Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0744790 | Msa0744820 | 0.943559 | 9.664123e-103 | -8.615850e-47 |
| Msa0744820 | Msa0879520 | 0.965780 | 4.741419e-125 | -8.615850e-47 |
| Msa0744820 | Msa1299420 | 0.955558 | 2.297975e-113 | -8.615850e-47 |
| Msa0546050 | Msa0744820 | 0.833538 | 5.035405e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATAATAACAGTGAAAC+TGG | 0.302635 | 5_1:+65856521 | None:intergenic |
| TGTGTTCTAGCAACTTGAAT+TGG | 0.324721 | 5_1:+65857045 | None:intergenic |
| GACTGCTAAGGGACATCAAA+TGG | 0.354379 | 5_1:-65856406 | Msa0744820:three_prime_UTR |
| AGCACTAACTCAGCATGTTC+AGG | 0.362910 | 5_1:+65856985 | None:intergenic |
| TGAATTGGAATTTGAGGAAA+AGG | 0.370298 | 5_1:+65857060 | None:intergenic |
| GCAACTTGAATTGGAATTTG+AGG | 0.378140 | 5_1:+65857054 | None:intergenic |
| TGTGGGATCAAGATATATAA+AGG | 0.423750 | 5_1:-65856566 | Msa0744820:exon |
| ACTCAGCATGTTCAGGTCCT+TGG | 0.469300 | 5_1:+65856992 | None:intergenic |
| GGAAAAGGATTCTGAGTTGA+TGG | 0.475687 | 5_1:+65857075 | None:intergenic |
| TTTAATCATATGACTGCTAA+GGG | 0.486787 | 5_1:-65856417 | Msa0744820:three_prime_UTR |
| AATAACAGTGAAACTGGATC+TGG | 0.488205 | 5_1:+65856527 | None:intergenic |
| ACTGCTAAGGGACATCAAAT+GGG | 0.515323 | 5_1:-65856405 | Msa0744820:three_prime_UTR |
| TAATGTATCTTGATCTATTG+TGG | 0.528080 | 5_1:-65856584 | Msa0744820:CDS |
| AATGTATCTTGATCTATTGT+GGG | 0.535708 | 5_1:-65856583 | Msa0744820:CDS |
| CAAACTATGAATAATGAAGC+TGG | 0.552669 | 5_1:-65857105 | Msa0744820:exon |
| TGCTAGAACACAAGAAGTTG+AGG | 0.602315 | 5_1:-65857034 | Msa0744820:CDS |
| GAGGCTGCTGAATTAGTACA+TGG | 0.624946 | 5_1:-65857015 | Msa0744820:CDS |
| GCTGAATTAGTACATGGCCA+AGG | 0.646206 | 5_1:-65857009 | Msa0744820:CDS |
| GGGATCAAGATATATAAAGG+AGG | 0.651640 | 5_1:-65856563 | Msa0744820:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTGTATTAAGTTTTTTATT+AGG | - | chr5_1:65857038-65857057 | Msa0744820:CDS | 15.0% |
| !!! | AAAAACTACCTTTATCTTTT+TGG | + | chr5_1:65856731-65856750 | None:intergenic | 20.0% |
| !!! | TGCTATATTGTTATATTTTG+TGG | - | chr5_1:65856855-65856874 | Msa0744820:intron | 20.0% |
| ! | TGATATGTCCAAAAAGATAA+AGG | - | chr5_1:65856720-65856739 | Msa0744820:intron | 25.0% |
| !!! | ATTTTCTGTATTTTTTGCAC+AGG | - | chr5_1:65856905-65856924 | Msa0744820:intron | 25.0% |
| ! | TAATGTATCTTGATCTATTG+TGG | - | chr5_1:65856946-65856965 | Msa0744820:intron | 25.0% |
| ! | AATGTATCTTGATCTATTGT+GGG | - | chr5_1:65856947-65856966 | Msa0744820:intron | 25.0% |
| ! | GAAAATAATAACAGTGAAAC+TGG | + | chr5_1:65857012-65857031 | None:intergenic | 25.0% |
| !! | TTTTAATCATATGACTGCTA+AGG | - | chr5_1:65857112-65857131 | Msa0744820:exon | 25.0% |
| ! | TTTAATCATATGACTGCTAA+GGG | - | chr5_1:65857113-65857132 | Msa0744820:exon | 25.0% |
| CAAACTATGAATAATGAAGC+TGG | - | chr5_1:65856425-65856444 | Msa0744820:three_prime_UTR | 30.0% | |
