AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826310 MtrunA17_Chr5g0447991 88.205 195 18 1 1 190 1 195 5.13e-74 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTAAATAATGCTTATTT+TGG 0.137891 5_3:-66661099 None:intergenic
CTTTGTATGATTATGCATTT+TGG 0.148495 5_3:+66662873 Msa0826310:three_prime_UTR
CAAAGGTTTGGATTCTTCTT+TGG 0.248739 5_3:-66662201 None:intergenic
ACCTCAACAGTGGCCATAAT+TGG 0.274025 5_3:-66661360 None:intergenic
TTCTGTTGTTGAGGCTGTTC+AGG 0.288375 5_3:+66662388 Msa0826310:CDS
TCTGATGCTGCTGGTGTGAT+TGG 0.302513 5_3:-66662176 None:intergenic
GTCGAGGAAACAAAAGAAAA+TGG 0.336894 5_3:+66662362 Msa0826310:CDS
TCTATTGGGAGAATGAATTA+TGG 0.354702 5_3:-66661071 None:intergenic
ATTACTGAAAAGGTTACATT+AGG 0.365107 5_3:-66662844 None:intergenic
GTAAGCATGTTTGGCAATAA+TGG 0.367425 5_3:-66661152 None:intergenic
CTCAACAGGTTCCTTTGTCT+CGG 0.374352 5_3:-66662309 None:intergenic
CTCAGCTTTAACCAAAGGTT+TGG 0.383935 5_3:-66662213 None:intergenic
TCTGTCTCAGATTCTGCAGC+TGG 0.400444 5_3:-66662416 None:intergenic
TGGACTCTCAATGATTTCTT+CGG 0.404920 5_3:-66662156 None:intergenic
TTACATTAGGTAAACTCAAA+TGG 0.406842 5_3:-66662831 None:intergenic
CAAAGAAGAATCCAAACCTT+TGG 0.417637 5_3:+66662202 Msa0826310:CDS
GCTTACTAGCATTCAGTGAA+TGG 0.421591 5_3:+66661169 Msa0826310:five_prime_UTR
CCTTACTCACTCTTCTCAGT+TGG 0.426357 5_3:-66662623 None:intergenic
ATTTAGAATGGTTTATATAG+AGG 0.427250 5_3:-66661258 None:intergenic
TGAGACAGAAAATGTTGTTA+AGG 0.433126 5_3:+66662430 Msa0826310:CDS
TGAATGCTAGTAAGCATGTT+TGG 0.437182 5_3:-66661161 None:intergenic
GGTAGCTGAGGCTGAAACTA+AGG 0.438659 5_3:+66662463 Msa0826310:CDS
ATGTAAATGTCAAATGACTA+AGG 0.440901 5_3:-66662959 None:intergenic
CCAACTGAGAAGAGTGAGTA+AGG 0.442677 5_3:+66662623 Msa0826310:CDS
GGTTGAGAAAGAAGAAGCCT+TGG 0.446199 5_3:+66662532 Msa0826310:CDS
AACAAAGTTAAGATTGCTAT+AGG 0.461132 5_3:-66661315 None:intergenic
TCTTTGGTTTCTGATGCTGC+TGG 0.462673 5_3:-66662185 None:intergenic
CTTACTAGCATTCAGTGAAT+GGG 0.465281 5_3:+66661170 Msa0826310:five_prime_UTR
AAAGGAACATATTACTGAAA+AGG 0.467777 5_3:-66662854 None:intergenic
TACATTAGGTAAACTCAAAT+GGG 0.470364 5_3:-66662830 None:intergenic
TGGTTTATATAGAGGTTTGA+GGG 0.484870 5_3:-66661250 None:intergenic
TCCAATTATGGCCACTGTTG+AGG 0.502667 5_3:+66661359 Msa0826310:exon
AAGTGTTTAATTAGAGAGAG+TGG 0.506459 5_3:+66662668 Msa0826310:three_prime_UTR
TACAGCAGAGGAAACAAAGA+AGG 0.515632 5_3:+66662277 Msa0826310:CDS
AATTGAGAAGAGTGAAGAAG+AGG 0.516526 5_3:+66662598 Msa0826310:CDS
ACTCTCAATGATTTCTTCGG+TGG 0.520132 5_3:-66662153 None:intergenic
GGTTAAAGCTGAGGCAAAAG+AGG 0.520787 5_3:+66662223 Msa0826310:CDS
ATGGTTTATATAGAGGTTTG+AGG 0.531817 5_3:-66661251 None:intergenic
GGAAGAACAAACCGAGACAA+AGG 0.535864 5_3:+66662298 Msa0826310:CDS
AGAAGAGAAAAGTGAGGAGA+AGG 0.537941 5_3:+66662511 Msa0826310:CDS
TTTGCCTCAGCTTTAACCAA+AGG 0.538136 5_3:-66662218 None:intergenic
GTTGAGGAAACAAAAGAAAA+CGG 0.538839 5_3:+66662326 Msa0826310:CDS
TGTAAATGTCAAATGACTAA+GGG 0.557652 5_3:-66662958 None:intergenic
