AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0744790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATAATAACAGTGAAAC+TGG 0.302635 5_1:+65846067 None:intergenic
TGTGTTCTAGCAACTTGAAT+TGG 0.324721 5_1:+65846591 None:intergenic
GACTGCTAAGGGACATCAAA+TGG 0.354379 5_1:-65845952 Msa0744790:three_prime_UTR
AGCACTAACTCAGCATGTTC+AGG 0.362910 5_1:+65846531 None:intergenic
TGAATTGGAATTTGAGGAAA+AGG 0.370298 5_1:+65846606 None:intergenic
GCAACTTGAATTGGAATTTG+AGG 0.378140 5_1:+65846600 None:intergenic
TGTGGGATCAAGATATATAA+AGG 0.423750 5_1:-65846112 Msa0744790:exon
TCAAAATTACCTCTTGAAGC+TGG 0.465001 5_1:+65847735 None:intergenic
ACTCAGCATGTTCAGGTCCT+TGG 0.469300 5_1:+65846538 None:intergenic
GGAAAAGGATTCTGAGTTGA+TGG 0.475687 5_1:+65846621 None:intergenic
TTTAATCATATGACTGCTAA+GGG 0.486787 5_1:-65845963 Msa0744790:three_prime_UTR
AATAACAGTGAAACTGGATC+TGG 0.488205 5_1:+65846073 None:intergenic
ACTGCTAAGGGACATCAAAT+GGG 0.515323 5_1:-65845951 Msa0744790:three_prime_UTR
TAATGTATCTTGATCTATTG+TGG 0.528080 5_1:-65846130 Msa0744790:CDS
AATGTATCTTGATCTATTGT+GGG 0.535708 5_1:-65846129 Msa0744790:CDS
CAAACTATGAATAATGAAGC+TGG 0.552669 5_1:-65846651 Msa0744790:CDS
TGCTAGAACACAAGAAGTTG+AGG 0.602315 5_1:-65846580 Msa0744790:CDS
GAGGCTGCTGAATTAGTACA+TGG 0.624946 5_1:-65846561 Msa0744790:CDS
GCTGAATTAGTACATGGCCA+AGG 0.646206 5_1:-65846555 Msa0744790:CDS
GGGATCAAGATATATAAAGG+AGG 0.651640 5_1:-65846109 Msa0744790:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATCTTTTTCTGATATA+TGG - chr5_1:65845959-65845978 Msa0744790:three_prime_UTR 15.0%
!! AAAATGTAACAAACATAATT+TGG + chr5_1:65846367-65846386 None:intergenic 15.0%
!! AATATATGATTCTTTATCTT+TGG - chr5_1:65846603-65846622 Msa0744790:CDS 15.0%
!! TCTTTACTAATTATTATCAA+AGG + chr5_1:65846861-65846880 None:intergenic 15.0%
!!! AAATTTTTAAATAATGAAGC+TGG - chr5_1:65847313-65847332 Msa0744790:intron 15.0%
!!! ACTGTATTAAGTTTTTTATT+AGG - chr5_1:65847965-65847984 Msa0744790:intron 15.0%
!!! ATTAATCTTTTTCTGATATA+TGG - chr5_1:65845959-65845978 Msa0744790:three_prime_UTR 15.0%
!! AAAATGTAACAAACATAATT+TGG + chr5_1:65846367-65846386 None:intergenic 15.0%
!! AATATATGATTCTTTATCTT+TGG - chr5_1:65846603-65846622 Msa0744790:CDS 15.0%
!! TCTTTACTAATTATTATCAA+AGG + chr5_1:65846861-65846880 None:intergenic 15.0%
!!! AAATTTTTAAATAATGAAGC+TGG - chr5_1:65847313-65847332 Msa0744790:intron 15.0%
