AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0879520


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATAATAACAGTGAAAC+TGG 0.302635 6_1:+22067762 None:intergenic
TGTGTTCTAGCAACTTGAAT+TGG 0.324721 6_1:+22068286 None:intergenic
GACTGCTAAGGGACATCAAA+TGG 0.354379 6_1:-22067647 Msa0879520:three_prime_UTR
AGCACTAACTCAGCATGTTC+AGG 0.362910 6_1:+22068226 None:intergenic
TGAATTGGAATTTGAGGAAA+AGG 0.370298 6_1:+22068301 None:intergenic
GCAACTTGAATTGGAATTTG+AGG 0.378140 6_1:+22068295 None:intergenic
TGTGGGATCAAGATATATAA+AGG 0.423750 6_1:-22067807 Msa0879520:exon
TCAAAATTACCTCTTGAAGC+TGG 0.465001 6_1:+22069430 None:intergenic
ACTCAGCATGTTCAGGTCCT+TGG 0.469300 6_1:+22068233 None:intergenic
GGAAAAGGATTCTGAGTTGA+TGG 0.475687 6_1:+22068316 None:intergenic
TTTAATCATATGACTGCTAA+GGG 0.486787 6_1:-22067658 Msa0879520:three_prime_UTR
AATAACAGTGAAACTGGATC+TGG 0.488205 6_1:+22067768 None:intergenic
ACTGCTAAGGGACATCAAAT+GGG 0.515323 6_1:-22067646 Msa0879520:three_prime_UTR
TAATGTATCTTGATCTATTG+TGG 0.528080 6_1:-22067825 Msa0879520:CDS
AATGTATCTTGATCTATTGT+GGG 0.535708 6_1:-22067824 Msa0879520:CDS
CAAACTATGAATAATGAAGC+TGG 0.552669 6_1:-22068346 Msa0879520:CDS
TGCTAGAACACAAGAAGTTG+AGG 0.602315 6_1:-22068275 Msa0879520:CDS
GAGGCTGCTGAATTAGTACA+TGG 0.624946 6_1:-22068256 Msa0879520:CDS
GCTGAATTAGTACATGGCCA+AGG 0.646206 6_1:-22068250 Msa0879520:CDS
GGGATCAAGATATATAAAGG+AGG 0.651640 6_1:-22067804 Msa0879520:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATCTTTTTCTGATATA+TGG - chr6_1:22067654-22067673 Msa0879520:three_prime_UTR 15.0%
!! AAAATGTAACAAACATAATT+TGG + chr6_1:22068062-22068081 None:intergenic 15.0%
!! AATATATGATTCTTTATCTT+TGG - chr6_1:22068298-22068317 Msa0879520:CDS 15.0%
!! TCTTTACTAATTATTATCAA+AGG + chr6_1:22068556-22068575 None:intergenic 15.0%
!!! AAATTTTTAAATAATGAAGC+TGG - chr6_1:22069008-22069027 Msa0879520:intron 15.0%
!!! ACTGTATTAAGTTTTTTATT+AGG - chr6_1:22069660-22069679 Msa0879520:intron 15.0%
!!! ATTAATCTTTTTCTGATATA+TGG - chr6_1:22067654-22067673 Msa0879520:three_prime_UTR 15.0%
!! AAAATGTAACAAACATAATT+TGG + chr6_1:22068062-22068081 None:intergenic 15.0%
!! AATATATGATTCTTTATCTT+TGG - chr6_1:22068298-22068317 Msa0879520:CDS 15.0%
!! TCTTTACTAATTATTATCAA+AGG + chr6_1:22068556-22068575 None:intergenic 15.0%
!!! AAATTTTTAAATAATGAAGC+TGG - chr6_1:22069008-22069027 Msa0879520:intron 15.0%
