAACATGTTACCGTGATTCGTGATTACAGATCTAAGATCGAAGCTGAACTTTCCAATATCTGTAACGGTATTCTTAAGCTTCTTGATTCTCGCTTGATTCCTTCTGCTGCTTCTGGTGATTCTAAGGTTTTCTACCTTAAGATGAAAGGTGATTATCATAGGTATCTTGCTGAGTTTAAGAGTGGCGCTGAGCGTAAAGATGCTGCTGAAAGTACTCTTACTGCTTATAAAGCTGCTCAGGACATTGCTAACTCTGAGCTACCACCAACTCACCCAATCAGGCTTGGTCTTGCTCTGAACTTCTCAGTGTTTTACTATGAGATTCTTAACTCTCCTGATCGTGCTTGTGGCCTTGCAAAACAGGCTTTCGATGAGGCTATTGCTGAATTGGATACATTGGGAGAGGAATCATACAAGGACAGCACTTTGATCATGCAACTTCTCCGTGATAACCTCACCCTTTGGACCTCTGACATGCAGGATGATGGTGCAGATGAAATCAAAGAAGCCGCACCAAAAGGCGCCGATGAGCAGTAA
Protein sequence

>Msa0732080

MATAPTPREEFVYMAKLAEQAERYEEMVDFMEKVTAAVESEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDEHVTVIRDYRSKIEAELSNICNGILKLLDSRLIPSAASGDSKVFYLKMKGDYHRYLAEFKSGAERKDAAESTLTAYKAAQDIANSELPPTHPIRLGLALNFSVFYYEILNSPDRACGLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDDGADEIKEAAPKGADEQ*