AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATAATAACAGTGAAAC+TGG 0.302635 8_3:+60592339 None:intergenic
TGTGTTCTAGCAACTTGAAT+TGG 0.324721 8_3:+60592863 None:intergenic
GACTGCTAAGGGACATCAAA+TGG 0.354379 8_3:-60592224 Msa1299420:three_prime_UTR
AGCACTAACTCAGCATGTTC+AGG 0.362910 8_3:+60592803 None:intergenic
TGAATTGGAATTTGAGGAAA+AGG 0.370298 8_3:+60592878 None:intergenic
GCAACTTGAATTGGAATTTG+AGG 0.378140 8_3:+60592872 None:intergenic
TGTGGGATCAAGATATATAA+AGG 0.423750 8_3:-60592384 Msa1299420:exon
TCAAAATTACCTCTTGAAGC+TGG 0.465001 8_3:+60594007 None:intergenic
ACTCAGCATGTTCAGGTCCT+TGG 0.469300 8_3:+60592810 None:intergenic
GGAAAAGGATTCTGAGTTGA+TGG 0.475687 8_3:+60592893 None:intergenic
TTTAATCATATGACTGCTAA+GGG 0.486787 8_3:-60592235 Msa1299420:three_prime_UTR
AATAACAGTGAAACTGGATC+TGG 0.488205 8_3:+60592345 None:intergenic
ACTGCTAAGGGACATCAAAT+GGG 0.515323 8_3:-60592223 Msa1299420:three_prime_UTR
TAATGTATCTTGATCTATTG+TGG 0.528080 8_3:-60592402 Msa1299420:CDS
AATGTATCTTGATCTATTGT+GGG 0.535708 8_3:-60592401 Msa1299420:CDS
CAAACTATGAATAATGAAGC+TGG 0.552669 8_3:-60592923 Msa1299420:CDS
TGCTAGAACACAAGAAGTTG+AGG 0.602315 8_3:-60592852 Msa1299420:CDS
GAGGCTGCTGAATTAGTACA+TGG 0.624946 8_3:-60592833 Msa1299420:CDS
GCTGAATTAGTACATGGCCA+AGG 0.646206 8_3:-60592827 Msa1299420:CDS
GGGATCAAGATATATAAAGG+AGG 0.651640 8_3:-60592381 Msa1299420:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATCTTTTTCTGATATA+TGG - chr8_3:60592231-60592250 Msa1299420:three_prime_UTR 15.0%
!! AAAATGTAACAAACATAATT+TGG + chr8_3:60592639-60592658 None:intergenic 15.0%
!! AATATATGATTCTTTATCTT+TGG - chr8_3:60592875-60592894 Msa1299420:CDS 15.0%
!! TCTTTACTAATTATTATCAA+AGG + chr8_3:60593133-60593152 None:intergenic 15.0%
!!! AAATTTTTAAATAATGAAGC+TGG - chr8_3:60593585-60593604 Msa1299420:intron 15.0%
!!! ACTGTATTAAGTTTTTTATT+AGG - chr8_3:60594237-60594256 Msa1299420:intron 15.0%
!!! ATTAATCTTTTTCTGATATA+TGG - chr8_3:60592231-60592250 Msa1299420:three_prime_UTR 15.0%
!! AAAATGTAACAAACATAATT+TGG + chr8_3:60592639-60592658 None:intergenic 15.0%
!! AATATATGATTCTTTATCTT+TGG - chr8_3:60592875-60592894 Msa1299420:CDS 15.0%
!! TCTTTACTAATTATTATCAA+AGG + chr8_3:60593133-60593152 None:intergenic 15.0%
!!! AAATTTTTAAATAATGAAGC+TGG - chr8_3:60593585-60593604 Msa1299420:intron 15.0%
!!! ACTGTATTAAGTTTTTTATT+AGG - chr8_3:60594237-60594256 Msa1299420:intron 15.0%