!!! ACTGTATTAAGTTTTTTATT+AGG - chr6_1:22069660-22069679 Msa0879520:intron 15.0%
!! ACAAACATAATTTGGATAAA+AGG + chr6_1:22068054-22068073 None:intergenic 20.0%
!!! AGTTCTAACATTTATAGTAA+TGG - chr6_1:22068114-22068133 Msa0879520:intron 20.0%
!! TGGAGAATAGATAAAATATA+AGG - chr6_1:22068416-22068435 Msa0879520:intron 20.0%
!!! ATGAAGGTATCTTTAATAAA+AGG + chr6_1:22068529-22068548 None:intergenic 20.0%
!! TATTATCAAAGGTTAAATGA+AGG + chr6_1:22068545-22068564 None:intergenic 20.0%
!! AATATCAAAAGATAAGTTAG+CGG + chr6_1:22068603-22068622 None:intergenic 20.0%
!! AATTTATTAGGTGAGTATTT+AGG + chr6_1:22068993-22069012 None:intergenic 20.0%
!!! AAAAACTACCTTTATCTTTT+TGG + chr6_1:22069353-22069372 None:intergenic 20.0%
!!! TGCTATATTGTTATATTTTG+TGG - chr6_1:22069477-22069496 Msa0879520:intron 20.0%
!! ACAAACATAATTTGGATAAA+AGG + chr6_1:22068054-22068073 None:intergenic 20.0%
!!! AGTTCTAACATTTATAGTAA+TGG - chr6_1:22068114-22068133 Msa0879520:intron 20.0%
!! TGGAGAATAGATAAAATATA+AGG - chr6_1:22068416-22068435 Msa0879520:intron 20.0%
!!! ATGAAGGTATCTTTAATAAA+AGG + chr6_1:22068529-22068548 None:intergenic 20.0%
!! TATTATCAAAGGTTAAATGA+AGG + chr6_1:22068545-22068564 None:intergenic 20.0%
!! AATATCAAAAGATAAGTTAG+CGG + chr6_1:22068603-22068622 None:intergenic 20.0%
!! AATTTATTAGGTGAGTATTT+AGG + chr6_1:22068993-22069012 None:intergenic 20.0%
!!! AAAAACTACCTTTATCTTTT+TGG + chr6_1:22069353-22069372 None:intergenic 20.0%
!!! TGCTATATTGTTATATTTTG+TGG - chr6_1:22069477-22069496 Msa0879520:intron 20.0%
!! TTTTCTCTTTTTATCAGCTT+TGG - chr6_1:22067913-22067932 Msa0879520:intron 25.0%
!! ATTTTATCTATTCTCCAAAG+TGG + chr6_1:22068413-22068432 None:intergenic 25.0%
!! GTTTGTTACATTAATTGCTA+TGG - chr6_1:22068476-22068495 Msa0879520:intron 25.0%
! TACAATATACACAAATGACA+AGG + chr6_1:22068690-22068709 None:intergenic 25.0%
!! TGTCATTTGTGTATATTGTA+TGG - chr6_1:22068690-22068709 Msa0879520:intron 25.0%
! AGGACTGTAATAATGTTTAA+GGG + chr6_1:22068973-22068992 None:intergenic 25.0%
! TAGGACTGTAATAATGTTTA+AGG + chr6_1:22068974-22068993 None:intergenic 25.0%
! TGATATGTCCAAAAAGATAA+AGG - chr6_1:22069342-22069361 Msa0879520:intron 25.0%
!!! ATTTTCTGTATTTTTTGCAC+AGG - chr6_1:22069527-22069546 Msa0879520:intron 25.0%
! TAATGTATCTTGATCTATTG+TGG - chr6_1:22069568-22069587 Msa0879520:intron 25.0%
! AATGTATCTTGATCTATTGT+GGG - chr6_1:22069569-22069588 Msa0879520:intron 25.0%
