Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0827570 | G7K8L0 | 95.312 | 192 | 7 | 1 | 1 | 190 | 3 | 194 | 3.39e-124 | 357 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002110 | Msa0827570 | -0.823153 | 1.614361e-53 | -8.615850e-47 |
| Msa0007810 | Msa0827570 | -0.815904 | 7.280343e-52 | -8.615850e-47 |
| Msa0011060 | Msa0827570 | -0.808583 | 2.884332e-50 | -8.615850e-47 |
| Msa0011440 | Msa0827570 | -0.803329 | 3.672101e-49 | -8.615850e-47 |
| Msa0013270 | Msa0827570 | 0.809895 | 1.509211e-50 | -8.615850e-47 |
| Msa0017300 | Msa0827570 | -0.825719 | 4.020414e-54 | -8.615850e-47 |
| Msa0025570 | Msa0827570 | 0.803261 | 3.793521e-49 | -8.615850e-47 |
| Msa0044530 | Msa0827570 | -0.813366 | 2.654754e-51 | -8.615850e-47 |
| Msa0056230 | Msa0827570 | -0.818140 | 2.289272e-52 | -8.615850e-47 |
| Msa0062190 | Msa0827570 | 0.823734 | 1.180719e-53 | -8.615850e-47 |
| Msa0065670 | Msa0827570 | -0.810925 | 9.042841e-51 | -8.615850e-47 |
| Msa0073110 | Msa0827570 | 0.818092 | 2.347958e-52 | -8.615850e-47 |
| Msa0075990 | Msa0827570 | -0.801682 | 8.025773e-49 | -8.615850e-47 |
| Msa0077570 | Msa0827570 | -0.830250 | 3.264408e-55 | -8.615850e-47 |
| Msa0091240 | Msa0827570 | -0.804460 | 2.137614e-49 | -8.615850e-47 |
| Msa0095810 | Msa0827570 | -0.823852 | 1.107999e-53 | -8.615850e-47 |
| Msa0106540 | Msa0827570 | 0.827225 | 1.759444e-54 | -8.615850e-47 |
| Msa0111110 | Msa0827570 | -0.804224 | 2.393809e-49 | -8.615850e-47 |
| Msa0120160 | Msa0827570 | -0.800544 | 1.371539e-48 | -8.615850e-47 |
| Msa0615070 | Msa0827570 | 0.811559 | 6.589014e-51 | -8.615850e-47 |
| Msa0645390 | Msa0827570 | 0.811742 | 6.014199e-51 | -8.615850e-47 |
| Msa0676570 | Msa0827570 | -0.808147 | 3.573381e-50 | -8.615850e-47 |
| Msa0742370 | Msa0827570 | -0.806206 | 9.206625e-50 | -8.615850e-47 |
| Msa0742700 | Msa0827570 | 0.849374 | 3.369845e-60 | -8.615850e-47 |
| Msa0745140 | Msa0827570 | 0.817544 | 3.121413e-52 | -8.615850e-47 |
| Msa0769910 | Msa0827570 | -0.825280 | 5.110036e-54 | -8.615850e-47 |
| Msa0770540 | Msa0827570 | -0.801063 | 1.074198e-48 | -8.615850e-47 |
| Msa0776640 | Msa0827570 | 0.816667 | 4.913428e-52 | -8.615850e-47 |
| Msa0790650 | Msa0827570 | -0.804579 | 2.018622e-49 | -8.615850e-47 |
| Msa0792410 | Msa0827570 | 0.807772 | 4.293746e-50 | -8.615850e-47 |
| Msa0795910 | Msa0827570 | -0.806877 | 6.643533e-50 | -8.615850e-47 |
| Msa0800120 | Msa0827570 | 0.800018 | 1.754983e-48 | -8.615850e-47 |
