AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1413130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413130 MtrunA17_Chr2g0283851 96.049 405 16 0 1 405 1 405 0.0 816
Msa1413130 MtrunA17_Chr6g0456681 49.495 396 185 10 13 400 20 408 2.41e-129 379
Msa1413130 MtrunA17_Chr6g0456691 47.816 412 177 14 14 400 21 419 4.16e-123 363
Msa1413130 MtrunA17_Chr8g0387921 42.373 354 184 9 58 401 77 420 1.97e-92 284
Msa1413130 MtrunA17_Chr8g0347321 38.889 360 191 12 56 401 96 440 3.39e-84 264
Msa1413130 MtrunA17_Chr5g0414061 40.000 355 188 11 60 402 99 440 2.93e-82 259
Msa1413130 MtrunA17_Chr6g0451321 38.649 370 203 11 45 404 106 461 8.76e-80 253
Msa1413130 MtrunA17_Chr8g0391481 37.057 367 196 14 51 400 24 372 1.31e-77 245
Msa1413130 MtrunA17_Chr7g0226991 37.398 369 198 14 50 402 53 404 4.38e-77 244
Msa1413130 MtrunA17_Chr3g0140371 36.813 364 190 13 59 404 22 363 1.19e-76 242
Msa1413130 MtrunA17_Chr5g0414091 37.640 356 197 12 60 402 90 433 1.26e-75 241
Msa1413130 MtrunA17_Chr5g0414041 39.410 373 200 14 43 402 76 435 4.95e-74 238
Msa1413130 MtrunA17_Chr7g0221281 50.000 242 112 7 168 404 3 240 4.91e-73 229
Msa1413130 MtrunA17_Chr5g0414051 34.644 407 226 18 15 402 37 422 1.95e-72 233
Msa1413130 MtrunA17_Chr1g0188191 35.165 364 204 10 56 402 235 583 1.16e-65 219
Msa1413130 MtrunA17_Chr6g0476041 34.840 376 189 16 56 402 168 516 5.98e-64 213
Msa1413130 MtrunA17_Chr7g0259711 31.608 367 207 14 56 402 92 434 2.36e-45 162
Msa1413130 MtrunA17_Chr1g0175671 30.214 374 202 13 56 403 171 511 2.00e-44 161
Msa1413130 MtrunA17_Chr4g0071391 30.663 362 211 14 56 401 110 447 5.26e-44 159
Msa1413130 MtrunA17_Chr7g0269991 32.500 360 207 12 56 404 138 472 7.55e-44 159
Msa1413130 MtrunA17_Chr7g0221271 50.327 153 66 4 35 186 62 205 3.81e-43 149
Msa1413130 MtrunA17_Chr2g0287901 30.854 363 214 12 56 402 98 439 4.19e-42 154
Msa1413130 MtrunA17_Chr7g0258851 29.947 374 203 15 56 402 249 590 8.70e-42 155
Msa1413130 MtrunA17_Chr8g0368461 31.565 377 207 16 56 404 179 532 1.75e-41 153
Msa1413130 MtrunA17_Chr3g0105301 32.308 390 187 19 32 396 35 372 6.12e-41 149
Msa1413130 MtrunA17_Chr5g0413271 29.810 369 205 12 56 404 146 480 1.99e-40 149
Msa1413130 MtrunA17_Chr4g0068111 27.701 361 230 11 53 401 113 454 6.51e-40 148
Msa1413130 MtrunA17_Chr3g0113671 30.667 375 206 15 56 404 107 453 5.48e-39 145
Msa1413130 MtrunA17_Chr2g0283581 30.789 393 225 17 20 403 6 360 7.52e-39 142
Msa1413130 MtrunA17_Chr4g0070341 28.852 357 224 12 56 400 133 471 9.63e-39 145
Msa1413130 MtrunA17_Chr3g0114101 29.584 409 241 16 9 396 44 426 1.87e-38 143
Msa1413130 MtrunA17_Chr8g0360521 28.883 367 213 16 50 396 67 405 3.24e-38 142
Msa1413130 MtrunA17_Chr4g0006991 30.053 376 207 17 51 400 82 427 3.70e-38 142
Msa1413130 MtrunA17_Chr5g0430371 30.971 381 197 19 51 401 55 399 6.45e-37 138
Msa1413130 MtrunA17_Chr7g0221291 63.736 91 32 1 56 145 80 170 8.64e-37 132
Msa1413130 MtrunA17_Chr3g0091031 30.362 359 191 15 53 396 30 344 9.36e-36 134
Msa1413130 MtrunA17_Chr8g0384151 29.178 353 215 12 56 400 91 416 1.31e-35 135
Msa1413130 MtrunA17_Chr3g0085081 30.084 359 192 13 53 396 30 344 3.92e-35 132
Msa1413130 MtrunA17_Chr1g0213281 28.649 370 217 15 56 402 176 521 7.58e-35 135
Msa1413130 MtrunA17_Chr7g0242701 29.333 375 201 16 56 400 87 427 1.85e-34 132
Msa1413130 MtrunA17_Chr7g0243301 27.404 416 249 19 9 396 15 405 2.00e-34 132
Msa1413130 MtrunA17_Chr4g0005481 28.307 378 211 16 49 400 103 446 2.06e-34 132
Msa1413130 MtrunA17_Chr5g0437601 28.455 369 217 16 50 397 79 421 5.26e-34 131
Msa1413130 MtrunA17_Chr8g0384421 27.095 358 221 11 60 400 138 472 6.75e-34 131
Msa1413130 MtrunA17_Chr5g0414081 34.314 204 115 9 214 402 2 201 1.16e-33 124
Msa1413130 MtrunA17_Chr1g0171171 29.558 362 192 15 59 400 57 375 4.09e-33 127
Msa1413130 MtrunA17_Chr4g0005491 26.781 407 264 13 14 402 20 410 1.13e-32 127
Msa1413130 MtrunA17_Chr1g0210771 30.471 361 183 16 59 396 63 378 1.15e-32 126
Msa1413130 MtrunA17_Chr3g0097891 28.351 388 222 17 21 396 3 346 2.12e-32 125
Msa1413130 MtrunA17_Chr3g0085071 27.606 355 209 11 56 400 47 363 3.52e-32 125
Msa1413130 MtrunA17_Chr2g0314801 28.652 356 207 15 56 400 34 353 4.17e-32 124
Msa1413130 MtrunA17_Chr2g0325481 28.940 349 203 13 56 396 51 362 6.10e-31 121
Msa1413130 MtrunA17_Chr5g0437291 28.571 371 217 14 52 398 162 508 3.57e-30 121
Msa1413130 MtrunA17_Chr4g0045941 29.086 361 207 16 43 393 22 343 5.16e-29 115
Msa1413130 MtrunA17_Chr6g0455901 26.630 368 210 13 60 397 76 413 6.16e-29 117
Msa1413130 MtrunA17_Chr4g0045951 27.157 394 236 15 10 393 3 355 8.47e-29 115
Msa1413130 MtrunA17_Chr4g0041011 26.823 384 244 10 20 396 6 359 1.97e-28 114
Msa1413130 MtrunA17_Chr2g0314791 28.696 345 190 16 69 402 48 347 1.74e-26 108
Msa1413130 MtrunA17_Chr4g0049421 26.611 357 239 10 56 401 49 393 2.86e-26 109
Msa1413130 MtrunA17_Chr3g0126021 26.431 367 228 13 50 402 124 462 5.99e-26 108
Msa1413130 MtrunA17_Chr5g0413851 28.093 388 225 19 26 402 17 361 9.71e-26 107
Msa1413130 MtrunA17_Chr1g0209921 38.667 150 78 4 27 164 13 160 7.07e-23 97.1