!!! ACTGTATTAAGTTTTTTATT+AGG - chr5_1:65847965-65847984 Msa0744790:intron 15.0%
!! ACAAACATAATTTGGATAAA+AGG + chr5_1:65846359-65846378 None:intergenic 20.0%
!!! AGTTCTAACATTTATAGTAA+TGG - chr5_1:65846419-65846438 Msa0744790:intron 20.0%
!! TGGAGAATAGATAAAATATA+AGG - chr5_1:65846721-65846740 Msa0744790:intron 20.0%
!!! ATGAAGGTATCTTTAATAAA+AGG + chr5_1:65846834-65846853 None:intergenic 20.0%
!! TATTATCAAAGGTTAAATGA+AGG + chr5_1:65846850-65846869 None:intergenic 20.0%
!! AATATCAAAAGATAAGTTAG+CGG + chr5_1:65846908-65846927 None:intergenic 20.0%
!! AATTTATTAGGTGAGTATTT+AGG + chr5_1:65847298-65847317 None:intergenic 20.0%
!!! AAAAACTACCTTTATCTTTT+TGG + chr5_1:65847658-65847677 None:intergenic 20.0%
!!! TGCTATATTGTTATATTTTG+TGG - chr5_1:65847782-65847801 Msa0744790:intron 20.0%
!! ACAAACATAATTTGGATAAA+AGG + chr5_1:65846359-65846378 None:intergenic 20.0%
!!! AGTTCTAACATTTATAGTAA+TGG - chr5_1:65846419-65846438 Msa0744790:intron 20.0%
!! TGGAGAATAGATAAAATATA+AGG - chr5_1:65846721-65846740 Msa0744790:intron 20.0%
!!! ATGAAGGTATCTTTAATAAA+AGG + chr5_1:65846834-65846853 None:intergenic 20.0%
!! TATTATCAAAGGTTAAATGA+AGG + chr5_1:65846850-65846869 None:intergenic 20.0%
!! AATATCAAAAGATAAGTTAG+CGG + chr5_1:65846908-65846927 None:intergenic 20.0%
!! AATTTATTAGGTGAGTATTT+AGG + chr5_1:65847298-65847317 None:intergenic 20.0%
!!! AAAAACTACCTTTATCTTTT+TGG + chr5_1:65847658-65847677 None:intergenic 20.0%
!!! TGCTATATTGTTATATTTTG+TGG - chr5_1:65847782-65847801 Msa0744790:intron 20.0%
!! TTTTCTCTTTTTATCAGCTT+TGG - chr5_1:65846218-65846237 Msa0744790:intron 25.0%
!!! ATTCTTTATCTTTGGAGTTT+TGG - chr5_1:65846611-65846630 Msa0744790:CDS 25.0%
!! ATTTTATCTATTCTCCAAAG+TGG + chr5_1:65846718-65846737 None:intergenic 25.0%
!! GTTTGTTACATTAATTGCTA+TGG - chr5_1:65846781-65846800 Msa0744790:intron 25.0%
! TACAATATACACAAATGACA+AGG + chr5_1:65846995-65847014 None:intergenic 25.0%
!! TGTCATTTGTGTATATTGTA+TGG - chr5_1:65846995-65847014 Msa0744790:intron 25.0%
! AGGACTGTAATAATGTTTAA+GGG + chr5_1:65847278-65847297 None:intergenic 25.0%
! TAGGACTGTAATAATGTTTA+AGG + chr5_1:65847279-65847298 None:intergenic 25.0%
! TGATATGTCCAAAAAGATAA+AGG - chr5_1:65847647-65847666 Msa0744790:intron 25.0%
!!! ATTTTCTGTATTTTTTGCAC+AGG - chr5_1:65847832-65847851 Msa0744790:intron 25.0%
! TAATGTATCTTGATCTATTG+TGG - chr5_1:65847873-65847892 Msa0744790:intron 25.0%