| Msa0823480 | Msa0827570 | -0.801669 | 8.072352e-49 | -8.615850e-47 |
| Msa0825920 | Msa0827570 | 0.812252 | 4.654298e-51 | -8.615850e-47 |
| Msa0827570 | Msa0829250 | -0.807481 | 4.950673e-50 | -8.615850e-47 |
| Msa0827570 | Msa0833360 | -0.805919 | 1.058008e-49 | -8.615850e-47 |
| Msa0827570 | Msa0862480 | -0.805822 | 1.108661e-49 | -8.615850e-47 |
| Msa0827570 | Msa0862740 | 0.974618 | 1.784400e-138 | -8.615850e-47 |
| Msa0827570 | Msa0865420 | 0.808271 | 3.362080e-50 | -8.615850e-47 |
| Msa0827570 | Msa0903590 | -0.820149 | 7.988003e-53 | -8.615850e-47 |
| Msa0827570 | Msa0931680 | 0.805849 | 1.094174e-49 | -8.615850e-47 |
| Msa0827570 | Msa0938150 | -0.802664 | 5.040160e-49 | -8.615850e-47 |
| Msa0827570 | Msa0964400 | -0.810564 | 1.082432e-50 | -8.615850e-47 |
| Msa0827570 | Msa1029820 | 0.807418 | 5.103641e-50 | -8.615850e-47 |
| Msa0827570 | Msa1039440 | -0.814457 | 1.525966e-51 | -8.615850e-47 |
| Msa0827570 | Msa1041060 | 0.808757 | 2.647825e-50 | -8.615850e-47 |
| Msa0827570 | Msa1044250 | 0.813534 | 2.437568e-51 | -8.615850e-47 |
| Msa0827570 | Msa1068940 | 0.803659 | 3.137474e-49 | -8.615850e-47 |
| Msa0827570 | Msa1078590 | -0.816599 | 5.088668e-52 | -8.615850e-47 |
| Msa0827570 | Msa1078760 | -0.821342 | 4.248663e-53 | -8.615850e-47 |
| Msa0827570 | Msa1088400 | -0.807054 | 6.096897e-50 | -8.615850e-47 |
| Msa0827570 | Msa1088740 | -0.803782 | 2.957447e-49 | -8.615850e-47 |
| Msa0827570 | Msa1106730 | -0.802753 | 4.831667e-49 | -8.615850e-47 |
| Msa0827570 | Msa1111910 | 0.813684 | 2.260053e-51 | -8.615850e-47 |
| Msa0827570 | Msa1129120 | -0.811172 | 7.998006e-51 | -8.615850e-47 |
| Msa0827570 | Msa1132880 | -0.808162 | 3.546483e-50 | -8.615850e-47 |
| Msa0827570 | Msa1149910 | 0.826648 | 2.417987e-54 | -8.615850e-47 |
| Msa0827570 | Msa1154320 | 0.809582 | 1.761764e-50 | -8.615850e-47 |
| Msa0827570 | Msa1165460 | 0.810812 | 9.568481e-51 | -8.615850e-47 |
| Msa0827570 | Msa1169590 | -0.808705 | 2.716163e-50 | -8.615850e-47 |
| Msa0827570 | Msa1190350 | -0.803195 | 3.914525e-49 | -8.615850e-47 |
| Msa0827570 | Msa1266120 | -0.814729 | 1.328372e-51 | -8.615850e-47 |
| Msa0827570 | Msa1268360 | 0.808001 | 3.838568e-50 | -8.615850e-47 |
| Msa0827570 | Msa1273320 | 0.811858 | 5.673031e-51 | -8.615850e-47 |
| Msa0827570 | Msa1286860 | -0.800315 | 1.527000e-48 | -8.615850e-47 |
| Msa0827570 | Msa1295210 | -0.811400 | 7.136640e-51 | -8.615850e-47 |