! GAAAATAATAACAGTGAAAC+TGG + chr6_1:22069634-22069653 None:intergenic 25.0%
!! TTTTAATCATATGACTGCTA+AGG - chr6_1:22069734-22069753 Msa0879520:intron 25.0%
! TTTAATCATATGACTGCTAA+GGG - chr6_1:22069735-22069754 Msa0879520:intron 25.0%
!! TTTTCTCTTTTTATCAGCTT+TGG - chr6_1:22067913-22067932 Msa0879520:intron 25.0%
!! ATTTTATCTATTCTCCAAAG+TGG + chr6_1:22068413-22068432 None:intergenic 25.0%
!! GTTTGTTACATTAATTGCTA+TGG - chr6_1:22068476-22068495 Msa0879520:intron 25.0%
! TACAATATACACAAATGACA+AGG + chr6_1:22068690-22068709 None:intergenic 25.0%
!! TGTCATTTGTGTATATTGTA+TGG - chr6_1:22068690-22068709 Msa0879520:intron 25.0%
! AGGACTGTAATAATGTTTAA+GGG + chr6_1:22068973-22068992 None:intergenic 25.0%
! TAGGACTGTAATAATGTTTA+AGG + chr6_1:22068974-22068993 None:intergenic 25.0%
! TGATATGTCCAAAAAGATAA+AGG - chr6_1:22069342-22069361 Msa0879520:intron 25.0%
!!! ATTTTCTGTATTTTTTGCAC+AGG - chr6_1:22069527-22069546 Msa0879520:intron 25.0%
! TAATGTATCTTGATCTATTG+TGG - chr6_1:22069568-22069587 Msa0879520:intron 25.0%
! AATGTATCTTGATCTATTGT+GGG - chr6_1:22069569-22069588 Msa0879520:intron 25.0%
! GAAAATAATAACAGTGAAAC+TGG + chr6_1:22069634-22069653 None:intergenic 25.0%
!! TTTTAATCATATGACTGCTA+AGG - chr6_1:22069734-22069753 Msa0879520:intron 25.0%
! TTTAATCATATGACTGCTAA+GGG - chr6_1:22069735-22069754 Msa0879520:intron 25.0%
CATAATTTGGATAAAAGGAC+TGG + chr6_1:22068049-22068068 None:intergenic 30.0%
ATATATATGTGTGTCTGTCT+AGG - chr6_1:22068162-22068181 Msa0879520:intron 30.0%
GGAACTAAGGAAGAAAAATT+AGG + chr6_1:22068217-22068236 None:intergenic 30.0%
!! ATTTGAGTTCTAAGGAACTA+AGG + chr6_1:22068230-22068249 None:intergenic 30.0%
! TAGAGATCATTTGAGTTCTA+AGG + chr6_1:22068238-22068257 None:intergenic 30.0%
! CTTTAATAAAAGGTTGACTC+AGG + chr6_1:22068519-22068538 None:intergenic 30.0%
TCTAGAAAAGATGAATCAAG+AGG - chr6_1:22068626-22068645 Msa0879520:intron 30.0%
! TGCATGATTTTTAATGCCTA+TGG - chr6_1:22068782-22068801 Msa0879520:intron 30.0%
ATAAAAAACTCAGCAGACAT+AGG + chr6_1:22068892-22068911 None:intergenic 30.0%
CAAACTATGAATAATGAAGC+TGG - chr6_1:22069047-22069066 Msa0879520:intron 30.0%
TGAATTGGAATTTGAGGAAA+AGG + chr6_1:22069095-22069114 None:intergenic 30.0%
TGTGGGATCAAGATATATAA+AGG - chr6_1:22069586-22069605 Msa0879520:intron 30.0%
CATAATTTGGATAAAAGGAC+TGG + chr6_1:22068049-22068068 None:intergenic 30.0%
ATATATATGTGTGTCTGTCT+AGG - chr6_1:22068162-22068181 Msa0879520:intron 30.0%
