AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0862740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862740 MtrunA17_Chr5g0444601 95.312 192 7 1 1 190 3 194 6.52e-128 357
Msa0862740 MtrunA17_Chr5g0444611 83.422 187 28 2 1 185 47 232 8.90e-89 260
Msa0862740 MtrunA17_Chr3g0099551 45.503 189 78 5 1 185 1 168 3.43e-33 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAGATTTATGGTGTTAA+TGG 0.238978 5_4:-62462669 Msa0862740:CDS
CCTTGTTACCTTCCTCTCTT+AGG 0.249740 5_4:-62463064 Msa0862740:CDS
GAAGTAGAAGATGAGATTTA+TGG 0.261780 5_4:-62462678 Msa0862740:CDS
TGTTCTAGCATTTCATGTTT+CGG 0.336351 5_4:+62462921 None:intergenic
AGATTTATTAACTTTGTTCA+TGG 0.336906 5_4:+62463133 None:intergenic
GCAAAAGCTGAAGCTTTCAT+TGG 0.375645 5_4:-62462633 Msa0862740:CDS
GAATGTTGAAGATTAAATAA+AGG 0.422149 5_4:+62463033 None:intergenic
AGATGCAGAGAGAGGGCTGC+TGG 0.439749 5_4:-62462590 Msa0862740:CDS
CCTAAGAGAGGAAGGTAACA+AGG 0.502209 5_4:+62463064 None:intergenic
AGAAGATGATTTAGAAGTGA+AGG 0.515399 5_4:-62462712 Msa0862740:CDS
TGATTTAGAAGTGAAGGATG+AGG 0.517126 5_4:-62462706 Msa0862740:CDS
AACATTCTTATCATTGCACT+TGG 0.519113 5_4:-62463017 Msa0862740:CDS
TGGTGTTAGTAAAGTGGATA+AGG 0.531732 5_4:-62462781 Msa0862740:CDS
AAGATTAAATAAAGGTAACT+AGG 0.532639 5_4:+62463041 None:intergenic
GATGCAGAGAGAGGGCTGCT+GGG 0.533020 5_4:-62462589 Msa0862740:CDS
ATTGCACTTGGAGCTGAAGC+CGG 0.547486 5_4:-62463005 Msa0862740:CDS
GTATGTTGGTGTTAGTAAAG+TGG 0.551450 5_4:-62462787 Msa0862740:CDS
TAACTAGGCCTAAGAGAGGA+AGG 0.551563 5_4:+62463056 None:intergenic
ACAAAATGAAAAGAAGCCTA+AGG 0.566232 5_4:-62462844 Msa0862740:CDS
CAGTTGAAGATGCAGAGAGA+GGG 0.576252 5_4:-62462597 Msa0862740:CDS
GTAACAAGGGTGTGATCACA+AGG 0.579955 5_4:+62463078 None:intergenic
AGAGAACACCGACAAAAGAC+CGG 0.585897 5_4:+62462986 None:intergenic
GCAGTTGAAGATGCAGAGAG+AGG 0.589013 5_4:-62462598 Msa0862740:CDS
GAGAAGAAGATTGTGTATGT+TGG 0.605699 5_4:-62462801 Msa0862740:CDS
AAGGTAACTAGGCCTAAGAG+AGG 0.633233 5_4:+62463052 None:intergenic
CTAAGAGAGGAAGGTAACAA+GGG 0.639907 5_4:+62463065 None:intergenic
AAATGAAAAGAAGCCTAAGG+TGG 0.649751 5_4:-62462841 Msa0862740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTCTATATAAAAAATACT+TGG + chr5_4:62462977-62462996 None:intergenic 15.0%
!! AAGATTAAATAAAGGTAACT+AGG + chr5_4:62462674-62462693 None:intergenic 20.0%
!! GAATGTTGAAGATTAAATAA+AGG + chr5_4:62462682-62462701 None:intergenic 20.0%
!! TAAAAAATACTTGGCATTGA+AGG + chr5_4:62462968-62462987 None:intergenic 25.0%
AACATTCTTATCATTGCACT+TGG - chr5_4:62462695-62462714 Msa0862740:CDS 30.0%
TGTTCTAGCATTTCATGTTT+CGG + chr5_4:62462794-62462813 None:intergenic 30.0%
ACAAAATGAAAAGAAGCCTA+AGG - chr5_4:62462868-62462887 Msa0862740:CDS 30.0%
AGAAGATGATTTAGAAGTGA+AGG - chr5_4:62463000-62463019 Msa0862740:CDS 30.0%
GAAGTAGAAGATGAGATTTA+TGG - chr5_4:62463034-62463053 Msa0862740:CDS 30.0%
! GATGAGATTTATGGTGTTAA+TGG - chr5_4:62463043-62463062 Msa0862740:CDS 30.0%
!!! TGTTGCTTTTTGTCTTCAGA+AGG + chr5_4:62462755-62462774 None:intergenic 35.0%
AAATGAAAAGAAGCCTAAGG+TGG - chr5_4:62462871-62462890 Msa0862740:CDS 35.0%
!! ACAGATTTTTCAACCACCTT+AGG + chr5_4:62462887-62462906 None:intergenic 35.0%
GAGAAGAAGATTGTGTATGT+TGG - chr5_4:62462911-62462930 Msa0862740:CDS 35.0%
!! GTATGTTGGTGTTAGTAAAG+TGG - chr5_4:62462925-62462944 Msa0862740:CDS 35.0%
! TGGTGTTAGTAAAGTGGATA+AGG - chr5_4:62462931-62462950 Msa0862740:CDS 35.0%
TGATTTAGAAGTGAAGGATG+AGG - chr5_4:62463006-62463025 Msa0862740:CDS 35.0%
CTAAGAGAGGAAGGTAACAA+GGG + chr5_4:62462650-62462669 None:intergenic 40.0%
GCAAAAGCTGAAGCTTTCAT+TGG - chr5_4:62463079-62463098 Msa0862740:CDS 40.0%
! GTAACAAGGGTGTGATCACA+AGG + chr5_4:62462637-62462656 None:intergenic 45.0%
CCTAAGAGAGGAAGGTAACA+AGG + chr5_4:62462651-62462670 None:intergenic 45.0%
CCTTGTTACCTTCCTCTCTT+AGG - chr5_4:62462648-62462667 Msa0862740:CDS 45.0%
TAACTAGGCCTAAGAGAGGA+AGG + chr5_4:62462659-62462678 None:intergenic 45.0%
AAGGTAACTAGGCCTAAGAG+AGG + chr5_4:62462663-62462682 None:intergenic 45.0%
AGAGAACACCGACAAAAGAC+CGG + chr5_4:62462729-62462748 None:intergenic 45.0%
CAGTTGAAGATGCAGAGAGA+GGG - chr5_4:62463115-62463134 Msa0862740:CDS 45.0%
ATTGCACTTGGAGCTGAAGC+CGG - chr5_4:62462707-62462726 Msa0862740:CDS 50.0%
! AGCTGAAGCCGGTCTTTTGT+CGG - chr5_4:62462718-62462737 Msa0862740:CDS 50.0%
GCAGTTGAAGATGCAGAGAG+AGG - chr5_4:62463114-62463133 Msa0862740:CDS 50.0%
AGATGCAGAGAGAGGGCTGC+TGG - chr5_4:62463122-62463141 Msa0862740:CDS 60.0%
GATGCAGAGAGAGGGCTGCT+GGG - chr5_4:62463123-62463142 Msa0862740:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 62462581 62463153 62462581 ID=Msa0862740;Name=Msa0862740
chr5_4 mRNA 62462581 62463153 62462581 ID=Msa0862740-mRNA-1;Parent=Msa0862740;Name=Msa0862740-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|190
chr5_4 exon 62462581 62463153 62462581 ID=Msa0862740-mRNA-1:exon:19509;Parent=Msa0862740-mRNA-1
chr5_4 CDS 62462581 62463153 62462581 ID=Msa0862740-mRNA-1:cds;Parent=Msa0862740-mRNA-1
Gene Sequence