Msa1413130 MtrunA17_Chr1g0178081 26.877 253 165 7 16 255 11 256 8.98e-21 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413130 AT5G15890.1 49.608 383 162 12 32 400 157 522 3.22e-120 359
Msa1413130 AT5G15900.1 45.455 418 194 12 7 402 6 411 6.29e-117 347
Msa1413130 AT5G15900.2 45.455 418 194 12 7 402 25 430 1.01e-116 347
Msa1413130 AT3G02440.2 45.682 359 173 10 56 405 136 481 3.02e-104 317
Msa1413130 AT3G02440.1 45.682 359 173 10 56 405 128 473 3.76e-104 317
Msa1413130 AT1G01430.1 39.031 392 203 13 32 401 71 448 2.83e-89 278
Msa1413130 AT4G23790.1 38.753 369 195 12 50 401 74 428 2.14e-87 272
Msa1413130 AT4G11090.1 35.859 396 218 12 30 401 43 426 7.42e-86 268
Msa1413130 AT4G01080.1 40.395 354 181 10 56 397 92 427 1.96e-84 265
Msa1413130 AT1G70230.1 37.017 362 194 14 51 402 76 413 7.45e-78 247
Msa1413130 AT3G28150.1 36.667 360 210 12 50 403 66 413 9.08e-75 239
Msa1413130 AT4G25360.1 35.000 380 202 11 47 402 168 526 1.82e-65 218
Msa1413130 AT4G25360.2 35.000 380 202 11 47 402 168 526 1.82e-65 218
Msa1413130 AT5G51640.1 34.555 382 204 10 46 402 130 490 3.37e-65 216
Msa1413130 AT5G49340.1 31.781 365 212 11 53 401 99 442 4.23e-49 173
Msa1413130 AT5G20590.1 30.055 366 209 14 56 401 142 480 5.44e-44 159
Msa1413130 AT1G60790.1 31.452 372 208 15 56 405 191 537 3.31e-43 159
Msa1413130 AT3G12060.1 30.108 372 207 14 53 401 195 536 6.06e-43 158
Msa1413130 AT3G06080.2 32.075 371 203 16 56 403 104 448 3.32e-42 154
Msa1413130 AT3G11570.1 30.857 350 201 11 70 403 98 422 1.91e-40 149
Msa1413130 AT3G11030.1 29.781 366 214 14 52 397 103 445 7.71e-40 148
Msa1413130 AT5G19160.1 29.508 366 220 10 56 402 100 446 1.10e-39 147
Msa1413130 AT5G64020.1 29.301 372 215 12 56 400 58 408 1.92e-39 145
Msa1413130 AT3G62390.1 30.114 352 209 13 60 399 143 469 2.60e-39 147
Msa1413130 AT1G48880.1 30.163 368 212 14 47 402 107 441 7.73e-39 145
Msa1413130 AT2G40160.1 29.428 367 223 13 56 403 78 427 9.59e-39 144
Msa1413130 AT5G06700.1 28.841 371 211 14 57 403 257 598 9.84e-39 147
Msa1413130 AT2G40160.2 28.831 385 238 13 37 402 59 426 3.04e-38 143
Msa1413130 AT1G73140.1 29.067 375 214 17 50 402 63 407 6.59e-38 142
Msa1413130 AT5G06230.2 28.863 343 217 9 70 403 43 367 8.79e-38 140
Msa1413130 AT5G06230.1 28.863 343 217 9 70 403 84 408 1.76e-37 140
Msa1413130 AT5G58600.1 29.570 372 205 16 53 401 64 401 3.40e-37 139
Msa1413130 AT5G64470.3 28.184 369 233 11 46 401 46 395 4.23e-37 139
Msa1413130 AT2G14530.1 28.065 367 211 13 60 400 67 406 2.20e-36 137
Msa1413130 AT2G34070.1 30.641 359 209 14 51 403 61 385 2.34e-36 137
Msa1413130 AT2G40150.1 30.809 383 224 15 38 398 54 417 1.39e-35 135
Msa1413130 AT1G29050.1 28.371 356 210 12 56 403 62 380 1.56e-35 134
Msa1413130 AT2G30900.1 28.756 386 233 14 21 396 8 361 1.74e-35 134
Msa1413130 AT5G64470.2 27.807 374 234 11 46 401 46 401 4.24e-35 134
Msa1413130 AT2G30010.1 29.412 391 209 19 40 401 45 397 6.49e-35 133
Msa1413130 AT2G30010.2 29.412 391 209 19 40 401 88 440 1.16e-34 133
Msa1413130 AT5G20680.6 27.807 374 225 14 46 400 110 457 2.11e-34 133
Msa1413130 AT5G20680.2 27.807 374 225 14 46 400 186 533 3.04e-34 134
Msa1413130 AT5G20680.4 27.807 374 225 14 46 400 204 551 3.66e-34 134
Msa1413130 AT5G20680.1 27.807 374 225 14 46 400 204 551 3.66e-34 134
Msa1413130 AT5G20680.3 27.807 374 225 14 46 400 204 551 3.66e-34 134
Msa1413130 AT5G20680.5 27.807 374 225 14 46 400 204 551 3.66e-34 134
Msa1413130 AT3G55990.1 29.539 369 213 15 52 398 137 480 5.81e-34 132
Msa1413130 AT5G01620.2 28.010 382 219 16 44 400 99 449 1.37e-33 130
Msa1413130 AT5G01620.1 28.010 382 219 16 44 400 99 449 1.37e-33 130
Msa1413130 AT2G40320.1 27.273 385 228 17 36 397 64 419 1.64e-33 130
Msa1413130 AT5G01620.3 28.010 382 219 16 44 400 107 457 2.04e-33 130
Msa1413130 AT5G01360.2 28.302 371 215 15 51 397 32 375 2.52e-33 129
Msa1413130 AT5G01360.1 28.032 371 216 15 51 397 88 431 3.55e-33 129
Msa1413130 AT3G14850.2 29.888 358 201 14 56 400 35 355 1.15e-32 126
Msa1413130 AT2G42570.1 28.841 371 219 13 34 396 27 360 2.47e-31 123
Msa1413130 AT2G31110.2 27.945 365 208 14 47 398 37 359 1.45e-30 120
Msa1413130 AT2G38320.1 26.446 363 233 11 56 401 60 405 3.50e-30 120
Msa1413130 AT5G64470.4 28.214 280 182 7 46 315 46 316 4.42e-28 113
Msa1413130 AT5G64470.1 28.470 281 181 8 46 315 46 317 1.06e-27 112
Msa1413130 AT2G37720.1 25.654 382 226 13 45 400 133 482 1.16e-27 114
Msa1413130 AT3G54260.1 26.796 362 205 14 59 401 55 375 1.42e-27 112
Msa1413130 AT5G20590.2 31.621 253 142 10 56 293 142 378 1.89e-27 112
Msa1413130 AT5G19160.2 33.074 257 147 9 50 293 92 336 1.26e-26 109
Msa1413130 AT5G58600.2 37.297 185 93 8 53 232 64 230 1.43e-26 108
Msa1413130 AT2G38320.2 25.369 339 221 9 78 401 1 322 2.03e-26 108
Msa1413130 AT1G78710.1 27.247 356 212 13 56 401 39 357 1.46e-25 107
Msa1413130 AT3G62390.2 29.749 279 170 10 60 325 143 408 4.24e-25 106
Msa1413130 AT2G40160.3 29.658 263 170 7 37 293 59 312 6.65e-25 104
Msa1413130 AT3G06080.1 33.202 253 138 11 56 293 104 340 7.84e-25 104
Msa1413130 AT2G30900.2 35.673 171 98 6 21 186 8 171 5.13e-24 100
Msa1413130 AT5G06230.3 28.085 235 151 6 70 296 84 308 1.39e-20 92.0