| Msa0827570 | Msa1295480 | -0.821413 | 4.091522e-53 | -8.615850e-47 |
| Msa0827570 | Msa1342360 | -0.804710 | 1.896003e-49 | -8.615850e-47 |
| Msa0827570 | Msa1352600 | -0.810627 | 1.049265e-50 | -8.615850e-47 |
| Msa0827570 | Msa1366140 | -0.818633 | 1.770553e-52 | -8.615850e-47 |
| Msa0827570 | Msa1368390 | 0.809107 | 2.228516e-50 | -8.615850e-47 |
| Msa0827570 | Msa1387450 | -0.825320 | 4.998216e-54 | -8.615850e-47 |
| Msa0827570 | Msa1388720 | -0.810841 | 9.433219e-51 | -8.615850e-47 |
| Msa0827570 | Msa1405830 | 0.820619 | 6.231936e-53 | -8.615850e-47 |
| Msa0827570 | Msa1407810 | 0.813394 | 2.617775e-51 | -8.615850e-47 |
| Msa0827570 | Msa1422860 | 0.827426 | 1.574551e-54 | -8.615850e-47 |
| Msa0827570 | Msa1428230 | -0.823536 | 1.313767e-53 | -8.615850e-47 |
| Msa0827570 | Msa1433360 | 0.813521 | 2.453765e-51 | -8.615850e-47 |
| Msa0827570 | Msa1437780 | 0.813962 | 1.962242e-51 | -8.615850e-47 |
| Msa0827570 | Msa1457770 | 0.802064 | 6.698225e-49 | -8.615850e-47 |
| Msa0827570 | Msa1462610 | 0.805384 | 1.370079e-49 | -8.615850e-47 |
| Msa0121320 | Msa0827570 | 0.800675 | 1.289229e-48 | -8.615850e-47 |
| Msa0136180 | Msa0827570 | -0.820192 | 7.810753e-53 | -8.615850e-47 |
| Msa0141410 | Msa0827570 | 0.812318 | 4.503269e-51 | -8.615850e-47 |
| Msa0151510 | Msa0827570 | -0.808539 | 2.946786e-50 | -8.615850e-47 |
| Msa0173600 | Msa0827570 | -0.802465 | 5.538695e-49 | -8.615850e-47 |
| Msa0183310 | Msa0827570 | -0.815310 | 9.868672e-52 | -8.615850e-47 |
| Msa0198990 | Msa0827570 | 0.821013 | 5.060171e-53 | -8.615850e-47 |
| Msa0211460 | Msa0827570 | 0.815711 | 8.037831e-52 | -8.615850e-47 |
| Msa0224870 | Msa0827570 | 0.814689 | 1.355411e-51 | -8.615850e-47 |
| Msa0225790 | Msa0827570 | -0.815161 | 1.065363e-51 | -8.615850e-47 |
| Msa0234150 | Msa0827570 | 0.808413 | 3.135577e-50 | -8.615850e-47 |
| Msa0364910 | Msa0827570 | -0.808774 | 2.625576e-50 | -8.615850e-47 |
| Msa0371350 | Msa0827570 | -0.819752 | 9.846414e-53 | -8.615850e-47 |
| Msa0386880 | Msa0827570 | 0.812719 | 3.679881e-51 | -8.615850e-47 |
| Msa0403740 | Msa0827570 | -0.809503 | 1.832447e-50 | -8.615850e-47 |
| Msa0412580 | Msa0827570 | -0.828672 | 7.892255e-55 | -8.615850e-47 |
| Msa0438680 | Msa0827570 | -0.801814 | 7.537496e-49 | -8.615850e-47 |
| Msa0450890 | Msa0827570 | -0.804340 | 2.263789e-49 | -8.615850e-47 |
| Msa0456450 | Msa0827570 | -0.805100 | 1.570879e-49 | -8.615850e-47 |