!! ACAAACATAATTTGGATAAA+AGG + chr8_3:60592631-60592650 None:intergenic 20.0%
!!! AGTTCTAACATTTATAGTAA+TGG - chr8_3:60592691-60592710 Msa1299420:intron 20.0%
!! TGGAGAATAGATAAAATATA+AGG - chr8_3:60592993-60593012 Msa1299420:intron 20.0%
!!! ATGAAGGTATCTTTAATAAA+AGG + chr8_3:60593106-60593125 None:intergenic 20.0%
!! TATTATCAAAGGTTAAATGA+AGG + chr8_3:60593122-60593141 None:intergenic 20.0%
!! AATATCAAAAGATAAGTTAG+CGG + chr8_3:60593180-60593199 None:intergenic 20.0%
!! AATTTATTAGGTGAGTATTT+AGG + chr8_3:60593570-60593589 None:intergenic 20.0%
!!! AAAAACTACCTTTATCTTTT+TGG + chr8_3:60593930-60593949 None:intergenic 20.0%
!!! TGCTATATTGTTATATTTTG+TGG - chr8_3:60594054-60594073 Msa1299420:intron 20.0%
!! ACAAACATAATTTGGATAAA+AGG + chr8_3:60592631-60592650 None:intergenic 20.0%
!!! AGTTCTAACATTTATAGTAA+TGG - chr8_3:60592691-60592710 Msa1299420:intron 20.0%
!! TGGAGAATAGATAAAATATA+AGG - chr8_3:60592993-60593012 Msa1299420:intron 20.0%
!!! ATGAAGGTATCTTTAATAAA+AGG + chr8_3:60593106-60593125 None:intergenic 20.0%
!! TATTATCAAAGGTTAAATGA+AGG + chr8_3:60593122-60593141 None:intergenic 20.0%
!! AATATCAAAAGATAAGTTAG+CGG + chr8_3:60593180-60593199 None:intergenic 20.0%
!! AATTTATTAGGTGAGTATTT+AGG + chr8_3:60593570-60593589 None:intergenic 20.0%
!!! AAAAACTACCTTTATCTTTT+TGG + chr8_3:60593930-60593949 None:intergenic 20.0%
!!! TGCTATATTGTTATATTTTG+TGG - chr8_3:60594054-60594073 Msa1299420:intron 20.0%
!! TTTTCTCTTTTTATCAGCTT+TGG - chr8_3:60592490-60592509 Msa1299420:intron 25.0%
!!! ATTCTTTATCTTTGGAGTTT+TGG - chr8_3:60592883-60592902 Msa1299420:CDS 25.0%
!! ATTTTATCTATTCTCCAAAG+TGG + chr8_3:60592990-60593009 None:intergenic 25.0%
!! GTTTGTTACATTAATTGCTA+TGG - chr8_3:60593053-60593072 Msa1299420:intron 25.0%
! TACAATATACACAAATGACA+AGG + chr8_3:60593267-60593286 None:intergenic 25.0%
!! TGTCATTTGTGTATATTGTA+TGG - chr8_3:60593267-60593286 Msa1299420:intron 25.0%
! AGGACTGTAATAATGTTTAA+GGG + chr8_3:60593550-60593569 None:intergenic 25.0%
! TAGGACTGTAATAATGTTTA+AGG + chr8_3:60593551-60593570 None:intergenic 25.0%
! TGATATGTCCAAAAAGATAA+AGG - chr8_3:60593919-60593938 Msa1299420:intron 25.0%
!!! ATTTTCTGTATTTTTTGCAC+AGG - chr8_3:60594104-60594123 Msa1299420:intron 25.0%
! TAATGTATCTTGATCTATTG+TGG - chr8_3:60594145-60594164 Msa1299420:intron 25.0%
! AATGTATCTTGATCTATTGT+GGG - chr8_3:60594146-60594165 Msa1299420:intron 25.0%