Msa1413130 AT2G31110.3 34.752 141 85 2 47 186 37 171 1.09e-19 88.6
Msa1413130 AT1G78710.2 32.331 133 80 4 56 186 32 156 3.97e-14 73.2
Msa1413130 AT2G34070.2 26.761 284 169 13 125 403 2 251 5.38e-14 72.0
Msa1413130 AT3G14850.1 26.736 288 162 13 125 400 2 252 1.57e-13 70.5

Find 77 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGACATCCTAACAAATA+TGG 0.236825 tig0024012:+29797 Msa1413130:CDS
CATTCAGTAACGAACATAAA+TGG 0.238151 tig0024012:+29639 Msa1413130:CDS
GGACATGTTTGATTGTAATA+AGG 0.269234 tig0024012:-25829 None:intergenic
CCATTCACATTCTACGAAAA+TGG 0.269331 tig0024012:+29419 Msa1413130:CDS
CGACTTGTCCAAGCTGTATC+TGG 0.279200 tig0024012:-29344 None:intergenic
AAATACAATCAAACCTTAAA+AGG 0.290080 tig0024012:-30098 None:intergenic
TCGCCATTCTCGAAATGATT+TGG 0.295458 tig0024012:-29581 None:intergenic
GGGAAGAATGGTTCACGTTT+TGG 0.297933 tig0024012:+29737 Msa1413130:CDS
CTGAATTAGCAATTCTTCAT+AGG 0.322750 tig0024012:-30029 None:intergenic
GTTGTAGATGTAAGATTTAG+AGG 0.331571 tig0024012:-29279 None:intergenic
TACGAAAATGGTACAATTGT+TGG 0.334836 tig0024012:+29431 Msa1413130:CDS
AGCTGTAACAACTTGTTCTT+AGG 0.334924 tig0024012:+25781 Msa1413130:CDS
AGAATTGCTAATTCAGTATT+AGG 0.338300 tig0024012:+30036 Msa1413130:three_prime_UTR
TTCATTTGTATTATGTGATC+TGG 0.362050 tig0024012:-30059 None:intergenic
TCTCTATATGATCATGAGAA+TGG 0.372141 tig0024012:+29904 Msa1413130:CDS
ACCACCATTTGAAATATGTT+AGG 0.397436 tig0024012:-29199 None:intergenic
GGGAACAAGAAAATCCGTTT+AGG 0.398457 tig0024012:-25658 None:intergenic
AACAAATATGGGCATGTGTC+TGG 0.408166 tig0024012:+29809 Msa1413130:CDS
TGTATAATCTCTAGACCATA+TGG 0.419538 tig0024012:-29671 None:intergenic
GGTTCTACAATAGTTGTTGT+TGG 0.420927 tig0024012:-25757 None:intergenic
CTCTATATGATCATGAGAAT+GGG 0.421802 tig0024012:+29905 Msa1413130:CDS
TCAATAGAACTCAACTATTA+CGG 0.422237 tig0024012:+29473 Msa1413130:CDS
GACACATGCCCATATTTGTT+AGG 0.433571 tig0024012:-29806 None:intergenic
GCCAACCCTTTGAACAGATT+AGG 0.439293 tig0024012:-29539 None:intergenic
ATTCAGTAACGAACATAAAT+GGG 0.440120 tig0024012:+29640 Msa1413130:CDS
AGTGCTGCAAAGAACAATAC+AGG 0.440892 tig0024012:+29716 Msa1413130:CDS
CATTTATGTTCGTTACTGAA+TGG 0.441557 tig0024012:-29638 None:intergenic
TCTCCAAATCATTTCGAGAA+TGG 0.442620 tig0024012:+29578 Msa1413130:CDS
GCATTAATGATTGTGTTCAT+TGG 0.443179 tig0024012:+29843 Msa1413130:CDS
ACAGCTTGGACAAGTCGAAT+TGG 0.448449 tig0024012:+29350 Msa1413130:CDS
AAAGACAACTAATAATGTAT+TGG 0.451029 tig0024012:-25511 None:intergenic
ATCTCATTAATAGTGTCAAC+AGG 0.452614 tig0024012:-29878 None:intergenic
GATTATACATCAAATACAAT+TGG 0.453202 tig0024012:+29685 Msa1413130:CDS
AAAGGAAGTTCACAATCATC+AGG 0.459292 tig0024012:-25925 None:intergenic
TGTGTTCATTGGTGTATGCC+AGG 0.462264 tig0024012:+29854 Msa1413130:CDS
AAGACCTAACATATTTCAAA+TGG 0.463983 tig0024012:+29195 Msa1413130:CDS
ATGAATCCTAATCTGTTCAA+AGG 0.468604 tig0024012:+29533 Msa1413130:CDS
GATTGTTAAGGGAAAGTCAA+TGG 0.479240 tig0024012:+25972 Msa1413130:CDS
TCCTAATCTGTTCAAAGGGT+TGG 0.479269 tig0024012:+29538 Msa1413130:CDS
CACTTAAGAAAATCACTGTC+TGG 0.480060 tig0024012:-25892 None:intergenic
ATGGACATCCTAACAAATAT+GGG 0.489535 tig0024012:+29798 Msa1413130:CDS
TTAATAGTGTCAACAGGCCC+TGG 0.494028 tig0024012:-29872 None:intergenic
AAATGGGAGGTGCATCCATA+TGG 0.498779 tig0024012:+29656 Msa1413130:CDS
TCATCTAGATAAAGACTCTC+GGG 0.508720 tig0024012:-29320 None:intergenic
ACCTAACATATTTCAAATGG+TGG 0.511538 tig0024012:+29198 Msa1413130:CDS
TGAATCCTAATCTGTTCAAA+GGG 0.511700 tig0024012:+29534 Msa1413130:CDS
AAACAGAACTGTCTTATTCA+CGG 0.520840 tig0024012:+25865 Msa1413130:CDS
AAGAACAATACAGGGAAGAA+TGG 0.522710 tig0024012:+29725 Msa1413130:CDS
TTCATCTAGATAAAGACTCT+CGG 0.524467 tig0024012:-29321 None:intergenic
TGATTGAAGTGAATGAGAAG+AGG 0.531100 tig0024012:-25712 None:intergenic
ACAACTTGTTCTTAGGAACA+TGG 0.533263 tig0024012:+25788 Msa1413130:CDS
TATTTGTTAGGATGTCCATC+AGG 0.533554 tig0024012:-29794 None:intergenic
CTCTATTGGGAGAAATCAGA+TGG 0.539667 tig0024012:+26008 Msa1413130:CDS
GGCGAATGGAACAATGGTGG+AGG 0.551143 tig0024012:+29599 Msa1413130:CDS
CAACTTGTTCTTAGGAACAT+GGG 0.555004 tig0024012:+25789 Msa1413130:CDS
TTAAACAATGTATTGAACAA+AGG 0.560079 tig0024012:+29993 Msa1413130:three_prime_UTR
TTTCGTAGAATGTGAATGGT+CGG 0.563386 tig0024012:-29415 None:intergenic
CTTTCAGGTTGCTCGTGCTG+AGG 0.566325 tig0024012:+29142 Msa1413130:intron
TAGATGAACCAGATACAGCT+TGG 0.571908 tig0024012:+29336 Msa1413130:CDS
GTGTTCATTGGTGTATGCCA+GGG 0.577510 tig0024012:+29855 Msa1413130:CDS
ATCATTTCGAGAATGGCGAA+TGG 0.594806 tig0024012:+29585 Msa1413130:CDS
AATGTATTGAACAAAGGTGT+TGG 0.601069 tig0024012:+29999 Msa1413130:three_prime_UTR
TTATTATTATCATAGAGTCA+TGG 0.608810 tig0024012:+25606 Msa1413130:exon
AAAGGAGAATTTCTAGCACT+AGG 0.608854 tig0024012:-30145 None:intergenic
AAGAACAAGTTGTTACAGCT+AGG 0.609399 tig0024012:-25778 None:intergenic
AACTGAGTTGCATCAAACAA+AGG 0.611259 tig0024012:-25943 None:intergenic
GCAATGTTGATGAGACCTGA+TGG 0.619929 tig0024012:+29779 Msa1413130:CDS
AGGGGACCATAGCATTGCAA+TGG 0.626452 tig0024012:-29243 None:intergenic