GGAACTAAGGAAGAAAAATT+AGG + chr6_1:22068217-22068236 None:intergenic 30.0%
!! ATTTGAGTTCTAAGGAACTA+AGG + chr6_1:22068230-22068249 None:intergenic 30.0%
! TAGAGATCATTTGAGTTCTA+AGG + chr6_1:22068238-22068257 None:intergenic 30.0%
! CTTTAATAAAAGGTTGACTC+AGG + chr6_1:22068519-22068538 None:intergenic 30.0%
TCTAGAAAAGATGAATCAAG+AGG - chr6_1:22068626-22068645 Msa0879520:intron 30.0%
! TGCATGATTTTTAATGCCTA+TGG - chr6_1:22068782-22068801 Msa0879520:intron 30.0%
ATAAAAAACTCAGCAGACAT+AGG + chr6_1:22068892-22068911 None:intergenic 30.0%
CAAACTATGAATAATGAAGC+TGG - chr6_1:22069047-22069066 Msa0879520:intron 30.0%
TGAATTGGAATTTGAGGAAA+AGG + chr6_1:22069095-22069114 None:intergenic 30.0%
TGTGGGATCAAGATATATAA+AGG - chr6_1:22069586-22069605 Msa0879520:intron 30.0%
! AAGCTTCAAGTTAGAATGTG+TGG - chr6_1:22067843-22067862 Msa0879520:CDS 35.0%
! AGCTTCAAGTTAGAATGTGT+GGG - chr6_1:22067844-22067863 Msa0879520:CDS 35.0%
TCAAAATTACCTCTTGAAGC+TGG + chr6_1:22067966-22067985 None:intergenic 35.0%
!! TTCTGAGGTTCTAGTTATAG+AGG - chr6_1:22068335-22068354 Msa0879520:CDS 35.0%
! TCTGAGGTTCTAGTTATAGA+GGG - chr6_1:22068336-22068355 Msa0879520:CDS 35.0%
TGCATAGAAATTGACCACTT+TGG - chr6_1:22068396-22068415 Msa0879520:intron 35.0%
GCAACTTGAATTGGAATTTG+AGG + chr6_1:22069101-22069120 None:intergenic 35.0%
TGTGTTCTAGCAACTTGAAT+TGG + chr6_1:22069110-22069129 None:intergenic 35.0%
AGTGAAACAGAAGTTGCAAA+AGG + chr6_1:22069267-22069286 None:intergenic 35.0%
TAAGCATGTTGTTAGTTCGA+TGG + chr6_1:22069313-22069332 None:intergenic 35.0%
GGGATCAAGATATATAAAGG+AGG - chr6_1:22069589-22069608 Msa0879520:intron 35.0%
AATAACAGTGAAACTGGATC+TGG + chr6_1:22069628-22069647 None:intergenic 35.0%
! AAGCTTCAAGTTAGAATGTG+TGG - chr6_1:22067843-22067862 Msa0879520:CDS 35.0%
! AGCTTCAAGTTAGAATGTGT+GGG - chr6_1:22067844-22067863 Msa0879520:CDS 35.0%
TCAAAATTACCTCTTGAAGC+TGG + chr6_1:22067966-22067985 None:intergenic 35.0%
!! TTCTGAGGTTCTAGTTATAG+AGG - chr6_1:22068335-22068354 Msa0879520:CDS 35.0%
! TCTGAGGTTCTAGTTATAGA+GGG - chr6_1:22068336-22068355 Msa0879520:CDS 35.0%
TGCATAGAAATTGACCACTT+TGG - chr6_1:22068396-22068415 Msa0879520:intron 35.0%
GCAACTTGAATTGGAATTTG+AGG + chr6_1:22069101-22069120 None:intergenic 35.0%
TGTGTTCTAGCAACTTGAAT+TGG + chr6_1:22069110-22069129 None:intergenic 35.0%
AGTGAAACAGAAGTTGCAAA+AGG + chr6_1:22069267-22069286 None:intergenic 35.0%