| Msa0457070 | Msa0827570 | -0.813870 | 2.055860e-51 | -8.615850e-47 |
| Msa0241750 | Msa0827570 | 0.838949 | 2.121907e-57 | -8.615850e-47 |
| Msa0253230 | Msa0827570 | -0.805025 | 1.628769e-49 | -8.615850e-47 |
| Msa0260640 | Msa0827570 | -0.806744 | 7.090603e-50 | -8.615850e-47 |
| Msa0261350 | Msa0827570 | -0.804314 | 2.292414e-49 | -8.615850e-47 |
| Msa0266140 | Msa0827570 | -0.809619 | 1.730026e-50 | -8.615850e-47 |
| Msa0267450 | Msa0827570 | -0.814143 | 1.790289e-51 | -8.615850e-47 |
| Msa0267510 | Msa0827570 | -0.819849 | 9.358045e-53 | -8.615850e-47 |
| Msa0269220 | Msa0827570 | -0.819113 | 1.377574e-52 | -8.615850e-47 |
| Msa0279810 | Msa0827570 | 0.827374 | 1.620684e-54 | -8.615850e-47 |
| Msa0287480 | Msa0827570 | 0.811369 | 7.247764e-51 | -8.615850e-47 |
| Msa0299130 | Msa0827570 | -0.803896 | 2.800346e-49 | -8.615850e-47 |
| Msa0307630 | Msa0827570 | 0.809658 | 1.697446e-50 | -8.615850e-47 |
| Msa0319220 | Msa0827570 | 0.825326 | 4.983263e-54 | -8.615850e-47 |
| Msa0326170 | Msa0827570 | -0.809383 | 1.944719e-50 | -8.615850e-47 |
| Msa0331070 | Msa0827570 | -0.804857 | 1.766193e-49 | -8.615850e-47 |
| Msa0340700 | Msa0827570 | -0.813104 | 3.030352e-51 | -8.615850e-47 |
| Msa0364030 | Msa0827570 | -0.809664 | 1.692450e-50 | -8.615850e-47 |
| Msa0521610 | Msa0827570 | 0.800043 | 1.734493e-48 | -8.615850e-47 |
| Msa0567330 | Msa0827570 | 0.818731 | 1.682161e-52 | -8.615850e-47 |
| Msa0567570 | Msa0827570 | 0.806009 | 1.013036e-49 | -8.615850e-47 |
| Msa0576320 | Msa0827570 | 0.811686 | 6.182946e-51 | -8.615850e-47 |
| Msa0580410 | Msa0827570 | -0.806604 | 7.590044e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0827570 | MtrunA17_Chr5g0444601 | 95.312 | 192 | 7 | 1 | 1 | 190 | 3 | 194 | 6.52e-128 | 357 |
| Msa0827570 | MtrunA17_Chr5g0444611 | 83.422 | 187 | 28 | 2 | 1 | 185 | 47 | 232 | 8.90e-89 | 260 |
| Msa0827570 | MtrunA17_Chr3g0099551 | 45.503 | 189 | 78 | 5 | 1 | 185 | 1 | 168 | 3.43e-33 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAGATTTATGGTGTTAA+TGG | 0.238978 | 5_3:+68939633 | Msa0827570:CDS |
| CCTTGTTACCTTCCTCTCTT+AGG | 0.249740 | 5_3:+68939238 | Msa0827570:CDS |
| GAAGTAGAAGATGAGATTTA+TGG | 0.261780 | 5_3:+68939624 | Msa0827570:CDS |
| TGTTCTAGCATTTCATGTTT+CGG | 0.336351 | 5_3:-68939381 | None:intergenic |
| AGATTTATTAACTTTGTTCA+TGG | 0.336906 | 5_3:-68939169 | None:intergenic |
| GCAAAAGCTGAAGCTTTCAT+TGG | 0.375645 | 5_3:+68939669 | Msa0827570:CDS |