CAAGAATGAGTACTGTGAGT+GGG 0.638314 tig0024012:-25678 None:intergenic
GAGAATGGCGAATGGAACAA+TGG 0.644400 tig0024012:+29593 Msa1413130:CDS
ACAAGAATGAGTACTGTGAG+TGG 0.651564 tig0024012:-25679 None:intergenic
GCGAATGGAACAATGGTGGA+GGG 0.654886 tig0024012:+29600 Msa1413130:CDS
TAGAGTCATGGAGTCATGTG+AGG 0.667840 tig0024012:+25618 Msa1413130:exon
AATGGCGAATGGAACAATGG+TGG 0.671698 tig0024012:+29596 Msa1413130:CDS
CAGTAACGAACATAAATGGG+AGG 0.676804 tig0024012:+29643 Msa1413130:CDS
GTGCTGCAAAGAACAATACA+GGG 0.679216 tig0024012:+29717 Msa1413130:CDS
GTATTGGAAACTGCTCAACG+TGG 0.779757 tig0024012:-25495 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAAGCAAAAAATAT+AGG - tig0024012:26091-26110 None:intergenic 10.0%
!!! TTTTTGTTCATATAAATTTT+TGG + tig0024012:26174-26193 Msa1413130:intron 10.0%
!!! AAAATTTGCTTATTTATTTT+TGG - tig0024012:26396-26415 None:intergenic 10.0%
!! GTTTATTATGTTTAATTAAA+AGG + tig0024012:26510-26529 Msa1413130:intron 10.0%
!!! TTTTTTTTTTTTTTTGTAGA+TGG + tig0024012:26951-26970 Msa1413130:intron 10.0%
!! TAAATAATTAAGGATATTAT+AGG + tig0024012:28004-28023 Msa1413130:intron 10.0%
!! TTAGTCAAAAACAAATAAAA+GGG - tig0024012:25539-25558 None:intergenic 15.0%
!! ATTAGTCAAAAACAAATAAA+AGG - tig0024012:25540-25559 None:intergenic 15.0%
!!! TTGCTTTAATTTTTGTTTAA+AGG + tig0024012:26098-26117 Msa1413130:intron 15.0%
!!! AAAAAAAAATTCTGAAATAG+AGG - tig0024012:26248-26267 None:intergenic 15.0%
!! AAATTATCAAAATTATGAAC+TGG + tig0024012:26269-26288 Msa1413130:intron 15.0%
!!! ATTTAAGTCAAACTTTTTTT+GGG + tig0024012:26338-26357 Msa1413130:intron 15.0%
!! AAAAAAACATTATCTCAATA+AGG - tig0024012:26928-26947 None:intergenic 15.0%
!!! AAAATTTGTAAGTTTTTGTT+TGG - tig0024012:27120-27139 None:intergenic 15.0%
!!! AATAAATAAGGGTATAATAA+TGG + tig0024012:27474-27493 Msa1413130:intron 15.0%
!! AAAAATAATAAATGTTGCAT+TGG + tig0024012:27499-27518 Msa1413130:intron 15.0%
!!! TATTTTGAGAAAAAGAAAAA+TGG + tig0024012:27539-27558 Msa1413130:intron 15.0%
!!! ATTTTGAGAAAAAGAAAAAT+GGG + tig0024012:27540-27559 Msa1413130:intron 15.0%
!!! AAGTATATATACAACATTTT+GGG - tig0024012:28261-28280 None:intergenic 15.0%
!!! AAAGTATATATACAACATTT+TGG - tig0024012:28262-28281 None:intergenic 15.0%
!!! ATATATACTTTTCTAATTAG+AGG + tig0024012:28271-28290 Msa1413130:intron 15.0%
!!! TTTTGTACTCAATATATTTA+AGG + tig0024012:28305-28324 Msa1413130:intron 15.0%
!! GTATAAGTAAAAATAATTAG+AGG + tig0024012:28357-28376 Msa1413130:intron 15.0%
!!! TTTTGTACTCAATATATTTA+AGG + tig0024012:28305-28324 Msa1413130:intron 15.0%
!! TTACTTATATAAAACATTAG+AGG - tig0024012:28433-28452 None:intergenic 15.0%
!!! GCTTAAATTAAGTTTTATTT+AGG - tig0024012:29117-29136 None:intergenic 15.0%
!!! AAATTTGTATACTTTTGTTT+TGG + tig0024012:29957-29976 Msa1413130:three_prime_UTR 15.0%
!! AAAGACAACTAATAATGTAT+TGG - tig0024012:25514-25533 None:intergenic 20.0%
!! TTATTATTATCATAGAGTCA+TGG + tig0024012:25606-25625 Msa1413130:exon 20.0%
!!! TAATAAGGTTGTTTTAGATA+AGG - tig0024012:25817-25836 None:intergenic 20.0%
!!! TTCTGTTTTTCAGTTATAAA+AGG - tig0024012:25853-25872 None:intergenic 20.0%
!!! GTTTTCCATTTCAAAAAAAA+TGG - tig0024012:26295-26314 None:intergenic 20.0%
!!! CATTTAAGTCAAACTTTTTT+TGG + tig0024012:26337-26356 Msa1413130:intron 20.0%
!!! AAGAAAAAACACTTTTCAAA+TGG + tig0024012:26461-26480 Msa1413130:intron 20.0%
!!! AATTTTTCATCTAACACATT+TGG - tig0024012:26557-26576 None:intergenic 20.0%
!! ACACATTGACAAAAAAATAT+TGG + tig0024012:26583-26602 Msa1413130:intron 20.0%
!!! ACAAAAAAATATTGGCTTTT+AGG + tig0024012:26591-26610 Msa1413130:intron 20.0%
!!! TTTTTGTTTGGAAATTTGAA+AGG + tig0024012:26697-26716 Msa1413130:intron 20.0%
!!! AACGGTTGATATTATTTAAT+TGG + tig0024012:26806-26825 Msa1413130:intron 20.0%
!!! TTTTAACTTTGTTGTAATGA+AGG + tig0024012:27136-27155 Msa1413130:intron 20.0%
!!! TTTAACTTTGTTGTAATGAA+GGG + tig0024012:27137-27156 Msa1413130:intron 20.0%
!!! ATTAGTTTTGTTGATTTTGA+TGG + tig0024012:27376-27395 Msa1413130:intron 20.0%
!! TGGATGAAATTCAATAAATA+AGG + tig0024012:27462-27481 Msa1413130:intron 20.0%
!! GGATGAAATTCAATAAATAA+GGG + tig0024012:27463-27482 Msa1413130:intron 20.0%
!! TCAAAATGTGACAATTATAA+AGG + tig0024012:28091-28110 Msa1413130:intron 20.0%
!! CAACAAAGAAAATCTATATT+GGG - tig0024012:28154-28173 None:intergenic 20.0%
!! ACAACAAAGAAAATCTATAT+TGG - tig0024012:28155-28174 None:intergenic 20.0%
!! TTACTTATACAAGAAATTAG+AGG - tig0024012:28347-28366 None:intergenic 20.0%
!! TTCAAATTAAGATGTGTTTA+AGG + tig0024012:28619-28638 Msa1413130:intron 20.0%
!! TTTCTTTCACTATCATAATA+TGG + tig0024012:29053-29072 Msa1413130:intron 20.0%
!! GATTATACATCAAATACAAT+TGG + tig0024012:29685-29704 Msa1413130:CDS 20.0%
!! TTAAACAATGTATTGAACAA+AGG + tig0024012:29993-30012 Msa1413130:three_prime_UTR 20.0%
!!! AATGATTAATTCTCCTTTTA+AGG + tig0024012:30085-30104 Msa1413130:three_prime_UTR 20.0%
!! AAATACAATCAAACCTTAAA+AGG - tig0024012:30101-30120 None:intergenic 20.0%
!! TACTTGACAAAAAAAAAACT+CGG - tig0024012:30124-30143 None:intergenic 20.0%
!!! TCAGTTTTTCAAGATTGTTA+AGG + tig0024012:25960-25979 Msa1413130:CDS 25.0%
!!! CAGTTTTTCAAGATTGTTAA+GGG + tig0024012:25961-25980 Msa1413130:CDS 25.0%
