Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0742700 | XP_003617604.1 | 92.188 | 192 | 6 | 2 | 1 | 183 | 3 | 194 | 5.32e-117 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0742700 | G7K8L0 | 92.188 | 192 | 6 | 2 | 1 | 183 | 3 | 194 | 2.54e-117 | 340 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0742700 | Msa0827570 | 0.849374 | 3.369845e-60 | -8.615850e-47 |
| Msa0742700 | Msa0862740 | 0.837156 | 6.139280e-57 | -8.615850e-47 |
| Msa0742700 | Msa1413130 | 0.801309 | 9.570311e-49 | -8.615850e-47 |
| Msa0241750 | Msa0742700 | 0.808274 | 3.356318e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0742700 | MtrunA17_Chr5g0444601 | 92.188 | 192 | 6 | 2 | 1 | 183 | 3 | 194 | 4.89e-121 | 340 |
| Msa0742700 | MtrunA17_Chr5g0444611 | 79.679 | 187 | 28 | 3 | 1 | 178 | 47 | 232 | 7.07e-81 | 240 |
| Msa0742700 | MtrunA17_Chr3g0099551 | 44.444 | 189 | 73 | 6 | 1 | 178 | 1 | 168 | 1.52e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAGATTTATGGTGTTAA+TGG | 0.238978 | 5_1:+62670685 | Msa0742700:CDS |
| CCTTGTTACCTTCCTCTCTT+AGG | 0.249740 | 5_1:+62670291 | Msa0742700:CDS |
| GAAGTAGAAGATGAGATTTA+TGG | 0.261780 | 5_1:+62670676 | Msa0742700:CDS |
| TGTTCTAGCATTTCATGTTT+CGG | 0.336351 | 5_1:-62670433 | None:intergenic |
| AGATTTATTAACTTTGTTCA+TGG | 0.336906 | 5_1:-62670222 | None:intergenic |
| GCAAAAGCTGAAGCTTTCAT+TGG | 0.375645 | 5_1:+62670721 | Msa0742700:CDS |
| GGATGTTGAAGATTAAATAA+AGG | 0.422149 | 5_1:-62670322 | None:intergenic |
| AGATGCAGAGAGAGGGCTGC+TGG | 0.439749 | 5_1:+62670764 | Msa0742700:CDS |
| CCTAAGAGAGGAAGGTAACA+AGG | 0.502209 | 5_1:-62670291 | None:intergenic |
| CAGCTCCAAGTGCAATGATA+AGG | 0.503544 | 5_1:-62670343 | None:intergenic |
| AACATCCTTATCATTGCACT+TGG | 0.507384 | 5_1:+62670338 | Msa0742700:CDS |
| AGAAGATGATTTAGAAGTGA+AGG | 0.515399 | 5_1:+62670642 | Msa0742700:CDS |
| TGATTTAGAAGTGAAGGATG+AGG | 0.517126 | 5_1:+62670648 | Msa0742700:CDS |
| TGGTGTTAGTAAAGTGGATA+AGG | 0.531732 | 5_1:+62670573 | Msa0742700:CDS |
| AAGATTAAATAAAGGTAACT+AGG | 0.532639 | 5_1:-62670314 | None:intergenic |
| GATGCAGAGAGAGGGCTGCT+GGG | 0.533020 | 5_1:+62670765 | Msa0742700:CDS |
| ATTGCACTTGGAGCTGAAGC+CGG | 0.547486 | 5_1:+62670350 | Msa0742700:CDS |
| GTATGTTGGTGTTAGTAAAG+TGG | 0.551450 | 5_1:+62670567 | Msa0742700:CDS |
| TAACTAGGCCTAAGAGAGGA+AGG | 0.551563 | 5_1:-62670299 | None:intergenic |
| ACAAAATGAAAAGAAGCCTA+AGG | 0.566232 | 5_1:+62670510 | Msa0742700:CDS |
| CAGAGAACCCGACAAAAGAC+CGG | 0.573910 | 5_1:-62670369 | None:intergenic |
| CAGTTGAAGATGCAGAGAGA+GGG | 0.576252 | 5_1:+62670757 | Msa0742700:CDS |
| GTAACAAGGGTGTGATCACA+AGG | 0.579955 | 5_1:-62670277 | None:intergenic |
| GCAGTTGAAGATGCAGAGAG+AGG | 0.589013 | 5_1:+62670756 | Msa0742700:CDS |
| GAGAAGAAGATTGTGTATGT+TGG | 0.605699 | 5_1:+62670553 | Msa0742700:CDS |
| AAGGTAACTAGGCCTAAGAG+AGG | 0.633233 | 5_1:-62670303 | None:intergenic |
| CTAAGAGAGGAAGGTAACAA+GGG | 0.639907 | 5_1:-62670290 | None:intergenic |
| AAATGAAAAGAAGCCTAAGG+TGG | 0.649751 | 5_1:+62670513 | Msa0742700:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTCTATATAAAAAATACT+TGG | - | chr5_1:62670619-62670638 | None:intergenic | 15.0% |
| !! | AAGATTAAATAAAGGTAACT+AGG | - | chr5_1:62670317-62670336 | None:intergenic | 20.0% |
| ! | GGATGTTGAAGATTAAATAA+AGG | - | chr5_1:62670325-62670344 | None:intergenic | 25.0% |