AGCCTTGGATGAAAAGAAAG+AGG 0.562181 5_3:+66662547 Msa0826310:CDS
TGATGAAAGAAATGAGGTGG+GGG 0.570922 5_3:-66661220 None:intergenic
CGAGACAAAGGAACCTGTTG+AGG 0.574462 5_3:+66662310 Msa0826310:CDS
CAAACCTTTGGTTAAAGCTG+AGG 0.576740 5_3:+66662214 Msa0826310:CDS
ATTTGACATTTACATAAACT+CGG 0.580684 5_3:+66662967 Msa0826310:three_prime_UTR
AGTTGATGAAAGAAATGAGG+TGG 0.608604 5_3:-66661223 None:intergenic
AGCTTAGAGATGAGGTAACA+AGG 0.612100 5_3:-66661122 None:intergenic
AAATGGTGATTCTGTTGTTG+AGG 0.613322 5_3:+66662379 Msa0826310:CDS
GTGGCAAAAGCTTAGAGATG+AGG 0.617154 5_3:-66661130 None:intergenic
ACTACTAGTAGCTAAATGCA+TGG 0.617396 5_3:+66662790 Msa0826310:three_prime_UTR
CTACTAGTAGCTAAATGCAT+GGG 0.617641 5_3:+66662791 Msa0826310:three_prime_UTR
TGTTGTTAAGGATGAGAATG+TGG 0.621253 5_3:+66662442 Msa0826310:CDS
GTTGATGAAAGAAATGAGGT+GGG 0.621816 5_3:-66661222 None:intergenic
TGATAACTGAGCAGTGTCGT+CGG 0.622323 5_3:-66662111 None:intergenic
CGGTGATTCAGTTAATGTCG+AGG 0.622446 5_3:+66662346 Msa0826310:CDS
ACAAGTTGATGAAAGAAATG+AGG 0.623867 5_3:-66661226 None:intergenic
CAAAATGCATAATCATACAA+AGG 0.624630 5_3:-66662872 None:intergenic
AGCCACAGAAGAGAAAAGTG+AGG 0.638283 5_3:+66662505 Msa0826310:CDS
AGCATGTTTGGCAATAATGG+TGG 0.642031 5_3:-66661149 None:intergenic
CAACTGAGAAGAGTGAGTAA+GGG 0.654993 5_3:+66662624 Msa0826310:CDS
TTACTAGCATTCAGTGAATG+GGG 0.664936 5_3:+66661171 Msa0826310:five_prime_UTR
GGATGAGAATGTGGTAGCTG+AGG 0.670593 5_3:+66662451 Msa0826310:CDS
GGTGGTAGAAGAAGTTCTTG+TGG 0.675484 5_3:+66662244 Msa0826310:CDS
TAAAGCTGAGGCAAAAGAGG+TGG 0.676484 5_3:+66662226 Msa0826310:CDS
TTGATGAAAGAAATGAGGTG+GGG 0.683097 5_3:-66661221 None:intergenic
GGCTGTGAAAGATACAGCAG+AGG 0.684792 5_3:+66662265 Msa0826310:CDS
TGGTAGAGTTACCTCAACAG+TGG 0.712754 5_3:-66661370 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAATAATGCTTATTT+TGG - chr5_3:66661102-66661121 None:intergenic 10.0%
!!! TTAATTTTGATGAAAAATAA+TGG - chr5_3:66661285-66661304 None:intergenic 10.0%
!! ATGGAAATATTAATTATAAT+TGG + chr5_3:66661747-66661766 Msa0826310:intron 10.0%
!!! TTATTAAATAATGCTTATTT+TGG - chr5_3:66661102-66661121 None:intergenic 10.0%
!!! TTAATTTTGATGAAAAATAA+TGG - chr5_3:66661285-66661304 None:intergenic 10.0%
!! ATGGAAATATTAATTATAAT+TGG + chr5_3:66661747-66661766 Msa0826310:intron 10.0%
!!! AAAAATAATGGAATTTAGAA+TGG - chr5_3:66661273-66661292 None:intergenic 15.0%
!!! AATCAATGTTTAAAATCAAA+TGG - chr5_3:66661442-66661461 None:intergenic 15.0%
!!! AATTTTGAGTCTTTTATTTT+TGG + chr5_3:66661853-66661872 Msa0826310:intron 15.0%
!!! AAAAATAATGGAATTTAGAA+TGG - chr5_3:66661273-66661292 None:intergenic 15.0%
!!! AATCAATGTTTAAAATCAAA+TGG - chr5_3:66661442-66661461 None:intergenic 15.0%
!!! AATTTTGAGTCTTTTATTTT+TGG + chr5_3:66661853-66661872 Msa0826310:intron 15.0%
!!! ATTTAGAATGGTTTATATAG+AGG - chr5_3:66661261-66661280 None:intergenic 20.0%
!!! TTTTCTTTGTATTCCAATTA+TGG + chr5_3:66661347-66661366 Msa0826310:exon 20.0%
!!! TAATATTTTAAAGCATCTAG+TGG + chr5_3:66661475-66661494 Msa0826310:intron 20.0%