! AAACAACCAAACTAATATTG+GGG - tig0024012:26150-26169 None:intergenic 25.0%
! CAAACAACCAAACTAATATT+GGG - tig0024012:26151-26170 None:intergenic 25.0%
! TCAAACAACCAAACTAATAT+TGG - tig0024012:26152-26171 None:intergenic 25.0%
! GAAGAAAACAAATATAAGGT+GGG - tig0024012:26667-26686 None:intergenic 25.0%
! ATGCGAAGAAAACAAATATA+AGG - tig0024012:26671-26690 None:intergenic 25.0%
! AACCGTTAATTTAAACTAGA+TGG - tig0024012:26793-26812 None:intergenic 25.0%
! GACTAATTGTGTAATATTCA+TGG + tig0024012:26832-26851 Msa1413130:intron 25.0%
! TTGAAAAAAGTAAGGAGAAA+AGG - tig0024012:27077-27096 None:intergenic 25.0%
!!! TTTTGTTTGGAAGTTTAGTA+GGG - tig0024012:27107-27126 None:intergenic 25.0%
!!! TTTTTGTTTGGAAGTTTAGT+AGG - tig0024012:27108-27127 None:intergenic 25.0%
!! TTAACTTTGTTGTAATGAAG+GGG + tig0024012:27138-27157 Msa1413130:intron 25.0%
! CCTATTATAGGAAGATAATT+TGG - tig0024012:27225-27244 None:intergenic 25.0%
! CCAAATTATCTTCCTATAAT+AGG + tig0024012:27222-27241 Msa1413130:intron 25.0%
! AAACGACTATTATTTAGAGA+CGG - tig0024012:27327-27346 None:intergenic 25.0%
! CTCTAAATAATAGTCGTTTA+AGG + tig0024012:27328-27347 Msa1413130:intron 25.0%
! CCACTAACTACAATTAATAA+GGG - tig0024012:27428-27447 None:intergenic 25.0%
! ACCACTAACTACAATTAATA+AGG - tig0024012:27429-27448 None:intergenic 25.0%
! TTATTAATTGTAGTTAGTGG+TGG + tig0024012:27428-27447 Msa1413130:intron 25.0%
! AAATAGGAACCATTAAATCA+AGG + tig0024012:27598-27617 Msa1413130:intron 25.0%
! TGTTATTGGACTAAACAATT+GGG - tig0024012:27655-27674 None:intergenic 25.0%
! GCGTTTATTTAAGAAAATGT+TGG - tig0024012:27717-27736 None:intergenic 25.0%
! TGAAAAAGTGACAGTTATAA+AGG - tig0024012:27866-27885 None:intergenic 25.0%
! CTCTTGCAATAAATTACTAA+GGG - tig0024012:27962-27981 None:intergenic 25.0%
! TCTCTTGCAATAAATTACTA+AGG - tig0024012:27963-27982 None:intergenic 25.0%
!! AACCTCATTTGTTAAATGTT+CGG + tig0024012:28477-28496 Msa1413130:intron 25.0%
! AAATTAAGATGTGTTTAAGG+TGG + tig0024012:28622-28641 Msa1413130:intron 25.0%
!! TTAGGTTTAAAATGAAGCAT+TGG + tig0024012:28648-28667 Msa1413130:intron 25.0%
!!! CATATATTACTGTTTTGAAC+AGG - tig0024012:28949-28968 None:intergenic 25.0%
! AAAGAGATACTTGAAAGAAT+TGG - tig0024012:29008-29027 None:intergenic 25.0%
! AAGACCTAACATATTTCAAA+TGG + tig0024012:29195-29214 Msa1413130:CDS 25.0%
!! TAGAGGATTTTACTAGAAAA+GGG - tig0024012:29265-29284 None:intergenic 25.0%
!! TTAGAGGATTTTACTAGAAA+AGG - tig0024012:29266-29285 None:intergenic 25.0%
! TCAATAGAACTCAACTATTA+CGG + tig0024012:29473-29492 Msa1413130:CDS 25.0%
! ATTCAGTAACGAACATAAAT+GGG + tig0024012:29640-29659 Msa1413130:CDS 25.0%
! AGAATTGCTAATTCAGTATT+AGG + tig0024012:30036-30055 Msa1413130:three_prime_UTR 25.0%
! TTCATTTGTATTATGTGATC+TGG - tig0024012:30062-30081 None:intergenic 25.0%
! CAGAGATTTTCAAACCTAAA+CGG + tig0024012:25644-25663 Msa1413130:CDS 30.0%
GGACATGTTTGATTGTAATA+AGG - tig0024012:25832-25851 None:intergenic 30.0%
! AAACAGAACTGTCTTATTCA+CGG + tig0024012:25865-25884 Msa1413130:CDS 30.0%
!!! TTTTTGGCTAAATGCAGTAT+TGG + tig0024012:26190-26209 Msa1413130:intron 30.0%
!!! ACTGGCCATTTTTTTTGAAA+TGG + tig0024012:26287-26306 Msa1413130:intron 30.0%
TTTGGGCAAATTTGTCATAT+AGG + tig0024012:26355-26374 Msa1413130:intron 30.0%
AATAGTACTAGAAGAAATCG+AGG + tig0024012:26640-26659 Msa1413130:intron 30.0%
CGAAGAAAACAAATATAAGG+TGG - tig0024012:26668-26687 None:intergenic 30.0%
!! TCGCATCTGAAATTTTTGTT+TGG + tig0024012:26685-26704 Msa1413130:intron 30.0%
TGTTTGGAAATTTGAAAGGA+AGG + tig0024012:26701-26720 Msa1413130:intron 30.0%
GTTTGGAAATTTGAAAGGAA+GGG + tig0024012:26702-26721 Msa1413130:intron 30.0%
TTTGGAAATTTGAAAGGAAG+GGG + tig0024012:26703-26722 Msa1413130:intron 30.0%
! GGCCATCTAGTTTAAATTAA+CGG + tig0024012:26788-26807 Msa1413130:intron 30.0%
!! CTAGATTTGTTGTTGATTTG+AGG - tig0024012:26863-26882 None:intergenic 30.0%
CTCAAATCAACAACAAATCT+AGG + tig0024012:26861-26880 Msa1413130:intron 30.0%
!! GAGAGCTTTTGAAAAAAGTA+AGG - tig0024012:27085-27104 None:intergenic 30.0%
TGTCAACTTGAAAACTTGAA+AGG + tig0024012:27181-27200 Msa1413130:intron 30.0%
GTCAACTTGAAAACTTGAAA+GGG + tig0024012:27182-27201 Msa1413130:intron 30.0%
ATGCATACTACTCCTATTAT+AGG - tig0024012:27237-27256 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG + tig0024012:27425-27444 Msa1413130:intron 30.0%
AAGAGACAATTATTACGAGA+CGG + tig0024012:27565-27584 Msa1413130:intron 30.0%
TAAACAATTGGGATAGTATG+GGG - tig0024012:27644-27663 None:intergenic 30.0%
CTAAACAATTGGGATAGTAT+GGG - tig0024012:27645-27664 None:intergenic 30.0%
ACTAAACAATTGGGATAGTA+TGG - tig0024012:27646-27665 None:intergenic 30.0%
! GTGTTATTGGACTAAACAAT+TGG - tig0024012:27656-27675 None:intergenic 30.0%
TTTCAGCTTAGCAAAGAAAA+CGG - tig0024012:27678-27697 None:intergenic 30.0%
AAGTGACAGTTATAAAGGAA+CGG - tig0024012:27861-27880 None:intergenic 30.0%
!! TAAATTACTAAGGGCGTTAT+AGG - tig0024012:27953-27972 None:intergenic 30.0%
GTGAAATACATTTAGTGTCT+TGG + tig0024012:28055-28074 Msa1413130:intron 30.0%
TGACAATTATAAAGGAACAG+AGG + tig0024012:28099-28118 Msa1413130:intron 30.0%
ATCCGAACATTTAACAAATG+AGG - tig0024012:28482-28501 None:intergenic 30.0%
