AlfalfaGEDB | Msa0753180

M. sativa cultivar zhongmu-4 / Msa0753180

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0043621
PFAM: PF03479.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0753180	sp\|Q8GXB3\|AHL5_ARATH	48.958	384	127	13	1	321	1	378	2.51e-93	285

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0753180	G7KC38	95.639	321	14	0	1	321	1	321	0.0	630

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0292330	Msa0753180	0.847180	1.361609e-59	-8.615850e-47
Msa0483910	Msa0753180	0.803369	3.602330e-49	-8.615850e-47
Msa0689230	Msa0753180	0.908314	1.956514e-81	-8.615850e-47
Msa0753180	Msa1003800	0.801153	1.030080e-48	-8.615850e-47
Msa0753180	Msa1358220	0.913456	6.025238e-84	-8.615850e-47
Msa0753180	Msa1413690	0.840299	9.453408e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0753180	MtrunA17_Chr5g0397551	95.639	321	14	0	1	321	1	321	0.0	630
Msa0753180	MtrunA17_Chr8g0386741	64.306	353	92	8	1	321	1	351	3.75e-130	375
Msa0753180	MtrunA17_Chr8g0349091	48.649	259	124	5	69	321	88	343	5.57e-68	216
Msa0753180	MtrunA17_Chr7g0247431	43.503	354	159	13	1	321	1	346	1.46e-62	202
Msa0753180	MtrunA17_Chr4g0054661	50.521	192	76	6	70	249	73	257	1.39e-48	166
Msa0753180	MtrunA17_Chr7g0245841	45.575	226	114	4	68	289	58	278	1.25e-46	160
Msa0753180	MtrunA17_Chr1g0179461	44.286	210	103	3	66	261	65	274	3.75e-46	160
Msa0753180	MtrunA17_Chr8g0351761	41.923	260	132	5	68	312	69	324	3.38e-44	154
Msa0753180	MtrunA17_Chr4g0056761	49.519	208	92	6	70	264	72	279	1.60e-40	144
Msa0753180	MtrunA17_Chr1g0188211	45.370	216	94	7	59	251	37	251	3.16e-39	141

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0753180	AT1G63470.1	48.958	384	127	13	1	321	1	378	2.55e-94	285
Msa0753180	AT1G63480.2	47.059	340	125	11	1	294	1	331	5.59e-81	250
Msa0753180	AT1G63480.1	47.059	340	125	11	1	294	1	331	5.59e-81	250
Msa0753180	AT1G63480.5	46.407	334	124	11	7	294	1	325	1.74e-77	241
Msa0753180	AT1G63480.7	46.407	334	124	11	7	294	1	325	1.74e-77	241
Msa0753180	AT1G63480.4	46.407	334	124	11	7	294	1	325	1.74e-77	241
Msa0753180	AT1G63480.3	46.407	334	124	11	7	294	1	325	1.74e-77	241
Msa0753180	AT1G63480.6	46.407	334	124	11	7	294	1	325	1.74e-77	241
Msa0753180	AT2G45850.1	43.575	358	155	11	1	321	1	348	2.58e-73	230
Msa0753180	AT2G45850.2	43.575	358	155	11	1	321	1	348	2.58e-73	230