!!! AATATTTTAAAGCATCTAGT+GGG + chr5_3:66661476-66661495 Msa0826310:intron 20.0%
!! AATCAAATTGTGATTCATAT+TGG + chr5_3:66661565-66661584 Msa0826310:intron 20.0%
!! AAAAATTGAAGCTCTTAAAA+TGG + chr5_3:66661728-66661747 Msa0826310:intron 20.0%
!! AGATAATTTATCCTTATGAA+CGG - chr5_3:66661891-66661910 None:intergenic 20.0%
!!! TTTACTTTTTAATCGAACTT+TGG - chr5_3:66661922-66661941 None:intergenic 20.0%
!!! GAACAAAATTTGTTTTAGAT+AGG + chr5_3:66661946-66661965 Msa0826310:intron 20.0%
!! ATTTGACATTTACATAAACT+CGG + chr5_3:66662967-66662986 Msa0826310:three_prime_UTR 20.0%
!!! ATTTAGAATGGTTTATATAG+AGG - chr5_3:66661261-66661280 None:intergenic 20.0%
!!! TTTTCTTTGTATTCCAATTA+TGG + chr5_3:66661347-66661366 Msa0826310:exon 20.0%
!!! TAATATTTTAAAGCATCTAG+TGG + chr5_3:66661475-66661494 Msa0826310:intron 20.0%
!!! AATATTTTAAAGCATCTAGT+GGG + chr5_3:66661476-66661495 Msa0826310:intron 20.0%
!! AATCAAATTGTGATTCATAT+TGG + chr5_3:66661565-66661584 Msa0826310:intron 20.0%
!! AAAAATTGAAGCTCTTAAAA+TGG + chr5_3:66661728-66661747 Msa0826310:intron 20.0%
!! AGATAATTTATCCTTATGAA+CGG - chr5_3:66661891-66661910 None:intergenic 20.0%
!!! TTTACTTTTTAATCGAACTT+TGG - chr5_3:66661922-66661941 None:intergenic 20.0%
!!! GAACAAAATTTGTTTTAGAT+AGG + chr5_3:66661946-66661965 Msa0826310:intron 20.0%
!! ATTTGACATTTACATAAACT+CGG + chr5_3:66662967-66662986 Msa0826310:three_prime_UTR 20.0%
!!! TTATTTTGGATCACTCTATT+GGG - chr5_3:66661088-66661107 None:intergenic 25.0%
! AACAAAGTTAAGATTGCTAT+AGG - chr5_3:66661318-66661337 None:intergenic 25.0%
! AAAAACCAAGTACTTGAAAT+TGG - chr5_3:66661393-66661412 None:intergenic 25.0%
!!! TCAATATTTTAATGTCTCTC+CGG - chr5_3:66661644-66661663 None:intergenic 25.0%
!!! ACCTTTTTTAATGCATTAGA+TGG + chr5_3:66661666-66661685 Msa0826310:intron 25.0%
! TCATATCTTCTTGAGTAAAT+AGG - chr5_3:66661701-66661720 None:intergenic 25.0%
! TTTCAATACTCTGACTAATA+TGG - chr5_3:66661825-66661844 None:intergenic 25.0%
! GATAATTTATCCTTATGAAC+GGG - chr5_3:66661890-66661909 None:intergenic 25.0%
!! CAAATGTTGTTTATTAGACA+AGG - chr5_3:66662039-66662058 None:intergenic 25.0%
! TACATTAGGTAAACTCAAAT+GGG - chr5_3:66662833-66662852 None:intergenic 25.0%
! TTACATTAGGTAAACTCAAA+TGG - chr5_3:66662834-66662853 None:intergenic 25.0%
! ATTACTGAAAAGGTTACATT+AGG - chr5_3:66662847-66662866 None:intergenic 25.0%
! AAAGGAACATATTACTGAAA+AGG - chr5_3:66662857-66662876 None:intergenic 25.0%
! CAAAATGCATAATCATACAA+AGG - chr5_3:66662875-66662894 None:intergenic 25.0%
!! CTTTGTATGATTATGCATTT+TGG + chr5_3:66662873-66662892 Msa0826310:three_prime_UTR 25.0%
! TGTAAATGTCAAATGACTAA+GGG - chr5_3:66662961-66662980 None:intergenic 25.0%
! ATGTAAATGTCAAATGACTA+AGG - chr5_3:66662962-66662981 None:intergenic 25.0%
!!! TTATTTTGGATCACTCTATT+GGG - chr5_3:66661088-66661107 None:intergenic 25.0%
! AACAAAGTTAAGATTGCTAT+AGG - chr5_3:66661318-66661337 None:intergenic 25.0%
! AAAAACCAAGTACTTGAAAT+TGG - chr5_3:66661393-66661412 None:intergenic 25.0%