!! TAGCTTTGAAGATGTGTTAT+TGG + tig0024012:28687-28706 Msa1413130:intron 30.0%
!! AGCTTTGAAGATGTGTTATT+GGG + tig0024012:28688-28707 Msa1413130:intron 30.0%
CTGAACAATTAGGAGAATTA+GGG + tig0024012:28718-28737 Msa1413130:intron 30.0%
AATTAGGAGAATTAGGGTAT+AGG + tig0024012:28724-28743 Msa1413130:intron 30.0%
ATTAGGAGAATTAGGGTATA+GGG + tig0024012:28725-28744 Msa1413130:intron 30.0%
ACCTAACATATTTCAAATGG+TGG + tig0024012:29198-29217 Msa1413130:CDS 30.0%
! ACCACCATTTGAAATATGTT+AGG - tig0024012:29202-29221 None:intergenic 30.0%
! ATTTTACCATTGCAATGCTA+TGG + tig0024012:29237-29256 Msa1413130:CDS 30.0%
! AGAGGATTTTACTAGAAAAG+GGG - tig0024012:29264-29283 None:intergenic 30.0%
GTTGTAGATGTAAGATTTAG+AGG - tig0024012:29282-29301 None:intergenic 30.0%
TTCATCTAGATAAAGACTCT+CGG - tig0024012:29324-29343 None:intergenic 30.0%
TACGAAAATGGTACAATTGT+TGG + tig0024012:29431-29450 Msa1413130:CDS 30.0%
ATGAATCCTAATCTGTTCAA+AGG + tig0024012:29533-29552 Msa1413130:CDS 30.0%
TGAATCCTAATCTGTTCAAA+GGG + tig0024012:29534-29553 Msa1413130:CDS 30.0%
CATTTATGTTCGTTACTGAA+TGG - tig0024012:29641-29660 None:intergenic 30.0%
CATTCAGTAACGAACATAAA+TGG + tig0024012:29639-29658 Msa1413130:CDS 30.0%
TGTATAATCTCTAGACCATA+TGG - tig0024012:29674-29693 None:intergenic 30.0%
ATGGACATCCTAACAAATAT+GGG + tig0024012:29798-29817 Msa1413130:CDS 30.0%
GCATTAATGATTGTGTTCAT+TGG + tig0024012:29843-29862 Msa1413130:CDS 30.0%
ATCTCATTAATAGTGTCAAC+AGG - tig0024012:29881-29900 None:intergenic 30.0%
TCTCTATATGATCATGAGAA+TGG + tig0024012:29904-29923 Msa1413130:CDS 30.0%
CTCTATATGATCATGAGAAT+GGG + tig0024012:29905-29924 Msa1413130:CDS 30.0%
! AATGTATTGAACAAAGGTGT+TGG + tig0024012:29999-30018 Msa1413130:three_prime_UTR 30.0%
CTGAATTAGCAATTCTTCAT+AGG - tig0024012:30032-30051 None:intergenic 30.0%
!!! CTGCAATCGAAGTTTTAAAC+AGG - tig0024012:25456-25475 None:intergenic 35.0%
TGATTGAAGTGAATGAGAAG+AGG - tig0024012:25715-25734 None:intergenic 35.0%
GGTTCTACAATAGTTGTTGT+TGG - tig0024012:25760-25779 None:intergenic 35.0%
AAGAACAAGTTGTTACAGCT+AGG - tig0024012:25781-25800 None:intergenic 35.0%
! AGCTGTAACAACTTGTTCTT+AGG + tig0024012:25781-25800 Msa1413130:CDS 35.0%
! ACAACTTGTTCTTAGGAACA+TGG + tig0024012:25788-25807 Msa1413130:CDS 35.0%
! CAACTTGTTCTTAGGAACAT+GGG + tig0024012:25789-25808 Msa1413130:CDS 35.0%
CACTTAAGAAAATCACTGTC+TGG - tig0024012:25895-25914 None:intergenic 35.0%
! CAGACAGTGATTTTCTTAAG+TGG + tig0024012:25893-25912 Msa1413130:CDS 35.0%
! GTGATTTTCTTAAGTGGAGA+TGG + tig0024012:25899-25918 Msa1413130:CDS 35.0%
AAAGGAAGTTCACAATCATC+AGG - tig0024012:25928-25947 None:intergenic 35.0%
AACTGAGTTGCATCAAACAA+AGG - tig0024012:25946-25965 None:intergenic 35.0%
GATTGTTAAGGGAAAGTCAA+TGG + tig0024012:25972-25991 Msa1413130:CDS 35.0%
GTCTATCCCCAATATTAGTT+TGG + tig0024012:26141-26160 Msa1413130:intron 35.0%
TCAGATTTAGTGCTATAGCT+TGG + tig0024012:26746-26765 Msa1413130:intron 35.0%
ACAAATCTAGGTCTAGATTG+TGG + tig0024012:26873-26892 Msa1413130:intron 35.0%
CAAATCTAGGTCTAGATTGT+GGG + tig0024012:26874-26893 Msa1413130:intron 35.0%
AAATCTAGGTCTAGATTGTG+GGG + tig0024012:26875-26894 Msa1413130:intron 35.0%
AATTCCTCCATTGAAGTTGA+AGG + tig0024012:27032-27051 Msa1413130:intron 35.0%
AGATAGACCTTCAACTTCAA+TGG - tig0024012:27042-27061 None:intergenic 35.0%
!!! GTTTTCAAGTTGACAAGTAC+TGG - tig0024012:27176-27195 None:intergenic 35.0%
TTATCTCCATGTGCATTGTA+CGG - tig0024012:27273-27292 None:intergenic 35.0%
GACTATTATTTAGAGACGGA+GGG - tig0024012:27323-27342 None:intergenic 35.0%
AGGTTTATGCACGCTTATTA+AGG + tig0024012:27348-27367 Msa1413130:intron 35.0%
AGGAACCATTAAATCAAGGT+CGG + tig0024012:27602-27621 Msa1413130:intron 35.0%
!! AGCAAAGAAAACGGTGTTAT+TGG - tig0024012:27669-27688 None:intergenic 35.0%
! TACGATGGTACTCTCTTTTT+AGG - tig0024012:28209-28228 None:intergenic 35.0%
! AATGTTCGGATCTAGAACTT+CGG + tig0024012:28491-28510 Msa1413130:intron 35.0%
! ATGTGTTTAAGGTGGTGATT+AGG + tig0024012:28630-28649 Msa1413130:intron 35.0%
! TGAAGATGTGTTATTGGGTT+AGG + tig0024012:28693-28712 Msa1413130:intron 35.0%
! GAAGATGTGTTATTGGGTTA+GGG + tig0024012:28694-28713 Msa1413130:intron 35.0%
CCTAATTCTCCTAATTGTTC+AGG - tig0024012:28720-28739 None:intergenic 35.0%
CCTGAACAATTAGGAGAATT+AGG + tig0024012:28717-28736 Msa1413130:intron 35.0%
AGTTGTCCAAGAGACAATTT+GGG - tig0024012:28753-28772 None:intergenic 35.0%
CTGAATCACTAACTCAATGA+AGG - tig0024012:28894-28913 None:intergenic 35.0%
CTTCATTGAGTTAGTGATTC+AGG + tig0024012:28892-28911 Msa1413130:intron 35.0%
TAATTTAAGCACACCCTTTC+AGG + tig0024012:29127-29146 Msa1413130:intron 35.0%
TCATCTAGATAAAGACTCTC+GGG - tig0024012:29323-29342 None:intergenic 35.0%
! TTCGACTATGTGATTTTCTC+CGG + tig0024012:29377-29396 Msa1413130:CDS 35.0%
TTTCGTAGAATGTGAATGGT+CGG - tig0024012:29418-29437 None:intergenic 35.0%
! CCATTTTCGTAGAATGTGAA+TGG - tig0024012:29422-29441 None:intergenic 35.0%
CCATTCACATTCTACGAAAA+TGG + tig0024012:29419-29438 Msa1413130:CDS 35.0%
TCTCCAAATCATTTCGAGAA+TGG + tig0024012:29578-29597 Msa1413130:CDS 35.0%
AAGAACAATACAGGGAAGAA+TGG + tig0024012:29725-29744 Msa1413130:CDS 35.0%
