AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0732420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732420 MtrunA17_Chr5g0447991 91.795 195 16 0 1 195 1 195 9.76e-80 236
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTAAATAATGCTTATTT+TGG 0.137891 5_1:-44827835 None:intergenic
CTTTGTATGATTATGCATTT+TGG 0.148495 5_1:+44829681 Msa0732420:three_prime_UTR
TTCAGCTGAAGATTCTTCTT+TGG 0.248746 5_1:-44828984 None:intergenic
CCAATTGAGAAGAGTGAATA+AGG 0.253557 5_1:+44829421 Msa0732420:CDS
ACCTCAACAGTGGCCATAAT+TGG 0.274025 5_1:-44828096 None:intergenic
CCTTATTCACTCTTCTCAAT+TGG 0.285248 5_1:-44829421 None:intergenic
TTCTGTTGTTGAGGCTGTTC+AGG 0.288375 5_1:+44829186 Msa0732420:CDS
TCTGATGCTGCTGGTGTGAT+TGG 0.302513 5_1:-44828959 None:intergenic
ACGTTTACATAAACTCTCTT+TGG 0.330189 5_1:+44829772 Msa0732420:three_prime_UTR
AAATGCATGGGTAGAAGATA+TGG 0.333565 5_1:+44829603 Msa0732420:three_prime_UTR
GTCGAGGAAACAAAAGAAAA+TGG 0.336894 5_1:+44829160 Msa0732420:CDS
TCTATTGGGAGAATGAATTA+TGG 0.354702 5_1:-44827807 None:intergenic
ATTACTGAAAAGGTTACATT+AGG 0.365107 5_1:-44829652 None:intergenic
GTAAGCATGTTTGGCAATAA+TGG 0.367425 5_1:-44827888 None:intergenic
CTCAACAGGTTCCTTTGTCT+CGG 0.374352 5_1:-44829107 None:intergenic
TTACATTAGGTAAATTCAAA+TGG 0.389874 5_1:-44829639 None:intergenic
TCTGTCTCAGATTCTGCAGC+TGG 0.400444 5_1:-44829214 None:intergenic
CTCAGCTTTAACCAAAGGTT+TGG 0.405277 5_1:-44829011 None:intergenic
GCTTACTAGCATTCAGTGAA+TGG 0.421591 5_1:+44827905 Msa0732420:five_prime_UTR
ATTTAGAATGGTTTATATAG+AGG 0.427250 5_1:-44827994 None:intergenic
AGCTGAAGAAGCCAAACCTT+TGG 0.430085 5_1:+44829000 Msa0732420:CDS
TGAGACAGAAAATGTTGTTA+AGG 0.433126 5_1:+44829228 Msa0732420:CDS
TGAATGCTAGTAAGCATGTT+TGG 0.437182 5_1:-44827897 None:intergenic
GGTAGCTGAGGCTGAAACTA+AGG 0.438659 5_1:+44829261 Msa0732420:CDS
GGTTGAGAAAGAAGAAGCCT+TGG 0.446199 5_1:+44829330 Msa0732420:CDS
AACAAAGTTAAGATTGCTAT+AGG 0.461132 5_1:-44828051 None:intergenic
TCTTTGGTTTCTGATGCTGC+TGG 0.462673 5_1:-44828968 None:intergenic
CTTACTAGCATTCAGTGAAT+GGG 0.465281 5_1:+44827906 Msa0732420:five_prime_UTR
AAAGGAACATATTACTGAAA+AGG 0.467777 5_1:-44829662 None:intergenic
AGTTTATGTAAACGTGACTA+AGG 0.472999 5_1:-44829764 None:intergenic
TGATAACTGAGCAGTGTCTT+CGG 0.478773 5_1:-44828894 None:intergenic
TACATTAGGTAAATTCAAAT+GGG 0.480670 5_1:-44829638 None:intergenic
TGGTTTATATAGAGGTTTGA+GGG 0.484870 5_1:-44827986 None:intergenic
CAATTGAGAAGAGTGAATAA+GGG 0.495888 5_1:+44829422 Msa0732420:CDS
TCCAATTATGGCCACTGTTG+AGG 0.502667 5_1:+44828095 Msa0732420:exon
TACAGCAGAGGAAACAAAGA+AGG 0.515632 5_1:+44829075 Msa0732420:CDS
AATTGAGAAGAGTGAAGAAG+AGG 0.516526 5_1:+44829396 Msa0732420:CDS
GGTTAAAGCTGAGGCAAAAG+AGG 0.520787 5_1:+44829021 Msa0732420:CDS
ATGGTTTATATAGAGGTTTG+AGG 0.531817 5_1:-44827987 None:intergenic
AAGTGCTTAATTAGAGAGAG+TGG 0.533334 5_1:+44829466 Msa0732420:three_prime_UTR
GGAAGAACAAACCGAGACAA+AGG 0.535864 5_1:+44829096 Msa0732420:CDS