! AATGTATCTTGATCTATTGT+GGG - chr5_1:65847874-65847893 Msa0744790:intron 25.0%
! GAAAATAATAACAGTGAAAC+TGG + chr5_1:65847939-65847958 None:intergenic 25.0%
!! TTTTAATCATATGACTGCTA+AGG - chr5_1:65848039-65848058 Msa0744790:intron 25.0%
! TTTAATCATATGACTGCTAA+GGG - chr5_1:65848040-65848059 Msa0744790:intron 25.0%
!! TTTTCTCTTTTTATCAGCTT+TGG - chr5_1:65846218-65846237 Msa0744790:intron 25.0%
!!! ATTCTTTATCTTTGGAGTTT+TGG - chr5_1:65846611-65846630 Msa0744790:CDS 25.0%
!! ATTTTATCTATTCTCCAAAG+TGG + chr5_1:65846718-65846737 None:intergenic 25.0%
!! GTTTGTTACATTAATTGCTA+TGG - chr5_1:65846781-65846800 Msa0744790:intron 25.0%
! TACAATATACACAAATGACA+AGG + chr5_1:65846995-65847014 None:intergenic 25.0%
!! TGTCATTTGTGTATATTGTA+TGG - chr5_1:65846995-65847014 Msa0744790:intron 25.0%
! AGGACTGTAATAATGTTTAA+GGG + chr5_1:65847278-65847297 None:intergenic 25.0%
! TAGGACTGTAATAATGTTTA+AGG + chr5_1:65847279-65847298 None:intergenic 25.0%
! TGATATGTCCAAAAAGATAA+AGG - chr5_1:65847647-65847666 Msa0744790:intron 25.0%
!!! ATTTTCTGTATTTTTTGCAC+AGG - chr5_1:65847832-65847851 Msa0744790:intron 25.0%
! TAATGTATCTTGATCTATTG+TGG - chr5_1:65847873-65847892 Msa0744790:intron 25.0%
! AATGTATCTTGATCTATTGT+GGG - chr5_1:65847874-65847893 Msa0744790:intron 25.0%
! GAAAATAATAACAGTGAAAC+TGG + chr5_1:65847939-65847958 None:intergenic 25.0%
!! TTTTAATCATATGACTGCTA+AGG - chr5_1:65848039-65848058 Msa0744790:intron 25.0%
! TTTAATCATATGACTGCTAA+GGG - chr5_1:65848040-65848059 Msa0744790:intron 25.0%
CATAATTTGGATAAAAGGAC+TGG + chr5_1:65846354-65846373 None:intergenic 30.0%
ATATATATGTGTGTCTGTCT+AGG - chr5_1:65846467-65846486 Msa0744790:intron 30.0%
GGAACTAAGGAAGAAAAATT+AGG + chr5_1:65846522-65846541 None:intergenic 30.0%
!! ATTTGAGTTCTAAGGAACTA+AGG + chr5_1:65846535-65846554 None:intergenic 30.0%
! TAGAGATCATTTGAGTTCTA+AGG + chr5_1:65846543-65846562 None:intergenic 30.0%
! CTTTAATAAAAGGTTGACTC+AGG + chr5_1:65846824-65846843 None:intergenic 30.0%
TCTAGAAAAGATGAATCAAG+AGG - chr5_1:65846931-65846950 Msa0744790:intron 30.0%
! TGCATGATTTTTAATGCCTA+TGG - chr5_1:65847087-65847106 Msa0744790:intron 30.0%
ATAAAAAACTCAGCAGACAT+AGG + chr5_1:65847197-65847216 None:intergenic 30.0%
CAAACTATGAATAATGAAGC+TGG - chr5_1:65847352-65847371 Msa0744790:intron 30.0%
TGAATTGGAATTTGAGGAAA+AGG + chr5_1:65847400-65847419 None:intergenic 30.0%
AGTGAAACAGAAGTTACAAA+AGG + chr5_1:65847572-65847591 None:intergenic 30.0%