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Find 111 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCAAAATGGGATAAG+AGG	0.394158	5_2:-6893074	None:intergenic
AAAAGAAAAGAGGAAGACCT+AGG	0.598976	5_2:+6893552	Msa0753180:CDS
AAAAGAAAAGAGTTTGGTTG+TGG	0.471775	5_2:-6892945	None:intergenic
AAAAGTACACAACCACACAC+TGG	0.665666	5_2:+6896441	Msa0753180:CDS
AAACTCAAAATGGGATAAGA+GGG	0.580219	5_2:-6893073	None:intergenic
AAAGCTCTAAACCCAGGAGG+TGG	0.606520	5_2:-6893422	None:intergenic
AAATGCAGAGAAAACAGAAA+AGG	0.437943	5_2:-6893169	None:intergenic
AACCAGATAAGCATAATATT+TGG	0.258954	5_2:-6895625	None:intergenic
AACCCTAGATTTCAAAATCT+AGG	0.341061	5_2:+6893008	Msa0753180:five_prime_UTR
AACTATACAGCTTCTGGAAC+AGG	0.388590	5_2:+6896384	Msa0753180:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAAAAGAGTTTGGTTG+TGG	-	chr5_2:6892948-6892967	None:intergenic	30.0%
AACCCTAGATTTCAAAATCT+AGG	+	chr5_2:6893008-6893027	Msa0753180:five_prime_UTR	30.0%
ACCCTAGATTTCAAAATCTA+GGG	+	chr5_2:6893009-6893028	Msa0753180:five_prime_UTR	30.0%
AAACTCAAAATGGGATAAGA+GGG	-	chr5_2:6893076-6893095	None:intergenic	30.0%
AAAACTCAAAATGGGATAAG+AGG	-	chr5_2:6893077-6893096	None:intergenic	30.0%
GAAGAGAGAAAACTCAAAAT+GGG	-	chr5_2:6893085-6893104	None:intergenic	30.0%
TGAAGAGAGAAAACTCAAAA+TGG	-	chr5_2:6893086-6893105	None:intergenic	30.0%
AATGCAGAGAAAACAGAAAA+GGG	-	chr5_2:6893171-6893190	None:intergenic	30.0%
AAATGCAGAGAAAACAGAAA+AGG	-	chr5_2:6893172-6893191	None:intergenic	30.0%
TATAAAACACAACAATGGCA+AGG	-	chr5_2:6894121-6894140	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	6892919	6896850	6892919	ID=Msa0753180;Name=Msa0753180
chr5_2	mRNA	6892919	6896850	6892919	ID=Msa0753180-mRNA-1;Parent=Msa0753180;Name=Msa0753180-mRNA-1;_AED=0.02;_eAED=0.02;_QI=425\|1\|1\|1\|1\|1\|5\|365\|321
chr5_2	exon	6892919	6893710	6892919	ID=Msa0753180-mRNA-1:exon:3033;Parent=Msa0753180-mRNA-1
chr5_2	exon	6894400	6894467	6894400	ID=Msa0753180-mRNA-1:exon:3034;Parent=Msa0753180-mRNA-1
chr5_2	exon	6895156	6895287	6895156	ID=Msa0753180-mRNA-1:exon:3035;Parent=Msa0753180-mRNA-1
chr5_2	exon	6895617	6895775	6895617	ID=Msa0753180-mRNA-1:exon:3036;Parent=Msa0753180-mRNA-1
chr5_2	exon	6896246	6896850	6896246	ID=Msa0753180-mRNA-1:exon:3037;Parent=Msa0753180-mRNA-1
chr5_2	five_prime_UTR	6892919	6893343	6892919	ID=Msa0753180-mRNA-1:five_prime_utr;Parent=Msa0753180-mRNA-1
chr5_2	CDS	6893344	6893710	6893344	ID=Msa0753180-mRNA-1:cds;Parent=Msa0753180-mRNA-1
chr5_2	CDS	6894400	6894467	6894400	ID=Msa0753180-mRNA-1:cds;Parent=Msa0753180-mRNA-1
chr5_2	CDS	6895156	6895287	6895156	ID=Msa0753180-mRNA-1:cds;Parent=Msa0753180-mRNA-1
chr5_2	CDS	6895617	6895775	6895617	ID=Msa0753180-mRNA-1:cds;Parent=Msa0753180-mRNA-1
chr5_2	CDS	6896246	6896485	6896246	ID=Msa0753180-mRNA-1:cds;Parent=Msa0753180-mRNA-1
chr5_2	three_prime_UTR	6896486	6896850	6896486	ID=Msa0753180-mRNA-1:three_prime_utr;Parent=Msa0753180-mRNA-1

Gene Sequence

>Msa0753180

ATGGATGAGAGAGAAGCTATGTCATTTTCTGATGGGTCTGGTTCATATTACATGCATAGAGAAAGAGTGTTTCAACAACCACCTCCTGGGTTTAGAGCTTTGTCTAATCCTCATGGTGGTTCTGATGGTTCTGCATTTTCAGCAGAGCATCAACATGGTAGTTTTAGTCATGGTGCCGTAGTTCCTTATTCAGGGGAGCTGTCTGTGAAAAAGAAAAGAGGAAGACCTAGGAAGTATGGTCCTGATGTACCTGTTTCTTTGAGACTTTCTCCTATGTCTGCTACTGCTAATTCCACTCCTGATTCGGAGAAACGGCCGAGAGGACGGCCACCGGGAAGTGGAAGGAAGCAACAGCTAGCTGCTCTAGGTGAATGGATGAACAGTTCAGCTGGACAGGCTTTTTCACCTCATGTTATCACCATTGAAGCTCAAGAGGACATTGTAGCAAAGTTATTGTCATTTTCACAACATAGACCACGCGCTTTATGCATCTTGTCAGGAACTGGGACTGTATCTTCAGTCACTCTGCGCCAGCCTGCTTCTACCAGTGTCAGTGTTACATATGAGGGTCGATTCCAAATATTATGCTTATCTGGTTCTTTCTTGGTTGCTGAAGATGGTGGACCTCACAATAGAACTGGTGGCATTAGTGTTTCCCTTTCTAGTACGGATGGTCATGTTATCGGTGGTGGTGTTGCTAGGCTTATTGCTGCAAGCCCTGTCCAGGTGGTAGTTTGCAGCTTTGTGTACGGTGGTTCTAAGCCAAAGACAACAAAACAAGAAACTGCTGCAAAAGAAGATGATGATTCTGAGCCTCAGAGTAGTGACAAATTAGCTTCTCCAGGAAGTGAGCCACCTAATCAAAACTATACAGCTTCTGGAACAGGCACAATGTGGCATGGATCAAGGACAGTAGACGTGAAAAGTACACAACCACACACTGGTATTGATTTGATGAACGGGTGA

Protein sequence

>Msa0753180

MDEREAMSFSDGSGSYYMHRERVFQQPPPGFRALSNPHGGSDGSAFSAEHQHGSFSHGAVVPYSGELSVKKKRGRPRKYGPDVPVSLRLSPMSATANSTPDSEKRPRGRPPGSGRKQQLAALGEWMNSSAGQAFSPHVITIEAQEDIVAKLLSFSQHRPRALCILSGTGTVSSVTLRQPASTSVSVTYEGRFQILCLSGSFLVAEDGGPHNRTGGISVSLSSTDGHVIGGGVARLIAASPVQVVVCSFVYGGSKPKTTKQETAAKEDDDSEPQSSDKLASPGSEPPNQNYTASGTGTMWHGSRTVDVKSTQPHTGIDLMNG*