!!! TCAATATTTTAATGTCTCTC+CGG - chr5_3:66661644-66661663 None:intergenic 25.0%
!!! ACCTTTTTTAATGCATTAGA+TGG + chr5_3:66661666-66661685 Msa0826310:intron 25.0%
! TCATATCTTCTTGAGTAAAT+AGG - chr5_3:66661701-66661720 None:intergenic 25.0%
! TTTCAATACTCTGACTAATA+TGG - chr5_3:66661825-66661844 None:intergenic 25.0%
! GATAATTTATCCTTATGAAC+GGG - chr5_3:66661890-66661909 None:intergenic 25.0%
!! CAAATGTTGTTTATTAGACA+AGG - chr5_3:66662039-66662058 None:intergenic 25.0%
! TACATTAGGTAAACTCAAAT+GGG - chr5_3:66662833-66662852 None:intergenic 25.0%
! TTACATTAGGTAAACTCAAA+TGG - chr5_3:66662834-66662853 None:intergenic 25.0%
! ATTACTGAAAAGGTTACATT+AGG - chr5_3:66662847-66662866 None:intergenic 25.0%
! AAAGGAACATATTACTGAAA+AGG - chr5_3:66662857-66662876 None:intergenic 25.0%
! CAAAATGCATAATCATACAA+AGG - chr5_3:66662875-66662894 None:intergenic 25.0%
!! CTTTGTATGATTATGCATTT+TGG + chr5_3:66662873-66662892 Msa0826310:three_prime_UTR 25.0%
! TGTAAATGTCAAATGACTAA+GGG - chr5_3:66662961-66662980 None:intergenic 25.0%
! ATGTAAATGTCAAATGACTA+AGG - chr5_3:66662962-66662981 None:intergenic 25.0%
TCTATTGGGAGAATGAATTA+TGG - chr5_3:66661074-66661093 None:intergenic 30.0%
!!! CTTATTTTGGATCACTCTAT+TGG - chr5_3:66661089-66661108 None:intergenic 30.0%
!! ACAAGTTGATGAAAGAAATG+AGG - chr5_3:66661229-66661248 None:intergenic 30.0%
TGGTTTATATAGAGGTTTGA+GGG - chr5_3:66661253-66661272 None:intergenic 30.0%
ATGGTTTATATAGAGGTTTG+AGG - chr5_3:66661254-66661273 None:intergenic 30.0%
!!! CACATGACATGTTTTAATCA+CGG + chr5_3:66661538-66661557 Msa0826310:intron 30.0%
!!! ACATGACATGTTTTAATCAC+GGG + chr5_3:66661539-66661558 Msa0826310:intron 30.0%
GCCATCTAATGCATTAAAAA+AGG - chr5_3:66661670-66661689 None:intergenic 30.0%
ATCTTCTTGAGTAAATAGGA+AGG - chr5_3:66661697-66661716 None:intergenic 30.0%
ATGTGCATGATTAATTCATG+TGG - chr5_3:66661791-66661810 None:intergenic 30.0%
!! TTTTCTTTTGTTTCCTCAAC+AGG - chr5_3:66662326-66662345 None:intergenic 30.0%
GTTGAGGAAACAAAAGAAAA+CGG + chr5_3:66662326-66662345 Msa0826310:CDS 30.0%
TGAGACAGAAAATGTTGTTA+AGG + chr5_3:66662430-66662449 Msa0826310:CDS 30.0%
! CTCAATTTTTGCATCATTGT+TGG - chr5_3:66662585-66662604 None:intergenic 30.0%
AAGTGTTTAATTAGAGAGAG+TGG + chr5_3:66662668-66662687 Msa0826310:three_prime_UTR 30.0%
TCTATTGGGAGAATGAATTA+TGG - chr5_3:66661074-66661093 None:intergenic 30.0%
!!! CTTATTTTGGATCACTCTAT+TGG - chr5_3:66661089-66661108 None:intergenic 30.0%
!! ACAAGTTGATGAAAGAAATG+AGG - chr5_3:66661229-66661248 None:intergenic 30.0%
TGGTTTATATAGAGGTTTGA+GGG - chr5_3:66661253-66661272 None:intergenic 30.0%
ATGGTTTATATAGAGGTTTG+AGG - chr5_3:66661254-66661273 None:intergenic 30.0%
!!! CACATGACATGTTTTAATCA+CGG + chr5_3:66661538-66661557 Msa0826310:intron 30.0%
!!! ACATGACATGTTTTAATCAC+GGG + chr5_3:66661539-66661558 Msa0826310:intron 30.0%
GCCATCTAATGCATTAAAAA+AGG - chr5_3:66661670-66661689 None:intergenic 30.0%
ATCTTCTTGAGTAAATAGGA+AGG - chr5_3:66661697-66661716 None:intergenic 30.0%
ATGTGCATGATTAATTCATG+TGG - chr5_3:66661791-66661810 None:intergenic 30.0%