TGTGGGATCAAGATATATAA+AGG - chr5_1:65847891-65847910 Msa0744790:intron 30.0%
CATAATTTGGATAAAAGGAC+TGG + chr5_1:65846354-65846373 None:intergenic 30.0%
ATATATATGTGTGTCTGTCT+AGG - chr5_1:65846467-65846486 Msa0744790:intron 30.0%
GGAACTAAGGAAGAAAAATT+AGG + chr5_1:65846522-65846541 None:intergenic 30.0%
!! ATTTGAGTTCTAAGGAACTA+AGG + chr5_1:65846535-65846554 None:intergenic 30.0%
! TAGAGATCATTTGAGTTCTA+AGG + chr5_1:65846543-65846562 None:intergenic 30.0%
! CTTTAATAAAAGGTTGACTC+AGG + chr5_1:65846824-65846843 None:intergenic 30.0%
TCTAGAAAAGATGAATCAAG+AGG - chr5_1:65846931-65846950 Msa0744790:intron 30.0%
! TGCATGATTTTTAATGCCTA+TGG - chr5_1:65847087-65847106 Msa0744790:intron 30.0%
ATAAAAAACTCAGCAGACAT+AGG + chr5_1:65847197-65847216 None:intergenic 30.0%
CAAACTATGAATAATGAAGC+TGG - chr5_1:65847352-65847371 Msa0744790:intron 30.0%
TGAATTGGAATTTGAGGAAA+AGG + chr5_1:65847400-65847419 None:intergenic 30.0%
AGTGAAACAGAAGTTACAAA+AGG + chr5_1:65847572-65847591 None:intergenic 30.0%
TGTGGGATCAAGATATATAA+AGG - chr5_1:65847891-65847910 Msa0744790:intron 30.0%
! AAGCTTCAAGTTAGAATGTG+TGG - chr5_1:65846148-65846167 Msa0744790:CDS 35.0%
! AGCTTCAAGTTAGAATGTGT+GGG - chr5_1:65846149-65846168 Msa0744790:CDS 35.0%
TCAAAATTACCTCTTGAAGC+TGG + chr5_1:65846271-65846290 None:intergenic 35.0%
!! TTCTGAGGTTCTAGTTATAG+AGG - chr5_1:65846640-65846659 Msa0744790:CDS 35.0%
! TCTGAGGTTCTAGTTATAGA+GGG - chr5_1:65846641-65846660 Msa0744790:CDS 35.0%
TGCATAGAAATTGACCACTT+TGG - chr5_1:65846701-65846720 Msa0744790:intron 35.0%
GCAACTTGAATTGGAATTTG+AGG + chr5_1:65847406-65847425 None:intergenic 35.0%
TGTGTTCTAGCAACTTGAAT+TGG + chr5_1:65847415-65847434 None:intergenic 35.0%
TAAGCATGTTGTTAGTTCGA+TGG + chr5_1:65847618-65847637 None:intergenic 35.0%
GGGATCAAGATATATAAAGG+AGG - chr5_1:65847894-65847913 Msa0744790:intron 35.0%
AATAACAGTGAAACTGGATC+TGG + chr5_1:65847933-65847952 None:intergenic 35.0%
! AAGCTTCAAGTTAGAATGTG+TGG - chr5_1:65846148-65846167 Msa0744790:CDS 35.0%
! AGCTTCAAGTTAGAATGTGT+GGG - chr5_1:65846149-65846168 Msa0744790:CDS 35.0%
TCAAAATTACCTCTTGAAGC+TGG + chr5_1:65846271-65846290 None:intergenic 35.0%
!! TTCTGAGGTTCTAGTTATAG+AGG - chr5_1:65846640-65846659 Msa0744790:CDS 35.0%
! TCTGAGGTTCTAGTTATAGA+GGG - chr5_1:65846641-65846660 Msa0744790:CDS 35.0%
TGCATAGAAATTGACCACTT+TGG - chr5_1:65846701-65846720 Msa0744790:intron 35.0%