AGAAGAGAAAAGTGAGGAGA+AGG 0.537941 5_1:+44829309 Msa0732420:CDS
TTTGCCTCAGCTTTAACCAA+AGG 0.538136 5_1:-44829016 None:intergenic
GTTGAGGAAACAAAAGAAAA+CGG 0.538839 5_1:+44829124 Msa0732420:CDS
AATGCATGGGTAGAAGATAT+GGG 0.541191 5_1:+44829604 Msa0732420:three_prime_UTR
ACTCTCAACGATTTCTTCCG+TGG 0.558062 5_1:-44828936 None:intergenic
AGCCTTGGATGAAAAGAAAG+AGG 0.562181 5_1:+44829345 Msa0732420:CDS
TGATGAAAGAAATGAGGTGG+GGG 0.570922 5_1:-44827956 None:intergenic
CGAGACAAAGGAACCTGTTG+AGG 0.574462 5_1:+44829108 Msa0732420:CDS
CAAACCTTTGGTTAAAGCTG+AGG 0.582591 5_1:+44829012 Msa0732420:CDS
AATTGAGAAGAGTGAATAAG+GGG 0.602175 5_1:+44829423 Msa0732420:exon
AGTTGATGAAAGAAATGAGG+TGG 0.608604 5_1:-44827959 None:intergenic
AGCTTAGAGATGAGGTAACA+AGG 0.612100 5_1:-44827858 None:intergenic
AAATGGTGATTCTGTTGTTG+AGG 0.613322 5_1:+44829177 Msa0732420:CDS
GTGGCAAAAGCTTAGAGATG+AGG 0.617154 5_1:-44827866 None:intergenic
ACTACTAGTAGCTAAATGCA+TGG 0.619835 5_1:+44829590 Msa0732420:three_prime_UTR
CTACTAGTAGCTAAATGCAT+GGG 0.620080 5_1:+44829591 Msa0732420:three_prime_UTR
GTTTATGTAAACGTGACTAA+GGG 0.620583 5_1:-44829763 None:intergenic
TGTTGTTAAGGATGAGAATG+TGG 0.621253 5_1:+44829240 Msa0732420:CDS
GTTGATGAAAGAAATGAGGT+GGG 0.621816 5_1:-44827958 None:intergenic
CGGTGATTCAGTTAATGTCG+AGG 0.622446 5_1:+44829144 Msa0732420:CDS
ACAAGTTGATGAAAGAAATG+AGG 0.623867 5_1:-44827962 None:intergenic
CAAAATGCATAATCATACAA+AGG 0.624630 5_1:-44829680 None:intergenic
AGCCACAGAAGAGAAAAGTG+AGG 0.638283 5_1:+44829303 Msa0732420:CDS
AGCATGTTTGGCAATAATGG+TGG 0.642031 5_1:-44827885 None:intergenic
TTACTAGCATTCAGTGAATG+GGG 0.664936 5_1:+44827907 Msa0732420:five_prime_UTR
GGATGAGAATGTGGTAGCTG+AGG 0.670593 5_1:+44829249 Msa0732420:CDS
GACTGTGAAAGATACAGCAG+AGG 0.672134 5_1:+44829063 Msa0732420:CDS
TAAAGCTGAGGCAAAAGAGG+TGG 0.676484 5_1:+44829024 Msa0732420:CDS
TTGATGAAAGAAATGAGGTG+GGG 0.683097 5_1:-44827957 None:intergenic
TGGTAGAGTTACCTCAACAG+TGG 0.712754 5_1:-44828106 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAATAATGCTTATTT+TGG - chr5_1:44827838-44827857 None:intergenic 10.0%
!!! TTAATTTTGATGAAAAATAA+TGG - chr5_1:44828021-44828040 None:intergenic 10.0%
!!! ATGGAAATTTTAATTATAAT+TGG + chr5_1:44828528-44828547 Msa0732420:intron 10.0%
!!! TTATTAAATAATGCTTATTT+TGG - chr5_1:44827838-44827857 None:intergenic 10.0%
!!! TTAATTTTGATGAAAAATAA+TGG - chr5_1:44828021-44828040 None:intergenic 10.0%
!!! ATGGAAATTTTAATTATAAT+TGG + chr5_1:44828528-44828547 Msa0732420:intron 10.0%
!!! AAAAATAATGGAATTTAGAA+TGG - chr5_1:44828009-44828028 None:intergenic 15.0%
!!! AATTTTGAGTCTTTTATTTT+TGG + chr5_1:44828633-44828652 Msa0732420:intron 15.0%
!!! AGATAATTTTTTCTTATGAA+CGG - chr5_1:44828670-44828689 None:intergenic 15.0%
!!! AAAAATAATGGAATTTAGAA+TGG - chr5_1:44828009-44828028 None:intergenic 15.0%
!!! AATTTTGAGTCTTTTATTTT+TGG + chr5_1:44828633-44828652 Msa0732420:intron 15.0%
!!! AGATAATTTTTTCTTATGAA+CGG - chr5_1:44828670-44828689 None:intergenic 15.0%