!! TTTTCTTTTGTTTCCTCAAC+AGG - chr5_3:66662326-66662345 None:intergenic 30.0%
GTTGAGGAAACAAAAGAAAA+CGG + chr5_3:66662326-66662345 Msa0826310:CDS 30.0%
TGAGACAGAAAATGTTGTTA+AGG + chr5_3:66662430-66662449 Msa0826310:CDS 30.0%
! CTCAATTTTTGCATCATTGT+TGG - chr5_3:66662585-66662604 None:intergenic 30.0%
AAGTGTTTAATTAGAGAGAG+TGG + chr5_3:66662668-66662687 Msa0826310:three_prime_UTR 30.0%
GTAAGCATGTTTGGCAATAA+TGG - chr5_3:66661155-66661174 None:intergenic 35.0%
TGAATGCTAGTAAGCATGTT+TGG - chr5_3:66661164-66661183 None:intergenic 35.0%
CTTACTAGCATTCAGTGAAT+GGG + chr5_3:66661170-66661189 Msa0826310:five_prime_UTR 35.0%
TTACTAGCATTCAGTGAATG+GGG + chr5_3:66661171-66661190 Msa0826310:five_prime_UTR 35.0%
TTGATGAAAGAAATGAGGTG+GGG - chr5_3:66661224-66661243 None:intergenic 35.0%
! GTTGATGAAAGAAATGAGGT+GGG - chr5_3:66661225-66661244 None:intergenic 35.0%
!! AGTTGATGAAAGAAATGAGG+TGG - chr5_3:66661226-66661245 None:intergenic 35.0%
! CTCTACCAATTTCAAGTACT+TGG + chr5_3:66661385-66661404 Msa0826310:intron 35.0%
CGTGATTAAAACATGTCATG+TGG - chr5_3:66661540-66661559 None:intergenic 35.0%
ATTTATCCTTATGAACGGGA+GGG - chr5_3:66661886-66661905 None:intergenic 35.0%
AATTTATCCTTATGAACGGG+AGG - chr5_3:66661887-66661906 None:intergenic 35.0%
TGATCTGAATATGATTCTGC+AGG + chr5_3:66662064-66662083 Msa0826310:intron 35.0%
TGGACTCTCAATGATTTCTT+CGG - chr5_3:66662159-66662178 None:intergenic 35.0%
CAAAGGTTTGGATTCTTCTT+TGG - chr5_3:66662204-66662223 None:intergenic 35.0%
CAAAGAAGAATCCAAACCTT+TGG + chr5_3:66662202-66662221 Msa0826310:CDS 35.0%
GTCGAGGAAACAAAAGAAAA+TGG + chr5_3:66662362-66662381 Msa0826310:CDS 35.0%
AAATGGTGATTCTGTTGTTG+AGG + chr5_3:66662379-66662398 Msa0826310:CDS 35.0%
TGTTGTTAAGGATGAGAATG+TGG + chr5_3:66662442-66662461 Msa0826310:CDS 35.0%
! TTCCTCTTTCTTTTCATCCA+AGG - chr5_3:66662552-66662571 None:intergenic 35.0%
AATTGAGAAGAGTGAAGAAG+AGG + chr5_3:66662598-66662617 Msa0826310:CDS 35.0%
ACTACTAGTAGCTAAATGCA+TGG + chr5_3:66662790-66662809 Msa0826310:three_prime_UTR 35.0%
CTACTAGTAGCTAAATGCAT+GGG + chr5_3:66662791-66662810 Msa0826310:three_prime_UTR 35.0%
GTAAGCATGTTTGGCAATAA+TGG - chr5_3:66661155-66661174 None:intergenic 35.0%
TGAATGCTAGTAAGCATGTT+TGG - chr5_3:66661164-66661183 None:intergenic 35.0%
CTTACTAGCATTCAGTGAAT+GGG + chr5_3:66661170-66661189 Msa0826310:five_prime_UTR 35.0%
TTACTAGCATTCAGTGAATG+GGG + chr5_3:66661171-66661190 Msa0826310:five_prime_UTR 35.0%
TTGATGAAAGAAATGAGGTG+GGG - chr5_3:66661224-66661243 None:intergenic 35.0%
! GTTGATGAAAGAAATGAGGT+GGG - chr5_3:66661225-66661244 None:intergenic 35.0%
!! AGTTGATGAAAGAAATGAGG+TGG - chr5_3:66661226-66661245 None:intergenic 35.0%
! CTCTACCAATTTCAAGTACT+TGG + chr5_3:66661385-66661404 Msa0826310:intron 35.0%
CGTGATTAAAACATGTCATG+TGG - chr5_3:66661540-66661559 None:intergenic 35.0%
ATTTATCCTTATGAACGGGA+GGG - chr5_3:66661886-66661905 None:intergenic 35.0%
AATTTATCCTTATGAACGGG+AGG - chr5_3:66661887-66661906 None:intergenic 35.0%
TGATCTGAATATGATTCTGC+AGG + chr5_3:66662064-66662083 Msa0826310:intron 35.0%
