Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0546050 | Msa0744790 | 0.839990 | 1.138321e-57 | -8.615850e-47 |
| Msa0546050 | Msa0744820 | 0.833538 | 5.035405e-56 | -8.615850e-47 |
| Msa0546050 | Msa0879520 | 0.852137 | 5.622484e-61 | -8.615850e-47 |
| Msa0546050 | Msa1124020 | 0.816225 | 6.170004e-52 | -8.615850e-47 |
| Msa0546050 | Msa1299420 | 0.823680 | 1.215635e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATAATAACAGTGAAAC+TGG | 0.302635 | 4_1:-37182737 | None:intergenic |
| TGTGTTCTAGCAACTTGAAT+TGG | 0.324721 | 4_1:-37182213 | None:intergenic |
| AGCACTAACTCAGCATGTTC+AGG | 0.362910 | 4_1:-37182273 | None:intergenic |
| TGAATTGGAATTTGAGGAAA+AGG | 0.370298 | 4_1:-37182198 | None:intergenic |
| GACTGCCAAGGGACATCAAA+TGG | 0.376551 | 4_1:+37182852 | Msa0546050:three_prime_UTR |
| GCAACTTGAATTGGAATTTG+AGG | 0.378140 | 4_1:-37182204 | None:intergenic |
| TGTGGGATCAAGATATATAA+AGG | 0.423750 | 4_1:+37182692 | Msa0546050:CDS |
| ACTCAGCATGTTCAGGTCCT+TGG | 0.469300 | 4_1:-37182266 | None:intergenic |
| AATAACAGTGAAACTGGATC+TGG | 0.488205 | 4_1:-37182731 | None:intergenic |
| ACTGCCAAGGGACATCAAAT+GGG | 0.526159 | 4_1:+37182853 | Msa0546050:three_prime_UTR |
| TAATGTATCTTGATCTATTG+TGG | 0.528080 | 4_1:+37182674 | Msa0546050:CDS |
| AATGTATCTTGATCTATTGT+GGG | 0.535708 | 4_1:+37182675 | Msa0546050:CDS |
| TTTAATCATATGACTGCCAA+GGG | 0.551224 | 4_1:+37182841 | Msa0546050:three_prime_UTR |
| CAAACTATGAATAATGAAGC+TGG | 0.552669 | 4_1:+37182153 | Msa0546050:exon |
| TGCTAGAACACAAGAAGTTG+AGG | 0.553850 | 4_1:+37182224 | Msa0546050:CDS |
| GAGGTTGCTGAATTAGTACA+TGG | 0.563099 | 4_1:+37182243 | Msa0546050:CDS |
| GCTGAATTAGTACATGGCCA+AGG | 0.648688 | 4_1:+37182249 | Msa0546050:CDS |
| GGGATCAAGATATATAAAGG+AGG | 0.651640 | 4_1:+37182695 | Msa0546050:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTGTATTAAGTTTTTTATT+AGG | + | chr4_1:37182766-37182785 | Msa0546050:three_prime_UTR | 15.0% |
| !!! | AAAAACTACCTTTATCTTTT+TGG | - | chr4_1:37182459-37182478 | None:intergenic | 20.0% |
| !!! | TGCTATATTGTTATATTTTG+TGG | + | chr4_1:37182584-37182603 | Msa0546050:intron | 20.0% |
| ! | TGATATGTCCAAAAAGATAA+AGG | + | chr4_1:37182448-37182467 | Msa0546050:intron | 25.0% |
| !!! | ATTTTCTGTATTTTTTGCAC+AGG | + | chr4_1:37182633-37182652 | Msa0546050:intron | 25.0% |
| ! | TAATGTATCTTGATCTATTG+TGG | + | chr4_1:37182674-37182693 | Msa0546050:CDS | 25.0% |
| ! | AATGTATCTTGATCTATTGT+GGG | + | chr4_1:37182675-37182694 | Msa0546050:CDS | 25.0% |
| ! | GAAAATAATAACAGTGAAAC+TGG | - | chr4_1:37182740-37182759 | None:intergenic | 25.0% |
| CAAACTATGAATAATGAAGC+TGG | + | chr4_1:37182153-37182172 | Msa0546050:exon | 30.0% | |
| TGAATTGGAATTTGAGGAAA+AGG | - | chr4_1:37182201-37182220 | None:intergenic | 30.0% | |
| AGTGAAACAGAAGTTACAAA+AGG | - | chr4_1:37182373-37182392 | None:intergenic | 30.0% | |