>Msa0862740

ATGAACAAAGTTAATAAATCTCAAGTTATAGTTCTCTCTATCTTACTTCTTCTCCTTGTGATCACACCCTTGTTACCTTCCTCTCTTAGGCCTAGTTACCTTTATTTAATCTTCAACATTCTTATCATTGCACTTGGAGCTGAAGCCGGTCTTTTGTCGGTGTTCTCTGAACCTTCTGAAGACAAAAAGCAACATGTTTCTGTTACACAACCGAAACATGAAATGCTAGAACAAGAAGAGAAAGAAGCATCTAACATAATAAACAATGCATATTCTGTTTCAGAAGAACAAAATGAAAAGAAGCCTAAGGTGGTTGAAAAATCTGTATCTGAGAAGAAGATTGTGTATGTTGGTGTTAGTAAAGTGGATAAGGTGAAAAAGTGTCCTTCAATGCCAAGTATTTTTTATATAGAAGATGAAGAAGATGATTTAGAAGTGAAGGATGAGGAAGTTGAAGTAGAAGATGAGATTTATGGTGTTAATGGACAAGAACTTTTTGCAAAAGCTGAAGCTTTCATTGGAAACTTCTACAAGCAGTTGAAGATGCAGAGAGAGGGCTGCTGGGATTCTTAG

Protein sequence

>Msa0862740

MNKVNKSQVIVLSILLLLLVITPLLPSSLRPSYLYLIFNILIIALGAEAGLLSVFSEPSEDKKQHVSVTQPKHEMLEQEEKEASNIINNAYSVSEEQNEKKPKVVEKSVSEKKIVYVGVSKVDKVKKCPSMPSIFYIEDEEDDLEVKDEEVEVEDEIYGVNGQELFAKAEAFIGNFYKQLKMQREGCWDS*