TGGACTCTCAATGATTTCTT+CGG - chr5_3:66662159-66662178 None:intergenic 35.0%
CAAAGGTTTGGATTCTTCTT+TGG - chr5_3:66662204-66662223 None:intergenic 35.0%
CAAAGAAGAATCCAAACCTT+TGG + chr5_3:66662202-66662221 Msa0826310:CDS 35.0%
GTCGAGGAAACAAAAGAAAA+TGG + chr5_3:66662362-66662381 Msa0826310:CDS 35.0%
AAATGGTGATTCTGTTGTTG+AGG + chr5_3:66662379-66662398 Msa0826310:CDS 35.0%
TGTTGTTAAGGATGAGAATG+TGG + chr5_3:66662442-66662461 Msa0826310:CDS 35.0%
! TTCCTCTTTCTTTTCATCCA+AGG - chr5_3:66662552-66662571 None:intergenic 35.0%
AATTGAGAAGAGTGAAGAAG+AGG + chr5_3:66662598-66662617 Msa0826310:CDS 35.0%
ACTACTAGTAGCTAAATGCA+TGG + chr5_3:66662790-66662809 Msa0826310:three_prime_UTR 35.0%
CTACTAGTAGCTAAATGCAT+GGG + chr5_3:66662791-66662810 Msa0826310:three_prime_UTR 35.0%
AGCTTAGAGATGAGGTAACA+AGG - chr5_3:66661125-66661144 None:intergenic 40.0%
AGCATGTTTGGCAATAATGG+TGG - chr5_3:66661152-66661171 None:intergenic 40.0%
GCTTACTAGCATTCAGTGAA+TGG + chr5_3:66661169-66661188 Msa0826310:five_prime_UTR 40.0%
TGATGAAAGAAATGAGGTGG+GGG - chr5_3:66661223-66661242 None:intergenic 40.0%
TGTCATGTGGAAGTAATGCA+GGG - chr5_3:66661527-66661546 None:intergenic 40.0%
ATGTCATGTGGAAGTAATGC+AGG - chr5_3:66661528-66661547 None:intergenic 40.0%
CTCTGACTAATATGGACACA+TGG - chr5_3:66661817-66661836 None:intergenic 40.0%
ACTCTCAATGATTTCTTCGG+TGG - chr5_3:66662156-66662175 None:intergenic 40.0%
! CTCAGCTTTAACCAAAGGTT+TGG - chr5_3:66662216-66662235 None:intergenic 40.0%
CAAACCTTTGGTTAAAGCTG+AGG + chr5_3:66662214-66662233 Msa0826310:CDS 40.0%
! TTTGCCTCAGCTTTAACCAA+AGG - chr5_3:66662221-66662240 None:intergenic 40.0%
TACAGCAGAGGAAACAAAGA+AGG + chr5_3:66662277-66662296 Msa0826310:CDS 40.0%
AGAAGAGAAAAGTGAGGAGA+AGG + chr5_3:66662511-66662530 Msa0826310:CDS 40.0%
AGCCTTGGATGAAAAGAAAG+AGG + chr5_3:66662547-66662566 Msa0826310:CDS 40.0%
CAACTGAGAAGAGTGAGTAA+GGG + chr5_3:66662624-66662643 Msa0826310:CDS 40.0%
AGCTTAGAGATGAGGTAACA+AGG - chr5_3:66661125-66661144 None:intergenic 40.0%
AGCATGTTTGGCAATAATGG+TGG - chr5_3:66661152-66661171 None:intergenic 40.0%
GCTTACTAGCATTCAGTGAA+TGG + chr5_3:66661169-66661188 Msa0826310:five_prime_UTR 40.0%
TGATGAAAGAAATGAGGTGG+GGG - chr5_3:66661223-66661242 None:intergenic 40.0%
TGTCATGTGGAAGTAATGCA+GGG - chr5_3:66661527-66661546 None:intergenic 40.0%
ATGTCATGTGGAAGTAATGC+AGG - chr5_3:66661528-66661547 None:intergenic 40.0%
CTCTGACTAATATGGACACA+TGG - chr5_3:66661817-66661836 None:intergenic 40.0%
ACTCTCAATGATTTCTTCGG+TGG - chr5_3:66662156-66662175 None:intergenic 40.0%
! CTCAGCTTTAACCAAAGGTT+TGG - chr5_3:66662216-66662235 None:intergenic 40.0%
CAAACCTTTGGTTAAAGCTG+AGG + chr5_3:66662214-66662233 Msa0826310:CDS 40.0%
! TTTGCCTCAGCTTTAACCAA+AGG - chr5_3:66662221-66662240 None:intergenic 40.0%
TACAGCAGAGGAAACAAAGA+AGG + chr5_3:66662277-66662296 Msa0826310:CDS 40.0%
AGAAGAGAAAAGTGAGGAGA+AGG + chr5_3:66662511-66662530 Msa0826310:CDS 40.0%
AGCCTTGGATGAAAAGAAAG+AGG + chr5_3:66662547-66662566 Msa0826310:CDS 40.0%