!!! ATTTAGAATGGTTTATATAG+AGG - chr5_1:44827997-44828016 None:intergenic 20.0%
!!! TTTTCTTTGTATTCCAATTA+TGG + chr5_1:44828083-44828102 Msa0732420:exon 20.0%
!!! AATCAGTGTTTAAAATCAAA+TGG - chr5_1:44828178-44828197 None:intergenic 20.0%
!!! AATATTTTAAAGCATCTAGT+AGG + chr5_1:44828212-44828231 Msa0732420:intron 20.0%
!! AACATCTAATGCATTAAAAA+AGG - chr5_1:44828398-44828417 None:intergenic 20.0%
!!! AAAATTTGAAGCACTTAAAA+TGG + chr5_1:44828509-44828528 Msa0732420:intron 20.0%
!!! GATAATTTTTTCTTATGAAC+GGG - chr5_1:44828669-44828688 None:intergenic 20.0%
!! AGTAAAATCTGAACAAAAAT+TGG + chr5_1:44828719-44828738 Msa0732420:intron 20.0%
!! TACATTAGGTAAATTCAAAT+GGG - chr5_1:44829641-44829660 None:intergenic 20.0%
!! TTACATTAGGTAAATTCAAA+TGG - chr5_1:44829642-44829661 None:intergenic 20.0%
!!! ATTTAGAATGGTTTATATAG+AGG - chr5_1:44827997-44828016 None:intergenic 20.0%
!!! TTTTCTTTGTATTCCAATTA+TGG + chr5_1:44828083-44828102 Msa0732420:exon 20.0%
!!! AATCAGTGTTTAAAATCAAA+TGG - chr5_1:44828178-44828197 None:intergenic 20.0%
!!! AATATTTTAAAGCATCTAGT+AGG + chr5_1:44828212-44828231 Msa0732420:intron 20.0%
!! AACATCTAATGCATTAAAAA+AGG - chr5_1:44828398-44828417 None:intergenic 20.0%
!!! AAAATTTGAAGCACTTAAAA+TGG + chr5_1:44828509-44828528 Msa0732420:intron 20.0%
!!! GATAATTTTTTCTTATGAAC+GGG - chr5_1:44828669-44828688 None:intergenic 20.0%
!! AGTAAAATCTGAACAAAAAT+TGG + chr5_1:44828719-44828738 Msa0732420:intron 20.0%
!! TACATTAGGTAAATTCAAAT+GGG - chr5_1:44829641-44829660 None:intergenic 20.0%
!! TTACATTAGGTAAATTCAAA+TGG - chr5_1:44829642-44829661 None:intergenic 20.0%
!!! TTATTTTGGATCACTCTATT+GGG - chr5_1:44827824-44827843 None:intergenic 25.0%
! AACAAAGTTAAGATTGCTAT+AGG - chr5_1:44828054-44828073 None:intergenic 25.0%
! AAAAACCAAGTACTTGAAAT+TGG - chr5_1:44828129-44828148 None:intergenic 25.0%
!!! CATCAATATTTTAATGTCTC+TGG - chr5_1:44828374-44828393 None:intergenic 25.0%
! AAAGAAAGAAATGCATTAGA+TGG + chr5_1:44828426-44828445 Msa0732420:intron 25.0%
! TCATATCTTCTTGAGTAAAT+AGG - chr5_1:44828475-44828494 None:intergenic 25.0%
!! CAAATGTTGTTTATTAGACA+AGG - chr5_1:44828822-44828841 None:intergenic 25.0%
!!! TTTTCTTGTTACTTGTTTAG+TGG + chr5_1:44829543-44829562 Msa0732420:three_prime_UTR 25.0%
! ATTACTGAAAAGGTTACATT+AGG - chr5_1:44829655-44829674 None:intergenic 25.0%
! AAAGGAACATATTACTGAAA+AGG - chr5_1:44829665-44829684 None:intergenic 25.0%
! CAAAATGCATAATCATACAA+AGG - chr5_1:44829683-44829702 None:intergenic 25.0%
!! CTTTGTATGATTATGCATTT+TGG + chr5_1:44829681-44829700 Msa0732420:three_prime_UTR 25.0%
!!! TTATTTTGGATCACTCTATT+GGG - chr5_1:44827824-44827843 None:intergenic 25.0%
! AACAAAGTTAAGATTGCTAT+AGG - chr5_1:44828054-44828073 None:intergenic 25.0%
! AAAAACCAAGTACTTGAAAT+TGG - chr5_1:44828129-44828148 None:intergenic 25.0%
!!! CATCAATATTTTAATGTCTC+TGG - chr5_1:44828374-44828393 None:intergenic 25.0%
! AAAGAAAGAAATGCATTAGA+TGG + chr5_1:44828426-44828445 Msa0732420:intron 25.0%