TAAGCATGTTGTTAGTTCGA+TGG + chr6_1:22069313-22069332 None:intergenic 35.0%
GGGATCAAGATATATAAAGG+AGG - chr6_1:22069589-22069608 Msa0879520:intron 35.0%
AATAACAGTGAAACTGGATC+TGG + chr6_1:22069628-22069647 None:intergenic 35.0%
! GCAAATTTTCCAGCTTCAAG+AGG - chr6_1:22067954-22067973 Msa0879520:intron 40.0%
AACTAGAACCTCAGAACTAC+TGG + chr6_1:22068331-22068350 None:intergenic 40.0%
AATCAAGAGGCTAATGAGCA+AGG - chr6_1:22068639-22068658 Msa0879520:intron 40.0%
ACTAAGTGTGCATAGAGACA+TGG + chr6_1:22068845-22068864 None:intergenic 40.0%
! GGAAAAGGATTCTGAGTTGA+TGG + chr6_1:22069080-22069099 None:intergenic 40.0%
TGCTAGAACACAAGAAGTTG+AGG - chr6_1:22069118-22069137 Msa0879520:intron 40.0%
AAATGTCTCCAAGCCATTGA+AGG + chr6_1:22069224-22069243 None:intergenic 40.0%
ACTGCTAAGGGACATCAAAT+GGG - chr6_1:22069747-22069766 Msa0879520:intron 40.0%
! GCAAATTTTCCAGCTTCAAG+AGG - chr6_1:22067954-22067973 Msa0879520:intron 40.0%
AACTAGAACCTCAGAACTAC+TGG + chr6_1:22068331-22068350 None:intergenic 40.0%
AATCAAGAGGCTAATGAGCA+AGG - chr6_1:22068639-22068658 Msa0879520:intron 40.0%
ACTAAGTGTGCATAGAGACA+TGG + chr6_1:22068845-22068864 None:intergenic 40.0%
! GGAAAAGGATTCTGAGTTGA+TGG + chr6_1:22069080-22069099 None:intergenic 40.0%
TGCTAGAACACAAGAAGTTG+AGG - chr6_1:22069118-22069137 Msa0879520:intron 40.0%
AAATGTCTCCAAGCCATTGA+AGG + chr6_1:22069224-22069243 None:intergenic 40.0%
!! GAGTTTTGCCAGTAGTTCTG+AGG - chr6_1:22068320-22068339 Msa0879520:CDS 45.0%
ATAAACGACTCAGCAGCCAT+AGG + chr6_1:22068801-22068820 None:intergenic 45.0%
GAGGCTGCTGAATTAGTACA+TGG - chr6_1:22069137-22069156 Msa0879520:intron 45.0%
GCTGAATTAGTACATGGCCA+AGG - chr6_1:22069143-22069162 Msa0879520:intron 45.0%
AGCACTAACTCAGCATGTTC+AGG + chr6_1:22069170-22069189 None:intergenic 45.0%
CCATTGAAGGATGTCTAGCA+TGG + chr6_1:22069211-22069230 None:intergenic 45.0%
CCATGCTAGACATCCTTCAA+TGG - chr6_1:22069208-22069227 Msa0879520:intron 45.0%
CTAGACATCCTTCAATGGCT+TGG - chr6_1:22069213-22069232 Msa0879520:intron 45.0%
GACTGCTAAGGGACATCAAA+TGG - chr6_1:22069746-22069765 Msa0879520:intron 45.0%
!! GAGTTTTGCCAGTAGTTCTG+AGG - chr6_1:22068320-22068339 Msa0879520:CDS 45.0%
ATAAACGACTCAGCAGCCAT+AGG + chr6_1:22068801-22068820 None:intergenic 45.0%
GAGGCTGCTGAATTAGTACA+TGG - chr6_1:22069137-22069156 Msa0879520:intron 45.0%
GCTGAATTAGTACATGGCCA+AGG - chr6_1:22069143-22069162 Msa0879520:intron 45.0%