! GAAAATAATAACAGTGAAAC+TGG + chr8_3:60594211-60594230 None:intergenic 25.0%
!! TTTTAATCATATGACTGCTA+AGG - chr8_3:60594311-60594330 Msa1299420:intron 25.0%
! TTTAATCATATGACTGCTAA+GGG - chr8_3:60594312-60594331 Msa1299420:intron 25.0%
!! TTTTCTCTTTTTATCAGCTT+TGG - chr8_3:60592490-60592509 Msa1299420:intron 25.0%
!!! ATTCTTTATCTTTGGAGTTT+TGG - chr8_3:60592883-60592902 Msa1299420:CDS 25.0%
!! ATTTTATCTATTCTCCAAAG+TGG + chr8_3:60592990-60593009 None:intergenic 25.0%
!! GTTTGTTACATTAATTGCTA+TGG - chr8_3:60593053-60593072 Msa1299420:intron 25.0%
! TACAATATACACAAATGACA+AGG + chr8_3:60593267-60593286 None:intergenic 25.0%
!! TGTCATTTGTGTATATTGTA+TGG - chr8_3:60593267-60593286 Msa1299420:intron 25.0%
! AGGACTGTAATAATGTTTAA+GGG + chr8_3:60593550-60593569 None:intergenic 25.0%
! TAGGACTGTAATAATGTTTA+AGG + chr8_3:60593551-60593570 None:intergenic 25.0%
! TGATATGTCCAAAAAGATAA+AGG - chr8_3:60593919-60593938 Msa1299420:intron 25.0%
!!! ATTTTCTGTATTTTTTGCAC+AGG - chr8_3:60594104-60594123 Msa1299420:intron 25.0%
! TAATGTATCTTGATCTATTG+TGG - chr8_3:60594145-60594164 Msa1299420:intron 25.0%
! AATGTATCTTGATCTATTGT+GGG - chr8_3:60594146-60594165 Msa1299420:intron 25.0%
! GAAAATAATAACAGTGAAAC+TGG + chr8_3:60594211-60594230 None:intergenic 25.0%
!! TTTTAATCATATGACTGCTA+AGG - chr8_3:60594311-60594330 Msa1299420:intron 25.0%
! TTTAATCATATGACTGCTAA+GGG - chr8_3:60594312-60594331 Msa1299420:intron 25.0%
CATAATTTGGATAAAAGGAC+TGG + chr8_3:60592626-60592645 None:intergenic 30.0%
ATATATATGTGTGTCTGTCT+AGG - chr8_3:60592739-60592758 Msa1299420:intron 30.0%
GGAACTAAGGAAGAAAAATT+AGG + chr8_3:60592794-60592813 None:intergenic 30.0%
!! ATTTGAGTTCTAAGGAACTA+AGG + chr8_3:60592807-60592826 None:intergenic 30.0%
! TAGAGATCATTTGAGTTCTA+AGG + chr8_3:60592815-60592834 None:intergenic 30.0%
! CTTTAATAAAAGGTTGACTC+AGG + chr8_3:60593096-60593115 None:intergenic 30.0%
TCTAGAAAAGATGAATCAAG+AGG - chr8_3:60593203-60593222 Msa1299420:intron 30.0%
! TGCATGATTTTTAATGCCAA+TGG - chr8_3:60593359-60593378 Msa1299420:intron 30.0%
ATAAAAAACTCAGCAGACAT+AGG + chr8_3:60593469-60593488 None:intergenic 30.0%
CAAACTATGAATAATGAAGC+TGG - chr8_3:60593624-60593643 Msa1299420:intron 30.0%
TGAATTGGAATTTGAGGAAA+AGG + chr8_3:60593672-60593691 None:intergenic 30.0%
AGTGAAACAGAAGTTACAAA+AGG + chr8_3:60593844-60593863 None:intergenic 30.0%