GCAACTTGAATTGGAATTTG+AGG + chr5_1:65847406-65847425 None:intergenic 35.0%
TGTGTTCTAGCAACTTGAAT+TGG + chr5_1:65847415-65847434 None:intergenic 35.0%
TAAGCATGTTGTTAGTTCGA+TGG + chr5_1:65847618-65847637 None:intergenic 35.0%
GGGATCAAGATATATAAAGG+AGG - chr5_1:65847894-65847913 Msa0744790:intron 35.0%
AATAACAGTGAAACTGGATC+TGG + chr5_1:65847933-65847952 None:intergenic 35.0%
! GCAAATTTTCCAGCTTCAAG+AGG - chr5_1:65846259-65846278 Msa0744790:intron 40.0%
AATCAAGAGGCTAATGAGCA+AGG - chr5_1:65846944-65846963 Msa0744790:intron 40.0%
ACTAAGTGTGCATAGAGACA+TGG + chr5_1:65847150-65847169 None:intergenic 40.0%
! GGAAAAGGATTCTGAGTTGA+TGG + chr5_1:65847385-65847404 None:intergenic 40.0%
TGCTAGAACACAAGAAGTTG+AGG - chr5_1:65847423-65847442 Msa0744790:intron 40.0%
AAATGTCTCCAAGCCATTGA+AGG + chr5_1:65847529-65847548 None:intergenic 40.0%
ACTGCTAAGGGACATCAAAT+GGG - chr5_1:65848052-65848071 Msa0744790:intron 40.0%
! GCAAATTTTCCAGCTTCAAG+AGG - chr5_1:65846259-65846278 Msa0744790:intron 40.0%
AATCAAGAGGCTAATGAGCA+AGG - chr5_1:65846944-65846963 Msa0744790:intron 40.0%
ACTAAGTGTGCATAGAGACA+TGG + chr5_1:65847150-65847169 None:intergenic 40.0%
! GGAAAAGGATTCTGAGTTGA+TGG + chr5_1:65847385-65847404 None:intergenic 40.0%
TGCTAGAACACAAGAAGTTG+AGG - chr5_1:65847423-65847442 Msa0744790:intron 40.0%
AAATGTCTCCAAGCCATTGA+AGG + chr5_1:65847529-65847548 None:intergenic 40.0%
!! GAGTTTTGGCAGTAGTTCTG+AGG - chr5_1:65846625-65846644 Msa0744790:CDS 45.0%
ATAAACGACTCAGCAGCCAT+AGG + chr5_1:65847106-65847125 None:intergenic 45.0%
GAGGCTGCTGAATTAGTACA+TGG - chr5_1:65847442-65847461 Msa0744790:intron 45.0%
GCTGAATTAGTACATGGCCA+AGG - chr5_1:65847448-65847467 Msa0744790:intron 45.0%
AGCACTAACTCAGCATGTTC+AGG + chr5_1:65847475-65847494 None:intergenic 45.0%
CCATTGAAGGATGTCTAGCA+TGG + chr5_1:65847516-65847535 None:intergenic 45.0%
CCATGCTAGACATCCTTCAA+TGG - chr5_1:65847513-65847532 Msa0744790:intron 45.0%
CTAGACATCCTTCAATGGCT+TGG - chr5_1:65847518-65847537 Msa0744790:intron 45.0%
GACTGCTAAGGGACATCAAA+TGG - chr5_1:65848051-65848070 Msa0744790:intron 45.0%
!! GAGTTTTGGCAGTAGTTCTG+AGG - chr5_1:65846625-65846644 Msa0744790:CDS 45.0%
ATAAACGACTCAGCAGCCAT+AGG + chr5_1:65847106-65847125 None:intergenic 45.0%
GAGGCTGCTGAATTAGTACA+TGG - chr5_1:65847442-65847461 Msa0744790:intron 45.0%
GCTGAATTAGTACATGGCCA+AGG - chr5_1:65847448-65847467 Msa0744790:intron 45.0%