! TCATATCTTCTTGAGTAAAT+AGG - chr5_1:44828475-44828494 None:intergenic 25.0%
!! CAAATGTTGTTTATTAGACA+AGG - chr5_1:44828822-44828841 None:intergenic 25.0%
!!! TTTTCTTGTTACTTGTTTAG+TGG + chr5_1:44829543-44829562 Msa0732420:three_prime_UTR 25.0%
! ATTACTGAAAAGGTTACATT+AGG - chr5_1:44829655-44829674 None:intergenic 25.0%
! AAAGGAACATATTACTGAAA+AGG - chr5_1:44829665-44829684 None:intergenic 25.0%
! CAAAATGCATAATCATACAA+AGG - chr5_1:44829683-44829702 None:intergenic 25.0%
!! CTTTGTATGATTATGCATTT+TGG + chr5_1:44829681-44829700 Msa0732420:three_prime_UTR 25.0%
TCTATTGGGAGAATGAATTA+TGG - chr5_1:44827810-44827829 None:intergenic 30.0%
!!! CTTATTTTGGATCACTCTAT+TGG - chr5_1:44827825-44827844 None:intergenic 30.0%
!! ACAAGTTGATGAAAGAAATG+AGG - chr5_1:44827965-44827984 None:intergenic 30.0%
TGGTTTATATAGAGGTTTGA+GGG - chr5_1:44827989-44828008 None:intergenic 30.0%
ATGGTTTATATAGAGGTTTG+AGG - chr5_1:44827990-44828009 None:intergenic 30.0%
GTGATTAAAACATGTCATGT+GGG - chr5_1:44828275-44828294 None:intergenic 30.0%
!!! CACATGACATGTTTTAATCA+CGG + chr5_1:44828274-44828293 Msa0732420:intron 30.0%
ATCTTCTTGAGTAAATAGGA+AGG - chr5_1:44828471-44828490 None:intergenic 30.0%
!!! ATTTTTTCTTATGAACGGGA+CGG - chr5_1:44828665-44828684 None:intergenic 30.0%
TTTAGATAAGATTCGAACTC+AGG + chr5_1:44828742-44828761 Msa0732420:intron 30.0%
! TGATCTGAATATGATTTTGC+AGG + chr5_1:44828847-44828866 Msa0732420:intron 30.0%
GAAATCTGATGAAAAAACCA+CGG + chr5_1:44828919-44828938 Msa0732420:CDS 30.0%
!! TTTTCTTTTGTTTCCTCAAC+AGG - chr5_1:44829124-44829143 None:intergenic 30.0%
GTTGAGGAAACAAAAGAAAA+CGG + chr5_1:44829124-44829143 Msa0732420:CDS 30.0%
TGAGACAGAAAATGTTGTTA+AGG + chr5_1:44829228-44829247 Msa0732420:CDS 30.0%
! CTCAATTTTTGCATCATTGT+TGG - chr5_1:44829383-44829402 None:intergenic 30.0%
CAATTGAGAAGAGTGAATAA+GGG + chr5_1:44829422-44829441 Msa0732420:CDS 30.0%
AATTGAGAAGAGTGAATAAG+GGG + chr5_1:44829423-44829442 Msa0732420:exon 30.0%
GTTTATGTAAACGTGACTAA+GGG - chr5_1:44829766-44829785 None:intergenic 30.0%
AGTTTATGTAAACGTGACTA+AGG - chr5_1:44829767-44829786 None:intergenic 30.0%
TCTATTGGGAGAATGAATTA+TGG - chr5_1:44827810-44827829 None:intergenic 30.0%
!!! CTTATTTTGGATCACTCTAT+TGG - chr5_1:44827825-44827844 None:intergenic 30.0%
!! ACAAGTTGATGAAAGAAATG+AGG - chr5_1:44827965-44827984 None:intergenic 30.0%
TGGTTTATATAGAGGTTTGA+GGG - chr5_1:44827989-44828008 None:intergenic 30.0%
ATGGTTTATATAGAGGTTTG+AGG - chr5_1:44827990-44828009 None:intergenic 30.0%
GTGATTAAAACATGTCATGT+GGG - chr5_1:44828275-44828294 None:intergenic 30.0%
!!! CACATGACATGTTTTAATCA+CGG + chr5_1:44828274-44828293 Msa0732420:intron 30.0%
ATCTTCTTGAGTAAATAGGA+AGG - chr5_1:44828471-44828490 None:intergenic 30.0%
!!! ATTTTTTCTTATGAACGGGA+CGG - chr5_1:44828665-44828684 None:intergenic 30.0%
TTTAGATAAGATTCGAACTC+AGG + chr5_1:44828742-44828761 Msa0732420:intron 30.0%
! TGATCTGAATATGATTTTGC+AGG + chr5_1:44828847-44828866 Msa0732420:intron 30.0%
GAAATCTGATGAAAAAACCA+CGG + chr5_1:44828919-44828938 Msa0732420:CDS 30.0%