| GAATGTTGAAGATTAAATAA+AGG | 0.422149 | 5_3:-68939269 | None:intergenic |
| AGATGCAGAGAGAGGGCTGC+TGG | 0.439749 | 5_3:+68939712 | Msa0827570:CDS |
| CCTAAGAGAGGAAGGTAACA+AGG | 0.502209 | 5_3:-68939238 | None:intergenic |
| AGAAGATGATTTAGAAGTGA+AGG | 0.515399 | 5_3:+68939590 | Msa0827570:CDS |
| TGATTTAGAAGTGAAGGATG+AGG | 0.517126 | 5_3:+68939596 | Msa0827570:CDS |
| AACATTCTTATCATTGCACT+TGG | 0.519113 | 5_3:+68939285 | Msa0827570:CDS |
| TGGTGTTAGTAAAGTGGATA+AGG | 0.531732 | 5_3:+68939521 | Msa0827570:CDS |
| AAGATTAAATAAAGGTAACT+AGG | 0.532639 | 5_3:-68939261 | None:intergenic |
| GATGCAGAGAGAGGGCTGCT+GGG | 0.533020 | 5_3:+68939713 | Msa0827570:CDS |
| ATTGCACTTGGAGCTGAAGC+CGG | 0.547486 | 5_3:+68939297 | Msa0827570:CDS |
| GTATGTTGGTGTTAGTAAAG+TGG | 0.551450 | 5_3:+68939515 | Msa0827570:CDS |
| TAACTAGGCCTAAGAGAGGA+AGG | 0.551563 | 5_3:-68939246 | None:intergenic |
| ACAAAATGAAAAGAAGCCTA+AGG | 0.566232 | 5_3:+68939458 | Msa0827570:CDS |
| CAGTTGAAGATGCAGAGAGA+GGG | 0.576252 | 5_3:+68939705 | Msa0827570:CDS |
| GTAACAAGGGTGTGATCACA+AGG | 0.579955 | 5_3:-68939224 | None:intergenic |
| AGAGAACACCGACAAAAGAC+CGG | 0.585897 | 5_3:-68939316 | None:intergenic |
| GCAGTTGAAGATGCAGAGAG+AGG | 0.589013 | 5_3:+68939704 | Msa0827570:CDS |
| GAGAAGAAGATTGTGTATGT+TGG | 0.605699 | 5_3:+68939501 | Msa0827570:CDS |
| AAGGTAACTAGGCCTAAGAG+AGG | 0.633233 | 5_3:-68939250 | None:intergenic |
| CTAAGAGAGGAAGGTAACAA+GGG | 0.639907 | 5_3:-68939237 | None:intergenic |
| AAATGAAAAGAAGCCTAAGG+TGG | 0.649751 | 5_3:+68939461 | Msa0827570:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTCTATATAAAAAATACT+TGG | - | chr5_3:68939567-68939586 | None:intergenic | 15.0% |
| !! | AAGATTAAATAAAGGTAACT+AGG | - | chr5_3:68939264-68939283 | None:intergenic | 20.0% |
| !! | GAATGTTGAAGATTAAATAA+AGG | - | chr5_3:68939272-68939291 | None:intergenic | 20.0% |
| !! | TAAAAAATACTTGGCATTGA+AGG | - | chr5_3:68939558-68939577 | None:intergenic | 25.0% |
| AACATTCTTATCATTGCACT+TGG | + | chr5_3:68939285-68939304 | Msa0827570:CDS | 30.0% | |
| TGTTCTAGCATTTCATGTTT+CGG | - | chr5_3:68939384-68939403 | None:intergenic | 30.0% | |
| ACAAAATGAAAAGAAGCCTA+AGG | + | chr5_3:68939458-68939477 | Msa0827570:CDS | 30.0% | |
| AGAAGATGATTTAGAAGTGA+AGG | + | chr5_3:68939590-68939609 | Msa0827570:CDS | 30.0% | |
| GAAGTAGAAGATGAGATTTA+TGG | + | chr5_3:68939624-68939643 | Msa0827570:CDS | 30.0% | |
| ! | GATGAGATTTATGGTGTTAA+TGG | + | chr5_3:68939633-68939652 | Msa0827570:CDS | 30.0% |