TGTGGGATCAAGATATATAA+AGG - chr8_3:60594163-60594182 Msa1299420:intron 30.0%
CATAATTTGGATAAAAGGAC+TGG + chr8_3:60592626-60592645 None:intergenic 30.0%
ATATATATGTGTGTCTGTCT+AGG - chr8_3:60592739-60592758 Msa1299420:intron 30.0%
GGAACTAAGGAAGAAAAATT+AGG + chr8_3:60592794-60592813 None:intergenic 30.0%
!! ATTTGAGTTCTAAGGAACTA+AGG + chr8_3:60592807-60592826 None:intergenic 30.0%
! TAGAGATCATTTGAGTTCTA+AGG + chr8_3:60592815-60592834 None:intergenic 30.0%
! CTTTAATAAAAGGTTGACTC+AGG + chr8_3:60593096-60593115 None:intergenic 30.0%
TCTAGAAAAGATGAATCAAG+AGG - chr8_3:60593203-60593222 Msa1299420:intron 30.0%
! TGCATGATTTTTAATGCCAA+TGG - chr8_3:60593359-60593378 Msa1299420:intron 30.0%
ATAAAAAACTCAGCAGACAT+AGG + chr8_3:60593469-60593488 None:intergenic 30.0%
CAAACTATGAATAATGAAGC+TGG - chr8_3:60593624-60593643 Msa1299420:intron 30.0%
TGAATTGGAATTTGAGGAAA+AGG + chr8_3:60593672-60593691 None:intergenic 30.0%
AGTGAAACAGAAGTTACAAA+AGG + chr8_3:60593844-60593863 None:intergenic 30.0%
TGTGGGATCAAGATATATAA+AGG - chr8_3:60594163-60594182 Msa1299420:intron 30.0%
! AAGCTTCAAGTTAGAATGTG+TGG - chr8_3:60592420-60592439 Msa1299420:CDS 35.0%
! AGCTTCAAGTTAGAATGTGT+GGG - chr8_3:60592421-60592440 Msa1299420:CDS 35.0%
TCAAAATTACCTCTTGAAGC+TGG + chr8_3:60592543-60592562 None:intergenic 35.0%
!! TTCTGAGGTTCTAGTTATAG+AGG - chr8_3:60592912-60592931 Msa1299420:CDS 35.0%
! TCTGAGGTTCTAGTTATAGA+GGG - chr8_3:60592913-60592932 Msa1299420:CDS 35.0%
TGCATAGAAATTGACCACTT+TGG - chr8_3:60592973-60592992 Msa1299420:intron 35.0%
GCAACTTGAATTGGAATTTG+AGG + chr8_3:60593678-60593697 None:intergenic 35.0%
TGTGTTCTAGCAACTTGAAT+TGG + chr8_3:60593687-60593706 None:intergenic 35.0%
TAAGCATGTTGTTAGTTCGA+TGG + chr8_3:60593890-60593909 None:intergenic 35.0%
GGGATCAAGATATATAAAGG+AGG - chr8_3:60594166-60594185 Msa1299420:intron 35.0%
AATAACAGTGAAACTGGATC+TGG + chr8_3:60594205-60594224 None:intergenic 35.0%
! AAGCTTCAAGTTAGAATGTG+TGG - chr8_3:60592420-60592439 Msa1299420:CDS 35.0%
! AGCTTCAAGTTAGAATGTGT+GGG - chr8_3:60592421-60592440 Msa1299420:CDS 35.0%
TCAAAATTACCTCTTGAAGC+TGG + chr8_3:60592543-60592562 None:intergenic 35.0%
!! TTCTGAGGTTCTAGTTATAG+AGG - chr8_3:60592912-60592931 Msa1299420:CDS 35.0%
! TCTGAGGTTCTAGTTATAGA+GGG - chr8_3:60592913-60592932 Msa1299420:CDS 35.0%
TGCATAGAAATTGACCACTT+TGG - chr8_3:60592973-60592992 Msa1299420:intron 35.0%