CAACTGAGAAGAGTGAGTAA+GGG + chr5_3:66662624-66662643 Msa0826310:CDS 40.0%
GTGGCAAAAGCTTAGAGATG+AGG - chr5_3:66661133-66661152 None:intergenic 45.0%
TCCAATTATGGCCACTGTTG+AGG + chr5_3:66661359-66661378 Msa0826310:exon 45.0%
ACCTCAACAGTGGCCATAAT+TGG - chr5_3:66661363-66661382 None:intergenic 45.0%
TGGTAGAGTTACCTCAACAG+TGG - chr5_3:66661373-66661392 None:intergenic 45.0%
AAATCGCGTCACGTTGTAAC+CGG + chr5_3:66661622-66661641 Msa0826310:intron 45.0%
TGATAACTGAGCAGTGTCGT+CGG - chr5_3:66662114-66662133 None:intergenic 45.0%
! TCTTTGGTTTCTGATGCTGC+TGG - chr5_3:66662188-66662207 None:intergenic 45.0%
GGTTAAAGCTGAGGCAAAAG+AGG + chr5_3:66662223-66662242 Msa0826310:CDS 45.0%
TAAAGCTGAGGCAAAAGAGG+TGG + chr5_3:66662226-66662245 Msa0826310:CDS 45.0%
GGTGGTAGAAGAAGTTCTTG+TGG + chr5_3:66662244-66662263 Msa0826310:CDS 45.0%
GGAAGAACAAACCGAGACAA+AGG + chr5_3:66662298-66662317 Msa0826310:CDS 45.0%
CTCAACAGGTTCCTTTGTCT+CGG - chr5_3:66662312-66662331 None:intergenic 45.0%
CGGTGATTCAGTTAATGTCG+AGG + chr5_3:66662346-66662365 Msa0826310:CDS 45.0%
TTCTGTTGTTGAGGCTGTTC+AGG + chr5_3:66662388-66662407 Msa0826310:CDS 45.0%
AGCCACAGAAGAGAAAAGTG+AGG + chr5_3:66662505-66662524 Msa0826310:CDS 45.0%
!! CTCCTCACTTTTCTCTTCTG+TGG - chr5_3:66662510-66662529 None:intergenic 45.0%
GGTTGAGAAAGAAGAAGCCT+TGG + chr5_3:66662532-66662551 Msa0826310:CDS 45.0%
CCTTACTCACTCTTCTCAGT+TGG - chr5_3:66662626-66662645 None:intergenic 45.0%
CCAACTGAGAAGAGTGAGTA+AGG + chr5_3:66662623-66662642 Msa0826310:CDS 45.0%
GTGGCAAAAGCTTAGAGATG+AGG - chr5_3:66661133-66661152 None:intergenic 45.0%
TCCAATTATGGCCACTGTTG+AGG + chr5_3:66661359-66661378 Msa0826310:exon 45.0%
ACCTCAACAGTGGCCATAAT+TGG - chr5_3:66661363-66661382 None:intergenic 45.0%
TGGTAGAGTTACCTCAACAG+TGG - chr5_3:66661373-66661392 None:intergenic 45.0%
AAATCGCGTCACGTTGTAAC+CGG + chr5_3:66661622-66661641 Msa0826310:intron 45.0%
TGATAACTGAGCAGTGTCGT+CGG - chr5_3:66662114-66662133 None:intergenic 45.0%
! TCTTTGGTTTCTGATGCTGC+TGG - chr5_3:66662188-66662207 None:intergenic 45.0%
GGTTAAAGCTGAGGCAAAAG+AGG + chr5_3:66662223-66662242 Msa0826310:CDS 45.0%
TAAAGCTGAGGCAAAAGAGG+TGG + chr5_3:66662226-66662245 Msa0826310:CDS 45.0%
GGTGGTAGAAGAAGTTCTTG+TGG + chr5_3:66662244-66662263 Msa0826310:CDS 45.0%
GGAAGAACAAACCGAGACAA+AGG + chr5_3:66662298-66662317 Msa0826310:CDS 45.0%
CTCAACAGGTTCCTTTGTCT+CGG - chr5_3:66662312-66662331 None:intergenic 45.0%
CGGTGATTCAGTTAATGTCG+AGG + chr5_3:66662346-66662365 Msa0826310:CDS 45.0%
TTCTGTTGTTGAGGCTGTTC+AGG + chr5_3:66662388-66662407 Msa0826310:CDS 45.0%
AGCCACAGAAGAGAAAAGTG+AGG + chr5_3:66662505-66662524 Msa0826310:CDS 45.0%
!! CTCCTCACTTTTCTCTTCTG+TGG - chr5_3:66662510-66662529 None:intergenic 45.0%
GGTTGAGAAAGAAGAAGCCT+TGG + chr5_3:66662532-66662551 Msa0826310:CDS 45.0%
CCTTACTCACTCTTCTCAGT+TGG - chr5_3:66662626-66662645 None:intergenic 45.0%
CCAACTGAGAAGAGTGAGTA+AGG + chr5_3:66662623-66662642 Msa0826310:CDS 45.0%
GGGGGCAGTTCAAGAATCTT+GGG - chr5_3:66661205-66661224 None:intergenic 50.0%