!! TTTTCTTTTGTTTCCTCAAC+AGG - chr5_1:44829124-44829143 None:intergenic 30.0%
GTTGAGGAAACAAAAGAAAA+CGG + chr5_1:44829124-44829143 Msa0732420:CDS 30.0%
TGAGACAGAAAATGTTGTTA+AGG + chr5_1:44829228-44829247 Msa0732420:CDS 30.0%
! CTCAATTTTTGCATCATTGT+TGG - chr5_1:44829383-44829402 None:intergenic 30.0%
CAATTGAGAAGAGTGAATAA+GGG + chr5_1:44829422-44829441 Msa0732420:CDS 30.0%
AATTGAGAAGAGTGAATAAG+GGG + chr5_1:44829423-44829442 Msa0732420:exon 30.0%
GTTTATGTAAACGTGACTAA+GGG - chr5_1:44829766-44829785 None:intergenic 30.0%
AGTTTATGTAAACGTGACTA+AGG - chr5_1:44829767-44829786 None:intergenic 30.0%
GTAAGCATGTTTGGCAATAA+TGG - chr5_1:44827891-44827910 None:intergenic 35.0%
TGAATGCTAGTAAGCATGTT+TGG - chr5_1:44827900-44827919 None:intergenic 35.0%
CTTACTAGCATTCAGTGAAT+GGG + chr5_1:44827906-44827925 Msa0732420:five_prime_UTR 35.0%
TTACTAGCATTCAGTGAATG+GGG + chr5_1:44827907-44827926 Msa0732420:five_prime_UTR 35.0%
TTGATGAAAGAAATGAGGTG+GGG - chr5_1:44827960-44827979 None:intergenic 35.0%
! GTTGATGAAAGAAATGAGGT+GGG - chr5_1:44827961-44827980 None:intergenic 35.0%
!! AGTTGATGAAAGAAATGAGG+TGG - chr5_1:44827962-44827981 None:intergenic 35.0%
! CTCTACCAATTTCAAGTACT+TGG + chr5_1:44828121-44828140 Msa0732420:intron 35.0%
CGTGATTAAAACATGTCATG+TGG - chr5_1:44828276-44828295 None:intergenic 35.0%
ACTCTGACTAATATGACACA+TGG - chr5_1:44828598-44828617 None:intergenic 35.0%
!! GTAAACTCTGAAGTTCGATT+AGG + chr5_1:44828691-44828710 Msa0732420:intron 35.0%
TTCAGCTGAAGATTCTTCTT+TGG - chr5_1:44828987-44829006 None:intergenic 35.0%
GTCGAGGAAACAAAAGAAAA+TGG + chr5_1:44829160-44829179 Msa0732420:CDS 35.0%
AAATGGTGATTCTGTTGTTG+AGG + chr5_1:44829177-44829196 Msa0732420:CDS 35.0%
TGTTGTTAAGGATGAGAATG+TGG + chr5_1:44829240-44829259 Msa0732420:CDS 35.0%
! TTCCTCTTTCTTTTCATCCA+AGG - chr5_1:44829350-44829369 None:intergenic 35.0%
AATTGAGAAGAGTGAAGAAG+AGG + chr5_1:44829396-44829415 Msa0732420:CDS 35.0%
CCTTATTCACTCTTCTCAAT+TGG - chr5_1:44829424-44829443 None:intergenic 35.0%
CCAATTGAGAAGAGTGAATA+AGG + chr5_1:44829421-44829440 Msa0732420:CDS 35.0%
AAGTGCTTAATTAGAGAGAG+TGG + chr5_1:44829466-44829485 Msa0732420:three_prime_UTR 35.0%
ACTACTAGTAGCTAAATGCA+TGG + chr5_1:44829590-44829609 Msa0732420:three_prime_UTR 35.0%
CTACTAGTAGCTAAATGCAT+GGG + chr5_1:44829591-44829610 Msa0732420:three_prime_UTR 35.0%
AAATGCATGGGTAGAAGATA+TGG + chr5_1:44829603-44829622 Msa0732420:three_prime_UTR 35.0%
AATGCATGGGTAGAAGATAT+GGG + chr5_1:44829604-44829623 Msa0732420:three_prime_UTR 35.0%
GTAAGCATGTTTGGCAATAA+TGG - chr5_1:44827891-44827910 None:intergenic 35.0%
TGAATGCTAGTAAGCATGTT+TGG - chr5_1:44827900-44827919 None:intergenic 35.0%
CTTACTAGCATTCAGTGAAT+GGG + chr5_1:44827906-44827925 Msa0732420:five_prime_UTR 35.0%
TTACTAGCATTCAGTGAATG+GGG + chr5_1:44827907-44827926 Msa0732420:five_prime_UTR 35.0%
TTGATGAAAGAAATGAGGTG+GGG - chr5_1:44827960-44827979 None:intergenic 35.0%
! GTTGATGAAAGAAATGAGGT+GGG - chr5_1:44827961-44827980 None:intergenic 35.0%