AGCACTAACTCAGCATGTTC+AGG + chr6_1:22069170-22069189 None:intergenic 45.0%
CCATTGAAGGATGTCTAGCA+TGG + chr6_1:22069211-22069230 None:intergenic 45.0%
CCATGCTAGACATCCTTCAA+TGG - chr6_1:22069208-22069227 Msa0879520:intron 45.0%
CTAGACATCCTTCAATGGCT+TGG - chr6_1:22069213-22069232 Msa0879520:intron 45.0%
GACTGCTAAGGGACATCAAA+TGG - chr6_1:22069746-22069765 Msa0879520:intron 45.0%
!! TCATTATTTAAAAATTTATT+AGG + chr6_1:22069005-22069024 None:intergenic 5.0%
!!! ATTTTTAAACTTATTTTTAT+TGG - chr6_1:22069692-22069711 Msa0879520:intron 5.0%
!! TCATTATTTAAAAATTTATT+AGG + chr6_1:22069005-22069024 None:intergenic 5.0%
!!! ATTTTTAAACTTATTTTTAT+TGG - chr6_1:22069692-22069711 Msa0879520:intron 5.0%
ACTCAGCATGTTCAGGTCCT+TGG + chr6_1:22069163-22069182 None:intergenic 50.0%
ACTCAGCATGTTCAGGTCCT+TGG + chr6_1:22069163-22069182 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 22067646 22069769 22067646 ID=Msa0879520;Name=Msa0879520
chr6_1 mRNA 22067646 22069769 22067646 ID=Msa0879520-mRNA-1;Parent=Msa0879520;Name=Msa0879520-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0.25|0.25|0|0.25|4|164|88
chr6_1 exon 22067646 22067866 22067646 ID=Msa0879520-mRNA-1:exon:5999;Parent=Msa0879520-mRNA-1
chr6_1 exon 22068231 22068388 22068231 ID=Msa0879520-mRNA-1:exon:5998;Parent=Msa0879520-mRNA-1
chr6_1 exon 22069440 22069485 22069440 ID=Msa0879520-mRNA-1:exon:5997;Parent=Msa0879520-mRNA-1
chr6_1 exon 22069764 22069769 22069764 ID=Msa0879520-mRNA-1:exon:5996;Parent=Msa0879520-mRNA-1
chr6_1 CDS 22069764 22069769 22069764 ID=Msa0879520-mRNA-1:cds;Parent=Msa0879520-mRNA-1
chr6_1 CDS 22069440 22069485 22069440 ID=Msa0879520-mRNA-1:cds;Parent=Msa0879520-mRNA-1
chr6_1 CDS 22068231 22068388 22068231 ID=Msa0879520-mRNA-1:cds;Parent=Msa0879520-mRNA-1
chr6_1 CDS 22067810 22067866 22067810 ID=Msa0879520-mRNA-1:cds;Parent=Msa0879520-mRNA-1
chr6_1 three_prime_UTR 22067646 22067809 22067646 ID=Msa0879520-mRNA-1:three_prime_utr;Parent=Msa0879520-mRNA-1
Gene Sequence

>Msa0879520

AGTTTTCTTTGGAAACTTCTATCTTTTTCTGCAAATTTTCCAGCTTCAAGAGCTGGTGCAGCAGCATCAACTCAAACTATGAATAATGAAGCTGGTGCAGCACCATCAACTCAGAATCCTTTTCCTCAAATTCCAATTCAAGTTGCTAGAACACAAGAAGTTGAGGCTGCTGAATTAGTACATGGCCAAGGACCTGAACATGCTGAGTTAGTTAACAATATTAATGTGATAATGTATCTTGATCTATTGTGGGATCAAGATATATAA

Protein sequence

>Msa0879520

SFLWKLLSFSANFPASRAGAAASTQTMNNEAGAAPSTQNPFPQIPIQVARTQEVEAAELVHGQGPEHAELVNNINVIMYLDLLWDQDI*