! TATTTGTTAGGATGTCCATC+AGG - tig0024012:29797-29816 None:intergenic 35.0%
GATGGACATCCTAACAAATA+TGG + tig0024012:29797-29816 Msa1413130:CDS 35.0%
GGGAACAAGAAAATCCGTTT+AGG - tig0024012:25661-25680 None:intergenic 40.0%
! CAAGAATGAGTACTGTGAGT+GGG - tig0024012:25681-25700 None:intergenic 40.0%
! ACAAGAATGAGTACTGTGAG+TGG - tig0024012:25682-25701 None:intergenic 40.0%
! GGAAAGTCAATGGCTTTTGT+CGG + tig0024012:25982-26001 Msa1413130:CDS 40.0%
! CTTTTGTCGGTGACTCTATT+GGG + tig0024012:25995-26014 Msa1413130:CDS 40.0%
CTCTATTGGGAGAAATCAGA+TGG + tig0024012:26008-26027 Msa1413130:CDS 40.0%
GGGCAAATTTGTCATATAGG+AGG + tig0024012:26358-26377 Msa1413130:intron 40.0%
TAGACCTTCAACTTCAATGG+AGG - tig0024012:27039-27058 None:intergenic 40.0%
! CTTTGTTGTAATGAAGGGGT+TGG + tig0024012:27142-27161 Msa1413130:intron 40.0%
TGCACATGGAGATAACAAAG+TGG + tig0024012:27278-27297 Msa1413130:intron 40.0%
CGACTATTATTTAGAGACGG+AGG - tig0024012:27324-27343 None:intergenic 40.0%
! TAGTGGTGGAGTAGTTAAGT+TGG + tig0024012:27442-27461 Msa1413130:intron 40.0%
AGACAATTATTACGAGACGG+AGG + tig0024012:27568-27587 Msa1413130:intron 40.0%
GACAATTATTACGAGACGGA+GGG + tig0024012:27569-27588 Msa1413130:intron 40.0%
AGACGGAGGGAGTAATAAAT+AGG + tig0024012:27582-27601 Msa1413130:intron 40.0%
! TTGCACCGACCTTGATTTAA+TGG - tig0024012:27610-27629 None:intergenic 40.0%
GACAGTTATAAAGGAACGGA+GGG - tig0024012:27857-27876 None:intergenic 40.0%
TGACAGTTATAAAGGAACGG+AGG - tig0024012:27858-27877 None:intergenic 40.0%
TAGTCATGTTAGCTGTGTCA+TGG + tig0024012:28227-28246 Msa1413130:intron 40.0%
GTTGTCCAAGAGACAATTTG+GGG - tig0024012:28752-28771 None:intergenic 40.0%
CAGTTGTCCAAGAGACAATT+TGG - tig0024012:28754-28773 None:intergenic 40.0%
CTGTCTTCAAGCATAACTGT+AGG + tig0024012:28771-28790 Msa1413130:intron 40.0%
! TTGATCAGATGGTCTTGACT+CGG + tig0024012:28830-28849 Msa1413130:intron 40.0%
ATCACATAGACTTGTCCTAG+TGG - tig0024012:28868-28887 None:intergenic 40.0%
ACTAGGACAAGTCTATGTGA+TGG + tig0024012:28867-28886 Msa1413130:intron 40.0%
AAGCCCATTTCCTTAACGAA+CGG - tig0024012:28923-28942 None:intergenic 40.0%
! TTCGTTAAGGAAATGGGCTT+CGG + tig0024012:28923-28942 Msa1413130:intron 40.0%
TAGATGAACCAGATACAGCT+TGG + tig0024012:29336-29355 Msa1413130:CDS 40.0%
TCCTAATCTGTTCAAAGGGT+TGG + tig0024012:29538-29557 Msa1413130:CDS 40.0%
!! GTTCAAAGGGTTGGCATTTT+TGG + tig0024012:29547-29566 Msa1413130:CDS 40.0%
TCGCCATTCTCGAAATGATT+TGG - tig0024012:29584-29603 None:intergenic 40.0%
ATCATTTCGAGAATGGCGAA+TGG + tig0024012:29585-29604 Msa1413130:CDS 40.0%
CAGTAACGAACATAAATGGG+AGG + tig0024012:29643-29662 Msa1413130:CDS 40.0%
AGTGCTGCAAAGAACAATAC+AGG + tig0024012:29716-29735 Msa1413130:CDS 40.0%
GTGCTGCAAAGAACAATACA+GGG + tig0024012:29717-29736 Msa1413130:CDS 40.0%
GACACATGCCCATATTTGTT+AGG - tig0024012:29809-29828 None:intergenic 40.0%
AACAAATATGGGCATGTGTC+TGG + tig0024012:29809-29828 Msa1413130:CDS 40.0%
GTATTGGAAACTGCTCAACG+TGG - tig0024012:25498-25517 None:intergenic 45.0%
TAGAGTCATGGAGTCATGTG+AGG + tig0024012:25618-25637 Msa1413130:exon 45.0%
! GCTTTTGTCGGTGACTCTAT+TGG + tig0024012:25994-26013 Msa1413130:CDS 45.0%
TTTGAAAGGAAGGGGAAGAG+AGG + tig0024012:26711-26730 Msa1413130:intron 45.0%
TTGAAAGGAAGGGGAAGAGA+GGG + tig0024012:26712-26731 Msa1413130:intron 45.0%
! TAGTGCTATAGCTTGGTGAC+AGG + tig0024012:26753-26772 Msa1413130:intron 45.0%
! AGTGCTATAGCTTGGTGACA+GGG + tig0024012:26754-26773 Msa1413130:intron 45.0%
!! GGAACAGAGGAAGTATTGTC+TGG + tig0024012:28112-28131 Msa1413130:intron 45.0%
CACAGCTAACATGACTACGA+TGG - tig0024012:28224-28243 None:intergenic 45.0%
AAGCAAGCTCCTCAGTCTTT+CGG + tig0024012:28529-28548 Msa1413130:intron 45.0%
ACCATCTGATCAAGTCGAGA+CGG - tig0024012:28823-28842 None:intergenic 45.0%
!!! GGTCTTGACTCGGTCTTTTA+TGG + tig0024012:28840-28859 Msa1413130:intron 45.0%
! CGGTCTTTTATGGTTCCACT+AGG + tig0024012:28850-28869 Msa1413130:intron 45.0%
TGACCCGTTCGTTAAGGAAA+TGG + tig0024012:28916-28935 Msa1413130:intron 45.0%
GACCCGTTCGTTAAGGAAAT+GGG + tig0024012:28917-28936 Msa1413130:intron 45.0%
AGCCCATTTCCTTAACGAAC+GGG - tig0024012:28922-28941 None:intergenic 45.0%
ACAGCTTGGACAAGTCGAAT+TGG + tig0024012:29350-29369 Msa1413130:CDS 45.0%
! GACTATGTGATTTTCTCCGG+TGG + tig0024012:29380-29399 Msa1413130:CDS 45.0%
! TGATTTTCTCCGGTGGACAA+TGG + tig0024012:29387-29406 Msa1413130:CDS 45.0%
TCGGAAAAACCATTGTCCAC+CGG - tig0024012:29399-29418 None:intergenic 45.0%
GCCAACCCTTTGAACAGATT+AGG - tig0024012:29542-29561 None:intergenic 45.0%
GAGAATGGCGAATGGAACAA+TGG + tig0024012:29593-29612 Msa1413130:CDS 45.0%
AATGGCGAATGGAACAATGG+TGG + tig0024012:29596-29615 Msa1413130:CDS 45.0%
! AAATGGGAGGTGCATCCATA+TGG + tig0024012:29656-29675 Msa1413130:CDS 45.0%
! GGGAAGAATGGTTCACGTTT+TGG + tig0024012:29737-29756 Msa1413130:CDS 45.0%
! GCAATGTTGATGAGACCTGA+TGG + tig0024012:29779-29798 Msa1413130:CDS 45.0%
TGTGTTCATTGGTGTATGCC+AGG + tig0024012:29854-29873 Msa1413130:CDS 45.0%
GTGTTCATTGGTGTATGCCA+GGG + tig0024012:29855-29874 Msa1413130:CDS 45.0%
TTAATAGTGTCAACAGGCCC+TGG - tig0024012:29875-29894 None:intergenic 45.0%