| !! | TAAAAAATACTTGGCATTGA+AGG | - | chr5_1:62670610-62670629 | None:intergenic | 25.0% |
| TGTTCTAGCATTTCATGTTT+CGG | - | chr5_1:62670436-62670455 | None:intergenic | 30.0% | |
| ACAAAATGAAAAGAAGCCTA+AGG | + | chr5_1:62670510-62670529 | Msa0742700:CDS | 30.0% | |
| AGAAGATGATTTAGAAGTGA+AGG | + | chr5_1:62670642-62670661 | Msa0742700:CDS | 30.0% | |
| GAAGTAGAAGATGAGATTTA+TGG | + | chr5_1:62670676-62670695 | Msa0742700:CDS | 30.0% | |
| ! | GATGAGATTTATGGTGTTAA+TGG | + | chr5_1:62670685-62670704 | Msa0742700:CDS | 30.0% |
| AACATCCTTATCATTGCACT+TGG | + | chr5_1:62670338-62670357 | Msa0742700:CDS | 35.0% | |
| !!! | TGTTGCTTTTTGTCTTCAGA+AGG | - | chr5_1:62670397-62670416 | None:intergenic | 35.0% |
| AAATGAAAAGAAGCCTAAGG+TGG | + | chr5_1:62670513-62670532 | Msa0742700:CDS | 35.0% | |
| !! | ACAGATTTTTCAACCACCTT+AGG | - | chr5_1:62670529-62670548 | None:intergenic | 35.0% |
| GAGAAGAAGATTGTGTATGT+TGG | + | chr5_1:62670553-62670572 | Msa0742700:CDS | 35.0% | |
| !! | GTATGTTGGTGTTAGTAAAG+TGG | + | chr5_1:62670567-62670586 | Msa0742700:CDS | 35.0% |
| ! | TGGTGTTAGTAAAGTGGATA+AGG | + | chr5_1:62670573-62670592 | Msa0742700:CDS | 35.0% |
| TGATTTAGAAGTGAAGGATG+AGG | + | chr5_1:62670648-62670667 | Msa0742700:CDS | 35.0% | |
| CTAAGAGAGGAAGGTAACAA+GGG | - | chr5_1:62670293-62670312 | None:intergenic | 40.0% | |
| GCAAAAGCTGAAGCTTTCAT+TGG | + | chr5_1:62670721-62670740 | Msa0742700:CDS | 40.0% | |
| ! | GTAACAAGGGTGTGATCACA+AGG | - | chr5_1:62670280-62670299 | None:intergenic | 45.0% |
| CCTAAGAGAGGAAGGTAACA+AGG | - | chr5_1:62670294-62670313 | None:intergenic | 45.0% | |
| CCTTGTTACCTTCCTCTCTT+AGG | + | chr5_1:62670291-62670310 | Msa0742700:CDS | 45.0% | |
| TAACTAGGCCTAAGAGAGGA+AGG | - | chr5_1:62670302-62670321 | None:intergenic | 45.0% | |
| AAGGTAACTAGGCCTAAGAG+AGG | - | chr5_1:62670306-62670325 | None:intergenic | 45.0% | |
| CAGCTCCAAGTGCAATGATA+AGG | - | chr5_1:62670346-62670365 | None:intergenic | 45.0% | |
| CAGTTGAAGATGCAGAGAGA+GGG | + | chr5_1:62670757-62670776 | Msa0742700:CDS | 45.0% | |
| ATTGCACTTGGAGCTGAAGC+CGG | + | chr5_1:62670350-62670369 | Msa0742700:CDS | 50.0% | |
| ! | AGCTGAAGCCGGTCTTTTGT+CGG | + | chr5_1:62670361-62670380 | Msa0742700:CDS | 50.0% |
| CAGAGAACCCGACAAAAGAC+CGG | - | chr5_1:62670372-62670391 | None:intergenic | 50.0% | |
| GCAGTTGAAGATGCAGAGAG+AGG | + | chr5_1:62670756-62670775 | Msa0742700:CDS | 50.0% | |
| ! | GCTGAAGCCGGTCTTTTGTC+GGG | + | chr5_1:62670362-62670381 | Msa0742700:CDS | 55.0% |
| AGATGCAGAGAGAGGGCTGC+TGG | + | chr5_1:62670764-62670783 | Msa0742700:CDS | 60.0% | |
| GATGCAGAGAGAGGGCTGCT+GGG | + | chr5_1:62670765-62670784 | Msa0742700:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 62670224 | 62670795 | 62670224 | ID=Msa0742700;Name=Msa0742700 |
| chr5_1 | mRNA | 62670224 | 62670795 | 62670224 | ID=Msa0742700-mRNA-1;Parent=Msa0742700;Name=Msa0742700-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|0|0.5|2|0|183 |
| chr5_1 | exon | 62670224 | 62670383 | 62670224 | ID=Msa0742700-mRNA-1:exon:18423;Parent=Msa0742700-mRNA-1 |
| chr5_1 | exon | 62670404 | 62670795 | 62670404 | ID=Msa0742700-mRNA-1:exon:18424;Parent=Msa0742700-mRNA-1 |
| chr5_1 | CDS | 62670224 | 62670383 | 62670224 | ID=Msa0742700-mRNA-1:cds;Parent=Msa0742700-mRNA-1 |
| chr5_1 | CDS | 62670404 | 62670795 | 62670404 | ID=Msa0742700-mRNA-1:cds;Parent=Msa0742700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
