Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0747330 | MCI29318.1 | 98.837 | 86 | 1 | 0 | 1 | 86 | 1 | 86 | 1.78e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0747330 | sp|Q9ZW34|ENO3_ARATH | 87.209 | 86 | 11 | 0 | 1 | 86 | 1 | 86 | 2.54e-47 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0747330 | A0A392QYE0 | 98.837 | 86 | 1 | 0 | 1 | 86 | 1 | 86 | 8.51e-56 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0054530 | Msa0747330 | 0.805490 | 1.301704e-49 | -8.615850e-47 |
| Msa0747330 | Msa0825530 | 0.817494 | 3.203778e-52 | -8.615850e-47 |
| Msa0747330 | Msa0864760 | 0.849484 | 3.139532e-60 | -8.615850e-47 |
| Msa0747330 | Msa1384650 | 0.821599 | 3.705619e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0747330 | MtrunA17_Chr5g0447301 | 100.000 | 87 | 0 | 0 | 1 | 87 | 1 | 87 | 3.82e-58 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0747330 | AT2G29560.1 | 87.209 | 86 | 11 | 0 | 1 | 86 | 1 | 86 | 2.58e-48 | 159 |
Find 25 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACGGGGTCGGAGGTTT+TGG | 0.248202 | 5_1:+71041687 | None:intergenic |
| GGCTCTGACGGCGGCATTGA+CGG | 0.326530 | 5_1:+71041708 | None:intergenic |
| TGACGGCGTCTTCGAGTTTA+CGG | 0.335239 | 5_1:+71041725 | None:intergenic |
| CATTTCTACGATCGATTCTT+CGG | 0.368421 | 5_1:+71041783 | None:intergenic |
| TTTCGTGCTTCTGTTCCATC+TGG | 0.400465 | 5_1:-71041455 | Msa0747330:CDS |
| GATATTACGATGAAGAGAAC+GGG | 0.448791 | 5_1:+71041673 | None:intergenic |
| GACGGCGTCTTCGAGTTTAC+GGG | 0.479590 | 5_1:+71041726 | None:intergenic |
| TACAAATCAACTTCAACAGT+TGG | 0.502701 | 5_1:+71041494 | None:intergenic |
| GTTCCATCTGGAAACTCCAC+TGG | 0.529470 | 5_1:-71041443 | Msa0747330:CDS |
| CGTGAAAGCCTTTCTCAGAA+TGG | 0.532502 | 5_1:+71041935 | None:intergenic |
| AGGTAGGACCATTCTGAGAA+AGG | 0.539368 | 5_1:-71041943 | Msa0747330:five_prime_UTR |
| AGATATTACGATGAAGAGAA+CGG | 0.576440 | 5_1:+71041672 | None:intergenic |
| GATGAAGAGAACGGGGTCGG+AGG | 0.585100 | 5_1:+71041681 | None:intergenic |
| ATGCCAGTGGAGTTTCCAGA+TGG | 0.588263 | 5_1:+71041440 | None:intergenic |
| TTTATCAAGATATTCTTGAA+CGG | 0.588908 | 5_1:+71041759 | None:intergenic |
| TACGATGAAGAGAACGGGGT+CGG | 0.599870 | 5_1:+71041678 | None:intergenic |
| GAATACGACGACGTTTAACA+AGG | 0.623272 | 5_1:-71041963 | None:intergenic |
| AGGCAAATTCTTGATAGTAG+AGG | 0.625871 | 5_1:-71041521 | Msa0747330:CDS |
| CACGCGCTCGGAGATGAGAG+TGG | 0.636109 | 5_1:+71041821 | None:intergenic |
| ATTAACATCTTACATGCCAG+TGG | 0.640506 | 5_1:+71041427 | None:intergenic |
| TGATGAAATGAATGAGGTCA+AGG | 0.644784 | 5_1:+71041844 | None:intergenic |
| GAGTGGTGATGAAATGAATG+AGG | 0.671810 | 5_1:+71041838 | None:intergenic |
| ACGACGACGTTTAACAAGGT+AGG | 0.672274 | 5_1:-71041959 | Msa0747330:five_prime_UTR |
| TGATTACGAAAGTAAAAGCG+AGG | 0.674184 | 5_1:-71041541 | Msa0747330:CDS |
| ATATTACGATGAAGAGAACG+GGG | 0.743082 | 5_1:+71041674 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATCAAGATATTCTTGAA+CGG | + | chr5_1:71041638-71041657 | None:intergenic | 20.0% |
| !! | TATGAAAAAAGGAGTACAAT+CGG | - | chr5_1:71041830-71041849 | Msa0747330:five_prime_UTR | 25.0% |