!! AGTTGATGAAAGAAATGAGG+TGG - chr5_1:44827962-44827981 None:intergenic 35.0%
! CTCTACCAATTTCAAGTACT+TGG + chr5_1:44828121-44828140 Msa0732420:intron 35.0%
CGTGATTAAAACATGTCATG+TGG - chr5_1:44828276-44828295 None:intergenic 35.0%
ACTCTGACTAATATGACACA+TGG - chr5_1:44828598-44828617 None:intergenic 35.0%
!! GTAAACTCTGAAGTTCGATT+AGG + chr5_1:44828691-44828710 Msa0732420:intron 35.0%
TTCAGCTGAAGATTCTTCTT+TGG - chr5_1:44828987-44829006 None:intergenic 35.0%
GTCGAGGAAACAAAAGAAAA+TGG + chr5_1:44829160-44829179 Msa0732420:CDS 35.0%
AAATGGTGATTCTGTTGTTG+AGG + chr5_1:44829177-44829196 Msa0732420:CDS 35.0%
TGTTGTTAAGGATGAGAATG+TGG + chr5_1:44829240-44829259 Msa0732420:CDS 35.0%
! TTCCTCTTTCTTTTCATCCA+AGG - chr5_1:44829350-44829369 None:intergenic 35.0%
AATTGAGAAGAGTGAAGAAG+AGG + chr5_1:44829396-44829415 Msa0732420:CDS 35.0%
CCTTATTCACTCTTCTCAAT+TGG - chr5_1:44829424-44829443 None:intergenic 35.0%
CCAATTGAGAAGAGTGAATA+AGG + chr5_1:44829421-44829440 Msa0732420:CDS 35.0%
AAGTGCTTAATTAGAGAGAG+TGG + chr5_1:44829466-44829485 Msa0732420:three_prime_UTR 35.0%
ACTACTAGTAGCTAAATGCA+TGG + chr5_1:44829590-44829609 Msa0732420:three_prime_UTR 35.0%
CTACTAGTAGCTAAATGCAT+GGG + chr5_1:44829591-44829610 Msa0732420:three_prime_UTR 35.0%
AAATGCATGGGTAGAAGATA+TGG + chr5_1:44829603-44829622 Msa0732420:three_prime_UTR 35.0%
AATGCATGGGTAGAAGATAT+GGG + chr5_1:44829604-44829623 Msa0732420:three_prime_UTR 35.0%
AGCTTAGAGATGAGGTAACA+AGG - chr5_1:44827861-44827880 None:intergenic 40.0%
AGCATGTTTGGCAATAATGG+TGG - chr5_1:44827888-44827907 None:intergenic 40.0%
GCTTACTAGCATTCAGTGAA+TGG + chr5_1:44827905-44827924 Msa0732420:five_prime_UTR 40.0%
TGATGAAAGAAATGAGGTGG+GGG - chr5_1:44827959-44827978 None:intergenic 40.0%
!! AACTCTGAAGTTCGATTAGG+AGG + chr5_1:44828694-44828713 Msa0732420:intron 40.0%
TGATAACTGAGCAGTGTCTT+CGG - chr5_1:44828897-44828916 None:intergenic 40.0%
! CTCAGCTTTAACCAAAGGTT+TGG - chr5_1:44829014-44829033 None:intergenic 40.0%
CAAACCTTTGGTTAAAGCTG+AGG + chr5_1:44829012-44829031 Msa0732420:CDS 40.0%
! TTTGCCTCAGCTTTAACCAA+AGG - chr5_1:44829019-44829038 None:intergenic 40.0%
TACAGCAGAGGAAACAAAGA+AGG + chr5_1:44829075-44829094 Msa0732420:CDS 40.0%
AGAAGAGAAAAGTGAGGAGA+AGG + chr5_1:44829309-44829328 Msa0732420:CDS 40.0%
AGCCTTGGATGAAAAGAAAG+AGG + chr5_1:44829345-44829364 Msa0732420:CDS 40.0%
AGCTTAGAGATGAGGTAACA+AGG - chr5_1:44827861-44827880 None:intergenic 40.0%
AGCATGTTTGGCAATAATGG+TGG - chr5_1:44827888-44827907 None:intergenic 40.0%
GCTTACTAGCATTCAGTGAA+TGG + chr5_1:44827905-44827924 Msa0732420:five_prime_UTR 40.0%
TGATGAAAGAAATGAGGTGG+GGG - chr5_1:44827959-44827978 None:intergenic 40.0%
!! AACTCTGAAGTTCGATTAGG+AGG + chr5_1:44828694-44828713 Msa0732420:intron 40.0%
TGATAACTGAGCAGTGTCTT+CGG - chr5_1:44828897-44828916 None:intergenic 40.0%
! CTCAGCTTTAACCAAAGGTT+TGG - chr5_1:44829014-44829033 None:intergenic 40.0%
CAAACCTTTGGTTAAAGCTG+AGG + chr5_1:44829012-44829031 Msa0732420:CDS 40.0%