| !!! | TGTTGCTTTTTGTCTTCAGA+AGG | - | chr5_3:68939345-68939364 | None:intergenic | 35.0% |
| AAATGAAAAGAAGCCTAAGG+TGG | + | chr5_3:68939461-68939480 | Msa0827570:CDS | 35.0% | |
| !! | ACAGATTTTTCAACCACCTT+AGG | - | chr5_3:68939477-68939496 | None:intergenic | 35.0% |
| GAGAAGAAGATTGTGTATGT+TGG | + | chr5_3:68939501-68939520 | Msa0827570:CDS | 35.0% | |
| !! | GTATGTTGGTGTTAGTAAAG+TGG | + | chr5_3:68939515-68939534 | Msa0827570:CDS | 35.0% |
| ! | TGGTGTTAGTAAAGTGGATA+AGG | + | chr5_3:68939521-68939540 | Msa0827570:CDS | 35.0% |
| TGATTTAGAAGTGAAGGATG+AGG | + | chr5_3:68939596-68939615 | Msa0827570:CDS | 35.0% | |
| CTAAGAGAGGAAGGTAACAA+GGG | - | chr5_3:68939240-68939259 | None:intergenic | 40.0% | |
| GCAAAAGCTGAAGCTTTCAT+TGG | + | chr5_3:68939669-68939688 | Msa0827570:CDS | 40.0% | |
| ! | GTAACAAGGGTGTGATCACA+AGG | - | chr5_3:68939227-68939246 | None:intergenic | 45.0% |
| CCTAAGAGAGGAAGGTAACA+AGG | - | chr5_3:68939241-68939260 | None:intergenic | 45.0% | |
| CCTTGTTACCTTCCTCTCTT+AGG | + | chr5_3:68939238-68939257 | Msa0827570:CDS | 45.0% | |
| TAACTAGGCCTAAGAGAGGA+AGG | - | chr5_3:68939249-68939268 | None:intergenic | 45.0% | |
| AAGGTAACTAGGCCTAAGAG+AGG | - | chr5_3:68939253-68939272 | None:intergenic | 45.0% | |
| AGAGAACACCGACAAAAGAC+CGG | - | chr5_3:68939319-68939338 | None:intergenic | 45.0% | |
| CAGTTGAAGATGCAGAGAGA+GGG | + | chr5_3:68939705-68939724 | Msa0827570:CDS | 45.0% | |
| ATTGCACTTGGAGCTGAAGC+CGG | + | chr5_3:68939297-68939316 | Msa0827570:CDS | 50.0% | |
| ! | AGCTGAAGCCGGTCTTTTGT+CGG | + | chr5_3:68939308-68939327 | Msa0827570:CDS | 50.0% |
| GCAGTTGAAGATGCAGAGAG+AGG | + | chr5_3:68939704-68939723 | Msa0827570:CDS | 50.0% | |
| AGATGCAGAGAGAGGGCTGC+TGG | + | chr5_3:68939712-68939731 | Msa0827570:CDS | 60.0% | |
| GATGCAGAGAGAGGGCTGCT+GGG | + | chr5_3:68939713-68939732 | Msa0827570:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 68939171 | 68939743 | 68939171 | ID=Msa0827570;Name=Msa0827570 |
| chr5_3 | mRNA | 68939171 | 68939743 | 68939171 | ID=Msa0827570-mRNA-1;Parent=Msa0827570;Name=Msa0827570-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|190 |
| chr5_3 | exon | 68939171 | 68939743 | 68939171 | ID=Msa0827570-mRNA-1:exon:21663;Parent=Msa0827570-mRNA-1 |
| chr5_3 | CDS | 68939171 | 68939743 | 68939171 | ID=Msa0827570-mRNA-1:cds;Parent=Msa0827570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