| ! | TAAGCATGTTGTTAGTTTGA+TGG | - | chr4_1:37182419-37182438 | None:intergenic | 30.0% |
| TGTGGGATCAAGATATATAA+AGG | + | chr4_1:37182692-37182711 | Msa0546050:CDS | 30.0% | |
| ! | TTTTAATCATATGACTGCCA+AGG | + | chr4_1:37182840-37182859 | Msa0546050:three_prime_UTR | 30.0% |
| TTTAATCATATGACTGCCAA+GGG | + | chr4_1:37182841-37182860 | Msa0546050:three_prime_UTR | 30.0% | |
| GCAACTTGAATTGGAATTTG+AGG | - | chr4_1:37182207-37182226 | None:intergenic | 35.0% | |
| TGTGTTCTAGCAACTTGAAT+TGG | - | chr4_1:37182216-37182235 | None:intergenic | 35.0% | |
| GAAACAGAAGTTACAAAAGG+CGG | - | chr4_1:37182370-37182389 | None:intergenic | 35.0% | |
| GGGATCAAGATATATAAAGG+AGG | + | chr4_1:37182695-37182714 | Msa0546050:exon | 35.0% | |
| AATAACAGTGAAACTGGATC+TGG | - | chr4_1:37182734-37182753 | None:intergenic | 35.0% | |
| TGCTAGAACACAAGAAGTTG+AGG | + | chr4_1:37182224-37182243 | Msa0546050:CDS | 40.0% | |
| GAGGTTGCTGAATTAGTACA+TGG | + | chr4_1:37182243-37182262 | Msa0546050:CDS | 40.0% | |
| AAATGTCTCCAAGCCATTGA+AGG | - | chr4_1:37182330-37182349 | None:intergenic | 40.0% | |
| GCTGAATTAGTACATGGCCA+AGG | + | chr4_1:37182249-37182268 | Msa0546050:CDS | 45.0% | |
| AGCACTAACTCAGCATGTTC+AGG | - | chr4_1:37182276-37182295 | None:intergenic | 45.0% | |
| CCATTGAAGGATGTCTAGCA+TGG | - | chr4_1:37182317-37182336 | None:intergenic | 45.0% | |
| CCATGCTAGACATCCTTCAA+TGG | + | chr4_1:37182314-37182333 | Msa0546050:intron | 45.0% | |
| CTAGACATCCTTCAATGGCT+TGG | + | chr4_1:37182319-37182338 | Msa0546050:intron | 45.0% | |
| !!! | ATTTTTAAACTTATTTTTAT+TGG | + | chr4_1:37182798-37182817 | Msa0546050:three_prime_UTR | 5.0% |
| ACTCAGCATGTTCAGGTCCT+TGG | - | chr4_1:37182269-37182288 | None:intergenic | 50.0% | |
| GACTGCCAAGGGACATCAAA+TGG | + | chr4_1:37182852-37182871 | Msa0546050:three_prime_UTR | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 37182133 | 37182875 | 37182133 | ID=Msa0546050;Name=Msa0546050 |
| chr4_1 | mRNA | 37182133 | 37182875 | 37182133 | ID=Msa0546050-mRNA-1;Parent=Msa0546050;Name=Msa0546050-mRNA-1;_AED=0.63;_eAED=0.63;_QI=26|0|0.5|0.5|0|0|2|164|62 |
| chr4_1 | exon | 37182133 | 37182290 | 37182133 | ID=Msa0546050-mRNA-1:exon:9453;Parent=Msa0546050-mRNA-1 |
| chr4_1 | exon | 37182655 | 37182875 | 37182655 | ID=Msa0546050-mRNA-1:exon:9454;Parent=Msa0546050-mRNA-1 |
| chr4_1 | five_prime_UTR | 37182133 | 37182158 | 37182133 | ID=Msa0546050-mRNA-1:five_prime_utr;Parent=Msa0546050-mRNA-1 |
| chr4_1 | CDS | 37182159 | 37182290 | 37182159 | ID=Msa0546050-mRNA-1:cds;Parent=Msa0546050-mRNA-1 |
| chr4_1 | CDS | 37182655 | 37182711 | 37182655 | ID=Msa0546050-mRNA-1:cds;Parent=Msa0546050-mRNA-1 |
| chr4_1 | three_prime_UTR | 37182712 | 37182875 | 37182712 | ID=Msa0546050-mRNA-1:three_prime_utr;Parent=Msa0546050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