| AGATATTACGATGAAGAGAA+CGG | + | chr5_1:71041725-71041744 | None:intergenic | 30.0% | |
| CAGTCGAATCATATGAAAAA+AGG | - | chr5_1:71041819-71041838 | Msa0747330:five_prime_UTR | 30.0% | |
| TACAAATCAACTTCAACAGT+TGG | + | chr5_1:71041903-71041922 | None:intergenic | 30.0% | |
| TGATGAAATGAATGAGGTCA+AGG | + | chr5_1:71041553-71041572 | None:intergenic | 35.0% | |
| ! | CATTTCTACGATCGATTCTT+CGG | + | chr5_1:71041614-71041633 | None:intergenic | 35.0% |
| ATATTACGATGAAGAGAACG+GGG | + | chr5_1:71041723-71041742 | None:intergenic | 35.0% | |
| GATATTACGATGAAGAGAAC+GGG | + | chr5_1:71041724-71041743 | None:intergenic | 35.0% | |
| TGATTACGAAAGTAAAAGCG+AGG | - | chr5_1:71041853-71041872 | Msa0747330:five_prime_UTR | 35.0% | |
| ! | AGGCAAATTCTTGATAGTAG+AGG | - | chr5_1:71041873-71041892 | Msa0747330:five_prime_UTR | 35.0% |
| ! | GAGTGGTGATGAAATGAATG+AGG | + | chr5_1:71041559-71041578 | None:intergenic | 40.0% |
| ! | AGGTAGGACCATTCTGAGAA+AGG | - | chr5_1:71041451-71041470 | Msa0747330:CDS | 45.0% |
| CGTGAAAGCCTTTCTCAGAA+TGG | + | chr5_1:71041462-71041481 | None:intergenic | 45.0% | |
| !! | ATTCGTCTTTTTCACGCGCT+CGG | + | chr5_1:71041588-71041607 | None:intergenic | 45.0% |
| TTTCGTGCTTCTGTTCCATC+TGG | - | chr5_1:71041939-71041958 | Msa0747330:five_prime_UTR | 45.0% | |
| ! | TGACGGCGTCTTCGAGTTTA+CGG | + | chr5_1:71041672-71041691 | None:intergenic | 50.0% |
| TACGATGAAGAGAACGGGGT+CGG | + | chr5_1:71041719-71041738 | None:intergenic | 50.0% | |
| GTTCCATCTGGAAACTCCAC+TGG | - | chr5_1:71041951-71041970 | Msa0747330:five_prime_UTR | 50.0% | |
| ATGCCAGTGGAGTTTCCAGA+TGG | + | chr5_1:71041957-71041976 | None:intergenic | 50.0% | |
| ! | GACGGCGTCTTCGAGTTTAC+GGG | + | chr5_1:71041671-71041690 | None:intergenic | 55.0% |
| !!! | GTCGGAGGTTTTGGCTCTGA+CGG | + | chr5_1:71041701-71041720 | None:intergenic | 55.0% |
| !!! | GGAGGTTTTGGCTCTGACGG+CGG | + | chr5_1:71041698-71041717 | None:intergenic | 60.0% |
| ! | GAGAACGGGGTCGGAGGTTT+TGG | + | chr5_1:71041710-71041729 | None:intergenic | 60.0% |
| GATGAAGAGAACGGGGTCGG+AGG | + | chr5_1:71041716-71041735 | None:intergenic | 60.0% | |
| CACGCGCTCGGAGATGAGAG+TGG | + | chr5_1:71041576-71041595 | None:intergenic | 65.0% | |
| ! | GGCTCTGACGGCGGCATTGA+CGG | + | chr5_1:71041689-71041708 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 71041436 | 71041980 | 71041436 | ID=Msa0747330;Name=Msa0747330 |
| chr5_1 | mRNA | 71041436 | 71041980 | 71041436 | ID=Msa0747330-mRNA-1;Parent=Msa0747330;Name=Msa0747330-mRNA-1;_AED=0.01;_eAED=0.01;_QI=195|1|0.5|1|1|1|2|0|87 |
| chr5_1 | exon | 71041436 | 71041594 | 71041436 | ID=Msa0747330-mRNA-1:exon:21018;Parent=Msa0747330-mRNA-1 |
| chr5_1 | exon | 71041681 | 71041980 | 71041681 | ID=Msa0747330-mRNA-1:exon:21017;Parent=Msa0747330-mRNA-1 |
| chr5_1 | five_prime_UTR | 71041786 | 71041980 | 71041786 | ID=Msa0747330-mRNA-1:five_prime_utr;Parent=Msa0747330-mRNA-1 |
| chr5_1 | CDS | 71041681 | 71041785 | 71041681 | ID=Msa0747330-mRNA-1:cds;Parent=Msa0747330-mRNA-1 |
| chr5_1 | CDS | 71041436 | 71041594 | 71041436 | ID=Msa0747330-mRNA-1:cds;Parent=Msa0747330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