GCAACTTGAATTGGAATTTG+AGG + chr8_3:60593678-60593697 None:intergenic 35.0%
TGTGTTCTAGCAACTTGAAT+TGG + chr8_3:60593687-60593706 None:intergenic 35.0%
TAAGCATGTTGTTAGTTCGA+TGG + chr8_3:60593890-60593909 None:intergenic 35.0%
GGGATCAAGATATATAAAGG+AGG - chr8_3:60594166-60594185 Msa1299420:intron 35.0%
AATAACAGTGAAACTGGATC+TGG + chr8_3:60594205-60594224 None:intergenic 35.0%
! GCAAATTTTCCAGCTTCAAG+AGG - chr8_3:60592531-60592550 Msa1299420:intron 40.0%
AATCAAGAGGCTAATGAGCA+AGG - chr8_3:60593216-60593235 Msa1299420:intron 40.0%
ACTAAGTGTGCATAGAGACA+TGG + chr8_3:60593422-60593441 None:intergenic 40.0%
! GGAAAAGGATTCTGAGTTGA+TGG + chr8_3:60593657-60593676 None:intergenic 40.0%
TGCTAGAACACAAGAAGTTG+AGG - chr8_3:60593695-60593714 Msa1299420:intron 40.0%
AAATGTCTCCAAGCCATTGA+AGG + chr8_3:60593801-60593820 None:intergenic 40.0%
ACTGCTAAGGGACATCAAAT+GGG - chr8_3:60594324-60594343 Msa1299420:intron 40.0%
! GCAAATTTTCCAGCTTCAAG+AGG - chr8_3:60592531-60592550 Msa1299420:intron 40.0%
AATCAAGAGGCTAATGAGCA+AGG - chr8_3:60593216-60593235 Msa1299420:intron 40.0%
ACTAAGTGTGCATAGAGACA+TGG + chr8_3:60593422-60593441 None:intergenic 40.0%
! GGAAAAGGATTCTGAGTTGA+TGG + chr8_3:60593657-60593676 None:intergenic 40.0%
TGCTAGAACACAAGAAGTTG+AGG - chr8_3:60593695-60593714 Msa1299420:intron 40.0%
AAATGTCTCCAAGCCATTGA+AGG + chr8_3:60593801-60593820 None:intergenic 40.0%
!! GAGTTTTGGCAGTAGTTCTG+AGG - chr8_3:60592897-60592916 Msa1299420:CDS 45.0%
ATAAACGACTCAGCAGCCAT+TGG + chr8_3:60593378-60593397 None:intergenic 45.0%
GAGGCTGCTGAATTAGTACA+TGG - chr8_3:60593714-60593733 Msa1299420:intron 45.0%
GCTGAATTAGTACATGGCCA+AGG - chr8_3:60593720-60593739 Msa1299420:intron 45.0%
AGCACTAACTCAGCATGTTC+AGG + chr8_3:60593747-60593766 None:intergenic 45.0%
CCATTGAAGGATGTCTAGCA+TGG + chr8_3:60593788-60593807 None:intergenic 45.0%
CCATGCTAGACATCCTTCAA+TGG - chr8_3:60593785-60593804 Msa1299420:intron 45.0%
CTAGACATCCTTCAATGGCT+TGG - chr8_3:60593790-60593809 Msa1299420:intron 45.0%
GACTGCTAAGGGACATCAAA+TGG - chr8_3:60594323-60594342 Msa1299420:intron 45.0%
!! GAGTTTTGGCAGTAGTTCTG+AGG - chr8_3:60592897-60592916 Msa1299420:CDS 45.0%
ATAAACGACTCAGCAGCCAT+TGG + chr8_3:60593378-60593397 None:intergenic 45.0%
GAGGCTGCTGAATTAGTACA+TGG - chr8_3:60593714-60593733 Msa1299420:intron 45.0%
GCTGAATTAGTACATGGCCA+AGG - chr8_3:60593720-60593739 Msa1299420:intron 45.0%