AGCACTAACTCAGCATGTTC+AGG + chr5_1:65847475-65847494 None:intergenic 45.0%
CCATTGAAGGATGTCTAGCA+TGG + chr5_1:65847516-65847535 None:intergenic 45.0%
CCATGCTAGACATCCTTCAA+TGG - chr5_1:65847513-65847532 Msa0744790:intron 45.0%
CTAGACATCCTTCAATGGCT+TGG - chr5_1:65847518-65847537 Msa0744790:intron 45.0%
GACTGCTAAGGGACATCAAA+TGG - chr5_1:65848051-65848070 Msa0744790:intron 45.0%
!! TCATTATTTAAAAATTTATT+AGG + chr5_1:65847310-65847329 None:intergenic 5.0%
!!! ATTTTTAAACTTATTTTTAT+TGG - chr5_1:65847997-65848016 Msa0744790:intron 5.0%
!! TCATTATTTAAAAATTTATT+AGG + chr5_1:65847310-65847329 None:intergenic 5.0%
!!! ATTTTTAAACTTATTTTTAT+TGG - chr5_1:65847997-65848016 Msa0744790:intron 5.0%
ACTCAGCATGTTCAGGTCCT+TGG + chr5_1:65847468-65847487 None:intergenic 50.0%
ACTCAGCATGTTCAGGTCCT+TGG + chr5_1:65847468-65847487 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 65845951 65848074 65845951 ID=Msa0744790;Name=Msa0744790
chr5_1 mRNA 65845951 65848074 65845951 ID=Msa0744790-mRNA-1;Parent=Msa0744790;Name=Msa0744790-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0.25|0.25|0|0.5|4|164|88
chr5_1 exon 65845951 65846171 65845951 ID=Msa0744790-mRNA-1:exon:19583;Parent=Msa0744790-mRNA-1
chr5_1 exon 65846536 65846693 65846536 ID=Msa0744790-mRNA-1:exon:19582;Parent=Msa0744790-mRNA-1
chr5_1 exon 65847745 65847790 65847745 ID=Msa0744790-mRNA-1:exon:19581;Parent=Msa0744790-mRNA-1
chr5_1 exon 65848069 65848074 65848069 ID=Msa0744790-mRNA-1:exon:19580;Parent=Msa0744790-mRNA-1
chr5_1 CDS 65848069 65848074 65848069 ID=Msa0744790-mRNA-1:cds;Parent=Msa0744790-mRNA-1
chr5_1 CDS 65847745 65847790 65847745 ID=Msa0744790-mRNA-1:cds;Parent=Msa0744790-mRNA-1
chr5_1 CDS 65846536 65846693 65846536 ID=Msa0744790-mRNA-1:cds;Parent=Msa0744790-mRNA-1
chr5_1 CDS 65846115 65846171 65846115 ID=Msa0744790-mRNA-1:cds;Parent=Msa0744790-mRNA-1
chr5_1 three_prime_UTR 65845951 65846114 65845951 ID=Msa0744790-mRNA-1:three_prime_utr;Parent=Msa0744790-mRNA-1
Gene Sequence

>Msa0744790

AGTTTTCTTTGGAAACTTCTATCTTTTTCTGCAAATTTTCCAGCTTCAAGAGCTGGTGCAGCAGCATCAACTCAAACTATGAATAATGAAGCTGGTGCAGCACCATCAACTCAGAATCCTTTTCCTCAAATTCCAATTCAAGTTGCTAGAACACAAGAAGTTGAGGCTGCTGAATTAGTACATGGCCAAGGACCTGAACATGCTGAGTTAGTTAACAATATTAATGTGATAATGTATCTTGATCTATTGTGGGATCAAGATATATAA

Protein sequence

>Msa0744790

SFLWKLLSFSANFPASRAGAAASTQTMNNEAGAAPSTQNPFPQIPIQVARTQEVEAAELVHGQGPEHAELVNNINVIMYLDLLWDQDI*