! TTTGCCTCAGCTTTAACCAA+AGG - chr5_1:44829019-44829038 None:intergenic 40.0%
TACAGCAGAGGAAACAAAGA+AGG + chr5_1:44829075-44829094 Msa0732420:CDS 40.0%
AGAAGAGAAAAGTGAGGAGA+AGG + chr5_1:44829309-44829328 Msa0732420:CDS 40.0%
AGCCTTGGATGAAAAGAAAG+AGG + chr5_1:44829345-44829364 Msa0732420:CDS 40.0%
GTGGCAAAAGCTTAGAGATG+AGG - chr5_1:44827869-44827888 None:intergenic 45.0%
TCCAATTATGGCCACTGTTG+AGG + chr5_1:44828095-44828114 Msa0732420:exon 45.0%
ACCTCAACAGTGGCCATAAT+TGG - chr5_1:44828099-44828118 None:intergenic 45.0%
TGGTAGAGTTACCTCAACAG+TGG - chr5_1:44828109-44828128 None:intergenic 45.0%
TGTCATGTGGGAGTAATGCA+GGG - chr5_1:44828263-44828282 None:intergenic 45.0%
ATGTCATGTGGGAGTAATGC+AGG - chr5_1:44828264-44828283 None:intergenic 45.0%
ACTCTCAACGATTTCTTCCG+TGG - chr5_1:44828939-44828958 None:intergenic 45.0%
! TCTTTGGTTTCTGATGCTGC+TGG - chr5_1:44828971-44828990 None:intergenic 45.0%
AGCTGAAGAAGCCAAACCTT+TGG + chr5_1:44829000-44829019 Msa0732420:CDS 45.0%
GGTTAAAGCTGAGGCAAAAG+AGG + chr5_1:44829021-44829040 Msa0732420:CDS 45.0%
TAAAGCTGAGGCAAAAGAGG+TGG + chr5_1:44829024-44829043 Msa0732420:CDS 45.0%
GACTGTGAAAGATACAGCAG+AGG + chr5_1:44829063-44829082 Msa0732420:CDS 45.0%
GGAAGAACAAACCGAGACAA+AGG + chr5_1:44829096-44829115 Msa0732420:CDS 45.0%
CTCAACAGGTTCCTTTGTCT+CGG - chr5_1:44829110-44829129 None:intergenic 45.0%
CGGTGATTCAGTTAATGTCG+AGG + chr5_1:44829144-44829163 Msa0732420:CDS 45.0%
TTCTGTTGTTGAGGCTGTTC+AGG + chr5_1:44829186-44829205 Msa0732420:CDS 45.0%
AGCCACAGAAGAGAAAAGTG+AGG + chr5_1:44829303-44829322 Msa0732420:CDS 45.0%
!! CTCCTCACTTTTCTCTTCTG+TGG - chr5_1:44829308-44829327 None:intergenic 45.0%
GGTTGAGAAAGAAGAAGCCT+TGG + chr5_1:44829330-44829349 Msa0732420:CDS 45.0%
GTGGCAAAAGCTTAGAGATG+AGG - chr5_1:44827869-44827888 None:intergenic 45.0%
TCCAATTATGGCCACTGTTG+AGG + chr5_1:44828095-44828114 Msa0732420:exon 45.0%
ACCTCAACAGTGGCCATAAT+TGG - chr5_1:44828099-44828118 None:intergenic 45.0%
TGGTAGAGTTACCTCAACAG+TGG - chr5_1:44828109-44828128 None:intergenic 45.0%
TGTCATGTGGGAGTAATGCA+GGG - chr5_1:44828263-44828282 None:intergenic 45.0%
ATGTCATGTGGGAGTAATGC+AGG - chr5_1:44828264-44828283 None:intergenic 45.0%
ACTCTCAACGATTTCTTCCG+TGG - chr5_1:44828939-44828958 None:intergenic 45.0%
! TCTTTGGTTTCTGATGCTGC+TGG - chr5_1:44828971-44828990 None:intergenic 45.0%
AGCTGAAGAAGCCAAACCTT+TGG + chr5_1:44829000-44829019 Msa0732420:CDS 45.0%
GGTTAAAGCTGAGGCAAAAG+AGG + chr5_1:44829021-44829040 Msa0732420:CDS 45.0%
TAAAGCTGAGGCAAAAGAGG+TGG + chr5_1:44829024-44829043 Msa0732420:CDS 45.0%
GACTGTGAAAGATACAGCAG+AGG + chr5_1:44829063-44829082 Msa0732420:CDS 45.0%
GGAAGAACAAACCGAGACAA+AGG + chr5_1:44829096-44829115 Msa0732420:CDS 45.0%
CTCAACAGGTTCCTTTGTCT+CGG - chr5_1:44829110-44829129 None:intergenic 45.0%
CGGTGATTCAGTTAATGTCG+AGG + chr5_1:44829144-44829163 Msa0732420:CDS 45.0%
TTCTGTTGTTGAGGCTGTTC+AGG + chr5_1:44829186-44829205 Msa0732420:CDS 45.0%
AGCCACAGAAGAGAAAAGTG+AGG + chr5_1:44829303-44829322 Msa0732420:CDS 45.0%