| TGAATTGGAATTTGAGGAAA+AGG | + | chr5_1:65856473-65856492 | None:intergenic | 30.0% | |
| AGTGAAACAGAAGTTACAAA+AGG | + | chr5_1:65856645-65856664 | None:intergenic | 30.0% | |
| TGTGGGATCAAGATATATAA+AGG | - | chr5_1:65856964-65856983 | Msa0744820:intron | 30.0% | |
| GCAACTTGAATTGGAATTTG+AGG | + | chr5_1:65856479-65856498 | None:intergenic | 35.0% | |
| TGTGTTCTAGCAACTTGAAT+TGG | + | chr5_1:65856488-65856507 | None:intergenic | 35.0% | |
| TAAGCATGTTGTTAGTTCGA+TGG | + | chr5_1:65856691-65856710 | None:intergenic | 35.0% | |
| GGGATCAAGATATATAAAGG+AGG | - | chr5_1:65856967-65856986 | Msa0744820:intron | 35.0% | |
| AATAACAGTGAAACTGGATC+TGG | + | chr5_1:65857006-65857025 | None:intergenic | 35.0% | |
| ! | GGAAAAGGATTCTGAGTTGA+TGG | + | chr5_1:65856458-65856477 | None:intergenic | 40.0% |
| TGCTAGAACACAAGAAGTTG+AGG | - | chr5_1:65856496-65856515 | Msa0744820:three_prime_UTR | 40.0% | |
| AAATGTCTCCAAGCCATTGA+AGG | + | chr5_1:65856602-65856621 | None:intergenic | 40.0% | |
| GAGGCTGCTGAATTAGTACA+TGG | - | chr5_1:65856515-65856534 | Msa0744820:three_prime_UTR | 45.0% | |
| GCTGAATTAGTACATGGCCA+AGG | - | chr5_1:65856521-65856540 | Msa0744820:three_prime_UTR | 45.0% | |
| AGCACTAACTCAGCATGTTC+AGG | + | chr5_1:65856548-65856567 | None:intergenic | 45.0% | |
| CCATTGAAGGATGTCTAGCA+TGG | + | chr5_1:65856589-65856608 | None:intergenic | 45.0% | |
| CCATGCTAGACATCCTTCAA+TGG | - | chr5_1:65856586-65856605 | Msa0744820:CDS | 45.0% | |
| CTAGACATCCTTCAATGGCT+TGG | - | chr5_1:65856591-65856610 | Msa0744820:CDS | 45.0% | |
| GACTGCTAAGGGACATCAAA+TGG | - | chr5_1:65857124-65857143 | Msa0744820:five_prime_UTR | 45.0% | |
| !!! | ATTTTTAAACTTATTTTTAT+TGG | - | chr5_1:65857070-65857089 | Msa0744820:CDS | 5.0% |
| ACTCAGCATGTTCAGGTCCT+TGG | + | chr5_1:65856541-65856560 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 65856405 | 65857147 | 65856405 | ID=Msa0744820;Name=Msa0744820 |
| chr5_1 | mRNA | 65856405 | 65857147 | 65856405 | ID=Msa0744820-mRNA-1;Parent=Msa0744820;Name=Msa0744820-mRNA-1;_AED=0.24;_eAED=0.24;_QI=26|0|0.5|0.5|0|0.5|2|164|62 |
| chr5_1 | exon | 65856405 | 65856625 | 65856405 | ID=Msa0744820-mRNA-1:exon:19579;Parent=Msa0744820-mRNA-1 |
| chr5_1 | exon | 65856990 | 65857147 | 65856990 | ID=Msa0744820-mRNA-1:exon:19578;Parent=Msa0744820-mRNA-1 |
| chr5_1 | five_prime_UTR | 65857122 | 65857147 | 65857122 | ID=Msa0744820-mRNA-1:five_prime_utr;Parent=Msa0744820-mRNA-1 |
| chr5_1 | CDS | 65856990 | 65857121 | 65856990 | ID=Msa0744820-mRNA-1:cds;Parent=Msa0744820-mRNA-1 |
| chr5_1 | CDS | 65856569 | 65856625 | 65856569 | ID=Msa0744820-mRNA-1:cds;Parent=Msa0744820-mRNA-1 |
| chr5_1 | three_prime_UTR | 65856405 | 65856568 | 65856405 | ID=Msa0744820-mRNA-1:three_prime_utr;Parent=Msa0744820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