AGCACTAACTCAGCATGTTC+AGG + chr8_3:60593747-60593766 None:intergenic 45.0%
CCATTGAAGGATGTCTAGCA+TGG + chr8_3:60593788-60593807 None:intergenic 45.0%
CCATGCTAGACATCCTTCAA+TGG - chr8_3:60593785-60593804 Msa1299420:intron 45.0%
CTAGACATCCTTCAATGGCT+TGG - chr8_3:60593790-60593809 Msa1299420:intron 45.0%
GACTGCTAAGGGACATCAAA+TGG - chr8_3:60594323-60594342 Msa1299420:intron 45.0%
!! TCATTATTTAAAAATTTATT+AGG + chr8_3:60593582-60593601 None:intergenic 5.0%
!!! ATTTTTAAACTTATTTTTAT+TGG - chr8_3:60594269-60594288 Msa1299420:intron 5.0%
!! TCATTATTTAAAAATTTATT+AGG + chr8_3:60593582-60593601 None:intergenic 5.0%
!!! ATTTTTAAACTTATTTTTAT+TGG - chr8_3:60594269-60594288 Msa1299420:intron 5.0%
ACTCAGCATGTTCAGGTCCT+TGG + chr8_3:60593740-60593759 None:intergenic 50.0%
ACTCAGCATGTTCAGGTCCT+TGG + chr8_3:60593740-60593759 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60592223 60594346 60592223 ID=Msa1299420;Name=Msa1299420
chr8_3 mRNA 60592223 60594346 60592223 ID=Msa1299420-mRNA-1;Parent=Msa1299420;Name=Msa1299420-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0.25|0.25|0|0.5|4|164|88
chr8_3 exon 60592223 60592443 60592223 ID=Msa1299420-mRNA-1:exon:17747;Parent=Msa1299420-mRNA-1
chr8_3 exon 60592808 60592965 60592808 ID=Msa1299420-mRNA-1:exon:17746;Parent=Msa1299420-mRNA-1
chr8_3 exon 60594017 60594062 60594017 ID=Msa1299420-mRNA-1:exon:17745;Parent=Msa1299420-mRNA-1
chr8_3 exon 60594341 60594346 60594341 ID=Msa1299420-mRNA-1:exon:17744;Parent=Msa1299420-mRNA-1
chr8_3 CDS 60594341 60594346 60594341 ID=Msa1299420-mRNA-1:cds;Parent=Msa1299420-mRNA-1
chr8_3 CDS 60594017 60594062 60594017 ID=Msa1299420-mRNA-1:cds;Parent=Msa1299420-mRNA-1
chr8_3 CDS 60592808 60592965 60592808 ID=Msa1299420-mRNA-1:cds;Parent=Msa1299420-mRNA-1
chr8_3 CDS 60592387 60592443 60592387 ID=Msa1299420-mRNA-1:cds;Parent=Msa1299420-mRNA-1
chr8_3 three_prime_UTR 60592223 60592386 60592223 ID=Msa1299420-mRNA-1:three_prime_utr;Parent=Msa1299420-mRNA-1
Gene Sequence

>Msa1299420

AGTTTTCTTTGGAAACTTCTATCTTTTTCTGCAAATTTTCCAGCTTCAAGAGCTGGTGCAGCAGCATCAACTCAAACTATGAATAATGAAGCTGGTGCAGCACCATCAACTCAGAATCCTTTTCCTCAAATTCCAATTCAAGTTGCTAGAACACAAGAAGTTGAGGCTGCTGAATTAGTACATGGCCAAGGACCTGAACATGCTGAGTTAGTTAACAATATTAATGTGATAATGTATCTTGATCTATTGTGGGATCAAGATATATAA

Protein sequence

>Msa1299420

SFLWKLLSFSANFPASRAGAAASTQTMNNEAGAAPSTQNPFPQIPIQVARTQEVEAAELVHGQGPEHAELVNNINVIMYLDLLWDQDI*