!! CTCCTCACTTTTCTCTTCTG+TGG - chr5_1:44829308-44829327 None:intergenic 45.0%
GGTTGAGAAAGAAGAAGCCT+TGG + chr5_1:44829330-44829349 Msa0732420:CDS 45.0%
GGGGGCAGTTCAAGAATCTT+GGG - chr5_1:44827941-44827960 None:intergenic 50.0%
TGGGGGCAGTTCAAGAATCT+TGG - chr5_1:44827942-44827961 None:intergenic 50.0%
TCTGATGCTGCTGGTGTGAT+TGG - chr5_1:44828962-44828981 None:intergenic 50.0%
CGAGACAAAGGAACCTGTTG+AGG + chr5_1:44829108-44829127 Msa0732420:CDS 50.0%
TCTGTCTCAGATTCTGCAGC+TGG - chr5_1:44829217-44829236 None:intergenic 50.0%
GGATGAGAATGTGGTAGCTG+AGG + chr5_1:44829249-44829268 Msa0732420:CDS 50.0%
GGTAGCTGAGGCTGAAACTA+AGG + chr5_1:44829261-44829280 Msa0732420:CDS 50.0%
GGGGGCAGTTCAAGAATCTT+GGG - chr5_1:44827941-44827960 None:intergenic 50.0%
TGGGGGCAGTTCAAGAATCT+TGG - chr5_1:44827942-44827961 None:intergenic 50.0%
TCTGATGCTGCTGGTGTGAT+TGG - chr5_1:44828962-44828981 None:intergenic 50.0%
CGAGACAAAGGAACCTGTTG+AGG + chr5_1:44829108-44829127 Msa0732420:CDS 50.0%
TCTGTCTCAGATTCTGCAGC+TGG - chr5_1:44829217-44829236 None:intergenic 50.0%
GGATGAGAATGTGGTAGCTG+AGG + chr5_1:44829249-44829268 Msa0732420:CDS 50.0%
GGTAGCTGAGGCTGAAACTA+AGG + chr5_1:44829261-44829280 Msa0732420:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 44827794 44829791 44827794 ID=Msa0732420;Name=Msa0732420
chr5_1 mRNA 44827794 44829791 44827794 ID=Msa0732420-mRNA-1;Parent=Msa0732420;Name=Msa0732420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=308|1|1|1|1|1|2|350|195
chr5_1 exon 44827794 44828116 44827794 ID=Msa0732420-mRNA-1:exon:12698;Parent=Msa0732420-mRNA-1
chr5_1 exon 44828869 44829791 44828869 ID=Msa0732420-mRNA-1:exon:12699;Parent=Msa0732420-mRNA-1
chr5_1 five_prime_UTR 44827794 44828101 44827794 ID=Msa0732420-mRNA-1:five_prime_utr;Parent=Msa0732420-mRNA-1
chr5_1 CDS 44828102 44828116 44828102 ID=Msa0732420-mRNA-1:cds;Parent=Msa0732420-mRNA-1
chr5_1 CDS 44828869 44829441 44828869 ID=Msa0732420-mRNA-1:cds;Parent=Msa0732420-mRNA-1
chr5_1 three_prime_UTR 44829442 44829791 44829442 ID=Msa0732420-mRNA-1:three_prime_utr;Parent=Msa0732420-mRNA-1
Gene Sequence

>Msa0732420

ATGGCCACTGTTGAGGTTGTATCAGCACAGACTGCACTCTCCGAAGACACTGCTCAGTTATCATTGAAATCTGATGAAAAAACCACGGAAGAAATCGTTGAGAGTCCAATCACACCAGCAGCATCAGAAACCAAAGAAGAATCTTCAGCTGAAGAAGCCAAACCTTTGGTTAAAGCTGAGGCAAAAGAGGTGGTAGAAGAAGTTGTTGTGACTGTGAAAGATACAGCAGAGGAAACAAAGAAGGAAGAACAAACCGAGACAAAGGAACCTGTTGAGGAAACAAAAGAAAACGGTGATTCAGTTAATGTCGAGGAAACAAAAGAAAATGGTGATTCTGTTGTTGAGGCTGTTCAGGAAAAACCAGCTGCAGAATCTGAGACAGAAAATGTTGTTAAGGATGAGAATGTGGTAGCTGAGGCTGAAACTAAGGACAATGTTAAGACAGAAGAAGCCACAGAAGAGAAAAGTGAGGAGAAGGTTGAGAAAGAAGAAGCCTTGGATGAAAAGAAAGAGGAAGAAGTGATCACCAACAATGATGCAAAAATTGAGAAGAGTGAAGAAGAGGCTCCAATTGAGAAGAGTGAATAA
Protein sequence

>Msa0732420

MATVEVVSAQTALSEDTAQLSLKSDEKTTEEIVESPITPAASETKEESSAEEAKPLVKAEAKEVVEEVVVTVKDTAEETKKEEQTETKEPVEETKENGDSVNVEETKENGDSVVEAVQEKPAAESETENVVKDENVVAEAETKDNVKTEEATEEKSEEKVEKEEALDEKKEEEVITNNDAKIEKSEEEAPIEKSE*