TGGGGGCAGTTCAAGAATCT+TGG - chr5_3:66661206-66661225 None:intergenic 50.0%
CTCAAACCCTCCCGTTCATA+AGG + chr5_3:66661877-66661896 Msa0826310:intron 50.0%
TCTGATGCTGCTGGTGTGAT+TGG - chr5_3:66662179-66662198 None:intergenic 50.0%
GGCTGTGAAAGATACAGCAG+AGG + chr5_3:66662265-66662284 Msa0826310:CDS 50.0%
CGAGACAAAGGAACCTGTTG+AGG + chr5_3:66662310-66662329 Msa0826310:CDS 50.0%
TCTGTCTCAGATTCTGCAGC+TGG - chr5_3:66662419-66662438 None:intergenic 50.0%
GGATGAGAATGTGGTAGCTG+AGG + chr5_3:66662451-66662470 Msa0826310:CDS 50.0%
GGTAGCTGAGGCTGAAACTA+AGG + chr5_3:66662463-66662482 Msa0826310:CDS 50.0%
GGGGGCAGTTCAAGAATCTT+GGG - chr5_3:66661205-66661224 None:intergenic 50.0%
TGGGGGCAGTTCAAGAATCT+TGG - chr5_3:66661206-66661225 None:intergenic 50.0%
CTCAAACCCTCCCGTTCATA+AGG + chr5_3:66661877-66661896 Msa0826310:intron 50.0%
TCTGATGCTGCTGGTGTGAT+TGG - chr5_3:66662179-66662198 None:intergenic 50.0%
GGCTGTGAAAGATACAGCAG+AGG + chr5_3:66662265-66662284 Msa0826310:CDS 50.0%
CGAGACAAAGGAACCTGTTG+AGG + chr5_3:66662310-66662329 Msa0826310:CDS 50.0%
TCTGTCTCAGATTCTGCAGC+TGG - chr5_3:66662419-66662438 None:intergenic 50.0%
GGATGAGAATGTGGTAGCTG+AGG + chr5_3:66662451-66662470 Msa0826310:CDS 50.0%
GGTAGCTGAGGCTGAAACTA+AGG + chr5_3:66662463-66662482 Msa0826310:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 66661058 66662996 66661058 ID=Msa0826310;Name=Msa0826310
chr5_3 mRNA 66661058 66662996 66661058 ID=Msa0826310-mRNA-1;Parent=Msa0826310;Name=Msa0826310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=308|1|1|1|1|1|2|353|190
chr5_3 exon 66661058 66661380 66661058 ID=Msa0826310-mRNA-1:exon:20905;Parent=Msa0826310-mRNA-1
chr5_3 exon 66662086 66662996 66662086 ID=Msa0826310-mRNA-1:exon:20906;Parent=Msa0826310-mRNA-1
chr5_3 five_prime_UTR 66661058 66661365 66661058 ID=Msa0826310-mRNA-1:five_prime_utr;Parent=Msa0826310-mRNA-1
chr5_3 CDS 66661366 66661380 66661366 ID=Msa0826310-mRNA-1:cds;Parent=Msa0826310-mRNA-1
chr5_3 CDS 66662086 66662643 66662086 ID=Msa0826310-mRNA-1:cds;Parent=Msa0826310-mRNA-1
chr5_3 three_prime_UTR 66662644 66662996 66662644 ID=Msa0826310-mRNA-1:three_prime_utr;Parent=Msa0826310-mRNA-1
Gene Sequence

>Msa0826310

ATGGCCACTGTTGAGGTTGTATCAGCACAGACTGCACTCTCCGACGACACTGCTCAGTTATCATTGAAATCTGATGAAAAAACCACCGAAGAAATCATTGAGAGTCCAATCACACCAGCAGCATCAGAAACCAAAGAAGAATCCAAACCTTTGGTTAAAGCTGAGGCAAAAGAGGTGGTAGAAGAAGTTCTTGTGGCTGTGAAAGATACAGCAGAGGAAACAAAGAAGGAAGAACAAACCGAGACAAAGGAACCTGTTGAGGAAACAAAAGAAAACGGTGATTCAGTTAATGTCGAGGAAACAAAAGAAAATGGTGATTCTGTTGTTGAGGCTGTTCAGGAAAAACCAGCTGCAGAATCTGAGACAGAAAATGTTGTTAAGGATGAGAATGTGGTAGCTGAGGCTGAAACTAAGGACAATGTTAAGACAGAAGAAGCCACAGAAGAGAAAAGTGAGGAGAAGGTTGAGAAAGAAGAAGCCTTGGATGAAAAGAAAGAGGAAGAAGTGATCACCAACAATGATGCAAAAATTGAGAAGAGTGAAGAAGAGGCTCCAACTGAGAAGAGTGAGTAA
Protein sequence

>Msa0826310

MATVEVVSAQTALSDDTAQLSLKSDEKTTEEIIESPITPAASETKEESKPLVKAEAKEVVEEVLVAVKDTAEETKKEEQTETKEPVEETKENGDSVNVEETKENGDSVVEAVQEKPAAESETENVVKDENVVAEAETKDNVKTEEATEEKSEEKVEKEEALDEKKEEEVITNNDAKIEKSEEEAPTEKSE*