!! AATATGATATTAAATAATTA+AGG + tig0024012:27994-28013 Msa1413130:intron 5.0%
GGTGACAGGGTTATAGCTGA+CGG + tig0024012:26767-26786 Msa1413130:intron 50.0%
TCTAGGTCTAGATTGTGGGG+AGG + tig0024012:26878-26897 Msa1413130:intron 50.0%
CAACCACCGTACAATGCACA+TGG + tig0024012:27264-27283 Msa1413130:intron 50.0%
TCTCCATGTGCATTGTACGG+TGG - tig0024012:27270-27289 None:intergenic 50.0%
! GGATCTAGAACTTCGGACAG+CGG + tig0024012:28498-28517 Msa1413130:intron 50.0%
CTCCTCAGTCTTTCGGACAT+TGG + tig0024012:28536-28555 Msa1413130:intron 50.0%
CACCAATGTCCGAAAGACTG+AGG - tig0024012:28541-28560 None:intergenic 50.0%
! GTCTTTCGGACATTGGTGTC+TGG + tig0024012:28543-28562 Msa1413130:intron 50.0%
! TTCGGACATTGGTGTCTGGT+CGG + tig0024012:28547-28566 Msa1413130:intron 50.0%
TACATATACACCCCCTCCTC+CGG - tig0024012:28595-28614 None:intergenic 50.0%
GGGTTAGGGCCTGAACAATT+AGG + tig0024012:28708-28727 Msa1413130:intron 50.0%
AGGGACCCCAAATTGTCTCT+TGG + tig0024012:28744-28763 Msa1413130:intron 50.0%
ATCAAGTCGAGACGGTCTCA+TGG - tig0024012:28815-28834 None:intergenic 50.0%
! ACCGTCTCGACTTGATCAGA+TGG + tig0024012:28819-28838 Msa1413130:intron 50.0%
TCAGGATGACCCGTTCGTTA+AGG + tig0024012:28910-28929 Msa1413130:intron 50.0%
TCAGCACGAGCAACCTGAAA+GGG - tig0024012:29143-29162 None:intergenic 50.0%
AGGGGACCATAGCATTGCAA+TGG - tig0024012:29246-29265 None:intergenic 50.0%
CGACTTGTCCAAGCTGTATC+TGG - tig0024012:29347-29366 None:intergenic 50.0%
! GCGAATGGAACAATGGTGGA+GGG + tig0024012:29600-29619 Msa1413130:CDS 50.0%
! ACTGAGGAGCTTGCTTCCAC+TGG - tig0024012:28525-28544 None:intergenic 55.0%
! TCGGACATTGGTGTCTGGTC+GGG + tig0024012:28548-28567 Msa1413130:intron 55.0%
TCTGGTCGGGTATGCGTCTT+TGG + tig0024012:28561-28580 Msa1413130:intron 55.0%
TGCGTCTTTGGTCCGAAGAC+CGG + tig0024012:28573-28592 Msa1413130:intron 55.0%
CTCAGCACGAGCAACCTGAA+AGG - tig0024012:29144-29163 None:intergenic 55.0%
!! CTTTCAGGTTGCTCGTGCTG+AGG + tig0024012:29142-29161 Msa1413130:intron 55.0%
GGCGAATGGAACAATGGTGG+AGG + tig0024012:29599-29618 Msa1413130:CDS 55.0%
AACTTCGGACAGCGGACCAG+TGG + tig0024012:28506-28525 Msa1413130:intron 60.0%
GTCTTTGGTCCGAAGACCGG+AGG + tig0024012:28576-28595 Msa1413130:intron 60.0%
TTTGGTCCGAAGACCGGAGG+AGG + tig0024012:28579-28598 Msa1413130:intron 60.0%
TTGGTCCGAAGACCGGAGGA+GGG + tig0024012:28580-28599 Msa1413130:intron 60.0%
TGGTCCGAAGACCGGAGGAG+GGG + tig0024012:28581-28600 Msa1413130:intron 65.0%
ACACCCCCTCCTCCGGTCTT+CGG - tig0024012:28588-28607 None:intergenic 65.0%
GGTCCGAAGACCGGAGGAGG+GGG + tig0024012:28582-28601 Msa1413130:intron 70.0%
Chromosome Type Strat End Strand Name
tig0024012 gene 25433 30155 25433 ID=Msa1413130;Name=Msa1413130
tig0024012 mRNA 25433 30155 25433 ID=Msa1413130-mRNA-1;Parent=Msa1413130;Name=Msa1413130-mRNA-1;_AED=0.02;_eAED=0.02;_QI=192|1|1|1|1|1|2|221|405
tig0024012 exon 25433 26056 25433 ID=Msa1413130-mRNA-1:exon:20985;Parent=Msa1413130-mRNA-1
tig0024012 exon 29149 30155 29149 ID=Msa1413130-mRNA-1:exon:20986;Parent=Msa1413130-mRNA-1
tig0024012 five_prime_UTR 25433 25624 25433 ID=Msa1413130-mRNA-1:five_prime_utr;Parent=Msa1413130-mRNA-1
tig0024012 CDS 25625 26056 25625 ID=Msa1413130-mRNA-1:cds;Parent=Msa1413130-mRNA-1
tig0024012 CDS 29149 29934 29149 ID=Msa1413130-mRNA-1:cds;Parent=Msa1413130-mRNA-1
tig0024012 three_prime_UTR 29935 30155 29935 ID=Msa1413130-mRNA-1:three_prime_utr;Parent=Msa1413130-mRNA-1
Gene Sequence

>Msa1413130

ATGGAGTCATGTGAGGCAACAGAGATTTTCAAACCTAAACGGATTTTCTTGTTCCCACTCACAGTACTCATTCTTGTGATACTTCTTCCTCTTCTCATTCACTTCAATCAAACGTCATCAAAAGTACTATATCCAACAACAACTATTGTAGAACCTAGCTGTAACAACTTGTTCTTAGGAACATGGGTTCCTTATCTAAAACAACCTTATTACAATCAAACATGTCCTTTTATAACTGAAAAACAGAACTGTCTTATTCACGGAAGACCAGACAGTGATTTTCTTAAGTGGAGATGGAAACCTGATGATTGTGAACTTCCTTTGTTTGATGCAACTCAGTTTTTCAAGATTGTTAAGGGAAAGTCAATGGCTTTTGTCGGTGACTCTATTGGGAGAAATCAGATGGAATCATTACTATGTCTCTTGAATTCTGTTGCTCGTGCTGAGGATATTACAACTAAATATGTATCATCAATTGAAGACCTAACATATTTCAAATGGTGGTTTTATGCTGATTATAATTTTACCATTGCAATGCTATGGTCCCCTTTTCTAGTAAAATCCTCTAAATCTTACATCTACAACAGTTCTAATTTCTACAAACCCGAGAGTCTTTATCTAGATGAACCAGATACAGCTTGGACAAGTCGAATTGGAAATTTCGACTATGTGATTTTCTCCGGTGGACAATGGTTTTTCCGACCATTCACATTCTACGAAAATGGTACAATTGTTGGATGTCAAAAATGCAACAACTCAATAGAACTCAACTATTACGGATACAAGAAAGCGTATCGAACAGCTTTCAAAACTATTATGAATCCTAATCTGTTCAAAGGGTTGGCATTTTTGGTGACACATTCTCCAAATCATTTCGAGAATGGCGAATGGAACAATGGTGGAGGGTGTAATAGAACACAACCATTCAGTAACGAACATAAATGGGAGGTGCATCCATATGGTCTAGAGATTATACATCAAATACAATTGGATGAATTTAGTGCTGCAAAGAACAATACAGGGAAGAATGGTTCACGTTTTGGTTTGATTGATATAACAGATGCAATGTTGATGAGACCTGATGGACATCCTAACAAATATGGGCATGTGTCTGGTCAAAATGCAAGCATTAATGATTGTGTTCATTGGTGTATGCCAGGGCCTGTTGACACTATTAATGAGATTTTTCTCTATATGATCATGAGAATGGGATTGTGA
Protein sequence

>Msa1413130

MESCEATEIFKPKRIFLFPLTVLILVILLPLLIHFNQTSSKVLYPTTTIVEPSCNNLFLGTWVPYLKQPYYNQTCPFITEKQNCLIHGRPDSDFLKWRWKPDDCELPLFDATQFFKIVKGKSMAFVGDSIGRNQMESLLCLLNSVARAEDITTKYVSSIEDLTYFKWWFYADYNFTIAMLWSPFLVKSSKSYIYNSSNFYKPESLYLDEPDTAWTSRIGNFDYVIFSGGQWFFRPFTFYENGTIVGCQKCNNSIELNYYGYKKAYRTAFKTIMNPNLFKGLAFLVTHSPNHFENGEWNNGGGCNRTQPFSNEHKWEVHPYGLEIIHQIQLDEFSAAKNNTGKNGSRFGLIDITDAMLMRPDGHPNKYGHVSGQNASINDCVHWCMPGPVDTINEIFLYMIMRMGL*